CN108823072B - Culture-harvesting integrated system and method for microalgae cells - Google Patents

Culture-harvesting integrated system and method for microalgae cells Download PDF

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CN108823072B
CN108823072B CN201811095144.4A CN201811095144A CN108823072B CN 108823072 B CN108823072 B CN 108823072B CN 201811095144 A CN201811095144 A CN 201811095144A CN 108823072 B CN108823072 B CN 108823072B
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harvesting
bed
culture solution
culture
filter residue
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CN108823072A (en
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吕雪峰
段仰凯
张凯
吴怀之
刘祥
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Qingdao Zhongke Lanzhi Biotechnology Development Co ltd
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Qingdao Zhongke Lanzhi Biotechnology Development Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Abstract

The application relates to a culture-harvesting integrated system of microalgae cells, belonging to the technical field of culture and harvesting of microalgae. The culture system comprises: the device comprises a photoreactor, an air inlet pipe, a stirrer, an air outlet and a light source; one end of the air inlet pipe is inserted into the culture solution of the photoreactor; the stirrer is arranged in the photoreactor; the exhaust port is arranged at the upper part of the liquid level of the culture solution; the light source is arranged inside and/or outside the photoreactor; the harvesting system consists of a filter residue bed and a harvesting bed, wherein the filter residue bed is arranged above the harvesting bed, and the photoreactor in the culturing system is connected with the filter residue bed through a pipeline. According to the application, after microalgae cells are cultured by a photosynthesis culture process, the microalgae cells are rapidly collected by the collection bed with the inclination angle changing from 0 degrees to 90 degrees continuously or in a gradient manner, so that the microalgae cells are thoroughly separated from culture solution, the problem of unsmooth discharge of the microalgae cells is effectively avoided, and the collection efficiency of the microalgae cells is greatly improved.

Description

Culture-harvesting integrated system and method for microalgae cells
Technical Field
The application relates to the technical field of microalgae culture and harvesting, in particular to a microalgae cell culture-harvesting integrated system and method.
Background
Microalgae are autotrophic plants widely distributed in natural environments, and not only can synthesize high-added-value compounds such as grease, sugar, protein, carotene and the like through photosynthesis, but also are considered as one of the most important grain resources in the future of possible human beings by international grain and agriculture organizations due to high photosynthetic efficiency, fast growth, high per mu yield and the like. Because of the tiny volume of the algae cells, the harvesting is difficult and the harvesting cost is high, and the method becomes one of the difficult problems of restricting the development and large-scale popularization of the microalgae industry. For this reason, a variety of algae cultivation apparatuses have been developed, such as patent CN206872835U discloses an algae cultivation apparatus comprising: the culture container is placed in a magnetic induction area of the magnetic stirrer, and the stirrer is placed in the culture container; clamping ends of the heating rod and the temperature sensor are clamped on the bracket, and a heating end of the heating rod and an induction end of the temperature sensor extend into the culture container; the bracket is fixed on the base of the magnetic stirrer; each LED and each flexible circuit board are fixed on the outer side wall and the bottom of the culture container after being assembled, and each LED is positioned between each flexible circuit board and the culture container; compared with the prior art, the temperature control of the algae culture solution is accurate and efficient, the illumination is uniform and sufficient, but the culture device has a complex structure and high manufacturing cost.
The harvesting of microalgae is also an important link for obtaining metabolites, and the common microalgae harvesting method mainly comprises flocculation sedimentation, filtration or centrifugal separation, and the like, wherein flocculant is required to be added in the flocculation sedimentation, so that the recycling of the cultivation wastewater is difficult, the pollution and the waste of water resources are caused, and the industrialized microalgae harvesting is difficult to use. The centrifugal separation cost is high, the kinetic energy of high-speed rotation is required, the harvesting energy consumption is extremely high, and the method is mainly used for laboratory small-scale harvesting analysis. Therefore, the filtration is a main microalgae harvesting method, and the main filtration harvesting method at home and abroad is finished through a manually controlled screen device, for example, the spirulina is manufactured into unpowered screening devices such as a flat screen, a pocket screen or an inclined screen by adopting a 300-380 mesh soft screen, and harvesting is realized through a single-stage inclined filter bed or a multi-stage inclined filter bed. This kind of system of gathering, algae liquid like waterfall flows through from the inclined plane filter bed generally, and moisture permeates the filter screen and algae mud adheres to on the guipure, need utilize the washing of rivers, and the device of this kind of structure not only gathers the system area very big, and construction cost is also higher, and it is piled up to need incessant rivers washing to prevent algae mud moreover, not only influences the gathering, and unmanned management degree of difficulty is very big moreover. In addition, the algae mud collected by the flat screen or inclined screen platform needs to flow into a collection pool, and needs to be transferred to an algae mud dehydrator or other drying equipment or containers for further processing by using additional power such as a piston pump, so that the process flow is long and the collection efficiency is low.
In order to solve the practical problems of low harvesting efficiency and large occupied area of a flat screen or inclined screen device, chinese patent application CN 102696340A proposes an algae liquid harvesting and dewatering device with inclined rotary drum filters connected in series, and the core is to drive a multistage rotary drum to rotate and separate algae liquid and algae mud by using a driving device of the rotary drum, so that pumped algae liquid is rotationally moved in the rotary drum filter. Compared with the existing unpowered flat screen, the device has the advantages that a heavy harvesting film platform needs to be driven to rotate by using a high-power driving device, the power consumption for harvesting is greatly increased due to the fact that the film harvesting device is large and clumsy, and compared with the existing unpowered multi-stage flat screen, the device has the technical advantages that the technical advantages are not obvious, but the problem that harvesting is unsmooth due to accumulation of algae mud on the surface of a roller filtering film is solved.
In summary, the existing culture and harvesting methods and systems of microalgae cells still have a series of problems of complex culture process, high cost, large occupied area of harvesting devices, low harvesting efficiency and the like, so that a new culture and harvesting system of microalgae cells is necessary to be studied.
