CN216350739U

CN216350739U - Kit for ROS and FH joint detection

Info

Publication number: CN216350739U
Application number: CN202123069875.1U
Authority: CN
Inventors: 黄一跃
Original assignee: Shenzhen Yuemei Biomedical Technology Co ltd
Current assignee: Shenzhen Yuemei Biomedical Technology Co ltd
Priority date: 2021-12-08
Filing date: 2021-12-08
Publication date: 2022-04-19
Anticipated expiration: 2031-12-08

Abstract

The utility model discloses a kit for combined detection of ROS and FH, which comprises a kit and a sampling box, wherein the kit comprises a shell and a bottom plate, the shell and the bottom plate are integrally formed by injection molding, the inner cavity of the shell is connected with a plastic reaction cup I, a plastic reaction cup II and a plastic reaction cup III in an adhesive manner, and the tops of the plastic reaction cup I, the plastic reaction cup II and the plastic reaction cup III are communicated with connecting pipes. The utility model realizes the effects of convenient operation and accurate test under the matching use of the sampling box, the plastic reaction cup I, the plastic reaction cup II, the plastic reaction cup III, the external interface I, the external thread, the reagent box cover and the external interface II.

Description

Kit for ROS and FH joint detection

Technical Field

The utility model belongs to the technical field of medical detection equipment, and particularly relates to a kit for combined detection of ROS and FH.

Background

(Reactive Oxygen Species, ROS) ROS is a short name for Reactive Oxygen Species, the Reactive Oxygen Species are generated by aerobic cells in a metabolic process, when the Reactive Oxygen Species are accumulated too much, the cells are damaged, and an oxidative stress organism has a set of antioxidant stress system of the organism to protect the cells from being damaged. With the continuous and deep research of active oxygen, its important role in the process of tumorigenesis and development has been confirmed, and the imbalance between active oxygen and antioxidant stress system in vivo is a key factor of tumor formation.

(cell free bacteria Protoporphyrin FH) is called FH substance for short, is an effective biomarker generated in the process of cell metabolism malignant change, and the content of FH substance in exfoliated epithelial Cells is gradually increased in the process of conversion from precancerous lesion to in-situ cancer, so that early cancer screening is feasible by measuring the FH substance in the epithelial Cells. Qualitative research of FH materials in tumor cells has been carried out in screening experiments on partial malignant tumors, and tumors are screened mainly by developing color based on the oxidation-reduction reaction of FH materials with specific materials and determining the amount of FH material content based on the result of the color reaction.

The change of the content of ROS and FH is closely and positively correlated with abnormal cell lesions, and the detection of the two substances can better show precancerous lesions. The abnormal content of ROS and FH is the result of abnormal biochemical reaction of cells, and can be used as biochemical marker of abnormal pathological changes of cells, so that the detection reagent developed based on the principle has the advantages of simplicity, convenience, rapidness, low price, high accuracy, early detection and the like.

In order to solve the problems that a few single ROS or HF substances on the market have false positive and false negative detection problems, the detection steps are complex and complicated, and the working efficiency is low, the utility model provides a kit for ROS and FH combined detection, which is used for improving the functional characteristics of R0S or FH test reagents.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a kit for combined detection of ROS and FH, which aims to solve the technical problems in the background technology.

The technical scheme for solving the technical problems is as follows: a kit for ROS and FH joint detection comprises a kit and a sampling box, wherein the kit comprises a shell and a bottom plate, the shell and the bottom plate are integrally formed through injection molding, the inner cavity of the shell is connected with a first plastic reaction cup, a second plastic reaction cup and a third plastic reaction cup in a splicing manner, the tops of the first plastic reaction cup, the second plastic reaction cup and the third plastic reaction cup are communicated with a connecting pipe, the top of the shell is bonded with a first three external connectors and communicated with the connecting pipe, the surface of the first external connector is provided with external threads, the first external connector is connected with a reagent box cover through the external threads, the external thread pitch of the first external connector surface on the rightmost side is smaller than the thread pitch of the other two external threads, the top of the sampling box is communicated with a second external connector, the thread pitch of the second external connector surface is larger than the thread pitch of the external threads on the leftmost side, the right side of bottom plate has seted up the mounting groove, the mounting groove uses with the sampling box cooperation.

