CN216639514U

CN216639514U - Mesenchymal stem cell and endothelial cell co-culture device

Info

Publication number: CN216639514U
Application number: CN202122064487.8U
Authority: CN
Inventors: 张庆宇
Original assignee: Shandong Provincial Hospital Affiliated to Shandong First Medical University
Current assignee: Shandong Provincial Hospital Affiliated to Shandong First Medical University
Priority date: 2021-08-30
Filing date: 2021-08-30
Publication date: 2022-05-31
Anticipated expiration: 2031-08-30

Abstract

The utility model relates to the technical field of a mesenchymal stem cell and endothelial cell co-culture device, and discloses a mesenchymal stem cell and endothelial cell co-culture device, which comprises a culture dish body and a bottom plate, wherein the bottom plate is fixedly connected with the culture dish body, the culture dish body is provided with a region division mechanism, the region division mechanism comprises a rotating shaft, a partition plate and an alternating flow hole, the rotating shaft is rotatably connected with the culture dish body, the partition plate is rotatably connected with the rotating shaft, the side wall of the partition plate is provided with the alternating flow hole, the conventional integral culture dish body is divided into two parts by the partition plate, the surface of the culture dish body is divided into two parts by the two partition plates, cells are cultured on the two parts of the surface of the culture dish body by different culture media, the same cell is cultured by different colloidal media in the same culture dish, the control variable of an experiment is more facilitated, and the flexibility during the experiment is enhanced, and multiple groups of experiments can be performed in parallel, and the experimental result is more accurate.

Description

Mesenchymal stem cell and endothelial cell co-culture device

Technical Field

The utility model relates to the technical field of a mesenchymal stem cell and endothelial cell co-culture device, in particular to a mesenchymal stem cell and endothelial cell co-culture device.

Background

The prior art discloses cell culture dishes (CN 201620544718.1) comprising: the cell of treating the separation is cultivateed to the culture dish hole, and the cover bucket is arranged the culture dish hole in when extracting the cell in, and it is regional to circle the cell extraction of target location, and the cover bucket has three kinds, is respectively: the device comprises a first set of barrel, a second set of barrel and a third set of barrel; the first sleeve barrel is internally provided with a first sampling hole which is concentric with the first sleeve barrel; the second sleeve barrel is internally provided with a second sampling hole which is deviated from the circle center of the second sleeve barrel; the third barrel set is provided with a third sampling hole which is positioned at the edge of the barrel wall of the third barrel set. The cell culture dish is provided with three types of set barrels, the sampling hole in each set barrel is different in position so as to cover target cells at different positions in the hole of the culture dish, so that the sampling purpose is improved, the existing culture dish is single, and the bazao partition on the surface can only be used for cell culture by using one medium, so that a device for co-culturing mesenchymal stem cells and endothelial cells is urgently needed to be provided.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

Aiming at the defects of the prior art, the utility model provides a mesenchymal stem cell and endothelial cell co-culture device.

(II) technical scheme

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a common culture apparatus of mesenchymal stem cell and endothelial cell, includes culture dish body and bottom plate, bottom plate and culture dish body fixed connection, culture dish body set up regional division mechanism, regional division mechanism includes pivot, space bar and exchanges the hole, the pivot is connected with culture dish body rotation, and the space bar rotates with the pivot to be connected, and the crossing hole is seted up to the lateral wall of space bar.

Preferably, the division board is the arc board that the horizontal cross-section is the C font, and the left end face of division board rotates with the periphery of pivot to be connected, and the quantity of division board is two and two division boards all rotate with the pivot to be connected, and the periphery of division board is seted up and is exchanged the hole and exchange the hole and run through the division board.

Preferably, regional division mechanism still includes partition panel, bolt and lower spout, the bolt runs through partition panel and bolt and partition panel threaded connection, and the top surface of partition panel is opened and is established down the spout, the partition panel is located down in the spout and partition panel and lower spout swing joint, and the partition panel will be handed over the discharge orifice and cut off.

Preferably, the lower chute is arc-shaped and concentric with the partition plate, the partition plate is matched with the lower chute, and the partition plate is connected with the lower chute in a sliding manner.

Preferably, the area division mechanism further comprises a first vertical groove, a rubber block and a spring, the first vertical groove is formed in the top surface of the partition plate, the rubber block is in sliding connection with the first vertical groove, the spring is located in the first vertical groove, and the spring is simultaneously fixedly connected with the rubber block and the partition plate.

Preferably, the block rubber is bow-shaped piece and block rubber and erects a groove adaptation, block rubber and partition panel laminating, the one end of spring and the inner wall fixed connection of partition panel and the other end of spring and the lateral wall fixed connection of block rubber.

Preferably, the community that two space bars are constituteed is the S shape, and the outer convex surface of two space bars all is tangent with the edge of culture dish body, sets up the same bolt on two space bars.

