CN216864142U - Energy-saving controllable high-density culture device for Beauveria bassiana - Google Patents

Abstract

The utility model discloses an energy-saving controllable high-density culture device for Beauveria bassiana, belonging to the technical field of strain culture, comprising a shell, wherein the shell is provided with a cover body and a liquid inlet, the top in the shell is provided with a culture medium container and a liquid inlet pipe, the cover body is clamped on the culture medium container, and the liquid inlet is communicated with the liquid inlet pipe. The strain culture is simpler and more convenient, and the manpower and the time are saved.

Description

Energy-saving controllable high-density culture device for Beauveria bassiana

Technical Field

The utility model relates to the technical field of strain culture, in particular to an energy-saving controllable high-density culture device for Beauveria bassiana.

Background

The oospore muscardine fungi is a microorganism belonging to Beauveria, has a Chinese origin, is mainly used for scientific research and teaching, is cultured manually in most of the prior art, needs a great deal of time for manual culture operation in the culture mode, and has complicated culture operation steps.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an energy-saving controllable high-density culture device for beauveria bassiana, which is used for solving the problems that a large amount of time is consumed manually for culture operation and the culture operation steps are complicated and complicated in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides an energy-conserving controllable high density culture apparatus of beauveria bassiana, which comprises a housin, be equipped with lid and inlet on the casing, the top is equipped with culture medium container and feed liquor pipe in the casing, and the lid card establishes on culture medium container, inlet and feed liquor pipe intercommunication, and install vibrating motor on the casing upper side lateral wall, be equipped with the frame plate on vibrating motor's the output, and be equipped with mixed household utensils in the frame plate, mixed household utensils are located the below of feed liquor pipe, and the casing inside and outside is equipped with dona tub, fermentation cylinder respectively with the bottom, the play liquid end of mixed household utensils passes through the pipeline and is connected with the feed liquor end of dona tub, and the play liquid end of dona tub passes through the pipeline and is connected with the feed liquor end of fermentation cylinder, the air inlet of dona tub and fermentation cylinder is connected with intake pipe one, intake pipe two respectively, and connects the culture solution input tube on the dona tub.

Preferably, the middle part in the casing still is equipped with the water pump, and the feed liquor end and the water pipe intercommunication of water pump, the play liquid end of water pump is connected with the end of intaking of seedtank, fermentation cylinder respectively through the pipeline, and is equipped with valve one, valve two respectively on the intake of seedtank and fermentation cylinder.

Preferably, a third valve is arranged at the liquid outlet end of the mixing vessel, a fourth valve is arranged at the air inlet of the seed tank, and a fifth valve is arranged at the liquid outlet end of the seed tank.

Preferably, all be equipped with in dona tub and the fermentation vat and wash the mechanism, and wash the mechanism and intake end intercommunication, wash the mechanism and include high pressure nozzle and shower nozzle mount pad, high pressure nozzle establishes on the shower nozzle mount pad, and has seted up the basin in the shower nozzle mount pad, basin and high pressure nozzle, the end intercommunication of intaking.

Preferably, the fermentation tank is provided with a motor, and an output shaft of the motor penetrates through the top of the fermentation tank and is connected with a stirring mechanism in the fermentation tank.

Compared with the prior art, the utility model has the beneficial effects that:

the utility model is suitable for strains to breed in the culture medium container, the bred strains and the culture solution are mixed and fed from the liquid inlet by manpower, the strains and the culture solution are mixed and grown in the mixing vessel, the strains are favorable for the growth of the strains, the mixed strains and the culture solution enter the seed tank for culture to generate a large amount of hyphae, nitrogen is introduced into the seed tank at the same time, the growth of the hyphae is favorable, and then the mixed strains enter the fermentation tank for secondary fermentation from the seed tank to obtain the strains with thick and strong hyphae, the whole process only participates in one working procedure by manpower, so that the strain culture is simpler and more convenient, and the manpower and the time are saved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the internal structure of the housing according to the present invention;

FIG. 3 is a schematic view of the internal structure of a fermenter according to the present invention.

