CN210037615U - Chlorophyll fluorescence imaging device - Google Patents
Chlorophyll fluorescence imaging device Download PDFInfo
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- CN210037615U CN210037615U CN201920803947.4U CN201920803947U CN210037615U CN 210037615 U CN210037615 U CN 210037615U CN 201920803947 U CN201920803947 U CN 201920803947U CN 210037615 U CN210037615 U CN 210037615U
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Abstract
The utility model relates to a chlorophyll fluorescence imaging technology field provides a chlorophyll fluorescence imaging device, include: the device comprises a closed optical darkroom cabinet, a first directional component, a second directional component and a third directional component, wherein the first directional component, the second directional component and the third directional component are used for adjusting the position of a detector; a data processing device for sample analysis, said data processing device being connected to said detector, said data processing device being connected to said LED light source; the control box is positioned on the outer side of the optical darkroom cabinet, is respectively connected with the first, second and third directional components, and controls the first, second and third directional components to operate. By the structure, the problem of online detection of a sample to be detected is solved, the detector capable of automatically adjusting the position is arranged, the detector can be automatically adjusted to a proper measuring position according to the position and the height of the sample to be detected, and the data processing device connected with the detector can realize automatic batch measurement according to a preset program.
Description
Technical Field
The utility model belongs to the technical field of chlorophyll fluorescence imaging technique and specifically relates to indicate and provide a chlorophyll fluorescence imaging device.
Background
The chlorophyll fluorescence phenomenon refers to a process that after dark-adapted plants are subjected to illumination conditions, chlorophyll fluorescence rapidly rises to a maximum value, then gradually falls, and finally reaches a stable value. Most changes of crop photosynthesis can be reflected by chlorophyll fluorescence, and the chlorophyll fluorescence is used as a probe for photosynthesis research, has the characteristics of specificity and high sensitivity, can quickly reflect the physiological and ecological conditions of plants, can realize nondestructive detection, and has wide application in a plurality of fields such as mutant strain screening, pest and disease detection, phenotype analysis and the like. However, the problems that automatic batch detection, online detection and the like cannot be realized exist at present, so that the detection of a sample to be detected is slow, and the efficiency is low. Therefore, a chlorophyll fluorescence imaging device for automatic batch online detection is urgently needed.
Disclosure of Invention
In order to solve the technical problem, an object of the present invention is to provide a chlorophyll fluorescence imaging device, which not only solves the problem of online detection of samples to be detected, but also provides a detector capable of automatically adjusting the position of the sample to be detected, and can automatically adjust the position of the sample to be detected and the height of the sample to be detected to a suitable measurement position, and a data processing device connected to the detector can realize automatic batch measurement according to a preset program.
In order to achieve the above purpose, the utility model adopts the following technical scheme: provided is a chlorophyll fluorescence imaging apparatus including:
the device comprises a closed optical darkroom cabinet, wherein a sample table is arranged at the bottom end inside the optical darkroom cabinet, and an organic cabinet door is arranged on one side of the optical darkroom cabinet;
a first direction member provided in the optical darkroom cabinet, the first direction member including a first connection plate and a first adjustment mechanism, the first adjustment mechanism being fixed to a lower side of a top plate of the optical darkroom cabinet, the first connection plate being reciprocally movable in an axial direction of the first adjustment mechanism;
the second direction component is arranged on the first connecting plate and comprises a second connecting plate and a second adjusting mechanism, the second adjusting mechanism is fixed on the first connecting plate, and the second connecting plate reciprocates along the axis direction of the second adjusting mechanism;
the third direction component is arranged on the second connecting plate, the upper end of the third direction component is fixedly connected with the second connecting plate, the lower end of the third direction component is connected with a lamp panel, the lamp panel reciprocates along the axis direction of the third direction component, an LED light source and a detector are arranged on the lamp panel, and the detector is used for imaging a sample to be detected;
a data processing device for sample analysis, the data processing device being outside the optical darkroom cabinet, the data processing device being connected to the detector, the data processing device being connected to the LED light source;
the control box is arranged on the outer side of the optical darkroom cabinet, is respectively connected with the first, second and third directional components, and controls the first, second and third directional components to operate.
