CN216808803U

CN216808803U - Microorganism separation and purification device

Info

Publication number: CN216808803U
Application number: CN202122516411.4U
Authority: CN
Inventors: 廖兴刚; 孟泽彬; 罗倩
Original assignee: Guizhou Education University
Current assignee: Guizhou Education University
Priority date: 2021-10-19
Filing date: 2021-10-19
Publication date: 2022-06-24
Anticipated expiration: 2031-10-19

Abstract

The utility model relates to microorganism purification equipment, in particular to a microorganism separation and purification device, which comprises a box body, wherein the inner wall of the box body is fixedly connected with a supporting plate, the supporting plate is provided with a first through hole and a second through hole, the first through hole and the second through hole are respectively connected with a first base and a second base, the first base and the second base can be detachably connected with a culture dish scribing component, the lower surface of the supporting plate is provided with a first sliding groove and a second sliding groove which are vertical to each other and crossed, the inner sides of the first sliding groove and the second sliding groove are respectively provided with a first sliding block and a second sliding block which are connected with the supporting plate in a sliding manner, the first sliding block is fixedly connected with the first base, the second sliding block is fixedly connected with the second base, the outer surfaces of the first sliding block and the second sliding block are jointly and rotatably connected with a connecting block, the outer surface of the connecting block is rotatably connected with a transmission block, and one end of the transmission block is connected with a motor; by adopting the technical scheme of the utility model, the culture dish can be quickly shaken and drawn, the working efficiency is improved, and the culture dish is suitable for separating and purifying microorganisms.

Description

Microorganism separation and purification device

Technical Field

The utility model relates to microorganism purification equipment, in particular to a microorganism separation and purification device.

Background

The process of obtaining a microorganism containing only one or one strain of microorganism from a heterogeneous population of microorganisms is called isolation and purification of the microorganism. The separation and purification method includes dilution plate pouring, plate coating, plate drawing and dilution tube shaking, and the plate drawing method is usually adopted to separate and purify the microorganism. The microorganism plate scribing operation has very important significance in the fields of biology, medicine, sewage treatment and the like, and is the most basic and most common technical means in the operation processes of microbiological research, biological pharmacy, water quality detection and the like. The microorganism plate streaking separation method is that microbes mixed together or different cells in the same microbial population are streaked and diluted on the surface of a plate culture medium by using an inoculating loop to obtain a plurality of single cells which are distributed independently, and the single cells grow and propagate into a single colony after being cultured. The manual stirring and mixing is time-consuming and labor-consuming, the problem of uneven mixing is easily caused, and in order to shorten the period of microorganism separation and purification, the separation and purification can be completed by means of semi-automatic equipment or automatic equipment.

SUMMERY OF THE UTILITY MODEL

In order to solve the defects of the prior art, the utility model provides a microorganism separation and purification device, which aims to solve the problem of low efficiency when a flat plate line drawing method is adopted to separate and purify microorganisms in the prior art.

In order to achieve the purpose, the utility model provides the following basic scheme: a microorganism separation and purification device comprises a box body, wherein a supporting plate is fixedly connected to the inner wall of the box body, a first through hole and a second through hole which are perpendicular to each other and crossed are formed in the supporting plate, the first through hole and the second through hole are respectively connected with a first base and a second base, the first base and the second base can be detachably connected with a culture dish scribing component, a first sliding groove and a second sliding groove which are perpendicular to each other and crossed are formed in the lower surface of the supporting plate, a first sliding block and a second sliding block which are connected with the supporting plate in a sliding mode are respectively arranged on the inner sides of the first sliding groove and the second sliding groove, the first sliding block is fixedly connected with the first base, the second sliding block is fixedly connected with the second base, a connecting block is connected to the outer surfaces of the first sliding block and the second sliding block in a rotating mode, a driving block is connected to the outer surface of the connecting block in a rotating mode, one end of the driving block is connected with a motor, the motor is fixedly connected to the bottom end inside the box body.

The principle and the effect of the scheme are as follows: microorganisms needing separation and purification are placed in the culture dish scribing component, then the culture dish component is placed in the first base and the second base respectively, a power supply of a motor is started, an output end of the motor drives a transmission block to rotate together, the transmission block is rotationally connected with a connecting block, the connecting block is rotationally connected with a first sliding block and a second sliding block simultaneously, the first sliding block and the second sliding block slide in a first sliding groove and a second sliding groove respectively, the connecting block can automatically transmit when rotating along the axial direction of the transmission block and drives the first sliding block to reciprocate in the first sliding groove, the second sliding block reciprocates in the second sliding groove, and the first sliding block and the second sliding block can drive the culture dish scribing component to reciprocate together while reciprocating, so that the effect of uniform stirring and shaking is achieved; after the stirring is shaken evenly, the mechanism inside the culture dish scribing component is used for quickly scribing the culture dish, so that the period of microorganism separation and purification can be effectively shortened, and the working efficiency is improved.

