CN217324059U - Efficient valine semicontinuous fermentation cylinder and complete sets - Google Patents
Efficient valine semicontinuous fermentation cylinder and complete sets Download PDFInfo
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- CN217324059U CN217324059U CN202122161403.2U CN202122161403U CN217324059U CN 217324059 U CN217324059 U CN 217324059U CN 202122161403 U CN202122161403 U CN 202122161403U CN 217324059 U CN217324059 U CN 217324059U
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Abstract
The utility model relates to an efficient valine semicontinuous fermentation cylinder and integrated equipment, including intake pipe, inlet pipe, discharging pipe, agitating unit, drive arrangement, observation window, the inlet pipe sets up at the top of fermentation cylinder, and the body length of inlet pipe in the fermentation cylinder is 1/6-1/4 of fermentation cylinder length, the discharging pipe sets up at the fermentation cylinder top, and the body length of discharging pipe in the fermentation cylinder is 1/6-1/5 of fermentation cylinder length, drive arrangement sets up at the top of fermentation cylinder and connects agitating unit, agitating unit extends to the fermentation cylinder bottom from the fermentation cylinder top, is provided with the multiunit stirring rake on the agitating unit; and the tail end of the air inlet pipe is provided with an air outlet which is arranged under the stirring device. The beneficial effects of the utility model reside in that: the fermentation tank and the complete equipment suitable for producing valine by semi-continuous fermentation are provided, the fermentation process of valine can be simplified, the fermentation period is shortened, and the post-treatment difficulty is reduced.
Description
Technical Field
The utility model relates to a bioengineering technical field, concretely relates to efficient valine semicontinuous fermentation cylinder and integrated equipment.
Background
Valine is an essential amino acid and a nutrient substance for organisms, is widely applied to the fields of foods, medicines, cosmetics, feeds and the like, is widely used as a food additive, a feed additive, a nutritional supplement, a flavoring agent, a cosmetic additive, a preparation precursor of medicines (such as antibiotics, herbicides and the like) and the like, and has great market demand.
The method for preparing L-valine by using the microbial fermentation method has the advantages of bio-based raw materials, high-efficiency conversion, mild reaction, environmental protection, economy and the like, and is widely applied to the industry. The existing fermentation device is usually a batch fermentation device, namely microorganisms are fed into a fermentation tank after primary and secondary amplification culture and are converted into glucose by using a culture medium to obtain L-valine fermentation liquor. The method has the following disadvantages: firstly, the solubility of valine is low, the solubility at 37 ℃ is 60-65 g/L, if fermentation liquor reaches a saturated state, fermentation needs to be stopped, the problems of low fermentation efficiency, high residual sugar content, high post-treatment difficulty and difficulty in guaranteeing the product quality exist, and if the fermentation time is prolonged to enable the fermentation liquor to reach a supersaturated state, valine crystals and microbial liquid are difficult to separate; secondly, during batch fermentation, the processes of primary and secondary expanding culture of microorganisms and disinfection, sterilization and cleaning of equipment need to be repeated continuously, the repeated utilization rate of bacterial liquid is low, the fermentation period is long, and the labor intensity is high; and thirdly, the L-valine fermentation liquor is subjected to sterilization, decoloration, concentration, crystallization, separation and other treatment to obtain a product, and the post-treatment steps are multiple, so that the process difficulty is high. Therefore, development of a more efficient semi-continuous fermentation plant is required.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an efficient valine semicontinuous fermentation cylinder, including intake pipe, inlet pipe, discharging pipe, agitating unit, drive arrangement, observation window, the inlet pipe sets up at the top of fermentation cylinder, and the body length of inlet pipe in the fermentation cylinder is 1/6-1/4 of fermentation cylinder length, the discharging pipe sets up at the fermentation cylinder top, and the body length of discharging pipe in the fermentation cylinder is 1/6-1/5 of fermentation cylinder length, drive arrangement sets up at the top of fermentation cylinder and connects agitating unit, agitating unit extends to the fermentation cylinder bottom from the fermentation cylinder top, is provided with the multiunit stirring rake on the agitating unit; and the tail end of the air inlet pipe is provided with an air outlet which is arranged under the stirring device.
The utility model discloses preferred technical scheme, the outside aseptic gas filter of intake-tube connection, aseptic gas filter connects air compressor.
The utility model discloses preferred technical scheme, the intake pipe is the aeration pipe that porous rubber, pottery or membrane constitute.
The utility model discloses preferred technical scheme, the fermentation cylinder is made by the material that has resistance to pressure, heat resistance and antifouling nature.
The utility model discloses preferred technical scheme, the fermentation cylinder can also further be provided with scraping device, temperature control device, water level control device, pH controlling means, pressure regulating device.
