CN217484169U

CN217484169U - Hyperspectral imaging microbial detection device

Info

Publication number: CN217484169U
Application number: CN202221223011.2U
Authority: CN
Inventors: 韦丽
Original assignee: Kunming Customs Technical Center
Current assignee: Kunming Customs Technical Center
Priority date: 2022-05-20
Filing date: 2022-05-20
Publication date: 2022-09-23
Anticipated expiration: 2032-05-20

Abstract

The application discloses device for hyperspectral imaging microbiological detection includes: the device comprises an image acquisition component and a processor; the image acquisition assembly is electrically connected with the processor; the method comprises the following steps: the device comprises a sample table, a plurality of illumination intensity sensors, a display, a guide rod, a light source hinge assembly, a screw rod driving assembly and a connecting plate; the image acquisition assembly is connected with the upper part of the guide rod in a sliding manner and arranged along the guide rod in a sliding manner; the sample table is arranged at the lower part of the guide rod; the guide rod is arranged outside one side edge of the sample table; a sample placing area is arranged on the top surface of the sample table; a plurality of illumination intensity sensors are arranged at intervals on the periphery of the sample placing area; the display is electrically connected with each illumination intensity sensor respectively. Through set up guide arm and screw drive subassembly respectively on light source board both sides, realize the reliable regulation to the height of the relative sample of light source to the realization is in high spectral imaging process, and freedom reliably adjusts the light source, thereby supplementary operating personnel high efficiency obtains the image that can be used to the chemometrics analysis.

Description

Hyperspectral imaging microbial detection device

Technical Field

The application relates to the technical field of microbial detection, in particular to a device for hyperspectral imaging microbial detection.

Background

In order to master the comprehensive growth condition of microorganisms in actual inspection and quarantine, the microorganisms need to be detected to obtain various indexes related to the microorganisms, so that the microorganism inspection and quarantine work is completed on samples. The original detection needs to count the number of microorganisms obtained after culturing and breeding the microorganisms in a sample under proper conditions. The detection efficiency is limited by the culture time of the microorganisms, and the detection result can be obtained only in 1-3 days. The hyperspectral imaging microbial detection technology utilizes the fact that when a light source irradiates the surface of an object to be detected, different substances to be detected have different absorbances, dispersivity and reflectivities to specific wavelengths due to different compositions and functional groups of different substances, and the total number of microbial colonies, spoilage bacteria and pathogenic bacteria on the surface of a sample are detected by acquiring spectral images of the sample to be detected and a standard substance.

As only the image needs to be obtained and the obtained image is analyzed through the chemometric tool, the result can be obtained, and the detection efficiency is greatly improved.

When the image is obtained, the brightness, the reflectivity and the like of the surface of the sample need to be adjusted to obtain the image meeting the analysis requirement. However, in the existing detection device, the difficulty of light source adjustment is high, and the manual adjustment of an operator is often needed, so that the image acquisition efficiency and the analysis efficiency are influenced.

SUMMERY OF THE UTILITY MODEL

The application provides a hyperspectral imaging device for microorganism detection, which is used for solving the technical problem that the projection angle of an imaging light source is difficult to adjust when the hyperspectral imaging in the prior art detects microorganisms.

The application provides a hyperspectral imaging device for microbial detection, include: the image acquisition assembly and the processor; the image acquisition assembly is electrically connected with the processor;

the method comprises the following steps: the device comprises a sample table, a plurality of illumination intensity sensors, a display, a guide rod, a light source hinge assembly, a screw driving assembly and a connecting plate; the image acquisition assembly is connected with the upper part of the guide rod in a sliding manner and arranged along the guide rod in a sliding manner;

the sample table is arranged at the lower part of the guide rod; the guide rod is arranged outside one side edge of the sample table; a sample placing area is arranged on the top surface of the sample table; a plurality of illumination intensity sensors are arranged at intervals on the periphery of the sample placing area; the illumination intensity sensor is arranged around the sample placing area; the display is electrically connected with each illumination intensity sensor respectively;

