CN111504515A - Calibration device and calibration method for wireless transmission multi-channel PCR analyzer - Google Patents

Calibration device and calibration method for wireless transmission multi-channel PCR analyzer Download PDF

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Publication number
CN111504515A
CN111504515A CN202010504782.8A CN202010504782A CN111504515A CN 111504515 A CN111504515 A CN 111504515A CN 202010504782 A CN202010504782 A CN 202010504782A CN 111504515 A CN111504515 A CN 111504515A
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temperature
temperature sensor
calibration
data analysis
constant
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冯帅博
李博
胡博
贾会
许建军
袁晓丽
杨宇
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Henan Institute of Metrology
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Henan Institute of Metrology
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K15/00—Testing or calibrating of thermometers
    • G01K15/005—Calibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K7/00—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements
    • G01K7/16—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using resistive elements
    • G01K7/18—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using resistive elements the element being a linear resistance, e.g. platinum resistance thermometer
    • G—PHYSICS
    • G08—SIGNALLING
    • G08C—TRANSMISSION SYSTEMS FOR MEASURED VALUES, CONTROL OR SIMILAR SIGNALS
    • G08C17/00—Arrangements for transmitting signals characterised by the use of a wireless electrical link
    • G08C17/02—Arrangements for transmitting signals characterised by the use of a wireless electrical link using a radio link

Abstract

The invention relates to a calibration device and a calibration method of a wireless transmission multi-channel PCR analyzer, which adopt a wireless transmission technology to ensure that workers do not need to be in a high-risk environment for a long time, adopt a packaging structure to integrate a plurality of temperature sensors together to manufacture a multi-channel temperature sensor integrated device, and the packaging structure ensures that the temperature sensors can be immersed in a liquid heat-equalizing environment, and calibrate the temperature sensors of the calibration device by utilizing the liquid heat-equalizing environment, thereby realizing the quantity tracing and self calibration during calibration, eliminating and reducing the error of the temperature sensors and ensuring the accuracy and reliability of the calibration result. Therefore, the problems of magnitude traceability and self-calibration of the temperature sensor for calibrating the polymerase chain reaction analyzer are solved.

Description

Calibration device and calibration method for wireless transmission multi-channel PCR analyzer
Technical Field
The invention relates to the technical field of calibration and verification of polymerase chain reaction analyzers, in particular to a calibration device and a calibration method of a wireless transmission multi-channel PCR analyzer.
Background
The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the biggest characteristic of the PCR is that trace amount of DNA can be greatly increased. Therefore, even if only a single spot of DNA can be isolated from the hairs, skin or blood left by the murder in the murder case, or the remains of the historic creatures and the historical characters in the fossil, the DNA can be amplified by PCR and compared.
Semi-preserved replication of DNA is an important route for biological evolution and passage. Double-stranded DNA can be denatured and uncoiled into single strands under the action of various enzymes, and the double-stranded DNA is replicated into two identical molecular copies according to the base complementary pairing principle in the presence of DNA polymerase. DNA may also melt at high temperatures and may renature to double strands when the temperature is lowered. Therefore, the denaturation and renaturation of DNA are controlled by temperature change, and the in vitro replication of a specific gene can be completed by adding designed primers, DNA polymerase and dNTP. The Polymerase Chain Reaction (PCR) is to use the principle that DNA becomes single-stranded when denatured at 95 ℃ in vitro, and primer and single-stranded are combined according to the principle of base complementary pairing at low temperature (usually about 60 ℃), then the temperature is adjusted to the optimum reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes complementary strand along the direction from phosphoric acid to pentose (5 '-3'). Therefore, the PCR analyzer manufactured according to the PCR technology is actually a temperature control device, and can be well controlled among denaturation temperature, renaturation temperature and extension temperature, so as to rapidly simulate a specific temperature environment, thereby rapidly completing in vitro replication of a specific gene in biochemical analysis. Therefore, the accuracy of the temperature simulated by the PCR analyzer is a key parameter for quickly finishing in vitro replication of a specific gene, and is the basis for carrying out gene detection and analysis work, and the accuracy of the temperature directly influences the accuracy of gene analysis.
At present, with the development of gene technology, the application of the polymerase chain reaction analyzer is increasingly wide, statistics shows that the polymerase chain reaction analyzer is mainly applied to the fields of judicial investigation, medical clinical diagnosis, disease prevention, animal epidemic disease prevention, biomedical research and development, entry and exit inspection and quarantine and the like, but with the increase and frequent use of the polymerase chain reaction analyzer in use time, the problems of poor performance of a temperature control system and a heat dissipation system, reduced precision of a temperature sensor, local aging of heat-conducting glue and the like of the polymerase chain reaction analyzer are solved, so that the use precision of the analyzer is integrally reduced. Therefore, the PCR analyzer needs to be calibrated regularly for temperature measurement before it can be used.
At present, when a polymerase chain reaction analyzer is calibrated, a temperature sensor is mostly required to be manually inserted into a reaction test tube, the temperature of the reaction test tube is manually collected and recorded, and then items such as temperature indication errors and temperature uniformity are calibrated according to JJF1527-2015 polymerase chain reaction analyzer calibration standards.
In addition, in order to ensure the accuracy of the calibration result of the polymerase chain reaction analyzer, firstly, the accuracy of the adopted calibration equipment needs to be ensured, then, the temperature sensor needs to be calibrated regularly to perform quantity value tracing and self-calibration on the temperature sensor so as to ensure that the measurement error of the calibration equipment meets the use requirement, however, the soaking environment adopted by the existing polymerase chain reaction analyzer is a solid soaking environment, the existing temperature tracing system adopts a liquid soaking environment, and no corresponding solid soaking environment exists, so that the existing calibration method of the polymerase chain reaction analyzer lacks corresponding quantity value tracing and self-calibration steps, for the accuracy of the calibration result, the judgment can only depend on the experience of the staff according to the acquired temperature data, and the accuracy of the calibration result is poor.
