CN210155404U - Large-view-field scanning imaging structure, microscope and microprobe - Google Patents
Large-view-field scanning imaging structure, microscope and microprobe Download PDFInfo
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- CN210155404U CN210155404U CN201921248597.6U CN201921248597U CN210155404U CN 210155404 U CN210155404 U CN 210155404U CN 201921248597 U CN201921248597 U CN 201921248597U CN 210155404 U CN210155404 U CN 210155404U
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- 238000003384 imaging method Methods 0.000 title claims abstract description 34
- 230000005284 excitation Effects 0.000 claims description 6
- 230000000007 visual effect Effects 0.000 abstract description 5
- 230000003287 optical effect Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003331 infrared imaging Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
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Abstract
The utility model relates to a microscopic imaging technical field of optics, concretely relates to big visual field scanning imaging structure, microscope and microprobe, a big visual field scanning imaging structure, including objective and scanner, the back focal plane of objective is located outside the objective, and the scanner can adopt one or two. The utility model provides an among the prior art problem that objective price is expensive and the volume is huge among the big visual field imaging structure.
Description
Technical Field
The invention relates to the technical field of optical microscopic imaging, in particular to a large-field scanning imaging structure, a microscope and a microprobe.
Background
The conventional microscope objective is an optical system with a large aperture angle and a small field angle, and since the microscope objective is used to provide an extremely high resolution (about 200nm, regardless of super resolution technology), its magnification is high, resulting in a small field angle.
With the development of neuroscience in recent years, the narrow field of view of the traditional multiphoton microscope cannot meet the requirement on functional imaging of massive nerve cells, and some research groups successively realize the multiphoton microscope with an ultra-large field of view.
In 2001 Martin Oheim et al ("Two-photon microscopy in broad tissue"; Journal of neuroscience methods.111:29-37.) compare the performance of high magnification objective and low magnification objective of approximate numerical aperture, indicating that increasing the aperture in front of the objective has a great benefit in increasing the collection efficiency of fluorescence, especially for deep multiphoton fluorescence excitation.
In 2015, the U.S. Pat. No. ("Ultra-large field-of-view two-photon microscopy", Opt express.2015Jun 1; 23(11): 13833-.
In 2016 ("A novel optical microscope for imaging large lenses and tissue volumes with sub-cellular resolution through", eLife 2016; 5: e18659.), confocal and nonlinear imaging of 6mm wide, 3mm thick samples was achieved using an incident beam of 30mm diameter, a scanner and a large objective lens of 63mm diameter.
These high numerical aperture objectives, while offering the advantages of large field of view, large throughput by increasing the beam diameter, result in objectives that are very expensive and bulky.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a big visual field scanning image structure to the problem that objective is expensive and the volume is huge among the big visual field image structure among the solution prior art.
In order to achieve the above object, the utility model provides a following technical scheme:
a large-field scanning imaging structure comprises an objective lens and a scanner, wherein the back focal plane of the objective lens is positioned outside the objective lens, and one or two scanners can be adopted.
The principle and the beneficial effect of the scheme are as follows:
the objective lens is used to focus the excitation beam reflected by the scanner in the sample and collect the emitted light signal excited in the sample. In the traditional laser scanning microscope, a scanning lens and a sleeve lens must be arranged between a scanner and an objective lens, so that the length of a scanning and imaging optical path of the microscope is long. In the scheme, the back focal plane of the objective lens is far away from the lens body (usually several millimeters away), so that a scanner is installed in enough space, a scanning lens and a sleeve lens do not need to be installed at the moment, and the length of a scanning and imaging light path of the microscope is greatly shortened. Compared with a common scanning lens, the scheme has the advantages of ultra-short focal length, high numerical aperture and smaller field angle; compared with the common microscope objective lens, the scheme has a larger field angle and an external back focal plane. Therefore, the scheme is very different from a common scanning lens and a common microscope objective lens and is unique.
Further, when the number of scanners is one, the scanner is located at the back focal plane of the objective lens. In the process of practical application, the scheme can adopt one scanner, and the scanner is positioned at the back focal plane of the objective lens at the moment, so that large-field scanning is realized under the condition of keeping the volume smaller.
Further, when the number of scanners is two, the back focal plane of the objective lens is located between the two scanners. In the process of practical application, two scanners can be adopted in the scheme, the back focal plane of the objective lens is positioned between the two scanners at the moment, and the size of the imaging structure is small.
Further, the back focal plane of the objective lens is located at the middle position of the two scanners. The back focal plane of the objective lens is positioned in the middle of the two scanners, and the practical application effect is better under the condition.
Further, the back focal plane is calculated according to the wavelength of the exciting light. The calculation is convenient to operate, and the result is accurate.
Further, the wavelength of excitation light used was 920 nm and the wavelength of emission light was 520 nm. Under the imaging condition, the scanning imaging with a large field of view can be achieved.
Further, the objective lens comprises a first lens, a second lens, a third lens, a fourth lens and a fifth lens which are arranged in sequence from the image side to the object side. The objective lens of the scheme is small in size, comprises five lenses, and is good in scanning imaging effect and high in integration level.
