CN216550437U

CN216550437U - Real-time fluorescent quantitative PCR device

Info

Publication number: CN216550437U
Application number: CN202123190346.7U
Authority: CN
Inventors: 白国栋; 张劲夫; 杨文军
Original assignee: Xinyi Manufacturing Technology Beijing Co ltd
Current assignee: Xinyi Manufacturing Technology Beijing Co ltd
Priority date: 2021-12-17
Filing date: 2021-12-17
Publication date: 2022-05-17
Anticipated expiration: 2031-12-17

Abstract

The utility model provides a real-time fluorescence quantitative PCR device, which comprises a plurality of single-channel fluorescence detection devices and a substrate, wherein each single-channel fluorescence detection device can be respectively and independently arranged on the substrate so as to connect the single-channel fluorescence detection devices into a whole, and each single-channel fluorescence detection device can generate exciting light and transmit a fluorescence signal excited by the exciting light to a corresponding photoelectric detection part. The utility model can realize multi-channel arbitrary splicing according to the requirements of users and projects, can adjust the distance between two adjacent single-channel fluorescence detection devices by arranging different single-channel fluorescence detection devices at different positions of the substrate, and has the advantages of richer use working conditions, more flexible arrangement and more convenient use and integration.

Description

Real-time fluorescent quantitative PCR device

Technical Field

The utility model belongs to the technical field of biological sample optical detection devices, and particularly relates to a real-time fluorescence quantitative PCR device.

Background

The real-time fluorescence quantitative PCR device (also called as a fluorescence detector) is an indispensable photoelectric detection device in the process of realizing fluorescence quantitative polymerase chain reaction (RT-PCR), and photoelectric signals of a sample in the amplification process can be collected through the device, and are processed and analyzed simultaneously. In recent years, with the development of science and technology, the weight and size of instruments and equipment have been specially required in the fields of medical equipment, and the like, so that the development of in vitro diagnostic equipment towards miniaturization and miniaturization is promoted. In the field of molecular diagnostics, different dyes are generally required to be adapted to the real-time fluorescent quantitative PCR device according to the requirements of users, fluorescent detection channels are increased or decreased, and meanwhile, the development of a real-time fluorescent quantitative PCR device with a more flexible miniaturized structure is very important and an insurmountable ring in order to reduce the volume and weight of related instruments and equipment. The applicant previously applied for a fluorescence detector, which has a plurality of single-channel fluorescence detection devices respectively provided with a shell to form random assembly, the deployment is more flexible, and the use and integration are more convenient, but the two adjacent single-channel fluorescence detection devices of the real-time fluorescence quantitative PCR device with the structure are buckled and connected through the shell, the distance between the two single-channel fluorescence detection devices cannot be flexibly adjusted, and the application working condition of the fluorescence detector is limited.

SUMMERY OF THE UTILITY MODEL

Therefore, the technical problem to be solved by the present invention is to provide a real-time fluorescence quantitative PCR device, which can realize multi-channel arbitrary splicing according to user and project requirements, and can adjust the distance between two adjacent single-channel fluorescence detection devices by arranging different single-channel fluorescence detection devices at different positions of a substrate, so that the device has the advantages of richer use conditions, more flexible deployment, and more convenient use and integration.

In order to solve the above problems, the present invention provides a real-time fluorescence quantitative PCR device, which includes a plurality of single-channel fluorescence detection devices and a substrate, wherein each single-channel fluorescence detection device can be independently mounted on the substrate to connect the single-channel fluorescence detection devices into a whole, and each single-channel fluorescence detection device can generate an excitation light and transmit a fluorescence signal excited by the excitation light to a corresponding photoelectric detection component.

In some embodiments, the real-time fluorescent quantitative PCR device is arranged below a sample to be detected, and the sample is subjected to lighting detection from the bottom.

In some embodiments, the single-channel fluorescence detection device has an outer housing, a protrusion is disposed on a side surface of the outer housing, a corresponding groove is disposed on the substrate, and the protrusion and the groove are mutually buckled and connected into a whole.

In some embodiments, the outer case includes a case body, the protrusion is on one side of the case body, the case body is configured with a light path, and the light path has an open opening, and the open opening is covered with a cover plate.

In some embodiments, the optical path channel includes a first channel and a second channel perpendicular to each other, an excitation light source, a first lens, a first optical filter and a second lens are sequentially disposed on the first channel along an emission path of the excitation light, and a third lens, a dichroic mirror, a fourth lens, a second optical filter and a photoelectric detection component are sequentially disposed on the second channel along an emission path of the fluorescent light, wherein the dichroic mirror is located at an intersection position of the first channel and the second channel, so as to reflect the excitation light emitted by the excitation light source to the third lens and allow the fluorescent signal to be transmitted to the second optical filter.

