CN216492783U - Portable bee collection test device for detecting potential toxicity of pesticide on bee development - Google Patents

Portable bee collection test device for detecting potential toxicity of pesticide on bee development Download PDF

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Publication number
CN216492783U
CN216492783U CN202123194146.9U CN202123194146U CN216492783U CN 216492783 U CN216492783 U CN 216492783U CN 202123194146 U CN202123194146 U CN 202123194146U CN 216492783 U CN216492783 U CN 216492783U
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bee
queen bee
box
vertical
test device
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CN202123194146.9U
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赵洪木
尤方东
杨爽
张洋逸
李雨时
苗春辉
宋文菲
杨娟
王艳辉
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RESEARCH INSTITUTE OF SILKWORMS MULBERRIES AND BEES YUNNAN ACADEMY OF AGRICULTURAL SCIENCES
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RESEARCH INSTITUTE OF SILKWORMS MULBERRIES AND BEES YUNNAN ACADEMY OF AGRICULTURAL SCIENCES
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Abstract

The utility model discloses a portable bee collection test device for detecting pesticide toxicity potentially to bee development, which comprises a vertical queen bee separation box with an upper end opening, wherein the side walls of the front end and the rear end of the vertical queen bee separation box are made of vertical queen bee separation plates, one edge of the open end of the vertical queen bee separation box is hinged with an openable box cover, the vertical queen bee separation box is connected with a bee embryo collection plate sliding along the height direction of the vertical queen bee separation box in a sliding manner, the upper ends of the left side and the right side of the vertical queen bee separation box are respectively provided with a plurality of permanent magnets, and the left side and the right side of the inner wall of the box cover are respectively provided with a plurality of iron sheets which correspond to the permanent magnets one by one and are used for matching and fixing the box cover. When the method is used for experiments, the problems of long time consumption for artificial larva transfer, easy damage to bee embryos or larvae and low survival rate in the experiment process can be effectively solved, the basic requirements of the experiment on the quantity and quality of biological samples are met, and the accuracy of the detection test method is improved.

