CN216712107U - Optical fiber coupling type portable nucleic acid amplification detector - Google Patents
Optical fiber coupling type portable nucleic acid amplification detector Download PDFInfo
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- CN216712107U CN216712107U CN202123412921.3U CN202123412921U CN216712107U CN 216712107 U CN216712107 U CN 216712107U CN 202123412921 U CN202123412921 U CN 202123412921U CN 216712107 U CN216712107 U CN 216712107U
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Abstract
The utility model discloses an optical fiber coupling type portable nucleic acid amplification detector which comprises a light source module, a sample amplification and fluorescence signal collection module, a temperature control module and a signal detection processing module, wherein the light source module, the sample amplification and fluorescence signal collection module, the temperature control module and the signal detection processing module are packaged in a case; the sample amplification and fluorescence signal collection module comprises a base and a cavity seat, wherein the cavity seat is connected to the base, and is provided with a cavity for placing an optical component and a fixed pipe sleeve.
Description
Technical Field
The utility model relates to the technical field of biological detection, in particular to an optical fiber coupling type portable nucleic acid amplification detector.
Background
Polymerase Chain Reaction (PCR) (polymerase chain reaction) can realize the nucleic acid amplification of the biomolecule to be detected, and further realize the high-efficiency, accurate and rapid detection and identification of the biomolecule with ultra-low concentration. The technology comprises three steps: DNA denaturation, quenching and extension. The detection technology is approved by the world health organization and is greatly recommended to be applied to the medical industry to identify germs, viruses and other microorganisms and major disease markers, so that early diagnosis of malignant diseases is realized. However, most of the existing detectors based on the PCR amplification technology have complex structures, complex assembly and debugging processes, high processing and manufacturing costs, too large and inconvenient to carry some of the detectors, and long detection time.
Disclosure of Invention
Aiming at the problems in the prior art, the utility model provides an optical fiber coupling type portable nucleic acid amplification detector which adopts an optical fiber output light source and a single-channel detection mode and is matched with a constant temperature control system, so that the optical fiber coupling type portable nucleic acid amplification detector is compact in structure, integrated in a modularization mode, low in cost, economical and practical.
The technical scheme adopted by the utility model is as follows: the optical fiber coupling type portable nucleic acid amplification detector comprises a light source module, a sample amplification and fluorescence signal collection module, a temperature control module and a signal detection processing module which are packaged in a case, wherein the light source module is connected with the sample amplification and fluorescence signal collection module through an optical fiber; the sample amplification and fluorescence signal collection module comprises a base and a cavity seat, the cavity seat is connected to the base, the cavity seat is provided with a cavity for placing an optical component and a fixed pipe sleeve, the side wall of the fixed pipe sleeve is provided with a light transmitting hole and a temperature measuring hole, the side wall of the fixed pipe sleeve is wound with an electric heating wire, an insulating layer is arranged between the electric heating wire and the side wall of the fixed pipe sleeve, a thermocouple probe is inserted into the temperature measuring hole, and the thermocouple probe and the electric heating wire are both connected to the temperature control module;
the optical components comprise a plano-convex lens, a dichroic mirror, a narrow-band filter and a long-wave pass filter, the plano-convex lens and the narrow-band filter are sequentially arranged between the light source module and the dichroic mirror along the light transmission direction, the plano-convex lens is arranged between the dichroic mirror and the fixed pipe sleeve, and the long-wave pass filter and the plano-convex lens are sequentially arranged between the dichroic mirror and the signal detection processing module along the light transmission direction.
Preferably, the side wall of the cavity is provided with a fine adjustment clamping seat, the optical element is arranged on the fine adjustment clamping seat in the cavity, and the fixing pipe sleeve is slidably arranged in the cavity. This configuration allows for optimal collection of the sample fluorescence signal.
Preferably, the fixed pipe sleeve is slidably arranged in the cavity, that is, the lower part of the fixed pipe sleeve is slidably arranged in a groove formed in the base, the fixed pipe sleeve is provided with an adjusting bolt at one side far away from the plano-convex lens, a buffer spring is arranged at one side close to the plano-convex lens, the adjusting bolt is abutted against the fixed pipe sleeve through a through hole formed in the cavity base, the buffer spring is arranged in the groove, one end of the buffer spring is abutted against the wall of the groove, and the other end of the buffer spring is abutted against the fixed pipe sleeve.
Preferably, the output ends of the temperature control module and the signal detection processing module are connected to a control computer.
Preferably, the light source module is provided with a light source adjusting module controlled by a computer.