the connecting plate is arranged in the middle of the guide rod, and one side of the connecting plate is sleeved on the guide rod and arranged along the guide rod in a sliding manner; the connecting plate is in driving connection with the screw driving component; the screw driving component drives the connecting plate to reciprocate up and down along the guide rod; the screw driving component is arranged on the sample table; the screw driving component and the bottom surface of the guide rod are positioned on the same plane;

the light source hinge assembly is hinged on the bottom surface of the connecting plate and irradiates the sample placing area; a through hole is formed in the connecting plate, which is opposite to the sample placing area; the image acquisition end of the image acquisition assembly is arranged right opposite to the sample placement area; the light source hinge assemblies are symmetrically arranged on two sides of the through hole.

Preferably, the illumination intensity sensor includes: a first illumination intensity sensor, a second illumination intensity sensor, a third illumination intensity sensor, a fourth illumination intensity sensor; the first illumination intensity sensor, the second illumination intensity sensor, the third illumination intensity sensor and the fourth illumination intensity sensor are arranged on the periphery of the sample placing area.

Preferably, the first illumination intensity sensor is arranged on the sample table and arranged on a vertex angle at one side of the sample placing area; the second illumination intensity sensor is arranged on the sample table and is arranged on the other vertex angle of one side of the sample placing area; the third illumination intensity sensor is arranged on the sample table and arranged on a vertex angle on the other side of the sample placing area; the fourth illumination intensity sensor is arranged on the sample table and arranged on the other vertex angle of the other side of the sample placing area.

Preferably, the screw driving assembly includes: the device comprises a shell, a screw rod, a thread block, a sliding block and a driving motor; the thread blocks and the sliding blocks are symmetrically arranged on two side edges of the connecting plate; the thread block is in threaded connection with the screw rod; the sliding block is sleeved on the guide rod and arranged along the guide rod in a sliding manner; the shell is arranged on the outer side of the sample table; the screw rod is vertically arranged on the shell; one end of the screw rod is in driving connection with the driving motor.

Preferably, the method comprises the following steps: a PLC control module; the first illumination intensity sensor, the second illumination intensity sensor, the third illumination intensity sensor and the fourth illumination intensity sensor are respectively and electrically connected with the PLC control module; the PLC control module is electrically connected with the driving motor.

Preferably, the method comprises accommodating disposed within the housing: a drive gear and a driven gear; the driven gear is arranged on the bottom end of the screw and is kneaded with the driving gear; the driving gear is in driving connection with the driving motor.

Preferably, it comprises: adjusting the fixed seat and the extension rod; one end of the extension rod is provided with an adjusting fixing seat; the adjusting fixing seat is sleeved on the guide rod and is connected with the guide rod; the image acquisition assembly is arranged on the extension rod.

Preferably, the light source hinge assembly includes: the light source plate, the connecting rod, the first articulated shaft and the second articulated shaft are arranged on the light source plate; the connecting rod is vertically arranged on the bottom surface of the connecting plate and is connected with the connecting plate; the bottom surface of the connecting rod is hinged with one end of the movable rod through a first hinge shaft; the other end of the movable rod is hinged with the light source plate through a second hinged shaft.

Preferably, the light source hinge assembly includes: a cover body and a movable groove; the cover body is covered on the first articulated shaft; the top of the cover body is connected with a connecting rod; the movable groove is arranged on one side wall of the cover body; during adjustment, the movable rod is accommodated in the movable groove.

The beneficial effects that this application can produce include:

1) according to the device for detecting the microorganisms through hyperspectral imaging, the guide rods and the screw driving assemblies are respectively arranged on the two sides of the light source plate, so that the height of the light source relative to a sample can be reliably adjusted; simultaneously through articulating light source board and connecting plate, realize the effective regulation to light source and sample line contained angle to the realization is at high spectrum imaging in-process, and the freedom reliably adjusts the light source, thereby supplementary operating personnel high efficiency obtains the image that can be used to the chemometrics analysis.

