CN217022316U - Novel assembly line type mobile nucleic acid detection vehicle - Google Patents

Abstract

The utility model discloses a novel assembly line type mobile nucleic acid detection vehicle, which comprises a vehicle body, a cab, a detection chamber, an inlet, a buffer room, a workbench, a biological safety cabinet, a full-automatic nucleic acid extraction and fluorescence PCR system, an autoclave, a refrigerator and a storage cabinet, wherein the interior of the vehicle body is divided into the cab and the detection chamber; the middle part of the vehicle body is provided with an inlet and a buffer room for entering the detection chamber; a workbench is arranged opposite to the buffer room; a biological safety cabinet is arranged opposite to the workbench, and a full-automatic nucleic acid extraction and fluorescence PCR system is arranged on the right side of the workbench; the refrigerator and the storage cabinet are arranged opposite to the full-automatic nucleic acid extraction and fluorescence PCR system, and the whole vehicle is provided with a negative pressure ventilation system and an autoclave. The utility model can realize mobile detection and the requirements of quick, accurate and efficient full-automatic nucleic acid detection under the condition of meeting the requirement of biological safety; the detection moving vehicle can be driven to a place needing detection, so that the detection can be carried out immediately, the sample transfer time is shortened, and the requirement of emergency detection is met.

Description

Novel assembly line type mobile nucleic acid detection vehicle

Technical Field

The utility model belongs to the technical field of nucleic acid detection equipment, and particularly relates to a novel assembly line type mobile nucleic acid detection vehicle.

Background

After the new coronavirus pneumonia epidemic situation occurs, when the aim of zero clearing is realized in the current country, the sporadic epidemic situation occurs, the rapid response capability of public health departments all over the country is examined, and the time of the whole process is required to be shortened as much as possible for tracking the nucleic acid detection results of close-cut splicers and sub-close splicers more quickly. The existing nucleic acid detection laboratory or mobile nucleic acid detection vehicle has the following defects: 1. the nucleic acid detection laboratory arranged by a detection unit is relatively fixed in place and cannot move, the temporarily built nucleic acid detection laboratory has a large biological safety risk, a long time is required for transporting a sample to the laboratory, and a risk of biological hazard exists in the transportation process; 2. the improvement and speed improvement of detection reagents and detection equipment have limitations and cannot be greatly shortened in time due to the limitation of objective conditions; 3. at present, the domestic nucleic acid detection vehicle mainly adopts the modes of steps such as sample inactivation, nucleic acid extraction, gene amplification and the like, certain connection time exists among the steps, unnecessary pollution is easily caused by improper operation, and the mobile detection vehicle has small area, so that the contained equipment is limited, and the sample can not be detected immediately. At this time, on the premise of meeting biological safety requirements, a novel mobile production line type full-automatic nucleic acid detection vehicle which is mobile, can detect nearby and can detect a single sample immediately needs to be designed.

SUMMERY OF THE UTILITY MODEL

Aiming at the defects of the prior art, the utility model provides a novel assembly line type mobile nucleic acid detection vehicle.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a novel assembly line type mobile nucleic acid detection vehicle comprises a vehicle body, a cab, a detection chamber, an inlet, a buffer chamber, a workbench, a biological safety cabinet, a full-automatic nucleic acid extraction and fluorescence PCR system, a refrigerator and a storage cabinet, wherein the interior of the vehicle body is divided into the cab and the detection chamber; an inlet for entering the detection chamber is formed in the middle of the vehicle body, and a buffer room is arranged in the inlet; a workbench is arranged opposite to the outlet of the buffer room; a biological safety cabinet is arranged opposite to the workbench, and a full-automatic nucleic acid extraction and fluorescence PCR system is arranged on the right side of the workbench; the refrigerator and the storage cabinet are arranged side by side opposite to the full-automatic nucleic acid extraction and fluorescence PCR system.

The sample sampling device comprises a worktable, a sampling tube and a centrifugal machine, wherein the worktable is fixedly provided with a centrifugal machine and a vibration mixer on the left side, the centrifugal machine is used for carrying out centrifugal treatment on a sample to be detected, and the vibration mixer is used for carrying out vibration mixing on the sample to ensure the uniformity and stability of the sample in the sampling tube; the centrifuge and the oscillation blending machine are fixed on the left side of the workbench, so that normal operation of workers is not obstructed, and the workers can conveniently move to operate at any time.

The refrigerator is characterized by further comprising a negative pressure device and a turbofan, wherein the negative pressure device is respectively arranged in the workbench and beside the refrigerator, and the negative pressure device pumps and exhausts air in the detection chamber through the turbofan arranged at the top of the refrigerator body, so that a negative pressure space is formed in the detection chamber; the negative pressure device has the function of ensuring that a working area in the vehicle is a negative pressure area, an environment area outside the vehicle is a positive pressure area, and air in the laboratory flows from the clean area to the polluted area, so that the pressure in the laboratory is lower than the outdoor pressure. A negative pressure device is loaded in the diagonal area of the detection chamber, so that the negative pressure setting of the whole area is met.

