CN217717432U

CN217717432U - Optical detection assembly and optical detection system

Info

Publication number: CN217717432U
Application number: CN202221434471.XU
Authority: CN
Inventors: 杜京师
Original assignee: Zhongyuan Huiji Biotechnology Co Ltd
Current assignee: Zhongyuan Huiji Biotechnology Co Ltd
Priority date: 2022-06-08
Filing date: 2022-06-08
Publication date: 2022-11-01
Anticipated expiration: 2032-06-08

Abstract

The utility model provides an optics detecting element and optics detecting system belongs to the optics check out test set field. An optical detection assembly comprising: a light detector for detecting a light signal; a light collection unit for collecting the light signal and projecting the collected light signal on a light detector; a direct stop diaphragm disposed in front of the light collection unit; the aperture diaphragm is arranged between the light collection unit and the light detector; wherein the light collecting unit is an aspheric lens. An optical detection system comprising: a flow cell for passage of the detected blood cells; the front light assembly is used for generating laser beams and focusing the laser beams on the flow chamber to form forward scattered light, side scattered light and side fluorescent light; the lateral detection assembly is used for detecting lateral scattered light and lateral fluorescence; and a forward detection assembly, which is the optical detection assembly and is used for detecting forward scattered light. The utility model discloses can avoid producing spherical aberration, astigmatism problem, improve the detection precision to the blood cell.

Description

Optical detection assembly and optical detection system

Technical Field

The utility model belongs to the optical detection equipment field especially relates to an optical detection subassembly and optical detection system.

Background

In the existing blood cell analyzer, the cells are generally classified and counted by using a laser scattering fluorescence staining method. A laser is used as a light source, and the fluorescent marked cells in the flow chamber are irradiated after the collimated focusing of a lens to generate forward scattered light, side scattered light and side fluorescent light.

The forward scattering light reflects the size of the cell, the forward scattering light reflects the complexity in the cell, and the lateral fluorescence reflects the content of nucleic acid in the cell, wherein the nucleic acid comprises deoxyribonucleic acid and ribonucleic acid. The scattered light is converted into an electric signal through the optical detection assembly, and the electric signal is analyzed to realize the classification of blood cells. Currently, most of optical detection assemblies employ spherical lenses or spherical lens groups to collect and converge forward scattered light signals, and then detect the forward scattered light signals. The spherical lens is subject to its own structure, and tends to cause problems such as spherical aberration and astigmatism when converging forward scattered light.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned shortcomings of the prior art, an object of the present invention is to provide an optical inspection module and an optical inspection system, which are used to solve the problems of spherical aberration, astigmatism, etc. easily occurring in the optical inspection module in the prior art.

To achieve the above and other related objects, the present invention provides an optical inspection assembly, comprising:

a light detector for detecting a light signal;

a light collection unit for collecting light signals and projecting the collected light signals on the light detector;

the straight stopping diaphragm is arranged in front of the light collecting unit and used for screening the light signals entering the light collecting unit;

the aperture diaphragm is arranged between the light collection unit and the light detector and is used for filtering the light signal projected on the light detector by the light collection unit;

wherein the light collection unit is an aspheric lens.

Optionally, the light collection unit has an incident surface and an exit surface, the incident surface is a plane or a convex surface, and the exit surface is a convex surface.

Optionally, the surface reflectivity of the incident surface is less than or equal to 0.1%.

Optionally, the surface reflectivity of the incident surface is less than or equal to 0.05%.

Optionally, the focal length of the light collection unit is 5mm to 13mm.

Optionally, the focal length of the light collection unit is 7mm to 11mm.

Optionally, the clear aperture of the light collection unit is > 3mm and the numerical aperture of the light collection unit is > 0.2.

Optionally, the clear aperture of the light collection unit is > 5mm and the numerical aperture of the light collection unit is > 0.25.

Optionally, the light collection unit is an even aspheric lens.

Optionally, the light collection unit is spaced from the straight stop by a distance of < 1mm.

Optionally, the straight-blocking diaphragm screens an optical signal with an incident angle between an upper limit and a lower limit, where the lower limit is 1 ° and the upper limit is greater than or equal to 5 °.

Optionally, the upper limit is ≧ 9 °.

Optionally, the aperture diaphragm has a first through hole and a second through hole, both the first through hole and the second through hole are in a long strip shape, the first through hole intersects with the second through hole, the length of the first through hole is greater than or equal to 3mm, and the width of the first through hole is less than or equal to 1.5mm.