Disclosure of Invention
Aiming at the problems in the prior art, the application aims to provide a culture-harvesting integrated system and method for microalgae cells. According to the application, microalgae cells are cultivated through an integrally designed photosynthetic cultivation process, and then the microalgae cells are rapidly harvested through the harvesting bed with the inclination angle continuously changing from 0 degrees to 90 degrees or changing in a gradient manner, so that the microalgae cells are thoroughly separated from the culture solution and sufficiently cleaned, the problems of unsmooth discharge and accumulation of the microalgae cells in the harvesting bed are effectively avoided, the harvesting efficiency of the microalgae cells is greatly improved, and the efficient cultivation and harvesting of the microalgae cells are realized.
The application aims at providing a culture-harvesting integrated system of microalgae cells.
The second object of the present application is to provide a method for culturing and harvesting microalgae cells.
The application also aims to provide a method for culturing and harvesting the spirulina for synthesizing the glyceroglycosides.
In order to achieve the above purpose, the application specifically discloses the following technical scheme:
firstly, the application provides a culture-harvesting integrated system of microalgae cells, which comprises a culture system and a harvesting system; the culture system is used for culturing microalgae cells, and the harvesting system is used for rapidly separating the microalgae cells from the culture solution.
Specifically, the culture system includes: the device comprises a photoreactor, an air inlet pipe, a stirrer, an air outlet and a light source.
The photoreactor is used for bearing microalgae cells and culture solution, one end of the air inlet pipe is inserted into the culture solution, and the other end of the air inlet pipe is connected with an air source containing carbon source gas, so that carbon elements are provided for the photosynthetic growth of the microalgae cells; preferably, the carbon source-containing gas is CO-containing 2 Is a gas of (a) a gas of (b).
The stirrer is arranged in the photoreactor, so that microalgae cells in the photoreactor are more uniform in the aspects of receiving illumination, nutrition supply and carbon source gas absorption.
The exhaust port is arranged at the upper part of the liquid level of the culture solution, so that the gas in the photoreactor can smoothly enter and exit, and the timely discharge of oxygen generated by the photosynthesis of microalgae cells can be ensured.
The recovery system includes: the filter residue bed and the harvesting bed are arranged above the harvesting bed, the central axes of the filter residue bed and the harvesting bed are coincident, and microalgae cells enter the filter residue bed after completing culture in a photoreactor of a culture system.
The filter residue bed is of a truncated cone-shaped structure with the diameter of the lower end face larger than that of the upper end face, and the side face of the filter residue bed forms a bed surface of a filtering structure so as to filter large-particle impurities in the mixture of the collected microalgae cells and the culture solution.
The harvesting bed is of a variable-camber horn funnel-shaped structure, the side wall of the harvesting bed forms a bed surface of a membrane hole filtering structure, namely the harvesting bed surface, and the inclination angle alpha of the bed surface relative to the horizontal plane is continuously changed from 0 degree to 90 degrees or is changed in a gradient manner. The microalgae cells and the culture solution which are filtered by the filter residue bed to remove impurities enter the harvesting bed surface, most of the culture solution is filtered out from the membrane holes of the harvesting bed surface in the flowing process, and the microalgae cells are trapped on the bed surface, so that the quick separation of the microalgae cells and the culture solution is realized, the microalgae cells of most of the culture solution are separated to form algae mud, and the algae mud is discharged from the algae mud output port at the bottom of the harvesting bed after being collected.
Preferably, the gradient change of the inclination angle alpha of the recovery bed surface relative to the horizontal plane is divided into 4 gradients: the gradient change is characterized by a slow initial gradient of the bed surface, so that the bed surface separation area is large, the rapid separation of a large amount of culture solution is facilitated, the gradient of the collected bed surface is rapidly increased along with the rapid separation of the culture solution, the bed surface separation area is rapidly contracted, the flow of microalgae cells and the rapid discharge of the collected bed are facilitated, and the microalgae cells are prevented from accumulating.
The light source is arranged inside (i.e. internally illuminated) and/or outside the photoreactor; as long as can provide sufficient illumination for the photosynthesis of algae cells, when the light source is arranged outside the photoreactor, the illumination can be provided for the algae cells by directly irradiating the culture solution, and the illumination can also be provided for the algae cells by indirectly irradiating the culture solution through transparent materials.
The form of the photoreactor is not limited, and the photoreactor can be a closed tubular reactor, a flat plate type reactor or a cylindrical photoreactor; or an open runway pool; the microalgae can grow rapidly as long as the light irradiation from the liquid surface can be ensured.
The type of the microalgae cells is not limited, and may be green algae, diatoms, blue algae, etc., cyanobacteria, or other genetically modified photosynthetic microorganisms, etc., as long as they can satisfy the metabolic products required for production, and spirulina is preferable.
Preferably, the culture solution is not limited in type, and can be a seawater culture solution, such as a common f/2 culture solution, or a fresh water culture solution, such as a common BG; the culture medium may be an acidic culture medium or an alkaline culture medium, such as Zarok alkaline culture medium or MC green algae culture medium, which is modified so long as it satisfies the nutrient supply and other physicochemical conditions required for growth of microalgae cells.
Preferably, the culture solution comprises nutrients such as water, nitrogen, phosphorus, calcium, magnesium, iron, trace metal nutrient salts and the like.
The material and shape form of the air inlet pipe are not limited, and inorganic mineral substances such as cement, ceramics, quartz sand and the like can be used; or plastic materials such as polyethylene, polypropylene and rubber; or may be metal, such as copper, stainless steel, etc., and the shape is not limited, and may be pipe, square, round, etc.; the shape and the number of the vent holes are not limited, and the vent holes are single-hole and porous; as long as carbon dioxide gas can be ensured to be introduced into the culture solution.