The consumable cabinet and the high-pressure sterilizer are arranged at the left end of the detection chamber, the consumable cabinet is close to the workbench, and the consumable is convenient to take out after the staff turns; the high-pressure sterilizer arranged at the rear part of the vehicle is used for sterilizing the sewage, so that aerosol or virus generated in the experiment can not be leaked to pollute the external environment, and medical waste generated in the experiment activity can not pollute the outdoor environment after being sterilized under high pressure.

Furthermore, the detection chamber is a closed space, and the detection chamber and the outside air exchange are required to be disinfected and sterilized.

Furthermore, a folding chair is arranged in the cab, and a seat is provided for people except the driver.

The utility model has the advantages that: 1. under the condition of meeting the requirement of biological safety, the mobile detection can be realized, and the requirements of quick, accurate and efficient full-automatic nucleic acid detection can also be realized; 2. the limited space in the bus is fully utilized, and the humanized space setting is adopted, so that the biological safety accidents are effectively prevented, and a good nucleic acid detection environment is provided for laboratory detection personnel; 3. the detection moving vehicle can be driven to a place needing detection, so that the detection can be carried out immediately, the sample transfer time is shortened, and the requirement of emergency detection is met.

Drawings

FIG. 1 is a schematic diagram of the internal structure of a novel assembly line type mobile nucleic acid detecting vehicle according to the present invention;

FIG. 2 is a top view of a novel assembly line type mobile nucleic acid detecting vehicle according to the present invention.

Detailed Description

The utility model is described in detail below with reference to the accompanying drawings:

in the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "front", "left", "right", etc. are based on the directions or positional relationships shown in the drawings, or the directions or positional relationships which are usually placed when the products of the present invention are used, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or the element to be referred must have a specific direction, a specific direction configuration and operation, and thus, the present invention is not to be construed as being limited.

As shown in figure 1, a novel assembly line type mobile nucleic acid detection vehicle comprises a vehicle body 1, a cab 2, a detection chamber 3, an inlet 4, a buffer room 5, a workbench 6, a biological safety cabinet 7, a full-automatic nucleic acid extraction and fluorescence PCR system 8, a refrigerator 9, a storage cabinet 10, a centrifuge 11, a negative pressure device 12, a consumable cabinet 13, a folding chair 14, a turbo fan 15, an autoclave 16 and an oscillation mixer 17, wherein the interior of the vehicle body 1 is divided into the cab 2 and the detection chamber 3, the cab 2 and the detection chamber 3 are hermetically divided, and the cab 2 and the detection chamber 3 are independent areas without intercommunication. A folding chair 14 for the examiner to rest is arranged in the cab 2. All be provided with independent air conditioner in driver's cabin 2 and the detection room 3, the air conditioner in the detection room 3 needs to be provided with the filtration degassing unit, mutual pollution when avoiding inside and outside air exchange. The detection chamber 3 is a closed space, and needs to be disinfected and sterilized when the air inside and outside the detection chamber is exchanged, so that the environmental requirements of biological experiments are met. The detection chamber 3 is provided with an inlet 4 into the detection chamber 3 at the middle part of the vehicle body 1, and the inlet 4 is a middle door of the vehicle body 1. A buffer room 5 is arranged in the inlet 4, the buffer room 5 is used as a transition area for entering the detection chamber 3, and the inspection personnel need to take off the protective clothing in the buffer room 5 when entering and exiting. The opposite surface of the outlet of the buffer room 5 is provided with a workbench 6, and the workbench 6 is placed close to the edge of the detection chamber 3 and is close to the left. The left side of the workbench 6 is provided with a centrifuge 11 and an oscillating mixer 17, the bottom of the workbench 6 is also provided with a negative pressure device 12, the negative pressure device 12 has a filtering function, and air in the detection chamber 3 can be filtered and then discharged to avoid aerosol in the indoor air from polluting the outside air. The negative pressure device pumps and exhausts air in the detection chamber through a turbine fan arranged on the top of the vehicle body, so that a negative pressure space is formed in the detection chamber. A biological safety cabinet 7 is arranged opposite to the workbench 6, and mainly sucks air in the cabinet outwards to keep the interior of the cabinet in a negative pressure state and protect workers through vertical air flow; the outside air is filtered by the high-efficiency air filter and then enters the safety cabinet to avoid the pollution of the processed sample; the air in the cabinet is also filtered by the HEPA filter and then discharged to the atmosphere to protect the environment. The area between the worktable 6 and the biosafety cabinet 7 is an aisle. The right side of the workbench 6 is provided with a full-automatic nucleic acid extraction and fluorescence PCR system 8 which are arranged side by side. A refrigerator 9 and a storage cabinet 10 are arranged in parallel opposite to the full-automatic nucleic acid extraction and fluorescence PCR system 8, wherein the refrigerator 9 is mainly used for temporarily storing samples to be detected or finished detection and also used for storing detection reagents needing low-temperature storage; the cabinet 10 is mainly used for storing consumables required by experiments. A negative pressure device 12 is respectively arranged below the working platform 6 and beside the refrigerator 9, the negative pressure device 12 is mainly used for ensuring that a working area in the vehicle is a negative pressure area, an environment area outside the vehicle is a positive pressure area, and air in the laboratory flows from a clean area to a polluted area, so that the pressure in the laboratory is lower than the outdoor pressure. Be provided with consumptive material cabinet 13 and autoclave 16 in the edge of detection room 3 left end, mainly used deposits the consumptive material that uses often or the consumptive material that uses often on the workstation 6 that uses often not, for example personal protective articles, pipettor suction head, eight connecting tube, 1.5mL centrifuging tube etc.. The autoclave 16 was used for autoclaving medical waste from laboratory activities.

