CN216891018U

CN216891018U - Circular shaking table for shaking cell culture bottle at high speed

Info

Publication number: CN216891018U
Application number: CN202220182013.5U
Authority: CN
Inventors: 史文佳; 黄菊芳; 陈旦; 夏明波
Original assignee: Central South University
Current assignee: Central South University
Priority date: 2022-01-21
Filing date: 2022-01-21
Publication date: 2022-07-05
Anticipated expiration: 2032-01-21

Abstract

The utility model provides a circular shaking table for shaking a cell culture bottle at a high speed, which comprises a tray, wherein a structure for fixing the culture bottle is distributed on the tray, the tray is rotatably connected with a box body and is also in transmission connection with a rotary driving part in the box body, the rotary driving part is powered by a power supply in the box body, and the box body can be placed in a cell culture box. The culture bottle is supported and fixed by the tray, the culture bottle can be rotated at a high speed and shaken under the drive of the rotary drive part to enable cells attached to the upper layer to fall off, and the purpose of purifying primary cells is finally achieved.

Description

Circular shaking table for shaking cell culture bottle at high speed

Technical Field

The utility model relates to the field of biological culture instruments, in particular to a circular shaking table for shaking a cell culture bottle at a high speed.

Background

Currently, in the field of primary cell extraction, cell purification is often required due to the variety of cell types in tissues. Purification of cells by mechanical shaking is a common purification method. For example, in the process of extracting primary retinal astrocytes, 7-10 days after rat retinal digest cell suspension of newborn 1-2 days is injected into a T25 culture flask, lower layer astrocytes are gradually attached to the wall and spread on the bottom of the culture flask, and upper layer microglia are attached to the conglomerate layer. Literature indicates that shaking the flask at high speed (about 180rpm/min) for 1.5 hours removes most of the adherent microglia; while shaking overnight removed most microglia and oligodendrocytes.

In the experimental process, it is found that if the culture bottle is placed on an instrument outside the cell culture box and shaken, not only is the risk of cell contamination present, but also the temperature, humidity and O of the air during the purification process₂/CO₂The ratio of (A) is not suitable for cell survival, and also has an adverse effect on the growth state of astrocytes. After inspection, no small high-speed cell shaking instrument which can be put into a cell incubator to work exists in the prior art.

It should be noted that the above background description is only for the convenience of clear and complete description of the technical solutions of the present application and for the understanding of those skilled in the art. Such solutions are not considered to be known to the person skilled in the art merely because they have been set forth in the background section of the present application.

SUMMERY OF THE UTILITY MODEL

The purpose of the utility model is: aiming at the defects in the background technology, the small high-speed cell shaking instrument for cell purification is provided, can be placed in a cell culture box, and can shake a culture flask at a high speed for a long time to purify cells, so that the damage to the cells in the purification process is avoided, the extraction efficiency and purity of primary cells are improved, and the cell state is ensured.

In order to achieve the purpose, the utility model provides a circular shaking table for shaking a cell culture bottle at a high speed, which comprises a tray, wherein a structure for fixing the cell culture bottle is distributed on the tray, the tray is rotationally connected with a box body and is also in transmission connection with a rotary driving part in the box body, the rotary driving part is powered by a power supply in the box body, and the box body can be placed in a cell culture box.

Further, the tray is connected with the buffer disc through a first supporting rod, the buffer disc is connected with the rotating disc through a second supporting rod, and the rotating disc is rotatably connected with the box body.

Further, the lateral wall of box is equipped with LED display screen, power button and control knob, the rotation driving portion is DC brushless motor, power button can start DC brushless motor, control knob includes rotational speed knob and time knob, can control respectively DC brushless motor's rotational speed and operating duration.

Furthermore, a battery jar is arranged at the bottom of the box body, and a dry battery for supplying power to the direct current brushless motor is installed in the battery jar.

Further, the structure for fixing the culture bottle is a fixing rope.

Further, the fixing rope is arranged in a net shape and has elasticity.

Further, the lower surface of box is connected with the base, the bottom of base is equipped with anti-skidding rubber pad.

The scheme of the utility model has the following beneficial effects:

according to the circumferential shaking table for shaking the cell culture bottle at the high speed, the culture bottle is supported and fixed by virtue of the tray, the culture bottle can rotate at the high speed and shake under the driving of the rotary driving part, so that cells attached to the upper layer can fall off, and the purpose of purifying primary cells is finally achieved;

other advantages of the present invention will be described in detail in the detailed description that follows.

Drawings

FIG. 1 is a three-dimensional schematic diagram of the overall structure of the present invention;

FIG. 2 is an elevational view of the overall construction of the present invention;

fig. 3 is a top view of the tray of the present invention.

