CN216947013U - Integrated sample treatment consumable for automatic nucleic acid detection - Google Patents
Integrated sample treatment consumable for automatic nucleic acid detection Download PDFInfo
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- CN216947013U CN216947013U CN202121201483.3U CN202121201483U CN216947013U CN 216947013 U CN216947013 U CN 216947013U CN 202121201483 U CN202121201483 U CN 202121201483U CN 216947013 U CN216947013 U CN 216947013U
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Abstract
The utility model relates to the technical field of nucleic acid detection, and discloses an integrated sample processing consumable for automatic nucleic acid detection, which comprises a pipe body, wherein a cracking cavity, an extraction cavity and an amplification cavity are arranged in the pipe body, communication channels are respectively formed between the cracking cavity and the extraction cavity and between the extraction cavity and the amplification cavity of the pipe body, a valve core is arranged on the communication channels, a gas channel communicated with the extraction cavity is also arranged on the pipe body, and the gas channel is used for ventilating or exhausting gas for a nucleic acid detection all-in-one machine to the extraction cavity so as to drive the valve core to move, so that the opening and closing of the communication channels are controlled. When the nucleic acid is detected, the cracking, extraction and dilution and amplification reaction of the sample are carried out under the condition of sealing in the corresponding cavity, the integrated nucleic acid detection machine controls the opening and closing of the communicating channel by using the valve core, and drives the nucleic acid sample to flow in different cavities by using pressure change, so that the operations of PCR cracking, extraction, amplification, detection and the like are completed, the sample does not need to be transferred among different instruments, and the sample, personnel and the environment are prevented from being polluted.
Description
Technical Field
The utility model relates to the technical field of nucleic acid detection, in particular to an integrated sample processing consumable for automatic nucleic acid detection.
Background
Currently, in the field of molecular biology, many diagnostic tests involving biological reactions are generally performed in laboratories or hospitals by skilled technicians or using complex equipment, and the procedures are complex and time-consuming, resulting in high detection costs.
The most common nucleic acid detection is fluorescence quantitative PCR (polymerase chain reaction), wherein a fluorescent dye or a fluorescent marker is added in the nucleic acid amplification process, an optical device is adopted to detect the intensity of a fluorescent signal, and the fluorescent signal is processed and analyzed to obtain the nucleic acid amplification result. Traditional PCR detection requires more manual operation participation, the automation degree is low, and the PCR amplification and detection process is mostly realized by depending on different instruments, so that a detection sample needs to be transferred among different instruments, and the pollution risk exists on the sample, the environment and operators.
SUMMERY OF THE UTILITY MODEL
The purpose of the utility model is: the integrated sample processing consumable for the automatic nucleic acid detection is provided to solve the problem that pollution exists when a detection sample for the nucleic acid detection is transferred among different instruments in the prior art.
In order to achieve the purpose, the utility model provides an integrated sample processing consumable for automatic nucleic acid detection, which comprises a tube body, wherein a cracking cavity, an extraction cavity and an amplification cavity are sequentially arranged in the tube body from top to bottom, the cracking cavity is used for containing a lysate, the extraction cavity is used for containing a diluent, communication channels are respectively formed between the cracking cavity and the extraction cavity and between the extraction cavity and the amplification cavity of the tube body, valve cores used for controlling the opening and closing of the communication channels are movably arranged on the communication channels, a gas channel communicated with the extraction cavity is further arranged on the tube body, and the gas channel is used for ventilating or exhausting gas for a nucleic acid detection all-in-one machine to the extraction cavity so as to drive a nucleic acid sample to flow.
Preferably, an installation groove perpendicular to the communication channel is arranged on the pipe body, the valve core is assembled in the installation groove in a sliding manner along the axial direction of the installation groove, a valve hole is arranged on the valve core, and the extending direction of the valve hole is perpendicular to the moving direction of the valve core.
Preferably, the valve holes are two in total, and the two valve holes are arranged in parallel and spaced from each other.
Preferably, a filter membrane is also preset in the cracking cavity.
Preferably, said filter membrane is arranged at the inlet of the communication channel at the bottom of said lysis chamber.
Preferably, the tube body comprises a main tube and an amplification reaction tube detachably connected to the bottom of the main tube, the inner cavity of the amplification reaction tube forms the amplification cavity, and the lysis cavity and the extraction cavity are located in the main tube.
Preferably, the outer part of the cracking cavity is provided with a fixed clamping table extending along the circumferential direction of the tube body.
Preferably, the cracking chamber and the valve core are arranged coaxially with the gas channel, and the extraction chamber is arranged offset from a center line of the cracking chamber.
Preferably, a pipe cap is detachably arranged on the top of the pipe body.
