CN216274186U

CN216274186U - Cell culture device convenient to add culture solution

Info

Publication number: CN216274186U
Application number: CN202122593653.3U
Authority: CN
Inventors: 钟雷鸣
Original assignee: Tianjin Saieryuan Biotechnology Co ltd
Current assignee: Tianjin Saieryuan Biotechnology Co ltd
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2022-04-12
Anticipated expiration: 2031-10-27

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture device convenient for adding culture solution, which comprises a substrate, wherein a culture solution box is fixed at the back of the top end of the substrate, a sliding groove is formed in the surface of the culture solution box, a positioning block is arranged in the sliding groove, a plate shell is fixed on the front side of the positioning block, a liquid pump and quantitative tubes are arranged on the left side of the bottom end of the plate shell, a liquid inlet end of the liquid pump is provided with a liquid suction pipe, a liquid outlet end of the liquid pump is provided with liquid outlet tubes, four groups of quantitative tubes are arranged, the four groups of quantitative tubes are communicated through horizontal through tubes, the bottom end inside the quantitative tube is provided with an electromagnetic valve, and the top of each quantitative tube penetrates through the inside of the plate shell. The utility model provides a cell culture device convenient to add culture solution, is provided with the ration pipe through the bottom of board shell, and the ration pipe will form accurate ration to the culture solution and add, can form the interpolation to the multiunit culture dish simultaneously, improves the convenient of culture solution interpolation in-process greatly and the accuracy of interpolation.

Description

Cell culture device convenient to add culture solution

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture device convenient for adding culture solution.

Background

Currently, cell culture techniques refer to the growth of cells under in vitro conditions, during which the cells no longer form tissues (animals).

At present at cell culture's in-process, need regularly add to the culture solution of cell, and the mode of traditional interpolation is by artifical manual interpolation, and manual interpolation needs staff record to add the cycle, if forget to add will lead to cell death or growth slowly, manual interpolation can't guarantee also that the cell needs the accuracy of nutrient solution simultaneously, will lead to the inconvenience in the cell culture greatly, in order to solve the problem that exists among the above-mentioned, consequently, we propose a cell culture device of being convenient for add the culture solution.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to a cell culture apparatus that facilitates addition of a culture solution to solve the problems of the background art described above.

For realizing above-mentioned purpose, a cell culture device convenient to add culture solution, including the base plate, the back on base plate top is fixed with the culture solution case, the spout has been seted up on the surface of culture solution case, and the spout embeds there is the locating piece, the front side of locating piece is fixed with the shell, the left side of shell bottom is provided with liquid pump and dosing pipe, the feed liquor end of liquid pump is provided with the pipette, the play liquid end of liquid pump is provided with the drain pipe, dosing pipe is provided with four groups, four groups dosing pipe is linked together through horizontal siphunculus, the inside bottom of dosing pipe is provided with the solenoid valve, the inside in the shell is run through at dosing pipe's top.

Preferably, the left side intercommunication in culture solution case top has annotates the liquid pipe, the front side in culture solution case top is provided with the motor, the motor shaft of motor runs through in the inside top of spout, be connected with the lead screw on the motor shaft of motor.

Preferably, the right side at culture solution case top is provided with the switch, the switch constitutes electric connection with motor, solenoid valve and liquid pump.

Preferably, the screw rod is located inside the thread groove, the thread groove is formed inside the positioning block, and the positioning block is matched with the sliding groove in size and forms a sliding mechanism.

Preferably, the screw rod is matched with the thread size of the thread groove and forms a rotating mechanism.

Preferably, the front side of the top end of the substrate is provided with four groups of limiting grooves, and the four groups of limiting grooves are located under the four groups of quantitative tubes.

Preferably, the tail end of the pipette is communicated with the bottom end of the left side of the culture solution box, and the tail end of the liquid outlet pipe is communicated with the top end of the left side of the quantifying pipe.

Preferably, the left side of culture solution case is provided with the observation strip, and observes the strip and be the glass material.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the culture medium quantitative feeding device, the quantitative tube is arranged at the bottom of the plate shell, the culture medium is accurately and quantitatively fed by the quantitative tube, and meanwhile, multiple groups of culture dishes can be fed, so that the convenience and the accuracy of feeding in the culture medium feeding process are greatly improved.

