CN217077598U - Spirulina is collected and inoculation device in laboratory - Google Patents

Abstract

The utility model discloses a laboratory is with spirulina collection and inoculation device, include: the algae liquid storage tank is of a hollow structure, and the top end and the bottom end of the algae liquid storage tank are both open; the filtrate storage tank is of a hollow structure, the bottom end of the filtrate storage tank is open, the top of the filtrate storage tank is fixedly connected with a bearing layer, a plurality of filtering holes are formed in the bearing layer, and the filtering holes are communicated with the inner cavity of the filtrate storage tank; the bolting-silk is placed on the bearing layer, and the shape of the bolting-silk is matched with that of the bearing layer; the vacuum generator comprises a vacuum generating cylinder and a piston rod assembly; the piston rod assembly is connected with the inner wall of the vacuum generating cylinder in a sliding way; wherein, the top and the bottom of the filtrate holding tank are respectively detachably connected with the algae liquid holding tank and the vacuum generating cylinder. The utility model discloses when can realizing that the spirulina body is gathered fast, still can realize separating the whole vaccinations that are used for once more of the spirulina body.

Description

Spirulina is collected and inoculation device in laboratory

Technical Field

The utility model relates to a little algae cultivation technical field especially relates to a laboratory is with spirulina collection and inoculation device.

Background

Microalgae is a biomass resource with a great application prospect, and the excessive recovery cost is a technical bottleneck limiting the development of the microalgae industry. At present, the main technical methods for harvesting microalgae comprise a separation method, a filtration method, a sedimentation method, a flotation method, a flocculation method and the like. The centrifugal separation method has the advantages of simple operation, high efficiency, no additive and wide application range, but has the problems of high energy consumption, high equipment operation and maintenance cost investment and the like when the technology is used on a large scale. Secondly, the filtration method is simple and convenient to operate, and no chemical reagent is needed to be added, so that toxic and harmful substances are not introduced, but the filtration membrane is greatly influenced by the outside and the culture solution environment, and the problem of membrane pollution is easily caused. The sedimentation method tends to collect microalgae with higher cell density, the technology is greatly influenced by environmental factors, and has the problems of low efficiency, long time consumption and the like, and the sedimentation method is generally combined with flocculation, centrifugation and other technologies in actual production to improve the efficiency. The air floatation method has high efficiency, but agents such as foaming agents and the like can be introduced, which can have adverse effect on the growth of the microalgae and cause pollution to the downstream process. The flocculation method is divided into chemical flocculation, physical flocculation and biological flocculation, wherein the chemical flocculation has high efficiency, but chemical flocculants are introduced to cause pollution; the physical flocculation can avoid the pollution problem to a certain extent, and part of processes are complex and the cost is high; the biological flocculation is environment-friendly, pollution-free, high in safety, simple to operate, low in cost, poor in applicability and low in efficiency. Therefore, the prior art has the advantages and disadvantages respectively, and different harvesting methods are selected according to the characteristics of specific algae.

In the process of culturing the microalgae in the laboratory, because the culture volume is small, the centrifugal method and the filtering method can basically meet the experimental requirements, however, aiming at the filamentous microalgae such as the spirulina, the centrifugal method can not completely precipitate the algae body in the initial stage and the middle stage of the culture, and most of the algae can exist in a suspension state after the centrifugation, so the centrifugal method is not completely applicable; because of the filamentous structure of spirulina body, the filtration method is a very simple and convenient harvesting method, the method of 0.45 mu m filter membrane suction filtration is commonly used in laboratories to harvest spirulina for calculating biomass and the like, the spirulina body harvested by the method is tightly attached to the filter membrane, the re-inoculation and re-culture of the spirulina are difficult to realize, 300-mesh or 500-mesh bolting silk is industrially used for separating the spirulina in the water body, the method is suitable for large-scale culture, the harvesting cost can be greatly reduced, but for small-scale culture in laboratories, such as 200mL of spirulina liquid, the spirulina can be effectively intercepted at the initial stage of filtration, and along with the increase of the filtered spirulina liquid, the spirulina blocks the aperture of the bolting silk, so that the filtration rate becomes slow or even stops; especially for the experiment of reseeding, several milliliters of algae liquid can not realize the collection of spirulina through bolting silk by the action of gravity due to the action of surface tension of water, the algae on the filter membrane is forcibly scraped for reseeding culture, the separated algae can be damaged, the spirulina biomass can be scraped to only occupy a small part of the separated algae, and the growth rate of the reseeding culture spirulina is severely restricted. Therefore, a spirulina collecting and inoculating device for a laboratory is provided.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a spirulina collects and inoculation device in laboratory aims at solving or improves at least one among the above-mentioned technical problem.

