CN216718454U - Colloidal gold test paper marking pen applied to aquatic animal pathogen detection - Google Patents
Colloidal gold test paper marking pen applied to aquatic animal pathogen detection Download PDFInfo
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- CN216718454U CN216718454U CN202123240145.3U CN202123240145U CN216718454U CN 216718454 U CN216718454 U CN 216718454U CN 202123240145 U CN202123240145 U CN 202123240145U CN 216718454 U CN216718454 U CN 216718454U
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- pen
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- liquid inlet
- colloidal gold
- liquid
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 238000012360 testing method Methods 0.000 title claims abstract description 24
- 238000001514 detection method Methods 0.000 title claims abstract description 17
- 244000037640 animal pathogen Species 0.000 title claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 75
- 238000003860 storage Methods 0.000 claims abstract description 15
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 238000002955 isolation Methods 0.000 claims abstract description 8
- 241001465754 Metazoa Species 0.000 claims abstract description 5
- 244000052769 pathogen Species 0.000 claims abstract description 5
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 4
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 10
- 238000002474 experimental method Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000427 antigen Substances 0.000 description 10
- 102000036639 antigens Human genes 0.000 description 10
- 108091007433 antigens Proteins 0.000 description 10
- 101000792504 Porphyra purpurea Uncharacterized protein ycf91 Proteins 0.000 description 6
- 230000009471 action Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 5
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 241001487666 Cyprinid herpesvirus Species 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- -1 F121 Species 0.000 description 1
- 229910004042 HAuCl4 Inorganic materials 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model provides a colloidal gold test paper marking pen applied to aquatic animal pathogen detection, which comprises a pen point and a pen body, wherein the pen point is screwed at the bottom end of the pen body; the pen point comprises a ball pen point and a liquid inlet pipe, the ball pen point is connected to the bottom end of the pen point, the liquid inlet pipe is fixed in the pen point, and one end of the liquid inlet pipe is close to the other end of the ball pen point to form a first liquid inlet needle; the pen body comprises a pen body, and an isolation rubber membrane, a liquid storage pipe, a one-way valve assembly and a connecting port which are arranged in the pen body from bottom to top; the first liquid inlet needle breaks the isolation rubber film part and extends into the liquid storage pipe; the connecting port is formed at the top end of the pen body and is communicated with the liquid storage tube through the one-way valve component. The colloidal gold test paper marking pen applied to the pathogen detection of the aquatic animals has the advantages of low cost, replaceable pen point, firmness and durability, experiment material saving, capability of avoiding the pollution of the experiment material and semi-quantitative effect.
Description
Technical Field
The utility model relates to the technical field of biochemical experimental instruments, in particular to a colloidal gold test paper marking pen applied to detection of aquatic animal pathogens.
Background
The immune colloidal gold technology is a novel immune labeling technology which applies colloidal gold as a tracer marker to antigen and antibody. The colloidal gold is prepared by polymerizing chloroauric acid (HAuCl4) into gold particles with specific size under the action of a reducing agent, and is in a stable colloidal state under the action of static electricity, and is called colloidal gold. The colloidal gold has negative charge in weak alkali environment and can form firm combination with the positive charge group of protein molecule, and the combination is electrostatic combination, so that the biological characteristics of the protein are not influenced. The colloidal gold is used for marking antigens or antibodies of aquatic animal pathogens, the antigens or antibodies flow towards the water absorption pad under the capillary action of the nitrocellulose membrane, aggregation can occur after the antigens or antibodies meet corresponding antibodies of the detection line and the quality control line, and the gold-marked particles are aggregated at the position to generate an optical effect, so that the detection line and the quality control line show colors.
In experiments and trial productions of laboratories or medical enterprises in colleges and universities, due to the fact that the expenditure is limited, the amount of purchased and used antigens and antibodies is small, preparation of experimental materials is difficult to carry out by using a film spraying instrument or a colloidal gold scribing instrument, waste is caused to the experimental materials, dosage specifications used in each experiment are different, the preparation process of the test paper is very complicated, misoperation is easy to cause, and the test paper is difficult to be used for small-amount preparation.
