CN217033977U - Full-automatic flowing water immunodetection system - Google Patents

Abstract

The utility model discloses a running water full-automatic immunodetection system, which comprises a to-be-detected area, a bar code identification area, a conveying area, a plurality of detection areas, a homing area, a controller and a power supply, wherein each detection area consists of a sample introduction/recovery unit, a conveying belt unit, a shaking unit, a batten stacking unit, a reaction cup transfer unit, a sample arm, a reagent bin unit, an incubation unit, a liquid path unit, a waste recovery unit and a detection unit; by providing the flow full-automatic immunodetection system and the detection method thereof, the system can adapt to various sample types, multi-index multithreading and flow asynchronous detection, greatly improve the detection speed and the application flexibility, can adapt to the application requirements of more customers, simultaneously reduce the detection waiting time and further improve the use convenience.

Description

Full-automatic flowing water immunodetection system

Technical Field

The utility model relates to a full-automatic immunodetection system, in particular to a running water full-automatic immunodetection system.

Background

The device is a POCT full-automatic quantitative system depending on instruments, realizes the identification of various sample types and the pretreatment possibly needed by the samples through a unique sample introduction unit, and completes the addition of the samples and the whole dry-type fluorescent reaction process through a sample addition unit and a consumable unit (a reaction cup transfer unit and a reaction plate strip stacking unit) to finally complete the detection. The POCT rapid detection system realizes the automatic, informatization, integration, individuation and modularization rapid detection of POCT, and various sample types can be directly detected on a computer, thereby meeting the development requirement of informatization of hospitals and the multipurpose requirement and quality guarantee of clinical emergency treatment.

He has the following characteristics:

the method can support direct on-machine detection of various samples, an original tube is directly on-machine detected without opening a cover, biological safety risks are avoided, and complicated manual on-machine pretreatment of the samples is not needed;

the temperature is controllable in the whole reaction process;

the device can simultaneously detect multiple indexes, and the items cover rapid detection of other cardiovascular diseases, infectious diseases, female health, infection, embolism and the like to obtain demonstration application.

The device is a modularized device, can be used independently or online, has expandability and can be used in an interconnected way with devices of the same type (meeting the interconnection requirement). The device is an immunodetection assembly line which is few in the industry, the detection speed is higher than that of a single dry type immunoassay analyzer with the speed equal to that of the dry type immunoassay analyzer exceeding the same speed, the JS3000 on-machine detection device is ensured to adapt to various sample types and multiple indexes, and the whole-process temperature control is reflected, so that the detection speed and the application flexibility are greatly improved, the application requirements of more customers can be adapted, meanwhile, the waiting time of the customers is reduced, and the use convenience is further improved.

Disclosure of Invention

The utility model aims to provide a full-automatic running water immunity detection system and a detection method thereof aiming at the defects of the prior art.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a running water full-automatic immunity detection system comprises a to-be-detected area, a bar code identification area, a conveying area, a plurality of detection areas, a homing area, a controller and a power supply, wherein each detection area consists of a sample introduction/recovery unit, a conveying belt unit, a shaking unit, a batten stacking unit, a reaction cup transfer unit, a sample arm, a reagent bin unit, an incubation unit, a liquid path unit, a waste recovery unit and a detection unit;

the test tube is characterized in that a plurality of samples to be tested are placed in the area to be tested, the samples to be tested are placed in the test tube on the test tube support, identification barcodes corresponding to the information of the samples to be tested are arranged on the test tube, the area to be tested is connected with the sample injection/recovery unit of the detection area, the arrangement area and the detection area through the conveying area, one side of the sample injection/recovery unit is connected with the conveying area and used for transmitting the samples to be tested and the tested samples between the conveying area and the detection area, the other side of the sample injection/recovery unit is provided with a conveying belt unit and used for transmitting the samples to be tested to the shaking unit one by one, the barcode identification area is arranged on a channel of the conveying area, the conveying belt unit is adjacent to the shaking unit, a sample arm is arranged above the shaking unit, a batten stacking unit is arranged on one side of the sample arm, a batten stacking unit is provided with a batten conveying mechanism, and a reaction cup transferring unit is arranged above the batten stacking unit, the reaction cup transfer unit is provided with a reaction cup conveying device, the reaction cup conveying device is adjacent to the reagent arm, and a waste recovery unit is arranged below the reaction cup transfer unit; the reagent arm one side is equipped with reagent storehouse unit, be connected with the liquid way unit on the reagent arm, reagent arm opposite side is equipped with incubation unit, incubation unit one side is equipped with the detecting element, be equipped with push mechanism between lath stack unit, incubation unit and the detecting element, push mechanism is the push rod, through push rod's removal, with lath from lath stack unit propelling movement to incubation unit and detecting element, sample arm and reagent arm link to each other with the liquid way unit.

Furthermore, a sensor and a push rod are arranged between the conveying area and the placing area as well as between the sample injection/recovery unit, the sensor is used for detecting whether a support bearing a sample is in place or not, the push rod is started after the support is in place, the support is pushed into the unit, pull pieces are arranged on two sides of a channel in the area to be detected, the placing area and the sample injection/recovery unit respectively, the pull pieces are telescopic movable pull pieces, each pull piece is provided with an electronic switch, and the pull pieces are retracted to two sides of the channel after the sample support is pushed to a designated position and completely unfolded along guide rails in two sides in the process of starting the stroke movement of pushing and pulling the sample support by the pull pieces after the pull pieces are started.

Further, shake even unit and include test tube clamp, anticreep strip, shake even motor, Z axle motor, Y axle motor, the test tube clamp top is equipped with the anticreep strip, prevents to drop shaking the test tube in the even in-process test tube clamp, shake even motor and test tube clamp swing joint, the test tube clamp shakes even activity along with shaking the rotation of even motor, the Z axle motor passes through belt and runner on the Z axle motor support and shakes even motor swing joint, the belt and runner and Z axle motor swing joint on the Y axle motor support are passed through to the Y axle motor, make the test tube clamp can reciprocate around, realize mentioning the test tube from the support, the action of putting down.

