CN217549842U

CN217549842U - Micro-fluidic chip for single cell sorting

Info

Publication number: CN217549842U
Application number: CN202221169711.8U
Authority: CN
Inventors: 贾广帅
Original assignee: Suzhou Quantitative Cell Biotechnology Co ltd
Current assignee: Suzhou Quantitative Cell Biotechnology Co ltd
Priority date: 2022-05-16
Filing date: 2022-05-16
Publication date: 2022-10-11
Anticipated expiration: 2032-05-16

Abstract

The utility model discloses a micro-fluidic chip for unicellular is selected separately, including last lamellar body and the lower lamellar body of laminating in last lamellar body bottom, go up the lamellar body and be connected with the fixer down between the periphery of lamellar body, the top of going up the lamellar body is constructed and is advanced the sample groove, the bottom of lamellar body is constructed and is appeared the sample groove down, the circulation pipeline that has multichannel parallel arrangement is constructed between the bottom of going up the lamellar body and the top of lower lamellar body, be connected with parallel arrangement's connecting tube between the circulation pipeline, the circulation pipeline is formed by the recess of last lamellar body bottom and the recess laminating at lower lamellar body top, the lateral wall of lamellar body is constructed and is had a plurality of collection mouths down, and the quantity of collecting the mouth is corresponding with the quantity of circulation pipeline. The cell sorting machine can effectively relieve the congestion condition when cells flow, can accommodate more cells, improves the cell sorting efficiency, is convenient to mount and dismount, and is convenient to use.

Description

Micro-fluidic chip for single cell sorting

Technical Field

The utility model relates to a biological sample handles technical field, in particular to a micro-fluidic chip for unicellular separation.

Background

Microfluidic technology, also known as lab-on-a-chip, can integrate conventional biochemical reactions into a few square centimeters chip. Due to the micron-sized channels of the microfluidic chip, which are equivalent to the size of the cells, the microfluidic chip has attracted more and more attention in the research of single cells.

The first microfluidic chip used for single cell research was the "Quake" chip, which was integrated with a complex pump-valve system to manipulate single cells. Later, with the development of droplet technology, drop-seq technology appeared, which greatly improves the throughput of single cell sequencing.

The existing microfluidic chip for single cell sorting is easy to block in the cell flowing process, is difficult to realize subsequent single cell control and analysis, and has lower flux.

To this end, we propose a microfluidic chip for single cell sorting.

SUMMERY OF THE UTILITY MODEL

The main object of the utility model is to provide a micro-fluidic chip for unicellular separation can effectively solve the problem among the background art.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a micro-fluidic chip for unicell is selected separately, includes last lamellar body and the lower lamellar body of laminating in last lamellar body bottom, go up the lamellar body and be connected with the fixer between the periphery of lamellar body down, the top of going up the lamellar body is constructed and is advanced kind of groove, the bottom of lamellar body is constructed and is advanced kind of groove down, go up the bottom of lamellar body and construct multichannel parallel arrangement's circulation pipeline down between the top of lamellar body, be connected with parallel arrangement's connecting tube between the circulation pipeline, the circulation pipeline is formed by the recess of last lamellar body bottom and the recess laminating at lower lamellar body top, pours the cell into back in advancing kind of groove along with the buffer solution into, and the cell flows along with the buffer solution in the circulation pipeline, when producing in the single circulation pipeline and blockking up, the cell can get into in the adjacent circulation pipeline through the connecting tube to alleviate the jam condition.

Furthermore, the side wall of the lower sheet body is provided with a plurality of collecting ports, the number of the collecting ports corresponds to the number of the flow pipelines, and the sampling cells are collected from the collecting ports.

Further, the fixer includes grip block, diaphragm, thread groove and bolt, the grip block laminating is at the lateral wall of last lamellar body and lower lamellar body, and the both ends of grip block all are connected with the diaphragm through integrated into one piece, the surface structure of diaphragm has the thread groove, and wears to be equipped with in the thread groove with last lamellar body and the bolt of lamellar body butt down, under the effect of fixer, conveniently will go up the lamellar body and fix with lower lamellar body centre gripping to convenient equipment is used.

Furthermore, both ends of the sample feeding groove and both ends of the sample discharging groove are semicircular, so that the flowing cells are prevented from being damaged due to collision.

Furthermore, all be the slope setting between circulation pipeline and the last lamellar body and the lateral wall of lamellar body down, do benefit to the length of extension circulation pipeline under the unchangeable condition of interval to hold more quantity of cell.

Compared with the prior art, the utility model discloses following beneficial effect has: through the arranged circulating pipeline and the connecting pipeline, after the cells are poured into the sample feeding groove along with the buffer solution, the cells flow in the circulating pipeline along with the buffer solution, and when the single circulating pipeline is blocked, the cells can enter the adjacent circulating pipeline through the connecting pipeline, so that the blocking condition is relieved, a larger number of cells can be accommodated, and the cell sorting efficiency is improved; through the fixer that sets up, the convenience is dismantled with lower lamellar body installation on going up the lamellar body, and it is comparatively convenient to use.

Drawings

Fig. 1 is a schematic diagram of the overall structure of a microfluidic chip for single cell sorting according to the present invention.

Fig. 2 is a cross-sectional view of the microfluidic chip for single cell sorting according to the present invention.