In this embodiment, preferably, the first adjusting mechanism includes a first motor, a first guide rail and a first slider, the first motor is fixed to a side plate of the optical darkroom cabinet, the first guide rail is fixed to a lower side of a top plate of the optical darkroom cabinet, an output shaft of the first motor drives the first slider to reciprocate on the first track, and the first slider is fixedly connected to the first connecting plate.
In this embodiment, preferably, the second adjusting mechanism includes a second motor, a second guide rail and a second slider, the second motor is fixed to one side of the first connecting plate, the second guide rail is fixed to the first connecting plate, an output shaft of the second motor drives the second slider to reciprocate on the second track, and the second slider is fixedly connected to the second connecting plate.
In this embodiment, the third directional component is preferably an electric push rod.
In this embodiment, preferably, the LED light source includes a plurality of first diodes, and the first diodes are blue diodes.
In this embodiment, preferably, the LED light source further includes a plurality of second diodes, and the second diodes are red diodes.
In this embodiment, preferably, the LED light source further includes a plurality of third diodes, the third diodes are near-infrared diodes, and the second diodes and the third diodes are arranged in pairs.
In this embodiment, preferably, the sample stage is provided with a plurality of cavity trays, and the cavity trays are used for placing samples to be measured.
In this embodiment, it is preferable that the cavity plate has a plurality of cavities for placing the samples to be tested.
The utility model provides a chlorophyll fluorescence image device can bring following at least one beneficial effect:
1. the utility model discloses in, through chlorophyll fluorescence imaging device, not only solved the on-line measuring problem of the sample that awaits measuring to set up the detector of automatic adjustment position, can be according to the position of the sample that awaits measuring and high automatically regulated to suitable measuring position, the data processing apparatus who links to each other with the detector can realize automatic batch measurement according to predetermined procedure.
2. The utility model discloses in, chlorophyll fluorescence image device has degree of automation height, detects characteristics such as quick, harmless and application scope is extensive, is applicable to but not limited to the detection of samples such as plant, branch and leaf, seed and alga.
Drawings
Fig. 1 is a schematic view of the internal structure of the chlorophyll fluorescence imaging apparatus according to the first embodiment.
Fig. 2 is a schematic structural diagram of the chlorophyll fluorescence imaging apparatus in the first embodiment.
Fig. 3 is a schematic structural diagram of an adjusting mechanism in the first embodiment.
Fig. 4 is a schematic structural diagram of a fourth plug of the present embodiment.
The reference numbers illustrate:
1. the system comprises an optical darkroom cabinet, 11 a cabinet door, 2 a control box, 3 a data processing device, 4 a first direction component, 5 a second direction component, 6 a third direction component, 7 a lamp panel, 71.LED light sources, 72 a detector, 8 a sample table, 81 a hole tray and 811 a hole groove.
Detailed Description
While the present invention may be susceptible to embodiment in different forms, there is shown in the drawings and will herein be described in detail, specific embodiments thereof with the understanding that the present description is to be considered as an exemplification of the principles of the invention and is not intended to limit the invention to that as illustrated herein.
Thus, a feature indicated in this specification will serve to explain one of the features of an embodiment of the invention, and not to imply that every embodiment of the invention must have the described feature. Further, it should be noted that this specification describes many features. Although some features may be combined to show a possible system design, these features may also be used in other combinations not explicitly described. Thus, the combinations illustrated are not intended to be limiting unless otherwise specified.
In the embodiments shown in the drawings, the directions (such as up, down, left, right, front, and rear) are used to explain the structure and movement of the various components of the present invention not absolutely, but relatively. These illustrations are appropriate when these components are in the positions shown in the figures. If the description of the positions of these components changes, the indication of these directions changes accordingly.
The preferred embodiments of the present invention will be further described in detail with reference to the accompanying drawings.
