CN216295781U

CN216295781U - Protein purification dialysis device

Info

Publication number: CN216295781U
Application number: CN202122685955.3U
Authority: CN
Inventors: 杨福旺; 王润
Original assignee: Wuxi Shengherui Biotechnology Co ltd
Current assignee: Wuxi Shengherui Biotechnology Co ltd
Priority date: 2021-11-04
Filing date: 2021-11-04
Publication date: 2022-04-15
Anticipated expiration: 2031-11-04

Abstract

The utility model discloses a protein purification dialysis device, which comprises a barrel, an upper cover and a clamping rotating assembly, wherein the upper cover is arranged on the barrel, the clamping rotating assembly is arranged on the upper cover and arranged in the barrel, the clamping rotating assembly comprises a bearing, an inserting rod, a supporting rotating shaft, a rotating motor and a clamping supporting assembly, the bearing is arranged on the bottom wall in the barrel, the inserting rod is arranged on the bearing, the rotating motor is arranged at the upper end of the upper cover, one end of the supporting rotating shaft is arranged at the output end of the rotating motor, the other end of the supporting rotating shaft penetrates through the upper cover and is arranged in the barrel, the other end of the supporting rotating shaft is sleeved on the inserting rod, and the clamping supporting assembly is arranged on the supporting rotating shaft. The utility model belongs to the technical field of protein purification dialysis, and particularly relates to a protein purification dialysis device which can stably separate and clamp a plurality of dialysis bags, can stir buffer solution and ensure the effect of protein purification dialysis.

Description

Protein purification dialysis device

Technical Field

The utility model belongs to the technical field of protein purification dialysis, and particularly relates to a protein purification dialysis device.

Background

Dialysis is a method of achieving separation by utilizing the property that small molecular substances can pass through a semipermeable membrane in a solution, while large molecular substances cannot pass through the semipermeable membrane. At present, most of protein dialysis is to put dialysis bags into dialysis buffer solution, and when a plurality of samples are dialyzed, the dialysis bags can be intertwined with each other, so that the dialysis effect is influenced.

SUMMERY OF THE UTILITY MODEL

In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a protein purification dialysis apparatus, which can stably separate and clamp a plurality of dialysis bags, can stir a buffer solution, and ensures the effect of protein purification dialysis.

The technical scheme adopted by the utility model is as follows: the utility model provides a protein purification dialysis unit, includes barrel, upper cover and presss from both sides tight runner assembly, the upper cover is located on the barrel, press from both sides tight runner assembly and locate on covering and locate in the barrel, press from both sides tight runner assembly and include bearing, peg graft pole, support pivot, rotating electrical machines and press from both sides tight supporting component, the diapire in the barrel is located to the bearing, the peg graft pole is located on the bearing, the upper cover upper end is located to the rotating electrical machines, the rotating electrical machines output is located to support pivot one end, it locates in the barrel to support the pivot other end to run through the upper cover, it locates on the peg graft pole to support the pivot other end cover, press from both sides tight supporting component and locate in the support pivot.

Further, the clamping and supporting component comprises a placing baffle, a fixing rod, a clamping rod, a connecting column, a limiting groove and a fastening bolt, the fixed rods are arranged on the side wall of the supporting rotating shaft and distributed in a cross shape around the direction of the fixed shaft of the supporting rotating shaft, one end of the clamping rod is hinged with one end of the fixed rod close to the supporting rotating shaft, the limit groove is arranged on one side of the clamping rod away from the supporting rotating shaft, one end of the connecting column is arranged at one end of the fixed rod, which is far away from the supporting rotating shaft, the other end of the connecting column penetrates through the limiting groove, the fastening bolt is arranged on one side of the limiting groove far away from the fixed rod and is in threaded connection with the connecting column, the dialysis bag placing device is characterized in that the placing baffle is arranged in a V shape, the placing baffle is arranged below the fixing rod and the clamping rod, the bottom of the dialysis bag is placed on the placing baffle, the upper end of the dialysis bag is clamped between the fixing rod and the clamping rod, and a plurality of dialysis bags are stably clamped and placed.