2) The application provides a hyperspectral imaging is device for microbiological detection places regional periphery through the sample on examining test table top surface, encircles the circumference interval and sets up a plurality of illumination intensity sensors, and illumination intensity sensor is connected with the display electricity to acquire the illumination intensity data of different regions around the sample, the operating personnel of being convenient for effectively adjusts the light source angle as required, is favorable to shortening the image acquisition time.

Drawings

FIG. 1 is a schematic front view of a hyperspectral imaging microorganism detection device provided by the application;

FIG. 2 is a schematic perspective view of an apparatus for detecting microorganisms by hyperspectral imaging according to the present application;

fig. 3 is a schematic perspective view of a sample stage provided in the present application;

fig. 4 is a schematic perspective view of a light source plate provided in the present application;

FIG. 5 is a schematic view of a module connection structure provided herein;

illustration of the drawings:

111. a first illumination intensity sensor; 112. a second illumination intensity sensor; 113. a third illumination intensity sensor; 114. a fourth illumination intensity sensor; 115. a PLC control module; 116. a drive motor; 117. a display; 121. a sample stage; 122. a base; 131. a housing; 133. a screw; 134. a thread block; 143. a slider; 141. a connecting plate; 142. a guide bar; 149. a through hole; 135. a driving gear; 136. a driven gear; 145. adjusting the fixed seat; 148. an extension rod; 146. an image acquisition component; 147. a processor; 15. a light source hinge assembly; 151. a light source board; 152. a connecting rod; 153. a cover body; 154. a first hinge shaft; 155. a second hinge shaft; 156. a movable groove.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Technical features which are not used for solving the technical problems of the present application are all arranged or installed according to a common method in the prior art, and are not described herein in a repeated manner.

Referring to fig. 1-5, the application provides a device for hyperspectral imaging microorganism detection, includes: an image acquisition component 146, a processor 147; the image acquisition assembly 146 is electrically connected with the processor 147;

the method comprises the following steps: the device comprises a sample table 121, a plurality of illumination intensity sensors, a display 117, a guide rod 142, a light source hinge assembly 15, a screw 133 driving assembly and a connecting plate 141; the image acquisition assembly 146 is connected with the upper part of the guide rod 142 in a sliding manner and arranged along the guide rod 142 in a sliding manner;

the sample stage 121 is arranged at the lower part of the guide rod 142; the guide rod 142 is arranged outside one side of the sample stage 121; a sample placing area is arranged on the top surface of the sample stage 121; a plurality of illumination intensity sensors are arranged at intervals on the periphery of the sample placing area; the illumination intensity sensor is arranged around the sample placing area; the display 117 is electrically connected to each of the light intensity sensors;

the connecting plate 141 is arranged in the middle of the guide rod 142, and one side of the connecting plate 141 is sleeved on the guide rod 142 and arranged along the guide rod 142 in a sliding manner; the connecting plate 141 is in driving connection with the screw 133 driving component; the screw 133 drives the assembly to drive the connecting plate 141 to reciprocate up and down along the guide rod 142; the screw 133 driving component is arranged on the sample table 121; the screw 133 driving component and the bottom surface of the guide rod 142 are in the same plane;

the light source hinge assembly 15 is hinged on the bottom surface of the connecting plate 141 and arranged to irradiate the sample placing area; a through hole 149 is formed in the connecting plate 141 opposite to the sample placing area; the image acquisition end of the image acquisition assembly 146 is arranged right opposite to the sample placement area; the light source hinge assemblies 15 are symmetrically disposed at both sides of the through hole 149.