The utility model adopts the above, further: and an inner cavity of the first plastic reaction cup is provided with ROS reaction liquid.

The utility model adopts the above, further: and an inner cavity of the plastic reaction cup II is provided with FH reaction liquid.

The utility model adopts the above, further: and a color developing liquid is arranged in the inner cavity of the plastic reaction cup III.

The utility model adopts the above, further: notches are formed in the bottom of the bottom plate and distributed in an array mode.

The utility model adopts the above, further: the shape of mounting groove is circular, and the inner wall bonding of mounting groove has elastic rubber circle.

The utility model has the beneficial effects that:

the utility model is made by adopting integral injection molding, integrates the ROS and FH material inspection, and sets external threads with different intervals, so that the force and the turns of unscrewing the reagent box cover are different for a user, the user can operate in sequence according to the detection sequence, the condition of experimental misoperation is avoided, under the matched use of the sampling box, the plastic reaction cup I, the plastic reaction cup II, the plastic reaction cup III, the external interface I, the external threads, the reagent box cover and the external interface II, the convenient operation and the accurate test effect are realized, in the practical use, the carrying and the experiment can be convenient, a plurality of groups of the reagent boxes can be placed at one position, the reference comparison of the detection result data is carried out, the induction, the arrangement and the summarization of research and development data are facilitated, the iteration and the optimization in the future are facilitated, and the problem that the false positive and the false negative exist in the inspection of a few independent ROS or HF materials in the current market is solved, the detection steps are complex and tedious, and the working efficiency is low.

Drawings

The above and/or other advantages of the utility model will become more apparent and more readily appreciated from the following detailed description taken in conjunction with the accompanying drawings, which are given by way of illustration only and not by way of limitation, and in which:

FIG. 1 is a schematic front view of an embodiment of a kit for combined detection of ROS and FH in accordance with the present invention;

FIG. 2 is a schematic sectional view of a front view of an embodiment of the kit for combined detection of ROS and FH of the present invention;

FIG. 3 is a schematic top view of a partial structure of an embodiment of the kit for combined detection of ROS and FH of the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

1. a kit; 101. a housing; 102. a base plate; 2. a sample cartridge; 3. a first plastic reaction cup; 4. a plastic reaction cup II, 5 and a plastic reaction cup III; 6. a connecting pipe; 7. an external interface I; 8. an external thread; 9. a reagent box cover; 10. a second external interface; 11. mounting grooves; 12. a recess.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The drawings in the present specification are schematic views to assist in explaining the concept of the present invention, and schematically show the shapes of respective portions and their mutual relationships. It is noted that the drawings are not necessarily to the same scale so as to clearly illustrate the structures of the various elements of the embodiments of the utility model. Like reference numerals are used to denote like parts.

FIG. 1-3 show a kit for ROS and FH combined detection of the present invention, comprising a kit 1 and a sampling box 2, wherein the kit 1 comprises a housing 101 and a base plate 102, the housing 101 and the base plate 102 are integrally formed by injection molding, a first plastic reaction cup 3, a second plastic reaction cup 4 and a third plastic reaction cup 5 are glued to the inner cavity of the housing 101, the tops of the first plastic reaction cup 3, the second plastic reaction cup 4 and the third plastic reaction cup 5 are all communicated with a connecting pipe 6, three first external connectors 7 are glued to the top of the housing 101, the first external connectors 7 are communicated with the connecting pipe 6, external threads 8 are arranged on the surfaces of the first external connectors 7, the first external connectors 7 are connected with a reagent box cover 9 by the external threads 8 through threads, the thread pitch of the external threads 8 on the surface of the first external connector 7 located at the rightmost side is smaller than the thread pitch of the other two external threads 8, the top of the sampling box 2 is communicated with a second external connector 10, the screw thread interval on two 10 surfaces of external tapping is greater than the screw thread interval that is located leftmost external screw thread 8, mounting groove 11 has been seted up on bottom plate 102's right side, mounting groove 11 uses with the cooperation of sampling box 2, the shape of mounting groove 11 is circular, and the inner wall of mounting groove 11 bonds and has the elastic rubber circle, through the setting of elastic rubber circle, be favorable to crowding sampling box 2 into mounting groove 11 in, notch 12 has been seted up to bottom plate 102's bottom, notch 12 is the array and distributes.