Optionally, the length of the first through hole is greater than or equal to 5mm, and the width of the first through hole is less than or equal to 1mm.

Optionally, the aspheric lens is 1

The utility model also provides an optical detection system, include:

a flow chamber for passage of the detected blood cells;

the front light assembly is used for generating a laser beam and focusing the laser beam on the flow chamber along a first direction, forming forward scattering light along the first direction, and forming side scattering light and side fluorescence along a second direction, wherein the first direction and the second direction are intersected;

a lateral detection assembly for detecting the lateral scattered light and the lateral fluorescence; and

the forward detection assembly is the optical detection assembly and is used for detecting the forward scattered light.

As described above, the utility model provides a pair of optical detection subassembly and optical detection system has following beneficial effect: because the light collection unit is arranged and is an aspheric lens, compared with a spherical lens, the aspheric lens can avoid the problems of spherical aberration and astigmatism generated by the convergent scattering light of the spherical lens, and the detection precision of blood cells is improved. And the straight blocking diaphragm is arranged before the light collecting unit and can screen and enter the light signal of the light collecting unit, so that direct light is reduced, and the direct light is prevented from entering the light detector to cause signal saturation. Meanwhile, the aperture diaphragm is arranged between the light collection unit and the optical detector, and can filter the optical signal projected on the optical detector by the light collection unit, so that the interference of stray light on the optical detector is avoided, and the detection quality of the optical detector is improved.

Drawings

Fig. 1 is a schematic structural diagram of an optical inspection system according to an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a stop straight diaphragm in an embodiment of the present invention.

Fig. 3 is one of schematic structural diagrams of a light collection unit according to an embodiment of the present invention.

Fig. 4 is a second schematic structural diagram of a light collecting unit according to an embodiment of the present invention.

Fig. 5 is a schematic structural diagram of an aperture stop according to an embodiment of the present invention.

Description of reference numerals:

the device comprises a laser 1, a collimating mirror 2, a shaping lens group 3, a straight blocking diaphragm 4, a light collecting unit 5, an aperture diaphragm 6, a light detector 7, a lateral collecting lens 8, a dichroic mirror 9, a lateral scattering detection component 10, a lateral fluorescence detection component 11, a front light component 101, an optical isolation component 102, a flow chamber 103, a front detection component 104, a lateral detection component 105, a light blocking strip 401, a diaphragm hole 402, an incident surface 501, an emergent surface 502, a first through hole 601 and a second through hole 602.

Detailed Description

The following description is provided for illustrative purposes, and other advantages and features of the present invention will become apparent to those skilled in the art from the following detailed description.

Please refer to fig. 1 to 5. It should be understood that the structure, proportion, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions of the present invention, so that the present invention does not have the substantial technical significance, and the modification of any structure, the change of the proportion relation or the adjustment of the size should still fall within the scope of the technical content disclosed in the present invention without affecting the function and the achievable purpose of the present invention. Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.

As shown in fig. 1 to 5, the present embodiment provides an optical detection assembly including a light detector 7, a light collection unit 5, a straight-blocking diaphragm 4, and a straight-blocking diaphragm 4. The light detector 7 is used for detecting the light signal, and the light collecting unit 5 is an aspheric lens for collecting the light signal and projecting the collected light signal on the light detector 7. A straight stop aperture 4 is arranged in front of the light collection unit 5 for screening the light signal entering the light collection unit 5. An aperture 6 is disposed between the light collection unit 5 and the light detector 7 for filtering the light signal projected by the light collection unit 5 onto the light detector 7.

The light collecting unit 5 is an aspheric lens, which can avoid the problems of spherical aberration and astigmatism caused by the convergence of scattered light compared with a spherical lens, thereby improving the detection accuracy of blood cells.

In some embodiments, the light collection unit 5 employs two or more aspheric lenses, or a combination of a spherical lens and an aspheric lens. In this embodiment, the light collection unit 5 is used to collect the scattered light signals in the forward direction within a low angle and focus the scattered light signals at the forward detector position, and the light collection unit 5 is an aspheric lens, and the number of the light collection units is 1. Compared with the embodiment of the spherical lens, the embodiment adopts the aspherical mirror, can effectively improve the problems of spherical aberration and astigmatism caused by the spherical mirror, is more beneficial to forward light path debugging, and enables the optical detection system to reach an ideal state more easily. Meanwhile, a single aspherical mirror is adopted, so that a light path system is simpler, and the miniaturization of an optical detection system is facilitated on the basis of saving cost. And after a single aspheric lens is adopted, the mechanical structure of the optical detection component is simpler, and the requirements on the mechanical structure precision and the light path debugging are lower.