According to the arrangement, an operator can control the longitudinal height of the light source hinge assembly 15 relative to the sample table 121 through the guide rod 142 and the screw 133 driving assembly, and simultaneously control the irradiation angle of the light source towards the sample through the hinge shaft, so that the luminosity irradiation intensity can be adjusted according to the image acquisition requirement. The operator can obtain the light intensity of different positions through a plurality of sensors arranged on the top surface of the sample table 121, and adjust according to the numerical value, so that the reliability of the illumination intensity adjustment is improved.

When in use, the image acquisition assembly 146 and the processor 147 are communicated firstly, and the height of the image acquisition assembly 146 relative to the sample is adjusted after the sample is placed; after the light source is started, the screw 133 drives the component to adjust and control the interval between the light source and the sample on the connecting plate 141 according to the numerical value on the display 117, and after the light intensity of the sample placing area is adjusted, image acquisition is carried out, and chemometric analysis is carried out to obtain various parameters of the microorganisms on the surface of the sample. The size of the through hole 149 provided in the present application can be adjusted according to the size of the sample so as to acquire the sample image without shielding.

The image capturing component 146 used in the present application is a component commonly used for hyperspectral imaging, for example, including: the CCD camera, the spectrometer and the lens, and the specific connection and arrangement mode are not described in detail here. The processor 147 may be a computer or other like device configured to perform hyperspectral analytically stoichiometric processing on the acquired image of the sample.

Preferably, the illumination intensity sensor includes: a first illumination intensity sensor 111, a second illumination intensity sensor 112, a third illumination intensity sensor 113, a fourth illumination intensity sensor 114; the first illumination intensity sensor 111, the second illumination intensity sensor 112, the third illumination intensity sensor 113 and the fourth illumination intensity sensor 114 are arranged at the periphery of the sample placement area.

Preferably, the first light intensity sensor 111 is arranged on the sample stage 121 and arranged on a top corner of one side of the sample placement area; the second illumination intensity sensor 112 is arranged on the sample table 121 and arranged on the other vertex angle on one side of the sample placing area; the third illumination intensity sensor 113 is arranged on the sample table 121 and arranged on a vertex angle on the other side of the sample placing area; the fourth illumination intensity sensor 114 is disposed on the sample stage 121 and is disposed on the other vertex angle of the other side of the sample placement area.

The number is minimum according to the setting, the accuracy of obtaining the illumination intensity is high, and the adjusting effect is good.

Preferably, the screw 133 driving assembly comprises: the shell 131, the screw 133, the thread block 134, the sliding block 143 and the driving motor 116; the thread block 134 and the slide block 143 are symmetrically arranged on two side edges of the connecting plate 141; the screw block 134 is in threaded connection with the screw 133; the sliding block 143 is sleeved on the guide rod 142 and is arranged along the guide rod 142 in a sliding manner; the shell 131 is arranged outside the sample stage 121; the screw 133 is vertically arranged on the housing 131; one end of the screw 133 is drivingly connected to the driving motor 116.

Preferably, the method comprises the following steps: a PLC control module 115; the first illumination intensity sensor 111, the second illumination intensity sensor 112, the third illumination intensity sensor 113 and the fourth illumination intensity sensor 114 are electrically connected with the PLC control module 115 respectively; the PLC control module 115 is electrically connected to the driving motor 116. The light source plate 151 can be automatically adjusted to the height of the sample by presetting the light intensity value. The adjustment of the illumination intensity to be used is set by a method commonly used in the art, and not described herein, and the orientation angle of the light source plate 151 can be preset when this automatic adjustment is used.

Preferably, it includes housing disposed within the housing 131: a drive gear 135 and a driven gear 136; the driven gear 136 is disposed on the bottom end of the screw 133 and kneaded with the driving gear 135; the driving gear 135 is in driving connection with the driving motor 116. This arrangement enables the raising and lowering of the link plate 141.