Further, the lath stacking unit comprises a clamping frame, a clamping taking arm, a push rod, a clamping frame code scanner and a clamping frame code scanner support, laths (cards) are placed in the clamping frame, laths corresponding to different detection items are placed in different clamping frames, the clamping frame code scanner is arranged on the rear side of the clamping frame and used for distinguishing items and the number of the laths tested by the laths in the clamping frame, the clamping frame code scanner is arranged on the clamping frame code scanner support, a clamping taking arm support is arranged below the clamping frame code scanner support, the push rod and the clamping taking arm are arranged on the clamping taking arm support, a motor is arranged below the clamping taking arm and used for rotating the clamping taking arm, a clamping seat is arranged on the clamping taking arm, a clamping taking telescopic rod is arranged below the clamping seat, one end of the clamping taking telescopic rod is provided with a taking hook which is a telescopic movable hook, when the laths are taken, the clamping telescopic rod extends forwards along the guide rail at the bottom of the clamping frame, and the taking hook supports the bottom of the clamping frame to extend forwards, the bottom of the clamping frame is provided with a clamping groove, when the clamping hook reaches a clamping groove opening at the bottom of the clamping frame, the clamping hook smoothly slides into the groove to hook and take the lath (clamp) at the bottommost part of the clamping frame, and the height of the clamping hook is consistent with the thickness of the lath (clamp).

Further, the reaction cup transfer unit comprises a drawer, reaction cup holders, grippers, reaction cup holders, movable cup holders, gripper moving units and reaction cup holder guide rails, one or more reaction cup holders are arranged in the drawer, empty reaction cups are stored in the reaction cup holders, the grippers are arranged above the reaction cup holders, the grippers are connected with the gripper moving units through gripper supports, the reaction cup holders are connected with the reaction cup holder guide rails through the movable cup holders, the gripper moving units comprise gripper supports and movable supports, the movable supports are controlled to move back and forth through motors, the gripper supports are arranged on the movable supports and movably connected with X-direction motors through belts and transmission wheels to control the grippers to move left and right, Z-direction motors are arranged on the gripper supports and movably connected with the grippers through belts and transmission wheels, the automatic detection device comprises a guide rail, a movable cup holder, a reaction cup seat, a reset spring, a guide rail, a motor, a belt and a driving wheel, wherein the guide rail is movably connected with the movable cup holder, the movable cup holder can move along the guide rail, the reaction cup seat is arranged on the movable cup holder and is provided with the reset spring, the gripper is provided with the electromagnetic valve and a gripper, the reaction cup guide rail is arranged on a guide rail support, the guide rail support is movably connected with the reagent arm through the motor, the belt and the driving wheel, the reagent arm is provided with a double-needle support, the double-needle support is provided with a reagent needle and a waste liquid needle, and the reagent needle and the waste liquid needle are connected with a liquid path unit.

Further, the sample arm includes sample needle, agitating unit, movable rod, motor support, armset rotating electrical machines and armset elevator motor, the sample needle sets up on the sample needle file, the sample needle file is connected with the movable rod, be equipped with agitating unit on the movable rod, movable rod and armset motor leg joint, be equipped with armset rotating electrical machines and armset elevator motor on the armset motor support, be equipped with buffer on the sample needle file, buffer is equipped with the top with the sample needle, and the artificial buffering space that sets up prevents that the sample needle from touching when going up and down the activity, and the syringe needle touches and supports, the hard damage of production, DC motor is established on the agitating unit, DC motor is connected with the eccentric plate, be equipped with sample needle via hole on the eccentric plate, sample needle via hole below is equipped with the needle washing pond. The sample needle is lifted through the sample needle through hole, the through hole is arranged on the eccentric plate, and when the eccentric plate is driven by the direct current motor, the sample needle through hole surrounding the sample needle rotates and drives the sample needle to stir; and a needle washing pool below the sample needle is connected with a washing liquid and can wash the outer wall of the sample needle, and an armset rotating motor and an armset lifting motor on the armset motor support are connected with the movable rod through a driving wheel and a belt. The lifting and the rotation of the movable rod are realized through the forward and backward rotation of the motor, the lifting and the rotation of the sample needle seat are driven at the same time, a coded disc is arranged between the movable rod and the arm group rotating motor, and the rotation degree of the sample needle rocker arm is calibrated through the coded disc.

Further, the reagent bin unit comprises a heat radiating fan, heat radiating fins, reagent bottles, a bin body, a heat insulating layer, a window, a bar code machine, a sensor, a refrigerating sheet and an air guiding device, wherein the reagent bottles are distributed in a fan shape and arranged in the reagent tray, the bottom of the reagent tray is connected with a bearing, the bearing is connected with a stepping motor and a stepping sensor, the motor is connected with the bearing to drive the whole bin body to rotate, the reagent tray is provided with a notch corresponding to a bar code on the reagent bottles, the bin body is provided with the window corresponding to the notch, one side of the window is provided with the bar code machine, the bin body is rotated, the bar code machine can read the information of each reagent bottle through the rotation of the bin body, the center of the reagent tray is provided with the heat radiating fins, the heat radiating fan is arranged above the heat radiating fins, the lower part of the bin body is provided with the refrigerating sheet which is connected with the air guiding device, the lower part of the reagent tray is connected with the stepping motor and the stepping sensor, the motor is connected with the bearing and can drive the whole bin body to rotate, a heat preservation layer is arranged outside the bin body and is made of heat preservation cotton, and the bottom of the bin body is connected with the liquid path unit and used for discharging condensed water generated in refrigeration.