Furthermore, the numerical aperture of the objective lens in the microscope using the large-field scanning imaging structure is more than or equal to 0.7 mm. The arrangement can improve the collection efficiency of fluorescence.
Further, the field angle of the microscope is 15 degrees or more, and the field diameter is 1.65 mm. The field angle and the field diameter can still meet the requirement of large field under the condition of keeping the volume small, and the requirement of the existing functional imaging can be met.
Further, the diameter of incident light adopted by the microscope probe applying the large-field scanning imaging structure is 5 mm. The diameter of the incident light is much smaller than that of the light beam in the prior art, thus enabling the volume of the objective lens to be greatly reduced.
Drawings
Fig. 1 is a schematic view of an optical structure according to a first embodiment of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the device comprises a first lens 1, a second lens 2, a third lens 3, a fourth lens 4, a fifth lens 5 and a scanner 6.
The left side in fig. 1 is the image side, and the right side is the object side; the image side refers to the side close to the imaging end, and the object side refers to the side close to the object.
Example one
As shown in fig. 1, a large field of view scanning imaging structure includes an objective lens and a scanner 6, wherein the objective lens includes a first lens 1, a second lens 2, a third lens 3, a fourth lens 4, and a fifth lens 5, which are arranged in order from an image side to an object side. The first lens 1 is provided with a first left spherical surface on the left side and a first right spherical surface on the right side; the second lens 2 has a second left spherical surface on the left side and a second right spherical surface on the right side; the third lens 3 has a third left spherical surface on the left side and a third right spherical surface on the right side; the fourth lens 4 has a fourth left spherical surface on the left side and a fourth right spherical surface on the right side; the fifth lens 5 has a fifth left spherical surface on the left side and a fifth right spherical surface on the right side. The first lens 1, the second lens 2, the third lens 3, the fourth lens 4 and the fifth lens 5 are all made of optical glass or high molecular polymer or infrared imaging materials.
The back focal plane of the objective lens is located outside the objective lens, and in this embodiment, the back focal plane of the objective lens is calculated according to the wavelength of the excitation light. One or two scanners 6 may be used, in particular, when there is one scanner 6, the scanner 6 is located at the back focal plane of the objective lens.
In practical use, the diameter of the incident light is 5 mm, the wavelength of the excitation light is 920 nm, and the wavelength of the emission light is 520 nm. The numerical aperture of the objective is more than or equal to 0.7 mm, the field angle of the microscope is more than or equal to 15 degrees, the diameter of the field is 1.65 mm, the objective is equivalent to a Nikon 15 magnification microscope objective and is compatible with a scanner 6 of a commercial desk-top laser scanning microscope. The length of the shell of the objective lens can be controlled to be 29 mm, and the diameter of the shell of the objective lens can be controlled to be 16 mm, so that the objective lens is low in cost, small in size and very suitable for two-photon imaging of living nervous tissue. The large-view-field scanning imaging structure can be applied to instruments such as a microscope, a microprobe and the like.
Example two
The present embodiment differs from the first embodiment in that there are two scanners 6, and the back focal plane of the objective lens is located between the two scanners 6.
EXAMPLE III
The present embodiment is different from the first embodiment in that there are two scanners 6, and the back focal plane of the objective lens is located at the middle position of the two scanners 6.
The above description is only an example of the present invention, and the common general knowledge of the known specific structures and characteristics of the embodiments is not described herein. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. A large field of view scanning imaging structure, its characterized in that: including an objective lens with its back focal plane located outside the objective lens, and a scanner, which may employ one or both.
2. The large field of view scanning imaging architecture of claim 1, wherein: the number of scanners is one, and the scanner is located at the back focal plane of the objective lens.
3. The large field of view scanning imaging architecture of claim 1, wherein: the number of scanners is two, and the back focal plane of the objective lens is located between the two scanners.
4. A large field of view scanning imaging architecture as claimed in claim 3, wherein: the back focal plane of the objective lens is located at the middle position of the two scanners.
5. A large field of view scanning imaging arrangement according to any one of claims 1-4, wherein: the back focal plane is calculated according to the wavelength of the exciting light.
6. The large field of view scanning imaging architecture of claim 5, wherein: the wavelength of the excitation light used was 920 nm and the wavelength of the emission light was 520 nm.
7. The large field of view scanning imaging architecture of claim 6, wherein: the objective lens comprises a first lens, a second lens, a third lens, a fourth lens and a fifth lens which are arranged in sequence from the image side to the object side.
8. A microscope employing the large field of view scanning imaging architecture of claim 7, wherein: the numerical aperture of the objective lens is greater than or equal to 0.7 mm.
9. The microscope of claim 8, wherein: the field angle is more than or equal to 15 degrees, and the field diameter is 1.65 millimeters.