Disclosure of Invention
In summary, in order to overcome the defects of the prior art, the invention provides a calibration device and a calibration method for a wireless transmission multichannel PCR analyzer, which adopt a wireless transmission technology to enable workers not to be in a high-risk environment for a long time, adopt a packaging structure to integrate a plurality of temperature sensors together to manufacture a multichannel temperature sensor integrated device, and enable the temperature sensors to be immersed in a liquid soaking environment through the packaging structure, thereby solving the problems of magnitude traceability and self calibration of the temperature sensors for calibrating the polymerase chain reaction analyzer.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a wireless transmission multi-channel PCR analyzer calibration apparatus, wherein: the calibration control device comprises a shell, a circuit board, an ADC conversion module, a controller, a power module, a touch display screen, a display module and a wireless transmission module, wherein the circuit board is packaged in the shell, the touch display screen is arranged at the upper end of the shell, the circuit board is provided with the ADC conversion module, the controller, the power module, the display module and the wireless transmission module, the ADC conversion module is electrically connected with the controller, and the ADC conversion module is electrically connected with the multichannel temperature sensor integrated device through a data signal line, the power module be connected with the controller electricity, power module provides the power for controller, ADC conversion module, controller, display module and wireless transmission module, power module pass through the battery of power cord coupling casing internal encapsulation, controller and display module electricity be connected, display module is connected with the touch display screen electricity, controller and wireless transmission module electricity be connected, wireless transmission module pass through wireless communication network and connect remote data analysis display device, remote data analysis display device be handheld PC terminal.
The technical scheme of the invention can also be realized as follows: the wireless transmission module of the calibration control device is electrically connected with a wireless antenna arranged outside the shell, the wireless antenna is connected with a wireless receiving and transmitting terminal through a wireless communication network, the wireless receiving and transmitting terminal is connected with the remote data analysis and display device through a data line, and the wireless communication network is a loRa wireless communication technology, a Bluetooth wireless communication technology or a zigbee wireless communication technology.
The technical scheme of the invention can also be realized as follows: the multichannel temperature sensor integrated device comprises a plurality of temperature sensors, a sealing cover, a heat-conducting protective cover, a base and a circuit board, wherein a plurality of through holes for inserting the temperature sensors are arranged on a bottom plate of the base, the lower end of the bottom plate is fixedly connected with the heat-conducting protective cover, the heat-conducting protective cover is covered on the through holes, the temperature sensors are inserted in the heat-conducting protective cover from the through holes on the bottom plate, a mounting groove for mounting the circuit board is arranged in the base, the temperature sensors are electrically connected with the circuit board, the upper end of the base is fixedly connected with the sealing cover, a sealing device is arranged between the lower end surface of the sealing cover and the upper end surface of the base, the sealing device seals a gap between the lower end surface of the sealing cover and the upper end surface of the base, a data signal wire connecting socket is arranged on the circuit board, the data signal line connecting socket is inserted in the through hole.
The technical scheme of the invention can also be realized as follows: the sealing device comprises a sealing groove arranged on the upper end face of the base, a sealing gasket arranged in the sealing groove, and a protrusion arranged on the lower end face of the sealing cover and corresponding to the sealing groove, wherein the protrusion is pressed into the sealing groove after the sealing cover is tightly connected with the base.
The technical scheme of the invention can also be realized as follows: the temperature sensor is a film platinum resistance temperature sensor.
The technical scheme of the invention can also be realized in such a way that the controller adopts an STM32F429IGT6 x L QFP176 single chip microcomputer, and the ADC conversion module adopts AD7124-8 BCPZ.
The technical scheme of the invention can also be realized as follows: the data signal line is a rubber-coated flat cable.
The calibration method for calibrating the PCR analyzer by using the calibration device for the wireless transmission multi-channel PCR analyzer comprises the following steps: the method comprises the following steps:
first, temperature sensor calibration of a multi-channel temperature sensor integrated device
(1) Device connection
Checking whether the appearances of the multichannel temperature sensor integrated device, the calibration control device, the wireless antenna, the wireless transceiving terminal and the remote data analysis display device are damaged or not, then connecting the multichannel temperature sensor integrated device with the calibration control device through a rubber-coated flat cable, inserting the wireless antenna into the calibration control device, and connecting the wireless transceiving terminal with the remote data analysis display device through a data line;
(2) setting standard heat equalizing environment
A constant-temperature water tank or a constant-temperature oil tank is adopted as a standard soaking environment;
(3) starting up and preheating
Starting a power switch of the calibration control device and a power switch of the remote data analysis display device, starting the remote data analysis display device, and entering a normal working state after starting for 1 minute;
(4) the multichannel temperature sensor integrated device is stably placed in a constant-temperature water tank or a constant-temperature oil tank;
(5) setting the output standard temperature value T of the constant temperature water tank or the constant temperature oil tank0Starting a constant-temperature water tank or a constant-temperature oil tank, and starting heating; when the temperature of the constant temperature water tank or the constant temperature oil tank reaches the set temperature T0And after the temperature is stabilized, the remote data analysis display device respectively reads and records the temperature of the plurality of temperature sensors at the set temperature of T0Resistance signal value R generated in time01~R0 nRemote, long distanceThe data analysis display device is based on the set temperature T0And establishing a plane coordinate system by the resistance signal value, wherein the X axis represents the set temperature, the Y axis represents the resistance signal value, and the corresponding coordinate of the plurality of temperature sensors on the plane coordinate system is (T)0，R01），（T0，R0 2）……（T0，R0 n）；
(6) Repeating the step (5), resetting the output standard temperature value of the constant-temperature water tank or the constant-temperature oil tank, reading and recording resistance signal values generated by the plurality of temperature sensors when the temperature is set by the remote data analysis and display device for a plurality of times respectively, enabling the same temperature sensor to correspond to a plurality of coordinates on a plane coordinate system, sequentially connecting the plurality of coordinates corresponding to the same temperature sensor on the plane coordinate system to form a temperature-resistance signal value calibration curve of the temperature sensor, and thus completing calibration of the temperature sensor of the multi-channel temperature sensor integrated device of the calibration device of the wireless transmission multi-channel PCR analyzer;
(7) after all temperature sensors on the multichannel temperature sensor integrated device are calibrated, closing the constant-temperature water tank or the constant-temperature oil tank, taking the multichannel temperature sensor integrated device out of the constant-temperature water tank or the constant-temperature oil tank after the temperature of the multichannel temperature sensor integrated device is cooled to a safe temperature, and closing the calibration control device and the remote data analysis display device;
second, calibrating the PCR analyzer
(1) Device connection
