CN217466593U

CN217466593U - Blood sample analyzer and turbidimetric apparatus

Info

Publication number: CN217466593U
Application number: CN202220240590.5U
Authority: CN
Inventors: 张浩然; 汪东生
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2022-01-28
Filing date: 2022-01-28
Publication date: 2022-09-20
Anticipated expiration: 2032-01-28

Abstract

The utility model is suitable for a sample analysis equipment field discloses a blood sample analysis appearance and than turbid measuring device. The blood sample analyzer comprises a reagent supply device, a turbidimetric measurement device and a control device; the turbidimetric measurement device comprises a reaction cell, a front light assembly and a rear light assembly, wherein the reaction cell is provided with an inner cavity, and the rear light assembly comprises a scattered light channel and a scattered light collecting component; the inner side wall of the inner cavity comprises an incident surface and an emergent surface; when the front light assembly emits parallel light rays towards the reaction cell, a transmitted light beam is formed on the reaction cell, the transmitted light beam is provided with a first edge light beam with the minimum distance to the scattered light collecting component, and the incident surface is provided with a first edge line which is intersected with the first edge light beam; when a point on the first edge line is taken as a first reference point for light emission, the scattered light collection member can collect each light emitted from the first reference point satisfying the following condition: the first initial emission line segment makes an angle with the first edge beam of greater than or equal to 12.3 °.

Description

Blood sample analyzer and turbidimetric apparatus

Technical Field

The utility model relates to a sample analysis equipment field especially relates to a than turbid measuring device and have this than turbid measuring device's blood sample analyzer.

Background

At present, two methods commonly used for detecting specific protein parameters in a sample are transmission turbidimetry and scattering turbidimetry. The sample analyzer provided by the traditional technology adopts a nephelometry to detect specific protein parameters of a sample, and the working principle is as follows: the amount of a particular protein in a sample can be characterized by collecting scattered light over a range of angles produced by the sample and analyzing the change in intensity of the collected scattered light. Specifically, the sample analyzer in the conventional art includes a turbidimetric measurement device including a reaction cell, a front light module for emitting light toward the reaction cell, and a rear light module for collecting scattered light generated by the front light module irradiating a sample. However, the sample analyzer in the conventional art does not clearly define the boundary condition according to which the angle range of the scattered light collected by the backlight assembly is determined, so that the design difficulty of the backlight assembly is high, and the light collected by the backlight assembly is not reasonable. On one hand, the accuracy of the detection result of the specific protein is low, and especially when the content of the specific protein in the sample is low, the accuracy of the detection result is low; on the other hand, the stability of the detection signal of the specific protein is poor.

SUMMERY OF THE UTILITY MODEL

A first object of the utility model is to provide a blood sample analyzer, it aims at solving and adopts the nephelometry to carry out specific protein detection time measuring in the conventional art and collects the not too reasonable technical problem of angle design to the scattered light.

In order to achieve the purpose, the utility model provides a scheme is: a blood sample analyzer comprises a sampling device, a reagent supply device, a turbidimetric measurement device and a control device;

the sampling device is used for collecting a blood sample from a sample container and distributing at least part of the collected blood sample to the turbidimetric measurement device;

the reagent supply device is used for sucking a reagent from a reagent container and conveying the sucked reagent to the turbidimetric measurement device;

the turbidimetric measuring device comprises a reaction pool, a front optical assembly and a rear optical assembly, wherein the reaction pool is provided with an inner cavity for providing a reaction field for a blood sample and a reagent so as to prepare a sample, and the front optical assembly and the rear optical assembly are respectively positioned at two opposite side parts of the reaction pool;

the front light assembly is used for emitting parallel light rays towards the reaction tank;

the rear light assembly comprises a scattered light channel and a scattered light collecting component, and the scattered light channel is arranged between the reaction cell and the scattered light collecting component and is used for transmitting scattered light rays generated by the irradiation of the front light assembly on the sample to the scattered light collecting component;

the control device is in communication connection with the scattered light collecting component and is used for analyzing and calculating specific protein parameters of the blood sample according to the measurement data fed back by the scattered light collecting component;

the inner side wall of the inner cavity comprises an incident surface and an emergent surface, the incident surface faces the front light assembly and is used for transmitting the parallel light rays emitted by the front light assembly and irradiating the parallel light rays into the inner cavity, and the emergent surface faces the rear light assembly and is used for transmitting the light rays in the inner cavity and emitting the light rays out of the reaction tank towards the rear light assembly;

when the front light assembly emits the parallel light rays towards the reaction cell, a transmitted light beam is formed on the reaction cell, the transmitted light beam is provided with a first edge light beam, the first edge light beam is the light beam of the transmitted light beam with the minimum distance to the scattered light collecting component in the height direction of the reaction cell, and the incident surface is provided with a first edge line which is intersected with the first edge light beam;

when any point on the first edge line is taken as a first reference point of light emission, each light ray emitted from the first reference point can be collected by the scattered light collection part, and the scattered light collection part comprises a first initial emission light line segment, a first refraction light line segment and a second refraction light line segment, the first initial emission light line segment is a light line segment which is emitted from the first reference point and propagates in the inner cavity, the first refraction light line segment is a light line segment which is formed by irradiating the first initial emission light line segment into the reaction cell wall body and refracting, and the second refraction light line segment is a light line segment which is formed by irradiating the first refraction light line segment out of the reaction cell and refracting to the scattered light collection part through the scattered light channel;

the scattered light collection member is capable of collecting each light ray emitted from the first reference point satisfying the following condition: the first initial emission line segment forms an angle with the first edge beam that is greater than or equal to 12.3 °.

A second object of the present invention is to provide a blood sample analyzer, which comprises a sampling device, a reagent supplying device, a turbidimetric measuring device and a control device;

the turbidimetric measurement device comprises a reaction cell, a front light assembly and a rear light assembly, wherein the reaction cell is provided with an inner cavity for providing a reaction field for a blood sample and a reagent so as to prepare a sample, and the front light assembly and the rear light assembly are respectively positioned at two opposite side parts of the reaction cell;

when the front light assembly emits the parallel light rays towards the reaction cell, a transmitted light beam is formed on the reaction cell, the transmitted light beam is provided with a first edge light beam, a second edge light beam and a middle light beam, and the first edge light beam is the light beam of the transmitted light beam with the minimum distance to the scattered light collecting component in the height direction of the reaction cell;

the second edge beam is the beam of the transmitted beam with the largest distance to the scattered light collecting component in the height direction of the reaction cell, the intermediate beam is the beam with the same distance to the first edge beam and the second edge beam in the height direction of the reaction cell, the intermediate beam has a median line, and the distance of the median line to the incident surface in the propagation direction of the intermediate beam is equal to the distance to the emergent surface;

when any point on the median line is taken as a third reference point of light emission, each light ray emitted from the third reference point can be collected by the scattered light collection component, and the scattered light collection component comprises a second initial emission light ray segment, a fifth refraction light ray segment and a sixth refraction light ray segment, the second initial emission light ray segment is a light ray segment which is emitted by the third reference point and propagates in the inner cavity, the fifth refraction light ray segment is a light ray segment which is formed by irradiating the second initial emission light ray segment into the reaction cell wall body and refracting, and the sixth refraction light ray segment is a light ray segment which is formed by irradiating the fifth refraction light ray segment out of the reaction cell and refracting to the scattered light collection component through the scattered light channel;

the scattered light collection member is capable of collecting each light ray emitted from the third reference point satisfying the following condition: the second initial emission line segment makes an angle with the intermediate beam of light greater than or equal to 17.5 °.

A third object of the present invention is to provide a turbidimetric apparatus, including:

a reaction cell formed with an inner cavity for providing a reaction field for a sample and a reagent so as to prepare a sample;

the rear light assembly comprises a scattered light channel and a scattered light collecting component, and the scattered light channel is arranged between the reaction cell and the scattered light collecting component and is used for transmitting scattered light rays generated by irradiating the sample by the front light assembly to the scattered light collecting component;

the front light assembly and the rear light assembly are respectively positioned at two opposite side parts of the reaction tank, the inner side wall of the inner cavity comprises an incident surface and an emergent surface, the incident surface faces the front light assembly and is used for transmitting the parallel light rays emitted by the front light assembly and irradiating the parallel light rays into the inner cavity, and the emergent surface faces the rear light assembly and is used for transmitting the light rays in the inner cavity and emitting the light rays out of the reaction tank towards the rear light assembly;

A fourth object of the present invention is to provide a turbidimetric apparatus, include:

the second edge beam is the beam of the transmitted beam with the largest distance to the scattered light collecting component in the height direction of the reaction cell, the middle beam is the beam with the same distance to the first edge beam and the second edge beam in the height direction of the reaction cell, the middle beam has a middle line, and the distance from the middle line to the incident surface in the propagation direction of the middle beam is the same as the distance from the incident surface to the emergent surface;

the scattered light collection member is capable of collecting each light ray emitted from the third reference point so as to satisfy the following condition: the second initial emission line segment makes an angle with the intermediate beam of light greater than or equal to 17.5 °.