2. According to the utility model, the plate shell is arranged on the lifting mechanism, and in the process of lifting and feeding the quantitative tube, the quantitative tube can change the distance between the quantitative tube and the culture dish, so that the problems that the quantitative tube is too high and splashes when the culture solution is injected are avoided, and the accuracy of the culture solution feeding is not influenced.

3. According to the culture dish fixing device, the limiting groove is formed in the top of the base plate, and is beneficial to limiting and placing the culture dish, so that the culture dish is accurately placed at the bottom of the quantifying tube, and the convenient effect of adding a culture solution is improved.

Drawings

FIG. 1 is a schematic view showing the overall structure of a cell culture apparatus for facilitating the addition of a culture solution according to the present invention;

FIG. 2 is a schematic side view of a substrate of a cell culture apparatus for facilitating the addition of a culture medium according to the present invention;

FIG. 3 is a schematic diagram of the internal structure of a shell of a cell culture apparatus for facilitating the addition of a culture medium according to the present invention.

In the figure: 1. a substrate; 2. a culture solution box; 3. a motor; 4. a chute; 5. positioning blocks; 6. a thread groove; 7. a screw rod; 8. a plate shell; 9. a liquid pump; 10. a liquid injection pipe; 11. a dosing tube; 12. a pipette; 13. an electromagnetic valve; 14. a limiting groove; 15. a liquid outlet pipe; 16. horizontal through pipes; 17. an observation strip; 18. and (4) switching.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example (b):

referring to fig. 1-3, which are a preferred embodiment of the present invention, a cell culture apparatus convenient for adding a culture solution includes a substrate 1, a culture solution tank 2 is fixed on a back of a top end of the substrate 1, a chute 4 is formed on a surface of the culture solution tank 2, a positioning block 5 is disposed in the chute 4, a plate housing 8 is fixed on a front side of the positioning block 5, a liquid pump 9 and a quantitative tube 11 are disposed on a left side of a bottom end of the plate housing 8, a liquid inlet end of the liquid pump 9 is provided with a liquid suction pipe 12, a liquid outlet end of the liquid pump 9 is provided with a liquid outlet pipe 15, the quantitative tubes 11 are provided with four groups, the four groups of quantitative tubes 11 are communicated with each other through a horizontal through pipe 16, an electromagnetic valve 13 is disposed at a bottom end inside the quantitative tube 11, a top of the quantitative tube 11 penetrates through the inside of the plate housing 8, a quantitative tube 11 is disposed at a bottom of the plate housing 8, and the quantitative tube 11 is used for accurately and quantitatively adding the culture solution, meanwhile, the culture dishes can be added, so that convenience and accuracy in the culture solution adding process are greatly improved.

Specifically, the left side intercommunication on 2 tops of culture solution case has notes liquid pipe 10, the front side on 2 tops of culture solution case is provided with motor 3, the motor shaft of motor 3 runs through in the inside top of spout 4, be connected with lead screw 7 on the motor shaft of motor 3.

Specifically, a switch 18 is arranged on the right side of the top end of the culture solution box 2, and the switch 18 is electrically connected with the motor 3, the electromagnetic valve 13 and the liquid pump 9.

Specifically, the screw rod 7 is located inside the thread groove 6, the thread groove 6 is arranged inside the positioning block 5, and the positioning block 5 is matched with the sliding groove 4 in size and forms a sliding mechanism. The positioning block 5 is matched with the sliding groove 4 in size, so that the stability of the plate shell 8 in the lifting process can be ensured.

Specifically, the screw rod 7 is matched with the thread size of the thread groove 6, and forms a rotating mechanism. The screw rod 7 is located in the inner rotating process of the thread groove 6, and the positioning block 5 is lifted based on the inner part of the sliding groove 4.

Specifically, the front side of the top end of the substrate 1 is provided with four sets of limiting grooves 14, and the four sets of limiting grooves 14 are located under the four sets of dosing tubes 11. Facilitating rapid placement of the culture dish at the bottom of the dosing tube 11.

Specifically, the end of the pipette 12 is communicated with the bottom end of the left side of the culture solution box 2, and the end of the drain pipe 15 is communicated with the top end of the left side of the quantifying pipe 11.