Connecting the multi-channel temperature sensor integrated device calibrated in the first step with a calibration control device through a rubber-coated flat cable, then opening a temperature control cavity of the detected polymerase chain reaction analyzer, stably placing the multi-channel temperature sensor integrated device in the temperature control cavity of the detected polymerase chain reaction analyzer, closing the temperature control cavity, positioning the multi-channel temperature sensor integrated device in the temperature control cavity, positioning the calibration control device outside the temperature control cavity, and opening a power switch of the calibration control device;
(2) an operator holds the remote data analysis display device by hand, is far away from the use space of the polymerase chain reaction analyzer to be detected, turns on a power switch, starts the remote data analysis display device, and enters a normal working state after preheating for 1 minute;
(3) starting the detected PCR analyzer, and setting the output standard temperature T of the temperature control cavityInstrument for measuring the shape of a human bodyThe temperature control cavity begins to be heated up, and when the temperature of the temperature control cavity displayed by the detected polymerase chain reaction analyzer reaches the set temperature TInstrument for measuring the shape of a human bodyAnd after the temperature is stabilized, starting a remote data analysis display device to acquire data, automatically acquiring and storing the measured temperature values of the temperature control cavities measured by all the temperature sensors of the multi-channel temperature sensor integrated device by the remote data analysis display device according to JJF1527-2015 'calibration standard for polymerase chain reaction analyzers',
when data is collected, the data collection time is set to be t, the collection times in the collection time t are set to be n, and the resistance signal value A measured by the same temperature sensor is collected by the remote data analysis display device in the unit time t1、A2……AnAnd automatically calculating the measured temperature of the temperature control cavity measured by the temperature sensor as x according to the resistance signal value and the temperature-resistance signal value calibration curve of the temperature sensor calibrated in the first step1、x2……xnRemote data analysis display device for measuring temperature x1、x2……xnPerforming data analysis, eliminating abnormal measurement temperature, and averaging the rest measurement temperatures, wherein the average value of the measurement temperatures is the output standard temperature value T of the temperature control cavity of the detected PCR analyzerInstrument for measuring the shape of a human bodyThe temperature sensor measures the measured temperature value x of the temperature control cavityFruit of Chinese wolfberry，
(4) Data computation
Calculating temperature indicating value errors, temperature uniformity and temperature rising and falling rates of the detected polymerase chain reaction analyzer according to the measured temperature values of the temperature control cavity of the detected polymerase chain reaction analyzer collected in the step (3) and JJF1527-2015 polymerase chain reaction analyzer calibration standard;
(5) and issuing a verification report, and collecting the multi-channel temperature sensor integrated device, the calibration control device and the remote data analysis display device to finish the verification and calibration work of the detected polymerase chain reaction analyzer.
In the first step, after the multichannel temperature sensor integrated device is placed in the constant-temperature water tank or the constant-temperature oil tank, the temperature sensor of the multichannel temperature sensor integrated device is positioned below the liquid level of the constant-temperature water tank or the constant-temperature oil tank, and the distance between the lower end of the temperature sensor and the liquid level of the constant-temperature water tank or the constant-temperature oil tank is more than or equal to 100 mm.
The technical scheme of the invention can also be realized in such a way that the remote data analysis display device measures the temperature x1、x2……xnWhen data analysis is performed and abnormal measured temperatures are eliminated, the reliability of the measured temperatures is judged first, the threshold value △ E is calculated by the formula (1),
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（1）
in the formula:
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-threshold value
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-the value of the temperature sensor which has the greatest difference from the average of all measured temperature values, ° c
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Average of all measured temperature values of the temperature sensor, ° c
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-the standard deviation of the temperature sensor;
when in use
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The measured temperature (x) of the temperature sensor collected in unit time t is less than or equal to 11、x2……xn) Credibility, abnormal measurement temperature does not need to be eliminated;
when in use
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1 hour, the measured temperature (x) of the temperature sensor collected in unit time t1、x2……xn) Suspicious, at the moment, the abnormal measurement temperature needs to be found and eliminated, and the abnormal measurement temperature is searched according to a formula (2);
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=
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（2）
in the formula:
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-the measured temperature value, deg.C, of a certain measurement of the temperature sensor
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-the number of acquisitions per unit time t of the temperature sensor
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-the standard deviation of the temperature sensor;
when in use
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While measuring the temperature
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Abnormality, measured temperature of the temperature sensor collected during the calculation unit time t (x1、x2……xn) At the average temperature of (2), rejecting the measured temperature
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。
The invention has the beneficial effects that:
1. the invention adopts a wireless transmission technology, so that workers do not need to be in a high-risk environment for a long time, a plurality of temperature sensors are integrated together by adopting a packaging structure to manufacture a multi-channel temperature sensor integrated device, and the temperature sensors can be immersed in a liquid soaking environment by adopting the packaging structure, so that the problems of magnitude traceability and self calibration of the temperature sensors for calibrating the polymerase chain reaction analyzer are solved.
2. The multichannel temperature sensor integrated device is provided with the sealing device and the heat-conducting protective cover, the sealing device and the heat-conducting protective cover completely seal the temperature sensor and the circuit board in the base and the sealing cover to realize packaging, so that the multichannel temperature sensor integrated device can be completely immersed in a constant-temperature water tank or a constant-temperature oil tank used in a liquid soaking environment to realize calibration and magnitude tracing of the temperature sensor.
3. The calibration method comprises the steps of firstly calibrating a temperature sensor of a calibration device, measuring the temperature of a standard isothermal environment by using the temperature sensor of the calibration device, establishing a plane coordinate system, forming a temperature-resistance signal value calibration curve of the temperature sensor by using a resistance signal value generated by the temperature sensor and a set temperature, taking the calibration curve as a standard curve measured by the temperature sensor when a polymerase chain reaction analyzer is calibrated, and when the polymerase chain reaction analyzer is calibrated, obtaining a temperature value corresponding to a resistance signal value on the calibration curve obtained by a temperature control cavity measured by the temperature sensor, namely a measured temperature value of the temperature control cavity measured by the temperature sensor.
4. When the polymerase chain reaction analyzer is calibrated, data analysis and abnormal measurement temperature elimination are introduced, data correction of the measurement temperature value acquired by the temperature sensor is realized, and the calibration precision of the polymerase chain reaction analyzer is improved.