In the existing-stage colloidal gold scribing method, a gun head or some simple tools are generally used for scribing, the width of a colloidal gold detection line is controlled by nesting different gun heads, the operation is unstable and inconvenient, artificial errors can be caused in an experimental result, a cellulose nitrate film is easy to break, and waste of experimental materials and experimental failure are caused; in some labs with crude conditions, the laboratory even uses microscope coverslips to dip in colloidal gold liquid to scribe lines on nitrocellulose films.
At present, the existing scriber is provided with a colloidal gold scribing and film spraying instrument and a colloidal gold scribing pen;
wherein, the colloidal gold scribing and film spraying instrument has the following defects:
1. the price is high, and a large amount of expenses are consumed in the process of trial production of a laboratory or an enterprise.
2. Is not suitable for small-scale production or laboratory trial production, and is only suitable for large-scale batch production.
3. The parameter adjustment is complex, and the parameters need to be frequently adjusted aiming at the lineation of different antigen antibodies.
The colloidal gold marking pen has the following disadvantages:
1. the pen point and the pen point can not be replaced, and the pen point are not suitable for marking on test strips of different specifications.
2. The pen point is arc point, and it is difficult to control the flux when the antigen and antibody is drawn.
3. The bottom plugs used are susceptible to degradation resulting in loss of sealing.
4. The siphon structure can adsorb a large amount of experimental liquid and can not be discharged, leads to the waste of experimental material.
5. Because the antigen-antibody has a certain particle size, the siphon structure can be blocked after the siphon structure is used for a long time.
Disclosure of Invention
Aiming at the defects in the prior art, the utility model provides the colloidal gold test paper marking pen applied to the pathogen detection of the aquatic animals, which has the advantages of low cost, replaceable pen point, firmness and durability, experimental material saving, capability of avoiding the pollution of the experimental material and semi-quantitative effect.
In order to achieve the purpose, the utility model provides a colloidal gold test paper marking pen applied to aquatic animal pathogen detection, which comprises a pen point and a pen body, wherein the pen point is screwed at the bottom end of the pen body; the pen point comprises a ball pen point and a liquid inlet pipe, the ball pen point is connected to the bottom end of the pen point, the liquid inlet pipe is fixed in the pen point, and one end of the liquid inlet pipe is close to the other end of the ball pen point to form a first liquid inlet needle; the pen body comprises a pen body, and an isolation rubber membrane, a liquid storage pipe, a one-way valve assembly and a connecting port which are arranged in the pen body from bottom to top; the first liquid inlet needle breaks the isolation rubber membrane part and extends into the liquid storage pipe; the connecting port is formed at the top end of the pen body and is communicated with the liquid storage pipe through the one-way valve assembly.
Preferably, the disposable absorbent article further comprises an external liquid suction needle, wherein the external liquid suction needle comprises a liquid suction needle and a liquid suction needle head; the liquid suction needle is arranged in the liquid suction needle head in a penetrating way and partially extends out of the liquid suction needle head; the top of the liquid suction needle head forms a thread matched with the bottom end of the pen body; and a second liquid inlet needle is formed at the top end of the liquid suction needle.
Preferably, the check valve assembly includes a fixed orifice plate, a spring post, a spring, a valve body and a valve; the fixed orifice plate forms a plurality of through holes and is fixed below the connecting port; the spring support is fixed on the lower surface of the fixed orifice plate; the valve is fixed below the fixed orifice plate, and the valve body is tightly matched with the valve and arranged between the valve and the fixed orifice plate; the spring is sleeved on the spring support and is pressed between the fixed orifice plate and the valve body.
Due to the adoption of the technical scheme, the utility model has the following beneficial effects:
1. the cost of the colloidal gold test strip in development and pre-production is reduced, and the expenditure is saved for enterprises or laboratory subject groups.
2. The test paper can replace the ball pen points of different specifications to match, so that the experimental time is saved.
3. The size of the liquid outlet amount is controlled by replacing different ball pen points, and the ball pen points are made of metal materials, so that the ball pen points are firm and durable
4. Can absorb antigen or antibody quantitatively, and save experimental materials.
5. The check valve component enables the liquid storage pipe to be relatively sealed, avoids pollution of experimental materials, is not easy to age and is relatively reliable.
6. Has semi-quantitative effect, and is a basis for the subsequent development of accurate quantitative colloidal gold test strips.
