CN217578932U - Cell culture plate convenient to rifle head location - Google Patents

Cell culture plate convenient to rifle head location Download PDF

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Publication number
CN217578932U
CN217578932U CN202221272899.9U CN202221272899U CN217578932U CN 217578932 U CN217578932 U CN 217578932U CN 202221272899 U CN202221272899 U CN 202221272899U CN 217578932 U CN217578932 U CN 217578932U
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plate body
culture plate
base
cell culture
sloping block
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CN202221272899.9U
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Inventor
李艳伟
邢月婷
黄莹莹
王佳佳
郭春
阿依西布·萨吾提
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Zhejiang University ZJU
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Zhejiang University ZJU
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Abstract

The utility model discloses a cell culture plate convenient to rifle head location relates to cell culture plate technical field. The utility model discloses a cultivate the plate body, cultivate the top of plate body and evenly seted up the cultivation hole, annular positioning groove has still been seted up on the inner wall of cultivation hole lower part, cultivate the top of plate body and still fixedly connected with vertical level bar and horizontal level bar, and the bottom of cultivateing the plate body still is provided with the arc connecting seat, the below of cultivateing the plate body is provided with the base, be provided with adjustment mechanism between cultivation plate body and the base, adjustment mechanism is including connecting the bulb, the screw rod, lower sloping block and last sloping block, it passes through bracing piece fixed connection in the base top to connect the bulb, the screw rod passes through the bearing and rotates the connection on the base. The utility model discloses an open in the cultivation hole at cultivation board plate body top and establish annular positioning groove, can fix a position the position of rifle head through annular positioning groove for inhale the even that the surplus culture solution in every cultivation hole after abandoning the liquid can be more.