Further, the liquid path unit comprises a reagent needle inner and outer cleaning liquid path, a sample needle inner clear water cleaning liquid path, a sample needle outer cleaning liquid path and a waste liquid discharge liquid path;

the reagent needle inner and outer cleaning liquid path comprises a water cleaning barrel, a first pump, a first valve and a first needle washing pool which are sequentially communicated, the reagent needle is arranged in the first needle washing pool, one path of the first valve is connected with a first plunger pump through a reagent needle interface to be used for cleaning the inner wall of the reagent needle inside the reagent needle, the other path of the first valve is connected with the first needle washing pool to be used for cleaning the outer wall of the reagent needle, and the first plunger pump can be used for extracting and discharging reagent solution through the reagent needle;

the clean water cleaning liquid path on the inner side of the sample needle comprises a clean water barrel, a second pump, a second valve, a second plunger pump and a fourth valve which are sequentially communicated, the second plunger pump is connected with the interior of the sample needle through a sample needle interface, when the fourth valve is gated, the clean water cleaning liquid path on the inner side of the sample needle cleans the inner wall of the reagent needle, and the second plunger pump can extract and discharge a sample solution through the sample needle;

the sample needle outside lotion cleaning solution way is including the lotion bucket, third pump and the third valve that communicate in proper order, the sample needle is located the second and is washed the needle pond, and the second is washed the needle pond outer wall to the third valve connection, and when the third valve was selected, the sample needle outside lotion cleaning solution way cleaned the outer wall of sample needle.

The liquid path system further comprises a condensate and waste liquid discharge path, the condensate and waste liquid discharge path comprises a waste liquid needle, a fifth pump and a fifth valve which are sequentially communicated, one path of the fifth valve is connected with the reagent disc through a reagent disc interface, the other path of the fifth valve is connected with the waste liquid needle through a waste liquid needle interface, and the fifth pump is used for discharging the condensate in the reagent disc and the waste liquid sucked by the waste liquid needle into the waste liquid barrel.

The bottom of the first needle washing pool is connected with a fourth pump, and the fourth pump is used for discharging the waste liquid in the first needle washing pool to a waste liquid barrel.

And the bottom of the second needle washing pool is connected with a sixth pump, and the sixth pump is used for discharging the waste liquid in the second needle washing pool to a waste liquid barrel.

And a filter and a connecting joint are also arranged between the clean water barrel and the first pump as well as between the clean water barrel and the second pump.

Still be equipped with filter and attach fitting between lotion bucket and the third pump.

And a filter is arranged between the reagent disk and the fifth valve.

Further, the incubation unit includes an incubation rack provided with a plurality of spaces for receiving the slats (cards), a thermostat for heating a problem in the incubation rack, and an incubation sensor for constantly detecting an ambient temperature in the incubation rack, and controls movement of the incubation rack by an incubation rack motor and an incubation rack guide rail to correspond to a horizontal position of the slat (card) pushed by the push bar.

Further, the detecting element is including detecting the cassette, detecting the cassette support, sets up the detector that detects the cassette top, it passes through motor, belt and drive wheel to detect the cassette support and removes along the guide rail, and the push rod satisfies lath (card) propelling movement of incubation time with the incubation unit to detecting in the cassette, detects the cassette support and drives and detect the cassette and remove and detect under the detector, and the back push rod that finishes detecting pushes away lath (card) to the waste hole, and the waste hole links to each other with waste recovery unit.

The utility model provides a full-automatic immunodetection method of flowing water, includes to be surveyed district, bar code recognition district, transfer area, a plurality of detection regions, puts in order district, controller and power, the detection region is by advancing kind/retrieve unit, conveyer belt unit, shake even unit, lath storehouse unit, reaction cup transfer unit, sample arm, reagent storehouse unit, incubation unit, liquid path unit, waste recovery unit, detection unit and constitute in the system through following step realization:

step 1, conveying a sample in a to-be-detected area through a conveying area, scanning a code to obtain the information of the current to-be-detected sample during conveying, placing a test tube where the sample is located on a sample support, and sticking a corresponding bar code identifier on a test tube arm;

step 2, obtaining an empty reaction cup from the reaction cup transfer unit;

step 3, transmitting the current sample to be detected to a sample introduction/recovery unit of a corresponding detection area according to the obtained information of the sample to be detected, preparing a corresponding batten by a batten stacking unit, and preparing a corresponding reagent by a reagent bin unit;

step 4, the sample to be detected enters a detection area, the shaking unit sequentially grabs the sample to be detected on the support, shakes uniformly, and then sequentially puts back the support;

step 5, the reagent arm extracts prepared reagent from the reagent bin unit through the reagent needle and injects the reagent into the reaction cup, and the reagent needle on the reagent arm cleans the needle and the needle body through the liquid path unit after the reagent is completed;

step 6, the sample arm extracts the uniformly shaken sample to be detected through the sample needle, injects the sample to be detected into the reaction cup and stirs the sample, and sends the sample to be detected to a placing area through the sample introduction/recovery unit after the sample to be detected on the bracket is finished;

step 7, extracting the mixed solution after stirring by the sample arm through the sample needle, adding the mixed solution onto a prepared lath, cleaning the needle interior and the needle body by the sample needle through the liquid path unit after the operation is finished, extracting the residual solution in the reaction cup by the reagent arm through the waste liquid needle, pushing the empty reaction cup to the waste material recovery unit through the waste liquid needle, and cleaning the waste liquid needle through the liquid path unit by the waste liquid needle after the operation is finished;

step 8, feeding the lath added with the mixed solution into an incubation unit and setting incubation time;

9, sending the laths meeting the incubation time into a detection unit for detection, and discarding the laths through waste material holes in the detection unit after the detection is finished;

step 10 obtains the final detection information.