The utility model discloses a further aim at provides contain the complete sets of semicontinuous fermentation production valine of fermentation cylinder, including fermentation cylinder, air inlet unit, first feeding unit, second feeding unit, sterilization jar, separation element, washing unit and drying unit. The fermentor was a valine semi-continuous fermentor as described above.
The utility model discloses preferred technical scheme, the unit of admitting air includes air compressor and aseptic gas filter, air compressor passes through aseptic gas filter and links to each other with the intake pipe.
The utility model discloses preferred technical scheme, first feed unit includes first fermentation culture medium storage tank and first peristaltic pump, links to each other with the inlet pipe through first peristaltic pump.
The utility model discloses preferred technical scheme, second feed unit includes second fermentation medium storage tank and second peristaltic pump, links to each other with the inlet pipe through the second peristaltic pump.
The utility model discloses preferred technical scheme, the separation unit is filter press or centrifuge.
The utility model discloses preferred technical scheme, the storage tank of washing unit for being equipped with the liquid that can clean the separation membrane, liquid is the aqueous solution and the mixture of water, hydrochloric acid, sodium hydroxide.
The utility model discloses preferred technical scheme, drying unit is oven or fluidized bed.
The utility model discloses preferred technical scheme, intake pipe, the discharging pipe of fermentation cylinder access to respectively admit air unit, sterilization jar, the first feed unit of inlet pipe intercommunication and the second feed unit of fermentation cylinder, the entry of separation element and export link to each other with the export of sterilization jar, the entry of washing unit respectively, and the export of washing unit is passed through the pipeline and is linked to each other with drying unit.
The utility model discloses preferred technical scheme is equipped with the stop valve on the pipeline that intake pipe and aseptic gas filter link to each other.
The utility model discloses preferred technical scheme all is equipped with the stop valve on the pipeline that inlet pipe and first feed unit and second feed unit link to each other.
In a preferred embodiment of the present invention, the separation unit is a centrifugal separator or a separation membrane module, and the separation membrane is preferably a porous membrane having pores with an average pore diameter of 0.001um or more but less than 10 um.
The utility model discloses preferred technical scheme, the storage tank of washing unit for being equipped with the liquid that can clean the separation membrane, liquid is the aqueous solution and the mixture of water, hydrochloric acid, sodium hydroxide.
Compared with the prior art, the utility model discloses following beneficial effect has:
1. the utility model discloses can realize the semicontinuous fermentation of valine, be favorable to the result to draw and accumulate, improve the utilization ratio of fermentation cylinder, keep high concentration fermentation substrate, improve fermentation efficiency, shorten fermentation period, and keep microbial activity and further improve fermentation efficiency, the save time cost of very big degree, and then practice thrift manufacturing cost by a wide margin.
2. The semi-continuous fermentation complete equipment provided by the utility model saves the first-stage and second-stage expanding culture of microorganisms and the disinfection and cleaning process of equipment, greatly improves the utilization rate of the equipment and greatly shortens the whole process period; meanwhile, after batch fermentation is finished, all fermentation liquor needs to be subjected to multiple processes such as degerming, decoloring, evaporative crystallization and the like, the product extraction method is simple, only water washing is needed, and the process is simple and convenient; the product has good quality, greatly reduces the production cost and is suitable for industrial production.
Drawings
FIG. 1 is a schematic view of a fermentation tank according to the present invention;
FIG. 2 is a schematic view of the production system of the present invention;
the device comprises a fermentation tank 10, an air inlet pipe 11, a feeding pipe 12, a discharging pipe 13, a stirring device 14, a driving device 15, an observation window 16, a stirring paddle 17, an air inlet hole 111, an air inlet unit 20, a first feeding unit 30, a sterilization tank 40, a separation unit 50, a water washing unit 60, a drying unit 70, an air compressor 21, an aseptic gas filter 22, a first fermentation medium storage tank 31, a first peristaltic pump 32, a second feeding unit 80, a second fermentation medium storage tank 81 and a second peristaltic pump 82.
Detailed Description
The present invention will be further described with reference to the accompanying drawings.
EXAMPLE 1 valine semi-continuous fermenter
Referring to the attached figure 1, a valine semi-continuous fermentation tank 10 comprises an air inlet pipe 11, an inlet pipe 12, an outlet pipe 13, a stirring device 14, a driving device 15 and an observation window 16, wherein the inlet pipe 12 is arranged at the top of the fermentation tank 10, the pipe length of the inlet pipe 12 in the fermentation tank 10 is 1/6 of the length of the fermentation tank, the outlet pipe 13 is arranged at the top of the fermentation tank 10, the pipe length of the outlet pipe 13 in the fermentation tank 10 is 1/5 of the length of the fermentation tank, the driving device 15 is arranged at the top of the fermentation tank 10 and connected with the stirring device 14, the stirring device 14 extends from the top of the fermentation tank 10 to the bottom of the fermentation tank, and a plurality of groups of stirring paddles 17 are arranged on the stirring device 14; the tail end of the air inlet pipe 11 is provided with an air inlet hole 111, and the air inlet hole 111 is arranged right below the stirring device 14.
