CN107941380A - A kind of PCR analyzer multichannel temperature calibrating installation - Google Patents
A kind of PCR analyzer multichannel temperature calibrating installation Download PDFInfo
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- CN107941380A CN107941380A CN201711363836.8A CN201711363836A CN107941380A CN 107941380 A CN107941380 A CN 107941380A CN 201711363836 A CN201711363836 A CN 201711363836A CN 107941380 A CN107941380 A CN 107941380A
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- 238000009434 installation Methods 0.000 title claims abstract description 18
- 238000012545 processing Methods 0.000 claims abstract description 33
- 238000009529 body temperature measurement Methods 0.000 claims abstract description 24
- 238000012937 correction Methods 0.000 claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 230000002687 intercalation Effects 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- UELITFHSCLAHKR-UHFFFAOYSA-N acibenzolar-S-methyl Chemical compound CSC(=O)C1=CC=CC2=C1SN=N2 UELITFHSCLAHKR-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K15/00—Testing or calibrating of thermometers
- G01K15/005—Calibration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K7/00—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements
- G01K7/16—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using resistive elements
- G01K7/22—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using resistive elements the element being a non-linear resistance, e.g. thermistor
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Nonlinear Science (AREA)
- Measuring Temperature Or Quantity Of Heat (AREA)
Abstract
The present invention relates to PCR analyzer temperature correction field, more particularly to a kind of PCR analyzer multichannel temperature calibrating installation, including multiple temperature sensors, the signal output part of the multiple temperature sensor is connected with a multichannel change-over switch, the multichannel change-over switch is connected with an integrated temperature measurement module, the integrated temperature measurement module is connected with a micro processing module, the output terminal of the micro processing module connects the control terminal of the multichannel change-over switch, the micro processing module is connected with a computer control unit, the computer control unit is used to show, storage information simultaneously generates Calibration Report；The temperature sensor includes 15 plug-in type thermistor (temperature) sensors and 1 pasted sheet type thermistor sensor；The micro processing module controls multichannel change-over switch by protocol signal control circuit；The integrated temperature measurement module is to compare temperature measurement circuit.
Description
Technical field
The present invention relates to PCR analyzer temperature correction field, more particularly to a kind of PCR
Analyzer multichannel temperature calibrating installation.
Background technology
PCR is that a kind of method of its amplification is realized in vitro enzyme' s catalysis specific DNA or RNA.
PCR is carried out by loop cycle, and each cycle includes high-temperature denatured, process annealing (renaturation) and thermophilic extends.Temperature
The stability and accuracy of degree directly affect the result of PCR.PCR analyzer can imitate
Bion, required temperature environment conditions are provided for PCR.PCR analyzer temperature control
Dynamic, steady-state characteristic, include the stability and uniformity of temperature, and temperature switching rate and maximum ultra-high temperature etc., directly
Influence the result of specific gene amplification.With the growth and frequently use of time of coming into operation, PCR analysis
The problems such as temperature control system of instrument, cooling system degradation, temperature sensor precise decreasing and heat-conducting glue local ageing, so that whole
Body reduces the service precision of instrument.Therefore, PCR analyzer is needed after regularly carrying out temperature measurement calibration
It could use.
The present invention proposes a kind of PCR analyzer temperature calibration instrument based on multichannel temperature sensor,
It can realize the on-line calibration of PCR analyzer multi-channel reaction test tube temperature, in addition, have also been devised patch
Chip heat-sensitive sensor is used for the on-line calibration of PCR analyzer head cover temperature；It is proposed using small, response
Fireballing thermistor as sensor, while propose temperature correction curve using thermistor to each thermistor into
Row calibration, improves thermometric accuracy.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of rational in infrastructure, quick response, accuracy of detection
A kind of high PCR analyzer multichannel temperature calibrating installation.
The present invention is achieved by the following technical solutions：
A kind of PCR analyzer multichannel temperature calibrating installation, it is characterised in that：Passed including multiple temperature
Sensor, the signal output part of the multiple temperature sensor are connected with a multichannel change-over switch, which connects
An integrated temperature measurement module is connected to, the integrated temperature measurement module is connected with a micro processing module, the micro processing module
Output terminal connects the control terminal of the multichannel change-over switch, and the micro processing module is connected with a computer control unit,
The computer control unit is used to show, stores information and generate Calibration Report.
Moreover, the temperature sensor includes 15 plug-in type thermistor (temperature) sensors and 1 pasted sheet type thermistor passes
Sensor.The plug-in type thermistor (temperature) sensor intercalation reaction test tube interior, the patch type heat-sensitive sensor are pasted onto circuit
Plate surface.