5. The calibration method is simple, convenient to operate, high in calibration detection efficiency and accurate in result, and the value tracing and self-calibration during calibration are realized through calibration of the temperature sensor of the calibration device, so that the error of the temperature sensor is eliminated and reduced, and the accuracy and reliability of the calibration result are ensured.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural diagram of the multi-channel temperature sensor integrated device of the present invention;
FIG. 3 is a schematic diagram of the arrangement of temperature sensors in the multi-channel temperature sensor integrated device of the present invention;
FIG. 4 is a control schematic of the calibration control of the present invention;
FIG. 5 is a circuit diagram of the calibration control apparatus of the present invention;
FIG. 6 is a circuit diagram of an ADC conversion module of the calibration control device according to the present invention
Fig. 7 is a calibration curve of the temperature-resistance signal value of the temperature sensor RtA1 of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
As shown in fig. 1, 2, 3, 4, 5 and 6, a calibration device for a wireless transmission multichannel PCR analyzer includes a multichannel temperature sensor integrated device 1, a calibration control device 2 and a remote data analysis display device 5, the multichannel temperature sensor integrated device 1 is electrically connected to the calibration control device 2 through a data signal line, the data signal line is encapsulated, the calibration control device 2 and the remote data analysis display device 5 are connected to the multichannel temperature sensor integrated device 1 through a wireless communication network, the multichannel temperature sensor integrated device 1 includes 15 temperature sensors 13, a sealing cover 12, a heat-conductive shield 14, a base 11 and a circuit board 15, a bottom plate of the base 11 is provided with 15 through holes for inserting the temperature sensors 13, a lower end of the bottom plate is fixedly connected with the heat-conductive shield 14, the heat-conductive shield 14 is covered on the through holes, the temperature sensors 13 are inserted into the heat-conductive shield 14 from the bottom plate, the temperature sensors 13 are thin film resistor temperature sensors 13, the thin film resistor temperature sensors 13 are PT-35B-grade, a power supply module 11 is provided with a power supply module for mounting, a power supply module 15, a power supply module 14 is provided in the base 11, a power supply module 14, a wireless transmission module 14 is electrically connected to a wireless transmission module 14, a wireless transmission control module 14, a wireless transmission module 14 is electrically connected to a wireless transmission module 14, a wireless transmission control module 14, a wireless transmission control module 14, a wireless transmission module 14 is provided with a wireless transmission control module 14, a wireless transmission module 14 is provided with a wireless transmission module 14, a wireless transmission module 20, a wireless transmission module 14, a wireless transmission module 20, a wireless transmission module 14.
As shown in fig. 3, the distribution diagram of the 15 temperature sensors 13 of the multichannel temperature sensor integrated device 1 includes RtA1, RtD1, RtH1, RtA4, RtE4, RtH4, RtA7, RtD7, RtH7, RtA10, RtE10, RtH10, RtA12, RtD12, and RtH 12.
The calibration method for calibrating the PCR analyzer by using the calibration device for the wireless transmission multi-channel PCR analyzer comprises the following steps: the method comprises the following steps:
first, temperature sensor 13 calibration of a multi-channel temperature sensor integrated device
(1) Device connection
The method comprises the steps of checking whether the appearances of a multi-channel temperature sensor integrated device 1, a calibration control device 2, a wireless antenna 3, a wireless transceiving terminal 4 and a remote data analysis display device 5 are damaged or not, then connecting the multi-channel temperature sensor integrated device 1 with the calibration control device 2 through a rubber-coated flat cable, connecting the calibration control device 2 with an alternating current power supply through a power line, inserting the wireless antenna 3 into the calibration control device 2, and connecting the wireless transceiving terminal 4 with the remote data analysis display device 5 through a data line;
(2) setting standard heat equalizing environment
A constant-temperature oil groove is adopted as a standard soaking environment;
(3) starting up and preheating
Starting a power switch of the calibration control device 2 and a power switch of the remote data analysis display device 5, starting the remote data analysis display device 5, and entering a normal working state after starting for 1 minute;
(4) the multichannel temperature sensor integrated device 1 is stably placed in a constant-temperature oil tank; the temperature sensor is positioned below the liquid level of the constant temperature oil tank, and the distance between the lower end of the temperature sensor 13 of the multi-channel temperature sensor integrated device 1 and the liquid level of the constant temperature oil tank is 100 mm.
(5) Set the output standard temperature value T of the constant temperature oil groove0Starting the constant-temperature oil tank, and starting heating; when the temperature of the constant temperature water tank reaches the set temperature T0And after the temperature is stabilized, the remote data analysis display device 5 respectively reads and records the temperature of the plurality of temperature sensors 13 at the set temperature T0Resistance signal value R generated in time01~R0 nThe remote data analysis display device 5 analyzes the set temperature T0And the resistance signal value establishes a plane coordinate system, the X axis represents the set temperature, the Y axis represents the resistance signal value, and then the corresponding coordinate of the plurality of temperature sensors 13 on the plane coordinate system is (T)0，R01），（T0，R0 2）……（T0，R0 n）；
(6) Repeating the step (5), resetting the output standard temperature value of the constant-temperature oil tank, reading and recording resistance signal values generated by the plurality of temperature sensors 13 when the temperature is set by the remote data analysis and display device 5 for a plurality of times respectively, enabling the same temperature sensor 13 to correspond to a plurality of coordinates on a plane coordinate system, and sequentially connecting the plurality of coordinates corresponding to the same temperature sensor 13 on the plane coordinate system to form a temperature-resistance signal value calibration curve of the temperature sensor 13, so as to finish the calibration of the temperature sensor 13 of the multi-channel temperature sensor integrated device 1 of the calibration device of the wireless transmission multi-channel PCR analyzer;
(7) after all the temperature sensors 13 on the multichannel temperature sensor integrated device 1 are calibrated, closing the constant temperature oil tank, taking the multichannel temperature sensor integrated device 1 out of the constant temperature oil tank after the temperature of the multichannel temperature sensor integrated device is cooled to a safe temperature, and closing the calibration control device 2 and the remote data analysis display device 5;
in this embodiment, taking the calibration of the temperature sensor RtA1 as an example, the output standard temperature value of the constant temperature oil tank is set to be T0=10℃、T1=20℃、T2=30℃、T3=40℃、T4=50℃、T5=60℃、T6=70℃、T7=80℃、T8=90℃、T9=100℃、T10=110℃、T11=120 ℃, when the temperature sensor RtA1 detects the temperature of the constant temperature oil groove, the generated resistance signal values are R respectively01=104.30Ω、R11=108.03Ω、R21=111.72Ω、R31=115.24Ω、R41=119.48Ω、R51=122.84Ω、R61=126.85Ω、R71=131.28Ω、R81=134.36Ω、R91=137.98Ω、R101=142.69Ω、R111=145.76 Ω. The remote data analysis display device 5 uses the set temperature as an X axis and uses the resistance signal value as a Y axis to establish a planar coordinate system, and the coordinate points corresponding to the calibration of the temperature sensor RtA1 on the planar coordinate system at each time are (10 ℃, 104.30 Ω), (20 ℃, 108.03 Ω), (30 ℃, 111.72 Ω), (40 ℃, 115.24 Ω), (50 ℃, 119.48 Ω), (60 ℃, 122.84 Ω), (70 ℃, 126.85 Ω), (80 ℃, 131.28 Ω), (90 ℃, 134.36 Ω), (100 ℃, 137.98 Ω), (110 ℃, 142.69 Ω), (120 ℃, 145.76 Ω), and the coordinate points are sequentially connected on the planar coordinate system to form a temperature-resistance signal value curve of the temperature sensor RtA1, which is the temperature-resistance signal value calibration curve of the temperature sensor RtA1, as shown in fig. 7.