In some embodiments, the first lens, the second lens, the third lens, and the fourth lens are one of a spherical lens, a semi-spherical lens, an aspherical lens, and a cylindrical lens.

In some embodiments, the excitation light source is one of a laser diode, a light emitting diode, or an incandescent bulb; and/or the excitation wavelength of the excitation light source is between 350nm and 850 nm.

In some embodiments, the photodetecting component is one of a charge coupler, a complementary metal oxide semiconductor, a photomultiplier tube, an avalanche photodiode, a silicon photodiode; and/or the receiving wavelength of the photoelectric detection component is between 360nm and 880 nm.

The real-time fluorescent quantitative PCR device provided by the utility model can realize multi-channel arbitrary splicing according to the requirements of users and projects, and can adjust the distance between two adjacent single-channel fluorescent detection devices by arranging different single-channel fluorescent detection devices at different positions of the substrate, so that the device has the advantages of richer use conditions, more flexible deployment and more convenient use and integration.

Drawings

FIG. 1 is a schematic structural diagram (including a sample tube) of a real-time fluorescence quantitative PCR apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of an assembled structure of the single-channel fluorescence detection device in FIG. 1;

FIG. 3 is a schematic view of the disassembled structure of the single-channel fluorescence detection device in FIG. 1;

FIG. 4 is a schematic diagram of the internal principle of the single-channel fluorescence detection device in FIG. 1.

The reference numerals are represented as:

1. a single channel fluorescence detection device; 11. a box body; 111. a protrusion; 12. a cover plate; 2. a substrate; 21. a groove; 31. a first lens; 32. a first optical filter; 33. a second lens; 34. a third lens; 35. a dichroic mirror; 36. a fourth lens; 37. a second optical filter; 38. a photodetecting part; 4. an excitation light source; 5. a sample microdroplet; 6. and (4) a sample tube.

Detailed Description

Referring to fig. 1 to 4 in combination, according to an embodiment of the present invention, a real-time fluorescence quantitative PCR device is provided, which includes a plurality of single-channel fluorescence detection devices 1 and a substrate 2, each of the single-channel fluorescence detection devices may be independently mounted on the substrate 2 to connect the plurality of single-channel fluorescence detection devices 1 into a whole, and each of the single-channel fluorescence detection devices 1 may generate excitation light and may transmit a fluorescence signal excited by the excitation light to a corresponding photoelectric detection component 38. Among this technical scheme, can realize the arbitrary concatenation of multichannel according to user and project demand, can arrange the different positions department at base plate 2 through single channel fluorescence detection device 1 with the difference, make two adjacent single channel fluorescence detection device 1's interval obtain the adjustment, use operating mode is abundanter, and it is more nimble, use and integrated more convenient to deploy.

The whole real-time fluorescence quantitative PCR device is arranged below a sample to be detected, and the sample is detected from the bottom, so that the arrangement can obviously shorten the optical path of the collected optical signal, and is favorable for improving the detection sensitivity. As shown in fig. 1 in particular, as a specific embodiment, the real-time fluorescence quantitative PCR device includes six single-channel fluorescence detection devices 1, i.e. a six-channel fluorescence quantitative PCR device is formed, and under some other conditions, for example, when fewer or more channels are needed, only the number of the single-channel fluorescence detection devices 1 needs to be correspondingly reduced or increased, which is particularly convenient and has a simple and compact structure.

In some embodiments, the single-channel fluorescence detection device 1 has an outer shell, a protrusion 111 is disposed on a side surface of the outer shell, a groove 21 for alignment is disposed on the substrate 2, the protrusion 111 corresponds to the groove 21, and the single-channel fluorescence detection device 1 corresponds to the groove 21 on the substrate 2, so that the single-channel fluorescence detection device 1 can be assembled. In this technical scheme a plurality of single channel fluorescence detection device 1 is in same piece through the lock the concatenation is realized to the mode on the base plate 2, only needs to change as required base plate 2, adjustment that can be convenient the passageway number of single channel fluorescence detection device 1 through changing the interval between the recess 21 on the base plate, can change adjacent two distance between the single channel fluorescence detection device 1.

In some embodiments, the outer casing includes a case 11, the protrusion 111 is on one side of the case 11, the case 11 is configured with a light path, and the light path has an open opening, and the open opening is covered with a cover plate 12, it is understood that corresponding light path components such as lenses, dichroic mirrors, color filters, light sources, etc. are disposed in the light path, and are specifically fixed by correspondingly positioned slots of the case 11 and/or the cover plate 12.