Specifically, the left side of culture solution box 2 is provided with observation strip 17, and observation strip 17 is the glass material. Is favorable for observing the culture solution storage in the culture solution box 2.

In this embodiment, the liquid injection tube 10 injects the culture solution into the culture solution tank 2 for storage, when the culture solution needs to be added, the culture dish is first placed into the limiting groove 14 formed on the top of the substrate 1 to form limiting, the switch 18 starts the liquid pump 9 to work, the liquid pump 9 sucks the culture solution in the culture solution tank 2 into the liquid outlet tube 15 through the liquid suction tube 12, the liquid outlet tube 15 drives the culture solution to enter the quantitative tube 11, meanwhile, the four quantitative tubes 11 carry out culture solution transportation through the horizontal through tube 16, finally, the quantitative tube 11 is filled with the culture solution, after the liquid pump 9 is closed, the switch 18 starts the motor 3, the motor 3 drives the screw rod 7 to rotate in the thread groove 6 formed in the positioning block 5, at this time, the positioning block 5 descends based on the sliding groove 4 to make the bottom of the quantitative tube 11 approach the culture dish, finally, the switch 18 starts the electromagnetic valve 13, the culture solution accurately confirmed in the quantitative tube 11 directly enters the culture dish, greatly improving the accuracy of the culture solution in the adding process.

While the utility model has been described in further detail in connection with specific embodiments thereof, it will be understood that the utility model is not limited thereto, and that various other modifications and substitutions may be made by those skilled in the art without departing from the spirit of the utility model, which should be considered to be within the scope of the utility model as defined by the appended claims.

Claims

1. The utility model provides a cell culture device convenient to add culture solution which characterized in that: including base plate (1), the back on base plate (1) top is fixed with culture solution box (2), spout (4) have been seted up on the surface of culture solution box (2), and spout (4) embeds there is locating piece (5), the front side of locating piece (5) is fixed with board shell (8), the left side of board shell (8) bottom is provided with liquid pump (9) and buret (11), the feed liquor end of liquid pump (9) is provided with pipette (12), the play liquid end of liquid pump (9) is provided with drain pipe (15), buret (11) are provided with four groups, four groups buret (11) are linked together through horizontal siphunculus (16), the inside bottom of buret (11) is provided with solenoid valve (13), the inside in board shell (8) is run through at the top of buret (11).

2. A cell culture apparatus for facilitating the addition of culture fluid according to claim 1, wherein: the left side intercommunication on culture solution case (2) top has notes liquid pipe (10), the front side on culture solution case (2) top is provided with motor (3), the motor shaft of motor (3) runs through in the inside top of spout (4), be connected with lead screw (7) on the motor shaft of motor (3).

3. A cell culture apparatus for facilitating the addition of culture fluid according to claim 2, wherein: the right side on culture solution case (2) top is provided with switch (18), switch (18) and motor (3), solenoid valve (13) and liquid pump (9) constitute electric connection.

4. A cell culture apparatus for facilitating the addition of culture fluid according to claim 2, wherein: the screw rod (7) is located inside the thread groove (6), the thread groove (6) is arranged inside the positioning block (5), and the positioning block (5) is matched with the sliding groove (4) in size and forms a sliding mechanism.

5. A cell culture apparatus for facilitating the addition of culture fluid according to claim 4, wherein: the screw rod (7) is matched with the thread size of the thread groove (6) and forms a rotating mechanism.

6. A cell culture apparatus for facilitating the addition of culture fluid according to claim 1, wherein: limiting grooves (14) are formed in the front side of the top end of the base plate (1), four groups of limiting grooves (14) are formed in the limiting grooves (14), and the four groups of limiting grooves (14) are located under the four groups of quantitative tubes (11).

7. A cell culture apparatus for facilitating the addition of culture fluid according to claim 1, wherein: the tail end of the pipette (12) is communicated with the bottom end of the left side of the culture solution box (2), and the tail end of the liquid outlet pipe (15) is communicated with the top end of the left side of the quantifying pipe (11).

8. A cell culture apparatus for facilitating the addition of culture fluid according to claim 1, wherein: the left side of culture solution case (2) is provided with observation strip (17), and observation strip (17) are the glass material.