In an embodiment, as shown in fig. 1 to 3, the present embodiment provides a chlorophyll fluorescence imaging apparatus including: an optical darkroom cabinet 1, an adjusting mechanism, a control box 2 and a data processing device 3. The inside of optics darkroom rack 1 is a inclosed light space that separates, and the front side of optics darkroom rack 1 is equipped with cabinet door 11, and cabinet door 11 is equipped with bilateral symmetry's two, and the left side of left cabinet door is connected with optics darkroom rack 1 through the hinge rotation, and the right side of right cabinet door is connected with optics darkroom rack 1 through the hinge rotation. The inside bottom of optics darkroom rack 1 is equipped with sample platform 8, and sample platform 8 is used for placing the sample that awaits measuring, and wherein, the sample that awaits measuring can be plantlet, plant branch and leaf, seed or alga etc..
The adjusting mechanism is installed inside the optical darkroom cabinet 1, the adjusting mechanism is connected with the control box 2, and the control box 2 controls the adjusting mechanism to automatically adjust the position and the height of the detector 72. The control box 2 is a PLC control box, the control box 2 is fixed on the left side of the optical darkroom cabinet 1, and the control box 2 is connected with an external power supply. The adjustment mechanism includes a first direction member 4, a second direction member 5, and a third direction member 6. Wherein, first direction component 4 includes first connecting plate, first motor, first guide rail and first slider, and first guide rail is fixed in the downside of 1 roof of optics darkroom rack, and first motor is fixed in the upper end of 1 left side board of optics darkroom rack, and first slider adaptation is installed on first guide rail, the output shaft and the first slider fixed connection of first motor, and first motor can drive first slider reciprocating motion on first track under the control of PLC control box. The axis direction of the first track is the X-axis direction, and the first connecting plate is fixed at the lower end of the first sliding block through screws. The second direction component 5 comprises a second connecting plate, a second motor, a second guide rail and a second sliding block, the second guide rail is fixed on the lower side of the first connecting plate, the second motor is fixed on the lower side of the first connecting plate, the second sliding block is installed on the second guide rail in an adaptive mode, an output shaft of the second motor is fixedly connected with the second sliding block, and the second motor can drive the second sliding block to move on a second track in a reciprocating mode under the control of the PLC control box. The axis direction of the second track is the Y-axis direction, and the second connecting plate is fixed at the lower end of the second sliding block through screws. The upper end and the second connecting plate fixed connection of third direction component 6, the lower extreme and the lamp plate 7 of third direction component 6 are connected. The third direction component 6 is an electric push rod, the electric push rod can drive the lamp panel 7 to reciprocate in the axis direction of the electric push rod under the control of the PLC control box, and the axis direction of the electric push rod is the Z-axis direction.
The lamp panel 7 is provided with an LED light source 71 and a detector 72, the LED light source 71 is used for providing preset light, the detector 72 is used for imaging a sample to be detected, and the detector 72 is a CCD detector. The data processing device 3 is connected with the detector 72, and the data processing device 3 is connected with the LED light source 71; the data processing device 3 is used for analyzing and processing the imaging result of the sample to be detected, meanwhile, by installing preset software on the data processing device, various actions of the LED light source 71 and the detector 72 can be controlled, and the data processing device 3 can be a notebook computer.
In the embodiment, the preset software is installed on the notebook computer, so that the sample to be detected can be monitored in real time, and meanwhile, the sample to be detected can be detected on line; the PLC electric cabinet controls the operation of the adjusting mechanism, so that the position of the detector is automatically adjusted, the detector can be automatically adjusted to a proper measuring position according to the position and the height of a sample to be measured, and the measuring precision is improved; samples to be detected are placed on the sample table 8 in batches, the position of the detector is automatically adjusted through the PLC, the data processing device 3 automatically controls various actions of the LED light source 71 and the detector 72, and automatic batch processing of the samples to be detected of the high-flux modulated chlorophyll fluorescence imaging device is achieved.