The air inlet pipe is an aeration pipe made of porous rubber, ceramics or a membrane. The fermenter is made of a material having pressure resistance, heat resistance and antifouling properties. The size of the hole diameter of the discharge pipe is large enough not to cause the blockage of the discharged crystal mush. The fermentation tank is further provided with a scraping device, a temperature control device, a water level control device, a pH control device and a pressure adjusting device.
EXAMPLE 2 valine semicontinuous fermentation complete sets
Referring to fig. 2, a valine semi-continuous production plant includes a fermentation tank 10, an air inlet unit 20, a feeding unit 30, a sterilizing tank 40, a separating unit 50, a water washing unit 60, and a drying unit 70. The fermentor 10 is a valine semi-continuous fermentor as described above. The air intake unit 20 includes an air compressor 21 and a sterile air filter 22, and the air compressor 21 is connected to the air intake pipe 11 through the sterile air filter 22. The first feeding unit 30 comprises a first fermentation medium storage tank 31 and a first peristaltic pump 32, and is connected with the feeding pipe 12 through the first peristaltic pump 32. The second feeding unit 80 comprises a second fermentation medium storage tank 81 and a second peristaltic pump 82, and is connected with the feeding pipe 12 through the first peristaltic pump 82. The separation unit 50 is a plate and frame filter press or a centrifuge. The water washing unit 60 is a storage tank filled with a liquid capable of cleaning the separation membrane, the liquid being an aqueous solution of water, hydrochloric acid, sodium hydroxide, or a mixture thereof. The drying unit 70 is an oven or a fluidized bed. The intake pipe 11 of the fermentation tank 10, the discharging pipe 13 lead to the air intake unit 20, the sterilization tank 40 respectively, the inlet pipe 12 of the fermentation tank 10 communicates with the first feeding unit 30 and the second feeding unit 80, the inlet and the outlet of the separation unit 50 are connected with the outlet of the sterilization tank 40 and the inlet of the water washing unit 60 respectively, and the outlet of the water washing unit 60 is connected with the drying unit 70 through a pipeline. The pipeline connecting the air inlet pipe 11 and the aseptic gas filter 22 is provided with a stop valve, and the pipeline connecting the feed pipe 12 and the first feeding unit 30 and the second feeding unit 80 is provided with a stop valve.
The specific implementation mode of the fermentation complete equipment is as follows: the fermentation culture medium and the fermentation seed liquid are inoculated into the fermentation tank 10 through the feeding pipe 12, anaerobic fermentation is carried out in a closed environment, crystal precipitation is observed from the observation window 16, the air inlet pipe is opened, and sterile air is introduced, so that the crystal gradually floats on the upper layer of the liquid level. When the pipe orifice of the discharging pipe 13 is immersed under the liquid level of the fermentation liquid containing crystals, the air inlet pipe 11 is closed, the discharging pipe 13 is opened, and the fermentation liquid containing crystals on the upper layer of the fermentation tank is discharged out of the fermentation tank 10 through the discharging pipe 13 under the action of the pressure difference between the inside and the outside of the fermentation tank and enters the sterilization tank 40. And when the liquid level of the fermentation liquid in the fermentation tank 10 is lower than the pipe opening of the discharge pipe, closing the discharge pipe, opening the feed pipe 12, performing fed-batch pumping on the fermentation culture medium, closing the feed pipe 12 after the fed-batch is completed, performing anaerobic fermentation in a closed environment, and repeating the steps.
And (3) sterilizing the fermentation liquor containing the valine crystals entering the sterilization tank 40, then sending the fermentation liquor into the separation unit 50 for filtering, sending filter residues into the water washing unit 60 for water washing, and then sending the filter residues into the drying unit 70 for drying to obtain a valine product.
The above description of the embodiments of the present invention is not intended to limit the present invention, and those skilled in the art can make various changes or modifications according to the present invention without departing from the spirit of the present invention, which should fall within the protection scope of the present invention.