In order to achieve the above object, the utility model provides a following scheme: the utility model provides a device is collected and inoculates with spirulina in laboratory, include:

the algae liquid storage tank is of a hollow structure, and the top end and the bottom end of the algae liquid storage tank are both opened;

the filtrate storage tank is of a hollow structure, the bottom end of the filtrate storage tank is open, a bearing layer is fixedly connected to the top of the filtrate storage tank, a plurality of filtering holes are formed in the bearing layer, and the filtering holes are communicated with the inner cavity of the filtrate storage tank;

the bolting silk is placed on the bearing layer, and the shape of the bolting silk is matched with that of the bearing layer;

the vacuum generator comprises a vacuum generating cylinder and a piston rod assembly; the piston rod assembly is connected with the inner wall of the vacuum generating cylinder in a sliding way;

wherein, the top and the bottom of the filtrate holding tank are detachably connected with the algae liquid holding tank and the vacuum generating cylinder respectively.

Preferably, one end of the piston rod assembly extends out of the vacuum generation cylinder, the top of the filtrate storage tank is in threaded connection with the bottom of the algae liquid storage tank, and the bottom of the filtrate storage tank is in threaded connection with one end, far away from the piston rod assembly, of the vacuum generation cylinder.

Preferably, the bottom of the algae liquid holding tank and the bottom of the filtrate holding tank are both provided with internal thread interfaces, the top of the filtrate holding tank and the top of the vacuum generating barrel are both provided with external thread interfaces, and the internal thread interfaces are matched with the external thread interfaces.

Preferably, the piston rod assembly comprises a piston and a pull rod, the pull rod is fixedly connected with the piston, the piston is in sliding connection with the inner side wall of the vacuum generation cylinder, and one end of the pull rod extends out of the vacuum generation cylinder.

Preferably, the connection parts of the algae liquid storage tank, the vacuum generating cylinder and the filtrate storage tank are provided with sealing rings.

Preferably, the filtering holes are circumferentially arranged at equal intervals along the center of the supporting layer, and the aperture of each filtering hole is not smaller than 3 mm.

Preferably, the piston is a rubber piston.

The utility model discloses a following technological effect:

the utility model applies external pressure to the algae liquid storage tank and the filtrate storage tank by pulling the piston rod assembly, so that the spirulina culture solution passes through the bolting silk under the pressure driving action, the culture solution is filtered out, and the algae body is intercepted; for a large amount of spirulina culture solution, even if the initial algae block the pore size of the bolting silk, the continuous filtration of the subsequent algae solution can be still realized under the action of pressure, thereby solving the problems that the trace algae solution can not pass through the bolting silk and the problem that the initial algae block the pore size of the bolting silk so as to slow down the later filtration speed and even stop the filtration;

the utility model pours the spirulina culture solution into the spirulina solution storage tank, pulls the piston rod assembly downwards, makes the spirulina solution storage tank and the filtrate storage tank bear pressure in a vacuum pressure generating mode, accelerates the separation of the culture solution and the spirulina body in the spirulina culture solution, and realizes the rapid collection of the spirulina body in the small-scale spirulina culture solution in the laboratory; meanwhile, the algae can be effectively prevented from being crushed due to forced scraping, and the purpose of using all separated algae for secondary inoculation is realized; if the filtered spirulina liquid is a spirulina culture liquid, when salt on the surface of spirulina body of the spirulina needs to be washed for analyzing physiological and biochemical indexes, proper amount of distilled water is poured into the filtered spirulina liquid storage tank, the piston rod assembly is pulled, and the washing operation of the salt on the surface of the spirulina liquid can be realized by repeating the operation for many times.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural view of a spirulina collecting and inoculating device for a laboratory of the present invention;

FIG. 2 is a schematic view of a screen cloth according to the present invention;

FIG. 3 is an assembly diagram of the bolting silk, the algae liquid storage tank and the filtrate storage tank of the utility model;

wherein, 1, internal thread; 2. an external thread; 3. a support layer; 4. a seal ring; 5. a piston; 6. a pull rod; 7. screening silk; 8. a algae liquid storage tank; 9. a filtrate holding tank; 10. a vacuum generating cylinder; 11. and (4) filtering holes.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

