CN217809395U

CN217809395U - Simple container convenient for rapid propagation of microbial strains

Info

Publication number: CN217809395U
Application number: CN202221098951.3U
Authority: CN
Inventors: 刘仁绿; 贺根和; 宋勇生; 廖永辉; 尹丽; 彭世星
Original assignee: Jinggangshan University
Current assignee: Jinggangshan University
Priority date: 2022-05-09
Filing date: 2022-05-09
Publication date: 2022-11-15
Anticipated expiration: 2032-05-09

Abstract

The utility model provides a simple and easy container convenient to microbial srain expands banks up fast, includes cultivates the jar, it has the top cap to articulate on the cultivation jar, cultivation jar one side is provided with the air pump, the end fixedly connected with coupling hose that gives vent to anger of air pump, coupling hose keeps away from the one end fixedly connected with ring pipe of cultivation jar, and the coupling hose middle part is provided with aseptic filter, ring array distributes and has a plurality of outlet ducts on the ring pipe, the one end that the ring pipe was kept away from to the outlet duct runs through inside the cultivation jar lateral wall extends to the cultivation jar, the utility model discloses an in letting in aseptic air to the cultivation jar, on the one hand stirs the nutrient solution, is favorable to microbial srain and nutrient solution misce bene, and oxygen in the aseptic air of on the other hand dissolves at the nutrient solution for the bacterial obtains sufficient oxygen, promotes microbial srain growth, has improved the efficiency of expanding banks up with earth of microbial srain.

Description

Simple container convenient for rapid propagation of microbial strains

Technical Field

The utility model belongs to the technical field of the microorganism bacterial species expands banks up with earth, specifically is a simple and easy container convenient to microorganism bacterial species expands banks up with earth fast.

Background

In the microbial fermentation production, in order to ensure a sufficient inoculation amount, the microorganisms are usually cultured by using an expanding culture device. Present microorganism expands banks with earth device is not convenient for carry out even dispersion to the bacterial in the culture tank and nutrient solution, expands to bank with earth the effect relatively poor, also is not convenient for increase dissolved oxygen volume simultaneously, leads to microorganism growth rate slower, obviously can't satisfy people's user demand, consequently, we propose a simple and easy container that microorganism fungus kind of being convenient for expands fast and banks with earth.

SUMMERY OF THE UTILITY MODEL

The utility model aims to improve and innovate the defects and problems existing in the background technology and provide a simple container convenient for the rapid propagation of microbial strains.

The utility model provides a simple and easy container convenient to microbial srain expands banks up with earth fast, includes cultivates the jar, it has the top cap to articulate on the cultivation jar, cultivation jar one side is provided with the air pump, the end fixedly connected with coupling hose that gives vent to anger of air pump, coupling hose keeps away from the one end fixedly connected with ring pipe of cultivateing the jar, and the coupling hose middle part is provided with aseptic filter, ring array distributes on the ring pipe has a plurality of outlet ducts, the one end that the ring pipe was kept away from to the outlet duct runs through inside the cultivation jar lateral wall extends to the cultivation jar.

The further proposal is that the air outlet pipe is provided with a check valve.

The further proposal is that the air outlets of the air outlet pipes are inclined upwards or downwards, and the inclined directions of the air outlets of the adjacent air outlet pipes are different.

The further scheme is that a rotating motor is installed on the bottom wall of the culture tank, a stirring shaft is fixedly connected with the output end of the rotating motor, the stirring shaft extends to the upper end of the inner cavity of the culture tank through a through hole formed in the bottom of the culture tank, a bearing and a sealing ring which are in running fit with the outer surface of the stirring shaft are installed on the inner wall of the through hole, and a helical blade and a stirring blade are arranged on the stirring shaft located in the inner cavity of the culture tank.

Further, the side wall of the culture tank is provided with an observation window.

The further scheme is that a handle is arranged on the upper surface of the top cover.

The further proposal is that supporting legs are arranged at four corners of the lower surface of the culture tank.