The material and shape of the stirrer are not limited, and the stirrer can be spiral, similar to a paddle, and the like, so long as uniform mixing of gas, solid and liquid can be realized.
Preferably, the air inlet pipe and the stirrer are integrally designed: the stirrer is provided with the air inlet pipeline and the air outlet hole, and carbon source-containing gas enters the culture solution from the air outlet hole after passing through the air inlet pipeline, so that the stirring can be realized while ventilation is realized, and the entering gas is more uniformly mixed into the culture solution.
The form of the light source is not limited, and the light source can be natural sunlight or artificial light source, so long as the light source can provide light wavelength which can meet the light synthesis requirement of microalgae cells.
Preferably, the artificial light source includes an LED, a fluorescent lamp, a mercury lamp, or the like.
Further, the harvesting system further comprises a liquid inlet pipe, the liquid inlet pipe is connected with the culture system, the liquid inlet pipe is annularly fixed at the upper end of the bed surface of the filter residue bed, a plurality of liquid distribution holes with jet flow directions facing the filter residue bed are formed in the liquid inlet pipe, and the arrangement of the liquid distribution holes realizes the balanced distribution of culture liquid containing microalgae cells on the bed surface of the filter residue bed.
Preferably, the filter residue bed surface is a membrane hole filtering structure, and the membrane hole filtering structure is made of a hard or soft filtering material with membrane holes of 10-100 meshes.
Preferably, the bed surface of the recovery bed is a membrane hole filtering structure, and the membrane hole filtering structure is made of a hard or soft filtering material with 100-800 meshes of membrane holes.
Further, the harvesting system also comprises a filter residue collecting tank, a filter residue cleaning rod, a rotating wheel, a filter residue discharging port, a slag discharging channel and a rotating shaft; the filter residue collecting tank is arranged on the periphery of the lower end face of the filter residue bed, is tightly connected with the filter residue bed, the filter residue discharge port is arranged in the filter residue collecting tank, the slag discharge channel is connected with the filter residue discharge port, and the filter residue cleaning rod is connected with the rotating shaft and can circumferentially rotate along the filter residue collecting tank under the driving of the rotating shaft; so as to be convenient for uninterruptedly clear up the impurity that the filter residue bed was filtered down to discharge through filter residue discharge port, sediment discharge channel in proper order, prevent that impurity from piling up in the filter residue collecting vat and influencing the filter effect.
One end of the rotating shaft is positioned in the harvesting bed, the other end of the rotating shaft penetrates through the filter residue bed and then is connected with the rotating wheel, the central axes of the rotating shaft, the filter residue bed and the harvesting bed are coincident, the rotating wheel is used for providing driving force for the rotating shaft, and the filter residue collecting tank is used for collecting impurities filtered out from microalgae cells and culture solution.
Preferably, the residue discharge outlet is one or more openings of suitable shape to facilitate the discharge of impurities to a designated area.
More preferably, the filter residue discharge outlet is 2 circular holes and is symmetrically distributed on the bottom surface of the filter residue collecting tank, so that the discharge speed and efficiency of filter residues can be effectively improved.
Further, the harvesting system further comprises a cleaning water spray pipe, the cleaning water spray pipe is positioned in the harvesting bed, water spray holes are formed in the cleaning water spray pipe, the jet flow direction of the water spray holes faces the harvesting bed, and an inclination angle is formed between the jet flow direction of the water spray holes and the direction of the bed surface of the harvesting bed or the flowing direction of microalgae cells. The cleaning spray pipe is mainly used for cleaning and flushing foreign matters such as nutrient salts, flora and the like on the surfaces of microalgae cells on the bed surface of the harvesting bed, and flushing the microalgae cells to the microalgae mud output port to be discharged out of the harvesting bed while cleaning the microalgae cells.
Preferably, the inclination angle between the jet direction of the water spraying hole and the flow direction of the harvesting bed surface or the microalgae cells is 45-90 degrees.
Further, the harvesting system further comprises a clean water tank, the clean water tank is arranged outside the harvesting system, the clean water tank is connected with one end of the cleaning water spray pipe, the other end of the cleaning water spray pipe is located in the harvesting bed, clean water in the clean water tank is salt-containing clean water which does not contain salt or contains the same concentration as culture solution, when algae cells containing the glucosinolates are harvested, the salt-containing clean water which contains the same concentration as the culture solution is required to be used as cleaning solution, the glucosinolates in the algae cells are prevented from being secreted into the cleaning solution, and the loss of the glucosinolates is caused.
Further, the axis of rotation is hollow structure, and the one end of axis of rotation is located the bed of gathering inside, and the other end passes the filter residue bed and rotates the wheel and be connected back again with the fresh water tank intercommunication, one end or both ends of washing spray pipe all are located the part intercommunication in the bed of gathering with the axis of rotation, and wash the spray pipe and can rotate along with the axis of rotation to spray out the washing water to the surface of the bed of gathering.
Further, the harvesting system further comprises a support frame, a clear water pump and a clear water input port, wherein the clear water tank is fixed on the support frame and is provided with the clear water input port, the rotating shaft is connected with the clear water tank through a dynamic sealing component, and the clear water input port is connected with the clear water pump.
Preferably, the dynamic sealing component comprises a dynamic sealing structure of a packing seal and a mechanical seal.
Further, the harvesting system further comprises a culture solution collecting tank, wherein the culture solution collecting tank is arranged at the lower part of the bed surface of the harvesting bed and used for collecting liquid filtered from the harvesting bed, an algae mud output port formed at the lower end of the harvesting bed penetrates through the bottom surface of the culture solution collecting tank, and the algae mud output port is in sealing connection with the contact part of the bottom surface of the culture solution collecting tank, so that the culture solution in the culture solution collecting tank is prevented from being mixed with microalgae cells again after being leaked.