Compared with the prior art, the utility model has the following advantages:
a. the optical fiber output light source is used as an incident light source, and the bending characteristic of the optical fiber is utilized, so that the module integration is facilitated, and the miniaturization of the whole instrument is facilitated; the optical fiber light source has strong anti-interference performance, the exit angle of the optical fiber is small, and the noise introduced by the surrounding electric signals can be reduced; it is beneficial to the flexible replacement of different (wavelength) light sources according to the difference of the object to be detected in practical detection.
b. The sample amplification and fluorescence signal collection module has a small and compact structure, a sample fixing pipe sleeve with a temperature control module is attached, and an optical device required for collecting fluorescence excited by exciting light is fixed in a cavity of a base, so that optimization and stability of a light path system are facilitated, and stability of an output signal to be detected is guaranteed; the fixed pipe sleeve seals the sample, so that external light interference is avoided, and the problems of sample leakage, sample cross contamination and the like are avoided; the design of the temperature control module can realize the functions of rapid temperature rise and constant temperature; and signal feedback type temperature control is adopted, so that the temperature stability can be improved, and the functions of high-efficiency temperature control and constant temperature are realized.
c. The signal detection processing module is accessed to the photoelectric converter in an optical fiber coupling mode, which is beneficial to modularization of a receiving system and integration of modules and miniaturization of instruments.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
FIG. 2 is an exploded view of the sample amplification and fluorescence signal collection module according to the present invention.
FIG. 3 is a schematic diagram of the optical path transmission of the sample amplification and fluorescence signal collection module according to the present invention.
Fig. 4 is a schematic structural view of the fixing tube socket of the present invention.
Detailed Description
The utility model is described in detail below with reference to the figures and examples.
As shown in fig. 1, 2, 3 and 4, the present invention provides an optical fiber coupling type portable nucleic acid amplification detector, which comprises a light source module a, a sample amplification and fluorescence signal collection module B, a temperature control module D and a signal detection processing module C, and is packaged in a chassis. The light source module A and the sample amplification and fluorescence signal collection module B are coupled and connected through an optical fiber 3, and the sample amplification and fluorescence signal collection module B and the signal detection processing module C are coupled and connected through an optical fiber 3; each module is fixed on the base of the instrument shell through bolts.
The utility model relates to a sample amplification and fluorescence signal collection module B which is in a T-shaped layout and comprises a base 1 and a cavity seat 2, wherein the cavity seat 2 is detachably connected on the base 1 through bolts, the T-shaped cavity seat 2 is provided with a T-shaped cavity, an optical component and a fixed pipe sleeve 7 are arranged in the T-shaped cavity, a fine adjustment clamping seat is arranged on the side wall in the T-shaped cavity, the optical component is arranged on the fine adjustment clamping seat of the T-shaped cavity, the lower end of the fixed pipe sleeve 7 extends onto the base 1 and can slide in a groove 1.1 arranged on the base 1, a light through hole 7.1 and a temperature measuring hole 7.2 are arranged on the upper part of the side wall of the fixed pipe sleeve 7, an electric heating wire 10 is wound on the lower part of the side wall of the fixed pipe sleeve 7, an insulating layer 9 is arranged between the electric heating wire 10 and the side wall of the fixed pipe sleeve 7, the insulating layer 9 is a polyamide film insulating layer, a thermocouple probe is inserted in the temperature measuring hole 7.2, and the thermocouple probe and the electric heating wire 10 are both connected to a temperature control module D, that is, the heating wire 10 is wound around the lower portion of the outer sidewall of the fixing socket 7.
The optical component comprises a plano-convex lens 4, a dichroic mirror 5, a narrow-band filter and a long-wave pass filter 6, wherein the plano-convex lens 4 and the narrow-band filter are sequentially arranged between a light source module A and the dichroic mirror 5 along the light transmission direction, the plano-convex lens 4 is arranged between the dichroic mirror 5 and a fixed pipe sleeve 7, and the long-wave pass filter 6 and the plano-convex lens 4 are sequentially arranged between the dichroic mirror 5 and a signal detection processing module C along the light transmission direction 3. Namely, a light path composed of a plano-convex lens 4 and a narrow-band filter for processing and guiding incident light is positioned in the vertical cavity of the T-shaped cavity chamber, and a light source reflection and fluorescence collection light path composed of a dichroic mirror 5, two plane convex mirrors 4 and a long-pass filter for guiding and processing light source and fluorescence from a sample is positioned in the horizontal cavity of the T-shaped cavity chamber.
When the micro-adjustment clamping seat is implemented specifically, the cavity seat is provided with a fixing groove with the size slightly larger than that of an optical component, the optical component can be subjected to micro-adjustment in the light path direction, the micro-adjustment range is 1mm, the dichroic mirror 5 is subjected to micro-adjustment to rotate, and other components move back and forth in the light path direction. The structure can enable the position of the optical component to be finely adjusted, and coupling transmission of optical signals is optimized.
