CN216309796U

CN216309796U - Fluorescent imaging device capable of rapidly switching optical module

Info

Publication number: CN216309796U
Application number: CN202122553548.7U
Authority: CN
Inventors: 李坤
Original assignee: Beijing Haiweier Technology Development Co ltd
Current assignee: Beijing Haiweier Technology Development Co ltd
Priority date: 2021-10-22
Filing date: 2021-10-22
Publication date: 2022-04-15
Anticipated expiration: 2031-10-22

Abstract

The utility model discloses a fluorescence imaging device capable of quickly switching optical modules, which comprises an optical module, wherein the optical module comprises a laser, a collimating lens, a dichroic mirror and a photoelectric detector, the laser is arranged on one side inside the optical module, the collimating lens is arranged inside the laser, the dichroic mirror is arranged on one side of the laser, the photoelectric detector is arranged below the dichroic mirror and connected with a data processing system through an electric signal, a pair of positioning holes are formed in one side of the surface of the optical module, a locking screw is fixedly arranged on the other side of the surface of the optical module, the optical module is clamped and installed in an optical module fixing seat, a pair of positioning pins are installed on the inner wall of the optical module fixing seat, and a locking nut is installed on the outer wall of one side of the surface of the optical module fixing seat.

Description

Fluorescent imaging device capable of rapidly switching optical module

Technical Field

The utility model relates to the technical field of optics, in particular to a fluorescence imaging device capable of quickly switching optical modules.

Background

Fluorescent molecular imaging is a novel molecular imaging technology which is rapidly developed in the field of biomedicine, and non-invasive detection on the cellular and molecular level is realized by implanting specific fluorescent molecular probes into tissues. The fluorescence molecular imaging has the characteristics of high sensitivity, high imaging speed, good repeatability, low experimental cost, easy and practical imaging of both living bodies and in vitro, and the like, and has extremely wide development prospect.

The conventional fluorescence imaging equipment on the market at present has a small number of optical channels, and only limited fluorescence information can be obtained each time; when the optical module is replaced, the operation is complex and time-consuming, and the operation must be carried out by professional personnel. Due to the small difference of the sizes of mechanical parts of different optical modules and the chromatic aberration and aberration of the objective lens, the optical module is out of focus after being replaced, and the equipment needs to be focused again. These deficiencies greatly affect the efficiency of the assay and we therefore improve on this by providing a fluorescence imaging device that can rapidly switch optical modules.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problems, the utility model provides the following technical scheme:

the utility model relates to a fluorescence imaging device capable of rapidly switching an optical module, which comprises the optical module, wherein the optical module comprises a laser, a collimating lens, a dichroic mirror and a photoelectric detector, a laser is arranged on one side inside the optical module, a collimating lens is arranged inside the laser, a dichroic mirror is arranged and installed on one side of the laser, a photoelectric detector is arranged and installed below the dichroic mirror, the photoelectric detector is connected with the data processing system through an electric signal, one side of the surface of the optical module is provided with a pair of positioning holes, and the other side of the surface of the optical module is fixedly provided with a locking screw, the optical module is clamped and arranged in the optical module fixing seat, a pair of positioning pins is installed on the inner wall of the optical module fixing seat, and a locking nut is installed on the outer wall of one side of the surface of the optical module fixing seat.

The utility model relates to a fluorescence imaging device capable of rapidly switching optical modules, which comprises at least one set of optical module for exciting and collecting fluorescence, an optical module fixing seat for fixing the optical module, a carrying glass for carrying a sample to be tested, a movable objective lens for focusing exciting light and collecting fluorescence, and a data processing system for controlling movement and processing fluorescence signals

The utility model relates to a fluorescence imaging device capable of quickly switching optical modules, which comprises a positioning hole formed in one side of the surface of an optical module and a positioning pin fixedly installed in an optical module fixing seat, wherein the positioning hole is matched with the positioning pin, and a locking nut is matched with a locking screw.

The utility model relates to a fluorescence imaging device capable of rapidly switching optical modules, which comprises a positioning pin, wherein the positioning precision of the positioning pin is higher than 0.03 mm.

The fluorescence imaging device capable of rapidly switching the optical module comprises the optical module, wherein the wavelength range of exciting light of the optical module is 400-1100nm, the laser emitting angles of lasers with different wavelengths of the optical module are consistent, and the positions of focuses of the exciting light emitted by the lasers with different wavelengths of the optical module are consistent after the laser emitting angles are focused by an objective lens.