The establishment of the calibration curve of the temperature-resistance signal values of the rest 14 temperature sensors 13 of the multi-channel temperature sensor integrated device 1 of the invention is the same as that of the temperature sensor RtA 1.
When the invention is implemented in actual operation, an output standard temperature value T of the constant-temperature oil tank is set0Then, coordinate points of the 15 temperature sensors 13 at the output standard temperature value can be obtained simultaneously.
Second, calibrating the PCR analyzer
(1) Device connection
Connecting a multi-channel temperature sensor integrated device 1 of a calibrating device of the linear transmission multi-channel PCR analyzer calibrated in the first step with a calibrating control device 2 through an encapsulated flat cable, then opening a temperature control cavity of the polymerase chain reaction analyzer to be detected, stably placing the multi-channel temperature sensor integrated device 1 in the temperature control cavity of the polymerase chain reaction analyzer to be detected, closing the temperature control cavity, at the moment, positioning the multi-channel temperature sensor integrated device 1 in the temperature control cavity, positioning the calibrating control device 2 outside the temperature control cavity, and after connecting the calibrating control device 2 with a power supply, opening a power switch of the calibrating control device 2;
(2) an operator holds the remote data analysis display device 5 by hand, is far away from the use space of the polymerase chain reaction analyzer to be detected, opens a power switch of the remote data analysis display device 5, starts the remote data analysis display device 5, and enters a normal working state after preheating for 1 minute;
(3) starting the detected PCR analyzer, and setting the output standard temperature T of the temperature control cavityInstrument for measuring the shape of a human bodyThe temperature control cavity begins to be heated up, and when the temperature of the temperature control cavity displayed by the detected polymerase chain reaction analyzer reaches the set temperature TInstrument for measuring the shape of a human bodyAnd after stabilization, the remote data analysis and display device 5 is started to carry out data acquisition, the remote data analysis and display device 5 automatically acquires and stores the measured temperature values of the temperature control cavities measured by all the temperature sensors 13 of the multi-channel temperature sensor integrated device 1 according to JJF1527-2015 'calibration standard for polymerase chain reaction analyzer',
when data is collected, the data collection time is set as t, the number of times in the collection time t is set as n, and the remote data analysis display device 5 collects the resistance signal value A measured by the same temperature sensor 13 in the unit time t1、A2……AnAnd automatically calculating the measured temperature of the temperature control cavity measured by the temperature sensor 13 as x according to the resistance signal value and the temperature-resistance signal value calibration curve of the temperature sensor 13 calibrated in the first step1、x2……xnRemote data analysis display device 5 for measuring temperature x1、x2……xnPerforming data analysis and eliminating abnormal measurementAfter measuring the temperature, calculating the average value of the rest measured temperatures, wherein the average value of the measured temperatures is the output standard temperature value T of the temperature control cavity of the detected PCR analyzerInstrument for measuring the shape of a human bodyThe temperature sensor 13 measures the measured temperature value x of the temperature control cavityFruit of Chinese wolfberry。
Remote data analysis display device 5 pairs of measured temperatures x1、x2……xnWhen data analysis is performed and abnormal measured temperatures are eliminated, the reliability of the measured temperatures is judged first, the threshold value △ E is calculated by the formula (1),
Figure 919296DEST_PATH_IMAGE012
（1）
in the formula:
Figure 292508DEST_PATH_IMAGE002
-threshold value
Figure 156559DEST_PATH_IMAGE003
The value of the temperature sensor 13 that differs the greatest from the average of all measured temperature values, ° c
Figure 298828DEST_PATH_IMAGE004
Average of all measured temperature values of the temperature sensor 13, ° c
Figure 855711DEST_PATH_IMAGE005
The standard deviation of the temperature sensor 13;
when in use
Figure 716219DEST_PATH_IMAGE002
The measured temperature (x) of the temperature sensor 13 collected in unit time t is less than or equal to 11、x2……xn) Credibility, abnormal measurement temperature does not need to be eliminated;
when in use
Figure 118382DEST_PATH_IMAGE006
Figure 118382DEST_PATH_IMAGE006
1 hour, the measured temperature (x) of the temperature sensor 13 collected per unit time t1、x2……xn) Suspicious, at the moment, the abnormal measurement temperature needs to be found and eliminated, and the abnormal measurement temperature is searched according to a formula (2);
Figure 882201DEST_PATH_IMAGE013
=
Figure 609985DEST_PATH_IMAGE014
（2）
in the formula:
Figure 692211DEST_PATH_IMAGE009
the measured temperature value, deg.C, of a certain measurement of the temperature sensor 13
Figure 163644DEST_PATH_IMAGE010
The number of measurements of the temperature sensor 13
Figure 280504DEST_PATH_IMAGE005
The standard deviation of the temperature sensor 13;
when in use
Figure 710348DEST_PATH_IMAGE011
While measuring the temperature
Figure 420816DEST_PATH_IMAGE009
Abnormality, measured temperature (x) of the temperature sensor 13 collected in the calculation unit time t1、x2……xn) At the average temperature of (2), rejecting the measured temperature
Figure 554994DEST_PATH_IMAGE009
。
The present embodiment takes the measurement temperature value collection of the temperature sensor RtA1 of the multichannel temperature sensor integrated device 1 as an example,setting the output standard temperature value of the detected PCR analyzer as TInstrument for measuring the shape of a human body=30 ℃, when the temperature sensor RtA1 detects the temperature control cavity of the detected polymerase chain reaction analyzer, 15 resistance signal values are read within 1 minute, and are respectively A1=111.60Ω、A2=111.60Ω、A3=111.53Ω、A4=111.60Ω、A5=111.63Ω、A6=111.60Ω、A7=111.82Ω、A8=111.60Ω、A9=111.65Ω、A10=111.70Ω、A11=111.75Ω、A12=111.80Ω、A13=115.70Ω、A14=111.90Ω、A15=111.60Ω。
By the resistance signal value A1For example, =111.60 Ω, the method for calculating the measured temperature value according to the resistance signal value and the measured temperature value-resistance signal value calibration curve calibrated in the first step by the remote data analysis display device 5 is described as follows: 111.60 Ω exists between 108.03 Ω and 111.72 Ω, corresponding to a temperature value of between 20 ℃ and 30 ℃, then 111.60 Ω corresponds to a measured temperature value of:
x1=20℃ +（（111.6 – 108.03）/ （111.72 – 108.03））*（30 - 20）
x1= 20℃ + 9.675℃
x1= 29.675℃
then the corresponding 5 measured temperature values on the calibration curve of the measured temperature value-resistance signal value of the temperature sensor RtA1 are x1=29.675℃、x2=29.675℃、x3=29.485℃、x4=29.675℃、x5=29.756℃、x6=29.675℃、x7=30.271℃、x8=29.675℃、x9=29.810℃、x10=29.946℃、x11=30.081℃、x12=30.217℃、x13=40.786℃、x14=30.488℃、x15=29.810℃。
The measured temperature value of the temperature sensor RtA1 collected in 1 minute per unit time is (29.675 deg.C, 29.675 deg.C, 29.675 deg.C, 29.675 deg.C, 29.675 deg.C, 29.675 deg.C, 29.675 deg.C, 29.675 deg.C, 29.810 deg.C, 29.946 deg.C, 30.081 deg.C, 30.217 deg.C, 40.786 deg.C, 30.488 deg.C, 29 deg.C810 deg.C) according to the formula
Figure 231088DEST_PATH_IMAGE015
The standard deviation s of the temperature sensor RtA1 was calculated to be 2.831.
