CN216621921U

CN216621921U - A film-making device for traditional chinese medicine microscopic examination

Info

Publication number: CN216621921U
Application number: CN202220012160.8U
Authority: CN
Inventors: 赵晶; 姚大鹏; 郭龙伟; 麻思宇; 蒋辉; 赵翙; 李思诗
Original assignee: Individual
Current assignee: Dandong Pharmaceutical Group Co ltd
Priority date: 2022-01-05
Filing date: 2022-01-05
Publication date: 2022-05-27
Anticipated expiration: 2032-01-05

Abstract

The utility model discloses a flaking device for traditional Chinese medicine microscopic observation, which comprises an ultrasonic water bath component, a waste gas discharge component, a slide bubble discharge component and a slide placing component, wherein the ultrasonic water bath component is communicated with the waste gas discharge component and comprises a mounting groove which is arranged on the top surface of a base, an electric heating plate is fixedly arranged at the bottom of the mounting groove, a heating barrel is arranged at the top of the electric heating plate, a test tube rack is arranged at the bottom of the heating barrel, an annular ultrasonic generator is sleeved outside the heating barrel, and the side wall of the heating barrel is communicated with the waste gas discharge component. In addition, waste gas in the heating barrel is pumped out, and the safety of the test space is enhanced.

Description

A film-making device for traditional chinese medicine microscopic examination

Technical Field

The utility model relates to the technical field of ultrasonic and heating equipment, in particular to a flaking device for traditional Chinese medicine microscopic observation.

Background

In the microscopic identification process of traditional Chinese medicines, various reagents such as sealing liquid, staining solution and the like are commonly used for microscopic flaking, the time consumption is long and the efficiency is low by depending on natural permeation, bubbles are easy to generate and are not easy to discharge, and the microscopic observation effect is influenced.

The filling uses chloral hydrate to penetrate and heat substances such as starch grains, chlorophyll, resin, protein and the like in the soluble powder, so that the cells are expanded and transparent, and the tissue cell morphology can be observed conveniently. The traditional permeabilization method is to drop chloral hydrate onto microscopic powder, tissue slices and surface slices of a glass slide, and then burn and heat with an alcohol lamp flame. The operation only depends on the subjective visual inspection of an operator, and the burning degree is difficult to accurately grasp, so that the time and the labor are often consumed for repeated operation due to incomplete permeabilization, or the ideal permeabilization effect is difficult to achieve due to excessive permeabilization, black glass and bubble interference observation. And on one hand, fire hazard exists when the alcohol burner is operated by open fire, and on the other hand, chloral hydrate quickly generates carcinogenic toxic chloride due to high-temperature burning, so that the health of operators is directly harmed, and the environment is polluted.

In addition, in the pretreatment stage process of microtabletting, for different textures of the traditional Chinese medicinal materials, softening treatment needs to be carried out by adopting methods such as steaming, boiling and soaking, the number of related equipment containers is large, the experimental process is alternated back and forth, experimental errors are easy to occur, and the working efficiency is reduced.

How to overcome the defects becomes a key problem to be solved in the microscopic slice operation, and therefore, a microscopic slice device integrating multiple functions, high efficiency, controllability, safety and environmental protection needs to be designed urgently.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a flaking device for traditional Chinese medicine microscopic observation, which aims to solve or improve at least one of the technical problems, realize step integration of experimental operation and improve the working efficiency.

In order to achieve the purpose, the utility model provides the following scheme: the utility model provides a slide making device for traditional Chinese medicine microscopic observation, which comprises a base, wherein an ultrasonic water bath component, a waste gas discharge component, a slide bubble discharge component and a slide placing component are arranged in the base, the ultrasonic water bath component is communicated with the waste gas discharge component, a controller is arranged outside the base, and the ultrasonic water bath component, the waste gas discharge component and the slide bubble discharge component are respectively and electrically connected with the controller;

the ultrasonic water bath component comprises a mounting groove, the mounting groove is formed in the top surface of the base, an electric heating plate is fixedly mounted at the bottom of the mounting groove, a heating barrel is placed at the top of the electric heating plate, a test tube rack is placed at the bottom of the heating barrel, an annular ultrasonic generator is sleeved outside the heating barrel, a barrel cover is hinged to the top of the heating barrel, the side wall of the heating barrel is communicated with the waste gas discharge component, and the electric heating plate and the annular ultrasonic generator are respectively electrically connected with the controller.