In the second embodiment, as shown in fig. 1 to 3, in addition to the first embodiment, the adjusting mechanism is installed inside the optical darkroom cabinet 1, the adjusting mechanism is connected to the control box 2, and the control box 2 controls the adjusting mechanism to automatically adjust the position and height of the detector 72. The control box 2 is a PLC control box, the control box 2 is fixed on the left side of the optical darkroom cabinet 1, and the control box 2 is connected with an external power supply. The adjustment mechanism includes a first direction member 4, a second direction member 5, and a third direction member 6. Wherein, first direction component 4 includes first connecting plate, a first motor, first lead screw and first lead screw nut, first lead screw is fixed at the downside of 1 roof of optics darkroom rack, first motor is fixed in the upper end of 1 left side board of optics darkroom rack, first lead screw nut adaptation cover is on first lead screw, the output shaft of first motor and the one end fixed connection of first lead screw, first motor is under the control of PLC control box, the output shaft of first motor drives first lead screw and rotates round its own axis for first lead screw nut is reciprocating motion along the axis direction of first lead screw. The axis direction of the first lead screw is the X-axis direction, and the first connecting plate is fixed at the lower end of the first lead screw nut through a screw. Second direction component 5 includes the second connecting plate, the second motor, second lead screw and second lead screw nut, the second lead screw is fixed at the downside of first connecting plate, the downside at first connecting plate is fixed to the second motor, second lead screw nut adaptation cover is on the second lead screw, the output shaft of second motor and the one end fixed connection of second lead screw, the second motor is under the control of PLC control box, the output shaft of second motor drives the second lead screw and rotates round its own axis, make second lead screw nut be reciprocating motion along the axis direction of second lead screw. The axis direction of the second track is the Y-axis direction, and the second connecting plate is fixed at the lower end of the second sliding block through screws. The upper end and the second connecting plate fixed connection of third direction component 6, the lower extreme and the lamp plate 7 of third direction component 6 are connected. The third direction component 6 is an electric push rod, the electric push rod can drive the lamp panel 7 to reciprocate in the axis direction of the electric push rod under the control of the PLC control box, and the axis direction of the electric push rod is the Z-axis direction.
In the third embodiment, as shown in fig. 1 to 3, based on the first and second embodiments, the LED light source 7 includes a plurality of first diodes, and the first diodes are light-emitting blue diodes with parallel optical correction. The LED light source 7 further comprises a plurality of second diodes, which are red diodes. The LED light source 7 further includes a plurality of third diodes, the third diodes being near-infrared diodes, the second diodes and the third diodes being arranged in pairs. Preferably, the LED light source comprises 44 super-luminescent blue diodes with parallel optical correction, the wavelength of the blue light is 450nm, and a homogeneous light field can be generated at a distance of 17-20cm from the light source. The light-emitting blue diode not only provides modulated measurement light, but also actinic light and saturation pulses. Preferably, the LED light source further comprises 32 diodes for measuring PAR absorption coefficient. Wherein, 16 red diodes and 16 near infrared diodes, the wavelength of the red light is 660nm, and the wavelength of the near infrared is 780 nm. The red diodes are arranged in pairs with the near-infrared diodes, each pair comprising one red diode and one near-infrared diode. The lens of the diode can be removed in order to obtain a homogenous light field on the sample. The preset software on the notebook computer controls the light emission fluorescence excitation light source with specific wavelength, the CCD detector detects fluorescence of the plant after being excited and performs fluorescence imaging, and the CCD detector collects fluorescence information and transmits the data to the notebook computer through a network cable.
In the fourth embodiment, as shown in fig. 4, in addition to the first, second and third embodiments, a plurality of hole trays 81 are provided on the sample stage 8, a plurality of hole grooves 811 are provided on the hole trays 81, and the hole trays 81 and the hole grooves 811 are used for placing a sample to be measured. In order to facilitate the placement of different samples to be tested, the cavity plate 81 and the cavity 811 may be configured to have different sizes and different shapes. For example: for detecting the plantlets, the hole tray 81 can be designed into a disc shape, and a vessel for loading the plantlets is directly placed in the disc-shaped hole tray; the hole tray 81 can be set to be rectangular for detecting seeds, the hole tray 81 is uniformly provided with a plurality of hole grooves 811, and various seeds can be respectively placed in the hole grooves 811 for batch detection. The LED light source 71 and the detector 72 on the lamp panel 7 are installed at the lower end of the adjusting device, and can be controlled by the PLC control box in three directions of the X, Y, Z shaft, and accurately positioned on the plug tray 81 or each plug groove 811 in the plug tray 81.