The utility model can adopt ball pen points with various types, can be adjusted according to the requirements of colloidal gold test strips with different specifications, and can be used in laboratory experiments and enterprise preproduction; the gravity liquid outlet mode is adopted, the liquid outlet is uniform and stable, and the liquid outlet amount can be quantified; the arc contact surface of the ball pen point can not cut the nitrocellulose membrane; the external liquid suction needle can suck the antigen and antibody completely to the maximum extent, so that waste can be reduced and expenses can be saved; the used one-way valve component structure is not easy to age, is firm and durable, and can play a role in isolating and protecting antigen and antibody in the liquid storage tube; the whole manufacturing cost is low, the method can be accepted by most laboratories and enterprise research and development departments, and the market prospect is wide.
Drawings
FIG. 1 is a schematic structural diagram of a colloidal gold test paper marking pen applied to pathogen detection of aquatic animals according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of an external pipette needle according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a one-way valve assembly according to an embodiment of the present invention.
Detailed Description
The following description of the preferred embodiments of the present invention will be provided in conjunction with the accompanying drawings, which are set forth in detail below to provide a better understanding of the function and features of the utility model.
Referring to fig. 1 to 3, a colloidal gold test paper marking pen for detecting pathogens of aquatic animals according to an embodiment of the present invention includes a pen point 1 and a pen body 2, wherein the pen point 1 is screwed to a bottom end of the pen body 2; the pen point 1 comprises a ball pen point 11 and a liquid inlet pipe 12, the ball pen point 11 is connected to the bottom end of the pen point 1, the liquid inlet pipe 12 is fixed in the pen point 1, and one end of the liquid inlet pipe is close to the other end of the ball pen point 11 to form a first liquid inlet needle 13; the pen body 2 comprises a pen body, and an isolation rubber membrane 21, a liquid storage pipe 22, a one-way valve assembly 23 and a connecting port 24 which are arranged in the pen body from bottom to top; the first liquid inlet needle 13 pierces the isolation rubber film 21 and partially extends into the liquid storage tube 22; a connection port 24 is formed at the top end of the pen body 2 and communicates with the reservoir 22 through a check valve assembly 23.
The external liquid suction needle 3 comprises a liquid suction needle 31 and a liquid suction needle head; the liquid suction needle 31 is arranged in the liquid suction needle head in a penetrating way and partially extends out of the liquid suction needle head; the top of the liquid suction needle forms a thread matched with the bottom end of the pen body 2; the top end of the liquid suction needle 31 forms a second liquid inlet needle 32.
The check valve assembly 23 includes a fixed orifice plate 231, a spring support 232, a spring 233, a valve body 234, and a valve 235; the fixed orifice plate 231 is fixed below the connection port 24 by forming a plurality of through holes; the spring support posts 232 are fixed on the lower surface of the fixed orifice plate 231; the valve 235 is fixed below the fixed orifice plate 231, and the valve body 234 is tightly matched with the valve 235 and is arranged between the valve 235 and the fixed orifice plate 231; the spring 233 is sleeved on the spring support 232 and pressed between the fixed orifice 231 and the valve body 234.
When the colloidal gold test paper marking pen applied to aquatic animal pathogen detection is used, firstly, the pen head 1 is unscrewed from the pen body 2, the external liquid suction needle 3 is replaced, then, a scale-adjusted liquid transfer gun or injector is connected to the connecting port 24, the liquid suction needle 31 is immersed into the carp herpes virus ORF121 polyclonal antibody, in the process of sucking the liquid storage pipe 22, the valve body 234 is separated from the valve 235 under the action of pressure due to the unbalanced gas pressure at the two ends of the check valve assembly 23, the solution of the ORF121 polyclonal antibody enters the liquid storage pipe 22, after the liquid suction operation is completed, the check valve assembly 23 closes the check valve under the action of the elastic force of the spring 233 inside the check valve assembly 23, the liquid transfer gun or injector of the connecting port 24 is pulled down, the external liquid suction needle 3 is unscrewed and replaced into the pen head 1, the pen point 11 with the pen point of 0.5mm is selected to be installed on the pen head 1, then, the whole colloidal gold test paper is marked, the ball point pen tip 11 is lifted down to allow the solution of ORF121 polyclonal antibody to flow to the ball point pen tip 11 by gravity through the first liquid inlet needle 13 and the liquid inlet pipe 12 for later use.