In the figure: 1. a housing; 2. a cover body; 3. a liquid inlet; 4. a water pipe; 5. a second air inlet pipe; 6. a second valve; 7. a culture medium container; 8. a vibration motor; 9. a frame plate; 10. a mixing vessel; 11. a seed tank; 12. a first air inlet pipe; 13. a culture solution input pipe; 14. a fermentation tank; 15. a motor; 16. a water pump; 17. a high pressure spray head; 18. a first valve; 19. and a stirring mechanism.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Example (b):

referring to fig. 1-3, the present invention provides a technical solution: an energy-saving controllable high-density culture device for beauveria bassiana comprises a shell 1, wherein a cover body 2 and a liquid inlet 3 are arranged on the shell 1, a culture medium container 7 and a liquid inlet pipe are arranged at the top in the shell 1, a liquid or solid culture medium is placed in the culture medium container 7, a strain is inoculated into the culture medium container 7 for preliminary culture, the cover body 2 is clamped on the culture medium container 7, the liquid inlet 3 is communicated with the liquid inlet pipe, the strain and the culture solution are added from the liquid inlet 3 and flow out through the liquid inlet pipe, a vibration motor 8 is arranged on the side wall of the upper portion in the shell 1, a frame plate 9 is arranged on the output end of the vibration motor 8, a mixing vessel 10 is arranged in the frame plate 9, the vibration motor 8 works to generate vibration to drive the mixing vessel 10 to vibrate, the mixing vessel 10 vibrates to enable the strain to be scattered and uniformly mixed with the culture solution, the fermentation speed is accelerated, the mixing vessel 10 is positioned below the liquid inlet pipe, the bacterial and the culture solution flow into the mixing vessel 10 from the liquid inlet pipe, and below and bottom are equipped with dona tub 11, fermentation cylinder 14 respectively in the casing 1, the play liquid end of mixing vessel 10 passes through the pipeline and is connected with the feed liquor end of dona tub 11, dona tub 11 can cultivate the bacterial solution, and the play liquid end of dona tub 11 passes through the pipeline and is connected with the feed liquor end of fermentation cylinder 14, bacterial solution after the process of cultivateing flows into fermentation cylinder 14 in, carries out the second grade fermentation, obtains the stout bacterial of hypha, fermentation cylinder 14 rear is equipped with out the liquid end, and goes out the liquid end and run through casing 1 and outwards extend for the bacterial and the culture solution that the fermentation of discharging accomplished, the air inlet of dona tub 11 and fermentation cylinder 14 respectively with intake pipe one 12, intake pipe two 5 are connected, and connect culture solution input tube 13 on the dona tub 11.

The middle part in the shell 1 is also provided with a water pump 16, the liquid inlet end of the water pump 16 is communicated with a water pipe 4, the liquid outlet end of the water pump 16 is respectively connected with the water inlet ends of the seed tank 11 and the fermentation tank 14 through pipelines, the water pump 16 can send the disinfectant in the water pipe 4 into the seed tank 11 and the fermentation tank 14 and spray out through a high-pressure spray nozzle 17 to wash and disinfect the inner walls of the seed tank 11 and the fermentation tank 14, the water inlet ends of the seed tank 11 and the fermentation tank 14 are respectively provided with a valve I18 and a valve II 6, the liquid outlet end of the mixing vessel 10 is provided with a valve III, the air inlet of the seed tank 11 is provided with a valve IV, the liquid outlet end of the seed tank 11 is provided with a valve V, the seed tank 11 and the fermentation tank 14 are both internally provided with a washing mechanism, the washing mechanism is communicated with the water inlet end, the washing mechanism comprises a high-pressure spray nozzle 17 and a spray nozzle mounting seat, the high-pressure spray nozzle 17 is arranged on the spray nozzle mounting seat, and the basin has been seted up in the shower nozzle mount pad, basin and high pressure nozzle 17, the end intercommunication of intaking, be equipped with motor 15 on the fermentation cylinder 14, and the output shaft of motor 15 runs through the fermentation cylinder 14 top and is connected with the rabbling mechanism 19 in the fermentation cylinder 14, and motor 15 work can drive the rotation of rabbling mechanism 19 for the bacterial dispersion fermentation in the fermentation cylinder 14 fully absorbs the culture solution.

