CN217687590U - Temperature-sensitive probe and temperature testing device of digital PCR instrument - Google Patents
Temperature-sensitive probe and temperature testing device of digital PCR instrument Download PDFInfo
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- CN217687590U CN217687590U CN202221788582.0U CN202221788582U CN217687590U CN 217687590 U CN217687590 U CN 217687590U CN 202221788582 U CN202221788582 U CN 202221788582U CN 217687590 U CN217687590 U CN 217687590U
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Abstract
The utility model discloses a temperature sensing probe and digital PCR appearance temperature-measuring device belongs to temperature test technical field, include: a probe base; the elastic device is connected with the probe base; a heat transfer part connected to the probe base; a temperature sensor located inside the temperature transmitting part and in contact with the temperature transmitting part; the probe base is provided with an elastic object fixing groove for fixing the elastic device; according to the temperature-sensitive probe and the temperature testing device of the digital PCR instrument, the metal heat conduction layer is tightly attached to the digital PCR instrument through the temperature-sensitive probe and the elastic device, the function of supporting the module is realized through 4 probes, the function of starting to awaken the module is automatically identified, and meanwhile, the function of quickly returning data is realized; meanwhile, the probe is combined in a use mode, a plurality of optional accessories are combined for use, and the temperature efficiency and the measurement precision are improved.
Description
Technical Field
The utility model belongs to the technical field of the temperature test, concretely relates to temperature sensitive probe and digital PCR appearance temperature-measuring device.
Background
Digital PCR (dPCR), is a quantitative analysis technique that has been rapidly developed in recent years, and the result determination does not depend on the cycle threshold (Ct) of the amplification curve, is not affected by the amplification efficiency, has excellent accuracy and reproducibility, and can realize absolute quantitative analysis; the digital PCR instrument has shown huge technical advantages and application prospects in the research fields of nucleic acid detection, identification and the like, uniformly disperses nucleic acid to be detected into a large number of mutually separated micro reaction units through limit dilution, each reaction unit contains one or zero nucleic acid molecules to be detected, after PCR reaction, a terminal fluorescence signal is collected, and absolute quantitative analysis is carried out on the initial concentration of the nucleic acid based on Poisson distribution; in the PCR reaction process, DNA is changed into a single strand when the temperature is high at 95 ℃ in vitro, the temperature is low, the primer and the single strand are combined according to the base complementary pairing principle when the temperature is normally about 60 ℃, then the temperature is adjusted to about 72 ℃ which is the optimal reaction temperature of the DNA polymerase, the DNA polymerase synthesizes a complementary strand along the direction from phosphoric acid to pentose, and the digital PCR instrument repeats the circulation until the sufficient copy number for analysis is obtained, and detection and quantitative analysis are carried out; the performance of temperature control is an important technical index of the digital PCR instrument, and the importance of testing and calibrating the digital PCR instrument is increasingly prominent;
the general administration of national quality supervision, inspection and quarantine in 2015 publishes and implements a 'JJF 1527-2015 polymerase chain reaction analyzer calibration standard', wherein requirements for point position, temperature indication error, temperature uniformity, temperature rising and falling rate and the like of temperature measurement of a PCR instrument are specified, however, the digital PCR instrument has various forms such as a micro-drop type, a chip type and a micro-pore type in function realization, the heating pores of the traditional PCR instrument are mostly designed by 0.1ml or 0.2ml 96 pore plates, a temperature measurement device aiming at the traditional PCR instrument mostly takes a whole-plate type temperature calibration plate as a main part, the temperature calibration plate is provided with a plurality of metal temperature measurement probes, the probes are similar to 0.1ml or 0.2ml PCR reaction plate in appearance, and the front ends of the probes are generally conical so as to be tightly attached to the heating pores; however, the conventional thermometric device has the following problems when the digital PCR instrument is used for measurement:
1. the heating surface of the digital PCR instrument is often a metal plane and is not designed with a conical hole, and a temperature measuring probe of the traditional PCR instrument cannot be tightly attached to the heating surface and cannot accurately measure temperature;
2. the sizes of the heating metal planes of the digital PCR instruments of different models are the same, the required temperature measuring points are different, and the traditional temperature measuring device cannot flexibly configure a measuring probe according to the size of the heating metal plane of the digital PCR instrument;
3. the measurement cannot be carried out in a closed space;
therefore, aiming at the problem that the temperature testing and calibrating device of the traditional PCR instrument cannot meet the temperature testing requirements of the digital PCR instrument, particularly of a chip type and a micropore type, a new temperature-sensitive probe and a temperature testing device of the digital PCR instrument need to be developed to solve the existing problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a temperature sensing probe and digital PCR appearance temperature-measuring device to solve the unable inseparable laminating of temperature sensing probe and the unable measured problem of combination formula.