Further, the culture dish subassembly of ruling includes culture dish, sealed lid and division board, the culture dish can be dismantled with the base and be connected, the division board slides and wears to establish in one side of sealed lid, one side that the division board is close to the inside bottom of culture dish is equipped with a plurality of fencing bars, the fixed surface of sealed lid is connected with the sleeve, the push rod is worn to be equipped with in sleeve one end slip, push rod one end and division board fixed connection, telescopic inboard is equipped with the spring, spring one end and push rod surface butt, the spring other end and the surperficial butt of sealed lid.

By adopting the scheme, after the microorganisms in the culture dish are shaken up, only the push rod needs to be pressed downwards, the spring stretches, the push rod drives the partition plate to move downwards, and the partition grid can partition the microorganisms in the culture dish to replace the purpose of manual scribing; after separation, the push rod is released, and the push rod and the separation plate can be reset under the action of the spring.

Further, the push rod is in threaded connection with the partition plate.

By adopting the scheme, the partition plate can be conveniently taken down to be cleaned and disinfected, and the next experiment is prevented from being influenced.

Further, the bottom of the culture dish is connected with an iron sheet, magnets are embedded on the upper surfaces of the first base and the second base, and the iron sheet is matched with the magnets.

By adopting the technical scheme, the iron sheet at the bottom of the culture dish is magnetically connected with the magnet on the upper surface of the base, so that the culture dish can be quickly detached.

Furthermore, one side of the top end of the box body is rotatably connected with a cover plate, and the surface of the cover plate is connected with an ultraviolet sterilizing lamp.

By adopting the technical scheme, after the cover plate and the box body are closed, the ultraviolet sterilizing lamp can sterilize and disinfect the interior of the box body.

Compared with the prior art, the utility model has the beneficial effects that:

the two culture dishes are mechanically and automatically shaken uniformly by matching the motor, the transmission block, the connecting block, the base and the like, the purpose of manual lineation is realized by the separation grid at the bottom of the separation plate, the work efficiency of separation and purification is improved, and the two culture dishes can be shaken uniformly at the same time; the division board can be pulled down and wash, and the surface of apron is connected with sterilamp, can furthest guarantee the accuracy of experiment.

Drawings

FIG. 1 is a schematic structural view of a microorganism separation and purification apparatus according to the present invention;

FIG. 2 is a bottom view of the support plate of the present invention;

FIG. 3 is an exploded view of a streaking assembly of a culture dish according to the present invention;

FIG. 4 is a schematic view of the structure of the partition plate of the present invention.

Detailed Description

The following is further detailed by the specific embodiments:

reference numerals in the drawings of the specification include: the device comprises a box body 1, a supporting plate 2, a first through hole 3, a second through hole 4, a first base 5, a second base 6, a first sliding groove 7, a second sliding groove 8, a first sliding block 9, a second sliding block 10, a connecting block 11, a transmission block 12, a culture dish 13, a sealing cover 14, a separation plate 15, a separation grid 16, a sleeve 17, a push rod 18, a cover plate 19 and an ultraviolet sterilizing lamp 20.

Example (b):

as shown in figures 1 to 4, a microorganism separation and purification device comprises a box body 1, an opening is arranged above the box body 1, a cover plate 19 is rotatably connected to the opening, an ultraviolet sterilization lamp 20 for killing bacteria in the box body 1 is fixedly connected to the inner side surface of the cover plate 19, a support plate 2 is fixedly connected to the inner wall of the box body 1, a motor fixedly connected with the bottom end in the box body 1 is arranged below the support plate 2, the motor is not shown in the figures, a first through hole 3 and a second through hole 4 which are perpendicular and crossed are arranged on the surface of the support plate 2, the intersection point of the first through hole 3 and the second through hole 4 is located in the center of the support plate 2, the first through hole 3 and the second through hole 4 are respectively connected with a first base 5 and a second base 6, the first base 5 and the second base 6 can be detachably connected with a culture dish 13 scribing component, and the culture dish 13 scribing component comprises a culture dish 13, The bottom of the culture dish 13 is adhered with an iron sheet, the patch is embedded at the bottom of the culture dish 13, magnets are embedded on the upper surfaces of the first base 5 and the second base 6, the iron sheet is matched with the magnets, the partition plate 15 is slidably arranged on the lower side of the seal cover 14 in a penetrating manner, a plurality of criss-cross separation grids 16 are arranged on one side, close to the bottom end inside the culture dish 13, of the partition plate 15, a containing cavity is defined between the adjacent separation grids 16, a sleeve 17 is fixedly connected to the surface of the seal cover 14, a push rod 18 is slidably arranged at the right end of the sleeve 17 in a penetrating manner, the left end of the push rod 18 is in threaded connection with the partition plate 15, a spring is arranged on the inner side of the sleeve 17, one end of the spring is abutted to the outer surface of the push rod 18, the other end of the spring is abutted to the surface of the seal cover 14, and the spring can enable the push rod 18 to reset after being pressed;