Microalgae cells and culture solution which are filtered by the filter residue bed to remove impurities enter the bed surface of the harvesting bed, the culture solution and the microalgae cells are rapidly separated in the flowing process, the microalgae cells are collected and then discharged from an algae mud output port at the bottom of the harvesting bed, and the culture solution enters a culture solution collecting tank through a membrane hole filtering structure on the bed surface of the harvesting bed.
Further, the lower part of the culture solution collecting tank is provided with a waste liquid outlet, so that the culture solution in the culture solution collecting tank is conveniently and uniformly discharged after being collected.
Further, the culture-harvesting integrated system further comprises a liquid feeding pump and a liquid feeding valve, and the culture system, the liquid feeding pump, the liquid feeding valve and the liquid feeding pipe are sequentially connected.
Further, the culture-harvesting integrated system further comprises a reflux pump and a liquid outlet valve, wherein the waste liquid outlet, the reflux pump and the liquid outlet valve are sequentially connected to treat and discharge the collected waste liquid, and the separated microalgae cells are used for the next treatment.
Secondly, the application provides a culture-harvesting method of microalgae cells, which comprises the following steps:
(1) Firstly, photosynthetic culture is carried out on microalgae cells through a culture system, then the microalgae cells and culture solution are distributed on the bed surface of a filter residue bed, and impurities in the microalgae cells and the culture solution are filtered through a filtering structure of the bed surface of the filter residue bed;
(2) The microalgae cells and the culture solution which are filtered to remove impurities in the step (1) enter the bed surface of the harvesting bed, so that the culture solution and the microalgae cells are rapidly separated, and the microalgae cells are collected and discharged from an algae mud output port at the bottom of the harvesting bed.
Preferably, in the culture-harvesting method of the microalgae cells, the microalgae cells are spirulina.
Finally, the application provides a method for culturing and harvesting spirulina for synthesizing glyceroglycosides (GG), which comprises the following steps:
(1) Firstly, a liquid feeding pump, a liquid inlet valve, a reflux pump and a liquid outlet valve are all in a closed state, and then culture solution and spirulina are inoculated into a photoreactor;
(2) Starting a stirrer and a light source, introducing carbon source gas through an air inlet pipe, enabling spirulina to absorb the carbon source under photosynthesis and generate oxygen, releasing the oxygen into the culture solution, separating the oxygen from the culture solution under the stirring action of the stirrer, and discharging the oxygen to the outside of the photoreactor from an air outlet;
(3) After the concentration of the spirulina reaches a set value, stopping the introduction of illumination and carbon source gas, starting a liquid feeding pump, a liquid feeding valve, a reflux pump and a liquid discharging valve, enabling the spirulina and the culture solution to sequentially pass through the liquid feeding pump and the liquid feeding valve and enter a liquid feeding pipe, uniformly distributing the spirulina and the culture solution to the bed surface of a filter residue bed through a liquid distribution hole by the liquid feeding pipe, filtering impurities in the spirulina and the culture solution through a membrane hole filtering structure, collecting the impurities in a filter residue collecting tank, removing the impurities from a filter residue outlet by a filter residue cleaning rod into a filter residue channel, finally cleaning a collecting system, and enabling the spirulina and the culture solution with the filtered impurities to enter a collecting bed;
(4) The spirulina and the culture solution which are filtered to remove impurities in the step (5) enter the harvesting bed surface, meanwhile, salt-containing clean water which is arranged in the clean water tank and has the same concentration as the culture solution enters the cleaning spray pipe after passing through the hollow rotating shaft, the clean water is sprayed out from the water spraying holes of the cleaning spray pipe along with the rotation of the rotating shaft, the spirulina on the harvesting bed surface is cleaned, the jet flow direction of the water spraying holes and the flowing direction of the spirulina form an inclined angle of 45-90 degrees, so that the culture solution and the spirulina are rapidly separated, and the spirulina is discharged from the algae mud output port at the bottom of the harvesting bed after being collected; the culture solution enters the culture solution collecting tank through the membrane hole filtering structure on the bed surface, then enters the reflux pump from the waste liquid outlet, and then is used for culturing the spirulina again after passing through the liquid outlet valve.
Compared with the prior art, the application has the beneficial effects that:
(1) The application designs the air inlet pipe and the stirrer of the culture system integrally, wherein the stirrer is provided with the air inlet pipe and the air outlet hole, and carbon source-containing gas enters the culture solution from the air outlet hole after passing through the air inlet pipe, so that the ventilation can be realized, the stirring can be carried out at the same time, the entering gas is more uniformly mixed into the culture solution, and microalgae cells in the photoreactor are more uniform in the aspects of receiving illumination, nutrition supply and carbon source gas absorption.
(2) Compared with the traditional recovery bed, the application adopts the structure of stacking the filter residue bed and the recovery bed up and down, saves the occupied area of equipment arrangement, timely cleans out large-particle impurities filtered by the filter residue bed through the cleaning rod, and improves the capability of the device for treating wastes. In addition, the bed surface structure of the recovery bed adopts a variable-camber horn funnel-shaped structure with the inclination angle being continuously or stepwisely changed from 0 to 90 degrees, and is characterized in that: when microalgae cells and culture solution just enter the bed surface, the gradient of the bed surface is slower, the separation area of the bed surface is larger, the rapid separation of a large amount of culture solution is facilitated, along with the rapid separation of the culture solution, the harvesting bed surface is rapidly contracted, the inclination angle of the bed surface is also continuously increased, the flow and rapid discharge of the microalgae cells from the harvesting bed are facilitated, the microalgae cell accumulation problem is prevented, and the harvesting efficiency is greatly improved.