Description

Cell culture plate convenient to rifle head location
Technical Field
The utility model belongs to the technical field of the cell culture board, especially, relate to a cell culture board convenient to rifle head location.
Background
Cell culture plates, as a means for cell culture, are widely used in cell research, including tumor cell research. Most tumor cells grow adherent to the wall, and when the activity of the tumor cells is researched by researching the inhibition of the drug on the tumor cells, the drug concentration and the action time are required to be searched to judge the action of the drug on the tumor cells. The experiments were performed with 96-well plates, and to avoid damage to the cells and evaporation problems when the medium was aspirated away, the wells were kept sufficiently filled with residual medium (20. Mu.L).
However, in actual operation, either the lance head enters too deeply to cause cell damage, or the lance head enters too shallowly to cause too much volume of the residual culture solution, so that the final residual volume of each hole is not uniform, and during subsequent dosing, the concentration difference of the drug is large, thereby influencing the judgment of the drug concentration for inhibiting the tumor activity.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model discloses a realize through following technical scheme:
the utility model relates to a cell culture plate convenient for positioning a gun head, which comprises a culture plate body, wherein culture holes are uniformly formed in the top of the culture plate body, annular positioning grooves are also formed in the inner wall of the lower part of each culture hole, the top of the culture plate body is also fixedly connected with a vertical horizontal ruler and a transverse horizontal ruler, the bottom of the culture plate body is also provided with an arc-shaped connecting seat, a base is arranged below the culture plate body, and an adjusting mechanism is arranged between the culture plate body and the base;
adjustment mechanism is including connecting bulb, screw rod, lower sloping block and going up the sloping block, it passes through bracing piece fixed connection in the base top to connect the bulb, the screw rod passes through the bearing and rotates to be connected on the base, and four sides of base all rotate and be connected with the screw rod, the sloping block cup joints on the screw rod down, go up sloping block fixed connection in the bottom of culture plate body, and the lower terminal surface laminating of the lower terminal surface of going up the sloping block and sloping block down.
Preferably, the lower end of the supporting rod is fixedly connected with the center of the top of the base, an arc-shaped connecting seat is arranged at the center of the bottom of the plate body of the culture plate, and the arc-shaped connecting seat is sleeved outside the connecting ball head.
Preferably, four upper inclined blocks are arranged at the bottom of the plate body of the culture plate, and the four upper inclined blocks are respectively positioned right above the four lower inclined blocks.
Preferably, the upper end surface of the lower inclined block is obliquely arranged, and the lower end surface of the upper inclined block is also obliquely arranged.
Preferably, one end of the screw rod is also provided with an adjusting head, and the peripheral side wall of the adjusting head is also provided with an anti-skid tooth groove.
Preferably, the culture holes on the culture plate body are arranged near the upper left of the culture plate body, and the culture holes on the culture plate body are arranged in a rectangular array.
Preferably, the vertical level is arranged along the vertical direction of the culture plate body, and the horizontal level is arranged along the horizontal direction of the culture plate body.
The utility model discloses following beneficial effect has:
1. the utility model discloses an open and establish annular positioning groove in the cultivation hole at cultivation board plate top, can fix a position the position of rifle head through annular positioning groove, can avoid the rifle head to get into too deeply and lead to the cell damage, can avoid the rifle head to get into too shallowly simultaneously for remaining liquid volume in the cultivation hole is too much, makes the culture solution that inhales the surplus in every cultivation hole after abandoning the liquid can be more even, makes the experiment can be more accurate.
2. The utility model discloses a fixed vertical level bar and horizontal level bar in the top of cultivateing the board plate body to set up adjustment mechanism in the below of cultivateing the board plate body, through the cooperation of connecting bulb, screw rod, lower sloping block and last sloping block, make the device when using, can adjust the horizontality of cultivateing the board plate body, can reduce the influence that cultivates the board plate body position slope and cause to the surplus culture solution volume after the liquid is abandoned to cultivateing in the hole, can further improve the precision of experiment.
Of course, it is not necessary for any particular product to achieve all of the above-described advantages simultaneously.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the description below are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a cross-sectional view of a cell culture plate of the present invention for facilitating positioning of a pipette tip;
FIG. 2 is an enlarged view of the structure A of FIG. 1 of a cell culture plate for facilitating positioning of a tip of a pipette tip according to the present invention;
fig. 3 is a top view of the cell culture plate convenient for positioning the gun head of the utility model.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a plate body of the culture plate; 11. a culture well; 12. an arc-shaped connecting seat; 13. an annular positioning groove; 2. a base; 21. a support bar; 3. connecting a ball head; 4. a screw; 41. an adjustment head; 5. a lower inclined block; 6. an upper inclined block; 7. a vertical level bar; 8. a transverse level.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1-3, the utility model relates to a cell culture plate convenient for positioning of a gun head, which comprises a culture plate body 1, wherein culture holes 11 are uniformly formed in the top of the culture plate body 1, an annular positioning groove 13 is further formed in the inner wall of the lower part of each culture hole 11, the annular positioning groove 13 is used for positioning the gun head, the gun head can be prevented from entering too deep to cause cell damage, and meanwhile, the gun head can be prevented from entering too shallow to cause excessive residual culture liquid in each culture hole 11, the annular positioning groove 13 is positioned at the position of 20 μ L in the culture plate body 1, the top of the culture plate body 1 is fixedly connected with a vertical horizontal ruler 7 and a horizontal ruler 8, an arc-shaped connecting seat 12 is further arranged at the bottom of the culture plate body 1, a base 2 is arranged below the culture plate body 1, and an adjusting mechanism is arranged between the culture plate body 1 and the base 2;
adjustment mechanism is including connecting bulb 3, screw rod 4, down sloping block 5 and last sloping block 6, it passes through bracing piece 21 fixed connection in base 2 top to connect bulb 3, screw rod 4 passes through the bearing and rotates the connection on base 2, and four sides of base 2 all rotate and be connected with screw rod 4, sloping block 5 cup joints on screw rod 4 down, it is in the bottom of cultivateing plate body 1 to go up 6 fixed connection of sloping block, and the lower terminal surface of going up sloping block 6 and the lower terminal surface laminating of sloping block 5 down, through the adjustment mechanism who sets up, can assist the personnel to adjust the horizontality of cultivateing plate body 1.
Further, the lower extreme of bracing piece 21 and the central point at base 2 top put fixed connection, and the central point department of putting of 1 bottom of cultivation board plate body is provided with arc connecting seat 12, and arc connecting seat 12 cover is established in the outside of connecting bulb 3, through arc connecting seat 12, bracing piece 21 and the connection bulb 3 that sets up for realize the swing joint of cultivation board plate body 1 and base 2, and can realize the smooth adjustment to 1 roughness of cultivation board plate body.
Further, the last sloping block 6 of 1 bottom of culture plate body is provided with four, four go up sloping block 6 and be located four sloping block 5 directly over respectively, the slope setting is personally submitted to the upper end of sloping block 5 down, the lower terminal surface of going up sloping block 6 also is the slope setting, the high inboard of the up end outside of sloping block 5 down is low, it is high to go up the bottom of the lower extreme outside of sloping block 6, sloping block 5 inwards moves down, relative sliding takes place with sloping block 5 down, can drive culture plate body 1 and rotate round connecting bulb 3, adjust culture plate body 1's horizontality.
Furthermore, one end of the screw rod 4 is also provided with an adjusting head 41, an anti-skid tooth groove is further formed in the peripheral side wall of the adjusting head 41, the adjusting head 41 can be manually stirred to drive the screw rod 4 to rotate, and then the lower inclined block 5 can be driven to move.
Further, cultivate the upper left side that hole 11 on the culture plate body 1 is close to culture plate body 1 and set up, and cultivate the hole 11 on the culture plate body 1 and be rectangular array and set up, vertical level 7 along the vertical setting of culture plate body 1, horizontal level 8 along the horizontal setting of culture plate body 1, can assist the personnel to judge the level degree of culture plate body 1 through vertical level 7 and horizontal level 8 that set up.
When using this culture plate body 1 to cultivate the cell, at first place the base 2 steady in suitable position department, judge the horizontal degree of culture plate body 1 through observing vertical level 7 and horizontal level 8, when the horizontal degree of culture plate body 1 needs to be adjusted, the position of four lower sloping blocks 5 is adjusted in personnel's accessible cooperation, make culture plate body 1 carry out the rotation of certain degree round connecting ball head 3, and make culture plate body 1 finally reach the level, when adjusting the position of sloping block 5 down, the accessible is manual to be stirred adjusting head 41 and is driven screw rod 4 and rotate, screw rod 4 rotates and can drive down sloping block 5 and remove, after the level of treating culture plate body 1 adjustment, can cultivate the cell through culture hole 11 on the culture plate body 1, when carrying out imbibition or abandoning the liquid to culture hole 11, can support the lower extreme of rifle head in the annular positioning groove 13 position department in culture hole 11, carry out imbibition or abandon the liquid operation to the inside of culture hole 11, can avoid getting into too deep cell damage, simultaneously can avoid getting into too shallow culture hole 11, make the surplus volume of culture hole 11 in the culture hole too much liquid.
In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above is only the preferred embodiment of the present invention, and the present invention is not limited thereto, and any technical solutions recorded in the foregoing embodiments are modified, and some technical features are replaced equally, all belonging to the protection scope of the present invention.