Fig. 3 is a schematic structural diagram of a holder for a microfluidic chip for single cell sorting according to the present invention.

In the figure: 1. an upper sheet body; 2. a lower sheet body; 3. a sample feeding groove; 4. a sample outlet groove; 5. a flow conduit; 6. connecting a pipeline; 7. a collection port; 8. a clamping plate; 9. a transverse plate; 10. a thread groove; 11. and (4) bolts.

Detailed Description

In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.

As shown in fig. 1-3, a micro-fluidic chip for unicellular separation, including last lamellar body 1 and the lower lamellar body 2 of laminating in last lamellar body 1 bottom, go up and be connected with the fixer between the periphery of lamellar body 1 and lower lamellar body 2, the top of going up lamellar body 1 is constructed and is advanced kind groove 3, the bottom of lamellar body 2 is constructed and is appeared a kind groove 4 down, go up and be constructed multichannel parallel arrangement's circulation pipeline 5 between the bottom of lamellar body 1 and the top of lower lamellar body 2, be connected with parallel arrangement's connecting tube 6 between circulation pipeline 5, circulation pipeline 5 is formed by the recess of last lamellar body 1 bottom and the recess laminating at lower lamellar body 2 top, after pouring the cell into advance kind groove 3 along with buffer solution, the cell flows in circulation pipeline 5 along with buffer solution, when producing the jam in single circulation pipeline 5, the cell can get into in adjacent circulation pipeline 5 through connecting tube 6 to alleviate the jam condition.

Wherein, the side wall of the lower sheet body 2 is provided with a plurality of collecting ports 7, the number of the collecting ports 7 corresponds to the number of the flowing pipelines 5, and the sampling cells are collected from the collecting ports 7.

Wherein, the fixer includes grip block 8, diaphragm 9, thread groove 10 and bolt 11, the lateral wall of grip block 8 laminating at last lamellar body 1 and lower lamellar body 2, and the both ends of grip block 8 all are connected with diaphragm 9 through integrated into one piece, the surface structure of diaphragm 9 has thread groove 10, and wears to be equipped with in the thread groove 10 with last lamellar body 1 and the bolt 11 of lower lamellar body 2 butt, under the effect of fixer, conveniently will go up lamellar body 1 and lower 2 centre grippings of lamellar body and fix to convenient equipment is used.

Wherein, the both ends of advancing the appearance groove 3 and the both ends of going out the appearance groove 4 all construct for semi-circular, avoid flowing cell to receive to collide with and produce the damage.

Wherein, all be the slope setting between circulation pipeline 5 and last lamellar body 1 and the lateral wall of lower lamellar body 2, do benefit to the length of extension circulation pipeline 5 under the unchangeable condition of interval to hold more quantity of cells.

It should be noted that, the utility model relates to a micro-fluidic chip for unicellular separation, pour the cell into and advance kind groove 3 in, and pour a small amount of buffer solution into, the cell flows along with buffer solution in taking a sample the groove, and get into in adjacent circulating line 5 in proper order, because the cell is the velocity of flow diverse in circulating line 5, there is the difference in the cell quantity in consequently circulating line 5, when the production of the great quantity is blockked up, unnecessary cell can get into in adjacent circulating line 5 through connecting tube 6, finally get into out in the kind groove 4, and discharge from collecting port 7 in proper order.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only illustrative of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the present invention, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a micro-fluidic chip for unicellular separation, includes last lamellar body (1) and the lower lamellar body (2) of laminating in last lamellar body (1) bottom, a serial communication port, go up and be connected with the fixer between the periphery of lamellar body (1) and lower lamellar body (2), the top of going up lamellar body (1) is constructed and is advanced kind groove (3), the bottom of lower lamellar body (2) is constructed and is appeared groove (4), it has multichannel parallel arrangement's circulation pipeline (5) to go up to construct between the bottom of lamellar body (1) and the top of lower lamellar body (2), be connected with parallel arrangement's connecting tube (6) between circulation pipeline (5), circulation pipeline (5) are formed by the recess of last lamellar body (1) bottom and the recess laminating at lower lamellar body (2) top.

2. The microfluidic chip for single-cell sorting according to claim 1, wherein: the side wall of the lower sheet body (2) is provided with a plurality of collecting openings (7), and the number of the collecting openings (7) corresponds to the number of the circulating pipelines (5).

3. The microfluidic chip for single-cell sorting according to claim 1, wherein: the fixer includes grip block (8), diaphragm (9), thread groove (10) and bolt (11), grip block (8) are laminated at the lateral wall of last lamellar body (1) and lower lamellar body (2), and the both ends of grip block (8) all are connected with diaphragm (9) through integrated into one piece, the surface structure of diaphragm (9) is equipped with thread groove (10), and wears to be equipped with in thread groove (10) and bolt (11) with last lamellar body (1) and lower lamellar body (2) butt.

4. The microfluidic chip for single-cell sorting according to claim 1, wherein: the two ends of the sample feeding groove (3) and the two ends of the sample discharging groove (4) are both constructed into a semicircle.

5. The microfluidic chip for single-cell sorting according to claim 1, wherein: the circulation pipeline (5) and the side walls of the upper sheet body (1) and the lower sheet body (2) are obliquely arranged.