It should be noted that the above embodiments can be freely combined as necessary. The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A chlorophyll fluorescence imaging apparatus, comprising:
the device comprises a closed optical darkroom cabinet, wherein a sample table is arranged at the bottom end inside the optical darkroom cabinet, and an organic cabinet door is arranged on one side of the optical darkroom cabinet;
a first direction member provided in the optical darkroom cabinet, the first direction member including a first connection plate and a first adjustment mechanism, the first adjustment mechanism being fixed to a lower side of a top plate of the optical darkroom cabinet, the first connection plate being reciprocally movable in an axial direction of the first adjustment mechanism;
the second direction component is arranged on the first connecting plate and comprises a second connecting plate and a second adjusting mechanism, the second adjusting mechanism is fixed on the first connecting plate, and the second connecting plate reciprocates along the axis direction of the second adjusting mechanism;
the third direction component is arranged on the second connecting plate, the upper end of the third direction component is fixedly connected with the second connecting plate, the lower end of the third direction component is connected with a lamp panel, the lamp panel reciprocates along the axis direction of the third direction component, an LED light source and a detector are arranged on the lamp panel, and the detector is used for imaging a sample to be detected;
a data processing device for sample analysis, the data processing device being outside the optical darkroom cabinet, the data processing device being connected to the detector, the data processing device being connected to the LED light source;
the control box is arranged on the outer side of the optical darkroom cabinet, is respectively connected with the first, second and third directional components, and controls the first, second and third directional components to operate.
2. The chlorophyll fluorescence imaging apparatus of claim 1, wherein:
the first adjusting mechanism comprises a first motor, a first guide rail and a first sliding block, the first motor is fixed on a side plate of the optical darkroom cabinet, the first guide rail is fixed on the lower side of a top plate of the optical darkroom cabinet, an output shaft of the first motor drives the first sliding block to move on the first guide rail in a reciprocating mode, and the first sliding block is fixedly connected with the first connecting plate.
3. The chlorophyll fluorescence imaging apparatus of claim 2, wherein:
the second adjusting mechanism comprises a second motor, a second guide rail and a second sliding block, the second motor is fixed on one side of the first connecting plate, the second guide rail is fixed on the first connecting plate, an output shaft of the second motor drives the second sliding block to reciprocate on the second guide rail, and the second sliding block is fixedly connected with the second connecting plate.
4. A chlorophyll fluorescence imaging apparatus according to claim 3, wherein:
the third direction component is an electric push rod.
5. The chlorophyll fluorescence imaging apparatus of claim 1, wherein:
the LED light source comprises a plurality of first diodes, and the first diodes are blue diodes.
6. The chlorophyll fluorescence imaging apparatus of claim 5, wherein:
the LED light source further comprises a plurality of second diodes, and the second diodes are red diodes.
7. The chlorophyll fluorescence imaging apparatus of claim 6, wherein:
the LED light source further comprises a plurality of third diodes, the third diodes are near-infrared diodes, and the second diodes and the third diodes are arranged in pairs.
8. The chlorophyll fluorescence imaging apparatus of claim 1, wherein:
the sample platform is provided with a plurality of hole trays, and the hole trays are used for placing samples to be tested.
9. The chlorophyll fluorescence imaging apparatus of claim 8, wherein:
and the hole tray is provided with a plurality of hole grooves, and the hole grooves are used for placing samples to be tested.
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| CN201920803947.4U CN210037615U (en) | 2019-05-30 | 2019-05-30 | Chlorophyll fluorescence imaging device |
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| CN201920803947.4U CN210037615U (en) | 2019-05-30 | 2019-05-30 | Chlorophyll fluorescence imaging device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111982818A (en) * | 2020-07-30 | 2020-11-24 | 山东省分析测试中心 | Device for detecting liquid crystal droplet optical digital signal and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111982818A (en) * | 2020-07-30 | 2020-11-24 | 山东省分析测试中心 | Device for detecting liquid crystal droplet optical digital signal and application thereof |
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