By adopting the technical scheme of the utility model, the utility model has the beneficial effects that: the method is suitable for multiple sample types, multiple indexes, multiple threads and pipelined asynchronous execution detection, greatly improves the detection speed and application flexibility, can adapt to the application requirements of more clients, reduces the detection waiting time and further increases the use convenience.

Drawings

FIG. 1 is a schematic diagram of a detection region in a full-automatic flow immunoassay system according to the present invention;

FIG. 2 is a schematic diagram of a shake-up unit structure provided by the present invention;

FIG. 3 is a schematic structural view of a transfer unit of the reaction cup provided by the present invention;

FIG. 4 is a schematic diagram of a sample arm configuration provided by the present invention;

FIG. 5 is a schematic diagram of the structure of the reagent arm and a part of the transfer unit of the reaction cup provided by the present invention;

FIG. 6 is a schematic diagram of a reagent cartridge unit according to the present invention;

FIG. 7 is a schematic view of a fluid path structure of a detection area provided by the present invention;

FIG. 8 is a schematic view of a slat stack unit according to the present invention;

FIG. 9 is a schematic view of the structure of an incubation unit provided by the present invention;

FIG. 10 is a schematic structural diagram of a detecting unit provided by the present invention;

FIG. 11 is a schematic structural diagram of a flow-water fully-automatic immunoassay system provided by the present invention.

Wherein, 1, equipment support, 2, shake even unit, 3, reaction cup transfer unit, 4, sample arm, 5, reagent arm, 6, reagent storehouse unit, 7, liquid path unit, 8, lath stack unit, 9, incubation unit, 10, detecting element, 11, advance kind/recovery unit, 12, conveyer belt unit, 13, detection area, 14, put in order the district, 15, wait to examine the district, 16, bar code identification district, 17, transfer area

201. A test tube clamp 202, an anti-drop strip 203, a shaking motor 204, a Z-axis motor 205, a Y-axis motor,

301. reaction cup holder 302, hand grip 303, reaction cup holder 304, spring 305, X-direction motor 306, Z-direction motor 307, movable cup holder 308, reaction cup holder guide rail 309, drawer 310, hand grip support 311, movable support,

401. a sample needle 402, an anti-collision device 403, a stirring device 404, a direct current motor 405, a needle washing pool 406, a code disc 407, an arm group rotating motor 408, an arm group lifting motor 409, an arm group motor bracket 410, a movable rod 411 and a sample needle seat,

501. a reagent needle 502, a waste liquid needle 503, a double needle motor 504, a double needle motor bracket 505, a guide rail motor 506, a reagent needle pump 507, a waste liquid needle pump 508, a double needle bracket 509, a guide rail bracket,

601. a heat radiation fan, 602, a heat radiation fin, 603, a reagent bottle, 604, a reagent tray, 605, a bearing, 606, a bin body, 607, heat preservation cotton, 608, a window, 609, a bar code machine, 610, a sensor, 611, a refrigeration sheet, 612, an air guide device,

701. a first pump 702, a first valve 703, a first plunger pump 704, a second pump 705, a second valve 706, a second plunger pump 707, a third pump 708, a third valve 709, a reagent needle port 710, a fourth pump 711, a fourth valve 712, a fifth pump 713, a fifth valve 714, a sixth pump 715, a sample needle port 716, a reagent disk port 717, a waste needle port 718, a connector 719, a filter 720, a clean water bucket 721, a wash water bucket 722, a waste water bucket,

801. a card frame 802, a card taking arm 803, a card taking hook 804, a card taking telescopic rod 805, a card seat 806, a card taking arm support 807, a push rod 808, a card frame code scanner 809, a card frame code scanner support,

901. incubation rack, 902, thermostat, 903, incubation sensor, 904, incubation rack motor, 905 incubation rack guide, 1001, detection cartridge, 1002, detection cartridge holder, 1003, detector, 1004, waste well.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

A running water full-automatic immunity detection system comprises a to-be-detected area 15, a bar code identification area 16, a conveying area 17, a plurality of detection areas 13, a homing area 14, a controller and a power supply, wherein each detection area 13 consists of a sample introduction/recovery unit 11, a conveying belt unit 12, a shaking unit 2, a batten stacking unit 8, a reaction cup transfer unit 3, a sample arm 4, a reagent arm 5, a reagent bin unit 6, an incubation unit 9, a liquid path unit 7, a waste recovery unit and a detection unit 10;

a plurality of samples to be detected are placed in the area 15 to be detected, the samples to be detected are placed in a test tube on a test tube support, an identification bar code corresponding to the information of the current samples to be detected is arranged on the test tube, the area 15 to be detected is connected with a sample introduction/recovery unit 11 of a detection area 13, a reset area 15 and the detection area 13 through a transmission area 17, one side of the sample introduction/recovery unit 11 is connected with the transmission area 17 and used for transmitting the samples to be detected and the detected samples between the transmission area 17 and the detection area 13, the other side of the sample introduction/recovery unit 11 is provided with a conveyor belt unit 12 and used for transmitting the samples to be detected to the shaking unit 2 one by one, a bar code identification area 16 is arranged on a channel of the transmission area 17, the conveyor belt unit 12 is adjacent to the shaking unit 2, a sample arm 4 is arranged above the shaking unit 2, a batten stack unit 8 is arranged on one side of the sample arm 4, the strip stacking unit 8 is provided with a strip conveying mechanism, a reaction cup transferring unit 3 is arranged above the strip stacking unit 8, the reaction cup transferring unit 3 is provided with a reaction cup conveying device, the reaction cup conveying device is adjacent to the reagent arm 5, and a waste material recycling unit is arranged below the reaction cup transferring unit 3; reagent arm 5 one side is equipped with reagent storehouse unit 6, be connected with liquid way unit 7 on the reagent arm 5, reagent arm 5 opposite side is equipped with incubation unit 9, incubation unit 9 one side is equipped with detecting element 10, be equipped with push mechanism between lath stack unit 8, incubation unit 9 and the detecting element 10, push mechanism is the push rod, through push rod's removal, with the lath from lath stack unit 8 propelling movement to incubation unit 9 and detecting element 10, sample arm 4 and reagent arm 5 link to each other with liquid way unit 7.