The type adopted by the full-automatic nucleic acid extraction and fluorescence PCR system 8 used in the embodiment is AutoMolec 3000, which can integrate nucleic acid extraction, nucleic acid amplification and result reading, reduce manual operation steps, optimize detection time and flow, and the full-automatic nucleic acid detection system can meet the requirements of adding samples at any time and detecting when the samples are ready; the sample introduction and result analysis are completed by a full-automatic nucleic acid detection system, so that the human error is reduced, the time of each link is saved, and the detection time is greatly reduced; a whole set of detection operation can be completed in a limited space part, the occupied space is small, and the space in the detection chamber 3 is fully utilized.

The specific flow of the assembly line type mobile nucleic acid detection vehicle comprises the following steps: the detection personnel can enter the detection room 3 after passing through the buffer room 5, and simultaneously bring the inactivated (thermal inactivation or chemical inactivation) sample into the detection room, and disinfect the outer surface of the sampling tube by using chlorine-containing disinfection solution in the biological safety cabinet 7, and fully vibrate and mix the sample on the workbench 6 by using the vibration mixer 17 after the disinfection is finished, and then stand. Adding reagent and quality control products on a workbench 6 according to the requirements of a detection system, placing the products into detection equipment, finally opening a sampling tube cover in a biological safety cabinet, directly placing the products into the equipment after centrifuging according to the requirements of the detection steps of full-automatic nucleic acid extraction and a fluorescence PCR system, also transferring sampling liquid in the sampling tube into a 5ml test tube, placing the products into the equipment after centrifuging by a centrifuge 11, setting detection programs and sample information, and running experiments. And after the experiment operation is finished, analyzing the data result, and reporting the detection result according to the flow requirement.

Although particular embodiments of the present invention have been described and illustrated in detail, it should be noted that various changes and modifications could be made to the above-described embodiments without departing from the spirit of the utility model and the scope of the appended claims.

Claims (6)

1. A novel assembly line type mobile nucleic acid detection vehicle is characterized by comprising a vehicle body (1), a cab (2), a detection chamber (3), an inlet (4), a buffer room (5), a workbench (6), a biological safety cabinet (7), a full-automatic nucleic acid extraction and fluorescence PCR system (8), a refrigerator (9) and a storage cabinet (10), wherein the interior of the vehicle body (1) is divided into the cab (2) and the detection chamber (3); an inlet (4) entering the detection chamber (3) is formed in the middle of the vehicle body (1), and a buffer room (5) is formed in the inlet (4); a workbench (6) is arranged right opposite to the outlet of the buffer room (5); a biological safety cabinet (7) is arranged opposite to the workbench (6), and a full-automatic nucleic acid extraction and fluorescence PCR system (8) is arranged on the right side of the workbench (6); the refrigerator (9) and the storage cabinet (10) are arranged in parallel opposite to the full-automatic nucleic acid extraction and fluorescence PCR system (8).

2. The novel assembly line type mobile nucleic acid detecting vehicle according to claim 1, further comprising a centrifuge (11) and a shaking mixer (17), wherein the centrifuge (11) and the shaking mixer (17) are fixedly arranged at the left side of the workbench (6).

3. The novel assembly line type mobile nucleic acid detecting vehicle according to claim 2, further comprising a negative pressure device (12) and a turbo fan (15), wherein a negative pressure device (12) is arranged in the workbench (6) and beside the refrigerator (9); the negative pressure device (12) pumps and exhausts air in the detection chamber (3) through a turbine fan (15) arranged at the top of the vehicle body (1).

4. The novel pipelined mobile nucleic acid detection vehicle as claimed in claim 3, further comprising a consumable cabinet (13) and an autoclave (16), wherein the consumable cabinet (13) and the autoclave (16) are disposed at the left end of the detection chamber (3).

5. The novel pipelined mobile nucleic acid detecting vehicle according to claim 4, wherein the detecting chamber (3) is a closed space.

6. The novel pipelined mobile nucleic acid-detecting vehicle as claimed in claim 5, characterized in that a folding chair (14) is provided in the cab (2).

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