The utility model discloses the turbidimetric measuring device that blood sample analyzer that first purpose provided and third purpose provided, through shine preceding light subassembly to the light beam that the vertical direction in the transmitted beam that forms on the reaction cell goes up the minimum light beam of scattered light collecting element distance and define first marginal beam, and define the part of interacting with first marginal beam on the reflecting surface of reaction cell as first marginal line, use the point on the first marginal line as the light emission point to carry out the light collection angle scope of injecing scattered light collecting element simultaneously, so that scattered light collecting element can collect each light that the point emission from first marginal line all satisfies the condition that the contained angle that first initial emission line section and first marginal beam become is greater than or equal to 12.3, like this, can make the light that emits from first benchmark, if the contained angle that initial emission line section that is located in the inner chamber and first marginal beam become is less than 12.3 degrees, the light cannot be transmitted to the scattered light collecting member, so that the interference of unnecessary light can be effectively reduced, and the accuracy of the detection result can be improved. Furthermore, because the utility model discloses the boundary condition to scattered light collecting element collection angle scope foundation has carried out clear and definite definition, so, reduced by a wide margin than turbid measuring device's the design degree of difficulty.

The blood sample analyzer provided by the second object of the present invention and the turbidimetric measuring device provided by the fourth object of the present invention define the middle light beam in the vertical direction in the transmission light beam formed by irradiating the front light assembly onto the reaction cell, and define the light collection angle range of the scattered light collection member by using the point on the middle light beam middle branch as the light emission point, so that the scattered light collection member can collect each light beam emitted from the middle point on the middle branch, and the included angle formed by the second initial emission light segment and the middle light beam is greater than or equal to 17.5 degrees, thus, the light beam emitted from the third reference point can be made, if the included angle formed by the initial emission light segment and the middle light beam in the inner cavity is less than 17.5 degrees, the light beam can not be transmitted to the scattered light collection member, thereby effectively reducing the interference of unnecessary light beams, and further the accuracy of the detection result is improved. Furthermore, because the utility model discloses the boundary condition to scattered light collecting element collection angle scope foundation has carried out clear and definite definition, so, reduced by a wide margin than turbid measuring device's the design degree of difficulty.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

Fig. 1 is a schematic view illustrating a range of light collected by a scattered light collecting element according to an embodiment of the present invention;

fig. 2 is a schematic front view of a turbidimetric measuring apparatus according to an embodiment of the present invention;

fig. 3 is a schematic top view of a turbidimetric apparatus according to an embodiment of the present invention;

fig. 4 is a schematic view of a backlight assembly according to an embodiment of the present invention;

fig. 5 is a schematic view of a front light assembly according to an embodiment of the present invention;

fig. 6 is a schematic diagram illustrating a blood sample analyzer according to an embodiment of the present invention;

FIG. 7 is a schematic diagram showing the test results of embodiment 1 provided in the first embodiment of the present invention;

FIG. 8 is a schematic diagram showing the test results of embodiment 2 provided in the first embodiment of the present invention;

FIG. 9 is a schematic diagram showing the test results of embodiment 3 provided in the first embodiment of the present invention;

fig. 10 is a schematic diagram of the test results of a comparative scheme provided in the embodiment of the present invention;

fig. 11 is a schematic front view of a turbidimetric measurement apparatus according to a second embodiment of the present invention;

fig. 12 is a schematic front view of a turbidimetric measurement apparatus according to a third embodiment of the present invention;

fig. 13 is a schematic front view of a turbidimetric measurement apparatus according to the fourth embodiment of the present invention;

fig. 14 is a schematic front view of a turbidimetric measurement apparatus according to a fifth embodiment of the present invention;

fig. 15 is a schematic view of a range where light is collected by the scattered light collecting element according to a sixth embodiment of the present invention.

The reference numbers illustrate:

100. a turbidimetric measurement device; 110. a reaction tank; 111. an inner cavity; 112. an incident surface; 1121. a first edge line; 113. an exit surface; 1131. a second edge line; 120. a front light assembly; 121. a light source; 122. a second diaphragm; 123. a collimating lens; 124. a third diaphragm; 130. a backlight assembly; 131. a scattered light channel; 1311. a first inner side portion; 1312. a second inner side portion; 1313. a first light-transmitting hole; 1314. a second light-transmitting hole; 132. a scattered light collecting member; 1321. detecting a surface; 133. a first diaphragm; 134. a transmission light channel; 135. a transmitted light processing member; 136. a light absorbing member; 200. a sampling device; 300. a reagent supply device; 400. a control device; 500. a blood sedimentation measuring device; 600. a blood routine measuring device; 10. transmitting the light beam; 11. a first edge beam; 12. a second edge beam; 13. an intermediate beam; 20. a first initial emitted light segment; 21. a first refracted ray segment; 22. a second refracted ray segment; 30. a third fold line segment; 31. a fourth refracted ray segment; 40. a second initial emission light segment; 41. a fifth refracted ray segment; 42. a sixth refracted ray segment; a. a first reference point; b. a second reference point; c. and a third reference point.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

The first embodiment is as follows:

as shown in fig. 1 to 7, a blood sample analyzer according to a first embodiment of the present invention includes a sampling device 200, a reagent supply device 300, a turbidimetric measurement device 100, and a control device 400; the sampling device 200 is used for collecting and distributing blood samples, and the reagent supply device 300 is used for supplying reagents; the turbidimetric apparatus 100 is used for measuring a sample made of a sample and a reagent by nephelometry, and the control device 400 is used for analyzing and calculating parameters of a blood sample according to measurement data fed back by the turbidimetric apparatus 100.

As one embodiment, the turbidimetric apparatus 100 includes a reaction cell 110, a front light module 120 and a rear light module 130, the front light module 120 is used for emitting light toward the reaction cell 110; the backlight assembly 130 is used to collect scattered light generated by the front light assembly 120 upon the sample. The control device 400 is used for analyzing and calculating the parameters of the blood sample according to the measurement data fed back by the backlight assembly 130.

In one embodiment, the back light assembly 130 includes a scattered light channel 131 and a scattered light collecting member 132, the scattered light channel 131 is disposed between the reaction cell 110 and the scattered light collecting member 132, and the scattered light channel 131 is used for guiding scattered light generated by the front light assembly 120 irradiating the sample to the scattered light collecting member 132. The scattered light collecting member 132 is used for collecting scattered light generated by the front light assembly 120 irradiating the sample and transmitted through the scattered light channel 131, and converting the collected light signal into an electrical signal to be transmitted to the control device 400. The control device 400 is connected in communication with the scattered light collection component 132 for analyzing and calculating the specific protein parameter of the blood sample according to the measurement data fed back by the scattered light collection component 132. In this embodiment, the turbidimetric apparatus 100 is used to measure a specific protein parameter of a blood sample, but in particular applications, the turbidimetric apparatus 100 may be used to measure parameters of other samples.

In one embodiment, the front light assembly 120 and the rear light assembly 130 are respectively disposed at opposite sides of the reaction cell 110. Thus, it is advantageous to prevent the reflected light from the front light assembly 120 to the reaction cell 110 assembly from interfering with the scattered light collected by the rear light assembly 130.

In one embodiment, the reaction cell 110 is formed with a lumen 111 for providing a reaction field for the blood sample and the reagent so as to prepare a sample. The inner sidewall of the inner cavity 111 includes an incident surface 112 and an exit surface 113, the incident surface 112 faces the front light assembly 120 and is used for parallel light rays emitted by the front light assembly 120 to penetrate and irradiate into the inner cavity 111, and the exit surface 113 faces the back light assembly 130 and is used for light rays in the inner cavity 111 to penetrate and exit out of the reaction tank 110 towards the back light assembly 130. The incident surface 112 and the exit surface 113 are disposed opposite to each other. The incident surface 112 is located between the front light assembly 120 and the exit surface 113, and the exit surface 113 is located between the incident surface 112 and the rear light assembly 130.