Sensors and push rods are arranged between the conveying area 17 and the arrangement area 14 and between the sample introduction/recovery unit 11, the sensors are used for detecting whether a current sample bearing support is in place or not, the push rods are started after the current sample bearing support is in place, the support is pushed to enter the sample introduction unit, pull pieces are arranged on two sides of a channel in the area to be detected 15, the arrangement area 14 and the sample introduction/recovery unit 11 respectively, the pull pieces are telescopic movable pull pieces, each pull piece is provided with an electronic switch, and after the pull pieces are started to push and pull the sample support in the stroke movement, the pull pieces are completely unfolded along guide rails in the two sides, and the sample support is pushed and pulled to a designated position, and then the pull pieces retract to the two sides of the channel.

Shake even unit 2 and include test tube clamp 201, anticreep strip 202, shake even motor 203, Z axle motor 204, Y axle motor 205, test tube clamp 201 top is equipped with anticreep strip 202, prevents to shake even in-process test tube clamp 201 in and drops, shake even motor 203 and test tube clamp 201 swing joint, test tube clamp 201 shake even activity along with shaking even motor 203's rotation, Z axle motor 204 is through belt and runner on the Z axle motor support and shake even motor 203 swing joint, Y axle motor 205 passes through belt and runner and Z axle motor 204 swing joint on the Y axle motor support, makes test tube clamp 201 around can, reciprocates, realizes mentioning the test tube from the support, the action of putting down.

The batten stacking unit 8 comprises a clamping frame 801, a clamping taking arm 802, a push rod 807, a clamping frame bar scanner 808 and a clamping frame bar scanner support 809, wherein battens (clamps) are placed in the clamping frame 801, battens corresponding to different detection items are placed in different clamping frames 801, the clamping frame bar scanner 808 is arranged on the rear side of the clamping frame 801 and used for distinguishing items tested by the battens in the clamping frame and the number of the battens, the clamping frame bar scanner 808 is arranged on the clamping frame bar scanner support 809, a clamping taking arm support 806 is arranged below the clamping frame bar scanner support 809, the push rod 807 and the clamping taking arm 802 are arranged on the clamping taking arm support 806, a motor is arranged below the clamping taking arm 802 and can enable the clamping taking arm 802 to rotate, a clamping frame 805 is arranged on the clamping taking arm 802, a clamping taking telescopic rod 804 is arranged below the clamping frame, a taking hook 803 is arranged at one end of the clamping taking telescopic rod 804, the taking hook 803 is a telescopic activity, and when the battens (clamps) are taken, the clamping hook telescopic rod 804 extends forwards along a guide rail at the bottom of the clamping frame, the hook 803 is pushed against the bottom of the clamp frame and extends forwards, the bottom of the clamp frame 801 is provided with a hook taking groove, when the hook 803 reaches the hook taking groove opening at the bottom of the clamp frame 801, the hook smoothly slides into the groove to hook the batten (hook) at the bottom of the clamp frame 801, and the height of the hook is consistent with the thickness of the batten (hook).

The reaction cup transfer unit 3 comprises a drawer 309, reaction cup holders 301, a hand grip 302, a reaction cup holder 303, a movable cup holder 307, a hand grip moving unit and a reaction cup holder guide rail 308, wherein the one or more reaction cup holders 301 are arranged in the drawer 309, empty reaction cups are stored in the reaction cup holders 301, the hand grip 302 is arranged above the reaction cup holders 301, the hand grip 302 is connected with the hand grip moving unit through a hand grip support 310, the reaction cup holder 303 is connected with the reaction cup holder guide rail 308 through the movable cup holder 307, the hand grip moving unit comprises a hand grip support 310 and a movable support 311, the movable support 311 controls the movable support 311 to move back and forth through a motor, the hand grip support 310 is arranged on the movable support 311 and movably connected with an X-direction motor 305 through a belt to control the left and right movement of the hand grip 302, and the Z-direction motor 306 is arranged on the support 310, the Z-direction motor 306 is movably connected with the hand grip 302 through a belt and a transmission wheel to control the hand grip 302 to move up and down, the guide rail is movably connected with the movable cup holder 307, the movable cup holder 307 can move along the guide rail, a reaction cup holder 303 is arranged on the movable cup holder 307, a reset spring 304 is arranged on the reaction cup holder 303, the hand grip 302 is provided with an electromagnetic valve and a gripper, the reaction cup guide rail 308 is arranged on a guide rail support, the guide rail support is movably connected with the reagent arm 5 through the motor, the belt and the transmission wheel, a double-needle support 508 is arranged on the reagent arm 5, a reagent needle 501 and a waste liquid needle 502 are arranged on the double-needle support 508, and the reagent needle 501 and the waste liquid needle 502 are connected with a liquid path unit 7.