Preferably, the exhaust gas component comprises an exhaust pipe, the exhaust pipe is fixedly connected and communicated with the side wall of the heating barrel, an exhaust fan is fixedly mounted on the exhaust pipe, a condensing pipe is fixedly connected and communicated with the exhaust pipe, a liquid collecting barrel is fixedly connected and communicated with the condensing pipe, the liquid collecting barrel, the exhaust pipe and the condensing pipe are all embedded in the base, and the exhaust fan is electrically connected with the controller.

Preferably, a liquid discharge pipe is fixedly mounted at the bottom of the side wall of the liquid collecting barrel, the liquid discharge pipe penetrates through the side wall of the base and extends to the outside, and a valve is fixedly mounted on the liquid discharge pipe.

Preferably, the glass slide bubble removing assembly comprises a water tank, the water tank is arranged on the top surface of the base, a first glass slide placing frame is placed in the water tank, an ultrasonic generator is installed at the bottom of the water tank and located below the first glass slide placing frame, and the ultrasonic generator is electrically connected with the controller.

Preferably, the slide placing assembly comprises a placing groove, the placing groove is formed in the top surface of the base, a second slide placing frame is placed in the placing groove, and one side of the placing groove is rotatably connected with a dust cover through a rotating shaft.

Preferably, the heating barrel is fixedly provided with a limiting ring inside, the limiting ring is located above the test tube rack, and a food steamer is placed on the limiting ring.

Preferably, a plurality of temperature sensors are fixedly mounted on the inner wall of the heating barrel and electrically connected with the controller.

Preferably, a buckle is fixedly arranged between the barrel cover and the heating barrel.

The utility model discloses the following technical effects: when the device is used, water is placed into the heating barrel, the temperature value and the heating time of the electric heating plate are set through the controller, the electric heating plate is started to heat, then traditional Chinese medicine powder or tissue slices are placed into the test tube filled with permeabilizing agents such as chloral hydrate and the like, the test tube is placed into the test tube rack, the barrel cover is closed to carry out heating and permeabilization, closed water bath heating is carried out, heating is more uniform, meanwhile, the waste gas discharging assembly is adopted to extract hot gas in the heating barrel and discharge the hot gas into the waste gas discharging assembly, test safety is enhanced spatially, and chloride is prevented from being scattered to cause harm to human bodies.

The ultrasonic cavitation infiltration increasing device is provided with the annular ultrasonic generator, and the frequency value is set through the controller, so that the ultrasonic cavitation infiltration increasing device can be independently started to perform ultrasonic cavitation infiltration increasing. On one hand, when chloral hydrate is used for heating and permeabilizing, the uniform dispersion of powder is promoted, the permeabilization speed is increased, and the generation of bubbles is reduced; on the other hand, when special tissues such as starch grains, inulin, chloroplast and the like are observed and heating and permeabilization are not needed, ultrasonic waves are used for effectively accelerating the permeation speed of the occlusion agent, and the method can also be used for permeation-increasing dyeing, dissociation of dissociation solution, bleaching treatment and the like of dyeing solution for preparing other reagents.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is an external structural view of a flaking device used for microscopic observation of traditional Chinese medicine;

FIG. 2 is a schematic view of the internal structure of a flaking device used for microscopic observation of traditional Chinese medicine;

FIG. 3 is an enlarged partial view of portion A of FIG. 2;