The further proposal is that a liquid discharge pipe is arranged on the bottom wall of the culture tank, and a stop valve is arranged on the liquid discharge pipe.

Compared with the prior art, the beneficial effects of the utility model are that: (1) Under the action of an air pump, air sequentially passes through a connecting hose, a sterile filter, an annular pipe and an air outlet pipe and then is introduced into the culture tank, the sterile filter removes mixed bacteria in the air, the sterile air stirs the nutrient solution on one hand, so that microbial strains and the nutrient solution are mixed uniformly, and on the other hand, oxygen in the sterile air is dissolved in the nutrient solution, so that enough oxygen is obtained by strains, the growth and the propagation of the microbial strains are promoted, and the expanding culture efficiency of the microbial strains is improved;

(2) It is rotatory to drive helical blade and stirring paddle leaf through the rotating electrical machines, and stirring paddle leaf will cultivate jar edge and central point's nutrient solution misce bene, and helical blade will be located the nutrient solution of culture tank bottom and stir upwards, realizes that the nutrient solution is at vertical direction misce bene to obtain good cultivation environment, further improved the expand of microbial strains and banked up efficiency.

Drawings

FIG. 1 is a schematic cross-sectional view of the present invention;

fig. 2 is a schematic top view of the present invention;

FIG. 3 is a schematic view of the present invention at a position A in FIG. 1;

reference numerals: the device comprises a culture tank 1, a top cover 2, an air pump 3, a sterile filter 4, a connecting hose 5, an annular pipe 6, an air outlet pipe 7, a check valve 8, a rotating motor 9, a stirring shaft 10, a helical blade 11, a stirring paddle 12, an observation window 13, a liquid discharge pipe 14, a support leg 15, a through hole 16, a bearing 17, a sealing ring 18 and a handle 19.

Detailed Description

The present invention will be described in further detail with reference to fig. 1 to 3.

The utility model provides a simple and easy container convenient to microbial srain expands banks up with earth fast, includes culture tank 1, 1 lower surface four corners department of culture tank is provided with supporting leg 15, be provided with fluid-discharge tube 14 on the 1 diapire of culture tank, be provided with the trip valve on the fluid-discharge tube 14. It has top cap 2 to articulate on the culture tank 1, surface mounting has handle 19 on the top cap 2, be provided with observation window 13 on the culture tank 1 lateral wall, see through observation window 13 and be convenient for observe the expand circumstances of banking up with earth of microbial srain.

Referring to fig. 1 and 2, an air pump 3 is arranged on one side of the culture tank 1, a connection hose 5 is fixedly connected to an air outlet end of the air pump 3, an annular tube 6 is fixedly connected to one end, away from the culture tank 1, of the connection hose 5, an aseptic filter 4 is arranged in the middle of the connection hose 5, the aseptic filter 4 is used for removing sundry bacteria in the air, a plurality of air outlet pipes 7 are distributed on the annular tube 6 in an annular array manner, check valves 8 are arranged on the air outlet pipes 7, one ends, away from the annular tube 6, of the air outlet pipes 7 penetrate through the side wall of the culture tank 1 and extend into the culture tank 1, air outlets of the air outlet pipes 7 are inclined upwards or downwards, and the inclination directions of the air outlets of the adjacent air outlet pipes 7 are different.

Referring to fig. 1 and fig. 3, a rotating motor 9 is installed on the bottom wall of the culture tank 1, a stirring shaft 10 is fixedly connected to the output end of the rotating motor 9, the stirring shaft 10 extends to the upper end of the inner cavity of the culture tank 1 through a through hole 16 formed in the bottom of the culture tank 1, a bearing 17 and a sealing ring 18 which are in running fit with the outer surface of the stirring shaft 10 are installed on the inner wall of the through hole 16, the sealing ring 18 ensures the sealing performance of the culture tank 1, and a helical blade 11 and a stirring blade 12 are arranged on the stirring shaft 10 located in the inner cavity of the culture tank 1.