mutually perpendicular and criss-cross first spout 7 and second spout 8 have been seted up to 2 lower surfaces of backup pad, first spout 7 and the coincidence of first through-hole 3 each other, coincidence of second spout 8 and second through-hole 4 each other,

first spout

7 and 8 inboards of second spout are equipped with respectively with 2 sliding connection's of backup pad first slider 9 and second slider 10, fixed connection between first slider 9 upper surface and 5 bottoms of first base, fixed connection between second slider 10 upper surface and 6 bottoms of second base,

first slider

9 and 10 bottoms of second slider surface rotate jointly and are connected with connecting block 11, connecting block 11 bottom surface rotates jointly and is connected with driving block 12, driving block 12 is "L" shape, the lower extreme of driving block 12 even is connected with the output of motor.

The specific implementation process is as follows:

when the microorganism separating and purifying device is used, microorganisms needing to be separated and purified are placed in the culture dish 13, the sealing cover 14 and the culture dish 13 are covered and then placed on the inner side of any base, the culture dish 13 can be placed on the inner sides of the two bases at the same time, then the power supply of the motor is started, the motor is matched with the connecting block 11 and the like through the transmission block 12, manual stirring and mixing can be replaced, and the labor cost and the time cost can be greatly reduced; after shaking up, the culture dish 13 is taken down, the push rod 18 at the upper end of the sealing cover 14 is pressed, the push rod 18 can push the separation plate 15 with the separation grid 16 to move towards the bottom end of the culture dish 13, the separation grid 16 separates microbial colonies in the culture dish 13, the purpose of manual marking is replaced, the time for separating and purifying the microorganisms can be effectively shortened, and the working efficiency is improved.

The above embodiments are only examples of the present invention, and the common general knowledge of the known specific structures and characteristics in the embodiments is not described too much, it should be noted that, for those skilled in the art, it can make several variations and modifications without departing from the structure of the present invention, and these should be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. The utility model provides a little bioseparation purification device, includes box (1), its characterized in that: the inner wall of the box body (1) is fixedly connected with a supporting plate (2), the supporting plate (2) is provided with a first through hole (3) and a second through hole (4) which are perpendicular to each other and crossed, the first through hole (3) and the second through hole (4) are respectively connected with a first base (5) and a second base (6), the first base (5) and the second base (6) can be detachably connected with a culture dish (13) scribing component, the lower surface of the supporting plate (2) is provided with a first sliding groove (7) and a second sliding groove (8) which are perpendicular to each other and crossed, the inner sides of the first sliding groove (7) and the second sliding groove (8) are respectively provided with a first sliding block (9) and a second sliding block (10) which are in sliding connection with the supporting plate (2), the first sliding block (9) is fixedly connected with the first base (5), and the second sliding block (10) is fixedly connected with the second base (6), first slider (9) and second slider (10) surface rotate jointly and are connected with connecting block (11), connecting block (11) surface rotates and is connected with transmission piece (12), transmission piece (12) one end is connected with the motor, motor fixed connection is in the inside bottom of box (1).

2. The apparatus for separating and purifying microorganisms as claimed in claim 1, wherein: culture dish (13) subassembly of ruling includes culture dish (13), sealed lid (14) and division board (15), culture dish (13) can be dismantled with the base and be connected, one side at sealed lid (14) is worn to establish in division board (15) slip, one side that division board (15) are close to culture dish (13) inside bottom is equipped with a plurality of fencing bars (16), the fixed surface of sealed lid (14) is connected with sleeve (17), push rod (18) are worn to be equipped with in sleeve (17) one end slip, push rod (18) one end and division board (15) fixed connection, the inboard of sleeve (17) is equipped with the spring, spring one end and push rod (18) surface butt, the spring other end and sealed lid (14) surface butt.

3. The apparatus for separating and purifying microorganisms as claimed in claim 2, wherein: the push rod (18) is in threaded connection with the partition plate (15).

4. The apparatus for separating and purifying microorganisms as claimed in claim 3, wherein: the bottom of the culture dish (13) is connected with an iron sheet, magnets are embedded on the upper surfaces of the first base (5) and the second base (6), and the iron sheet is matched with the magnets.

5. The apparatus for separating and purifying microorganisms as claimed in claim 1, wherein: one side of the top end of the box body (1) is rotatably connected with a cover plate (19), and the surface of the cover plate (19) is connected with an ultraviolet sterilizing lamp (20).