FIG. 4 is a top view of the flaking device for microscopic observation of traditional Chinese medicine in an uncapped state;

in the figure: 1. a base; 2. an electrical heating plate; 3. a heating barrel; 4. a test tube rack; 5. an annular ultrasonic generator; 6. a controller; 7. a barrel cover; 8. an exhaust pipe; 9. an exhaust fan; 10. a condenser tube; 11. a liquid collecting barrel; 12. a water tank; 13. a first slide placing frame; 14. an ultrasonic generator; 15. a placement groove; 16. a second slide placing frame; 17. a dust cover; 18. a liquid discharge pipe; 19. a limiting ring; 20. a food steamer; 21. a temperature sensor.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

The utility model provides a slide preparation device for traditional Chinese medicine microscopic observation, which comprises a base 1, wherein an ultrasonic water bath component, a waste gas discharge component, a slide bubble discharge component and a slide placing component are arranged in the base 1, the ultrasonic water bath component is communicated with the waste gas discharge component, a controller 6 is arranged outside the base 1, and the ultrasonic water bath component, the waste gas discharge component and the slide bubble discharge component are respectively and electrically connected with the controller 6;

ultrasonic water bath subassembly includes the mounting groove, the mounting groove is seted up in 1 top surface of base, mounting groove bottom fixed mounting has electric heating plate 2, heating barrel 3 has been placed at 2 tops of electric heating plate, test-tube rack 4 has been placed to heating barrel 3 bottoms, 3 overcoat of heating barrel are equipped with annular

supersonic generator

5, 3 top articulations of heating barrel have

bung

7, 3 lateral walls of heating barrel and exhaust gas subassembly intercommunication, electric heating plate 2, annular supersonic generator 5 respectively with 6 electric connection of controller, controller 6 can control the heating temperature and the heat time of electric heating plate 2, controller 6 also can control annular supersonic generator 5's frequency of occurrence and time of occurrence.

When the device is used, water is placed into the heating barrel 3, the set heating time and the set heating temperature of the electric heating plate 2 are set through the controller 6, then traditional Chinese medicine powder is placed into a test tube filled with chloral hydrate, the test tube is placed into the test tube rack 4, the barrel cover 7 is closed, the electric heating plate 2 is started for heating and permeabilizing, ultrasonic frequency modulation control and ultrasonic time control are set immediately, the annular ultrasonic generator 5 is started for ultrasonic cavitation, and the permeabilization speed is accelerated. According to observation of physicochemical properties of a microstructure, different sealing liquids are required to be dripped in combination with experiments, and ultrasonic wave permeation enhancement or heating and permeabilization can be independently performed by only using the annular ultrasonic generator 5 or the electric heating plate 2. The permeabilization is discharged simultaneously, the waste gas discharging assembly is opened, hot gas in the heating barrel 3 is pumped out and discharged into the waste gas discharging assembly, after gas is emptied, the barrel cover 7 is opened, the test tube is taken out, liquid in the test tube is sucked by a dropper and is dripped onto a glass slide, a cover glass is covered, if bubbles exist, the glass slide can be placed into the glass slide bubble discharging assembly for bubble removal, and after the bubbles are removed, the glass slide is placed into the glass slide placing assembly for standby. The cavitation effect, the heat effect, the mechanical and chemical alternation caused by the ultrasonic wave can generate the permeation enhancing effect, the ultrasonic wave is applied to the micro-permeabilization operation, the diffusivity and the solubility of the permeabilizing agent in a microscopic structure can be increased, the generation of bubbles is reduced, and the device can also be used for discharging the bubbles remained between the glass slide and the cover glass after the microscopic mounting.

The utility model discloses can mix the regulation and control to the permeabilization effect through the method realization of supersound and water-bath heating in putting the test tube with a small amount of permeabilization agent with micro-powder, tissue slice and surface film-making, can once prepare many loading simultaneously, improve experimental efficiency, and can effectively reduce the harm of experiment reagent toxicity material to personnel and environment.