The fixed pipe sleeve 7 can slide in the groove 1.1 formed in the base 1, namely, the fixed pipe sleeve 7 is provided with an adjusting bolt at one side far away from the plano-convex lens 4, a buffer spring is arranged at one side close to the plano-convex lens 4, the adjusting bolt is propped against the fixed pipe sleeve 7 through a through hole 2.1 arranged on the cavity base 2, the buffer spring is arranged in the groove 1.1, one end of the buffer spring is propped against the wall of the groove 1.1, the other end of the buffer spring is propped against the fixed pipe sleeve 7, and the adjusting bolt is propped against the fixed pipe sleeve 7 to slide in the groove 1.1 by rotating the adjusting bolt. The output ends of the temperature control module D and the signal detection processing module C are connected with a control computer. The light source module A is provided with a light source adjusting module, and the intensity of the light source is controlled by a computer.
When the device is used specifically, blue light output by the optical fiber of the light source module is collimated through the plano-convex lens, a narrow-band light (the bandwidth is less than 3 nm) is emitted through the narrow-band optical filter, and then is reflected to the focusing plano-convex lens through the dichroic mirror, and the focusing plano-convex lens focuses the light to a sample placed in the sample tube to excite the fluorescence of the sample; the fluorescence part scattered by the sample is collimated by the focusing plano-convex lens through the light through hole, then penetrates through the dichroic mirror, passes through the long-pass filter, is finally focused by the plano-convex lens and is coupled into the optical fiber, and the optical fiber guides the signal into a spectrometer or a photoelectric detector to realize the reading and analysis of the signal.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.
Claims (5)
1. An optical fiber coupling type portable nucleic acid amplification detector is characterized in that: the detector comprises a light source module, a sample amplification and fluorescence signal collection module, a temperature control module and a signal detection processing module which are packaged in a case, wherein the light source module is connected with the sample amplification and fluorescence signal collection module through an optical fiber;
the sample amplification and fluorescence signal collection module comprises a base and a cavity seat, the cavity seat is connected to the base, the cavity seat is provided with a cavity for placing an optical component and a fixed pipe sleeve, the upper side wall of the fixed pipe sleeve is provided with a light transmitting hole and a temperature measuring hole, the lower side wall of the fixed pipe sleeve is wound with an electric heating wire, an insulating layer is arranged between the electric heating wire and the side wall of the fixed pipe sleeve, a thermocouple probe is inserted into the temperature measuring hole, and the thermocouple probe and the electric heating wire are both connected to the temperature control module;
the optical components comprise a plano-convex lens, a dichroic mirror, a narrow-band filter and a long-wave pass filter, the plano-convex lens and the narrow-band filter are sequentially arranged between the light source module and the dichroic mirror along the light transmission direction, the plano-convex lens is arranged between the dichroic mirror and the fixed pipe sleeve, and the long-wave pass filter and the plano-convex lens are sequentially arranged between the dichroic mirror and the signal detection processing module along the light transmission direction.
2. The fiber-coupled portable nucleic acid amplification detector of claim 1, wherein: the side wall of the cavity is provided with a fine adjustment clamping seat, the optical component is arranged on the fine adjustment clamping seat in the cavity, and the fixing pipe sleeve is slidably arranged in the cavity.
3. The fiber-coupled portable nucleic acid amplification detector of claim 2, wherein: the fixed pipe sleeve is slidably arranged in the cavity, namely, the lower part of the fixed pipe sleeve slides in a groove arranged on the base, the fixed pipe sleeve is provided with an adjusting bolt on one side far away from the plano-convex lens, one side close to the plano-convex lens is provided with a buffer spring, the adjusting bolt is abutted against the fixed pipe sleeve through a through hole arranged on the cavity seat, the buffer spring is arranged in the groove, one end of the buffer spring is abutted against the wall of the groove, and the other end of the buffer spring is abutted against the fixed pipe sleeve.
4. The fiber-coupled portable nucleic acid amplification detector of claim 1, wherein: the output ends of the temperature control module and the signal detection processing module are connected with a control computer.
5. The fiber-coupled portable nucleic acid amplification detector of claim 1, wherein: the light source module is provided with a light source adjusting module controlled by a computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123412921.3U CN216712107U (en) | 2021-12-31 | 2021-12-31 | Optical fiber coupling type portable nucleic acid amplification detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123412921.3U CN216712107U (en) | 2021-12-31 | 2021-12-31 | Optical fiber coupling type portable nucleic acid amplification detector |
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| CN216712107U true CN216712107U (en) | 2022-06-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202123412921.3U Active CN216712107U (en) | 2021-12-31 | 2021-12-31 | Optical fiber coupling type portable nucleic acid amplification detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115231202A (en) * | 2022-09-21 | 2022-10-25 | 儒克生物科技常州有限公司 | Conveying system for fluorescence detection and working method |
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2021
- 2021-12-31 CN CN202123412921.3U patent/CN216712107U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115231202A (en) * | 2022-09-21 | 2022-10-25 | 儒克生物科技常州有限公司 | Conveying system for fluorescence detection and working method |
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