Claims (11)
1. A high-efficiency valine semi-continuous fermentation tank is characterized by comprising an air inlet pipe (11), an inlet pipe (12), an outlet pipe (13), a stirring device (14), a driving device (15) and an observation window (16), wherein the inlet pipe (12) is arranged at the top of the fermentation tank (10), the pipe body length of the inlet pipe (12) in the fermentation tank (10) is 1/6-1/4 of the length of the fermentation tank (10), the outlet pipe (13) is arranged at the top of the fermentation tank (10), the pipe body length of the outlet pipe (13) in the fermentation tank (10) is 1/6-1/5 of the length of the fermentation tank (10), the driving device (15) is arranged at the top of the fermentation tank (10) and connected with the stirring device (14), and the stirring device (14) extends from the top of the fermentation tank (10) to the bottom of the fermentation tank (10), a plurality of groups of stirring paddles (17) are arranged on the stirring device (14); the tail end of the air inlet pipe (11) is provided with an air outlet hole (111), and the air outlet hole (111) is arranged right below the stirring device (14).
2. A valine semi-continuous fermenter according to claim 1, wherein the air inlet pipe (11) is connected to an external sterile air filter (22), the sterile air filter (22) is connected to an air compressor (21), and the air inlet pipe is an aeration pipe made of porous rubber, ceramic or membrane.
3. The valine semi-continuous fermenter of claim 2, wherein the valine semi-continuous fermenter is made of a material having pressure resistance, heat resistance and antifouling property.
4. The valine semi-continuous fermenter according to claim 3, further comprising a scraper and a temperature control device and a water level control device and a pH control device and a pressure adjusting device.
5. A plant, characterized by comprising the valine semicontinuous fermenter according to any one of claims 1 to 4, a gas inlet unit (20), a first feed unit (30), a second feed unit (80), a sterilization tank (40), a separation unit (50), a water washing unit (60), and a drying unit (70), wherein a gas inlet pipe (11) of the valine semicontinuous fermenter is connected to the gas inlet unit (20), a feed pipe (12) is connected to the first feed unit (30) and the second feed unit (80), a discharge pipe (13) is connected to the sterilization tank (40), an outlet of the sterilization tank (40) is connected to an inlet of the separation unit (50), an outlet of the separation unit (50) is connected to an inlet of the water washing unit (60), and an outlet of the water washing unit (60) is connected to the drying unit (70).
6. The plant according to claim 5, characterized in that the air intake unit (20) comprises an air compressor (21) and a sterile gas filter (22) connected to each other, the air intake pipe (11) being connected to the gas outlet of the sterile gas filter (22).
7. The plant according to claim 6, characterized in that said first feeding unit (30) comprises a first fermentation medium tank (31) connected to the feeding pipe (12), the pipe of the feeding pipe (12) being provided with a first peristaltic pump (32); the second feeding unit (80) comprises a second fermentation medium storage tank (81) connected with the feeding pipe (12), and a second peristaltic pump (82) is arranged on the pipeline of the feeding pipe (12).
8. The plant according to claim 7, characterized in that the separation unit (50) is a plate and frame filter press or a centrifuge, the water washing unit (60) is a tank containing a liquid capable of cleaning the separation membrane, and the drying unit (70) is an oven or a fluidized bed.
9. The plant according to claim 8, characterized in that the inlet pipe (11) and the outlet pipe (13) are respectively connected to the inlet unit (20) and the sterilization tank (40), the inlet pipe (12) is connected to the first inlet unit (30) and the second inlet unit (80), the inlet and the outlet of the separation unit (50) are respectively connected to the outlet of the sterilization tank (40) and the inlet of the water washing unit (60), and the outlet of the water washing unit (60) is connected to the drying unit (70) through a pipeline.
10. The plant according to claim 9, characterized in that the pipeline connecting the gas inlet pipe (11) with the sterile gas filter (22) is provided with a stop valve, and the pipeline connecting the gas inlet pipe (12) with the first fermentation medium storage tank (31) and the second fermentation medium storage tank (81) is provided with a stop valve.
11. The kit of claim 10, wherein the separation unit is a centrifugal separation device, or a separation membrane module, the separation membrane is a porous membrane having pores with an average pore size of 0.001um or more but less than 10um, and the water washing unit is a reservoir containing a liquid capable of cleaning the separation membrane.
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| CN202122161403.2U CN217324059U (en) | 2021-09-08 | 2021-09-08 | Efficient valine semicontinuous fermentation cylinder and complete sets |
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| CN202122161403.2U CN217324059U (en) | 2021-09-08 | 2021-09-08 | Efficient valine semicontinuous fermentation cylinder and complete sets |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015559A (en) * | 2021-09-08 | 2022-02-08 | 安徽华恒生物科技股份有限公司 | High-efficiency valine semi-continuous fermentation method and complete equipment thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015559A (en) * | 2021-09-08 | 2022-02-08 | 安徽华恒生物科技股份有限公司 | High-efficiency valine semi-continuous fermentation method and complete equipment thereof |
| CN114015559B (en) * | 2021-09-08 | 2023-06-23 | 安徽华恒生物科技股份有限公司 | Efficient valine semi-continuous fermentation method and complete equipment thereof |
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