When the front light assembly 120 emits parallel light rays toward the reaction cell 110, a transmitted light beam 10 is formed on the reaction cell 110, and the transmitted light beam 10 is a set of transmitted light rays formed by the parallel light rays emitted by the front light assembly 120 penetrating the reaction cell 110. The transmitted beam 10 has a first edge beam 11, the first edge beam 11 is the beam of the transmitted beam 10 having the smallest distance to the scattered light collecting member 132 in the height direction of the reaction cell 110, and the incident surface 112 has a first edge line 1121 intersecting with the first edge beam 11. In this embodiment, the height direction of the reaction tank 110 is a vertical direction. The first edge beam 11 is the bottom most or top most beam of the transmitted beam 10.

When any point on the first edge line 1121 is used as a first reference point a for light emission, each light emitted from the first reference point a can be collected by the scattered light collection part 132, and includes a first initial emission light segment 20, a first refraction light segment 21 and a second refraction light segment 22, the first initial emission light segment 20 is a light segment emitted from the first reference point a and propagating in the inner cavity 111, the first refraction light segment 21 is a light segment formed by refraction of the first initial emission light segment 20 irradiating into the wall of the reaction cell 110, and the second refraction light segment 22 is a light segment formed by refraction of the first refraction light segment 21 irradiating out of the reaction cell 110 and propagating to the scattered light collection part 132 through the scattered light channel 131. The scattered light collection member 132 can collect each light ray emitted from the first reference point a satisfying the following condition: the first initially emitted light segment 20 makes an angle a with the first edge beam 11 which is greater than or equal to 12.3 °. In this embodiment, a point on the first edge line 1121 is used as a light emitting point, and an included angle between a light beam emitted from the light emitting point and the first edge light beam 11 is used as a boundary condition according to the collection angle range of the scattered light collection unit 132, that is, a lower boundary condition according to the collection angle range of the scattered light collection unit 132 is clearly defined, so that the design difficulty of the rear light assembly 130 can be reduced. In addition, in the present embodiment, the first initially emitting light line segment 20 of each light ray collected by the scattered light collecting element 132 and emitted from a point on the first edge line 1121 is set to have an angle a with the first edge light beam 11 of 12.3 ° or more, so that the light ray emitted from the first reference point a cannot propagate to the scattered light collecting element 132 if the initially emitting light line segment in the inner cavity 111 has an angle with the first edge light beam 11 of less than 12.3 °, and thus the interference of unnecessary light rays can be effectively reduced, thereby improving the accuracy of the detection result.

In one embodiment, the reagent supply device 300 is used to aspirate a reagent from a reagent container and to transfer the aspirated reagent to the turbidimetric measurement device 100. The reagent supplied from the reagent supplying apparatus 300 is specifically supplied into the reaction cell 110.

In one embodiment, the turbidimetric apparatus 100 is used for performing specific protein parameter detection on a whole blood sample, and the reagent supplying device 300 is used for sucking a hemolysis reagent and transferring the hemolysis reagent to the reaction cell 110, and is used for sucking a latex reagent and transferring the latex reagent to the reaction cell 110. The whole blood sample is a blood sample directly collected from a human body or an animal without being subjected to dilution treatment. When the parameters of the specific protein are required to be measured on the basis of whole blood, because the specific protein exists in plasma, blood cells influence the signal of the turbidimetry, and thus the final detection result is influenced, so that a whole blood sample needs to be processed by using a hemolysis reagent, and the influence of the blood cells on the detection signal is eliminated. The latex reagent with the antibody can combine with the specific protein antigen in the serum to generate agglutination reaction, thereby increasing the scattering light intensity of the sample. Collecting scattered light signals within a certain angle range, and analyzing the intensity change of the scattered light to characterize the amount of specific protein in the sample. The treatment of blood cells by the hemolysis reagent is limited and in practice there will be a fraction of undissolved red blood cells or red blood cell debris, which can affect the measurement signal and thus further affect the performance of the nephelometric measurement device 100, particularly for samples with low values of a particular protein (low content of a particular protein). In this embodiment, the collection angle a in the low angle direction is limited to 12.3 °, so that the influence of non-hemolyzed cells and hemolyzed debris on the scattered light signal can be reduced, and the structure is simple and easy to implement. Of course, in a specific application, the turbidimetric apparatus 100 can also be used to perform specific protein parameter detection on serum samples obtained by centrifugation, and in this case, the reagent supplying device 300 is used to suck the latex reagent and deliver it to the reaction cell 110 without delivering the hemolysis reagent.

In one embodiment, the turbidimetric apparatus 100 is a CRP measuring apparatus or a SAA measuring apparatus. The CRP measuring device is used to measure a specific protein parameter CRP (C-reactionprotein) and the SAA measuring device is used to measure a specific protein parameter SAA (Serum amyloid a). Of course, the turbidimetric apparatus 100 may be used to measure other types of specific protein parameters in a particular application.

In one embodiment, the blood sample analyzer includes two turbidimetric measurement devices 100, one of the turbidimetric measurement devices 100 being a CRP measurement device and the other being a SAA measurement device. Of course, in particular applications, the turbidimetric apparatus 100 included in the blood sample analyzer is not limited thereto, and for example, as an alternative embodiment, two turbidimetric apparatus 100 included in the blood sample analyzer may be used to measure the same type of specific protein parameter; alternatively, as another alternative embodiment, the blood sample analyzer may include only one turbidimetric measurement device 100; alternatively, the blood sample analyzer may include three or more turbidimetric measurement devices 100 as still another alternative embodiment.

As an embodiment, the scattered light collection member 132 can collect each light ray emitted from the first reference point a satisfying the following condition: the first initially emitted light segment 20 makes an angle a with the first edge beam 11 that is greater than or equal to 15 °. In this embodiment, if the angle a between the initial emitting light segment in the inner cavity 111 and the first edge light beam 11 is smaller than 15 °, the light cannot be transmitted to the scattered light collecting element 132, so that the interference of unnecessary light can be further reduced, and the accuracy of the specific protein parameter detection result can be further improved. Of course, the minimum angle of the angle a between the first initial emission light segment 20 and the first edge light beam 11 in the boundary condition of the scattered light collection member 132 for collecting light rays is not limited to 12.3 ° and 15 °, and may be any value between 12.3 ° and 15 °, such as 12.5 °, 13 °, 13.5 °, 13.6 °, 14 °, 14.5 °, 14.6 °, and the like.

As an embodiment, the reagent supplying apparatus 300 uses a combination of a syringe and a hydraulic valve to dose the reagent; of course, in a specific application, the reagent supplying device 300 may also dose the reagent in other manners, such as a combination of a dosing pump and a hydraulic valve; or a quantification pool is adopted for quantification and the like.

In one embodiment, the transmitted light beam 10 further has a second edge beam 12, the second edge beam 12 is the light beam of the transmitted light beam 10 having the largest distance to the scattered light collecting member 132 in the height direction of the reaction cell 110, and the exit surface 113 has a second edge line 1131 intersecting with the second edge beam 12. One of the first edge beam 11 and the second edge beam 12 is the bottom-most beam of the transmitted beam 10, and the other is the top-most beam.

When any point on the second edge line 1131 is used as the second reference point b for light emission, each light ray emitted from the second reference point b can be collected by the scattered light collection member 132, and includes a third refracted light segment 30 and a fourth refracted light segment 31, the third refracted light segment 30 is a light ray segment emitted from the second reference point b and irradiated into the wall of the reaction cell 110 for refraction, and the fourth refracted light segment 31 is a light ray segment irradiated from the third refracted light segment 30 out of the reaction cell 110 for refraction and propagated to the scattered light collection member 132 through the scattered light channel 131. The scattered light collection member 132 can collect each light ray emitted from the second reference point b satisfying the following condition: the third fold line segment 30 makes an angle B with the second edge beam 12 that is less than or equal to 38 °. In this embodiment, a point on the second edge line 1131 is used as a light emitting point, and an included angle B between a light beam emitted from the light emitting point and the second edge light beam 12 is used as another boundary condition according to the collection angle range of the scattered light collection component 132, that is, an upper boundary condition according to the collection angle range of the scattered light collection component 132 is clearly defined, so that the design difficulty of the rear light assembly 130 can be reduced. In addition, in the present embodiment, the third refraction light line segment 30 of each light ray collected by the scattered light collection member 132 and emitted from a point on the second edge 1131 is set to have an angle B with the second edge beam 12 smaller than or equal to 38 °, so that the light ray emitted from the second reference point B cannot propagate to the scattered light collection member 132 if the angle B between the third refraction light line segment 30 and the second edge beam 12 is larger than 38 °, thereby effectively improving the signal-to-noise ratio and enhancing the signal stability. Since the lower limit boundary condition on which the collection angle range of the scattered light collection member 132 is based has been defined and defined as described above, in combination with the definition and definition of the upper limit boundary condition on which the collection angle range of the scattered light collection member 132 is based, the accuracy and stability of the measurement result are improved.

