CN216535298U

CN216535298U - Circulating tumor cell capturing device

Info

Publication number: CN216535298U
Application number: CN202123025273.6U
Authority: CN
Inventors: 吴介恒; 李程程; 胡亚欣
Original assignee: Affiliated Hospital of Guizhou Medical University; Guizhou Medical University
Current assignee: Affiliated Hospital of Guizhou Medical University; Guizhou Medical University
Priority date: 2021-12-04
Filing date: 2021-12-04
Publication date: 2022-05-17
Anticipated expiration: 2031-12-04

Abstract

The utility model relates to the technical field of medical instruments, and provides a circulating tumor cell capturing device which comprises a puncture needle, a capturing net, a connecting pipe, an inflation pipe and an inflation bag, wherein the puncture needle is detachably connected to the front end of the connecting pipe, compact liquid outlet holes are uniformly distributed in the puncture needle, the capturing net is detachably arranged by being attached to the inner wall of the puncture needle, the inflation bag is connected to the tail end of the connecting pipe through an inflation valve, an inflation air bag is arranged at the position, close to the puncture needle, of the outer wall of the connecting pipe, the inflation pipe is respectively connected with the inflation bag and the inflation air bag along the two ends of the inner arm of the connecting pipe, and a plurality of liquid inlet holes are formed in the side wall, close to the inflation air bag, of the connecting pipe and facing one side of the inflation bag. The utility model does not need repeated blood drawing for assay, can relieve the pain of a patient, and can prick the capturing device into the vein to collect the sample for a period of time, so that the sample randomness is low, and the detection accuracy can be improved.

Description

Circulating tumor cell capturing device

Technical Field

The utility model relates to the technical field of medical instruments, in particular to a circulating tumor cell capturing device.

Background

Decades ago, the medical field found that cancer metastasis was largely due to the fact that carcinoma cells were disseminated into the blood by carcinoma in situ and then carried to other organs by the blood, thereby forming new tumors. Currently, the capture and detection of Circulating Tumor Cells (CTCs) has been used as a new diagnostic method for fluid to track the spread of cancer or to predict the prognostic effect of a treatment.

To date, almost all references to the capture and detection of CTCs have been based on the taking of several ml samples from blood, and the capture and detection of CTCs in such samples. The above described trapping has some drawbacks. Firstly, frequent blood drawing with a needle can cause pain to patients. Secondly, the detection result is inaccurate. This is because: the basic rule of sampling in analytical chemistry is that the sample is representative, and the distribution of CTCs is irregular, or there is a cluster of cells that are scattered into the blood, so the distribution of CTCs in the blood is not uniform. This would inevitably result in a random sample from the blood being less representative, and thus would make such sampling-based detection methods highly likely to be false-negative. I.e. the established assay method is able to detect 1 cell in 1 ml of blood, this is not an accurate result with respect to the heterogeneous distribution of CTCs in whole body blood.

In view of the above, there is a need to develop a more accurate and effective device for capturing circulating tumor cells in vivo.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a circulating tumor cell capturing device to solve the problem of the prior art.

The embodiment of the utility model is realized by the following technical scheme:

the utility model provides a circulation tumor cells trapping apparatus, includes the pjncture needle, catches net, connecting pipe, gas tube and gas cell, pjncture needle detachable connects in the front end of connecting pipe, and the equipartition has fine and close liquid hole on the pjncture needle, it can dismantle the setting in the inner wall of pjncture needle to catch the net laminating, the gas cell passes through the inflation valve and connects in the tail end of connecting pipe, the outer wall of connecting pipe is close to in the position department of pjncture needle is provided with inflation airbag, gas tube connects gas cell and inflation airbag respectively along the inner arm both ends of connecting pipe, is close to in inflation airbag, and is provided with a plurality of feed liquor holes towards the connecting pipe lateral wall of gas cell one side.

Furthermore, a thread shoulder is arranged on one side of the connecting pipe, which protrudes towards the puncture needle, and the puncture needle is connected with the thread shoulder in a threaded manner.

Furthermore, the puncture needle is in a conical shape, the capture net is arranged along the inner contour of the puncture needle in a covering mode, and an antibody which is specifically combined with the circulating tumor cells is embedded into the capture net.

Furthermore, the connecting pipe is a hose, and a supporting guide wire is arranged in the connecting pipe.

The technical scheme of the embodiment of the utility model at least has the following advantages and beneficial effects:

the utility model does not need to take blood repeatedly for testing, can not only relieve the pain of the patient, but also prick the capturing device into the vein to collect the sample for a period of time, has small sample randomness and can increase the accuracy of detection.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic structural diagram of a device for capturing circulating tumor cells according to example 1 of the present invention;

FIG. 2 is a partial cross-sectional view of a circulating tumor cell capturing device provided in example 1 of the present invention;

fig. 3 is an enlarged view of a in fig. 2.

