CN216505610U - Auxiliary film shearing device in Western Blot film transferring process - Google Patents
Auxiliary film shearing device in Western Blot film transferring process Download PDFInfo
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- CN216505610U CN216505610U CN202123266428.5U CN202123266428U CN216505610U CN 216505610 U CN216505610 U CN 216505610U CN 202123266428 U CN202123266428 U CN 202123266428U CN 216505610 U CN216505610 U CN 216505610U
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- sliding
- groove
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- limiting
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- 238000010008 shearing Methods 0.000 title claims abstract description 14
- 239000012528 membrane Substances 0.000 claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 10
- 238000012546 transfer Methods 0.000 claims abstract description 8
- 239000000758 substrate Substances 0.000 claims description 16
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- 230000006978 adaptation Effects 0.000 abstract description 2
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- 229920002981 polyvinylidene fluoride Polymers 0.000 description 5
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- 238000010586 diagram Methods 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
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- A Measuring Device Byusing Mechanical Method (AREA)
Abstract
The utility model discloses an auxiliary film shearing device in a Western Blot film transfer process, which comprises a base plate and a subplate for measuring the length and the width simultaneously, the surface of the base plate is provided with an L-shaped sliding groove, a turning sliding mechanism used for limiting when the auxiliary plate slides along the surface of the base plate is connected in the sliding groove, the device can control the length and width of the auxiliary plate to be adjusted by moving towards the stop button so as to ensure that the measuring precision is high and different films can be cut, when in implementation, the positioning column can be prevented from deflecting by moving to the stop button, so that the auxiliary plate carries out length measurement operation, then the moving stop button is opened, so that the reference column deflects and carries out the width measurement operation, so operation can obtain the shearing position rapidly, and adjust according to the area of membrane because of length and width, can be quick adaptation different areas' membrane.
Description
Technical Field
The embodiment of the utility model relates to the field of membrane conversion, in particular to an auxiliary membrane shearing device in a Western Blot membrane conversion process.
Background
Blotting refers to a method of transferring a sample to a solid support and detecting the sample by a corresponding probe reaction. Among them, Western Blotting (WB) is a common experimental method for studies of molecular biology, biochemistry, and immunogenetics, and is a method in which a protein is transferred to a membrane and then detected using an antibody. In the specific process of making WB, wherein, the protein separated after electrophoresis is transferred from the gel to a solid phase carrier (such as PVDF membrane), taking PVDF membrane as an example, the PVDF membrane with a suitable size is needed to be cut according to the size of the rubber strip when cutting the rubber, when doing this operation, experimenters often rely on directly estimating the size of the membrane according to the size of the rubber strip, but often make the PVDF membrane cut too big or too small easily like this, because PVDF membrane is expensive, can cause waste when too big, also do not benefit to experimental operation, need to cut again when too small, also can cause waste, can prolong operating time simultaneously.
To this problem patent application number is 201822241628.7, the patent name is a Western Blot membrane cutting device with scale, and it has designed adjustable draw-in groove, through the frame position of adjusting adjustable draw-in groove for adapt to various size wait to cut membrane SDS-PAGE gel, can be quick cut to the membrane of equidimension not promptly.
But the cutting position can only be known to the comparatively loaded down with trivial details needs adjusting the frame many times when using of such operation, in case misoperation if the vibrations lead to the membrane position excursion to appear, just must adjust once more for work efficiency greatly reduced to the less membrane of area, the laminating of adjacent frame very easily appears when removing the frame simultaneously and leads to the condition emergence that the cutting position can't reach the accurate position.
SUMMERY OF THE UTILITY MODEL
Therefore, the embodiment of the utility model provides an auxiliary membrane shearing device in a Western Blot membrane transferring process, and aims to solve the problems that in the prior art, the operation is complicated, the time consumption is long, and the accurate measurement and cutting of a membrane with a small area are difficult.
In order to achieve the above object, an embodiment of the present invention provides the following:
an auxiliary film shearing device in a Western Blot film transferring process comprises a base plate and an auxiliary plate, wherein the base plate and the auxiliary plate are used for measuring the length and the width at the same time, the surface of the base plate is provided with an L-shaped sliding groove, and a transferring sliding mechanism used for limiting when the auxiliary plate slides along the surface of the base plate is connected in the sliding groove;
transfer slide mechanism include with sliding tray bottom inner wall connection's slide bar, be equipped with on the slide bar and be connected and be used for holding the reference column of subplate with the subplate, the both ends of slide bar all are connected with one and are used for inlaying the slide bar and inlay quantitative strip with the cunning of controlling the subplate slip volume, the inside wall of sliding tray is seted up and is inlayed quantitative strip to the cunning and carry out spacing mounting groove be equipped with the limit slide groove on the base plate, limit slide inslot is connected with and is moved to only button.