The working principle is as follows: liquid or solid culture medium is placed in a culture medium container 7, strains are inoculated into the culture medium container 7 for primary culture, a cover body 2 is covered on the culture medium container 7 to form a closed space in the culture medium container 7, the cultured strains and culture solution are manually placed into a liquid inlet 3 and flow into a mixing vessel 10 from a liquid inlet pipe, a vibration motor 8 works to generate vibration to drive the mixing vessel 10 to vibrate, the mixing vessel 10 vibrates to enable the strains to be dispersed and uniformly mixed with the culture solution, a valve III at a liquid outlet end of the mixing vessel 10 is opened after the culture is finished, the strains and the culture solution flow into a seed tank 11, nitrogen is introduced into the seed tank 11 from an air inlet pipe I12 to facilitate the growth of the strains, the culture solution with higher concentration can be added into the seed tank 11 from a culture solution input pipe 13, a valve V at a liquid outlet end of the seed tank 11 is opened after the culture is finished, the strains and the culture solution flow into a fermentation tank 14, simultaneously, nitrogen is introduced into the fermentation tank 14 through the air inlet pipe II 5, secondary fermentation is carried out in the fermentation tank 14, a strain with thick hyphae is obtained, the motor 15 can drive the stirring mechanism 19 to rotate when working, so that the strain in the fermentation tank 14 is subjected to dispersive fermentation, culture solution is fully absorbed, the water pump 16 can send disinfectant water in the water pipe 4 into the seed tank 11 and the fermentation tank 14, and the disinfectant water is sprayed out through the high-pressure spray nozzle 17 to wash and disinfect the inner walls of the seed tank 11 and the fermentation tank 14.

While there have been shown and described the fundamental principles and essential features of the utility model and advantages thereof, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof; the present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. The utility model provides an energy-conserving controllable high density culture apparatus of beauveria bassiana, includes casing (1), its characterized in that: be equipped with lid (2) and inlet (3) on casing (1), casing (1) interior top is equipped with culture medium container (7) and feed liquor pipe, and lid (2) card is established on culture medium container (7), inlet (3) and feed liquor pipe intercommunication, and install vibrating motor (8) on the lateral wall of top in casing (1), be equipped with frame plate (9) on vibrating motor (8)'s the output, and be equipped with in frame plate (9) and mix household utensils (10), mix household utensils (10) and be located the below of feed liquor pipe, and casing (1) interior below and bottom are equipped with dona tub (11), fermentation cylinder (14) respectively, the play liquid end of mixing household utensils (10) passes through the pipeline and is connected with the feed liquor end of dona tub (11), and the play liquid end of dona tub (11) passes through the pipeline and is connected with the feed liquor end of fermentation cylinder (14), the air inlet of dona tub (11) and fermentation cylinder (14) respectively with intake pipe (12), The second air inlet pipe (5) is connected, and the seeding tank (11) is connected with a culture solution input pipe (13).

2. The energy-saving controllable high-density culture device for the beauveria bassiana according to claim 1, characterized in that: the middle part still is equipped with water pump (16) in casing (1), and the inlet end and water pipe (4) intercommunication of water pump (16), the play liquid end of water pump (16) is connected with the end of intaking of seedtank (11), fermentation cylinder (14) respectively through the pipeline, and is equipped with valve one (18), valve two (6) on the end of intaking of seedtank (11) and fermentation cylinder (14) respectively.

3. The energy-saving controllable high-density culture device for the beauveria bassiana according to claim 1, characterized in that: a third valve is arranged at the liquid outlet end of the mixing vessel (10), a fourth valve is arranged at the air inlet of the seed tank (11), and a fifth valve is arranged at the liquid outlet end of the seed tank (11).

4. The energy-saving controllable high-density culture device for the beauveria bassiana according to claim 2, characterized in that: all be equipped with in dona tub (11) and fermentation cylinder (14) and wash the mechanism, and wash the mechanism and intake water and hold the intercommunication, wash the mechanism and include high pressure nozzle (17) and shower nozzle mount pad, high pressure nozzle (17) are established on the shower nozzle mount pad, and have seted up the basin in the shower nozzle mount pad, basin and high pressure nozzle (17), the end intercommunication of intaking.

5. The energy-saving controllable high-density culture device for the beauveria bassiana according to claim 1, characterized in that: the fermentation tank (14) is provided with a motor (15), and an output shaft of the motor (15) penetrates through the top of the fermentation tank (14) and is connected with a stirring mechanism (19) in the fermentation tank (14).

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