Compared with the prior art, the integrated sample processing consumable for automatic nucleic acid detection has the beneficial effects that: the cracking cavity, the extraction cavity and the amplification cavity are all arranged in the tube body, when nucleic acid detection is carried out, the cracking, extraction dilution and amplification reaction of a sample are carried out under the closed condition in the corresponding cavity, when nucleic acid detection is carried out, the corresponding valve core is opened, the nucleic acid detection all-in-one machine utilizes the gas channel to ventilate and exhaust the extraction cavity, as the extraction cavity is communicated with the communication channel, the change of the pressure in the extraction cavity drives the nucleic acid sample to flow in different cavities, the multi-step operations of PCR cracking, extraction, amplification, detection and the like can be completed, the sample does not need to be transferred among different instruments, and the sample, personnel and the environment are prevented from being polluted.
Drawings
FIG. 1 is a schematic structural diagram of an integrated sample processing consumable for automated nucleic acid detection according to the present invention;
FIG. 2 is a side view of the integrated sample processing consumable of FIG. 1 for automated nucleic acid detection;
FIG. 3 is a rear view of the integrated sample processing consumable of FIG. 1 for automated nucleic acid detection;
FIG. 4 is a cross-sectional view taken along line A-A of the integrated sample processing consumable for automated nucleic acid testing of FIG. 1;
FIG. 5 is an enlarged schematic view of the junction of the lysis chamber and the extraction chamber of the integrated sample processing consumable for automated nucleic acid detection of FIG. 4.
In the figure, 1, a tube body; 2. a main body tube; 3. an amplification reaction tube; 4. a lysis chamber; 5. an extraction chamber; 6. an amplification chamber; 7. a communication channel; 8. a valve core; 9. a valve bore; 10. a gas channel; 11. a sealing plug; 12. a filtration membrane; 13. a pipe cap; 14. and fixing the clamping table.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the utility model but are not intended to limit the scope of the utility model.
The preferred embodiment of the integrated sample processing consumable for automated nucleic acid detection of the present invention is shown in fig. 1 to 5, and the integrated sample processing consumable for automated nucleic acid detection comprises a tube body 1, wherein a lysis chamber 4, an extraction chamber 5 and an amplification chamber 6 are sequentially arranged in the tube body 1 from top to bottom, and the lysis chamber 4 is used for accommodating a lysis solution to cause a lysis reaction of a nucleic acid sample; the extraction cavity 5 is used for accommodating an extracting solution, and the extracting solution is a diluent and plays a role in dilution; the amplification chamber 6 is used to contain solid-state enzymes that produce an amplification reaction, and serves to facilitate amplification.
The tube body 1 is a hollow structure, and the cracking cavity 4, the extraction cavity 5 and the amplification cavity 6 are all positioned in the hollow cavity of the tube body 1. The tube body 1 comprises a main tube 2 and an amplification reaction tube 3 arranged at the bottom of the main tube 2, the amplification reaction tube 3 is detachably connected with the main tube 2, and an amplification cavity 6 is formed in the inner cavity of the amplification reaction tube 3. In this embodiment, the amplification reaction tube 3 and the main tube 2 are connected by a ring-shaped clamping platform; the detachable connection facilitates the nucleic acid detection all-in-one machine to dismantle the amplification reaction tube 3 and obtain amplification information from the amplification reaction tube, so as to obtain a detection result.
The top of the main tube 2 is detachably connected with a cap 13 through a thread structure, that is, the cap 13 covers the top of the cracking chamber 4 of the tube body 1. After gathering the detection sample, operating personnel can demolish pipe cap 13 through helicitic texture, arrange the sample in schizolysis chamber 4, and the pipe cap 13 of screwing again keeps schizolysis chamber 4 to be a encapsulated situation, then puts into nucleic acid detection all-in-one with this integral type sample processing consumptive material. The fixing clamping table 14 extending along the circumferential direction of the pipe body 1 is further arranged outside the cracking cavity 4, the integrated sample treatment consumable is placed into the nucleic acid detection integrated machine, the nucleic acid detection integrated machine is fixed with the fixing clamping table in a clamping mode, and the integrated sample treatment consumable is positioned and prevented from moving.
The tube body 1 is formed with communicating channels 7 between the cracking cavity 4 and the extraction cavity 5 and between the extraction cavity 5 and the amplification reaction tube 3, and the communicating channels 7 enable the cracking cavity 4, the extraction cavity 5 and the amplification reaction tube 3 to be communicated with each other. A valve core 8 is arranged in the communication channel 7, and the valve core 8 is used for controlling the opening and closing of the communication channel 7 so that the detection sample enters the extraction cavity 5 and the amplification reaction tube 3 from the cracking cavity 4 in sequence.