As an embodiment, the scattered light collection member 132 can collect each light ray emitted from the second reference point b satisfying the following condition: the third fold line segment 30 makes an angle B with the second edge beam 12 that is less than or equal to 35.5 °. In this embodiment, if the angle B formed by the third refracted light segment 30 and the second edge light beam 12 is greater than 35.5 ° with respect to the light emitted from the second reference point B, the light cannot propagate to the scattered light collecting member 132, and the stability of the detection signal can be further improved. Of course, the maximum angle of the angle formed by the third folded light line segment 30 and the second edge light beam 12 in the boundary condition of the scattered light collection member 132 for collecting light rays is not limited to 38 ° and 35.5 °, and may be any value between 35.5 ° and 38 °, such as 36 °, 36.2 °, 36.5 °, 37 °, 37.5 °, 37.8 °, and the like.

As an embodiment, the scattered light collection member 132 can collect each light ray emitted from the first reference point a satisfying the following condition: the first initial emission segment 20 makes an angle a with the first edge beam 11 of greater than or equal to 15 °, and the third emission segment 30 makes an angle B with the second edge beam 12 of less than or equal to 35.5 °. Of course, in a specific application, the boundary condition of the angular range of the light collected by the scattered light collecting member 132 is not limited to this, and for example, as an alternative embodiment, the scattered light collecting member 132 can collect each light emitted from the first reference point a satisfying the following condition: the first initial emission light segment 20 makes an angle a with the first edge beam 11 that is greater than or equal to 12.3 °, and the third emission light segment 30 makes an angle B with the second edge beam 12 that is less than or equal to 38 °; or, as another alternative embodiment, the scattered light collection member 132 can collect each light ray emitted from the first reference point a satisfying the following condition: the first initial emission light segment 20 makes an angle a with the first edge beam 11 that is greater than or equal to 12.3 °, and the third emission light segment 30 makes an angle B with the second edge beam 12 that is less than or equal to 35.5 °; still alternatively, as still another alternative embodiment, the scattered light collecting member 132 may be configured to collect each light ray emitted from the first reference point a so as to satisfy the following condition: the first initial emission segment 20 makes an angle a with the first edge beam 11 greater than or equal to 15 °, and the third emission segment 30 makes an angle B with the second edge beam 12 less than or equal to 38 °.

In one embodiment, the front light unit 120 is disposed at the left side of the reaction cell 110, the rear light unit 130 is disposed at the right side of the reaction cell 110, the incident surface 112 is the left side wall surface of the cavity 111, and the emission surface 113 is the right side wall surface of the cavity 111. Of course, in a specific application, the positions of the front light assembly 120, the back light assembly 130 and the reaction cell 110 are not limited to this, for example, as an alternative embodiment, the front light assembly 120 may be disposed at the front side of the reaction cell 110, the back light assembly 130 may be disposed at the back side of the reaction cell 110, the incident surface 112 is the front side wall surface of the cavity 111, and the emergent surface 113 is the back side wall surface of the cavity 111; alternatively, as another alternative embodiment, the front light module 120 may be disposed at the right side of the reaction cell 110, the back light module 130 may be disposed at the left side of the reaction cell 110, the incident surface 112 may be the right side wall surface of the cavity 111, and the exit surface 113 may be the left side wall surface of the cavity 111; alternatively, as still another alternative embodiment, the front light unit 120 may be provided at the rear side of the reaction cell 110, the rear light unit 130 may be provided at the front side of the reaction cell 110, the incident surface 112 may be a rear wall surface of the cavity 111, and the emission surface 113 may be a left wall surface of the cavity 111.

In one embodiment, the backlight assembly 130 further includes a first diaphragm 133, and the first diaphragm 133 is disposed in the scattered light channel 131. The first diaphragm 133 is used to block a portion of the light to prevent a portion of the scattered light generated by the front light assembly 120 and irradiated on the sample from being transmitted to the scattered light collecting part 132, so as to limit the angle range of the light collected by the scattered light collecting part 132. In theory, the limitation of the range of angles of the scattered light collected by the scattered light collecting member 132 can be achieved by changing the inclination angle of the scattered light channel 131, but this will inevitably lead to a change in the size of the turbidimetric apparatus 100.

When the light is emitted from the first reference point a, the first diaphragm 133 can prevent a part of the light emitted from the first reference point a from propagating to the scattered light collecting member 132. In particular, the first diaphragm 133 can block at least part of the light rays emitted from the first reference point a and having an angle a of less than 12.3 ° or less than 15 ° between the initial emission light segment in the cavity 111 and the first edge light beam 11.

In one embodiment, the scattered light channel 131 has a first inner portion 1311 close to the reaction cell 110 and a second inner portion 1312 disposed opposite to the first inner portion 1311 and apart from the reaction cell 110, the scattered light collecting member 132 has a detection surface 1321 directly opposite to the scattered light channel 131 for receiving scattered light, and the first diaphragm 133 is protruded on the second inner portion 1312 or the detection surface 1321.

In one embodiment, the first aperture 133 is disposed inside an end of the scattered light tunnel 131 close to the detection surface 1321. Specifically, the first diaphragm 133 abuts the second inner side portion 1312 and abuts on the detection surface 1321. With this arrangement, the first diaphragm 133 can be relatively easily installed and mounted.

When the light is emitted from the first reference point a, the first diaphragm 133 can prevent a part of the light emitted from the first reference point a from propagating to the scattered light collecting member 132. Specifically, when the first initially emitted light segment 20 of each light ray collected by the scattered light collection part 132 and emitted from a point on the first edge line 1121 is set to have an angle a with the first edge beam 11 of 12.3 ° or more, if the light ray is emitted from the first reference point a, the first diaphragm 133 can block at least part of the light ray whose initially emitted light segment has an angle with the first edge beam 11 of less than 12.3 ° so that the light ray cannot propagate to the scattered light collection part 132, whereby the interference of unnecessary light rays can be effectively reduced, and the accuracy of the detection result can be improved. By adopting the scheme of the embodiment, the collection angle A in the low-angle direction can be limited to 12.3 degrees on the premise of not increasing the size and the volume of the turbidimetric measurement device 100, so that the influence of non-hemolyzed cells and hemolyzed debris on scattered light signals can be reduced, and the device is simple in structure and easy to realize. Of course, in a specific application, when it is necessary to improve the scattered light collection angle range of a certain turbidimetric apparatus 100, the first diaphragm 133 is not limited to be provided in the scattered light tunnel 131, and for example, alternatively, it may be realized by adjusting at least one of the length of the scattered light tunnel 131, the inclination angle of the scattered light tunnel 131, the size of the detection surface 1321, and the inclination angle of the detection surface 1321.

When the first initially emitted light segment 20 of each light ray collected by the scattered light collection part 132 to be emitted from a point on the first edge line 1121 is set to have an angle a of 15 ° or more with respect to the first edge light beam 11, if the light ray is emitted from the first reference point a, the first diaphragm 133 may block at least a part of the light ray having an angle a of less than 15 ° with respect to the first edge light beam 11 so that the light ray cannot propagate to the scattered light collection part 132. By adopting the scheme of the embodiment, the collection angle A in the low-angle direction can be limited to 15 degrees on the premise of not increasing the size and the volume of the turbidimetric measurement device 100, so that the influence of non-hemolyzed cells and hemolytic debris on scattered light signals can be reduced, and the device is simple in structure and easy to realize.

In one embodiment, the backlight assembly 130 further includes a transmission light channel 134, and the transmission light channel 134 is used for guiding and transmitting the transmission light generated by the front light assembly 120 irradiating on the sample. The scattered light channel 131 is obliquely disposed below the transmission light channel 134, and the scattered light collecting member 132 is correspondingly disposed below the transmission light channel 134. The first edge beam 11 corresponds to the bottom-most beam of the transmitted beam 10, and the first edge line 1121 corresponds to the lower edge line of the incident surface 112 intersecting the first edge beam 11.

In one embodiment, the scattering light channel 131 and the transmission light channel 134 form an inclined angle C of 30.8 ° to 41.3 °, and the inclined angle C may be an angle between two adjacent outer sidewalls of the scattering light channel 131 and the transmission light channel 134, or an angle between a center line of the scattering light channel 131 and a center line of the transmission light channel 134. Here, by limiting the inclined angle C between the scattered light channel 131 and the transmitted light channel 134, the structural design of the turbidimetric measurement apparatus 100 can be made more reasonable and the size can be made not too large on the premise of satisfying the collection angle range. Of course, the inclined angle between the scattered light channel 131 and the transmitted light channel 134 is not limited to this in specific applications.

