CN216387550U - Super-resolution microscope system - Google Patents
Super-resolution microscope system Download PDFInfo
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- CN216387550U CN216387550U CN202122348797.2U CN202122348797U CN216387550U CN 216387550 U CN216387550 U CN 216387550U CN 202122348797 U CN202122348797 U CN 202122348797U CN 216387550 U CN216387550 U CN 216387550U
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Abstract
The utility model relates to the technical field of optical microscopic imaging, and discloses a super-resolution microscope system, which comprises: the light source is sequentially provided with a first single lens, a second single lens, a double cemented lens, a spatial light processing module, a dichroic mirror and a microscope objective lens from a sample; the first single lens and the second single lens are both single lenses of a glass spherical mirror; the spatial light processing module comprises a spatial light modulator and a first plane mirror; the microscope objective, the dichroic mirror, the second plane reflector and the super-resolution cylindrical mirror are sequentially arranged from the sample to the super-resolution image surface. The whole super-resolution optical path structure is simple in design, the collimation and beam expansion effects on laser are realized through the combination of the two single lenses and the double cemented lens, the chromatic aberration of visible light is eliminated, the first single lens and the second single lens are both single lenses of glass spherical lenses, and the cost is low.
Description
Technical Field
The utility model relates to the technical field of optical microscopic imaging, in particular to a super-resolution microscope system.
Background
With the continuous development of science and technology, people have a continuous and deep understanding on optical microscopic imaging, and meanwhile, higher requirements are also put forward. The aim of continuous pursuit is to look more thin and clear. In order to solve this problem, a series of corresponding technologies are also brought forward. Researchers in various countries proposed structured light super-resolution microscope (SIM) in 2006.
In the ultrahigh resolution equipment in the prior art, functions such as super-resolution collimation and the like are realized by adopting an aspheric lens, the equipment cost is high, and the light path design is complex.
The above is only for the purpose of assisting understanding of the technical aspects of the present invention, and does not represent an admission that the above is prior art.
SUMMERY OF THE UTILITY MODEL
The utility model mainly aims to provide a super-resolution microscope system, and aims to solve the technical problems that in the prior art, an aspheric lens is adopted in super-resolution equipment to realize super-resolution collimation and other functions, the equipment cost is high, and the optical path design is complex.
To achieve the above object, the present invention provides a super-resolution microscope system comprising:
the light source is sequentially provided with a first single lens, a second single lens, a double cemented lens, a spatial light processing module, a dichroic mirror and a microscope objective lens from a sample; the first single lens and the second single lens are both single lenses of a glass spherical mirror; the spatial light processing module comprises a spatial light modulator and a first plane mirror;
the light source emits laser, the first single lens and the second single lens expand the laser, the double cemented lens collimates the expanded light beam, the spatial light modulator collimates the collimated expanded light beam after direct expansion, the first plane mirror reflects the modulated light to the dichroic mirror, and the dichroic mirror reflects the modulated light to the microscope objective;
the microscope objective converges the modulated light on an objective focal plane to excite a sample dyed by fluorescence and to excite fluorescence of a specific waveband;
the microscope objective, the dichroic mirror, the second plane reflector and the super-resolution cylindrical mirror are sequentially arranged from the sample to the super-resolution image surface;
the dichroic mirror passes the fluorescence of the specific wavelength band, and the second plane mirror reflects the fluorescence of the specific wavelength band passing through the dichroic mirror to the super-resolution cylindrical mirror;
and the super-resolution cylindrical lens converges the fluorescence of the specific wave band and images at the super-resolution image surface.
Preferably, the double cemented lens comprises three faces, two of which are concave and one of which is convex; and the double cemented lens is a double cemented lens of a glass spherical mirror.
Preferably, the light source is a laser, and the wavelength band of the laser comprises 532nm or 638 nm.
Preferably, the first plane mirror is movable.
Preferably, the first single lens, the second single lens and the double cemented lens form a super-resolution collimating module, the central axes of the light source and the super-resolution collimating module are both parallel to an X-axis of a spatial coordinate system, and the first plane mirror is placed at a preset angle with the X-axis;
the dichroic mirror is placed in parallel with the first plane reflecting mirror, the second plane reflecting mirror is placed at the preset angle with respect to the Z axis of the space coordinate system, and the central axis of the super-resolution cylindrical mirror is parallel to the Z axis.
Preferably, the preset angle is 45 ° or 135 °.