In some embodiments, referring to fig. 3 and fig. 4 in combination, the optical path channel includes a first channel and a second channel perpendicular to each other, an excitation light source 4, a first lens 31, a first optical filter 32, and a second lens 33 are sequentially disposed on the first channel along an emission path of the excitation light, and a third lens 34, a dichroic mirror 35, a fourth lens 36, a second optical filter 37, and the photodetection component 38 are sequentially disposed on the second channel along an emission path of the fluorescence light, where the dichroic mirror 35 is located at an intersection position of the first channel and the second channel, so as to be able to emit the excitation light emitted by the excitation light source 4 to the third lens 34 and allow the fluorescence signal to pass to the second optical filter 37. Specifically, first, the excitation light source 4 emits excitation light with a desired wavelength, which sequentially passes through the first lens 31, the first filter 32, and the second lens 33, and is transmitted to the dichroic mirror 35, the dichroic mirror 35 reflects the light by 90 ° (in some embodiments, the dichroic mirror 35 may also reflect by 15 °, 30 °, 60 °, and 75 °), the reflected light is converged (focused) on the surface of the sample micro-droplet 5 through the third lens 34, at this time, the fluorescent dye of the target analyte in the sample micro-droplet 5 emits a certain fluorescent light (i.e., a fluorescent signal), the wavelength of the fluorescent light is longer than the wavelength emitted by the excitation light source 4, the fluorescent light generated by the fluorescent dye is collimated through the third lens 34, then enters the fourth lens 36 through the dichroic mirror 35, and finally the fluorescent light is converged on the surface of the photoelectric detection component 38 through the second filter 37, the photoelectric detection component 38 converts the light into a data format, and transmits the data to the corresponding processor.

The first lens 31, the second lens 33, the third lens 34, and the fourth lens 36 are one of a spherical lens, a semi-spherical lens, an aspherical lens, and a cylindrical lens.

In some embodiments, the excitation light source 4 is one of a laser diode, a light emitting diode, or an incandescent bulb; and/or the excitation wavelength of the excitation light source 4 is between 350nm and 850 nm; the photodetection component 38 is one of a Charge Coupled Device (CCD), a Complementary Metal Oxide Semiconductor (CMOS), a photomultiplier tube (PMT), an Avalanche Photodiode (APD), and a silicon Photodiode (PD); and/or the receiving wavelength of the photoelectric detection component 38 is between 360nm and 880 nm.

It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. The real-time fluorescence quantitative PCR device is characterized by comprising a plurality of single-channel fluorescence detection devices (1) and a base plate (2), wherein each single-channel fluorescence detection device (1) can be respectively and independently installed on the base plate (2) so as to connect the single-channel fluorescence detection devices (1) into a whole, and each single-channel fluorescence detection device (1) can generate exciting light and can transmit a fluorescence signal excited by the exciting light to a corresponding photoelectric detection part (38).

2. The real-time quantitative fluorescence PCR device according to claim 1, wherein the real-time quantitative fluorescence PCR device is disposed under the sample to be examined, and the sample (6) is detected by lighting from the bottom.

3. The real-time fluorescence quantitative PCR device according to claim 1, wherein the single-channel fluorescence detection device (1) has an outer housing, the side of the outer housing has a protrusion (111), the substrate (2) has a corresponding groove (21), and the protrusion (111) and the groove (21) are integrally connected with each other in a snap-fit manner.

4. The real-time fluorescent quantitative PCR device according to claim 3, wherein the outer case comprises a case (11), the protrusion (111) is on one side of the case (11), the case (11) is configured with an optical path, and the optical path has an open opening, and the open opening is covered with a cover plate (12).

5. The real-time fluorescent quantitative PCR device of claim 4, wherein the optical path channel comprises a first channel and a second channel perpendicular to each other, an excitation light source (4), a first lens (31), a first optical filter (32) and a second lens (33) are sequentially arranged on the first channel along the emitting path of the excitation light, a third lens (34), a dichroic mirror (35), a fourth lens (36), a second optical filter (37) and a photoelectric detection component (38) are sequentially arranged on the second channel along the emission path of the fluorescent light, wherein the dichroic mirror (35) is at an intersection of the first channel and the second channel, so as to be able to reflect the excitation light emitted by the excitation light source (4) at the third lens (34) and to allow the fluorescence signal to pass at the second filter (37).

6. The real-time fluorescence quantitative PCR device according to claim 5, wherein the first lens (31), the second lens (33), the third lens (34) and the fourth lens (36) are one of a spherical lens, a semi-spherical lens, an aspherical lens and a cylindrical lens.

7. The real-time fluorescent quantitative PCR device according to claim 5, wherein the excitation light source (4) is one of a laser diode, a light emitting diode or an incandescent bulb; and/or the excitation wavelength of the excitation light source (4) is between 350nm and 850 nm.

8. The real-time fluorescent quantitative PCR device according to claim 1, wherein the photodetection means (38) is one of a charge coupler, a complementary metal oxide semiconductor, a photomultiplier tube, an avalanche photodiode, a silicon photodiode; and/or the receiving wavelength of the photoelectric detection component (38) is between 360nm and 880 nm.