Referring to fig. 1-3, the utility model provides a spirulina collects and inoculation device is used in laboratory, include:

the algae liquid storage tank 8 is of a hollow structure, and the top end and the bottom end of the algae liquid storage tank 8 are both open;

the filtrate storage tank 9 is of a hollow structure, the bottom end of the filtrate storage tank 9 is open, the top of the inner side wall of the filtrate storage tank 9 is fixedly connected with a supporting layer 3, a plurality of filtering holes 11 are formed in the supporting layer 3, and the filtering holes 11 are communicated with the inner cavity of the filtrate storage tank 9; the supporting layer 3 is of a circular plate structure, is made of the same material as the filtrate storage tank 9 and is used for bearing the bolting silk 7;

the bolting-silk 7 is placed on the bearing layer 3, and the shape of the bolting-silk is matched with that of the bearing layer 3; the bolting silk 7 is flatly laid on the bearing layer 3, and the bolting silk 7 is fixed by assembling the filtrate storage tank 9 and the algae liquid storage tank 8; the mesh number of the bolting silk 7 can be set according to the specific use environment, and is not specifically limited in this embodiment;

the vacuum generator comprises a vacuum generating cylinder 10 and a piston rod assembly; the piston rod assembly is connected with the inner wall of the vacuum generating cylinder 10 in a sliding way; according to the arrangement, external pressure is applied to the algae liquid storage tank 8 and the filtrate storage tank 9 by pulling the piston rod assembly, so that the spirulina culture solution passes through the bolting silk under the pressure driving effect, the culture solution is filtered out, and algae are intercepted; for a large amount of spirulina culture solution, even if the initial algae block the pore size of the bolting silk, the continuous filtration of the subsequent algae solution can be still realized under the action of pressure, thereby solving the problems that the micro algae solution can not pass through the bolting silk 7 and the problem that the initial algae block the pore size of the bolting silk 7 to slow down the later filtration speed and even stop the filtration; the utility model can realize the rapid collection of the algae in the laboratory small-scale spirulina culture solution, and can effectively avoid the algae crushing caused by forced scraping, thereby realizing the purpose of using the separated algae for secondary inoculation;

wherein, the top and the bottom of the filtrate holding tank 9 are respectively detachably connected with the algae liquid holding tank 8 and the vacuum generating cylinder 10;

according to the arrangement, when in use, the bolting silk 7 is firstly tiled on the bearing layer 3, then the filtrate storage tank 9, the algae liquid storage tank 8 and the vacuum generating cylinder 10 are assembled, and the bolting silk 7 is fixed between the algae liquid storage tank 8 and the bearing layer 3, and then the piston rod assembly is pushed to one end close to the filtrate storage tank 9; pouring a proper amount of spirulina culture solution to be filtered into the inner cavity of the spirulina liquid storage tank 8 through the top opening of the spirulina liquid storage tank 8, pulling the piston rod assembly downwards, enabling the spirulina liquid to flow downwards by the generated vacuum pressure and flow into the filtrate storage tank 9 and the vacuum generation barrel 10 through the filter holes 11 on the bolting silk 7 and the bearing layer 3, and leaving the filtered spirulina liquid in the spirulina liquid storage tank 8;

if the density of algae cells in the algae liquid is low, pouring a proper amount of spirulina culture solution to be filtered into the inner cavity of the algae liquid storage tank 8 again, repeating the process, when the piston rod assembly is pulled to the bottom of the vacuum generating cylinder 10, detaching the vacuum generating cylinder 10 from the filtrate storage tank 9, pouring out the filtrate, resetting the piston rod assembly, assembling the vacuum generating cylinder 10 and the filtrate storage tank 9, repeating the operation again, and continuously filtering; after the required filtration is finished, separating the filtrate storage tank 9 from the vacuum generating cylinder 10, connecting the top of the algae liquid storage tank 8 with a conical flask, inverting a connecting body of the algae liquid storage tank 8 and the filtrate storage tank 9, and pouring fresh culture solution into an opening at one end of the filtrate storage tank 9 far away from the algae liquid storage tank 8, so that the fresh culture solution rushes the spirulina on the bolting silk 7 into the conical flask along the filtrate storage tank 9 for inoculation and culture again; meanwhile, the piston rod assembly can be pushed to the bottom of the vacuum generating cylinder 10, after the top of the vacuum generating cylinder is assembled with the filtrate storage tank 9, the piston rod assembly is pushed to accelerate the fresh culture solution to flush the spirulina attached to the bolting silk 7, and the efficiency of inoculation culture is improved.