In the present invention, the super-resolution microscope system includes: the light source is sequentially provided with a first single lens, a second single lens, a double cemented lens, a spatial light processing module, a dichroic mirror and a microscope objective lens from a sample, wherein the first single lens and the second single lens are single lenses of a glass spherical mirror, the spatial light processing module comprises a spatial light modulator and a first plane mirror, the light source emits laser, the first single lens and the second single lens expand the laser, the double cemented lens collimates the expanded light beam, the spatial light modulator is aligned with the collimated expanded light beam and modulates the collimated expanded light beam, the first plane mirror reflects the modulated light to the dichroic mirror, the dichroic mirror reflects the modulated light to the microscope objective lens, the microscope objective converges the modulated light on the focal plane of the objective lens to excite the sample dyed by fluorescence and excite the fluorescence with a specific waveband, the microscope objective, the dichroic mirror, the second plane reflector and the super-resolution cylindrical lens are sequentially arranged from the sample to the super-resolution image surface, the dichroic mirror passes through the fluorescence of the specific wave band, the second plane reflector reflects the fluorescence of the specific wave band passing through the dichroic mirror to the super-resolution cylindrical lens, and the super-resolution cylindrical lens converges the fluorescence of the specific wave band and images at the super-resolution image surface. The whole super-resolution optical path structure is simple in design, the collimation and beam expansion effects on laser are realized through the combination of the two single lenses and the double cemented lens, the chromatic aberration of visible light is eliminated, the first single lens and the second single lens are both single lenses of glass spherical lenses, and the cost is low.
Drawings
FIG. 1 is a schematic structural diagram of a super-resolution microscope system according to an embodiment of the present invention.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.
Referring to fig. 1, an embodiment of a super-resolution microscope system of the present invention is presented.
In this embodiment, the super-resolution microscope system includes:
the light source 01 is provided with a first single lens 02, a second single lens 03, a double cemented lens 04, a space light processing module 05, a dichroic mirror 06 and a microscope objective 07 in sequence from the sample; the first single lens 02 and the second single lens 03 are both single lenses of glass spherical lenses; the spatial light processing module 05 comprises a spatial light modulator and a first plane mirror;
the light source 01 emits laser light, the first single lens 02 and the second single lens 03 expand the laser light, the double cemented lens 04 collimates the expanded light beam, the spatial light modulator aligns the collimated expanded light beam after direct expansion and modulates the collimated expanded light beam, the first plane mirror reflects the modulated light to the dichroic mirror 06, and the dichroic mirror 06 is used for reflecting the modulated light to the microscope objective 07;
the microscope objective 07 converges the modulated light on an objective focal plane to excite a sample stained by fluorescence and to excite fluorescence of a specific waveband;
the microscope objective 07, the dichroic mirror 06, the second plane mirror 08 and the super-resolution cylindrical mirror 09 are arranged from the sample to the super-resolution image surface in sequence;
the dichroic mirror 06 passes the fluorescence of the specific wavelength band, and the second plane mirror 08 reflects the fluorescence of the specific wavelength band passing through the dichroic mirror 06 to the super resolution tube mirror 09;
the super-resolution cylindrical lens 09 converges the fluorescence of the specific wave band, and images are formed on the super-resolution image surface.
It should be understood that the light source 01 may be a laser, and the laser emitted by the laser is visible light with a wavelength within 400-700 nm. The system also includes a controller that controls the switching of the laser. The system also comprises a computer which is used as an upper computer of the controller.
It should be noted that the first single lens 02, the second single lens 03, and the double cemented lens 04 constitute a super-resolution collimating module, the first single lens 02 and the second single lens 03 expand the laser, and the double cemented lens 04 collimates the expanded beam. The modulated light reaches the spatial light processing module 05 and is reflected to the dichroic mirror 06, the dichroic mirror 06 reflects the modulated light to the microscope objective 07, the modulated light enters the microscope objective 07 and is converged on the objective focal plane, the sample which is subjected to fluorescent staining is excited, and fluorescence of the specific waveband is excited, the value range of the specific waveband is 558-680, and the specific waveband is determined according to different fluorescent dyes.
In a specific implementation, the laser light cannot pass through the dichroic mirror 06, and is reflected into the microscope objective 07 when the laser light reaches the dichroic mirror 06. The fluorescence in the specific waveband can pass through the dichroic mirror 06, and when the fluorescence in the specific waveband reaches the dichroic mirror 06, the fluorescence passes through the dichroic mirror 06, is reflected by the second plane reflecting mirror 08, enters the super-resolution cylindrical mirror 09, and is finally imaged on the super-resolution image plane.
It can be understood that, when the super-resolution microscope system needs to be coupled with the confocal microscope system, the controller controls to remove the first plane mirror, and then the confocal microscope system can utilize the dichroic mirror 06 and the microscope objective 07 in the super-resolution microscope system to reduce the cost of designing the optical path, and when the super-resolution microscope system needs to be used, the controller controls to move the first plane mirror into the optical path from the light source 01 to the microscope objective 07.
Further, the double cemented lens 04 comprises three faces, two of which are concave and one of which is convex; and, the double cemented lens 04 is a double cemented lens 04 of a glass spherical mirror.