In order to achieve the above purpose, the utility model provides a following technical scheme: a temperature-sensitive probe, comprising:
a probe base;
the elastic device is connected with the probe base;
a heat transfer part connected to the probe base;
a temperature sensor located inside the temperature transmitting part and in contact with the temperature transmitting part;
wherein, the probe base is provided with an elastic object fixing groove for fixing the elastic device.
Preferably, the temperature transfer part is a metal heat conduction layer, and the surface of the temperature transfer part is of a planar structure.
Preferably, the cross section of the heat transfer part is of an inverted T shape, and the edge protruding after the heat transfer part is embedded into one end part of the probe base is abutted against the end face of the probe base.
The utility model provides a digital PCR appearance temperature-measuring device in addition, include:
a housing;
a circuit board mounted in mating relation with the housing;
a battery connected to the circuit board;
a limiting structural member matched with the circuit board;
preparing the temperature-sensitive probe installed in the limiting structural part;
wherein, the temperature-sensitive probe is connected with the circuit board.
Preferably, an elastic device is installed in the elastic fixing groove of the temperature-sensitive probe, and the elastic device abuts against the shell, so that the temperature-sensitive probe penetrates through the limiting structural member to move.
Preferably, when 12 temperature-sensitive probes are arranged in the apparatus, the arrangement is 3 × 4.
Preferably, when 9 temperature-sensitive probes are arranged in the apparatus, the arrangement is 3 × 3.
Preferably, the circuit board is provided with a temperature acquisition circuit, an MCU master control circuit, a storage device, a wireless transceiver circuit and a power management module, wherein the temperature acquisition circuit is connected with the temperature sensor and also connected with the MCU master control circuit, and the MCU master control circuit is connected with the storage device, the wireless transceiver circuit and the power management module.
Preferably, a temperature-sensitive probe shell is arranged in the limiting hole of the limiting structural member, and the temperature-sensitive probe shell is sleeved on the temperature-sensitive probe.
Preferably, the battery is mounted on the circuit board, and the housing is provided at a removal opening for repairing or removing the battery.
The utility model discloses a technological effect and advantage: according to the temperature-sensitive probe and the temperature testing device of the digital PCR instrument, the temperature-sensitive probe and the elastic device are used for enabling the metal heat conduction layer to be tightly attached to the digital PCR instrument, the function of supporting the module is achieved through 4 probes, the function of starting to awaken the module is automatically identified, and meanwhile the function of rapidly returning data is achieved; meanwhile, the probe is combined in a use mode, a plurality of optional accessories are combined for use, and the temperature efficiency and the measurement precision are improved.
Drawings
Fig. 1 is a schematic structural view of a temperature-sensitive probe of the present invention;
FIG. 2 is an exploded view of the present invention;
fig. 3 is a perspective view of the present invention.