Description

Portable bee collection test device for detecting potential toxicity of pesticide on bee development
Technical Field
The utility model relates to the technical field of pesticide toxicity detection, in particular to a portable bee collection test device for detecting potential toxicity of pesticide on bee development.
Background
In agricultural production, when chemical agents such as pesticides are used for killing pests and protecting crops, the influence on pollinating insects such as bees is inevitable, generally speaking, collected bees are easy to be poisoned by contacting risk factors such as pesticides, however, when the collected bees bring pollen and honeydew containing residual agents back to the bees to be stored as bee grains or to be directly eaten by other bees, the residual agents can cause risks to bees of different grades in the whole bees in the bees, bee embryos are early physiological forms of the bees which can contact pesticides, and the influence on the subsequent life history of the bees can be different degrees after the bees are contacted with the agents in the egg stage and the larva stage.
In the test of influence of exposure treatment of indoor evaluation pesticides and other compounds on the subsequent growth and development of bees in the embryonic stage and the larval stage of the bees, the bee embryos are difficult to collect, easy to damage, low in survival rate, large in workload of artificial larva transfer and long in time consumption, and the bee embryos can be damaged if improper operation is carried out when the larva transfer needle is used, so that the basic requirements of conventional tests on the quantity and quality of biological samples cannot be met, and therefore, the portable bee collection test device for detecting the potential toxicity of the pesticides on bee development is designed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a portable bee collecting test device for detecting pesticide development potential toxicity on bees, which can better realize quick and damage-free larva collecting operation in the detection process of pesticide development potential toxicity on bees during experiments, thereby effectively overcoming the problems of long time consumption of artificial larva transfer, easy damage of bee embryos or larvae and low survival rate in the test process, meeting the basic requirements of the tests on the quantity and quality of biological samples and improving the accuracy of the detection test method.
The technical purpose of the utility model is realized by the following technical scheme:
a portable bee collecting test device for detecting potential toxicity of pesticide on bee development comprises a vertical queen bee isolation box with an opening at the upper end, the side walls of the front end and the rear end of the vertical queen bee separating box are made of vertical queen bee separating plates, one side of the open end of the vertical queen bee separating box is hinged with an openable box cover, the vertical queen bee separating box is connected with a bee embryo collecting plate which slides along the height direction in a sliding way, the bee embryo collecting plate comprises a plurality of fixing grooves which are inwards sunken at the upper ends and distributed in an array way, a plurality of collecting boxes which correspond to the fixing grooves one by one and are detachably connected, the two ends of each collecting box are provided with limiting pieces, a bottom sheet which slides along the length direction of each collecting box is connected in a sliding way in each collecting box, the upper end that vertically separates the king case left and right sides all is provided with a plurality of permanent magnets, the left and right sides of case lid inner wall all be provided with a plurality ofly with the permanent magnet one-to-one just is used for the cooperation to be fixed the iron sheet of case lid.
By adopting the technical scheme, when the device is used, the box cover is opened, the bee embryo collecting plate is placed in the vertical queen separating box, the box cover is covered and is transferred into the beehive of a healthy bee colony, the permanent magnet can adsorb the corresponding iron sheet for fixing the box cover, a queen bee is controlled at the inner side of the vertical queen separating box to lay eggs on the bee embryo collecting plate, the queen bee is moved out of the vertical queen separating box after laying eggs for 12 hours, and the honeycomb is continuously kept to be placed in the beehive for 12 hours, so that the bee embryo is fed and cleaned by worker bees;
when the experiment detection is required, the bee embryo collecting plate is taken out, the collecting box is taken down from the bee embryo plate, the bottom plate in the corresponding collecting box is pushed, the bee embryo is pushed out from the collecting box, the rapid and damage-free larva collecting operation in the detection process of pesticide on the bee development potential toxicity can be better realized, the problems of long time consumption of artificial larva transfer, easy damage of the bee embryo or larva and low survival rate in the test process are effectively solved, the basic requirements of the experiment on the quantity and the quality of biological samples are met, and the accuracy of the detection test method is improved.
The utility model is further provided with: and a plurality of reset springs are connected between the upper end of the bottom sheet and the inner wall of the upper limiting sheet of the collecting box.
Through adopting above-mentioned technical scheme, get the bee after finishing, not only can promote the film after the activity to initial position and can also let between film and the spacing piece that corresponds collection box lower extreme sealed.
The utility model is further provided with: the vertical queen bee separating box and the bee embryo collecting plate are both made of organic polymer materials.
Compared with the prior art, the utility model has the following beneficial effects:
firstly, the method can take out the embryo or the larva without taking a worm needle, and can better realize the quick and damage-free larva collection operation in the detection process of potential toxicity of pesticide to bee development;
secondly, the vertical queen bee separation plate utilizes the difference between the thickness of queen bee and the breast thickness of worker bees, and the width of the separation grid is designed to be between the queen bee and the worker bees, so that the worker bees can freely pass through the separation grid, and the movement of the queen bee is limited;
thirdly, the return spring of the utility model not only can push the movable negative to the initial position, but also can seal the negative and the limiting sheet corresponding to the lower end of the collecting box.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a view mainly used for showing the position connection relationship of each component in the vertical queen bee separating box;
FIG. 3 is a view mainly showing a bee embryo collection plate;
fig. 4 is primarily for showing the collection box.
In the figure: 1. a vertical king box; 11. a box cover; 12. a vertical queen excluder; 13. a permanent magnet; 14. iron sheets; 2. a bee embryo collection plate; 21. fixing grooves; 22. a collection box; 23. a limiting sheet; 24. a negative film; 25. a return spring.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
The embodiment, referring to fig. 1-4, a portable bee collecting test device for detecting pesticide toxicity to bee development potential comprises a vertical queen bee separating box 1 with an open upper end, wherein the side walls of the front end and the rear end of the vertical queen bee separating box 1 are both made of vertical queen bee separating plates 12, one edge of the open end of the vertical queen bee separating box 1 is hinged with an openable box cover 11, the vertical queen bee separating box 1 is connected with a plurality of bee embryo collecting plates 2 sliding along the height direction in a sliding manner, each embryo collecting plate 2 comprises a plurality of fixing grooves 21 which are inwards recessed at the upper end and distributed in an array manner, a plurality of collecting boxes 22 which are corresponding to the fixing grooves 21 in a one-to-one manner and are detachably connected with openings at two ends, two ends of each collecting box 22 are provided with a limiting piece 23, a bottom piece 24 sliding along the length direction is connected in the collecting boxes 22 in a sliding manner, the upper ends of the left side and the right side of the vertical queen bee separating box 1 are provided with two permanent magnets 13, the left side and the right side of the inner wall of the box cover 11 are respectively provided with two iron sheets 14 which are in one-to-one correspondence with the permanent magnets 13 and are used for matching and fixing the box cover 11, five reset springs 25 are connected between the upper end of the bottom sheet 24 and the inner wall of the limiting sheet 23 at the upper end of the collecting box 22, and the vertical queen separating box 1 and the bee embryo collecting plate 2 are made of organic polymer materials.
The use method comprises the following steps: during the use, open case lid 11, place bee embryo collecting plate 2 in vertical king case 1 that separates, cover case lid 11, and transfer to in the beehive of healthy bee colony, permanent magnet 13 can adsorb the iron sheet 14 who corresponds and live, be used for fixed case lid 11, control the queen bee at the vertical inboard that separates queen bee case 1, make it lay eggs on bee embryo collecting plate 2, shift out vertical king case 1 that separates with it after the queen bee is laid eggs 12h, continue to keep the honeycomb to place 12h in the beehive, so that the bee embryo obtains feeding and the clearance of worker's bee.
When the experiment detection is required, the bee embryo collecting plate 2 is taken out, the collecting box 22 is taken down from the bee embryo plate, the bottom plate 24 in the corresponding collecting box 22 is pushed, the bee embryos are pushed out from the collecting box 22, the bottom plate 24 can be automatically pushed back to the initial position by the reset spring 25 in the corresponding collecting box 22, the rapid and damage-free larva collecting operation in the detection process of potential toxicity of pesticide to bee development can be well realized, the problems of long time consumption of artificial larva moving, easy damage of the bee embryos or the larvae and low survival rate in the test process are effectively solved, the basic requirements of the test on the quantity and the quality of biological samples are met, and the accuracy of the detection test method is improved.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims (3)