In this embodiment, the front 2 single lenses expand the beam of the fiber-coupled laser, and the rear first cemented doublet 04 collimates the light source 01. The whole system flexibly combines a single lens and a cemented lens to match the NA of the output optical fiber of the laser, eliminates the chromatic aberration of visible light (400-700nm), and can be suitable for the light source 01 of the waveband. First single lens 02 with second single lens 03 is the single lens of glass spherical mirror, double-cemented lens 04 also adopts glass spherical mirror, and glass cost is lower than aspheric glass, is favorable to the production and processing more.
Further, the light source 01 is a laser, and the wavelength band of the laser comprises 532nm or 638 nm.
Further, the first plane mirror is movable.
Further, the first single lens 02, the second single lens 03 and the double cemented lens 04 form a super-resolution collimating module, the central axes of the light source 01 and the super-resolution collimating module are both parallel to the X axis of a spatial coordinate system, and the first plane mirror is placed at a preset angle with respect to the X axis;
the dichroic mirror 06 is placed in parallel with the first plane mirror, the second plane mirror 08 is placed at the preset angle with respect to the Z axis of the spatial coordinate system, and the central axis of the super-resolution cylindrical mirror 09 is parallel to the Z axis.
Further, the preset angle is 45 ° or 135 °.
In this embodiment, super-resolution collimation module belongs to the design of independently developing, and this system avoids using aspherical mirror, and all adopts glass spherical mirror to accomplish, through the combination of single lens and two cemented lens, has realized the collimation of visible light scope and has expanded the beam function to glass cost is lower than aspherical glass, is favorable to the production and processing more. The whole super-resolution microscope system is simple in light path setting and low in cost.
The above-mentioned serial numbers of the embodiments of the present invention are merely for description and do not represent the merits of the embodiments. In the unit claims enumerating several means, several of these means may be embodied by one and the same item of hardware. The use of the words first, second, third and the like do not denote any order, but rather the words first, second and the like may be interpreted as indicating any order.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (6)
1. A super-resolution microscope system, comprising:
the light source is sequentially provided with a first single lens, a second single lens, a double cemented lens, a spatial light processing module, a dichroic mirror and a microscope objective lens from a sample; the first single lens and the second single lens are both single lenses of a glass spherical mirror; the spatial light processing module comprises a spatial light modulator and a first plane mirror;
the light source emits laser, the first single lens and the second single lens expand the laser, the double cemented lens collimates the expanded light beam, the spatial light modulator collimates the collimated expanded light beam after direct expansion, the first plane mirror reflects the modulated light to the dichroic mirror, and the dichroic mirror reflects the modulated light to the microscope objective;
the microscope objective converges the modulated light on an objective focal plane to excite a sample dyed by fluorescence and to excite fluorescence of a specific waveband;
the microscope objective, the dichroic mirror, the second plane reflector and the super-resolution cylindrical mirror are sequentially arranged from the sample to the super-resolution image surface;
the dichroic mirror passes the fluorescence of the specific wavelength band, and the second plane mirror reflects the fluorescence of the specific wavelength band passing through the dichroic mirror to the super-resolution cylindrical mirror;
and the super-resolution cylindrical lens converges the fluorescence of the specific wave band and images at the super-resolution image surface.
2. The super resolution microscope system according to claim 1, wherein the double cemented lens comprises three faces, two of which are concave and one of which is convex; and the double cemented lens is a double cemented lens of a glass spherical mirror.
3. The super resolution microscope system according to claim 1, wherein the light source is a laser, and the wavelength band of the laser comprises 532nm or 638 nm.
4. The super resolution microscope system of claim 1, wherein the first planar mirror is movable.
5. The super-resolution microscope system according to any one of claims 1 to 4, wherein the first single lens, the second single lens and the double cemented lens form a super-resolution collimating module, the central axes of the light source and the super-resolution collimating module are parallel to an X-axis of a spatial coordinate system, and the first plane mirror is disposed at a predetermined angle with respect to the X-axis;
the dichroic mirror is placed in parallel with the first plane reflecting mirror, the second plane reflecting mirror is placed at the preset angle with respect to the Z axis of the space coordinate system, and the central axis of the super-resolution cylindrical mirror is parallel to the Z axis.
6. The super resolution microscope system according to claim 5, wherein the preset angle is 45 ° or 135 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122348797.2U CN216387550U (en) | 2021-09-27 | 2021-09-27 | Super-resolution microscope system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122348797.2U CN216387550U (en) | 2021-09-27 | 2021-09-27 | Super-resolution microscope system |
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| CN216387550U true CN216387550U (en) | 2022-04-26 |
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| CN202122348797.2U Active CN216387550U (en) | 2021-09-27 | 2021-09-27 | Super-resolution microscope system |
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2021
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