(3) The culture-harvesting integrated system of the microalgae cells is very suitable for extracting the glyceroglycosides in the spirulina, the spirulina absorbs carbon dioxide through photosynthesis and generates oxygen in the culture process, the oxygen is released into a liquid culture medium, the oxygen is separated from a liquid layer along with ventilation and stirring, and is discharged to the outside of a photoreactor from an exhaust port, so that the normal growth of the spirulina and the accumulation of the glyceroglycosides in the spirulina cells are well ensured, and after the culture is finished, the spirulina cells containing the glyceroglycosides can be rapidly separated from a culture solution when the spirulina cells are harvested, so that the efficient culture and harvesting of the spirulina cells are realized.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application.
FIG. 1 shows an integrated system for culturing and harvesting microalgae cells according to the application.
Fig. 2 is a schematic diagram of the recovery system of the present application.
Fig. 3 is a schematic diagram of the structure of the filter bed and the recovery bed in example 1.
Fig. 4 is a schematic diagram of the structure of the filter bed and the recovery bed in example 2.
FIG. 5 is a schematic diagram showing the sealing connection of the clean water tank and the rotary shaft filler.
The label designations in the drawings represent: 1-photoreactor, 2-microalgae cells, 3-culture solution, 4-intake pipe, 5-carbon source-containing gas, 6-stirrer, 7-exhaust port, 8-light source, 9-harvesting system, 10-liquid feeding pump, 11-liquid inlet valve, 12-reflux pump, 13-liquid outlet valve, 14-liquid inlet pipe, 15-filter residue bed, 16-harvesting bed, 17-cleaning spray pipe, 18-culture solution collecting tank, 19-algae mud outlet, 20-waste liquid outlet, 21-clean water pump, 22-filter residue collecting tank, 23-filter residue cleaning rod, 24-clean water tank, 25-rotating wheel, 26-filter residue outlet, 27-rotating shaft, 28-supporting frame, 29-dynamic sealing component, 30-clean water inlet and 31-slag discharging channel.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
As described in the background art, the existing microalgae cell culture and harvesting methods and systems still have the problems of complex culture process, high cost, low harvesting efficiency and the like, so the application provides a microalgae cell culture-harvesting integrated system and method, and the application is further described below with reference to the accompanying drawings and detailed description.
Example 1
As shown in fig. 1-5, a microalgae cell culture-harvesting integrated system comprises a culture system, a harvesting system 9, a liquid feeding pump 10, a liquid inlet valve 11, a reflux pump 12 and a liquid outlet valve 13; the culture system is used for culturing algae cells, and the harvesting system 9 is used for separating microalgae cells and culture solution.
The culture system comprises: a photoreactor 1, an air inlet pipe 4, a stirrer 6, an air outlet 7 and a light source 8.
The microalgae cells 2 and the culture solution 3 are mixed together and are positioned in the photoreactor 1, the photoreactor 1 is cylindrical, one end of an air inlet pipe 4 is inserted into the culture solution 3, the other end of the air inlet pipe is connected with an air source containing carbon source gas 5, and the carbon source gas 5 enters the culture solution 3 through the air inlet pipe 4 to provide carbon elements for the photosynthetic growth of the microalgae cells.
The stirrer 6 is arranged in the photoreactor 1, the exhaust port 7 is arranged at the upper part of the liquid level of the culture solution, and the light source 8 is an LED arranged in the photoreactor 1.
The recovery system includes: the liquid feeding pump 10, the liquid feeding valve 11 and the liquid feeding pipe 14 are sequentially connected, the filter residue bed 15 is of a truncated cone-shaped structure with the diameter of the lower end face larger than that of the upper end face, the side face of the filter residue bed forms a bed surface of a filtering structure, the liquid feeding pipe 14 is annularly fixed at the upper end of the side face of the truncated cone, and liquid distribution holes with jet flow directions facing the filter residue bed are formed in the liquid feeding pipe 14; the bed surface of the filter residue bed 15 is a membrane hole filtering structure and is made of membrane hole 50-mesh soft filter cloth.
The filter residue bed 15 is arranged above the harvesting bed 16, and an algae mud output port 19 is formed at the lower end of the harvesting bed 16; the periphery of the lower end face of the filter residue bed 15 is provided with a filter residue collecting tank 22, and the bottom surface of the filter residue collecting tank 22 is provided with a filter residue outlet 26, and a slag discharging channel 31 is connected with the filter residue outlet 26.
The residue collecting tank 22 is provided therein with a residue cleaning rod 23 which is connected to the rotation shaft 27 and is capable of rotating circumferentially along the residue collecting tank 22 by the rotation shaft 27.
The harvesting bed 16 is of a funnel-shaped structure, the side wall of the harvesting bed forms a bed surface of a filtering structure, the inclination angle of the bed surface is continuously changed from 0 to 90 degrees, microalgae cells 2 and culture solution 3 for filtering impurities through the filter residue bed 15 enter the bed surface of the harvesting bed 16, in the flowing process, the culture solution and the microalgae cells 2 are rapidly separated, algae mud formed after the microalgae cells 2 are gathered is discharged from an algae mud outlet 19 at the bottom of the harvesting bed 16, the culture solution 3 enters a culture solution collecting tank 18 through a membrane hole filtering structure on the bed surface, and the bed surface of the harvesting bed 16 is of a membrane hole filtering structure and is made of membrane hole 300-mesh hard filter cloth.
The water spraying cleaning pipe 17 is arranged in the harvesting bed 16, the rotating shaft 27 is of a hollow structure, one end of the rotating shaft 27 is positioned in the harvesting bed 16, the other end of the rotating shaft 27 penetrates through the filter residue bed 15 and then is connected with the rotating wheel 25, central axes of the rotating shaft 27, the filter residue bed 15 and the harvesting bed 16 coincide, the water spraying cleaning pipe 4 is communicated with a part of the rotating shaft 27 extending into the harvesting bed 16, a plurality of water spraying holes are formed in the water spraying cleaning pipe 17, the jet flow direction of the water spraying holes faces the harvesting bed 3, and an inclined angle of 45 degrees is formed between the jet flow direction of the water spraying holes and the flowing direction of microalgae cells; the other end of the rotating shaft 27 is communicated with the clean water tank 24, the clean water tank 24 is arranged above the rotating shaft 27 and fixed on the supporting frame 28, a clean water input port 30 is arranged on the clean water tank 24, the rotating shaft 27 and the clean water tank 24 are connected through a dynamic sealing component 29, the clean water input port 30 is connected with the clean water pump 21, and the dynamic sealing component 29 is of a dynamic sealing structure with a packing seal.