In one embodiment, the inclined angle D between the scattered light collecting member 132 and the height direction of the reaction cell 110 is 28.1 ° to 30.8 °, that is, the inclined angle D between the scattered light collecting member 132 and the vertical direction is 28.1 ° to 30.8 °. The inclined angle D formed by the scattered light collection member 132 and the vertical direction is specifically the angle formed by the detection surface 1321 and the vertical direction. Here, by limiting the inclination angle of the detection surface 1321, the structural design of the turbidimetric measurement apparatus 100 can be made reasonable and the body size can be made not too large on the premise that the collection angle range is satisfied. Of course, the angle of inclination of the detection surface 1321 is not limited thereto in specific applications.

In one embodiment, the transmitted light channel 134 and the scattered light channel 131 are integrally formed on the same component, for example, the transmitted light channel 134 and the scattered light channel 131 are both disposed on a pressing block fixed on the outer side of the reaction cell 110, which is beneficial to reducing the number of components and ensuring the processing precision of the inclination angle between the transmitted light channel 134 and the scattered light channel 131. Of course, in certain applications, the transmitted light channel 134 and the scattered light channel 131 may be formed separately from two different components, as an alternative embodiment.

In one embodiment, the backlight assembly 130 further includes a transmitted light processing part 135, and a transmitted light channel 134 is disposed between the reaction cell 110 and the transmitted light processing part 135 for guiding the transmitted light generated by the front light assembly 120 irradiating the sample to the transmitted light processing part 135. The transmitted light processing component 135 is configured to absorb transmitted light from the front light assembly 120 that impinges on the sample and/or reflect transmitted light from the front light assembly 120 that impinges on the sample out of the transmitted light channel 134. The transmitted light processing part 135 is provided mainly to prevent the transmitted light from being reflected back to the reaction cell 110, thereby preventing the transmitted light from affecting the measurement result.

In one embodiment, the transmission light processing unit 135 is disposed at an end of the transmission light channel 134 away from the reaction cell 110. The transmission light processing component 135 and the transmission light channel 134 are arranged in a non-perpendicular mode, transmission light rays are emitted to the absorption surface of the transmission light processing component 135 in a non-perpendicular angle, a part of the transmission light rays are absorbed by the transmission light processing component 135, the other part of unabsorbed light rays are reflected by the absorption surface, and an included angle is formed between the absorption surface of the transmission light processing component 135 and the transmission light rays, so that an included angle is formed between a reflected path and a path emitted to the absorption surface of the transmission light processing component 135, the reflected light rays cannot return along the transmission light channel 134, and the measurement accuracy is further improved.

In one embodiment, the inner sidewall of the transmission light channel 134 is provided with a light absorbing member 136, the transmission light processing member 135 can absorb the transmission light transmitted through the transmission light channel 134, and can secondarily absorb the transmission light which is not absorbed by the light absorbing member 136 reflected to the inner sidewall of the transmission light channel 134, and the light absorbing member 136 of the inner sidewall of the transmission light channel 134 can be a black machined member or other member with better light absorbing property. Of course, in a specific application, the light absorbing member 136 may not be provided, for example, a light hole may be provided instead, so that the light reflected by the transmitted light processing member 135 can be emitted out of the transmitted light channel 134.

In one embodiment, the transmitted light processing component 135 is a neutral density sheet that absorbs light and reflects unabsorbed light to a designated location. Of course, the transmissive light processing component 135 may be configured as other components with better light absorption or reflection properties for specific applications.

In one embodiment, the front light assembly 120 includes a light source 121, a second diaphragm 122, a collimating lens 123 and a third diaphragm 124, and the second diaphragm 122, the collimating lens 123 and the third diaphragm 124 are sequentially disposed between the laser and the reaction cell 110. When the turbidimetric apparatus 100 is in operation, a light beam emitted by the light source 121 irradiates the second diaphragm 122, the second diaphragm 122 is used for limiting the size of the light beam incident on the collimating lens 123, and then the light beam exits through the third diaphragm 124, and the third diaphragm 124 is used for limiting the size of the light beam irradiating the reaction cell 110.

As an embodiment, the front surface of the collimating lens 123 close to the light source 121 is designed as a curved surface, and the curved surface is coated with a reflection reducing film, so that the reflectivity can be controlled to be less than 2.5 ‰, and the feedback light reflected back to the light source 121 by the collimating lens 123 is effectively prevented from affecting the stable output of the light source 121.

As an embodiment, the light source 121 employs a laser. Of course, the type of light source 121 is not limited thereto in a particular application.

In one embodiment, the output power of the laser is less than or equal to 0.2 mw, which can prolong the service life of the laser and reduce the feedback of other components, thereby improving the stability of the laser power and making the detection limit lower than that of the turbidity measuring apparatus 100. The detection limit of the turbidimetric measurement apparatus 100 refers to the detection capability of a sample having a low specific protein content, and may be referred to as a detection limit. By increasing the stability of the laser output, the detection noise can be reduced, so that a valid signal previously buried in the noise can be detected, thereby improving the detection capability of the turbidimetric measurement apparatus 100 for low-value samples (i.e., samples with low specific protein content).

In one embodiment, the third aperture 124 has a clear aperture of 1.5mm to 3.2mm and a thickness greater than 5 mm. Of course, the size parameter of the third diaphragm 124 is not limited thereto in specific applications.

In one embodiment, in order to further reduce the influence of the feedback of the returned light passing through the reaction cell 110 on the power stability of the laser, the reaction cell 110 is offset, that is, the surface of the reaction cell 110 facing the front light assembly 120 is set to deviate from the direction perpendicular to the light irradiated by the front light assembly 120 onto the reaction cell 110, so that the surface of the reaction cell 110 facing the front light assembly 120 is set to be not perpendicular to the light irradiated by the front light assembly 120 onto the reaction cell 110. The included angle between the parallel light irradiated to the reaction cell 110 by the front light assembly 120 and the outer sidewall of the reaction cell 110 is not a right angle.

In order to adapt to the offset setting of the reaction cell 110, one surface of the third diaphragm 124 facing the reaction cell 110 is a first inclined surface; one surface of the pressing block facing the reaction tank 110 is a second inclined surface, and the inclined directions of the first inclined surface and the second inclined surface are the same; the first inclined surface and the second inclined surface are disposed non-perpendicularly with respect to the light beam emitted from the light source 121. Through the arrangement, the structure of the reaction tank 110 does not need to be changed, and only the setting position of the reaction tank 110 needs to be adjusted, so that the offset adjustment operation of the reaction tank 110 can be realized by setting the reaction tank 110 between the third diaphragm 124 and the pressing block.

As an embodiment, the offset angle of the reaction tank 110 is 7 °; of course, the offset angle of the reaction cell 110 can be set to other angles for specific applications.

As an embodiment, the sampling device 200 is used to collect a blood sample from within a sample container and to dispense at least a portion of the collected blood sample to the turbidimetric measurement device 100. The reaction cell 110 of the turbidimetric measurement device 100 is used for receiving the blood sample dispensed by the sampling device 200 and for performing specific protein detection on the blood sample.

As an embodiment, the blood sample analyzer further comprises a blood sedimentation measuring device 500, the blood sedimentation measuring device 500 being configured to measure a blood sedimentation rate of the blood sample. The blood sedimentation device 500 is particularly useful for testing the dispensed blood sample collected by the sampling device 200. In this embodiment, the blood sample analyzer integrates a blood sedimentation measurement function and a specific protein measurement function; of course, in certain applications, the blood sample analyzer may not include the blood sedimentation device 500.

As an embodiment, the blood sample analyzer further comprises a blood routine measuring device 600, and the sampling device 200 is further configured to distribute at least a portion of the collected blood sample to the blood routine measuring device 600. The blood routine testing device is used for receiving the blood sample dispensed by the sampling device 200 and performing blood routine testing on the blood sample. Blood routine tests may include, but are not limited to, differential white blood cell count tests, reticulocyte tests, hemoglobin tests, red blood cell count tests, platelet count tests, and the like. In this embodiment, the blood sample analyzer integrates a blood routine measurement function and a specific protein measurement function; of course, in certain applications, the blood sample analyzer may not include the blood routine measuring device 600.

Specifically, the blood routine measuring device 600 includes at least one of an optical measuring component for measuring a white blood cell parameter and/or a reticulocyte parameter of the blood sample, a hemoglobin measuring component for detecting a hemoglobin parameter of the blood sample, and an impedance measuring component; the impedance measurement assembly is used to detect a red blood cell parameter and/or a platelet parameter of the blood sample.