The threshold △ E is calculated according to equation (1),
Figure 566254DEST_PATH_IMAGE016
Figure 357493DEST_PATH_IMAGE017
Figure 170728DEST_PATH_IMAGE018
Figure 262181DEST_PATH_IMAGE006
1, the temperature sensor RtA1 is suspicious at the measurement temperature (29.675 ℃, 29.675 ℃, 29.675 ℃, 29.675 ℃, 29.675 ℃, 29.675 ℃, 29.675 ℃, 29.675 ℃, 29.810 ℃, 29.946 ℃, 30.081 ℃, 30.217 ℃, 40.786 ℃, 30.488 ℃ and 29.810 ℃) collected within 1 minute; the abnormal measured temperature values need to be rejected,
searching abnormal measurement temperature according to a formula (2);
Figure 33828DEST_PATH_IMAGE019
=
Figure 46783DEST_PATH_IMAGE020
（2）
according to the calculation of the formula (2), when the measured temperature value measured by the temperature sensor RtA1 is 40.786 ℃, the calculated value is
Figure 398130DEST_PATH_IMAGE021
If the measured temperature value is more than 1, the measured temperature value is 40.786 ℃ abnormal, and after the abnormal measured temperature is eliminated, the rest 14 measured temperatures are continuously judgedThe average value of the measured temperature is 30.1084 ℃, the measured value with the maximum difference value from the average value is 29.675 ℃, calculated
Figure 845554DEST_PATH_IMAGE022
1, the residual 14 temperature measurement results are credible, the average value of the 14 credible measurement temperature values is 30.1084 ℃, and the output standard temperature value of the detected polymerase chain reaction analyzer is set as TInstrument for measuring the shape of a human bodyTemperature sensor RtA1, measured temperature value x of temperature-controlled cavity measured at temperature of =30 ℃Fruit of Chinese wolfberry=30.1084℃。
Repeating the above operations, the remote data analysis display device 5 collects the output standard temperature value T of the temperature control cavity of the detected PCR analyzerInstrument for measuring the shape of a human bodyAnd when the temperature is not less than 30 ℃, the temperature values measured by the rest 14 temperature sensors 13 of the multi-channel temperature sensor integrated device 1 are measured.
Repeating the above operation, and respectively setting output standard temperature value T of the detected PCR analyzer according to JJF1527-2015 "calibration Standard of PCR analyzerInstrument for measuring the shape of a human bodyThe temperature values measured by the 15 temperature sensors 13 of the multi-channel temperature sensor integrated device 1 under different set temperature conditions are acquired by the remote data analysis display device 5 at 50 ℃, 60 ℃, 70 ℃, 90 ℃ and 95 ℃.
(4) Data computation
Calculating temperature indicating value errors, temperature uniformity and temperature rising and falling rates of the detected polymerase chain reaction analyzer according to the measured temperature values of the temperature control cavity of the detected polymerase chain reaction analyzer collected in the step (3) and JJF1527-2015 polymerase chain reaction analyzer calibration standard;
(5) and issuing a verification report, and collecting the multi-channel temperature sensor integrated device 1, the calibration control device 2 and the remote data analysis display device 5, so as to finish the verification and calibration work of the detected PCR analyzer.
It should be noted that the above-mentioned embodiments illustrate rather than limit the technical solutions of the present invention, and that equivalent substitutions or other modifications made by persons skilled in the art according to the prior art are included in the scope of the claims of the present invention as long as they do not exceed the spirit and scope of the technical solutions of the present invention.

Claims (9)

1. The utility model provides a wireless transmission multichannel PCR analysis appearance calibrating device which characterized in that: the device comprises a multi-channel temperature sensor integrated device (1), a calibration control device (2) and a remote data analysis display device (5), wherein the multi-channel temperature sensor integrated device (1) is electrically connected with the calibration control device (2) through a data signal line, the calibration control device (2) is connected with the remote data analysis display device (5) through a wireless communication network, the calibration control device (2) comprises a shell, a circuit board, an ADC (analog to digital converter) module (23), a controller (22), a power supply module (25), a touch display screen (21), a display module (24) and a wireless transmission module (26), the circuit board is packaged in the shell, the touch display screen (21) is arranged at the upper end of the shell, the ADC conversion module (23), the controller (22), the power supply module (25), the display module (24) and the wireless transmission module (26) are installed on the circuit board, the ADC conversion module (23) is electrically connected with the controller (22), the ADC conversion module (23) is electrically connected with the multichannel temperature sensor integrated device (1) through a data signal line, the power supply module (25) is electrically connected with the controller (22), the power supply module (25) provides power for the controller (22), the ADC conversion module (23), the controller (22), the display module (24) and the wireless transmission module (26), the power supply module (25) is connected with a battery packaged in the shell through a power line, the controller (22) is electrically connected with the display module (24), the display module (24) is electrically connected with the touch display screen (21), the controller (22) is electrically connected with the wireless transmission module (26), the wireless transmission module (26) is connected with the remote data analysis display device (5) through a wireless communication network, the remote data analysis display device (5) is a handheld PC terminal.