The rotating wheel 25 is sleeved on the rotating shaft 27 to provide driving force for the rotation of the rotating shaft 27, clean water in the clean water tank 24 enters the cleaning water spray pipe 17 after passing through the hollow rotating shaft 27, and the clean water is sprayed out of the water spray holes of the cleaning water spray pipe 17 along with the rotation of the rotating shaft 27 to uniformly clean microalgae cells on the bed surface of the harvesting bed 16, and the microalgae cells are flushed to the algae mud output port 19 to be discharged out of the harvesting bed 16 while the microalgae cells are cleaned.
The cleaning water spray pipe 17 is provided with a plurality of water spray holes, the jet flow direction of the water spray holes faces the harvesting bed 16, and the water spray holes and the direction of the algae mud flow form an inclined angle of 45 degrees.
The culture solution collecting tank 18 is arranged at the lower part of the bed surface of the harvesting bed 16, an algae mud output port 19 formed at the lower end of the harvesting bed 16 penetrates through the bottom surface of the culture solution collecting tank 18, and the algae mud output port 19 is in sealing connection with the contact part of the bottom surface of the culture solution collecting tank 18.
The lower part of the culture solution collecting tank 18 is provided with a waste solution outlet 20, the waste solution outlet 20 is connected with the reflux pump 12 and the liquid outlet valve 13 to treat and discharge the collected waste solution, and the separated microalgae cells are used for the next treatment.
Example 2
A microalgae cell harvesting system, which is similar to example 1, and differs from the system in that: (1) The bed surface of the filter residue bed 15 is made of hard film materials with film holes of 80 meshes.
(2) The bed surface of the recovery bed 16 is made of soft filter cloth with membrane holes of 100 meshes.
(3) The gradient change of the inclination angle alpha of the bed surface of the recovery bed 16 relative to the horizontal plane is divided into 4 gradients, which are alpha in turn 1 ＝5°、α 2 ＝15°、α 3 ＝45°、α 4 ＝85°。
Example 3
A microalgae cell culture-harvesting integrated system is similar to that of example 1, and is different in that: the difference is that: (1) The bed surface of the filter residue bed 15 is made of a hard film material with film holes of 30 meshes.
(2) The bed surface of the recovery bed 16 is made of soft filter cloth with 400 meshes of membrane holes.
(3) The jet direction of the water spraying holes on the cleaning water spraying pipe 17 is inclined at an angle of 90 degrees with the flowing direction of the algae mud. The light source 8 is natural sunlight.
(4) The dynamic seal member 29 is a dynamic seal structure of a mechanical seal.
(5) The air inlet pipe and the stirrer are integrally designed: the stirrer is provided with an air inlet pipeline and an air outlet hole, and carbon source-containing gas 5 enters the culture solution 3 from the air outlet hole after passing through the air inlet pipeline.
Example 4
A microalgae cell culture-harvesting integrated system is similar to that of example 1, and is different in that: (1) The bed surface of the filter residue bed 15 is made of soft filter cloth material with membrane holes of 100 meshes.
(2) The bed surface of the recovery bed 16 is made of soft filter cloth with membrane holes of 600 meshes.
Example 5
A method for cultivating-harvesting spirulina capable of synthesizing glyceroglycoside (GG) using the integrated system of example 1, comprising the steps of:
(1) Firstly, the liquid feeding pump 10, the liquid inlet valve 11, the reflux pump 12 and the liquid outlet valve 13 are all in a closed state, and then the culture solution 3 and the spirulina 2 are inserted into the photoreactor 1; the culture solution 3 is an f/2 culture solution, the culture solution 3 comprises nutrient substances such as water, nitrogen, phosphorus, calcium, magnesium, iron, trace metal nutrient salts and the like, and the carbon source-containing gas contains CO 2 Is a gas of (2);
(2) The stirrer 6 and the light source 8 are started, and CO is introduced through the air inlet pipe 4 2 The gas 5, the spirulina 2 has the basic conditions capable of photosynthesis growth and synthesis of GG in the spirulina, then the synthesis of GG is started, in the GG generation process, the spirulina 2 absorbs carbon dioxide under photosynthesis and generates oxygen, the oxygen is released into the culture solution 3, and along with the stirring action of the stirrer, the oxygen is separated from the culture solution 3 and is discharged to the outside of the photoreactor 1 from the exhaust port 7, so that the smooth synthesis process of GG is ensured;
(3) With the continuous GG synthesis reaction, the GG content in the spirulina 2 is gradually increased, (3) after the content of the spirulina 2 in the culture solution 3 reaches 2g/L, stopping the illumination and the introduction of the carbon-containing source gas 5, starting a liquid feeding pump 10, a liquid feeding valve 11, a reflux pump 12 and a liquid outlet valve 13 to enable the spirulina 2 and the culture solution 3 in the photoreactor 1 to enter a liquid feeding pipe 14 through the liquid feeding valve 11, uniformly distributing the spirulina 2 and the culture solution 3 to the side surface of a filter residue bed 15 through liquid distribution holes on the liquid feeding pipe 14 by the liquid feeding pipe 1, filtering impurities in the spirulina 2 and the culture solution 3 through a membrane hole filtering structure, collecting the impurities in a filter residue collecting tank 22, and removing the impurities from a filter residue outlet 26 by a filter residue cleaning rod 23 to enable the spirulina 2 and the culture solution 3 with the filtered impurities to enter a harvesting bed;