[ description of reference ]

1-a tray; 2-fixing the rope; a first support bar; 4-a buffer tray; 5-a second support bar; 6-rotating the disc; 7-a box body; 8-a DC brushless motor; 9-LED display screen; 10-power button; 11-a control knob; 12-a battery well; 13-a base; 14-antiskid rubber pad.

Detailed Description

In order to make the technical problems, technical solutions and advantages of the present invention more apparent, the following detailed description is given with reference to the accompanying drawings and specific embodiments. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

As shown in FIGS. 1 to 3, the embodiment of the present invention provides a circular rocking bed for rocking a cell culture flask at a high speed, comprising a tray 1, wherein a fixing string 2 is connected to the tray 1, and the flask is fixed to the tray 1 by the fixing string 2, thereby ensuring that the flask is not displaced when rotating at a high speed. The lower surface of tray 1 is connected with buffer tray 4 through first bracing piece 3, and the lower surface of buffer tray 4 then is connected with rotary disk 6 through second bracing piece 5, and rotary disk 6 rotates with box 7 to be connected, and 6 bottom of rotary disk are connected with the transmission of direct current brushless motor 8, and direct current brushless motor 8 is located box 7 equally.

Therefore, under the driving of the brushless DC motor 8, the rotating disc 6, the buffer disc 4 and the tray 1 can rotate and shake the culture flask at a high speed to drop the cells attached to the upper layer, and finally achieve the purpose of primary cell purification. Further, the shaking can be reduced by the arrangement of the buffer tray 4, the rotary tray 6, and the like, and the culture bottle can be more stably rotated and shaken.

Wherein, the lateral wall of box 7 is equipped with LED display screen 9, power button 10 and control knob 11, and power button 10 can start DC brushless motor 8, and control knob 11 includes rotational speed knob and time knob, can control DC brushless motor's rotational speed and operating duration respectively. The bottom of the box body 7 is provided with a battery jar 12, and a dry battery for supplying power to the direct current brushless motor 8 is arranged in the battery jar 12. The PWM direct current speed regulating system can control the rotating speed of the direct current brushless motor 8, and the rotating speed is displayed on the LED display screen 9 through the photoelectric sensor.

Consequently, the instrument that this embodiment provided can use the dry battery power supply, avoids need to pull out the plug wire and connects the power after putting into the cell culture case, and this instrument volume is less simultaneously, can disinfect the back and put into the cell culture case and use, can guarantee the stability of sterile environment in cell culture case and the blake bottle during the purification to guarantee the growing environment of cell, guarantee that the cell is in good growth state.

As a further improvement, the fixing rope 2 is provided with a net shape and has certain elasticity, the culture bottles can be put on the tray 1 from the net holes, and the culture bottles are pressed on the tray 1 by means of the elasticity of the fixing rope 2 in a flat-lying posture.

As a further improvement, in the present embodiment, a plurality of bases 13 are connected to the lower surface of the box 7, and the bottom ends of the bases 13 are provided with anti-slip rubber pads 14, so that the whole apparatus can be placed in the cell culture box more stably and reliably without displacement due to rotation of the tray 1.

In specific use, need keep flat the blake bottle on tray 1 and rely on fixed rope 2 fixed, press power button 10 start device, rotate control knob 11's rotational speed knob, adjust DC brushless motor 8 to suitable rotational speed through observing LED display screen 9, let it drive rotary disk 6 etc. rotatory, finally drive tray 1 rotatory, rotatory with high-speed, rock the blake bottle, make the adnexed cell in upper strata drop, finally reach the purpose of primary cell purification.

While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the utility model as defined in the appended claims.

Claims

1. The utility model provides a circumference type shaking table of cell culture bottle shakes at a high speed, a serial communication port, includes the tray, laid the structure fixed to the blake bottle on the tray, the tray rotates with the box to be connected, is connected with the transmission of the rotation driving portion in the box simultaneously, rotation driving portion passes through power supply in the box, the box can be placed in the cell culture case.

2. The rocking platform of claim 1, wherein the tray is connected to a buffer tray via a first support rod, the buffer tray is connected to a rotating plate via a second support rod, and the rotating plate is rotatably connected to the housing.

3. The rocking device according to claim 1, wherein the sidewall of the housing has an LED display, a power button and a control knob, the rotary driving unit is a brushless DC motor, the power button can start the brushless DC motor, and the control knob comprises a speed knob and a time knob, which can control the speed and the operation time of the brushless DC motor respectively.

4. The rocking platform of claim 3, wherein the bottom of the housing has a battery compartment, and a dry battery is installed in the battery compartment for supplying power to the brushless DC motor.

5. The rocking device according to claim 1, wherein the means for securing the flask is a securing string.

6. The rocking device according to claim 5, wherein the fixing string is formed in a net shape and has elasticity.

7. The rocking peripheral bed for shaking a cell culture flask at a high speed according to claim 1, wherein a base is attached to the lower surface of the housing, and a rubber pad is provided at the bottom of the base to prevent slipping.