2. The wireless transmission multi-channel PCR analyzer calibration device according to claim 1, wherein: the calibration control device is characterized in that a wireless transmission module (26) of the calibration control device (2) is electrically connected with a wireless antenna (3) arranged outside the shell, the wireless antenna (3) is connected with a wireless receiving and transmitting terminal (4) through a wireless communication network, the wireless receiving and transmitting terminal (4) is connected with a remote data analysis display device (5) through a data line, and the wireless communication network is a loRa wireless communication technology, a Bluetooth wireless communication technology or a zigbee wireless communication technology.
3. The wireless transmission multi-channel PCR analyzer calibration device according to claim 1, wherein: the multichannel temperature sensor integrated device (1) comprises a plurality of temperature sensors (13), a sealing cover (12), a heat-conducting protective cover (14), a base (11) and a circuit board (15), wherein a plurality of through holes for inserting the temperature sensors (13) are formed in a bottom plate of the base (11), the lower end of the bottom plate is fixedly connected with the heat-conducting protective cover (14), the heat-conducting protective cover (14) is covered on the through holes, the temperature sensors (13) are inserted into the heat-conducting protective cover (14) from the through holes in the bottom plate, a mounting groove for mounting the circuit board (15) is formed in the base (11), the temperature sensors (13) are electrically connected with the circuit board (15), the upper end of the base (11) is fixedly connected with the sealing cover (12), and a sealing device is arranged between the lower end face of the sealing cover (12) and the upper end face of the base (11), the sealing device is characterized in that a gap is formed between the lower end face of the sealing cover (12) and the upper end face of the base (11), a data signal line connecting socket is arranged on the circuit board (15), a through hole is formed in one side of the base (11), and the data signal line connecting socket is inserted into the through hole.
4. The wireless transmission multi-channel PCR analyzer calibration device according to claim 1, wherein: the sealing device comprises a sealing groove (17) arranged on the upper end face of a base (11), a sealing gasket (18) arranged in the sealing groove (17), and a protrusion (16) which is arranged on the lower end face of a sealing cover (12) and corresponds to the sealing groove (17), wherein the protrusion (16) is pressed into the sealing groove (17) after the sealing cover (12) is tightly connected with the base (11).
5. The wireless transmission multi-channel PCR analyzer calibration device according to claim 1, wherein: the temperature sensor (13) is a thin film platinum resistance temperature sensor.
6. The calibration device of the wireless transmission multi-channel PCR analyzer according to claim 1, wherein the controller (22) adopts STM32F429IGT6 x L QFP176 single chip microcomputer, and the ADC conversion module (23) adopts AD7124-8 BCPZ.
7. The calibration method for calibrating a PCR analyzer by using the calibration device for a wireless transmission multi-channel PCR analyzer according to any one of claims 1 to 6, wherein the calibration method comprises the following steps: the method comprises the following steps:
firstly, calibrating a temperature sensor (13) of a multi-channel temperature sensor integrated device (1)
(1) Device connection
The method comprises the steps of checking whether the appearances of a multi-channel temperature sensor integrated device (1), a calibration control device (2), a wireless antenna (3), a wireless transceiving terminal (4) and a remote data analysis display device (5) are damaged or not, then connecting the multi-channel temperature sensor integrated device (1) with the calibration control device (2) through a rubber-coated flat cable, inserting the wireless antenna (3) into the calibration control device (2), and connecting the wireless transceiving terminal (4) with the remote data analysis display device (5) through a data line;
(2) setting standard heat equalizing environment
A constant-temperature water tank or a constant-temperature oil tank is adopted as a standard soaking environment;
(3) starting up and preheating
Starting a power switch of the calibration control device (2) and a power switch of the remote data analysis display device (5), starting the remote data analysis display device (5), and entering a normal working state after starting for 1 minute;
(4) the multichannel temperature sensor integrated device (1) is stably placed in a constant-temperature water tank or a constant-temperature oil tank;
(5) setting the output standard temperature value T of the constant temperature water tank or the constant temperature oil tank0Starting a constant-temperature water tank or a constant-temperature oil tank, and starting heating; when the temperature of the constant temperature water tank or the constant temperature oil tank reaches the set temperature T0And after the temperature is stabilized, the remote data analysis display device respectively reads and records the temperature of the plurality of temperature sensors at the set temperature of T0Resistance signal value R generated in time01~R0 nThe remote data analysis display device analyzes the set temperature T0And establishing a plane coordinate system by the resistance signal value, wherein the X axis represents the set temperature, the Y axis represents the resistance signal value, and the corresponding coordinate of the plurality of temperature sensors on the plane coordinate system is (T)0，R01），（T0，R0 2）……（T0，R0 n）；
(6) Repeating the step (5), resetting the output standard temperature value of the constant-temperature water tank or the constant-temperature oil tank, reading and recording resistance signal values generated by a plurality of temperature sensors (13) when the temperature is set for a plurality of times by the remote data analysis display device (5), enabling the same temperature sensor (13) to correspond to a plurality of coordinates on a plane coordinate system, sequentially connecting the corresponding coordinates of the same temperature sensor (13) on the plane coordinate system to form a temperature-resistance signal value calibration curve of the temperature sensor (13), and completing the calibration of the temperature sensor (13) of the multi-channel temperature sensor integrated device (1) of the calibration device of the wireless transmission multi-channel PCR analyzer;
(7) after all temperature sensors (13) on the multichannel temperature sensor integrated device (1) are calibrated, closing the constant-temperature water tank or the constant-temperature oil tank, taking the multichannel temperature sensor integrated device (1) out of the constant-temperature water tank or the constant-temperature oil tank after the temperature of the multichannel temperature sensor integrated device is cooled to a safe temperature, and closing the calibration control device (2) and the remote data analysis display device (5);
second, calibrating the PCR analyzer
(1) Device connection
Connecting the multi-channel temperature sensor integrated device (1) calibrated in the first step with a calibration control device (2) through a rubber-coated flat cable, then opening a temperature control cavity of the detected polymerase chain reaction analyzer, stably placing the multi-channel temperature sensor integrated device (1) in the temperature control cavity of the detected polymerase chain reaction analyzer, closing the temperature control cavity, positioning the multi-channel temperature sensor integrated device (1) in the temperature control cavity, positioning the calibration control device (2) outside the temperature control cavity, and opening a power switch of the calibration control device (2);