(4) The spirulina 2 and the culture solution 3 which are filtered to remove impurities in the step (5) enter the bed surface of the harvesting bed 16, meanwhile, clear water in the clear water tank 24 enters the cleaning water spray pipe 17 after passing through the hollow rotating shaft 25, and the clear water is sprayed out from the water spray holes of the cleaning water spray pipe 17 along with the rotation of the rotating shaft 25 to uniformly clean the spirulina on the bed surface of the harvesting bed 16, the jet flow direction of the water spray holes forms an inclined angle of 45-90 degrees with the flowing direction of the spirulina, so that the culture solution 3 and the spirulina 2 are rapidly separated, and the spirulina 2 is discharged from the algae mud output port 19 at the bottom of the harvesting bed 16 after being gathered for separation, extraction and purification of the next GG; the culture medium 3 enters the culture medium collecting tank 18 through a membrane hole filtering structure on the bed surface, then enters the reflux pump 12 from the waste liquid outlet 20, and then passes through the liquid outlet valve 13 to be used for culturing the spirulina 2 again.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims (27)

1. A culture-harvesting integrated system of microalgae cells is characterized in that: the integrated system consists of a culture system and a harvesting system; the culture system comprises: the device comprises a photoreactor, an air inlet pipe, a stirrer and a light source;
one end of the air inlet pipe is inserted into the culture solution of the photoreactor; the stirrer is arranged in the photoreactor; the light source is arranged inside and/or outside the photoreactor;
the harvesting system consists of a filter residue bed and a harvesting bed, wherein the filter residue bed is arranged above the harvesting bed, the central axes of the filter residue bed and the harvesting bed are coincident, and microalgae cells enter the filter residue bed after finishing culturing in the photoreactor; the lower end of the harvesting bed forms an algae mud output port; the filter residue bed is of a truncated cone-shaped structure with the diameter of the lower end face larger than that of the upper end face, and the side face of the truncated cone forms a filter residue bed surface of the filter structure; the harvesting bed is of a variable-curved horn funnel-shaped structure, the side wall of the harvesting bed forms a harvesting bed surface of a filtering structure, and the inclination angle alpha of the harvesting bed surface relative to the horizontal plane is continuously changed or is changed in a gradient way from 0 degree to 90 degrees;
the harvesting system further comprises a liquid inlet pipe, the liquid inlet pipe is connected with the culture system, the liquid inlet pipe is annularly fixed at the upper end of the surface of the filter residue bed, and a plurality of liquid distribution holes with jet flow directions facing the filter residue bed are formed in the liquid inlet pipe;
the filter residue bed surface is a membrane hole filtering structure which is made of a hard or soft filtering material with membrane holes of 10-100 meshes;
the harvesting bed surface is a membrane hole filtering structure which is made of a hard or soft filtering material with 100-800 meshes of membrane holes;
the harvesting system also comprises a filter residue collecting tank, a filter residue cleaning rod, a rotating wheel, a filter residue discharge port, a slag discharge channel and a rotating shaft; the filter residue collecting tank is arranged on the periphery of the lower end face of the filter residue bed and is tightly connected with the filter residue bed, the filter residue discharge port is arranged in the filter residue collecting tank, the residue discharge channel is connected with the filter residue discharge port, the filter residue cleaning rod is connected with the rotating shaft and can circumferentially rotate along the filter residue collecting tank under the driving of the rotating shaft, one end of the rotating shaft is positioned in the collecting bed, the other end of the rotating shaft penetrates through the filter residue bed and is connected with the rotating wheel, and the central axes of the rotating shaft, the filter residue bed and the collecting bed coincide;
the harvesting system further comprises a culture solution collecting tank, the culture solution collecting tank is arranged at the lower part of the bed surface of the harvesting bed, an algae mud output port formed at the lower end of the harvesting bed penetrates through the bottom surface of the culture solution collecting tank, and the algae mud output port is in sealing connection with the contact part of the bottom surface of the culture solution collecting tank;
the lower part of the culture solution collecting tank is provided with a waste solution outlet;
the integrated system further comprises a liquid feeding pump, a liquid inlet valve, a reflux pump and a liquid outlet valve, wherein the culture system, the liquid feeding pump, the liquid inlet valve and the liquid inlet pipe are sequentially connected, and the waste liquid outlet, the reflux pump and the liquid outlet valve are sequentially connected.
2. The integrated culture-harvesting system of microalgae cells of claim 1, wherein: the air inlet pipe and the stirrer are integrally designed: the stirrer is provided with an air inlet pipeline and an air outlet hole, and carbon source-containing gas enters the culture solution from the air outlet hole after passing through the air inlet pipeline.
3. The integrated culture-harvesting system of microalgae cells of claim 1, wherein: the culture system also comprises an exhaust port, and the exhaust port is arranged at the upper part of the liquid level of the culture solution in the photoreactor.
4. The integrated culture-harvesting system of microalgae cells of claim 1, wherein: the photoreactor comprises a closed tubular reactor, a flat plate reactor, a cylindrical photoreactor or an open runway pool.
5. The integrated culture-harvesting system of microalgae cells of claim 1, wherein: the shape of the stirrer comprises a spiral shape and a paddle shape.
6. The integrated culture-harvesting system of microalgae cells of claim 1, wherein: the light source comprises natural sunlight and an artificial light source.
7. The integrated culture-harvesting system of microalgae cells of claim 6, wherein: the artificial light source comprises an LED, a fluorescent lamp and a mercury lamp.
8. The integrated culture-harvesting system of microalgae cells of claim 1, wherein: the harvesting system further comprises a cleaning water spray pipe, the cleaning water spray pipe is positioned in the harvesting bed, a water spray hole is formed in the cleaning water spray pipe, the jet flow direction of the water spray hole faces the harvesting bed, and the jet flow direction of the water spray hole is inclined with the direction of the bed surface of the harvesting bed or the flowing direction of microalgae cells.