According to a further optimization scheme, one end of the piston rod assembly extends out of the vacuum generation cylinder 10, the top of the filtrate storage tank 9 is in threaded connection with the bottom of the algae liquid storage tank 8, and the bottom of the filtrate storage tank 9 is in threaded connection with one end, far away from the piston rod assembly, of the vacuum generation cylinder 10;

the bottom of the algae liquid storage tank 8 and the bottom of the filtrate storage tank 9 are both provided with an internal thread 1 interface, the top of the filtrate storage tank 9 and the top of the vacuum generation barrel 10 are both provided with an external thread 2 interface, and the internal thread 1 interface is matched with the external thread 2 interface; the threaded connection of the internal thread 1 and the external thread 2 can realize the quick assembly and disassembly of the algae liquid holding tank 8, the filtrate holding tank 9 and the vacuum generating cylinder 10.

According to a further optimized scheme, the piston rod assembly comprises a piston 5 and a pull rod 6, the pull rod 6 is fixedly connected with the piston 5, the piston 5 is connected with the inner side wall of the vacuum generating cylinder 10 in a sliding mode, and one end of the pull rod 6 extends out of the vacuum generating cylinder 10; the piston 5 is a rubber piston; experimenters pull the pull rod 6 to drive the piston 5 to slide up and down along the inner wall of the vacuum generating cylinder 10, and then pressurization operation and reset operation are realized.

Further optimizing scheme, the sealing washer 4 is installed to the junction of algae liquid holding tank 8, vacuum generation section of thick bamboo 10 and filtrating holding tank 9 for it is sealed, isolated air.

Further optimizing scheme, filter 11 along the central department circumference equidistant arrangement of layer 3 of supporting to filter 11 aperture is not less than 3mm, can be convenient for when being convenient for process the filtrating and oozing.

In a further optimized scheme, the algae liquid storage tank 8, the filtrate storage tank 9 and the vacuum generating cylinder 10 are all made of transparent materials; the material of algae liquid holding tank 8, filtrate holding tank 9 and vacuum generation section of thick bamboo 10 can be set for according to specific service environment, is transparent ya keli in this embodiment, adopts thickness to make for 5 mm's transparent ya keli board, is convenient for observe the algae liquid separation condition in algae liquid holding tank 8, filtrate holding tank 9 and the vacuum generation section of thick bamboo 10.

Further optimizing the scheme, the inner diameters of the algae liquid storing groove 8 and the filtrate storing groove 9 are both 40mm, the heights are both 50mm, the inner diameter of the vacuum generating cylinder 10 is 40mm, the height is 120mm, and the height of the pull rod is 130 mm.

The working principle is as follows:

when the utility model is used, the spirulina collecting and inoculating device for the laboratory firstly lays the bolting silk 7 on the bearing layer 3, then assembles the filtrate storage tank 9, the algae liquid storage tank 8 and the vacuum generating cylinder 10 and fixes the bolting silk 7 between the algae liquid storage tank 8 and the bearing layer 3, and then pushes the pull rod 6 to make the piston 5 push to one end close to the filtrate storage tank 9; pouring a proper amount of spirulina culture solution to be filtered into the inner cavity of the spirulina liquid holding tank 8 through the top opening of the spirulina liquid holding tank 8, pulling the pull rod 6 downwards, leading the spirulina liquid to flow downwards by the generated vacuum pressure and flow into the filtrate holding tank 9 and the vacuum generating cylinder 10 through the bolting silk 7 and the filtering holes 11 on the supporting layer 3, and leaving the filtered spirulina liquid in the spirulina liquid holding tank 8;