Further optimize the scheme, the exhaust gas subassembly includes blast pipe 8,

blast pipe

8 and 3 lateral walls fixed connection of heating bucket and intercommunication, fixed mounting has air discharge fan 9 on the

blast pipe

8, 8 fixed connection of blast pipe and intercommunication have

condenser pipe

10, 10 fixed connection of condenser pipe and intercommunication have collecting bucket 11, blast pipe 8, condenser pipe 10 all inlays and locates inside the base 1,

air discharge fan

9 and 6 electric connection of controller, when needing to exhaust, start air discharge fan 9, inhale blast pipe 8 with the hot gas that contains the chloride in the heating bucket 3, then get into condenser pipe 10 condensation and become liquid, liquid flows into in collecting bucket 11 along condenser pipe 10, the chloride adsorbent has been placed at collecting bucket 11 top, adsorb the chloride wherein in the chloride adsorbent, avoid the experimentation, the operator inhales the chloride, produce the harm to the operator health.

Further optimize the scheme, 11 lateral wall bottom fixed mounting of liquid collecting bucket has fluid-discharge tube 18, and fluid-discharge tube 18 runs through the base 1 lateral wall and extends to the outside, and fixed mounting has the valve on the fluid-discharge tube 18, and when the water level was higher in the liquid collecting bucket 11, the valve can be opened, and the interior liquid of liquid collecting bucket 11 is followed fluid-discharge tube 18 and is discharged.

Further optimizing the scheme, the glass slide bubble discharging assembly comprises a water tank 12, the water tank 12 is arranged on the top surface of the base 1, a first glass slide placing frame 13 is arranged in the water tank 12, an ultrasonic generator 14 is arranged at the bottom of the water tank 12, the ultrasonic generator 14 is positioned below the first glass slide placing frame 13 and is electrically connected with the controller 6, when the glass slide bubble discharging assembly is used, a certain amount of water is injected into the water tank 12, then a glass slide is placed into the first glass slide placing frame 13, the glass slide is vertically placed in an inclined mode at 30-60 degrees, then the ultrasonic generator 14 is started, the ultrasonic generator 14 sends out ultrasonic waves, bubbles in the glass slide are vibrated away, the glass slide and a cover glass are connected and discharged, and bubbles in the glass slide are removed.

Further optimize the scheme, the subassembly is placed to the slide includes standing groove 15, and standing groove 15 is seted up in 1 top surface of base, has placed second slide rack 16 in the standing groove 15, and standing groove 15 one side is rotated through the pivot and is connected with shield 17, and the back is accomplished in the slide preparation, places the slide on second slide rack 16, stays to wait to use, when needing to place for a long time, needs to cover shield 17, avoids the dust to fall on the slide, influences the experiment.

Further optimize the scheme, fixed mounting has spacing ring 19 on heating barrel 3 inside, spacing ring 19 is located 4 tops of test-tube rack, food steamer 20 has been placed on the spacing ring 19, according to traditional chinese medicine experimental samples texture condition, can use food steamer 20 to steam and soften, place food steamer 20 on spacing ring 19, close bung 7, open electric heating board 2, heat 3 water-logging of heating barrel, make aquatic products steam fumigate traditional chinese medicine experimental samples, the follow-up experiment of being convenient for is used, do not need to install food steamer 20 under the general condition, it just needs the installation to steam and soften. According to the utility model, the food steamer 20 and the heating barrel 3 are arranged, and pre-treatment such as steaming, boiling, soaking and the like can be carried out according to different materials of traditional Chinese medicinal materials such as rhizomes, flowers and leaves, fruits and seeds and decoction pieces, so that the experimental functionality applicability is enhanced, and the working efficiency is improved.

Further optimize the scheme, fixed mounting has a plurality of temperature sensor 21 on 3 inner walls of heating bucket, and

temperature sensor

21 and 6 electric connection of controller, temperature sensor 21 detect the temperature of 3 interior waters of heating bucket, give controller 6 for, and the controller 6 of being convenient for carries out temperature control to electric heating plate 2 according to the temperature of 3 interior waters of heating bucket.