Moreover, the micro processing module controls multichannel change-over switch by protocol signal control circuit.
Moreover, the integrated temperature measurement module is to compare temperature measurement circuit.
Moreover, micro processing module is connected by USB general-purpose interfaces with computer control unit.
Moreover, the computer control unit includes computer control module, the computer control module and miniature processing
Module is connected, and the output terminal of the computer control module is connected to real-time display module and data memory module, should
The output terminal of data memory module is connected with Calibration Report generation module.
Moreover, computer control module is built-in with the temperature correction curve of each temperature sensor.
The advantages and positive effects of the present invention are：
1st, in this calibrating installation, multiple temperature sensors include 15 plug-in type thermistor (temperature) sensors and 1 patch type
Thermistor (temperature) sensor, the plug-in type thermistor (temperature) sensor are used to detect the temperature in reaction tube, and patch type temperature-sensitive passes
Sensor is then used for the temperature for detecting PCR analyzer head cover, and micro processing module passes through protocol signal control circuit
Multichannel change-over switch is controlled, it can be achieved that the information gathering and processing for temperature sensor of multichannel, integrated thermometric mould
Block coupled computer control unit is then used for realization carries out temperature correction for each temperature sensor；
2nd, in this calibrating installation, the integrated temperature measurement module passes through the ratio with measuring resistance to compare temperature measurement circuit
Compared with measurement, the thermometric resistance value of temperature sensor can be obtained, and is obtained by the temperature correction curve built in computer control module
Obtain the measurement temperature of temperature sensor；
3rd, in this calibrating installation, the computer control module of computer control unit is used for the letter for receiving micro processing module
Number, and computer control unit is controlled, display module is used for real-time display corresponding information, and data memory module is then used for
Corresponding data is stored, and corresponding information is exported to Calibration Report generation module, so as to carry out the generation of Calibration Report；
4th, in this calibrating installation, micro processing module is connected by USB general-purpose interfaces with computer control unit, lifting
Its versatility, connects easy to equipment and carries out input and output to corresponding data；
5th, in the present invention, the detection demand for new specification to PCR analyzer temperature-responsive speed, is adopted
By the use of the short time, quickly corresponding thermistor (temperature) sensor is carried out as temperature sensor, while for each thermistor (temperature) sensor
Temperature correction, improves the measurement accuracy of PCR analyzer reaction tube temperature；At the same time miniature place is utilized
The control system that module, protocol signal control circuit, multichannel change-over switch and integrated temperature measurement module are formed is managed, by several
The circulation of step, it is possible to achieve the information gathering of multichannel temperature sensor and processing function.
Brief description of the drawings
Fig. 1 is the module diagram of the present invention.
Embodiment
Elaborate below by embodiment to the present invention, the embodiment be it is illustrative and not restrictive, no
Protection scope of the present invention can be limited with this.
A kind of PCR analyzer multichannel temperature calibrating installation, innovation of the invention be, including multiple
Temperature sensor, the signal output part of the multiple temperature sensor are connected with a multichannel change-over switch, multichannel conversion
Switch is connected with an integrated temperature measurement module, and the integrated temperature measurement module is connected with a micro processing module, the miniature processing
The output terminal of module connects the control terminal of the multichannel change-over switch, and the micro processing module is connected with computer control
Unit, the computer control unit are used to show, store information and generate Calibration Report.
In the present embodiment, the temperature sensor includes 15 plug-in type thermistor (temperature) sensors and 1 patch type temperature-sensitive
Electric resistance sensor.The plug-in type thermistor (temperature) sensor intercalation reaction test tube interior, the patch type heat-sensitive sensor are pasted
In circuit board surface.
In the present embodiment, the micro processing module controls multichannel change-over switch by protocol signal control circuit.
In the present embodiment, the integrated temperature measurement module is to compare temperature measurement circuit.
In the present embodiment, micro processing module is connected by USB general-purpose interfaces with computer control unit.
In the present embodiment, the computer control unit includes computer control module, the computer control module with it is micro-
Type processing module is connected, and the output terminal of the computer control module is connected to real-time display module and data storage mould
Block, the output terminal of the data memory module are connected with Calibration Report generation module；Computer control module is built-in with each temperature
The temperature correction curve of sensor.