The utility model relates to a fluorescence imaging device capable of rapidly switching optical modules, which comprises at most three groups of optical modules and optical module fixing seats, wherein the consistency of the laser emergent angles and the focal positions of the optical modules is calibrated through a calibration tool.

The utility model relates to a fluorescence imaging device capable of rapidly switching optical modules, which comprises a calibration tool, an optical module fixing seat, a spectroscope, an observation screen, an objective lens, color paper and a stepping motor, wherein the objective lens is arranged on the reflection side of the spectroscope, the observation screen is arranged on the transmission side of the spectroscope, the stepping motor is arranged on one side of the objective lens, the color paper is arranged on the stepping motor, and the objective lens adopt the same type of objective lens.

The utility model has the beneficial effects that:

the fluorescent imaging device capable of rapidly switching the optical modules ensures the consistency of the emergent light angle and the focus position of each optical module through the calibration tool; the consistency of the assembly precision of each optical module and each fixed seat of the imaging device is ensured through the positioning pin structure. Therefore, the optical module is quickly replaced, equipment does not need to be focused again, and the test efficiency is greatly improved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model. In the drawings:

FIG. 1 is a schematic diagram of a fluorescence imaging apparatus capable of rapidly switching optical modules according to the present invention;

FIG. 2 is a diagram of an internal structure of an optical module of a fluorescence imaging apparatus capable of rapidly switching the optical module according to the present invention;

FIG. 3 is a diagram of an external structure of an optical module of a fluorescence imaging apparatus capable of fast switching the optical module according to the present invention;

FIG. 4 is a diagram of a fixing base of an optical module of a fluorescence imaging device capable of switching optical modules rapidly according to the present invention;

FIG. 5 is a schematic diagram of a calibration fixture of a fluorescence imaging apparatus capable of rapidly switching optical modules according to the present invention.

In the figure: 1. an optical module; 2. an optical module holder; 3. a movable objective lens; 4. carrying glass; 5. a sample to be tested; 6. exciting light; 7. fluorescence; 8. a data processing system; 9. a tool fixing seat; 10. a beam splitter; 11. a viewing screen; 12. an objective lens; 13. color paper; 14. a stepping motor; 101. a laser; 102. a collimating lens; 103. a dichroic mirror; 104. a photodetector; 105. positioning holes; 106. locking the screw; 201. locking the nut; 202. and a positioning pin.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

Example (b): as shown in fig. 1-5, the fluorescence imaging apparatus capable of switching optical modules rapidly according to the present invention includes an optical module 1, where the optical module 1 includes a laser 101, a collimating lens 102, a dichroic mirror 103, and a photodetector 104, one side inside the optical module 1 is provided with the laser 101, the inside of the laser 101 is provided with the collimating lens 102, one side of the laser 101 is provided with the dichroic mirror 103, the lower side of the dichroic mirror 103 is provided with the photodetector 104, and the photodetector 104 is connected to a data processing system 8 through an electrical signal, one side of the surface of the optical module 1 is provided with a pair of positioning holes 105, the other side of the surface of the optical module 1 is fixedly provided with a locking screw 106, the optical module 1 is mounted in an optical module fixing base 2 in a snap-fit manner, the inner wall of the optical module fixing base 2 is provided with a pair of positioning pins 202, and a locking nut 201 is arranged and installed on the outer wall of one side of the surface of the optical module fixing seat 2.

The device comprises at least one set of optical module 1 for exciting and collecting fluorescence, an optical module fixing seat 2 for fixing the optical module, carrier glass 4 for bearing a sample to be tested, a movable objective lens 3 for focusing exciting light 6 and collecting fluorescence 7, and a data processing system 8 for controlling motion and processing fluorescence signals

The positioning hole 105 formed on one side of the surface of the optical module 1 is matched with the positioning pin 202 fixedly installed inside the optical module fixing base 2, and the locking nut 201 is matched with the locking screw 106.

Wherein, the positioning accuracy of the positioning pin 202 is higher than 0.03 mm.

The wavelength range of the excitation light of the optical module 1 is 400-1100nm, the laser emitting angles of the lasers 101 with different wavelengths of the optical module 1 are the same, and the focal positions of the excitation light 6 emitted by the lasers 101 with different wavelengths of the optical module 1 are the same after being focused by the objective lens 3.

The optical module 1 and the optical module fixing seat 2 are at most three groups, and the consistency of the laser emitting angle and the focus position of the optical module 1 is calibrated through a calibration tool.