In this embodiment, the direct-blocking diaphragm 4 is disposed in front of the light collection unit 5, and can screen the light signal entering the light collection unit 5, reduce direct light, and avoid signal saturation caused by the direct light signal entering the light detector 7. Meanwhile, the aperture diaphragm 6 is arranged between the light collection unit 5 and the optical detector 7, so that stray light in an optical signal projected to the optical detector 7 by the light collection unit 5 can be filtered, the interference of the stray light on the optical detector 7 is avoided, and the detection quality of the optical detector 7 is improved.

As shown in fig. 3, the light collection unit 5 has an incident surface 501 and an exit surface 502. The surface reflectivity of the incident surface 501 is less than or equal to 0.1% to reduce the reflection of the incident light signal, thereby reducing the influence of the reflected light on the stability of the light source. In this embodiment, the surface reflectivity of the incident surface 501 is less than or equal to 0.05%, such as 0.04%, to further reduce the influence of the reflected light on the stability of the light source.

In some embodiments, the entrance surface 501 is a plane and the exit surface 502 is a convex surface. The straight stop diaphragm 4 cooperates with the incident surface 501 to let the filtered light signal enter the light collection unit 5 through the incident surface 501. Compared with a convex surface, the thickness of the light collection unit 5 with the plane incident surface 501 is smaller, which is beneficial to reducing the size of the optical detection assembly. Meanwhile, the light collection unit 5 is usually fixed by using glue, and the glue is usually coated on the light collection unit 5 and then fixed by bonding. When glue is squeezed on the entrance face 501, the glue easily flows away if the entrance face 501 is convex, and the glue can better stay on the entrance face 501 if the entrance face 501 is flat, facilitating the mounting of the light collecting unit 5.

As shown in fig. 4, in another embodiment, the incident surface 501 and the exit surface 502 are both convex surfaces, and when light is incident, most of the reflected light generated by the convex surfaces deviates from the incident direction, and less reflected light is returned to the front light assembly 101 along the incident direction, so as to reduce the influence of the reflected light on the stability of the light source.

In order to shorten the optical path size of the optical detection component, the focal length of the aspheric lens is between 5mm and 13mm. In this embodiment, the focal length of the aspherical lens ranges from 7mm to 11mm. Meanwhile, the distance between the aspheric lens incidence surface 501 and the straight stop diaphragm 4 is less than 1mm. To further reduce the optical path size of the optical detection assembly.

In particular, in the present embodiment, the focal length of the light collection unit 5 is between 7mm and 11mm, for example 8mm.

In some embodiments, the numerical aperture of the light collection unit 5 needs to be > 0.2 and the clear aperture of the light collection unit 5 > 3mm to ensure that the scattered light transmitted through the stop 4 is effectively received and focused onto the position of the light detector 7. In this embodiment, the numerical aperture of the light collection unit 5 is > 0.25, e.g. 0.3, and the clear aperture of the light collection unit 5 is > 5mm, e.g. 6mm. To further increase the scattered light reception range of the light collection unit 5.

In some embodiments, the light collection unit 5 employs an even-order aspheric lens, which is simpler to process and effectively reduces the processing cost of the aspheric lens compared with an odd-order aspheric lens.

The straight-blocking diaphragm 4 can filter and enter the light signal with the incidence angle between the upper limit and the lower limit into the light collection unit 5. In some embodiments, the optical signal with the lower limit of 1 ° and the upper limit of 5 ° or more is direct light, which, if entering the light collection unit 5, would cause the optical signal of the optical detector 7 to be saturated, affecting the detection result. The upper limit should be as high as possible, at least not less than 5 °, in this embodiment, the upper limit is greater than or equal to 9 °, for example, 10 °, to increase the reception of optical signals other than direct light as much as possible.

Specifically, as shown in fig. 2, the straight stop 4 includes a circular stop hole 402 and a light-blocking strip 401 disposed in the middle of the stop hole 402, and the stop hole 402 is circular in design, so that it can selectively receive forward scattered light signals with low angles, for example, scattered light within 10 °. The light-blocking strip 401 can block direct light in a low angle direction, e.g., within 1 °, after the laser beam irradiates the flow cell 103, preventing forward signal saturation. Meanwhile, the light blocking strips 401 can absorb or diffuse the direct light through the matte blackening treatment, so as to prevent the direct light from returning to the front light assembly 101 and affecting the stability of the optical detection system.