Claims (7)

1. The utility model provides a cell culture board convenient to rifle head location, includes culture plate body (1), culture hole (11), its characterized in that have evenly been seted up at the top of culture plate body (1): an annular positioning groove (13) is further formed in the inner wall of the lower portion of the culture hole (11), the top of the culture plate body (1) is fixedly connected with a vertical horizontal ruler (7) and a transverse horizontal ruler (8), an arc-shaped connecting seat (12) is further arranged at the bottom of the culture plate body (1), a base (2) is arranged below the culture plate body (1), and an adjusting mechanism is arranged between the culture plate body (1) and the base (2);
adjustment mechanism is including connecting bulb (3), screw rod (4), down sloping block (5) and last sloping block (6), it passes through bracing piece (21) fixed connection in base (2) top to connect bulb (3), screw rod (4) rotate through the bearing and connect on base (2), and four sides of base (2) all rotate and be connected with screw rod (4), sloping block (5) cup joint on screw rod (4) down, go up sloping block (6) fixed connection in the bottom of cultivateing plate body (1), and go up the lower terminal surface of sloping block (6) and the lower terminal surface laminating of sloping block (5) down.
2. The cell culture plate convenient for positioning the gun head according to claim 1, wherein the lower end of the support rod (21) is fixedly connected with the central position of the top of the base (2), an arc-shaped connecting seat (12) is arranged at the central position of the bottom of the culture plate body (1), and the arc-shaped connecting seat (12) is sleeved outside the connecting ball head (3).
3. The cell culture plate convenient for positioning the gun head according to claim 2, wherein four upper inclined blocks (6) are arranged at the bottom of the culture plate body (1), and the four upper inclined blocks (6) are respectively positioned right above the four lower inclined blocks (5).
4. A cell culture plate for facilitating the positioning of a lance tip according to claim 3, wherein the upper end surface of the lower inclined block (5) is inclined, and the lower end surface of the upper inclined block (6) is also inclined.
5. The cell culture plate convenient for positioning the lance head according to claim 4, wherein one end of the screw (4) is further provided with an adjusting head (41), and the peripheral side wall of the adjusting head (41) is further provided with an anti-slip tooth groove.
6. The cell culture plate convenient for positioning the lance head according to claim 1, wherein the culture holes (11) in the culture plate body (1) are formed near the upper left of the culture plate body (1), and the culture holes (11) in the culture plate body (1) are formed in a rectangular array.
7. The cell culture plate for facilitating positioning of the lance tip according to claim 1, wherein the vertical level (7) is arranged vertically along the plate body (1) of the culture plate, and the horizontal level (8) is arranged horizontally along the plate body (1) of the culture plate.
CN202221272899.9U 2022-05-25 2022-05-25 Cell culture plate convenient to rifle head location Active CN217578932U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202221272899.9U CN217578932U (en) 2022-05-25 2022-05-25 Cell culture plate convenient to rifle head location

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202221272899.9U CN217578932U (en) 2022-05-25 2022-05-25 Cell culture plate convenient to rifle head location

Publications (1)

Publication Number Publication Date
CN217578932U true CN217578932U (en) 2022-10-14

Family

ID=83554391

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202221272899.9U Active CN217578932U (en) 2022-05-25 2022-05-25 Cell culture plate convenient to rifle head location

Country Status (1)

Country Link
CN (1) CN217578932U (en)

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