(2) an operator holds the remote data analysis display device (5), keeps away from the use space of the polymerase chain reaction analyzer to be detected, turns on a power switch, starts the remote data analysis display device (5), and enters a normal working state after preheating for 1 minute;
(3) starting the detected PCR analyzer, and setting the output standard temperature T of the temperature control cavityInstrument for measuring the shape of a human bodyThe temperature control cavity begins to be heated up, and when the temperature of the temperature control cavity displayed by the detected polymerase chain reaction analyzer reaches the set temperature TInstrument for measuring the shape of a human bodyAnd after the temperature is stabilized, starting a remote data analysis display device (5) to acquire data, automatically acquiring and storing the measured temperature values of the temperature control cavities measured by all the temperature sensors (13) of the multi-channel temperature sensor integrated device (1) by the remote data analysis display device (5) according to JJF1527-2015 'calibration standard for polymerase chain reaction analyzers',
when data are collected, the data collection time is set to be t, the number of times in the collection time t is set to be n, and the remote data analysis display device (5) collects the resistance signal value A measured by the same temperature sensor (13) in the unit time t1、A2……AnAnd automatically calculating the measured temperature of the temperature control cavity measured by the temperature sensor (13) as x according to the resistance signal value and the temperature-resistance signal value calibration curve of the temperature sensor (13) calibrated in the first step1、x2……xnThe remote data analysis display device (5) analyzes the measured temperatureDegree x1、x2……xnPerforming data analysis, eliminating abnormal measurement temperature, and averaging the rest measurement temperatures, wherein the average value of the measurement temperatures is the output standard temperature value T of the temperature control cavity of the detected PCR analyzerInstrument for measuring the shape of a human bodyThe temperature sensor (13) measures the measured temperature value x of the temperature control cavityFruit of Chinese wolfberry，
(4) Data computation
Calculating temperature indicating value errors, temperature uniformity and temperature rising and falling rates of the detected polymerase chain reaction analyzer according to the measured temperature values of the temperature control cavity of the detected polymerase chain reaction analyzer collected in the step (3) and JJF1527-2015 polymerase chain reaction analyzer calibration standard;
(5) and issuing a verification report, and collecting the multi-channel temperature sensor integrated device (1), the calibration control device (2) and the remote data analysis display device (5) so as to finish the verification and calibration work of the polymerase chain reaction analyzer to be detected.
8. The method for calibrating a PCR analyzer according to claim 7, wherein: in the first step, in the step (4) of calibrating the temperature sensor (13) of the calibrating device, after the multichannel temperature sensor integrated device (1) is placed in a constant-temperature water tank or a constant-temperature oil tank, the temperature sensor (13) is positioned below the liquid level of the constant-temperature water tank or the constant-temperature oil tank, and the distance between the lower end of the temperature sensor (13) and the liquid level of the constant-temperature water tank or the constant-temperature oil tank is more than or equal to 100 mm.
9. The method for calibrating a PCR analyzer according to claim 7, wherein: in the second step, in the step (3) of calibrating the polymerase chain reaction analyzer to be tested, the remote data analysis display device (5) is used for measuring the temperature x1、x2……xnWhen data analysis is performed and abnormal measured temperatures are eliminated, the reliability of the measured temperatures is judged first, the threshold value △ E is calculated by the formula (1),
Figure 9584DEST_PATH_IMAGE001
（1）
in the formula:
Figure 509835DEST_PATH_IMAGE002
-threshold value
Figure 664873DEST_PATH_IMAGE003
-the value of the temperature sensor which has the greatest difference from the average of all measured temperature values, ° c
Figure 465339DEST_PATH_IMAGE004
Average of all measured temperature values of the temperature sensor, ° c
Figure 844367DEST_PATH_IMAGE005
-the standard deviation of the temperature sensor;
when in use
Figure 238440DEST_PATH_IMAGE002
The measured temperature (x) of the temperature sensor (13) collected in unit time t is less than or equal to 11、x2……xn) Credibility, abnormal measurement temperature does not need to be eliminated;
when in use
Figure 56223DEST_PATH_IMAGE006
1 hour, the measured temperature (x) of the temperature sensor (13) collected in unit time t1、x2……xn) Suspicious, at the moment, the abnormal measurement temperature needs to be found and eliminated, and the abnormal measurement temperature is searched according to a formula (2);
Figure 852141DEST_PATH_IMAGE007
=
Figure 965852DEST_PATH_IMAGE008
（2）
in the formula:
Figure 581641DEST_PATH_IMAGE009
-the measured temperature value, deg.C, of a certain measurement of the temperature sensor
Figure 203116DEST_PATH_IMAGE010
The number of measurements of the temperature sensor
Figure 853540DEST_PATH_IMAGE005
-the standard deviation of the temperature sensor;
when in use
Figure 167847DEST_PATH_IMAGE011
While measuring the temperature
Figure 270932DEST_PATH_IMAGE009
An abnormality, a measured temperature (x) of the temperature sensor (13) acquired within a calculation unit time t1、x2……xn) At the average temperature of (2), rejecting the measured temperature
Figure 430518DEST_PATH_IMAGE009
。
CN202010504782.8A 2020-06-05 2020-06-05 Calibration device and calibration method for wireless transmission multi-channel PCR analyzer Pending CN111504515A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112945420A (en) * 2021-02-02 2021-06-11 四川淯源仪器科技有限公司 Temperature calibration system of wireless PCR instrument
CN113050719A (en) * 2021-01-12 2021-06-29 无锡市疾病预防控制中心 Experimental apparatus accuse temperature early warning system
CN113377140A (en) * 2021-06-09 2021-09-10 厦门大学 Temperature control method and device for nucleic acid detection device
CN115031871A (en) * 2022-08-11 2022-09-09 北京林电伟业电子技术有限公司 Multi-temperature-zone calibration method for constant-temperature rapid nucleic acid amplification detection analyzer

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113050719A (en) * 2021-01-12 2021-06-29 无锡市疾病预防控制中心 Experimental apparatus accuse temperature early warning system
CN112945420A (en) * 2021-02-02 2021-06-11 四川淯源仪器科技有限公司 Temperature calibration system of wireless PCR instrument
CN113377140A (en) * 2021-06-09 2021-09-10 厦门大学 Temperature control method and device for nucleic acid detection device
CN113377140B (en) * 2021-06-09 2022-10-04 厦门大学 Temperature control method and device for nucleic acid detection device
CN115031871A (en) * 2022-08-11 2022-09-09 北京林电伟业电子技术有限公司 Multi-temperature-zone calibration method for constant-temperature rapid nucleic acid amplification detection analyzer

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