As a preferable scheme of the utility model, the positioning column includes a deflection sleeve connected with the sliding strip, a sliding sheet is connected in the deflection sleeve, a tension spring connected with the inner wall of the bottom of the deflection sleeve is installed on the surface of one side of the sliding sheet, a deformation strip is installed on the surface of the other side of the sliding sheet, and a deflection block connected with the auxiliary plate and driving the deflection sleeve to move is installed at one end of the deformation strip away from the sliding sheet.
As a preferable scheme of the present invention, the movement direction stopping button includes a sliding pushing column connected to an inner wall of the sliding limiting groove, a lateral separation sliding block for preventing the deflection sleeve from deflecting in the sliding groove is installed on a surface of one side of the sliding pushing column away from the substrate, and a wide limiting column having an i-shaped structure and being pushed by the sliding pushing column is arranged at a position on the surface of the substrate corresponding to the sliding limiting groove.
As a preferable scheme of the utility model, an embedding groove for embedding the width limiting column is formed on the surface of one side of the auxiliary plate close to the base plate, a plurality of width measuring points are installed on the inner wall of the embedding groove, and a through groove which is connected with the deflection block and used for accommodating the movement of the deflection sleeve is formed at the end part of the auxiliary plate.
As a preferable scheme of the present invention, the inner wall of the bottom of the sliding groove is provided with a sliding strip limiting groove for limiting the sliding strip.
The embodiment of the utility model has the following advantages:
the device accessible moves to the knob, and the control subplate carries out the regulation operation of length and width so that measurement accuracy height can be cut different membranes, and when it was implemented, the accessible moved to the knob and prevents the reference column deflection to make the subplate carry out length measurement operation, later opened again and moved to the knob, so that the reference column deflection carries out width measurement operation, so the operation can obtain the shearing position rapidly, and adjust according to the area of membrane because of length and width, the different area's of adaptation that can be quick membrane.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
FIG. 1 is a schematic overall structure diagram of an embodiment of the present invention;
FIG. 2 is a schematic view of a positioning post according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a sliding groove according to an embodiment of the present invention.
In the figure:
1-a substrate; 2-a secondary plate; 3-a sliding groove; 4-turning the sliding mechanism; 5-limiting the groove by a sliding strip;
21-embedding the groove; 22-measuring width points; 23-a through groove;
41-a sliding bar; 42-a positioning column; 43-slip embedding quantitative strips; 44-mounting grooves; 45-limit sliding chutes; 46-move towards stop button;
421-deflection sleeve; 422-a sliding sheet; 423-tension spring; 424-deformation strips; 425-deflection block;
461-push sliding column; 462-lateral spacer slider; 463-Width Limit column.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the utility model will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the utility model and that it is not intended to limit the utility model to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in FIG. 1, the utility model provides an auxiliary film shearing device in the process of Western Blot film transfer, which comprises a substrate 1 and a subplate 2 for measuring length and width simultaneously, wherein the surface of the substrate 1 is provided with a sliding groove 3 in an L-shaped structure, and the sliding groove 3 is internally connected with a transfer sliding mechanism 4 for limiting when the subplate 2 slides along the surface of the substrate 1;
transfer slide mechanism 4 and include the sliding strip 41 with 3 bottom inner wall connections of sliding tray, be equipped with on the sliding strip 41 and be connected with subplate 2 and be used for pulling the reference column 42 of subplate 2, the both ends of sliding strip 41 all are connected with one and are used for inlaying sliding strip 41 with the smooth quantitative strip 43 of inlaying of controlling the subplate 2 slippage, sliding tray 3's inside wall has been seted up and has been carried out spacing mounting groove 44 to smooth quantitative strip 43 of inlaying, be equipped with limited sliding groove 45 on base plate 1, it is connected to move to only button 46 to limit sliding groove 45 in-connection.
The device can realize the sliding regulation operation of the auxiliary plate 2 by regulating the sliding mechanism 4, so that a measuring scale (the measuring scale is used for regulating the length and the width) consisting of the base plate 1 and the auxiliary plate 2 can synchronously carry out the length and the width operation for improving the working efficiency, when the device is implemented, the device can directly drive the moving stop button 46 to stop the sliding groove 3, at the moment, the positioning column 42 can not deflect, namely, the width of the measuring scale can not be increased, but only the length can be increased, then, when the sliding strip 41 slides to the end part along the sliding groove 3, the auxiliary plate 2 can be directly pushed to press the end part of the base plate 1, the state is shown in figure 2, the length regulation of the measuring scale is finished, if the regulation is not needed, the auxiliary plate 2 can be pressed on the base plate 1 to form an overlapped state, and in the quantifying process, the sliding embedded strip 43 can play a role in positioning, so that the amount of slide bar 41 per slide adjustment is fixed, and the mounting groove 44 may be used to engage the slide bar 41 so that the slide bar 41 does not slip out of the slide groove 3.
