CN216771761U

CN216771761U - Cryptococcus detection card

Info

Publication number: CN216771761U
Application number: CN202122356562.8U
Authority: CN
Inventors: 文文; 俞国庆; 赖国祥; 谷雷; 余宗阳; 尤彦菁
Original assignee: 900th Hospital of the Joint Logistics Support Force of PLA
Current assignee: 900th Hospital of the Joint Logistics Support Force of PLA
Priority date: 2021-09-28
Filing date: 2021-09-28
Publication date: 2022-06-17
Anticipated expiration: 2031-09-28

Abstract

The utility model discloses a cryptococcus detection card, which is characterized by comprising the following components: the device comprises a shell, a shell cap, a knob timer, a window, a bottom plate, a sample pad, a gold label pad, a first water absorption pad, a second water absorption pad, a chromatographic membrane, a detection line and a quality control line; according to the utility model, cryptococcus capsular polysaccharide and a colloidal gold-labeled cryptococcus antibody are combined to form a compound, the compound moves along a detection card under the action of chromatography, and reacts with the pre-coated cryptococcus antibody when passing through a detection line, the detection line is made to be red, whether the detection line is red or not is judged, the cryptococcus is detected to be positive or negative, a free gold-labeled antibody is combined with a goat anti-mouse antibody to show a red strip when a quality control line 12 is formed, whether the chromatography process is normal or not is judged according to whether the quality control line is red or not, meanwhile, the detection card is provided with a timing function, an operator is prompted to finish the detection process after the chromatography process is finished, and the detection card is safe and sanitary when in use.

Description

Cryptococcus detection card

Technical Field

The utility model belongs to the field of medical equipment, and particularly relates to a cryptococcus detection card.

Background

The cryptococcus is a circular thallus, the periphery of the cryptococcus is provided with a capsule, the cryptococcus is provided with reflective spores, 37 different species are known in the cryptococcus, but most of the cryptococcus is novel cryptococcus with pathogenicity, and the cryptococcus is characterized in that the thallus is coated by a layer of thick polysaccharide capsule; with the increase of AIDS patients, the incidence rate of various opportunistic infections is increased, cryptococcus neoformans becomes the most common opportunistic infection fungus after candida albicans, and the infection rate of the cryptococcus neoformans in HIV reaches 8.5 percent; the incidence and mortality of cryptococcal meningitis can reach 10-30% in developed countries and 50-100% in developing countries; the clinical manifestations of early cryptococcus infection lack specificity, the cerebrospinal fluid changes are atypical, but when cryptococcus meningitis develops, the death rate can reach 20%, so that early, quick and accurate diagnosis is necessary;

clinically adopted diagnostic methods comprise a latex agglutination method, an ELISA method and a colloidal gold method, and literature research reports that the detection results of the ELISA method and the colloidal gold method are highly consistent, but the sensitivity of the latex agglutination method is lower than that of the former two methods; the ELISA method has long operation time, needs professional equipment such as an enzyme-linked immunosorbent assay (ELISA) instrument and the like, is not suitable for on-site rapid detection, and is particularly not suitable for developing screening and early diagnosis in suspected infection and high risk groups; the immune colloidal gold technology is a POCT (point of care testing) diagnosis technology, colloidal gold is used as a color marker, and colloidal gold particles are negatively charged in a weak alkali environment, can be electrostatically combined with positively charged genes such as protein molecules and the like, and do not influence the biological characteristics of the genes, so that the colloidal gold particles can be specifically combined with objects to be detected (antibodies, antigens, small molecules and the like) to realize the rapid diagnosis of the corresponding molecules to be detected; particularly, the colloidal gold reagent has low production cost and detection cost, is particularly suitable for screening suspected people, and can greatly reduce the screening cost and the clinical death rate; is particularly suitable for screening and early diagnosis in suspected infection and high risk groups.

SUMMERY OF THE UTILITY MODEL

The utility model provides a cryptococcus detection card, which is characterized in that cryptococcus capsular polysaccharide and a cryptococcus antibody marked by colloidal gold are combined to form a compound, the compound moves along the detection card under the action of chromatography, and reacts with the pre-coated cryptococcus antibody when passing through a detection line, the detection line is made to be red, whether the cryptococcus is detected to be positive or negative is judged by the fact that whether the detection line is red or not, a free gold marked antibody is combined with a goat anti-mouse antibody to show a red strip when a quality control line 12 is used, whether the chromatography process is normal or not is judged by the fact that the quality control line is red or not, meanwhile, the detection card is provided with a timing function, an operator is prompted after the chromatography process is finished, and the detection card is safe and sanitary when in use.