The utility model discloses a theory of operation: during the specific use, pour microbial srain and nutrient solution into culture tank 1, open air pump 3 and rotating electrical machines 9, the air is under the effect of air pump 3, pass through coupling hose 5 in proper order, aseptic filter 4, let in culture tank 1 behind annular pipe 6 and the outlet duct 7, aseptic filter 4 gets rid of the miscellaneous fungus in the air, aseptic air stirs the nutrient solution on the one hand, be favorable to microbial srain and nutrient solution misce bene, oxygen in the aseptic air on the other hand dissolves in the nutrient solution, make the bacterial obtain sufficient oxygen, microbial srain growth and reproduction has been promoted, rotating electrical machines 9 drives helical blade 11 and stirring paddle 12 is rotatory simultaneously, stirring paddle 12 will cultivate tank 1 edge and the nutrient solution misce of central point, helical blade 10 will be located culture tank 1 bottom's nutrient solution and stir upwards, realize that the nutrient solution is at horizontal direction and vertical direction misce bene, thereby good cultivation environment has been obtained, the expanding of microbial srain efficiency of cultivateing has been improved.

The above embodiments are used to illustrate the technical solutions of the present invention and not to limit the same, and those skilled in the art should understand that any modification, equivalent replacement or improvement made to the technical solutions of the present invention within the spirit and principle of the present invention are all included in the protection scope of the present invention.

Claims

1. The utility model provides a simple and easy container convenient to microbial srain expands cultivates fast, includes culture tank (1), its characterized in that: it has top cap (2) to articulate on cultivateing jar (1), it is provided with air pump (3) to cultivate jar (1) one side, the end fixedly connected with coupling hose (5) of giving vent to anger of air pump (3), coupling hose (5) are kept away from one end fixedly connected with ring conduit (6) of cultivateing jar (1), and coupling hose (5) middle part is provided with aseptic filter (4), ring array distributes on ring conduit (6) has a plurality of outlet ducts (7), the one end that ring conduit (6) were kept away from in outlet duct (7) runs through and cultivates jar (1) lateral wall and extend to cultivates jar (1) inside.

2. The simple container for facilitating rapid propagation of microbial strains according to claim 1, wherein: and the air outlet pipe (7) is provided with a check valve (8).

3. The simple container for facilitating rapid propagation of microbial strains according to claim 1, wherein: the air outlets of the air outlet pipes (7) are inclined upwards or downwards, and the inclined directions of the air outlets of the adjacent air outlet pipes (7) are different.

4. The simple container for facilitating rapid propagation of microbial strains according to any one of claims 1 to 3, wherein: install rotating electrical machines (9) on cultivating tank (1) diapire, the output end fixedly connected with (mixing) shaft (10) of rotating electrical machines (9), through-hole (16) that (mixing) shaft (10) were seted up through cultivating tank (1) bottom extend to cultivating tank (1) inner chamber upper end, install on through-hole (16) inner wall with (mixing) shaft (10) surface normal running fit's bearing (17) and sealing washer (18), be located and be provided with helical blade (11) and stirring paddle leaf (12) on (mixing) shaft (10) of cultivating tank (1) inner chamber.

5. The simple container for facilitating rapid propagation of microbial strains according to claim 1, wherein: an observation window (13) is arranged on the side wall of the culture tank (1).

6. The simple container for facilitating rapid propagation of microbial strains according to claim 1, wherein: and a handle (19) is arranged on the upper surface of the top cover (2).

7. The simple container for facilitating rapid propagation of microbial strains according to claim 1, wherein: supporting legs (15) are arranged at four corners of the lower surface of the culture tank (1).

8. The simple container for facilitating rapid propagation of microbial strains according to claim 1, wherein: a liquid discharge pipe (14) is arranged on the bottom wall of the culture tank (1), and a shut-off valve is arranged on the liquid discharge pipe (14).