In a specific embodiment, in order to obtain a stable lifting force of the connecting plate 141, the screw 133 driving assemblies may be arranged in pairs, a group of screw 133 driving assemblies is further arranged outside the guide rods 142, and after the screw 133 driving assemblies are in driving connection with the connecting plate 141, the driving motor 116 and the screw 133 driving assembly on the other side of the connecting plate 141 rotate synchronously, so as to improve the up-and-down movement stability of the connecting plate 141.

In one embodiment, the method comprises the following steps: a base 122; the shell 131, the sample table 121 and the guide rod 142 are arranged on the top surface of the base 122, so that the whole device can be moved conveniently, and a flat plane can be obtained; the display 117 may also be disposed on an outer sidewall of the base 122.

Preferably, the method comprises the following steps: the adjusting fixing seat 145 and the extension rod 148; one end of the extension rod 148 is provided with an adjusting fixed seat 145; the adjusting fixing seat 145 is sleeved on the guide rod and connected with the guide rod; the image capturing assembly 146 is disposed on the extension rod 148. Set up according to this and be convenient for adjust, fix the position of extension rod 148 on the guide bar through adjusting fixing base 145, used regulation fixing base 145 can wear to locate on the barrel lateral wall for through the bolt to realize fixedly with the guide bar lateral wall butt, loosen the structure that realizes position control.

Preferably, the light source hinge assembly 15 includes: a light source plate 151, a connection rod 152, a first hinge shaft 154, and a second hinge shaft 155; the connecting rod 152 is vertically arranged on the bottom surface of the connecting plate 141 and connected with the connecting plate 141; the bottom surface of the connecting rod 152 is hinged to one end of the movable rod by a first hinge shaft 154; the other end of the movable rod is hinged to the light source plate 151 through a second hinge shaft 155. According to the arrangement, multi-angle adjustment of the light source plate 151 is facilitated, and the adjustment flexibility of the light source irradiation intensity is improved. Light source board 151 used in this application is a board with a plurality of LED light bars arranged on the surface.

Preferably, the light source hinge assembly 15 includes: a cover body 153, a movable groove 156; the cover body 153 covers the first hinge shaft 154; the top of the cover 153 is connected with the connecting rod 152; the movable groove 156 is opened on one side wall of the cover 153; upon adjustment, the movable lever is received into the movable slot 156.

The angle adjustment range of the light source plate 151 is expanded by providing the movable groove 156, increasing the movable space.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A device for hyperspectral imaging microbiological detection comprising: an image acquisition assembly (146), a processor (147); the image acquisition assembly (146) is electrically connected with the processor (147); it is characterized in that the preparation method is characterized in that,

the method comprises the following steps: the device comprises a sample table (121), a plurality of illumination intensity sensors, a display (117), a guide rod (142), a light source hinge assembly (15), a screw rod (133) driving assembly and a connecting plate (141); the image acquisition assembly (146) is connected with the upper part of the guide rod (142) in a sliding manner and arranged along the guide rod (142) in a sliding manner;

the sample table (121) is arranged at the lower part of the guide rod (142); the guide rod (142) is arranged outside one side of the sample table (121); a sample placing area is arranged on the top surface of the sample table (121); a plurality of light intensity sensors are arranged at intervals on the periphery of the sample placement area; the illumination intensity sensor is arranged around the sample placing area; the display (117) is electrically connected with each illumination intensity sensor;

the connecting plate (141) is arranged in the middle of the guide rod (142), and one side of the connecting plate (141) is sleeved on the guide rod (142) and arranged along the guide rod (142) in a sliding manner; the connecting plate (141) is in driving connection with the screw rod (133) driving component; the screw (133) drives the connecting plate (141) to reciprocate up and down along the guide rod (142) by the driving component; the screw (133) driving component is arranged on the sample table (121); the screw rod (133) driving assembly and the bottom surface of the guide rod (142) are positioned on the same plane;

the light source hinge assembly (15) is hinged on the bottom surface of the connecting plate (141) and is used for irradiating the sample placing area; a through hole (149) is arranged on the connecting plate (141) and right opposite to the sample placing area; the image acquisition end of the image acquisition assembly (146) is arranged right opposite to the sample placement area; the light source hinge assemblies (15) are symmetrically arranged on two sides of the through hole (149).