The present embodiment further provides a turbidimetric apparatus 100, wherein the turbidimetric apparatus 100 comprises a reaction cell 110, a front light module 120 and a rear light module 130, the reaction cell 110 is formed with an inner cavity 111 for providing a reaction field for a sample and a reagent so as to prepare a sample, and the front light module 120 and the rear light module 130 are respectively located at two opposite side portions of the reaction cell 110. The front light assembly 120 serves to emit parallel light toward the reaction cell 110. The back light assembly 130 includes a scattered light channel 131 and a scattered light collecting member 132, and the scattered light channel 131 is disposed between the reaction cell 110 and the scattered light collecting member 132 to guide scattered light generated by the front light assembly 120 irradiating the sample to the scattered light collecting member 132. The inner sidewall of the inner cavity 111 includes an incident surface 112 and an exit surface 113, the incident surface 112 faces the front light assembly 120 and is used for parallel light rays emitted by the front light assembly 120 to penetrate and irradiate into the inner cavity 111, and the exit surface 113 faces the back light assembly 130 and is used for light rays in the inner cavity 111 to penetrate and exit out of the reaction tank 110 towards the back light assembly 130. When the front light assembly 120 emits parallel light rays toward the reaction cell 110, a transmitted light beam 10 is formed on the reaction cell 110, the transmitted light beam 10 has a first edge light beam 11, the first edge light beam 11 is a light beam of the transmitted light beam 10 with the smallest distance to the scattered light collecting part 132 in the height direction of the reaction cell 110, and the incident surface 112 has a first edge line 1121 intersecting with the first edge light beam 11. When any point on the first edge line 1121 is used as a first reference point a for light emission, each light emitted from the first reference point a by the scattered light collection part 132 includes a first primary emitted light segment 20, a first refracted light segment 21 and a second refracted light segment 22, the first primary emitted light segment 20 is a light segment emitted from the first reference point a and propagating in the inner cavity 111, the first refracted light segment 21 is a light segment formed by refraction of the first primary emitted light segment 20 irradiating into the wall of the reaction cell 110, and the second refracted light segment 22 is a light segment formed by refraction of the first refracted light segment 21 irradiating out of the reaction cell 110 and propagating to the scattered light collection part 132 through the scattered light channel 131. The scattered light collecting member 132 can collect each light ray emitted from the first reference point a satisfying the following condition: the first initially emitted light segment 20 makes an angle with the first edge beam 11 that is greater than or equal to 12.3 °. The operation principle and other parts of the turbidimetric apparatus 100 can be referred to the description of the turbidimetric apparatus 100 in the blood sample analyzer, and will not be described in detail herein.

In the embodiment, the boundary conditions of the upper limit and the lower limit of the scattered light collection range are defined and limited mainly aiming at the influence of blood cells and hemolytic debris on nephelometry measurement, wherein the lower limit of angle collection is increased to 12.3-15 degrees, so that the interference of the blood cells and hemolytic debris on the parameter detection of the specific protein can be reduced, and the detection limit of the nephelometry measurement of the specific protein is reduced; the upper limit of angle collection is improved to 35.5-38 degrees so as to improve the signal-to-noise ratio of the signal and further improve the stability of the signal. In addition, in one embodiment, the first diaphragm 133 is arranged in the scattered light channel 131 to optimize the scattering collection angle, so that the structure is simple and effective, and the purpose of improving the upper limit and the lower limit of the scattering collection angle and not changing the whole volume of the turbidimetric measurement device 100 is achieved.

The applicant has made tests using a turbidimetric apparatus 100 of the following scheme:

embodiment 1: in the turbidimetric apparatus 100 according to the present embodiment, the scattered light collection member 132 can collect each light ray emitted from the first reference point a so as to satisfy the following condition: the first initial emitted light segment 20 makes an angle a with the first edge beam 11 of greater than or equal to 15 °, and the third reflected light segment 30 makes an angle B with the second edge beam 12 of less than or equal to 38 °. The turbidimetric measurement device 100 provided in this embodiment is used to perform three specific protein measurements on the same whole blood sample with high-value RBC and low-value CRP, and three measurement curves as shown in fig. 7 are obtained, wherein the CRP value is calculated by subtracting the light intensity at the starting point from the end point of the measurement curve.

Embodiment 2: in the turbidimetric apparatus 100 according to the present embodiment, the scattered light collection member 132 can collect each light ray emitted from the first reference point a so as to satisfy the following condition: the first initial emission segment 20 makes an angle a with the first edge beam 11 that is greater than or equal to 12.3 °, and the third emission segment 30 makes an angle B with the second edge beam 12 that is less than or equal to 35.5 °. The turbidimetric measurement apparatus 100 provided in this embodiment is used to perform three specific protein measurements on the same whole blood sample with high RBC and low CRP, and three measurement curves as shown in fig. 8 are obtained.

Embodiment 3: in the turbidimetric apparatus 100 according to the present embodiment, the scattered light collection member 132 can collect each light ray emitted from the first reference point a so as to satisfy the following condition: the first initial emission light segment 20 makes an angle a with the first edge beam 11 that is greater than or equal to 13.6 °, and the third emission light segment 30 makes an angle B with the second edge beam 12 that is less than or equal to 33.3 °. The turbidimetric measurement apparatus 100 provided in this embodiment is used to perform three specific protein measurements on the same whole blood sample with high RBC and low CRP, and three measurement curves as shown in fig. 9 are obtained.

The comparison scheme comprises the following steps: in the turbidimetric apparatus 100 according to the present embodiment, the scattered light collection member 132 can collect each light ray emitted from the first reference point a so as to satisfy the following condition: the first initial emission segment 20 makes an angle a with the first edge beam 11 that is greater than or equal to 9.7 °, and the third emission segment 30 makes an angle B with the second edge beam 12 that is less than or equal to 33.3 °. The turbidimetric measurement apparatus 100 provided in this embodiment is used to perform three specific protein measurements on the same whole blood sample with high RBC and low CRP values, and three measurement curves as shown in fig. 10 are obtained.

Referring to fig. 7 to 10, it can be seen that, in the comparison scheme, due to the influence of blood cells and hemolytic debris, the front segment of the measurement curve of the comparison scheme is tilted, so as to cause an erroneous light intensity calculation, and the optimized

embodiments

1, 2, and 3 can significantly improve the interference, thereby achieving the purpose of improving the detection accuracy and stability.

Example two:

referring to fig. 2 and 11, the blood sample analyzer and turbidimetric apparatus 100 according to the present embodiment differs from the first embodiment mainly in that the first diaphragm 133 is disposed at a different position, specifically in that: in the first embodiment, the first diaphragm 133 is disposed at the outlet end of the scattered light channel 131; in this embodiment, the first diaphragm 133 is disposed at the entrance end of the scattered light channel 131.

In addition to the above differences, the blood sample analyzer and other parts of the turbidimetric apparatus 100 provided in the present embodiment may be designed in a corresponding optimization manner with reference to the present embodiment, and will not be described in detail herein.

Example three:

referring to fig. 2, 11 and 12, the blood sample analyzer and turbidimetry apparatus 100 according to the present embodiment differs from the first embodiment mainly in that the first diaphragm 133 is disposed at a different position, specifically in that: in the first embodiment, the first diaphragm 133 is disposed at the outlet end of the scattered light channel 131; in the second embodiment, the first diaphragm 133 is disposed at the entrance end of the scattered light channel 131; in this embodiment, the first diaphragm 133 is disposed between the exit end and the entrance end of the scattered light channel 131.

Example four:

referring to fig. 1, 2, and 13, the blood sample analyzer and turbidimetric apparatus 100 according to the present embodiment differs from the first embodiment mainly in the means for limiting the scattered light collection range, which are specifically embodied as: in the first embodiment, the scattered light collection range is limited by disposing a diaphragm in the scattered light channel 131; in this embodiment, the stepped inner hole of the scattered light channel 131 is used to define the scattered light collection range.