Furthermore, an inner cavity of the first plastic reaction cup 3 is provided with ROS reaction liquid.

Furthermore, the inner cavity of the plastic reaction cup II 4 is provided with FH reaction liquid.

Furthermore, the inner cavity of the plastic reaction cup III 5 is provided with color developing liquid.

The utility model also discloses a detection method of the kit for combined detection of ROS and FH, which comprises the following steps:

firstly, sampling a patient by using a disposable sampling swab;

step two, putting the sampling swab into the sample preservation solution in the sampling box 2 and stirring uniformly;

step three, turning off a plastic reaction cup I3;

step four, respectively adding and dripping sample storage liquid sucked by a disposable dropper into a kit containing ROS and FH reaction liquid contained in a plastic reaction cup I3 and a plastic reaction cup II 4;

and fifthly, absorbing the color development liquid in the sampling box 2 by using a disposable dropper, respectively dripping the color development liquid into a plastic reaction cup I3 and a plastic reaction cup II 4, so that the color development liquid respectively reacts with ROS and FH reaction chambers for about three minutes, and distinguishing the shade and the shade of the cancer according to the reaction color development.

The technical features disclosed above are not limited to the combinations with other features disclosed, and other combinations between the technical features can be performed by those skilled in the art according to the purpose of the utility model, so as to achieve the purpose of the utility model.

Claims

1. The utility model provides a ROS and FH joint detection use kit, includes kit (1) and sampling box (2), its characterized in that, kit (1) includes shell (101) and bottom plate (102), shell (101) and bottom plate (102) are through moulding plastics integrated into one piece, the inner chamber of shell (101) is glued to have plastic reaction cup one (3), plastic reaction cup two (4) and plastic reaction cup three (5), the top of plastic reaction cup one (3), plastic reaction cup two (4) and plastic reaction cup three (5) all communicates with connecting pipe (6), the top of shell (101) bonds and has three external tapping one (7), and external tapping one (7) and connecting pipe (6) are linked together, the surface of external tapping one (7) is equipped with external screw thread (8), external tapping one (7) have reagent lid (9) through external screw thread (8) threaded connection, be located the rightmost side external screw thread (8) the screw thread interval on external tapping (7) surface is less than the screw thread interval of two other external screw threads (8), the top intercommunication of sampling box (2) has external tapping two (10), the screw thread interval on external tapping two (10) surfaces is greater than and is located the leftmost the screw thread interval of external screw thread (8), mounting groove (11) have been seted up on the right side of bottom plate (102), mounting groove (11) are used with sampling box (2) cooperation.

2. The kit for combined detection of ROS and FH according to claim 1, wherein an ROS reaction solution is provided in the inner cavity of said plastic reaction cup I (3).

3. The kit for combined detection of ROS and FH according to claim 1, wherein an FH reaction solution is disposed in an inner cavity of said plastic reaction cup two (4).

4. The kit for combined detection of ROS and FH according to claim 1, wherein a color developing solution is provided in the inner cavity of said plastic reaction cup III (5).

5. The kit for detecting ROS and FH in combination with a reagent kit according to claim 1, wherein said bottom plate (102) is provided with notches (12) at the bottom thereof, and said notches (12) are arranged in an array.

6. The kit for combined detection of ROS and FH according to claim 1, wherein said mounting groove (11) is circular in shape, and an elastic rubber ring is bonded to the inner wall of the mounting groove (11).