10. A microprobe using the large field of view scanning imaging architecture of claim 7, wherein: the incident light used has a diameter of 5 mm.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN2019100633327 | 2019-01-23 |
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| CN210155404U true CN210155404U (en) | 2020-03-17 |
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| CN201921247897.2U Expired - Fee Related CN210572987U (en) | 2019-01-23 | 2019-08-02 | Large-view-field miniature endoscope |
| CN201921248597.6U Expired - Fee Related CN210155404U (en) | 2019-01-23 | 2019-08-02 | Large-view-field scanning imaging structure, microscope and microprobe |
| CN201921247899.1U Expired - Fee Related CN210155401U (en) | 2019-01-23 | 2019-08-02 | Large-field microscope objective lens |
| CN201910713390.XA Pending CN111474695A (en) | 2019-01-23 | 2019-08-02 | Large-field microscope objective lens |
| CN201921248382.4U Expired - Fee Related CN210166557U (en) | 2019-01-23 | 2019-08-02 | Large-view-field imaging lens |
| CN201910717807.XA Pending CN111474696A (en) | 2019-01-23 | 2019-08-05 | Large-view-field head-mounted microscope |
| CN201921256740.6U Expired - Fee Related CN210155407U (en) | 2019-01-23 | 2019-08-05 | Large-view-field head-mounted microscope |
| CN201911288473.5A Pending CN110794565A (en) | 2019-01-23 | 2019-12-11 | Large-view-field handheld microscope |
| CN201922220396.1U Active CN211086790U (en) | 2019-01-23 | 2019-12-11 | Large-view-field handheld microscope |
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| CN201910713367.0A Pending CN111474694A (en) | 2019-01-23 | 2019-08-02 | Large-view-field miniature endoscope |
| CN201921247897.2U Expired - Fee Related CN210572987U (en) | 2019-01-23 | 2019-08-02 | Large-view-field miniature endoscope |
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| CN201921247899.1U Expired - Fee Related CN210155401U (en) | 2019-01-23 | 2019-08-02 | Large-field microscope objective lens |
| CN201910713390.XA Pending CN111474695A (en) | 2019-01-23 | 2019-08-02 | Large-field microscope objective lens |
| CN201921248382.4U Expired - Fee Related CN210166557U (en) | 2019-01-23 | 2019-08-02 | Large-view-field imaging lens |
| CN201910717807.XA Pending CN111474696A (en) | 2019-01-23 | 2019-08-05 | Large-view-field head-mounted microscope |
| CN201921256740.6U Expired - Fee Related CN210155407U (en) | 2019-01-23 | 2019-08-05 | Large-view-field head-mounted microscope |
| CN201911288473.5A Pending CN110794565A (en) | 2019-01-23 | 2019-12-11 | Large-view-field handheld microscope |
| CN201922220396.1U Active CN211086790U (en) | 2019-01-23 | 2019-12-11 | Large-view-field handheld microscope |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113189758A (en) * | 2021-03-31 | 2021-07-30 | 苏州溢博伦光电仪器有限公司 | Microscope objective |
| CN113189741A (en) * | 2021-03-31 | 2021-07-30 | 苏州溢博伦光电仪器有限公司 | High numerical aperture scanning lens |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111474694A (en) * | 2019-01-23 | 2020-07-31 | 苏州溢博伦光电仪器有限公司 | Large-view-field miniature endoscope |
| CN111638594A (en) * | 2020-05-27 | 2020-09-08 | 南方科技大学 | Optical system |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107991769B (en) * | 2018-01-12 | 2020-07-10 | 凝辉(天津)科技有限责任公司 | Two-dimensional scanning device |
| CN111474694A (en) * | 2019-01-23 | 2020-07-31 | 苏州溢博伦光电仪器有限公司 | Large-view-field miniature endoscope |
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2019
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- 2019-08-02 CN CN201921247897.2U patent/CN210572987U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201921248597.6U patent/CN210155404U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201921247899.1U patent/CN210155401U/en not_active Expired - Fee Related
- 2019-08-02 CN CN201910713390.XA patent/CN111474695A/en active Pending
- 2019-08-02 CN CN201921248382.4U patent/CN210166557U/en not_active Expired - Fee Related
- 2019-08-05 CN CN201910717807.XA patent/CN111474696A/en active Pending
- 2019-08-05 CN CN201921256740.6U patent/CN210155407U/en not_active Expired - Fee Related
- 2019-12-11 CN CN201911288473.5A patent/CN110794565A/en active Pending
- 2019-12-11 CN CN201922220396.1U patent/CN211086790U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113189758A (en) * | 2021-03-31 | 2021-07-30 | 苏州溢博伦光电仪器有限公司 | Microscope objective |
| CN113189741A (en) * | 2021-03-31 | 2021-07-30 | 苏州溢博伦光电仪器有限公司 | High numerical aperture scanning lens |
Also Published As
| Publication number | Publication date |
|---|---|
| CN210166557U (en) | 2020-03-20 |
| CN210155407U (en) | 2020-03-17 |
| CN111474696A (en) | 2020-07-31 |
| CN111474695A (en) | 2020-07-31 |
| CN211086790U (en) | 2020-07-24 |
| CN210155401U (en) | 2020-03-17 |
| CN110794565A (en) | 2020-02-14 |
| CN111474694A (en) | 2020-07-31 |
| CN210572987U (en) | 2020-05-19 |
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