The sample arm 4 comprises a sample needle 401, a stirring device 403, a movable rod 410, an arm group motor bracket 409, an arm group rotating motor 407 and an arm group lifting motor 408, the sample needle 401 is arranged on a sample needle holder 411, a movable rod 410 is connected with the sample needle holder 411, the movable rod 410 is provided with a stirring device 403, the movable rod 410 is connected with an arm group motor bracket 409, the arm group motor bracket 409 is provided with an arm group rotating motor 407 and an arm group lifting motor 408, the sample needle holder 411 is provided with an anti-collision device 402, the anti-collision device 402 is arranged on the top of the sample needle 401, a buffer space is artificially arranged, the hard injury caused by the contact of the needle head when the sample needle 401 moves up and down is prevented, the stirring device 403 is provided with a direct current motor 404, the direct current motor 404 is connected with an eccentric plate, a sample needle through hole is formed in the eccentric plate, and a needle washing pool is arranged below the sample needle through hole. The sample needle 401 is lifted through the sample needle through hole, the through hole is arranged on the eccentric plate, and when the DC motor 404 drives the eccentric plate, the sample needle through hole surrounding the sample needle 401 rotates, and simultaneously drives the sample needle 401 to stir; and a needle washing pool below the sample needle 401 is connected with a washing liquid and can wash the outer wall of the sample needle, and an arm group rotating motor 407 and an arm group lifting motor 408 on the arm group motor support are connected with the movable rod through a driving wheel and a belt. The lifting and the rotation of the movable rod are realized through the positive and negative rotation of the motor, the lifting and the rotation of the sample needle seat are driven at the same time, a code disc 406 is arranged between the movable rod 410 and the arm group rotating motor 407, and the rotation degree of the sample needle rocker arm is calibrated through the code disc 406.

The reagent bin unit 6 comprises a heat radiation fan 601, a heat radiation fin 602, reagent bottles 603, a 606 bin body, a heat preservation layer, a window 608, a bar code machine 609, a sensor 610, a refrigerating sheet 611 and an air guide device 612, wherein the plurality of reagent bottles 603 are distributed in the reagent tray 604 in a fan shape, the bottom of the reagent tray 604 is connected with a bearing, the bearing is connected with a stepping motor and a stepping sensor, the motor is connected with the bearing and can drive the whole bin body 606 to rotate, a notch corresponding to a bar code on the reagent bottle 603 is arranged on the reagent tray 604, the bin body 606 is provided with a window corresponding to the notch, one side of the window is provided with the bar code machine 609, the bin body 606 is rotated, the bar code machine 609 can read information of each reagent bottle 603 through the rotation of the bin body 606, the heat radiation fin 602 is arranged in the center of the reagent tray 604, the heat radiation fan 601 is arranged above the heat radiation fin 602, and the refrigerating sheet 611 is arranged below the bin body 606, the refrigerating sheet 611 is connected with an air guide device 612, a stepping motor and a stepping sensor are connected below the reagent tray 604, the motor connecting bearing can drive the whole bin body 606 to rotate, a heat insulation layer is arranged outside the bin body 606 and is made of heat insulation cotton 607, and the bottom of the bin body 606 is connected with the liquid path unit 7 and used for discharging condensed water generated during refrigeration.

The liquid path unit 7 comprises a reagent needle internal and external cleaning liquid path, a sample needle internal clear water cleaning liquid path, a sample needle external cleaning liquid path and a waste liquid discharge liquid path;

the internal and external cleaning liquid path of the reagent needle 501 comprises a cleaning water barrel 720, a first pump 701, a first valve 702 and a first needle washing pool which are sequentially communicated, the reagent needle 501 is arranged in the first needle washing pool, one path of the first valve 702 and the first plunger pump 703 are connected with the inner wall of the reagent needle 502 through a reagent needle interface 709 for cleaning the reagent needle, the other path of the first valve 702 is connected with the first needle washing pool for cleaning the outer wall of the reagent needle 501, and the first plunger pump 703 can draw and discharge the reagent solution through the reagent needle 501;

the clean water cleaning liquid path on the inner side of the sample needle 401 comprises a clean water barrel 720, a second pump 704, a second valve 705, a second plunger pump 706 and a fourth valve 711 which are sequentially communicated, the second plunger pump 706 is connected with the interior of the sample needle 401 through a sample needle interface 715, when the fourth valve 711 is gated, the clean water cleaning liquid path on the inner side of the sample needle 401 cleans the inner wall of the sample needle 401, and the second plunger pump 706 can extract and discharge a sample solution through the sample needle;

the washing liquid cleaning liquid path on the outer side of the sample needle 401 comprises a washing liquid barrel 721, a third pump 707 and a third valve 708 which are sequentially communicated, the sample needle 401 is arranged in the second needle washing pool, the third valve 708 is connected with the outer wall of the second needle washing pool, and when the third valve 708 is gated, the washing liquid cleaning liquid path on the outer side of the sample needle 401 cleans the outer wall of the sample needle 401.

The liquid path system further comprises a condensate and waste liquid discharge path, the condensate and waste liquid discharge path comprises a waste liquid needle 502, a fifth pump 712 and a fifth valve 713, the waste liquid needle 502, the fifth pump 712 and the fifth valve 713 are sequentially communicated, one path of the fifth valve 713 is connected with the reagent disk through a reagent disk interface 716, the other path of the fifth valve is connected with the waste liquid needle 502 through a waste liquid needle interface 717, and the fifth pump 712 is used for discharging the condensate in the reagent disk and the waste liquid sucked by the waste liquid needle 502 into a waste liquid barrel 722.

The bottom of the first needle washing pool is connected with a fourth pump 710, and the fourth pump 710 is used for discharging the waste liquid of the first needle washing pool to a waste liquid barrel 722.

The bottom of the second needle washing pool is connected with a sixth pump 715, and the sixth pump 715 is used for discharging the waste liquid in the second needle washing pool to the waste liquid barrel 722.

A filter 719 and a connecting joint 718 are further arranged between the clean water tank 720 and the first pump 701 and the second pump 704.

A filter and a connecting joint are also arranged between the washing liquid barrel 721 and the third pump 707.

A filter is provided between the reagent disk and the fifth valve 713.