Specifically, in the present embodiment, the scattered light channel 131 includes a first light hole 1313 and a second light hole 1314, the first light hole 1313 is located between the reaction cell 110 and the second light hole 1314, and the aperture of the first light hole 1313 is smaller than that of the second light hole 1314. The inner wall of the first light-transmitting hole 1313 is used to prevent part of scattered light generated by the front light assembly 120 irradiating the sample from being transmitted to the scattered light collection member 132;

when light is emitted from the first reference point a, the inner wall of the first light-transmitting hole 1313 can prevent part of the light emitted from the first reference point a from propagating to the scattered light collecting member 132. Specifically, when the first initially emitted light segment 20 of each light ray collected by the scattered light collection member 132 and emitted from a point on the first edge line 1121 is set to have an angle a with the first edge beam 11 of 12.3 ° or more, if the light ray is emitted from the first reference point a, the inner wall of the first light transmission hole 1313 may block at least part of the light ray having an angle a with the first edge beam 11 of less than 12.3 ° so that the light ray cannot propagate to the scattered light collection member 132, and thus interference of unnecessary light rays may be effectively reduced, and the accuracy of the detection result may be improved.

When the first initially emitted light segment 20 of each light ray collected by the scattered light collection member 132 and emitted from a point on the first edge wire 1121 is set to have an angle a of 15 ° or more with respect to the first edge beam 11, if the light ray is emitted from the first reference point a, the inner wall of the first light transmission hole 1313 may shield at least part of the light ray having an angle a of 15 ° or less with respect to the first edge beam 11, so that the light ray cannot propagate to the scattered light collection member 132.

When light is emitted from the third reference point c, the inner wall of the first light-transmitting hole 1313 can prevent part of the light emitted from the third reference point c from propagating to the scattered light collecting member 132.

The same effect as that achieved in the first embodiment is that, with the solution of the present embodiment, the collection angle a in the low angle direction can be limited to 12.3 ° to 15 ° without increasing the size and volume of the turbidimetric apparatus 100, so that the influence of non-hemolyzed cells and hemolyzed debris on the scattered light signal can be reduced, and the structure is simple and easy to implement.

Example five:

referring to fig. 1, 2, and 14, the blood sample analyzer and turbidimetric apparatus 100 according to the present embodiment differs from the first embodiment mainly in the positions of the scattered light channel 131 and the scattered light collecting member 132, which are specifically embodied as: in the first embodiment, the scattered light channel 131 and the scattered light collecting member 132 are disposed below the transmitted light channel 134; in this embodiment, the scattered light channel 131 and the scattered light collecting member 132 are provided above the transmission light channel 134.

Specifically, in the present embodiment, the scattered light channel 131 is disposed obliquely above the transmitted light channel 134.

In addition to the above differences, the blood sample analyzer and other parts of the turbidimetric apparatus 100 according to the present embodiment may be optimized according to any one of the first to fourth embodiments, and will not be described in detail herein.

Example six:

referring to fig. 1, 2 and 15, the blood sample analyzer and turbidimetric apparatus 100 according to the present embodiment differs from the first embodiment mainly in the manner of defining the boundary condition of the scattered light collection range.

Specifically, the transmitted beam 10 further has an intermediate beam 13, the intermediate beam 13 is a beam having a distance from the first edge beam 11 to the second edge beam 12 in the height direction of the reaction cell 110 equal to the distance from the second edge beam 12, and the intermediate beam 13 has a median line having a distance from the incident surface 112 to the exit surface 113 in the propagation direction of the intermediate beam 13 equal to the distance from the exit surface 113. When any point on the median line is taken as the third reference point c for light emission, each light ray emitted from the third reference point c and collected by the scattered light collection member 132 includes a second initial emission light line segment 40, a fifth refraction light line segment 41 and a sixth refraction light line segment 42, the second initial emission light line segment 40 is a light line segment emitted from the third reference point c and propagating in the inner cavity 111, the fifth refraction light line segment 41 is a light line segment formed by refraction of the second initial emission light line segment 40 irradiating into the wall of the reaction cell 110, and the sixth refraction light line segment 42 is a light line segment formed by refraction of the fifth refraction light line segment 41 irradiating out of the reaction cell 110 and propagating to the scattered light collection member 132 through the scattered light channel 131. The scattered light collecting member 132 can collect each light ray emitted from the third reference point c satisfying the following condition: the second initially emitted light segment 40 makes an angle E with the intermediate light beam 13 which is greater than or equal to 17.5 °. The present embodiment corresponds to another way of clearly defining and limiting the lower boundary condition according to which the scattered light collection unit 132 collects the angular range, relative to the first embodiment of emitting light from the first reference point a, wherein the angle E between the second initially emitting light line segment 40 and the intermediate light beam 13 is greater than or equal to 17.5 °, and the angle a between the first initially emitting light line segment 20 and the first edge light beam 11 defined in the first embodiment is greater than or equal to 12.3 °, which are consistent with each other and will not be described in detail herein.

As an embodiment, the scattered light collection member 132 can collect each light ray emitted from the third reference point c satisfying the following condition: the second initially emitted light segment 40 makes an angle F with the intermediate light beam 13 smaller than or equal to 32.5 °. In contrast to the first embodiment, in which the light is emitted from the second reference point B, the present embodiment is equivalent to another way of clearly defining and limiting the upper limit boundary condition according to which the collection angle range of the scattered light collection member 132 is defined, and the effect is achieved that the defined angle F between the second initial emission light line segment 40 and the intermediate light beam 13 is less than or equal to 32.5 °, and the defined angle B between the third reflection light line segment 30 and the second edge light beam 12 is less than or equal to 38 °, which are not described in detail herein.

As an embodiment, the scattered light collection member 132 can collect each light ray emitted from the third reference point c satisfying the following condition: the second initially emitted light segment 40 makes an angle F with the intermediate light beam 13 smaller than or equal to 29.6 °. With respect to the first embodiment, in which the light is emitted from the second reference point b, the angle between the second initial emitting light line segment 40 and the intermediate light beam 13 is less than or equal to 29.6 °, and the angle between the third emitting light line segment 30 and the second edge light beam 12 is less than or equal to 35.5 °, which are the same as the first embodiment, and will not be described in detail herein.

As an embodiment, the scattered light collecting member 132 can collect each light ray emitted from the third reference point c satisfying the following condition: the second initially emitted light segment 40 makes an angle E with the intermediate light beam 13 which is greater than or equal to 20.5 °. The effect achieved by the second initially emitted light segment 40 defined in this embodiment at an angle E greater than or equal to 20.5 ° with respect to the above-described emission of light from the first reference point a, and at an angle E greater than or equal to 15 ° with respect to the above-described first initially emitted light segment 20 defined with respect to the first edge light beam 11, is the same and will not be described in detail here.

The upper limit boundary condition of the collection angle range of the scattered light collection member 132 defined by the emission light from the third reference point c is not limited to 29.6 ° and 32.2 °, and may be any value between 29.6 ° and 32.2 °. Correspondingly, the lower limit boundary condition of the collection angle range of the scattered light collection member 132 defined by the emission light from the third reference point c is not limited to 17.5 ° and 20.5 °, and may be any value between 17.5 ° and 20.5 °.

When the light is emitted from the third reference point c, the first diaphragm 133 can prevent a part of the light emitted from the third reference point c from propagating to the scattered light collecting member 132. Specifically, when the second initially emitted light segment 40 collected by the scattered light collection member 132 to each light emitted from the third reference point c is set to have an angle E of 17.5 ° or more with respect to the intermediate light beam 13, if the light emitted from the third reference point c is emitted, the first diaphragm 133 may block at least part of the light having the initially emitted light segment at an angle of less than 17.5 ° with respect to the intermediate light beam 13 so that the light cannot propagate to the scattered light collection member 132.

When the second initially emitted light segment 40 collected by the scattered light collection member 132 to each light emitted from the third reference point c is set to have an angle E of 20.5 ° or more with respect to the intermediate light beam 13, if the light emitted from the third reference point c is emitted, the first diaphragm 133 may block at least a part of the light having the initially emitted light segment with respect to the intermediate light beam 13 by an angle of less than 20.5 ° so that the light cannot propagate to the scattered light collection member 132.

It should be noted that, the boundary condition manner of the scattered light collection range in the present embodiment may also be combined with the embodiment, and the actual collection range of the scattered light obtained by the design is the same.

In addition to the above differences, the blood sample analyzer and other parts of the turbidimetric apparatus 100 according to the present embodiment may be optimized according to any one of the first to fifth embodiments, and will not be described in detail herein.

The above only be the preferred embodiment of the utility model discloses a not consequently restriction the utility model discloses a patent range, all are in the utility model discloses a conceive, utilize the equivalent structure transform of what the content was done in the description and the attached drawing, or direct/indirect application all is included in other relevant technical field the utility model discloses a patent protection within range.