9. The integrated culture-harvesting system of microalgae cells of claim 8, wherein: the inclination angle is 45-90 degrees.
10. The integrated culture-harvesting system of microalgae cells of claim 1, wherein: the harvesting system further comprises a clean water tank, the clean water tank is arranged outside the harvesting system and connected with one end of the cleaning water spray pipe, and the other end of the cleaning water spray pipe is located in the harvesting bed.
11. The integrated culture-harvesting system of microalgae cells of claim 8, wherein: the harvesting system further comprises a dynamic sealing component, the rotating shaft is of a hollow structure, one end of the rotating shaft is located inside the harvesting bed, the other end of the rotating shaft penetrates through the filter residue bed to be connected with the rotating wheel and then is communicated with the clean water tank, and one end or two ends of the cleaning water spray pipe are communicated with the part of the rotating shaft located in the harvesting bed and can rotate along with the rotating shaft.
12. The integrated microalgae cell culture and harvesting system of claim 11, wherein: the harvesting system further comprises a support frame, a clear water pump and a clear water input port, wherein the clear water tank is fixed on the support frame, the clear water input port is arranged on the clear water tank, and the clear water pump is connected with the clear water input port.
13. The integrated microalgae cell culture and harvesting system of claim 11, wherein: the dynamic sealing component comprises a dynamic sealing structure of a packing seal and a mechanical seal.
14. The integrated microalgae cell culture and harvesting system of claim 11, wherein: the gradient change of the inclination angle alpha of the harvesting bed surface relative to the horizontal plane is divided into 4 gradients: 5 °, 15 °, 45 °, 85 °.
15. A method for culturing-harvesting microalgae cells using the integrated culturing-harvesting system of any one of claims 1-14, characterized in that: the method comprises the following steps:
(1) Firstly, photosynthetic culture is carried out on microalgae cells through a culture system, then the microalgae cells and culture solution are distributed on a filter residue bed surface, and impurities in the microalgae cells and the culture solution are filtered through a filter structure of the filter residue bed surface;
(2) The microalgae cells and the culture solution which are filtered to remove impurities in the step (1) enter the bed surface of the harvesting bed, so that the culture solution and the microalgae cells are rapidly separated, and the microalgae cells are collected and discharged from an algae mud output port at the bottom of the harvesting bed.
16. The method for culture-harvesting of microalgae cells of claim 15, wherein: the microalgae cells are spirulina.
17. A method for cultivating-harvesting spirulina capable of synthesizing glyceroglycosides using the integrated system of claim 11, characterized in that: the method comprises the following steps:
(1) Firstly, a liquid feeding pump, a liquid inlet valve, a reflux pump and a liquid outlet valve are all in a closed state, and then culture solution and spirulina are inoculated into a photoreactor;
(2) Starting a stirrer and a light source, introducing carbon source gas through an air inlet pipe, enabling spirulina to absorb the carbon source under photosynthesis and generate oxygen, releasing the oxygen into the culture solution, separating the oxygen from the culture solution under the stirring action of the stirrer, and discharging the oxygen to the outside of the photoreactor from an air outlet;
(3) After the concentration of the spirulina reaches a set value, stopping the introduction of illumination and carbon source gas, starting a liquid feeding pump, a liquid feeding valve, a reflux pump and a liquid discharging valve, enabling the spirulina and the culture solution to sequentially pass through the liquid feeding pump and the liquid feeding valve and enter a liquid feeding pipe, uniformly distributing the spirulina and the culture solution to the bed surface of a filter residue bed through a liquid distribution hole by the liquid feeding pipe, filtering impurities in the spirulina and the culture solution through a membrane hole filtering structure, collecting the impurities in a filter residue collecting tank, removing the impurities from a filter residue outlet by a filter residue cleaning rod into a filter residue channel, finally cleaning a collecting system, and enabling the spirulina and the culture solution with the filtered impurities to enter a collecting bed;
(4) The spirulina and the culture solution which are filtered to remove impurities in the step (5) enter the harvesting bed surface, meanwhile, salt-containing clean water which is arranged in the clean water tank and has the same concentration as the culture solution enters the cleaning spray pipe after passing through the hollow rotating shaft, the clean water is sprayed out from the water spraying holes of the cleaning spray pipe along with the rotation of the rotating shaft, the spirulina on the harvesting bed surface is cleaned, the jet flow direction of the water spraying holes and the flowing direction of the spirulina form an inclined angle of 45-90 degrees, so that the culture solution and the spirulina are rapidly separated, and the spirulina is discharged from the algae mud output port at the bottom of the harvesting bed after being collected; the culture solution enters the culture solution collecting tank through the membrane hole filtering structure on the bed surface, then enters the reflux pump from the waste liquid outlet, and then is used for culturing the spirulina again after passing through the liquid outlet valve.
18. The method as claimed in claim 17, wherein: the carbon source gas contains CO 2 Is a gas of (a) a gas of (b).
19. The method as claimed in claim 17, wherein: the culture solution is seawater culture solution.
20. The method as claimed in claim 19, wherein: the culture solution is f/2 culture solution.
21. The method as claimed in claim 17, wherein: the culture solution is fresh water culture solution.
22. The method as claimed in claim 21, wherein: the culture solution is BG.
23. The method as claimed in claim 17, wherein: the culture solution is an improved acidic culture solution.
24. The method as claimed in claim 17, wherein: the culture solution is an improved alkaline culture solution.
25. The method as claimed in claim 24, wherein: the culture solution is Zarok alkaline culture solution.
26. The method as claimed in claim 17, wherein: the culture solution is MC green algae culture solution.
27. The method as claimed in claim 26, wherein: the nutrient substances in the culture solution comprise water, nitrogen, phosphorus, calcium, magnesium, iron and trace metal nutrient salts.
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