Icon: 1-connecting pipe, 2-puncture needle, 3-catching net, 4-thread shoulder, 5-inflation bag, 6-inflation bag, 7-inflation pipe, 8-inflation valve and 9-liquid inlet hole.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Referring to fig. 1 to 3, the embodiment provides a capture device for circulating tumor cells, which comprises a puncture needle 2, a capture net 3, a connecting tube 1, an inflation tube 7 and an inflation bag 5, wherein a threaded shoulder 4 is arranged at the front end of the connecting tube 1, and the puncture needle 2 is screwed on the threaded shoulder 4, so that a detachable connection is formed between the puncture needle 2 and the connecting tube 1. The connecting tube 1 is a hose made of various polymers with good biocompatibility, such as silicon rubber, polyurethane, fluorinated ethylene propylene copolymer (FEP), and is internally provided with a supporting guide wire.

The puncture needle 2 is in a conical shape, a plurality of liquid outlet holes 10 are uniformly distributed on the outer wall of the puncture needle 2, the capture net 3 is attached to the inner wall of the puncture needle 2 and is covered and arranged along the inner contour of the puncture needle 2, the capture net 3 can be taken out from one end of the puncture needle 2 close to the thread shoulder 4, and an antibody specifically combined with circulating tumor cells is embedded into the capture net 3, namely, when the capture net 3 is manufactured, a specific antibody is added. The antibody is one or more of anti-epithelial cell adhesion protein (EpCAM), Cytokeratin (CK), Vascular Endothelial Growth Factor (VEGF), epidermal growth factor receptor 1 (EGFR), proto-oncogene human epidermal growth factor receptor 2 (HER 2) antibodies.

Inflatable bag 5 connects in the tail end of connecting pipe 1 through inflation valve 8, the outer wall of connecting pipe 1 is close to in the butt fusion of position department of pjncture needle 2 has inflation bag 6, inflation tube 7 is connected inflatable bag 5 and inflation bag 6 respectively along the inner arm of connecting pipe 1 and its both ends, through opening inflation valve 8, presses inflatable bag 5 alright aerify with 6 inflation bags of inflation, unclamps inflatable bag 5 alright with 6 disappointing with 6 inflation bags of inflation bag. A plurality of liquid inlet holes 9 are arranged on the side wall of the connecting pipe 1 which is close to the expansion air bag 6 and faces to one side of the air inflation bag 5.

As can be seen from the above, the working principle and the using method of the device for capturing circulating tumor cells provided in this embodiment are as follows:

the front end of the connection tube 1 is first inserted into the venous blood vessel and the inflatable balloon 6 is introduced into the venous blood vessel in a deflated state and the access opening 9 is required to enter the venous blood vessel.

Then, under a visible environment, the inflation valve 8 is opened, the inflation bag 5 is used for inflating the inflation bag 6, the inflation of the inflation bag 6 is observed, the inflation bag 6 is attached to the wall of the venous blood vessel, the inflation valve 8 is closed, the inflation is stopped, at the moment, the blood vessel is blocked by the inflation bag 6, the blood can only flow into the connecting pipe 1 through the liquid inlet hole 9 and then flows out along the liquid inlet pipe through the capture net 3 at the front end and the liquid outlet hole 10 on the puncture needle 2, and therefore the blood is combined with the characteristic antibody on the capture net 3 when flowing through the capture net 3, and the tumor cells are captured;

and finally, after a period of time, opening the inflation valve 8 to enable the gas of the expansion air bag 6 to flow back to the inflation air bag 5, withdrawing the puncture needle 2 after the expansion air bag 6 contracts, taking down the puncture needle 2 from the connecting pipe 1 under the sterile environment, and finally taking out the catching net 3 for detection.

Therefore, the utility model does not need repeated blood drawing and testing, can not only relieve the pain of the patient, but also prick the capturing device into the vein to collect the sample for a period of time instead of randomly drawing the blood for testing, so the randomness of the sample is small, and the accuracy of the detection can be increased.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A circulating tumor cell capturing device, comprising: including pjncture needle (2), catch net (3), connecting pipe (1), gas tube (7) and gas cell (5), pjncture needle (2) detachable connects in the front end of connecting pipe (1), and the equipartition has fine and close liquid hole (10) on pjncture needle (2), it can dismantle the setting to catch net (3) and laminate in the inner wall of pjncture needle (2), gas cell (5) are connected in the tail end of connecting pipe (1) through inflation valve (8), the outer wall of connecting pipe (1) is close to in the position department of pjncture needle (2) is provided with inflation gasbag (6), gas tube (7) are connected gas cell (5) and inflation gasbag (6) respectively along the inner arm both ends of connecting pipe (1), are close to inflation gasbag (6) and are provided with a plurality of feed liquor holes (9) towards connecting pipe (1) lateral wall of gas cell (5) one side.

2. The circulating tumor cell capturing apparatus of claim 1, wherein: the connecting pipe (1) is provided with a thread convex shoulder (4) in a protruding mode towards one side of the puncture needle (2), and the puncture needle (2) is connected to the thread convex shoulder (4) in a threaded mode.

3. The circulating tumor cell capturing apparatus of claim 1, wherein: the puncture needle (2) is in a conical shape, the capture net (3) is arranged along the inner contour of the puncture needle (2) in a covering mode, and antibodies which are specifically combined with circulating tumor cells are embedded into the capture net (3).

4. The circulating tumor cell capturing apparatus of claim 1, wherein: the connecting pipe (1) is a hose, and a supporting guide wire is arranged in the connecting pipe.