Further, the upper cover is provided with a sealing ring, when the support rotating shaft is sleeved with the outside of the insertion rod, the insertion rod moves downwards to be inserted into the support rotating shaft, the upper cover is closed at the opening at the upper end of the barrel body, and the sealing ring plays a role in sealing and stabilizing the connection between the upper cover and the barrel body.

Furthermore, the upper cover is provided with a handle, so that the device is convenient to move.

Furthermore, supporting legs are arranged at the bottom of the barrel body, and the supporting legs are symmetrical in pairs, so that the supporting effect on the whole device is achieved.

Further, the bottom of the placing baffle is provided with a through groove.

After adopting the structure, the utility model has the following beneficial effects: according to the protein purification dialysis device, the plurality of dialysis bags can be stably clamped and placed through the clamping and supporting assembly, so that mutual influence caused by winding is avoided; through the tight runner assembly that presss from both sides that sets up, can rotate in the barrel and stir solution, guarantee the effect of protein purification dialysis.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model.

FIG. 1 is a schematic view of the overall structure of a protein purification dialysis apparatus according to the present invention;

FIG. 2 is a front view of a protein purification dialysis apparatus according to the present invention;

FIG. 3 is a schematic structural diagram of a clamping and rotating assembly of the protein purification dialysis apparatus according to the present invention;

fig. 4 is an enlarged view of a portion a of fig. 3.

In the drawings: 1. barrel, 2, upper cover, 3, press from both sides tight runner assembly, 4, bearing, 5, peg graft pole, 6, support pivot, 7, rotating electrical machines, 8, press from both sides tight supporting component, 9, place the baffle, 10, dead lever, 11, clamping rod, 12, spliced pole, 13, spacing groove, 14, fastening bolt, 15, sealing washer, 16, handle, 17, supporting legs, 18, logical groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

As shown in fig. 1-4, a protein purification dialysis device comprises a cylinder 1, an upper cover 2 and a clamping rotating assembly 3, wherein the upper cover 2 is arranged on the cylinder 1, and the clamping rotating assembly 3 is arranged on the upper cover 2 and is arranged in the cylinder 1.

Preferably, the upper cover 2 is provided with a sealing ring 15, when the support rotating shaft 6 is sleeved with the insertion rod 5, the insertion rod 5 moves downwards to be inserted into the support rotating shaft 6, the upper cover 2 is closed at the opening at the upper end of the cylinder body 1, and the sealing ring 15 has the effects of sealing and stabilizing the connection between the upper cover 2 and the cylinder body 1.

Preferably, the upper cover 2 is provided with a handle 16 to facilitate movement of the device.

Preferably, the bottom of the cylinder body 1 is provided with supporting legs 17, and the supporting legs 17 are symmetrical in pairs, so that the whole device is supported.

As shown in fig. 3 and 4, the clamping rotating assembly 3 includes a bearing 4, an inserting rod 5, a supporting rotating shaft 6, a rotating electrical machine 7 and a clamping supporting assembly 8, the bearing 4 is disposed on the inner bottom wall of the barrel 1, the inserting rod 5 is disposed on the bearing 4, the rotating electrical machine 7 is disposed at the upper end of the upper cover 2, one end of the supporting rotating shaft 6 is disposed at the output end of the rotating electrical machine 7, the other end of the supporting rotating shaft 6 penetrates through the upper cover 2 and is disposed in the barrel 1, the other end of the supporting rotating shaft 6 is sleeved on the inserting rod 5, and the clamping supporting assembly 8 is disposed on the supporting rotating shaft 6.