The pipe body 1 is provided with a mounting groove, the mounting groove is perpendicular to the communication channel 7 and is assembled in the mounting groove in a sliding mode along the axial direction of the mounting groove. The valve core 8 is provided with a valve hole 9, and the extending direction of the valve hole 9 is perpendicular to the moving direction of the valve core 8, namely, the valve hole 9 and the communication channel 7 are parallel to each other. The valve holes 9 are two in number, the two valve holes 9 are parallel to each other and are arranged at intervals along the moving direction of the valve core 8, so that the valve holes 9 can be communicated with the communication channel 7 when the valve core 8 moves towards different directions.
The tube body 1 is further provided with a gas passage 10 communicated with the extraction cavity 5, the gas passage 10 is provided with a sealing plug 11, and the gas passage 10 is used for communicating the nucleic acid detection all-in-one machine with the extraction cavity 5 so as to ventilate or exhaust the extraction cavity 5, change the pressure in the extraction cavity 5 and drive the nucleic acid sample to move in different cavities by utilizing the change of the pressure.
The cracking cavity 4 is assembled with the extraction cavity 5 and the valve core 8 and the installation groove between the extraction cavity 5 and the amplification cavity 6 in a sliding manner, when the cracking reaction of the nucleic acid sample in the cracking cavity 4 is completed, the nucleic acid detection all-in-one machine pushes the cracking cavity 4 and the valve core 8 between the extraction cavity 5, the communication channel 7 between the cracking cavity 4 and the extraction cavity 5 is opened, and the valve core 8 between the extraction cavity 5 and the amplification cavity 6 is closed at the same time. The needle of the nucleic acid detection all-in-one machine is inserted into a sealing plug 11 of a sealing gas channel 10, the extraction cavity 5 is exhausted and vacuumized, at the moment, a communication channel 7 between the cracking cavity 4 and the extraction cavity 5 is opened, and a nucleic acid sample enters the extraction cavity 5 under the action of negative pressure. After the nucleic acid sample is diluted in the extraction cavity 5, the nucleic acid detection all-in-one machine pushes the valve core 8 between the extraction cavity 5 and the amplification cavity 6 and the valve core 8 between the reverse cracking cavity 4 and the extraction cavity 5, the communication channel 7 between the extraction cavity 5 and the amplification cavity 6 is opened, and the communication channel 7 between the cracking cavity 4 and the extraction cavity 5 is closed. The needle of the nucleic acid detection all-in-one machine is inserted into a sealing plug 11 of a sealing gas channel 10, the extraction cavity 5 is ventilated, the pressure in the extraction cavity 5 is increased, the top of the amplification cavity 6 is provided with a vent hole communicated with the atmosphere, the pressure in the amplification cavity 6 is always the same as the atmospheric pressure, and a nucleic acid sample enters the amplification cavity 6 under the action of the atmospheric pressure.
A filter membrane 12 is also preset in the cracking chamber 4, the filter membrane 12 is arranged at the inlet of the communication channel 7 at the bottom of the cracking chamber 4, a plurality of micropores are distributed in the filter membrane 12 and used for filtering the liquid in the cracking chamber 4, and only the filtered liquid can enter the extraction chamber 5.
The cracking cavity 4, the valve core 8 and the gas channel 10 are coaxially arranged along the up-down direction of the tube body 1, and the valve core 8 and the gas channel 10 are parallel to each other; draw chamber 5 and schizolysis chamber 4 upper and lower upwards eccentric arrangement, draw the central line arrangement of chamber 5 skew schizolysis chamber 4 promptly, gas passage 10 arranges at the top of schizolysis chamber 4 to the skew direction of schizolysis chamber 4 and the extending direction mutually perpendicular of gas passage 10, the purpose cooperates the structure of current nucleic acid detection all-in-one, also satisfies the mould production technology needs simultaneously. In other embodiments, the lysis chamber 4, the extraction chamber 5, and the amplification chamber 6 may be coaxially arranged to accommodate different integrated nucleic acid detection machines.
The working process of the utility model is as follows: the operating personnel is from the internal collection nucleic acid sample of patient, unscrews the pipe cap 13 on the body 1, arranges the nucleic acid sample in the schizolysis chamber 4, covers pipe cap 13, places the integral type sample processing consumptive material in the integrative machine of nucleic acid detection again, then starts the nucleic acid detection all-in-one.
The nucleic acid sample is subjected to a cracking reaction in the cracking cavity 4, meanwhile, the nucleic acid sample in the cracking cavity 4 is heated by a heating module in the nucleic acid detection integrated machine, and after the temperature rises to a set temperature, the cracking reaction is accelerated.
After the cracking reaction is completed, the nucleic acid detection all-in-one machine pushes the cracking cavity 4 and the valve core 8 between the extraction cavities 5, the communication channel 7 between the cracking cavity 4 and the extraction cavities 5 is opened, the needle of the nucleic acid detection all-in-one machine is inserted into the sealing plug 11 of the gas channel 10, the extraction cavities 5 are vacuumized by the needle, and liquid after cracking enters the extraction cavities 5 through the filtration of the filtration membrane 12 under the action of negative pressure.