The technical scheme adopted by the utility model is that the cryptococcus detection card comprises the following components: the device comprises a shell, a shell cap, a knob timer, a window, a bottom plate, a sample pad, a gold label pad, a first water absorption pad, a second water absorption pad, a chromatographic membrane, a detection line and a quality control line; the front end of the shell is connected with a detachable shell cap, the upper end surface of the rear end of the shell is connected with a knob timer, and meanwhile, the middle of the top surface of the shell is provided with a window; the detection card is formed by connecting the bottom plate, the sample pad, the gold label pad, the first water absorption pad, the second water absorption pad, the chromatographic membrane, the detection line and the quality control line, the sample pad, the gold label pad, the first water absorption pad and the second water absorption pad are sequentially lapped on the upper end surface of the bottom plate from front to back, the chromatographic membrane is connected to the upper end surface of the first water absorption pad, and the detection line and the quality control line are arranged on the chromatographic membrane from front to back; the bottom plate and the sample pad of the detection card are exposed out of one section from the front end of the shell, the rest parts are arranged in the shell, and the detection line and the quality control line on the chromatographic membrane are exposed in the window;

further, the sample pad and the gold label pad are treated by adopting a pretreatment buffer solution, the pretreatment buffer solution contains at least one of glycinate acid solution, Tris-Cl buffer solution, borate buffer solution, phosphate buffer solution and citric acid buffer solution, and the pretreatment solution also contains a surfactant;

further, a first antibody of colloidal gold labeled cryptococcus resistant capsular polysaccharide is adsorbed on the gold labeled pad;

further, the detection line is coated with a second monoclonal antibody of capsular polysaccharide of cryptococcus;

furthermore, the quality control line is coated with goat anti-mouse IgG polyclonal antibody.

The utility model has the beneficial effects that:

1. the cryptococcus detection card enables cryptococcus capsular polysaccharide and cryptococcus antibody marked by colloidal gold to be combined to form a compound, the compound moves along the detection card under the action of chromatography, and reacts with the pre-coated cryptococcus antibody when passing through a detection line, the detection line is made to be red, whether the cryptococcus detection is positive or negative is judged through the fact that whether the detection line is red or not, a red strip is shown through the fact that free gold marked antibody is combined with goat anti-mouse antibody when a quality control line 12 is formed, and whether the chromatography process is normal or not is judged through the fact that whether the quality control line is red or not;

2. the cryptococcus detecting card has a timing function, can be used for screwing a timer when the detection is started, and prompts an operator to finish the detection process after the chromatography process is finished;

3. the cryptococcus detection card is safe and sanitary when in use, and operators can not contact detection samples all the time in the detection process due to the existence of the shell and the shell cap.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a block diagram of the present invention;

FIG. 2 is a block diagram of the test card of the present invention;

in the drawings, the components represented by the respective reference numerals are listed below:

1-shell, 2-shell cap, 3-knob timer, 4-window, 5-bottom plate, 6-sample pad, 7-gold label pad, 8-first water absorption pad, 9-second water absorption pad, 10-chromatographic membrane, 11-detection line and 12-quality control line.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

Referring to fig. 1 to 2, a structure of a cryptococcus detecting card includes: the device comprises a shell 1, a shell cap 2, a knob timer 3, a window 4, a bottom plate 5, a sample pad 6, a gold label pad 7, a first water absorption pad 8, a second water absorption pad 9, a chromatographic membrane 10, a detection line 11 and a quality control line 12; the front end of the shell 1 is connected with a detachable shell cap 2, the upper end face of the rear end of the shell 1 is connected with a knob timer 3, and meanwhile, the middle of the top face of the shell 1 is provided with a window 4; the detection card is formed by connecting the bottom plate 5, the sample pad 6, the gold label pad 7, the first water absorption pad 8, the second water absorption pad 9, the chromatographic membrane 10, the detection line 11 and the quality control line 12, the sample pad 6, the gold label pad 7, the first water absorption pad 8 and the second water absorption pad 9 are sequentially overlapped on the upper end surface of the bottom plate 1 from front to back, the chromatographic membrane 10 is connected to the upper end surface of the first water absorption pad 8, and the detection line 11 and the quality control line 12 are arranged on the chromatographic membrane 10 from front to back; the bottom plate 5 and the sample pad 6 of the detection card are exposed out of one section from the front end of the shell 1, the rest part is in the shell 1, and the detection line 11 and the quality control line 12 on the chromatographic film 10 are exposed in the window 4; the existence of the shell 1 can prevent an operator from contacting the detection card and the detection sample in the whole detection process; the shell cap 2 protects the detection card from being polluted before use; the knob timer 3 can count time after starting detection; the window 4 is used for observing whether the detection line 11 and the quality control line 12 are colored or not;