In the figure: 100. a probe base; 110. an elastic object fixing groove; 120. a screw; 130. a metal heat conducting layer; 200. a temperature sensor; 300. an elastic device; 400. a housing; 410. a disassembly port; 500. a limiting structural member; 600. a circuit board; 700. a temperature sensitive probe housing; 800. a battery.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a temperature sensitive probe as shown in figure 1, include:
a probe base 100;
an elastic means 300 connected to the probe mount 100;
a heat transfer part connected to the probe base 100;
a temperature sensor 200 located inside the temperature transmitting portion and contacting therewith; when the temperature sensor is used, the temperature transmission part is tightly attached to a measured object, and the temperature sensor 200 acquires a temperature signal;
the probe base 100 is provided with an elastic fixing groove 110 for fixing the elastic device 300, and when the probe base is used, if a measured object is non-planar, the elastic device 300 enables the temperature transmitting part to be tightly attached to the measured object through elasticity;
in this embodiment, the temperature transmitting portion is a metal heat conducting layer 130, and the surface thereof is a planar structure.
The cross section of the heat transfer part is of an inverted T shape, and the edge protruding after the heat transfer part is embedded into one end part of the probe base 100 is abutted against the end surface of the probe base 100; the probe base 100 is a hollow mushroom-shaped structure;
the utility model discloses in addition provide like figure 2, 3 a digit PCR appearance temperature-measuring device, include:
a housing 400;
a circuit board 600 mounted to be fitted to the housing 400;
a temperature acquisition circuit, an MCU master control circuit, a memory device, a wireless transceiver circuit, a power management module and peripheral equipment, such as a startup and shutdown key and an indicator light, are arranged on the circuit board 600, and the wireless transceiver mode has the advantages of higher test speed and simpler installation mode; the temperature acquisition circuit is connected with the temperature sensor 200, and is also connected with the MCU main control circuit which is connected with the memory device, the wireless transceiving circuit and the power management module;
the temperature acquisition part adopts a high-precision temperature sensor 200, and the MCU master control circuit acquires temperature data of the temperature sensor 200 and processes the data;
the MCU master control circuit consists of an MCU chip and a peripheral circuit thereof, wherein the peripheral circuit of the MCU chip comprises a power supply chip and a crystal oscillator; the MCU chip supports a wireless communication protocol and simultaneously supports a Bluetooth or ZigBee protocol, an antenna matching circuit and a PCB on-board antenna are added to peripheral devices of the MCU chip, and control software of the all-in-one machine is burned in the MCU chip or a peripheral Flash storage chip and controls the working flow of the all-in-one machine;
the memory device is generally a Flash memory chip or an EEPROM chip and is used for storing data such as equipment information, calibration data, factory information, test data and the like under the control of the MCU;
the wireless transceiving circuit mainly comprises an MCU chip supporting wireless transceiving function, a peripheral antenna matching circuit thereof and a PCB on-board antenna, and transmits the acquired temperature data to the upper computer;
a power management module: the main power supply is converted into the voltage required by each module of the system through a power conversion chip, a DC/DC chip or an LDO chip, and the power supply of a certain module is started or closed according to the requirement, so that the flexibility of power management is improved, and particularly, the low-power management capability of the all-in-one machine is improved;
a battery 800 connected to the circuit board 600; the battery 800 is mounted on the circuit board 600, and the housing 400 is disposed at a removal opening 410 for repairing or removing the battery 800; the circuit board 600 is fixedly mounted on the housing 400 by screws 120;
a limit structure 500 mounted in a matching manner with the circuit board 600; a temperature-sensitive probe shell 700 is arranged in a limiting hole of the limiting structural member 500, and the temperature-sensitive probe shell 700 is sleeved on the temperature-sensitive probe.