(III) advantageous effects

Compared with the prior art, the utility model provides a mesenchymal stem cell and endothelial cell co-culture device, which has the following beneficial effects:

1. this mesenchymal stem cell and endothelial cell culture apparatus jointly, divide into two through the monoblock for the culture dish body that will have now, two space bars are with culture dish body surface partition two parts, culture dish body surface two parts respectively with the culture solution culture cell of difference, realize cultivateing same kind of cell with different colloidal media in same culture dish, more are favorable to the controlled variable of experiment, flexibility during the reinforcing experiment, can carry out the multiunit experiment in parallel, the experimental result is more accurate.

2. This mesenchymal stem cell and endothelial cell co-culture device through will rotating the spacer plate, the spacer plate is around the certain angle of rotation of axis of rotation, the rotatory bolt again, and the bottom surface fixed rubber layer of bolt utilizes the frictional force restriction spacer plate position at culture dish body top surface between bolt bottom surface and the culture dish body, adjusts two spacer plates to the size of culture dish body top surface area of dividing, flexibility and usability among the reinforcing experimentation.

3. This mesenchymal stem cell and endothelial cell culture apparatus jointly, through stirring the block rubber, the block rubber slides and compression spring in erecting groove one, block rubber and partition panel separation, upwards stimulate the partition panel and make the partition panel move to the top of handing-over hole, and the cell of partition panel both sides can exchange through the handing-over hole, has expanded cell culture's research field, realizes the external joint culture of two kinds of different cells.

4. This mesenchymal stem cell and endothelial cell culture apparatus jointly, through setting up the block rubber, when the block rubber is located spout below, the space bar both sides are isolated relatively, can cultivate two kinds of cells alone in the both sides of two space bars, simultaneously, also can cultivate the mixed liquid of two kinds of cells in the both sides of two space bars, and adjustable parameter is more.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is an enlarged view of A of FIG. 1 according to the present invention;

FIG. 3 is a schematic perspective view of the partition plate according to the present invention;

fig. 4 is a schematic perspective view of the partition plate of the present invention.

In the figure: 10. a culture dish body; 11. a base plate; 12. a rotating shaft; 13. a partition plate; 14. an alternating current hole; 15. a partition panel; 16. a first vertical groove; 17. a rubber block; 18. a spring; 19. a bolt; 20. a lower chute.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

Referring to fig. 1-4, a mesenchymal stem cell and endothelial cell co-culture apparatus comprises a culture dish body 10 and a bottom plate 11, wherein the bottom plate 11 is fixedly connected with the culture dish body 10, the culture dish body 10 is provided with a region division mechanism, the region division mechanism comprises a rotating shaft 12, a partition plate 13 and an alternating flow hole 14, the rotating shaft 12 is rotatably connected with the culture dish body 10, the partition plate 13 is rotatably connected with the rotating shaft 12, the alternating flow hole 14 is formed in the side wall of the partition plate 13, the existing whole culture dish body 10 is divided into two parts by the partition plate 13, the surface of the culture dish body 10 is divided into two parts by the two partition plates 13, cells are cultured by different culture solutions on the two parts of the surface of the culture dish body 10, the same cell is cultured by different colloidal media in the same culture dish, the control variable of an experiment is facilitated, and the flexibility of the experiment is enhanced, and multiple groups of experiments can be performed in parallel, and the experimental result is more accurate.

The partition plate 13 is an arc plate with a C-shaped horizontal cross section, the left end face of the partition plate 13 is rotationally connected with the circumferential face of the rotating shaft 12, the number of the partition plates 13 is two, two partition plates 13 are rotationally connected with the rotating shaft 12, the circumferential face of each partition plate 13 is provided with an alternating current hole 14, each alternating current hole 14 penetrates through each partition plate 13, the partition plate 15 further comprises a partition plate 15, a bolt 19 and a lower chute 20, each bolt 19 penetrates through each partition plate 13, each bolt 19 is in threaded connection with each partition plate 13, the top face of each partition plate 13 is provided with the lower chute 20, each partition plate 15 is positioned in each lower chute 20, each partition plate 15 is movably connected with each lower chute 20, each partition plate 15 partitions each alternating current hole 14, each lower chute 20 is an arc-shaped chute and is concentric with each partition plate 13, each lower chute 15 is matched with each partition plate 20, each lower chute 15 is slidably connected with each partition plate 20, each area dividing mechanism further comprises a first vertical chute 16, a rubber block 17 and a spring 18, the top surface of the partition plate 13 is provided with a vertical groove I16, a rubber block 17 is in sliding connection with the vertical groove I16, a spring 18 is positioned in the vertical groove I16, the spring 18 is fixedly connected with the rubber block 17 and the partition plate 13 at the same time, the rubber block 17 is an arched block, the rubber block 17 is matched with the vertical groove I16, the rubber block 17 is attached to the partition plate 15, one end of the spring 18 is fixedly connected with the inner wall of the partition plate 13, the other end of the spring 18 is fixedly connected with the side wall of the rubber block 17, the community formed by the two partition plates 13 is S-shaped, the outer convex surfaces of the two partition plates 13 are tangent to the edge of the culture dish body 10, the two partition plates 13 are provided with the same bolt 19, the position of the partition plate 13 on the top surface of the culture dish body 10 is limited by the friction force between the bottom surface of the bolt 19 and the culture dish body 10 through rotating the partition plate 13 by rotating a certain angle around the rotating shaft 12 and then rotating the bolt 19 and fixing the rubber layer on the bottom surface of the bolt 19, the division area of the two partition plates 13 on the top surface of the culture dish body 10 is adjusted, and the flexibility and the usability in the experiment process are enhanced.