Wherein, the calibration frock includes frock fixing base 9, spectroscope 10, observation screen 11, objective 12, look paper 13, step motor 14, spectroscope 10 reflection one side is provided with objective 12, and transmission one side is provided with observation screen 11, objective 12 one side is provided with step motor 14, be provided with look paper 13 on the step motor 14, and objective 12 with objective 3 adopts same model objective.

The working principle is as follows: when the device is used, the integrity of the device is checked firstly, laser is lightened after the device is confirmed to be correct, and excitation light emitted by the optical module 1 is divided into two beams of light through the spectroscope 10. One of the beams strikes a remote viewing screen 11 and the pitch of the laser 101 in the optical module 1 is adjusted so that the excitation light strikes a fixed location on the viewing screen 11. According to the same mode, the light emitted by all the optical modules can be emitted to the same position, and the angle consistency of the emergent light is ensured. The other beam of light is focused by the movable objective lens 3 and then strikes the color paper 13, the color paper 13 is fixed on the stepping motor 14, and the color paper 13 emits fluorescence after being excited by laser. The distance between the color paper 14 and the movable objective lens 3 is different, the excited fluorescence intensity is different, the stepping motor 11 is controlled to adjust the distance between the color paper 13 and the movable objective lens 3 from far to near or from near to far, so as to obtain fluorescence intensity curves under different distances, the maximum value of the curve is the focal position, the front and back distances of the collimating lens 102 in the laser 101 are adjusted, the divergence angle of the laser is further adjusted, the maximum value positions of the fluorescence intensity curves obtained by all optical modules are consistent, and the consistency of the focal lengths of all the optical modules is ensured.

Finally, it should be noted that: in the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The fluorescence imaging device capable of rapidly switching the optical module comprises an optical module (1) and is characterized in that the optical module (1) comprises a laser (101), a collimating lens (102), a dichroic mirror (103) and a photoelectric detector (104), the laser (101) is arranged on one side inside the optical module (1), the collimating lens (102) is arranged inside the laser (101), the dichroic mirror (103) is arranged on one side of the laser (101), the photoelectric detector (104) is arranged below the dichroic mirror (103), the photoelectric detector (104) is connected with a data processing system (8) through an electric signal, a pair of positioning holes (105) is formed in one side of the surface of the optical module (1), and a locking screw (106) is fixedly arranged on the other side of the surface of the optical module (1), optical module (1) block is installed in optical module fixing base (2), install a pair of locating pin (202) on optical module fixing base (2) inner wall, just set up on the outer wall of optical module fixing base (2) surface one side and install lock nut (201).

2. The fluorescence imaging device of claim 1, comprising at least one set of said optical module (1) for exciting and collecting fluorescence, an optical module holder (2) for holding said optical module, a carrying glass (4) for carrying a sample (5) to be measured, a movable objective lens (3) for focusing the excitation light (6) and collecting fluorescence (7), and a data processing system (8) for controlling the movement and processing the fluorescence signal.

3. The fluorescence imaging device of claim 1, wherein a positioning hole (105) formed on one side of the surface of the optical module (1) is matched with a positioning pin (202) fixedly installed inside the optical module fixing base (2), and the locking nut (201) is matched with the locking screw (106).

4. The fluorescence imaging apparatus of claim 3, wherein the positioning pin (202) has a positioning accuracy higher than 0.03 mm.

5. The fluorescence imaging device of claim 1, wherein the wavelength range of the excitation light of the optical module (1) is 400-1100nm, the emission angles of the lasers (101) with different wavelengths of the optical module (1) are the same, and the focal positions of the excitation light (6) emitted by the lasers (101) with different wavelengths of the optical module (1) are the same after being focused by the movable objective lens (3).

6. The fluorescence imaging device capable of rapidly switching the optical module according to claim 1, wherein the optical module (1) and the optical module holder (2) are at most three groups, and the consistency between the laser emitting angle and the focal position of the optical module (1) is calibrated by a calibration tool.

7. The calibration fixture according to claim 6, wherein the calibration fixture comprises a fixture fixing seat (9), a spectroscope (10), an observation screen (11), an objective lens (12), color paper (13) and a stepping motor (14), wherein the objective lens (12) is arranged on the reflection side of the spectroscope (10), the observation screen (11) is arranged on the transmission side of the spectroscope (10), the stepping motor (14) is arranged on one side of the objective lens (12), the color paper (13) is arranged on the stepping motor (14), and the objective lens (12) and the movable objective lens (3) are of the same type.