If the width of the measuring scale needs to be adjusted, the auxiliary plate 2 is only pushed to move to the stop button 46 to release the sliding groove 3, then the auxiliary plate 2 is only pushed to drive the positioning column 42 to rotate, then the positioning column 42 deflects in the sliding groove 3, and after the positioning column 42 is laid down and enters the sliding groove 3, the auxiliary plate 2 is firmly attached to the base plate 1, so that the purpose of adjusting the width of the measuring scale is achieved.
In this embodiment, the number of the sub-plates 2 may be 1, 2 or 3, and the connection is also performed according to the above embodiment and the turning sliding mechanism 4 shown in fig. 1 of the attached drawings, and only the previous sub-plate 2 is used as the base plate 1 in the specific arrangement, so that the current sub-plate 2 can be smoothly fixed, and the sliding-embedding quantitative bar 43 may be composed of a straight bar in the shape of "L" or "u" and a plurality of protruding fixed points installed on the inner wall of the straight bar.
As shown in fig. 1 and 2, the positioning column 42 includes a deflection sleeve 421 connected to the sliding bar 41, a sliding piece 422 is connected to the deflection sleeve 421, a tension spring 423 connected to an inner wall of the bottom of the deflection sleeve 421 is installed on a surface of one side of the sliding piece 422, a deformation strip 424 is installed on a surface of the other side of the sliding piece 422, and a deflection block 425 connected to the sub-plate 2 and driving the deflection sleeve 421 to move is installed on one end of the deformation strip 424 away from the sliding piece 422.
When the sub-plate 2 moves, the length adjustment is taken as an example for explanation here, the deflecting block 425 is directly driven, then the movable deflecting block 425 pulls the deforming bar 424 and the sliding piece 422 to move together, then the movable sliding piece 422 sequentially pulls the tension spring 423, the deflecting sleeve 421 and the sliding bar 41, and the sliding bar 41 slides along the sliding groove 3, then, when the sliding bar 41 is clamped at the end of the sliding groove 3, if the sub-plate 2 continues to move, the sliding piece 422 and the deforming bar 424 are pulled, and the tension spring 423 is stretched, then, as the sub-plate 2 moves, the deflecting sleeve 421 gradually tilts and simultaneously stretches the tension spring 423 until the sub-plate 2 assumes the state shown in fig. 2.
In this embodiment, the tension spring 423 is always in a tension state, and only in this state, the purpose of pulling the base plate 1 through the sub plate 2 can be satisfied, and the deformation strip 424 may be a rubber strip or a spring strip.
As shown in fig. 1 and 3, the movement direction stopping button 46 includes a sliding pushing column 461 connected to an inner wall of the sliding limiting groove 45, a lateral separating slider 462 for blocking the deflection of the deflection sleeve 421 in the sliding groove 3 is installed on a surface of the sliding pushing column 461 at a side away from the substrate 1, and a width limiting column 463 which is in an "i" shape and is pushed by the sliding pushing column 461 is penetratingly installed at a position on the surface of the substrate 1 corresponding to the sliding limiting groove 45.
In daily life, if the deflection sleeve 421 needs to be blocked so as not to incline, the side partition slider 462 is pushed to slide to the position shown in fig. 1 and 3, if the width of the measuring scale needs to be adjusted, the side partition slider 462 is pushed in the opposite direction so as to release the sliding groove 3, at this time, the side partition slider 462 can drive the push sliding column 461 to slide along the limiting sliding groove 45, then, when the push sliding column 461 moves to be right below the wide limit column 463, the side partition slider 462 can be pushed to ascend, at this time, the ascending side partition slider 462 can push the wide limit column 463 to ascend until the wide limit column 463 is not lifted.
In this embodiment, a movable hole is formed between the slide-limiting slot 45 and the lateral partition slider 462, so that the lateral partition slider 462 can slide normally.
As shown in fig. 1 and 2, an embedding groove 21 for embedding the width limiting pillar 463 is formed in a surface of one side of the sub-plate 2 close to the substrate 1, a plurality of width measuring points 22 are installed on an inner wall of the embedding groove 21, and a through groove 23 connected with the deflection block 425 and used for accommodating the movement of the deflection sleeve 421 is formed in an end portion of the sub-plate 2.