The incubation unit 9 comprises an incubation rack 901, a thermostat 902 and an incubation sensor 903, wherein the incubation rack 901 is provided with a plurality of spaces for placing the laths (cards), the thermostat 902 is used for heating the problems in the incubation rack 901, the incubation sensor 903 detects the ambient temperature in the incubation rack 901 at any time, and the incubation unit 9 controls the movement of the incubation rack 901 through an incubation rack motor 904 and an incubation rack guide 905 so as to correspond to the horizontal positions of the laths (cards) pushed by a push rod 807.

The detection unit 10 comprises a detection clamping seat 1001, a detection clamping seat support 1002 and a detector 1003 arranged above the detection clamping seat 1001, the detection clamping seat support 1002 moves along a guide rail through a motor, a belt and a driving wheel, a push rod 807 pushes a batten (card) of which the incubation time is met by the incubation unit 9 into the detection clamping seat 1001, the detection clamping seat support 1002 drives the detection clamping seat 1001 to move to the position below the detector 1003 for detection, the push rod 807 pushes the batten (card) to a waste hole 1004 after the detection is finished, and the waste hole 1004 is connected with a waste recovery unit.

The running water full-automatic immunodetection system and the detection method thereof can be used for measuring various detection items, such as: c-reactive protein assay, neutrophil gelatinase-associated lipocalin assay, beta-human chorionic gonadotropin assay, D-dimer assay, procalcitonin assay, heparin-binding protein assay, whole course C-reactive protein assay, troponin I assay, creatine kinase isozyme assay, amino-terminal pro-brain peptide assay, progesterone assay, serum amyloid A assay, cardiac fatty acid-binding protein assay, troponin I assay, B-type natriuretic peptide assay, myoglobin assay, soluble growth stimulation expression gene 2 protein assay, neutrophil gelatinase-associated lipocalin assay, lipoprotein-associated phospholipase A2 assay, urinary microalbumin assay, antimuilerian hormone assay, beta 2 microsphere assay, interleukin 6 assay, pepsin 1/2 assay, etc., adjusted accordingly to parameters such as shaking speed and incubation reaction time according to specific items, all can be realized by the technology of the utility model.

The running water full-automatic immunoassay method comprises a to-be-detected area 15, a bar code identification area 16, a conveying area 17, a plurality of detection areas 13, a homing area 14, a controller and a power supply, wherein the detection areas 13 are formed by a sample introduction/recovery unit 11, a conveying belt unit 12, a shaking unit 2, a batten stack unit 8, a reaction cup transfer unit 3, a sample arm 4, a reagent arm 5, a reagent bin unit 6, an incubation unit 9, a liquid path unit 7, a waste recovery unit and a detection unit 10, and the method is realized through the following steps:

step 1, conveying a sample in a to-be-detected area through a conveying area 17, obtaining current to-be-detected sample information through a bar code identification area 16 during conveying, placing a test tube where the sample is located on a sample support, and sticking a corresponding bar code identifier on a test tube arm;

step 2, obtaining an empty reaction cup from the reaction cup transfer unit 3;

step 3, the current sample to be detected is transmitted to a sample introduction/recovery unit 11 corresponding to the detection areas 13 according to the obtained information of the sample to be detected, because the detection items are various, blank slats corresponding to different detections are stored in different detection areas, meanwhile, the detection areas detect the remaining quantity of the slats, when the remaining items detect that the blank slats are insufficient, the system automatically distributes the current sample to be detected to the detection areas 13 corresponding to the blank slats, a slat stacking unit 8 prepares corresponding slats, and a reagent cabin unit 6 prepares corresponding reagents;

step 4, the sample to be detected enters an appointed detection area 13, the shaking unit 2 sequentially picks the sample to be detected on the support, shakes the sample to be detected evenly, and then sequentially puts the sample to be detected back to the support after shaking the sample to be detected evenly;

step 5, the reagent arm 5 extracts prepared reagent from the reagent bin unit 6 through the reagent needle and injects the reagent into the reaction cup, and the reagent needle on the reagent arm 5 cleans the needle and the needle body through the liquid path unit 7 after the reagent is completed;

step 6, the sample arm 4 extracts the uniformly shaken sample to be detected through the sample needle, injects the sample to be detected into the reaction cup and stirs the sample, and sends the sample to be detected to the arrangement area 14 through the sample introduction/recovery unit 11 after the sample to be detected on the bracket is finished;

step 7, a sample arm 4 extracts the mixed solution after stirring through a sample needle and adds the mixed solution to a prepared lath, the sample needle cleans the needle body and the needle body through a liquid path unit 7 after the operation, a reagent arm 5 extracts the residual solution in the reaction cup through the waste liquid needle and pushes the empty reaction cup to a waste material recovery unit through the waste liquid needle, and the waste liquid needle cleans the waste liquid needle through the liquid path unit 7 after the operation is finished;

step 8, feeding the lath added with the mixed solution into an incubation unit 9 while setting the incubation time;

step 9, sending the laths meeting the incubation time into a detection unit 10 for detection, and discarding the laths through waste holes 1004 on the detection unit after the detection is finished;

step 10 obtains the final detection information.

It is to be noted that the foregoing is only illustrative of the preferred embodiments of the present invention and the technical principles employed. Those skilled in the art will appreciate that the present invention is not limited to the particular embodiments described herein, and that various obvious changes, rearrangements and substitutions will now be apparent to those skilled in the art without departing from the scope of the utility model. Therefore, although the present invention has been described in some detail by the above embodiments, the utility model is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the utility model, and the scope of the utility model is determined by the scope of the appended claims.