Preparing a temperature-sensitive probe installed in the limiting structural member 500;
wherein, the temperature sensor 200 in the temperature sensitive probe is connected with the circuit board 600. An elastic device 300 is installed in the elastic fixing groove 110 of the temperature-sensitive probe, and the elastic device 300 abuts against the shell 400, so that the temperature-sensitive probe penetrates through the limiting structural member 500 to move, in this embodiment, when 12 temperature-sensitive probes are configured in the elastic device 300 which is a spring, the arrangement mode is 3 × 4;
when the device is provided with 9 temperature-sensitive probes, the arrangement is 3 multiplied by 3; in this embodiment, if a plurality of temperature-sensitive probes are configured, they may be arranged in 4 × 4 or other arrangement manners;
the temperature acquisition circuit is also connected with the MCU master control circuit, and the MCU master control circuit is connected with the storage device, the wireless transceiver circuit and the power management module.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (10)
1. A temperature-sensitive probe, characterized by: the method comprises the following steps:
a probe base;
the elastic device is connected with the probe base;
a heat transfer part connected to the probe base;
a temperature sensor located inside the temperature transmitting part and in contact with the temperature transmitting part;
wherein, the probe base is provided with an elastic object fixing groove for fixing the elastic device.
2. A temperature-sensitive probe according to claim 1, wherein: the temperature transmission part is a metal heat conduction layer, and the surface of the temperature transmission part is of a plane structure.
3. A temperature-sensitive probe according to claim 1, wherein: the cross section of the heat transfer part is of an inverted T shape, and the edge protruding from the back of the heat transfer part is embedded into one end part of the probe base and is abutted against the end face of the probe base.
4. The utility model provides a digit PCR appearance temperature test device which characterized in that: the method comprises the following steps:
a housing;
a circuit board mounted in mating relation with the housing;
a battery connected to the circuit board;
a limiting structural member matched with the circuit board;
preparing a temperature-sensitive probe according to any one of claims 1-3 mounted in a spacing structure;
wherein, the temperature-sensitive probe is connected with the circuit board.
5. The digital PCR instrument temperature testing device of claim 4, wherein: and an elastic device is arranged in an elastic object fixing groove of the temperature-sensitive probe and is abutted against the shell, so that the temperature-sensitive probe penetrates through the limiting structural member to move.
6. The digital PCR instrument temperature testing device of claim 4, wherein: when the device is provided with 12 temperature-sensitive probes, the arrangement is 3 multiplied by 4.
7. The digital PCR instrument temperature testing device of claim 4, wherein: when the device is provided with 9 temperature-sensitive probes, the arrangement is 3 multiplied by 3.
8. The digital PCR instrument temperature testing device of claim 4, wherein: the circuit board is provided with a temperature acquisition circuit, an MCU master control circuit, a storage device, a wireless transceiving circuit and a power management module, wherein the temperature acquisition circuit is connected with the temperature sensor, the temperature acquisition circuit is also connected with the MCU master control circuit, and the MCU master control circuit is connected with the storage device, the wireless transceiving circuit and the power management module.
9. The digital PCR instrument temperature testing device of claim 4, wherein: a temperature-sensitive probe shell is arranged in a limiting hole of the limiting structural member, and the temperature-sensitive probe shell is sleeved on the temperature-sensitive probe.
10. The digital PCR instrument temperature testing device of claim 4, wherein: the battery is installed on the circuit board, the shell is arranged at a disassembly opening for overhauling or disassembling the battery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221788582.0U CN217687590U (en) | 2022-07-12 | 2022-07-12 | Temperature-sensitive probe and temperature testing device of digital PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221788582.0U CN217687590U (en) | 2022-07-12 | 2022-07-12 | Temperature-sensitive probe and temperature testing device of digital PCR instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217687590U true CN217687590U (en) | 2022-10-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221788582.0U Active CN217687590U (en) | 2022-07-12 | 2022-07-12 | Temperature-sensitive probe and temperature testing device of digital PCR instrument |
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| Country | Link |
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| CN (1) | CN217687590U (en) |
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2022
- 2022-07-12 CN CN202221788582.0U patent/CN217687590U/en active Active
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