The nucleic acid sample is diluted by the diluent in the extraction cavity 5, and after the extraction and dilution are finished, the nucleic acid detection all-in-one machine pushes a valve core 8 between the extraction cavity 5 and the amplification cavity 6, and a communication channel 7 between the extraction cavity 5 and the amplification cavity 6 is opened. The needle head of the nucleic acid all-in-one machine ventilates the extraction cavity 5, increases the pressure in the extraction cavity 5, and the liquid in the extraction cavity 5 enters the amplification cavity 6 under the action of the pressure.
The liquid is subjected to amplification reaction in the amplification cavity 6 according to the setting of the nucleic acid detection all-in-one machine, and detection is completed.
To sum up, the embodiment of the utility model provides an integrated sample processing consumable for automated nucleic acid detection, wherein a cracking cavity, an extraction cavity and an amplification cavity are all arranged in a tube body, when the nucleic acid detection is carried out, the cracking, extraction dilution and amplification reaction of a sample are all carried out under the condition of sealing in corresponding cavities, when the nucleic acid detection is carried out, corresponding valve cores are opened, the nucleic acid detection all-in-one machine utilizes a gas channel to ventilate and exhaust the extraction cavity, and because the extraction cavity is communicated with the communication channel, the change of the pressure in the extraction cavity drives the nucleic acid sample to flow in different cavities, so that the multi-step operations of PCR cracking, extraction, amplification, detection and the like can be completed, the sample does not need to be transferred among different instruments, and the sample, personnel and the environment are prevented from being polluted.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (9)
1. The utility model provides an integral type sample processing consumptive material for automizing nucleic acid testing, its characterized in that, includes the body, from top to bottom has arranged the schizolysis chamber in proper order in the body, has drawed the chamber and has enlarged the chamber, the schizolysis chamber is used for holding the lysate, it is used for holding the diluent to draw the chamber, the body in the schizolysis chamber with draw between the chamber, draw the chamber with it has the intercommunication passageway all to take the shaping between the chamber to enlarge, the last movable arrangement of intercommunication passageway has been used for controlling the case of intercommunication passageway switching, still arranged on the body with draw the gas channel of chamber intercommunication, the gas channel be used for supplying nucleic acid testing all-in-one to draw the intracavity to ventilate or exhaust in order to drive the nucleic acid sample to flow.
2. The integrated sample processing consumable for automated nucleic acid detection according to claim 1, wherein a mounting groove perpendicular to the communication channel is arranged on the tube body, the valve element is slidably fitted in the mounting groove along an axial direction of the mounting groove, and a valve hole is arranged on the valve element, and an extending direction of the valve hole is perpendicular to a moving direction of the valve element.
3. The integrated sample processing consumable for automated nucleic acid detection according to claim 2, wherein the valve holes are two in total, and the two valve holes are arranged in parallel and spaced apart from each other.
4. Integrated sample treatment consumable for automated nucleic acid detection according to any one of claims 1 to 3, characterised in that a filter membrane is also foreseen inside the lysis chamber.
5. The integrated sample processing consumable for automated nucleic acid detection according to claim 4, wherein the filter membrane is arranged at the inlet of the communicating channel at the bottom of the lysis chamber.
6. The integrated sample processing consumable of any one of claims 1-3, wherein the tube body comprises a main tube and an amplification reaction tube detachably connected to the bottom of the main tube, the inner cavity of the amplification reaction tube forms the amplification chamber, and the lysis chamber and the extraction chamber are located in the main tube.
7. The integrated sample processing consumable for automated nucleic acid testing according to any one of claims 1 to 3, wherein a fixing chuck extending along the circumference of the tube body is provided outside the lysis chamber.
8. The integrated sample processing consumable of any one of claims 1-3, wherein the lysis chamber and the valve cartridge are both arranged coaxially with the gas channel and the extraction chamber is arranged off-center from the centerline of the lysis chamber.
9. The integrated sample processing consumable for automated nucleic acid testing according to any one of claims 1 to 3, wherein a cap is removably arranged on the top of the tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121201483.3U CN216947013U (en) | 2021-05-31 | 2021-05-31 | Integrated sample treatment consumable for automatic nucleic acid detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121201483.3U CN216947013U (en) | 2021-05-31 | 2021-05-31 | Integrated sample treatment consumable for automatic nucleic acid detection |
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| Publication Number | Publication Date |
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| CN216947013U true CN216947013U (en) | 2022-07-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121201483.3U Active CN216947013U (en) | 2021-05-31 | 2021-05-31 | Integrated sample treatment consumable for automatic nucleic acid detection |
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| CN (1) | CN216947013U (en) |
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2021
- 2021-05-31 CN CN202121201483.3U patent/CN216947013U/en active Active
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