if the density of algae cells in the algae liquid is low, pouring a proper amount of spirulina culture solution to be filtered into the inner cavity of the algae liquid storage tank 8 again, repeating the process, when the piston 5 is pulled to the bottom of the vacuum generating cylinder 10, detaching the vacuum generating cylinder 10 from the filtrate storage tank 9, pouring out the filtrate, resetting the piston rod assembly, assembling the vacuum generating cylinder 10 and the filtrate storage tank 9, repeating the operation again, and continuously filtering; after the required filtration is finished, separating the filtrate holding tank 9 from the vacuum generation cylinder 10, connecting the top of the algae liquid holding tank 8 with a conical flask (not shown in the figure), inverting the connecting body of the algae liquid holding tank 8 and the filtrate holding tank 9, and pouring fresh culture solution through an opening at one end of the filtrate holding tank 9 far away from the algae liquid holding tank 8, so that the fresh culture solution flushes the spirulina on the bolting silk 7 into the conical flask (not shown in the figure) along the filtrate holding tank 9 for inoculation and culture again; meanwhile, the piston 5 can be pushed to the bottom of the vacuum generating cylinder 10, after the top of the vacuum generating cylinder is assembled with the filtrate storage tank 9, the fresh culture solution is accelerated to wash the spirulina attached to the bolting silk 7 by pushing the pull rod 6, and the efficiency of inoculation culture is improved.

If the filtered spirulina liquid is a spirulina culture liquid, when the salt on the surface of the spirulina body needs to be washed for physiological and biochemical index analysis, a proper amount of distilled water is poured into the filtered spirulina liquid storage tank 8, the pull rod 6 is pulled, and the washing operation of the salt on the surface of the spirulina liquid can be realized by repeating the operation for many times.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

The above-mentioned embodiments are only intended to describe the preferred embodiments of the present invention, but not to limit the scope of the present invention, and those skilled in the art should also be able to make various modifications and improvements to the technical solution of the present invention without departing from the spirit of the present invention, and all such modifications and improvements are intended to fall within the scope of the present invention as defined in the appended claims.

Claims (7)

1. The utility model provides a device is collected and inoculation with spirulina in laboratory which characterized in that includes:

the algae liquid storage tank (8) is of a hollow structure, and the top end and the bottom end of the algae liquid storage tank (8) are both opened;

the filter liquor storage tank (9) is of a hollow structure, the bottom end of the filter liquor storage tank (9) is open, a bearing layer (3) is fixedly connected to the top of the filter liquor storage tank (9), a plurality of filter holes (11) are formed in the bearing layer (3), and the filter holes (11) are communicated with the inner cavity of the filter liquor storage tank (9);

the bolting silk (7) is placed on the bearing layer (3) and is matched with the bearing layer (3) in shape;

the vacuum generator comprises a vacuum generating cylinder (10) and a piston rod assembly; the piston rod assembly is connected with the inner wall of the vacuum generating cylinder (10) in a sliding way;

wherein the top and the bottom of the filtrate storing groove (9) are respectively detachably connected with the algae liquid storing groove (8) and the vacuum generating barrel (10).

2. The laboratory spirulina collecting and inoculating device of claim 1, wherein: one end of the piston rod assembly extends out of the vacuum generation cylinder (10), the top of the filtrate storage tank (9) is in threaded connection with the bottom of the algae liquid storage tank (8), and the bottom of the filtrate storage tank (9) is in threaded connection with one end, far away from the piston rod assembly, of the vacuum generation cylinder (10).

3. The laboratory spirulina collecting and inoculating device of claim 2, wherein: the bottom of the algae liquid holding tank (8) and the bottom of the filtrate holding tank (9) are both provided with internal threads (1) and external threads (2) and the internal threads (1) and the external threads (2) are matched with each other, and the top of the filtrate holding tank (9) and the top of the vacuum generating barrel (10) are both provided with external threads (2) and the internal threads (1) and the external threads (2) are matched with each other.

4. The laboratory spirulina collecting and inoculating device of claim 1, wherein: the piston rod assembly comprises a piston (5) and a pull rod (6), the pull rod (6) is fixedly connected with the piston (5), the piston (5) is connected with the inner side wall of the vacuum generating cylinder (10) in a sliding mode, and one end of the pull rod (6) extends out of the vacuum generating cylinder (10).

5. The laboratory spirulina collecting and inoculating device of claim 1, wherein: and a sealing ring (4) is arranged at the joint of the algae liquid storage tank (8), the vacuum generating cylinder (10) and the filtrate storage tank (9).

6. The laboratory spirulina collecting and inoculating device of claim 1, wherein: the filtering holes (11) are circumferentially arranged at equal intervals along the center of the supporting layer (3), and the aperture of each filtering hole (11) is not smaller than 3 mm.

7. The laboratory spirulina collecting and inoculating device of claim 4, wherein: the piston (5) is a rubber piston.

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