As shown in fig. 3 and 4, the clamping and supporting assembly 8 includes a placing baffle 9, a fixing rod 10, a clamping rod 11, a connecting column 12, a limiting groove 13 and a fastening bolt 14, the fixing rod 10 is disposed on the side wall of the supporting spindle 6, the fixing rod 10 is distributed in a cross shape around the fixing axis of the supporting spindle 6, one end of the clamping rod 11 is hinged to one end of the fixing rod 10 close to the supporting spindle 6, the limiting groove 13 is disposed on one side of the clamping rod 11 away from the supporting spindle 6, one end of the connecting column 12 is disposed on one end of the fixing rod 10 away from the supporting spindle 6, the other end of the connecting column 12 penetrates through the limiting groove 13, the fastening bolt 14 is disposed on one side of the limiting groove 13 away from the fixing rod 10, the fastening bolt 14 is in threaded connection with the connecting column 12, the placing baffle 9 is arranged in a V shape, the placing baffle 9 is disposed below the fixing rod 10 and the clamping rod 11, and the bottom of the dialysis bag is placed on the placing baffle 9, the upper end is clamped between the fixing rod 10 and the clamping rod 11, and a plurality of dialysis bags are stably clamped and placed.

Preferably, the bottom of the placing baffle 9 is provided with a through groove 18.

When the dialysis bag fixing device is used specifically, the supporting rotating shaft 6 is separated from the inserting rod 5, the bottom of a dialysis bag is placed on the placing baffle 9, the upper end of the dialysis bag penetrates through the position between the fixing rod 10 and the clamping rod 11, the fixing rod 10 and the clamping rod 11 are close to clamp a dialysis bag body, the fastening bolt 14 is rotated, the dialysis bag body at the upper end of the dialysis bag is clamped between the fixing rod 10 and the clamping rod 11, and a plurality of dialysis bags are stably clamped and clamped in a separated mode; pouring the solution into the cylinder 1; sleeving the bottom of the supporting rotating shaft 6 outside the inserting rod 5, and enabling the inserting rod 5 to extend into the supporting rotating shaft 6 to limit the supporting rotating shaft 6; the rotating motor 7 is started, the output end of the rotating motor 7 rotates to drive the supporting rotating shaft 6 to rotate, the supporting rotating shaft 6 rotates to drive the dialysis bag and support the whole part on the rotating shaft 6 to rotate, the solution in the cylinder body 1 is stirred, and the protein in the dialysis bag can be conveniently purified and separated out.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the utility model as defined by the appended claims.

Claims

1. A protein purification dialysis device, its characterized in that: the clamping rotating assembly is arranged on the upper cover and is arranged in the upper cover and the barrel body, the clamping rotating assembly comprises a bearing, an inserting rod, a supporting rotating shaft, a rotating motor and a clamping supporting assembly, the bearing is arranged on the inner bottom wall of the barrel body, the inserting rod is arranged on the bearing, the rotating motor is arranged at the upper end of the upper cover, one end of the supporting rotating shaft is arranged at the output end of the rotating motor, the other end of the supporting rotating shaft penetrates through the upper cover and is arranged in the barrel body, the other end of the supporting rotating shaft is sleeved on the inserting rod, and the clamping supporting assembly is arranged on the supporting rotating shaft.

2. The protein purification dialysis apparatus according to claim 1, wherein: the clamping support component comprises a placing baffle, a fixing rod, a clamping rod, a connecting column, a limiting groove and a fastening bolt, the fixing rod is arranged on the side wall of the supporting rotary shaft, the fixing rod is distributed in a mode of being crossed around the direction of the supporting rotary shaft fixing shaft, one end of the clamping rod is hinged to the fixing rod and close to one end of the supporting rotary shaft, the limiting groove is arranged on the clamping rod and is far away from one side of the supporting rotary shaft, one end of the connecting column is arranged on the fixing rod and is far away from one end of the supporting rotary shaft, the other end of the connecting column penetrates through the limiting groove, the fastening bolt is arranged on the limiting groove and is far away from one side of the fixing rod, the fastening bolt is in threaded connection with the connecting column, the placing baffle is arranged in a V shape, and the placing baffle is arranged below the fixing rod and the clamping rod.

3. A protein purification dialysis apparatus according to claim 2, wherein: the upper cover is provided with a sealing ring.

4. A protein purification dialysis apparatus according to claim 3, wherein: the upper cover is provided with a handle.

5. A protein purification dialysis device according to claim 4, wherein: the bottom of the barrel is provided with supporting legs, and the supporting legs are symmetrical pairwise.

6. A protein purification dialysis device according to claim 5, wherein: the bottom of the placing baffle is provided with a through groove.