1. The utility model provides a test device is collected to portable honeybee of detection pesticide to latent toxicity of honeybee development, includes that the upper end open-ended is vertical separates queen bee case (1), its characterized in that: the side wall that vertically separates both ends around queen bee case (1) is made by vertical queen bee board (12), vertically separate one side of queen bee case (1) open end and articulate there is case lid (11) that can open, vertically separate in queen bee case (1) sliding connection have along its gliding bee embryo collecting plate of direction of height (2), bee embryo collecting plate (2) include a plurality of in its upper end inwards sunken and be array distribution's fixed slot (21), a plurality of with fixed slot (21) one-to-one and can dismantle both ends open-ended collection box (22) of connection, the both ends of each collection box (22) all are provided with spacing piece (23), all sliding connection has along its length direction gliding film (24) in each collection box (22), vertically separate the upper end of queen bee case (1) left and right sides all be provided with a plurality of permanent magnet (13), the left and right sides of case lid (11) inner wall all be provided with permanent magnet (13) one-to-one and be used for cooperating fixed An iron sheet (14) of the box cover (11).
2. The portable bee collection test device for detecting the potential toxicity of pesticides on bee development according to claim 1, which is characterized in that: a plurality of reset springs (25) are connected between the upper end of the bottom sheet (24) and the inner wall of the upper end limiting sheet (23) of the collecting box (22).
3. The portable bee collection test device for detecting the potential toxicity of pesticides on bee development according to claim 1, which is characterized in that: the vertical queen bee separating box (1) and the bee embryo collecting plate (2) are both made of organic polymer materials.
CN202123194146.9U 2021-12-16 2021-12-16 Portable bee collection test device for detecting potential toxicity of pesticide on bee development Active CN216492783U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202123194146.9U CN216492783U (en) 2021-12-16 2021-12-16 Portable bee collection test device for detecting potential toxicity of pesticide on bee development

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Application Number Priority Date Filing Date Title
CN202123194146.9U CN216492783U (en) 2021-12-16 2021-12-16 Portable bee collection test device for detecting potential toxicity of pesticide on bee development

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115486420A (en) * 2022-10-25 2022-12-20 重庆师范大学 Bee nondestructive egg-stage queen bee rearing method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115486420A (en) * 2022-10-25 2022-12-20 重庆师范大学 Bee nondestructive egg-stage queen bee rearing method
CN115486420B (en) * 2022-10-25 2023-06-13 重庆师范大学 Bee nondestructive egg queen breeding method

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