When the wide limit post 463 risees, can directly imbed and inlay recess 21 (the prerequisite is that subplate 2 overlaps on base plate 1 surface), later if promote subplate 2 lateral sliding (because of wide limit post 463 can not slide along base plate 1 horizontal, can only move about, and at this in-process, gliding subplate 2 can slide along wide limit post 463, survey wide point 22 simultaneously and can play the purpose spacing and accurate removal interval, make the volume that subplate 2 removed at every turn fixed, and when subplate 2 lateral hugs closely base plate 1, it can hold the deflection cover 421 to lead to groove 23, make the laminating operation not influenced.
The inner wall of the bottom of the sliding groove 3 is provided with a sliding strip limiting groove 5 for limiting the sliding strip 41, and the sliding strip limiting groove 5 is used for limiting the position of the sliding strip 41 so that the sliding strip cannot be directly separated from the substrate 1.
Although the utility model has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the utility model. Accordingly, such modifications and improvements are intended to be within the scope of the utility model as claimed.
Claims (5)
1. An auxiliary film shearing device in a Western Blot film transfer process is characterized by comprising a substrate (1) and a subplate (2) which are used for measuring length and width simultaneously, wherein the surface of the substrate (1) is provided with a sliding groove (3) in an L-shaped structure, and a transfer sliding mechanism (4) for limiting when the subplate (2) slides along the surface of the substrate (1) is connected in the sliding groove (3);
transfer slide mechanism (4) and include sliding strip (41) with sliding tray (3) bottom inner wall connection, be equipped with on sliding strip (41) and be connected and be used for pulling reference column (42) of subplate (2) with subplate (2), the both ends of sliding strip (41) all are connected with one and are used for inlaying sliding strip (41) with the cunning of controlling subplate (2) sliding volume inlay ration strip (43), the inside wall of sliding tray (3) is seted up and is carried out spacing mounting groove (44) to cunning inlay ration strip (43) be equipped with limit sliding groove (45) on base plate (1), limit sliding groove (45) in-connection has to move to only button (46).
2. The auxiliary membrane shearing device in the Western Blot membrane rotating process according to claim 1, wherein the positioning column (42) comprises a deflection sleeve (421) connected with a sliding strip (41), a sliding sheet (422) is connected in the deflection sleeve (421), a tension spring (423) connected with the inner wall of the bottom of the deflection sleeve (421) is installed on one side surface of the sliding sheet (422), a deformation strip (424) is installed on the other side surface of the sliding sheet (422), and a deflection block (425) which is connected with the auxiliary plate (2) and drives the deflection sleeve (421) to move is installed at one end, far away from the sliding sheet (422), of the deformation strip (424).
3. The auxiliary membrane shearing device in the Western Blot membrane transferring process according to claim 1, wherein the moving direction stop button (46) comprises a push-slide column (461) connected with the inner wall of the limiting slide groove (45), a lateral separation slide block (462) for blocking the deflection of the deflection sleeve (421) in the slide groove (3) is installed on one side surface of the push-slide column (461) far away from the substrate (1), and a wide limiting column (463) which is in an I-shaped structure and is pushed by the push-slide column (461) is arranged on the surface of the substrate (1) at a position corresponding to the limiting slide groove (45) in a penetrating manner.
4. The auxiliary membrane shearing device in the Western Blot membrane transferring process is characterized in that an embedding groove (21) for embedding a width limiting column (463) is formed in the surface, close to a substrate (1), of the auxiliary plate (2), a plurality of width measuring points (22) are installed on the inner wall of the embedding groove (21), and a through groove (23) which is connected with a deflection block (425) and used for accommodating the movement of a deflection sleeve (421) is formed in the end portion of the auxiliary plate (2).
5. The auxiliary membrane shearing device in the Western Blot membrane transferring process according to claim 1, wherein a sliding strip limiting groove (5) for limiting a sliding strip (41) is formed in the inner wall of the bottom of the sliding groove (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123266428.5U CN216505610U (en) | 2021-12-23 | 2021-12-23 | Auxiliary film shearing device in Western Blot film transferring process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123266428.5U CN216505610U (en) | 2021-12-23 | 2021-12-23 | Auxiliary film shearing device in Western Blot film transferring process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216505610U true CN216505610U (en) | 2022-05-13 |
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ID=81502587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123266428.5U Expired - Fee Related CN216505610U (en) | 2021-12-23 | 2021-12-23 | Auxiliary film shearing device in Western Blot film transferring process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216505610U (en) |
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2021
- 2021-12-23 CN CN202123266428.5U patent/CN216505610U/en not_active Expired - Fee Related
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Granted publication date: 20220513 |