As shown in fig. 5, the aperture 6 has a first through hole 601 and a second through hole 602, the first through hole 601 and the second through hole 602 are both elongated, the first through hole 601 intersects with the second through hole 602, the optical signal collected by the first light collecting assembly can be projected onto the optical detector 7 through the first through hole 601 and the second through hole 602, and the stray light outside the first through hole 601 and the second through hole 602 is filtered. In this embodiment, the first through hole 601 and the second through hole 602 are both waist holes, and the first through hole 601 and the second through hole 602 intersect at the midpoint and are perpendicular to each other. In some embodiments, the first through hole 601 is long-strip-shaped, the length of the first through hole 601 is greater than or equal to 3mm, and the width of the first through hole 601 is less than or equal to 1.5mm, so that all required low-angle scattered light can pass through the small-hole diaphragm 6, the purpose of blocking stray light is achieved, and effective identification of forward tiny light signals is guaranteed. In this embodiment, the length of the first through hole 601 is greater than or equal to 5mm, for example, 6mm. The width of the first through hole 601 is less than or equal to 1mm, for example 0.7mm. So as to further improve the screening and filtering capability of the first through hole 601 for the optical signal gathered by the first light collection component.

As shown in fig. 1, the present embodiment further provides an optical detection system, which includes a flow cell 103, a front light assembly 101, a lateral detection assembly, and a forward detection assembly.

The flow chamber 103 is used for blood cells to be detected to pass through, and the blood cells pass through the flow chamber 103 one by one, so that analysis and detection are facilitated. The front light assembly 101 is used for generating a laser beam and focusing the laser beam on the flow chamber 103 along a first direction, and after the laser beam is focused in the flow chamber, forward scattered light is formed along the first direction, and side scattered light and side fluorescent light are formed along a second direction. In some embodiments, the first direction intersects the second direction. In this embodiment, the first direction is perpendicular to the second direction. The lateral detection assembly is used for detecting lateral scattered light and lateral fluorescence. The forward detection assembly is the optical detection assembly for detecting forward scattered light.

In some embodiments, the front light assembly 101 includes a laser 1, a collimating lens 2, and a shaping lens group 3, wherein laser light generated by the laser 1 passes through the collimating lens 2 to form parallel light, passes through the shaping lens group 3, and irradiates a laser beam onto the flow chamber 103 to irradiate blood cells to be detected, and then generates forward scattered light, side scattered light, and side fluorescent light.

The laser 1 of the front light assembly 101 has a laser output wavelength of 620nm to 650nm and an elliptical laser output spot. Laser output by the laser 1 forms parallel light with elliptic light spots after passing through the collimating lens. The parallel light enters the shaping lens group 3, and the light spot is enabled to reach the proper size form at the position of the flow chamber 103 through the action of the shaping lens.

Specifically, the incident direction of the parallel light is the Z direction, the emission directions of the side scattered light and the side fluorescent light are the Y direction perpendicular to the Z direction, and the directions perpendicular to the Y direction and the Z direction are the X direction. In this embodiment, the first through hole 601 of the aperture 6 is arranged along the X direction, and the second through hole 602 of the aperture 6 is arranged along the Y direction. The spot size of the collimated light can ensure that the sample in the flow cell 103 is covered in the X-direction and scattered light is generated when a single cell is illuminated in the Y-direction as the cell in the flow cell 103 passes through the detection zone. In this embodiment, the spot of the collimated light at the location of the flow cell 103 has a size in the Y direction of between 5 μm and 20 μm, for example, 12 μm. The dimension in the X direction is > 150. Mu.m, for example 175. Mu.m.

In some embodiments, an optical isolator assembly 102, i.e., an optical isolator, is also mounted between the front light assembly 101 and the flow cell 103. Optical isolator assembly 102 can prevent reflected light from flow cell 103 and other device surfaces from returning into laser 1 by changing the polarization of incident and reflected light, which can affect the stability of laser 1 and cause a reduction in the sensitivity of the optical detection system.