Through stirring rubber block 17, rubber block 17 slides and compression spring 18 in vertical slot one 16, and rubber block 17 and partition panel 15 separation are upwards stimulateed partition panel 15 and are made partition panel 15 move to the top of handing-over hole 14, and the cell of partition panel 13 both sides can exchange through handing-over hole 14, has expanded cell culture's research field, realizes the external joint culture of two kinds of different cells.

Through setting up block rubber 17, when block rubber 17 was located spout 20 below, the space bar 13 both sides were isolated relatively, can cultivate two kinds of cells alone in the both sides of two space bars 13, simultaneously, also can cultivate the mixed liquid of two kinds of cells in the both sides of two space bars 13, and adjustable parameter is more.

When the culture dish is used, the spacing plate 13 is rotated, the spacing plate 13 rotates for a certain angle around the rotating shaft 12, the bolt 19 is rotated again, the rubber layer is fixed on the bottom surface of the bolt 19, the position of the spacing plate 13 on the top surface of the culture dish body 10 is limited by the friction force between the bottom surface of the bolt 19 and the culture dish body 10, the rubber block 17 is shifted, the rubber block 17 slides in the first vertical groove 16 and compresses the spring 18, the rubber block 17 is separated from the partition plate 15, and the partition plate 15 is pulled upwards to enable the partition plate 15 to move to the position above the cross-flow hole 14.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a mesenchymal stem cell and endothelial cell culture apparatus jointly, includes culture dish body (10) and bottom plate (11), bottom plate (11) and culture dish body (10) fixed connection, its characterized in that: culture dish body (10) set up regional division mechanism, regional division mechanism includes pivot (12), space bar (13) and exchanges hole (14), pivot (12) are rotated with culture dish body (10) and are connected, and space bar (13) are rotated with pivot (12) and are connected, and exchange hole (14) are seted up to the lateral wall of space bar (13).

2. The mesenchymal stem cell and endothelial cell co-culture device of claim 1, wherein: the division board (13) are the arc board that the horizontal cross-section is the C font, and the left end face of division board (13) rotates with the periphery of pivot (12) to be connected, and the quantity of division board (13) is two and two division boards (13) all rotate with pivot (12) to be connected, and alternating current hole (14) run through division board (13) are seted up to the periphery of division board (13).

3. The mesenchymal stem cell and endothelial cell co-culture device of claim 2, wherein: regional division mechanism still includes partition panel (15), bolt (19) and lower spout (20), bolt (19) run through partition panel (13) and bolt (19) and partition panel (13) threaded connection, and spout (20) are down established to the top surface of partition panel (13), partition panel (15) are located down spout (20) and partition panel (15) and lower spout (20) swing joint, and partition panel (15) will be handed over discharge orifice (14) and cut off.

4. The mesenchymal stem cell and endothelial cell co-culture device of claim 3, wherein: lower spout (20) are the arc wall and down spout (20) and space bar (13) concentric, partition panel (15) and lower spout (20) adaptation and partition panel (15) and lower spout (20) sliding connection.

5. The mesenchymal stem cell and endothelial cell co-culture device of claim 4, wherein: the region dividing mechanism further comprises a first vertical groove (16), a rubber block (17) and a spring (18), the first vertical groove (16) is formed in the top surface of the partition plate (13), the rubber block (17) is in sliding connection with the first vertical groove (16), the spring (18) is located in the first vertical groove (16), and the spring (18) is simultaneously fixedly connected with the rubber block (17) and the partition plate (13).

6. The mesenchymal stem cell and endothelial cell co-culture device of claim 5, wherein: the rubber block (17) is bow-shaped block and the rubber block (17) is matched with the vertical groove I (16), the rubber block (17) is attached to the partition plate (15), one end of the spring (18) is fixedly connected with the inner wall of the partition plate (13), and the other end of the spring (18) is fixedly connected with the side wall of the rubber block (17).

7. The mesenchymal stem cell and endothelial cell co-culture device of claim 6, wherein: the community that two space bars (13) are constituteed is the S shape, and the outer convex surface of two space bars (13) all is tangent with the edge of culture dish body (10), sets up same bolt (19) on two space bars (13).