The use process of the present invention：
The present invention in use,
Step 1：Under the control of computer control module, micro processing module sends a control signal to protocol signal control
Control signal is communicated to multichannel change-over switch by circuit, protocol signal control circuit；
Step 2：Multichannel change-over switch receive protocol signal control circuit signal, will with certain all the way temperature sensor connect
The switch connect is opened；
Step 3：The temperature sensor signal that integrated temperature measurement module connects multichannel change-over switch in certain passage is surveyed
Amount, and signal is delivered to micro processing module；
Step 4：Micro processing module is by the detection signal output of integrated temperature measurement module to computer control system, at the same time
The control signal of (step 1) is sent to protocol signal control circuit, carries out the circulation of next round, it is continual successively to pass multichannel
Sensor detection signal is delivered to computer control system, is handled so as to fulfill the continuous acquisition of multichannel temperature information.
The design uses the plug-in type thermistor temperature sensor and pasted sheet type thermistor temperature of high-precision quick response
Sensor is spent as temperature sensor, and datum hole bottom is inserted into after encapsulation and carries out temperature online testing calibration.Integrated temperature measurement module
Using temp measuring method is compared, the actual resistance of tested sensor is relatively obtained by the magnitude of voltage with circuit Plays resistance.
The circuit system being made of multichannel change-over switch, integrated temperature measurement module, micro processing module and protocol signal control circuit
Realize the Signal acquiring and processing of multichannel temperature sensor, final micro processing module will detect signal and pass through the general strings of USB
Mouth is sent to computer control module, using the temperature correction curve of built-in each temperature sensor, obtains each temperature sensing
The actual measurement temperature of device, and the interface real-time display for passing through design.System will also automatically generate the calibration certificate of PDF format,
The printing of calibration certificate can be realized by external printer equipment.
Claims (7)
- A kind of 1. PCR analyzer multichannel temperature calibrating installation, it is characterised in that：Including multiple temperature sensing Device, the signal output part of the multiple temperature sensor are connected with a multichannel change-over switch, multichannel change-over switch connection Have an integrated temperature measurement module, the integrated temperature measurement module is connected with a micro processing module, the micro processing module it is defeated Outlet connects the control terminal of the multichannel change-over switch, and the micro processing module is connected with a computer control unit, should Computer control unit is used to show, stores information and generate Calibration Report.
- 2. a kind of PCR analyzer multichannel temperature calibrating installation according to claim 1, its feature exist In：The temperature sensor includes 15 plug-in type thermistor (temperature) sensors and 1 pasted sheet type thermistor sensor.
- 3. a kind of PCR analyzer multichannel temperature calibrating installation according to claim 1, its feature exist In：The micro processing module controls multichannel change-over switch by protocol signal control circuit.
- 4. a kind of PCR analyzer multichannel temperature calibrating installation according to claim 1, its feature exist In：The integrated temperature measurement module is to compare temperature measurement circuit.
- 5. a kind of PCR analyzer multichannel temperature calibrating installation according to claim 1, its feature exist In：Micro processing module is connected by USB general-purpose interfaces with computer control unit.
- 6. a kind of PCR analyzer multichannel temperature calibrating installation according to claim 1, its feature exist In：The computer control unit includes computer control module, which is connected with micro processing module, The output terminal of the computer control module is connected to real-time display module and data memory module, the data memory module Output terminal be connected with Calibration Report generation module.
- 7. a kind of PCR analyzer multichannel temperature calibrating installation according to claim 6, its feature exist In：Computer control module is built-in with the temperature correction curve of each temperature sensor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711363836.8A CN107941380A (en) | 2017-12-18 | 2017-12-18 | A kind of PCR analyzer multichannel temperature calibrating installation |
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| CN201711363836.8A CN107941380A (en) | 2017-12-18 | 2017-12-18 | A kind of PCR analyzer multichannel temperature calibrating installation |
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| CN107941380A true CN107941380A (en) | 2018-04-20 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109759162A (en) * | 2019-01-15 | 2019-05-17 | 北京市计量检测科学研究院 | Gene-amplificative instrament test stone device |
| CN111811676A (en) * | 2020-06-05 | 2020-10-23 | 北京空间飞行器总体设计部 | High-precision distributed temperature measurement system |
Citations (8)
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|---|---|---|---|---|
| US4031355A (en) * | 1975-03-31 | 1977-06-21 | Leonik Jr Stephen A | Multi-channel temperature responsive process control system |
| KR20020082022A (en) * | 2001-04-23 | 2002-10-30 | 삼성전자 주식회사 | Miniature gene analytical device using multichannel PCR and electrophoresis |
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2017
- 2017-12-18 CN CN201711363836.8A patent/CN107941380A/en active Pending
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109759162A (en) * | 2019-01-15 | 2019-05-17 | 北京市计量检测科学研究院 | Gene-amplificative instrament test stone device |
| CN111811676A (en) * | 2020-06-05 | 2020-10-23 | 北京空间飞行器总体设计部 | High-precision distributed temperature measurement system |
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