Claims (8)

1. A running water full-automatic immunity detection system is characterized by comprising a to-be-detected area, a bar code identification area, a conveying area, a plurality of detection areas, a placing area, a controller and a power supply, wherein each detection area consists of a sample introduction/recovery unit, a conveying belt unit, a shaking unit, a batten stacking unit, a reaction cup transfer unit, a sample arm, a reagent bin unit, an incubation unit, a liquid path unit, a waste recovery unit and a detection unit;

the area to be detected is connected with the detection area and the homing area through the transmission area, the transmission area is provided with a bar code identification area, the detection area is provided with a sample introduction/recovery unit, one side of the sample introduction/recovery unit is connected with the transmission area, the other side of the sample introduction/recovery unit is provided with a transmission belt unit, the conveyor belt unit is adjacent to the shaking unit, a sample arm is arranged above the shaking unit, a batten stacking unit is arranged on one side of the sample arm, a reaction cup transfer unit is arranged above the batten stacking unit and is adjacent to the reagent arm, a waste recovery unit is arranged below the reaction cup transfer unit, a reagent bin unit is arranged on one side of the reagent arm, the reagent arm other side is equipped with the incubation unit, incubation unit one side is equipped with the detecting element, be equipped with push mechanism between lath stack unit, incubation unit and the detecting element, sample arm and reagent arm link to each other with the liquid way unit.

2. The running water full-automatic immunodetection system of claim 1, wherein a sensor and a push rod are arranged between the conveying area and the homing area and between the conveying area and the sample introduction/recovery unit, and pulling pieces are arranged on two sides of a channel in the area to be detected, the homing area and the sample introduction/recovery unit, and are retractable movable pulling pieces.

3. The running water full-automatic immunodetection system of claim 1, wherein the shake-up unit comprises a test tube clamp, an anti-drop strip, a shake-up motor, a Z-axis motor and a Y-axis motor, the anti-drop strip is arranged above the test tube clamp, the shake-up motor is movably connected with the test tube clamp, the Z-axis motor is movably connected with the shake-up motor through a Z-axis motor support, and the Y-axis motor is movably connected with the Z-axis motor through a Y-axis motor support.

4. The flow-line full-automatic immunoassay detection system of claim 1, wherein the slat stacking unit comprises a rack, a card-taking arm, a push rod, a rack bar scanner and a rack bar scanner support, the slat is placed in the rack, the rack bar scanner is arranged on the rear side of the rack, the rack bar scanner is arranged on the rack bar scanner support, the card-taking arm support is arranged below the rack bar scanner support, the push rod and the card-taking arm are arranged on the card-taking arm support, a card holder is arranged on the card-taking arm, a card-taking telescopic rod is arranged below the card holder, and a card-taking hook is arranged at one end of the card-taking telescopic rod.

5. The running water full-automatic immunity detection system of claim 1, wherein the reaction cup transfer unit comprises a drawer, reaction cup holders, a gripper, a reaction cup holder, a movable cup holder, a gripper moving unit and a reaction cup holder guide rail, one and/or more reaction cup holders are arranged in the drawer, the gripper is arranged above the reaction cup holders, the gripper is connected with the gripper moving unit through a gripper support, the reaction cup holders are connected with the reaction cup holder guide rail through the movable cup holders, the gripper moving unit comprises a gripper support and a movable support, the gripper support is arranged on the movable support and movably connected with an X-direction motor, a Z-direction motor is arranged on the gripper support and movably connected with the gripper, the guide rail is movably connected with the movable cup holders, the reaction cup holders are arranged on the movable cup holders, the reaction cup holders are provided with return springs, the reagent bottle opener is characterized in that the gripper is provided with an electromagnetic valve and a gripper, a reaction cup guide rail is arranged on a guide rail support, the guide rail support is movably connected with the reagent arm, the reagent arm is provided with a double-needle support, the double-needle support is provided with a reagent needle and a waste liquid needle, and the reagent needle and the waste liquid needle are connected with a liquid path unit.

6. The flow full-automatic immunoassay system of claim 1, wherein the sample arm comprises a sample needle, a stirring device, a movable rod, a motor bracket, an arm group rotating motor and an arm group lifting motor, the sample needle is arranged on a sample needle seat, the sample needle seat is connected with the movable rod, the movable rod is provided with the stirring device, the movable rod is connected with the motor bracket, the motor bracket is provided with the arm group rotating motor and the arm group lifting motor, the sample needle seat is provided with an anti-collision device, the stirring device is provided with a direct current motor, the direct current motor is connected with an eccentric plate, the eccentric plate is provided with a sample needle through hole, a needle washing pool is arranged below the sample needle through hole, the arm group rotating motor and the arm group lifting motor on the motor bracket are connected with the movable rod through a driving wheel and a belt, a code disc is arranged between the movable rod and the arm group rotating motor, the sample arm is connected with a liquid path unit.

7. The running water full-automatic immunoassay system of claim 1, wherein the reagent chamber unit comprises a heat radiation fan, heat radiation fins, reagent bottles, a chamber body, a heat insulation layer, a window, a bar code machine, a sensor, a cooling fin and an air guide device, the reagent bottles are distributed in a fan shape and are arranged in a reagent tray, the bottom of the reagent tray is connected with a bearing, the reagent tray is provided with a notch corresponding to a bar code of the reagent bottles, the chamber body is provided with the window corresponding to the notch, one side of the window is provided with the bar code machine, the center of the reagent tray is provided with the heat radiation fins, the cooling fin is arranged above the heat radiation fins, the cooling fin is arranged below the chamber body and is connected with the air guide device, the stepping motor and the stepping sensor are connected below the reagent tray, and the heat insulation layer is arranged outside the chamber body.

8. The flow-through fully-automatic immunoassay system of claim 1, wherein the fluid path unit comprises a reagent needle internal and external wash fluid path, a sample needle internal clean water wash fluid path, a sample needle external wash fluid path, and a waste fluid discharge fluid path.

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