The lateral detection assembly comprises a lateral collection lens 8, a dichroic mirror 9, a lateral scattering detection assembly 10 and a lateral fluorescence detection assembly 11, and the lateral detection assembly can detect and collect lateral scattered light and lateral fluorescence. The side scattering detection assembly and the side fluorescence detection assembly 11 share the side collecting lens 8, so that the high-angle scattered light and the fluorescence pass through the collecting lens and are focused on the detection assembly to complete detection. The dichroic mirror 9 is configured to distinguish side scattered light from side fluorescent light, and specifically, the light signal entering the dichroic mirror 9 includes the side scattered light and the side fluorescent light, where the side scattered light is consistent with the wavelength of the light source laser, and the side fluorescent light is longer than the wavelength of the laser light generated by the light source laser 1. Thus, the dichroic mirror 9 is able to reflect the side-scattered light into the side scatter detection assembly 10. The side scatter detection assembly 10 can obtain information reflecting the complexity of the cell according to the size of the side scatter signal and the pulse width parameter. Meanwhile, the dichroic mirror 9 allows lateral fluorescence to transmit through and enter the lateral fluorescence detection assembly, and the lateral fluorescence detection assembly can acquire information reflecting the content of nucleic acid in cells according to the size, the intensity and the pulse width of a fluorescence signal.

In summary, in the optical detection assembly and the optical detection system provided by this embodiment, the direct-blocking diaphragm 4 is disposed in front of the light collection unit 5, and can screen the light signal entering the light collection unit 5, so as to reduce direct light and avoid the direct light signal entering the light detector to cause saturation of the light signal. Meanwhile, the aperture diaphragm 6 is arranged between the light collection unit 5 and the light detector 7, so that stray light in an optical signal projected on the light detector 7 by the light collection unit 5 can be filtered, the interference of the stray light on the light detector 7 is avoided, and the detection quality of the light detector 7 is improved. The light collecting unit 5 is an aspheric lens which can avoid the problems of spherical aberration and astigmatism caused by the convergence of scattered light by the spherical lens and improve the detection precision of blood cells. Meanwhile, the optical path system adopting the aspherical mirror is simpler, and the miniaturization of the optical detection system is facilitated on the basis of saving the cost. And after a single aspheric lens is adopted, the mechanical structure of the optical detection component is simpler, the requirements on the precision of the mechanical structure and the debugging of the optical path are lower, the forward optical path debugging is facilitated, and the optical detection system can reach an ideal state more easily.

The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims

1. An optical inspection assembly, comprising:

a light detector for detecting a light signal;

wherein the light collection unit is an aspheric lens.

2. The optical inspection assembly of claim 1, wherein: the light collection unit is provided with an incident surface and an emergent surface, wherein the incident surface is a plane or a convex surface, and the emergent surface is a convex surface.

3. The optical inspection assembly of claim 2, wherein: the surface reflectivity of the incident surface is less than or equal to 0.1 percent.

4. The optical inspection assembly of claim 2, wherein: the surface reflectivity of the incident surface is less than or equal to 0.05 percent.

5. The optical inspection assembly of claim 1, wherein: the focal length of the light collection unit is 5 mm-13 mm.

6. The optical inspection assembly of claim 5, wherein: the focal length of the light collection unit is 7 mm-11 mm.

7. The optical inspection assembly of claim 1, wherein: the clear aperture of the light collection unit is larger than 3mm, and the numerical aperture of the light collection unit is larger than 0.2.

8. The optical inspection assembly of claim 7, wherein: the clear aperture of the light collection unit is larger than 5mm, and the numerical aperture of the light collection unit is larger than 0.25.

9. The optical inspection assembly of claim 1, wherein: the light collection unit is an even aspheric lens.

10. The optical inspection assembly of claim 1, wherein: the distance between the light collecting unit and the straight blocking diaphragm is less than 1mm.

11. The optical inspection assembly of claim 1, wherein: the light signal with the straight blocking diaphragm screening incidence angle between the upper limit and the lower limit enters the light collecting unit, the lower limit is 1 degree, and the upper limit is larger than or equal to 5 degrees.

12. The optical detection assembly of claim 11, wherein: the upper limit is more than or equal to 9 degrees.

13. The optical inspection assembly of claim 1, wherein: the aperture diaphragm is provided with a first through hole and a second through hole, the first through hole and the second through hole are both in long strips, the first through hole is intersected with the second through hole, the length of the first through hole is larger than or equal to 3mm, and the width of the first through hole is smaller than or equal to 1.5mm.

14. The optical inspection assembly of claim 13, wherein: the length of the first through hole is more than or equal to 5mm, and the width of the first through hole is less than or equal to 1mm.

15. The optical inspection assembly of claim 1, wherein: the number of the aspheric lenses is 1.

16. An optical inspection system, comprising:

a flow cell for passage of the detected blood cells;

a forward detection assembly, being an optical detection assembly as claimed in any one of claims 1 to 15, for detecting said forward scattered light.