the sample pad 6 and the gold label pad 7 are treated by adopting a pretreatment buffer solution, the pretreatment buffer solution contains at least one of glycinate acid solution, Tris-Cl buffer solution, borate buffer solution, phosphate buffer solution and citric acid buffer solution, and the pretreatment solution also contains a surfactant, so that the combination of cryptococcus antibodies and colloidal gold particles is more stable, and the detection sensitivity can be improved;

the detection line 11 is coated with a second monoclonal antibody of capsular polysaccharide of cryptococcus, and whether the chromatography process is normal is judged through whether the detection line 11 is colored;

the quality control line 12 is coated with goat anti-mouse IgG polyclonal antibody, the mouse antibody is the most common antibody form in the current commercial antibodies, the source is wide, the cost is low, the specificity and the stability are good, and the detection result is positive or negative according to the color development of the detection line 12.

The operation principle and the specific use method are as follows:

firstly, taking down a shell cap 2 on a shell 1, immersing a detection card exposed out of the shell 1 into a detection sample, taking out the detection card after soaking a sample pad, and flatly placing the detection card on a table to start detection; secondly, a knob timer 3 on the shell 1 is screwed, the knob timer 3 is reset to an original state after 5min, and an operator is reminded of the end of the detection process by sound; thirdly, judging the detection result by observing the quantity of the red strips appearing in the window 4 on the shell 1; finally, in the chromatography process, the cryptococcus capsular polysaccharide is combined with the colloidal gold-labeled cryptococcus antibody to form a compound, the compound moves to the second water absorption pad 9 along the sample pad 6 of the detection card under the chromatography action, and reacts with the pre-coated cryptococcus antibody when passing through the detection line 11 to form an immune compound and present a red strip, and the free gold-labeled antibody is combined with the goat anti-mouse antibody and present a red strip when passing through the quality control line 12; if the detection sample is negative, cryptococcus capsular polysaccharide is not contained, immune complex cannot be formed, no strip appears at the detection line 11, and only the quality control line 12 develops color; the quality control line 12 should have a band when detecting both the positive sample and the negative sample, and the red band is the standard for determining whether the chromatography process is normal.

In summary, in the cryptococcus detecting card 1, cryptococcus capsular polysaccharide and the colloidal gold-labeled cryptococcus antibody are combined to form a compound, the compound moves along the detecting card under the action of chromatography, and reacts with the pre-coated cryptococcus antibody when passing through the detecting line, the detecting line is made to be red, whether the cryptococcus is detected to be positive or negative is judged through the red color development of the detecting line, the free gold-labeled antibody is combined with the goat anti-mouse antibody to display a red strip when the free gold-labeled antibody is on the quality control line 12, and whether the chromatography process is normal is judged through the red color development of the quality control line;

2. the cryptococcus detecting card has a timing function, can start to screw a timer when detection is started, and prompts an operator to finish the detection process after the chromatography process is finished;

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the utility model disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the utility model to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the utility model and the practical application, to thereby enable others skilled in the art to best utilize the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A cryptococcus detection card comprising: the device comprises a shell, a shell cap, a knob timer, a window, a bottom plate, a sample pad, a gold label pad, a first water absorption pad, a second water absorption pad, a chromatographic membrane, a detection line and a quality control line; the front end of the shell is connected with a detachable shell cap, the upper end surface of the rear end of the shell is connected with a knob timer, and meanwhile, the middle of the top surface of the shell is provided with a window; the detection card is formed by connecting the bottom plate, the sample pad, the gold mark pad, the first water absorption pad, the second water absorption pad, the chromatographic membrane, the detection line and the quality control line, the sample pad, the gold mark pad, the first water absorption pad and the second water absorption pad are sequentially lapped on the upper end surface of the bottom plate from front to back, the chromatographic membrane is connected to the upper end surface of the first water absorption pad, and the detection line and the quality control line are arranged on the chromatographic membrane from front to back; the bottom plate and the sample pad of the detection card are exposed out of one section from the front end of the shell, the rest part is inside the shell, and the detection line and the quality control line on the chromatographic membrane are exposed in the window.

2. The cryptococcus detection card of claim 1, wherein a colloidal gold labeled first antibody against cryptococcus capsular polysaccharide is adsorbed on the gold labeled pad.

3. The cryptococcus detection card according to claim 1, wherein the detection line is coated with a second monoclonal antibody to capsular polysaccharide of cryptococcus.

4. The cryptococcus detection card of claim 1, wherein the quality control line is coated with goat anti-mouse IgG polyclonal antibody.