Further optimize scheme, 7 bottoms of bung are fixed with the sealing washer, and fixed mounting has the box on

bung

7, and 3 outer wall tops of heating bucket are fixed with the son and detain, and the box detains looks adaptation with the son, covers 7 backs of bung, detains the box and detains the son and detain and compress tightly, makes bung 7 and heating bucket 3 be encapsulated situation, avoids heating 3 interior chlorides effluvium.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are only intended to illustrate the preferred embodiments of the present invention, and not to limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A film-making device for traditional chinese medicine microscopic observation which characterized in that: the glass slide bubble removing device comprises a base (1), wherein an ultrasonic water bath assembly, a waste gas discharging assembly, a glass slide bubble removing assembly and a glass slide placing assembly are arranged in the base (1), the ultrasonic water bath assembly is communicated with the waste gas discharging assembly, a controller (6) is arranged outside the base (1), and the ultrasonic water bath assembly, the waste gas discharging assembly and the glass slide bubble removing assembly are respectively and electrically connected with the controller (6);

ultrasonic water bath subassembly includes the mounting groove, the mounting groove is seted up in base (1) top surface, mounting groove bottom fixed mounting has electrical heating board (2), heating bucket (3) have been placed at electrical heating board (2) top, test-tube rack (4) have been placed to heating bucket (3) bottom, heating bucket (3) overcoat is equipped with annular supersonic generator (5), heating bucket (3) top articulates there is bung (7), heating bucket (3) lateral wall with arrange waste gas subassembly intercommunication, electrical heating board (2), annular supersonic generator (5) respectively with controller (6) electric connection.

2. A flaking apparatus used in traditional Chinese medicine microscopic observation according to claim 1, characterized in that: exhaust gas subassembly includes blast pipe (8), blast pipe (8) with heating bucket (3) lateral wall fixed connection and intercommunication, fixed mounting has air discharge fan (9) on blast pipe (8), blast pipe (8) fixed connection and intercommunication have condenser pipe (10), condenser pipe (10) fixed connection and intercommunication have collecting vat (11), blast pipe (8), condenser pipe (10) all inlay and locate inside base (1), air discharge fan (9) with controller (6) electric connection.

3. A flaking apparatus used in traditional Chinese medicine microscopic observation according to claim 2, characterized in that: a liquid discharge pipe (18) is fixedly mounted at the bottom of the side wall of the liquid collecting barrel (11), the liquid discharge pipe (18) penetrates through the side wall of the base (1) and extends to the outside, and a valve is fixedly mounted on the liquid discharge pipe (18).

4. A flaking apparatus used in traditional Chinese medicine microscopic observation according to claim 1, characterized in that: the glass slide bubble removing assembly comprises a water tank (12), wherein the water tank (12) is arranged on the top surface of the base (1), a first glass slide placing frame (13) is placed in the water tank (12), an ultrasonic generator (14) is installed at the bottom of the water tank (12), the ultrasonic generator (14) is located below the first glass slide placing frame (13), and the ultrasonic generator is electrically connected with the controller (6).

5. A flaking apparatus used in traditional Chinese medicine microscopic observation according to claim 1, characterized in that: the glass slide placing assembly comprises a placing groove (15), the placing groove (15) is arranged on the top surface of the base (1), a second glass slide placing frame (16) is placed in the placing groove (15), and a dust cover (17) is rotatably connected to one side of the placing groove (15) through a rotating shaft.

6. A tabletting device for microscopic observation of traditional Chinese medicine according to claim 1, wherein: heating barrel (3) inside fixed mounting has spacing ring (19), spacing ring (19) are located test-tube rack (4) top, food steamer (20) have been placed on spacing ring (19).

7. A flaking apparatus used in traditional Chinese medicine microscopic observation according to claim 1, characterized in that: a plurality of temperature sensors (21) are fixedly mounted on the inner wall of the heating barrel (3), and the temperature sensors (21) are electrically connected with the controller (6).

8. A tabletting device for microscopic observation of traditional Chinese medicine according to claim 1, wherein: a buckle is fixedly arranged between the barrel cover (7) and the heating barrel (3).