2. The hyperspectral imaging apparatus for microorganism detection as claimed in claim 1, wherein the illumination intensity sensor comprises: a first illumination intensity sensor (111), a second illumination intensity sensor (112), a third illumination intensity sensor (113), and a fourth illumination intensity sensor (114); the first illumination intensity sensor (111), the second illumination intensity sensor (112), the third illumination intensity sensor (113) and the fourth illumination intensity sensor (114) are arranged at the periphery of the sample placement area.

3. The hyperspectral imaging microorganism detection apparatus according to claim 2, wherein the first illumination intensity sensor (111) is arranged on the sample stage (121) and arranged on a vertex angle on one side of the sample placement area; the second illumination intensity sensor (112) is arranged on the sample table (121) and is arranged on the other vertex angle of one side of the sample placing area; the third illumination intensity sensor (113) is arranged on the sample table (121) and is arranged on a vertex angle on the other side of the sample placing area; and a fourth illumination intensity sensor (114) is arranged on the sample table (121) and arranged on the other top corner of the other side of the sample placing area.

4. The apparatus for hyperspectral imaging microbiological detection according to claim 2 wherein the screw (133) drive assembly comprises: the device comprises a shell (131), a screw rod (133), a thread block (134), a sliding block (143) and a driving motor (116); the thread block (134) and the slide block (143) are symmetrically arranged on two side edges of the connecting plate (141); the thread block (134) is in threaded connection with the screw (133); the sliding block (143) is sleeved on the guide rod (142) and arranged along the guide rod (142) in a sliding manner; the shell (131) is arranged on the outer side of the sample table (121); the screw (133) is vertically arranged on the shell (131); one end of the screw rod (133) is in driving connection with the driving motor (116).

5. The hyperspectral imaging apparatus for microorganism detection according to claim 4, comprising: a PLC control module (115); the first illumination intensity sensor (111), the second illumination intensity sensor (112), the third illumination intensity sensor (113) and the fourth illumination intensity sensor (114) are respectively electrically connected with the PLC control module (115); the PLC control module (115) is electrically connected with the driving motor (116).

6. The hyperspectral imaging microorganism detection apparatus according to claim 4, comprising: a drive gear (135) and a driven gear (136); the driven gear (136) is arranged on the bottom end of the screw (133) and is kneaded with the driving gear (135); the driving gear (135) is in driving connection with the driving motor (116).

7. The hyperspectral imaging apparatus for microorganism detection according to claim 1, comprising: an adjusting fixed seat (145) and an extension rod (148); one end of the extension rod (148) is provided with an adjusting fixed seat (145); the adjusting fixing seat (145) is sleeved on the guide rod and connected with the guide rod; the image acquisition assembly (146) is arranged on the extension rod (148).

8. The apparatus for hyperspectral imaging microbiological detection according to claim 1 wherein the light source hinge assembly (15) comprises: a light source plate (151), a connecting rod (152), a first hinge shaft (154), and a second hinge shaft (155); the connecting rod (152) is vertically arranged on the bottom surface of the connecting plate (141) and is connected with the connecting plate (141); the bottom surface of the connecting rod (152) is hinged with one end of the movable rod through a first hinge shaft (154); the other end of the movable rod is hinged with the light source plate (151) through a second hinge shaft (155).

9. The apparatus for hyperspectral imaging microbiological detection according to claim 8 wherein the light source hinge assembly (15) comprises: a cover body (153) and a movable groove (156); the cover body (153) covers the first articulated shaft (154); the top of the cover body (153) is connected with the connecting rod (152); the movable groove (156) is arranged on one side wall of the cover body (153); during adjustment, the movable rod is received into the movable slot (156).