The colloidal gold test paper marking pen for detecting aquatic animal pathogens is used for marking a line on a detection line on a cut nitrocellulose membrane, the colloidal gold test card for detecting the cyprinid herpesvirus is combined with other parts to form a cyprinid herpesvirus colloidal gold test card, a sample collected from a fish body is dripped on a sample pad, sample diluent is added, the sample is combined with ORF121 protein with a colloidal gold mark when reaching a colloidal gold combination pad and reaches the detection line under the capillary action of a nitrocellulose membrane, and is combined with ORF121 polyclonal antibody at the position to form a sandwich compound, and the colloidal gold is gathered and precipitated at the position to show red due to the fact that the ORF121 polyclonal antibody is adsorbed on the detection line, and the result shows that the cyprinid herpesvirus is carried by the fish.
While the present invention has been described in detail and with reference to the embodiments thereof as illustrated in the accompanying drawings, it will be apparent to one skilled in the art that various changes and modifications can be made therein. Therefore, certain details of the embodiments should not be construed as limitations of the utility model, except insofar as the following claims are interpreted to cover the utility model.
Claims (3)
1. A colloidal gold test paper marking pen applied to aquatic animal pathogen detection is characterized by comprising a pen point and a pen body, wherein the pen point is screwed at the bottom end of the pen body; the pen point comprises a ball pen point and a liquid inlet pipe, the ball pen point is connected to the bottom end of the pen point, the liquid inlet pipe is fixed in the pen point, and one end of the liquid inlet pipe is close to the other end of the ball pen point to form a first liquid inlet needle; the pen body comprises a pen body, and an isolation rubber membrane, a liquid storage pipe, a one-way valve assembly and a connecting port which are arranged in the pen body from bottom to top; the first liquid inlet needle breaks the isolation rubber membrane part and extends into the liquid storage pipe; the connecting port is formed at the top end of the pen body and is communicated with the liquid storage pipe through the one-way valve assembly.
2. The colloidal gold test paper marking pen applied to pathogen detection of aquatic animals as claimed in claim 1, further comprising an external pipette, wherein the external pipette comprises a pipette needle and a pipette tip; the liquid suction needle is connected in the liquid suction needle head in a penetrating way and partially extends out of the liquid suction needle head; the top of the liquid suction needle head forms a thread matched with the bottom end of the pen body; and a second liquid inlet needle is formed at the top end of the liquid suction needle.
3. The colloidal gold test paper streaking pen as claimed in claim 1, wherein the check valve assembly includes a fixed orifice plate, a spring support, a spring, a valve body and a valve; the fixed orifice plate forms a plurality of through holes and is fixed below the connecting port; the spring support is fixed on the lower surface of the fixed orifice plate; the valve is fixed below the fixed orifice plate, and the valve body is tightly matched with the valve and arranged between the valve and the fixed orifice plate; the spring is sleeved on the spring support and is pressed between the fixed orifice plate and the valve body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123240145.3U CN216718454U (en) | 2021-12-22 | 2021-12-22 | Colloidal gold test paper marking pen applied to aquatic animal pathogen detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123240145.3U CN216718454U (en) | 2021-12-22 | 2021-12-22 | Colloidal gold test paper marking pen applied to aquatic animal pathogen detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216718454U true CN216718454U (en) | 2022-06-10 |
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ID=81885234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123240145.3U Active CN216718454U (en) | 2021-12-22 | 2021-12-22 | Colloidal gold test paper marking pen applied to aquatic animal pathogen detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216718454U (en) |
-
2021
- 2021-12-22 CN CN202123240145.3U patent/CN216718454U/en active Active
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| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240322 Address after: Room 118, building 20, no.1-42, Lane 83, Hongxiang North Road, Lingang New District, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai, 2013 13 Patentee after: Shanghai Peimigou Network Technology Co.,Ltd. Country or region after: China Address before: 201306, No. 999, Huan Cheng Road, Pudong New Area, Shanghai Patentee before: SHANGHAI OCEAN University Country or region before: China |