Claims

1. A blood sample analyzer, characterized in that: comprises a sampling device, a reagent supply device, a turbidimetric measurement device and a control device;

the inner side wall of the inner cavity comprises an incident surface and an emergent surface, the incident surface faces the front light assembly and is used for allowing the parallel light rays emitted by the front light assembly to penetrate and irradiate into the inner cavity, and the emergent surface faces the rear light assembly and is used for allowing the light rays in the inner cavity to penetrate and emit out of the reaction tank towards the rear light assembly;

2. The blood sample analyzer of claim 1, wherein: the transmitted light beam also has a second edge light beam, the second edge light beam is the light beam of the transmitted light beam with the largest distance to the scattered light collecting component in the height direction of the reaction cell, and the emergent surface is provided with a second edge line which is intersected with the second edge light beam;

when any point on the second edge line is taken as a second reference point of light emission, each light ray emitted from the second reference point can be collected by the scattered light collection part, and the scattered light collection part comprises a third refraction light ray segment and a fourth refraction light ray segment, wherein the third refraction light ray segment is a light ray segment which is emitted by the second reference point and irradiated into the reaction cell wall body to be refracted, and the fourth refraction light ray segment is a light ray segment which is irradiated out of the reaction cell by the third refraction light ray segment to be refracted and propagated to the scattered light collection part through the scattered light channel;

the scattered light collection member is capable of collecting each light ray emitted from the second reference point so as to satisfy the following condition: the third refraction light line segment forms an included angle with the second edge light beam which is smaller than or equal to 38 degrees.

3. The blood sample analyzer of claim 2, wherein: the scattered light collection member is capable of collecting each light ray emitted from the second reference point satisfying the following condition: the included angle formed by the third refraction light line segment and the second edge light beam is less than or equal to 35.5 degrees.

4. The blood sample analyzer of claim 1, wherein: the scattered light collection member is capable of collecting each light ray emitted from the first reference point satisfying the following condition: the included angle between the first initial emitting light line segment and the first edge light beam is greater than or equal to 15 degrees.

5. The blood sample analyzer of claim 1, wherein: the transmitted light beam also has a second edge light beam and an intermediate light beam, the second edge light beam is the light beam of the transmitted light beam with the largest distance to the scattered light collecting component in the height direction of the reaction cell, the intermediate light beam is the light beam with the same distance to the first edge light beam and the second edge light beam in the height direction of the reaction cell, the intermediate light beam has a median line, and the distance of the median line to the incident surface in the propagation direction of the intermediate light beam is the same as the distance to the emergent surface;

6. A blood sample analyzer, characterized in that: comprises a sampling device, a reagent supply device, a turbidimetric measurement device and a control device;

7. The blood sample analyzer of claim 5 or 6, wherein: the scattered light collection member is capable of collecting each light ray emitted from the third reference point satisfying the following condition: the second initial emission line segment makes an angle with the intermediate beam of light less than or equal to 32.5 °.

8. The blood sample analyzer of claim 7, wherein: the scattered light collection member is capable of collecting each light ray emitted from the third reference point satisfying the following condition: the second initial emission line segment makes an angle with the intermediate beam of light less than or equal to 29.6 °.

9. The blood sample analyzer of claim 5 or 6, wherein: the scattered light collection member is capable of collecting each light ray emitted from the third reference point satisfying the following condition: the second initial emission line segment makes an angle with the intermediate beam of light greater than or equal to 20.5 °.

10. The blood sample analyzer of any one of claims 1 to 6, wherein: the rear light assembly further comprises a first diaphragm, and the first diaphragm is arranged in the scattered light channel and used for preventing part of scattered light generated by the irradiation of the front light assembly on the sample from being transmitted to the scattered light collecting component.

11. The blood sample analyzer of claim 10, wherein: the scattered light channel has and is close to the first inside portion of reaction cell and with first inside portion sets up relatively and keeps away from the second inside portion of reaction cell, scattered light collecting element have with scattered light channel just relatively is used for receiving scattered light's detection face, first diaphragm is protruding to be located the second inside portion or on the detection face.

12. The blood sample analyzer of any one of claims 1 to 6, wherein: the scattered light passageway includes first light trap and second light trap, first light trap be located the reaction cell with between the second light trap, the aperture of first light trap is less than the aperture of second light trap, just the inner wall of first light trap is used for preventing the part by preceding light subassembly shines the scattered light that produces on the sample propagates to on the scattered light collecting element.

13. The blood sample analyzer of any one of claims 1 to 6, wherein: the rear light assembly also comprises a transmission light channel which is used for guiding and transmitting transmission light rays generated by the front light assembly irradiating the sample;

the scattered light channel is obliquely arranged below or above the transmitted light channel.

14. The blood sample analyzer of claim 13, wherein: the transmission light channel is arranged between the reaction cell and the transmission light processing component and is used for transmitting transmission light rays generated by the irradiation of the front light assembly on the sample to the transmission light processing component;

the transmitted light processing component is used for absorbing the transmitted light generated by the front light assembly irradiating the sample and/or reflecting the transmitted light generated by the front light assembly irradiating the sample out of the transmitted light channel.

15. The blood sample analyzer of claim 13 wherein: the scattering light channel and the transmission light channel form an inclined included angle of 30.8-41.3 degrees; and/or the presence of a gas in the atmosphere,

the scattered light collecting component and the height direction of the reaction cell form an inclined included angle of 28.1-30.8 degrees.

16. The blood sample analyzer of any one of claims 1 to 6, wherein: the reagent supply device is used for sucking a hemolysis reagent and conveying the hemolysis reagent to the reaction tank and sucking a latex reagent and conveying the latex reagent to the reaction tank; and/or the presence of a gas in the atmosphere,

the turbidimetric measurement device is a CRP measurement device or an SAA measurement device.

17. A turbidimetric measurement device, characterized by: the method comprises the following steps:

18. The turbidimetric apparatus of claim 17, wherein:

the transmitted light beam also has a second edge light beam, the second edge light beam is the light beam of the transmitted light beam with the largest distance to the scattered light collecting component in the height direction of the reaction cell, and the emergent surface is provided with a second edge line which is intersected with the second edge light beam;

the scattered light collection member is capable of collecting each light ray emitted from the second reference point satisfying the following condition: the third refraction light line segment forms an included angle with the second edge light beam which is smaller than or equal to 38 degrees.

19. The turbidimetric apparatus of claim 18, wherein: the scattered light collection member is capable of collecting each light ray emitted from the second reference point satisfying the following condition: the included angle formed by the third refraction light line segment and the second edge light beam is less than or equal to 35.5 degrees.

20. The turbidimetric apparatus of claim 17, wherein: the scattered light collection member is capable of collecting each light ray emitted from the first reference point satisfying the following condition: the first initial emission line segment forms an angle with the first edge beam that is greater than or equal to 15 °.

21. The turbidimetric apparatus of claim 17, wherein: the transmitted light beam further has a second edge light beam and an intermediate light beam, the second edge light beam is the light beam of the transmitted light beam with the largest distance to the scattered light collecting component in the height direction of the reaction cell, the intermediate light beam is the light beam with the same distance to the first edge light beam and the second edge light beam in the height direction of the reaction cell, the intermediate light beam has a median line, and the distance to the incident surface of the median line in the propagation direction of the intermediate light beam is the same as the distance to the emergent surface of the median line;

22. A turbidimetric measurement device, characterized by: the method comprises the following steps:

23. The turbidimetric measurement device of claim 21 or 22, wherein: the scattered light collection member is capable of collecting each light ray emitted from the third reference point so as to satisfy the following condition: the second initial emission line segment makes an angle with the intermediate beam of light less than or equal to 32.5 °.

24. The turbidimetric apparatus of claim 23, wherein: the scattered light collection member is capable of collecting each light ray emitted from the third reference point satisfying the following condition: the second initial emission light segment makes an angle with the intermediate light beam smaller than or equal to 29.6 degrees.

25. The turbidimetric measurement device of claim 21 or 22, wherein: the scattered light collection member is capable of collecting each light ray emitted from the third reference point satisfying the following condition: the second initial emission line segment makes an angle with the intermediate beam of light greater than or equal to 20.5 °.

26. The turbidimetric measurement device of any of claims 17 to 22, characterized by: the rear light assembly further comprises a first diaphragm, and the first diaphragm is arranged in the scattered light channel and used for preventing part of scattered light generated by the front light assembly irradiating the sample from being transmitted to the scattered light collecting component; alternatively, the first and second electrodes may be,

the scattered light passageway includes first light trap and second light trap, first light trap is located the reaction cell with between the second light trap, the aperture of first light trap is less than the aperture of second light trap, just the inner wall of first light trap is used for preventing the part by preceding light subassembly shines the scattered light that produces on the sample propagates to on the scattered light collecting element.