CN216247780U - Single cell mass spectrum detection device - Google Patents
Single cell mass spectrum detection device Download PDFInfo
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- CN216247780U CN216247780U CN202122851869.5U CN202122851869U CN216247780U CN 216247780 U CN216247780 U CN 216247780U CN 202122851869 U CN202122851869 U CN 202122851869U CN 216247780 U CN216247780 U CN 216247780U
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Abstract
The utility model discloses a single-cell mass spectrum detection device, belonging to the technical field of cell mass spectrum detection, and comprising a support component, wherein an extraction system is fixedly arranged on the support component, a nano probe is arranged on the support component, a glass plate is arranged below the nano probe, a sample injector is arranged on the support component, and a driving device is arranged on the support component; an observation mechanism is arranged below the driving device, and the utility model has the beneficial effects that: through setting up drive arrangement, can realize the nanoprobe in the extraction system from top to bottom, around and control three-dimensional motion to can make the nanoprobe aim at unicellular extraction position, thereby accomplish the sample automatically, and the sampling position is accurate, accelerate extraction rate, through setting up observation mechanism, shoot nanoprobe and unicellular during the extraction, thereby can aim at unicellular, realize accurate sample.
Description
Technical Field
The utility model relates to the technical field of cell mass spectrometry detection, in particular to a single-cell mass spectrometry detection device.
Background
The single cell mass spectrum detection technology is beneficial to improving medical progress, and a biological mass spectrometer is used for detection and analysis; in mass spectrometry, a single-cell organism needs to be sampled.
When current unicellular mass spectrometry detected, at present by artifical sampling to the unicellular, sampling speed was slow to the sample position is accurate enough, can get the thing that does not need in the unicellular, thereby is unfavorable for the detection.
SUMMERY OF THE UTILITY MODEL
The utility model is provided in view of the problems existing in the existing single-cell mass spectrum detection device.
Therefore, the utility model aims to provide a single cell mass spectrum detection device, which drives a nano probe in a sampling system to move through a driving device, and accurately aligns the sampling position of a single cell through an observation device, so that sampling is quickly completed, accurate sampling is realized, and the problems that when the existing single cell mass spectrum detection is carried out, the single cell is sampled manually, the sampling speed is low, the sampling position is not accurate enough, and unnecessary objects in the single cell can be taken, so that the detection is not facilitated are solved.
To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions:
the single cell mass spectrum detection device comprises a supporting component, wherein an extraction system is fixedly arranged on the supporting component, a nano probe is arranged on the supporting component, a glass plate is arranged below the nano probe, a sample injector is arranged on the supporting component, and a driving device is arranged on the supporting component; an observation mechanism is arranged below the driving device;
the driving device comprises a servo motor, an output shaft of the servo motor is fixedly connected with a first lead screw, the outer wall of the first lead screw is sleeved with a first belt pulley, the outer wall of the first lead screw is in threaded connection with a moving seat, the inner wall of the moving seat is in threaded connection with a second lead screw, the outer wall of the second lead screw is sleeved with a second belt pulley, the first belt pulley and the second belt pulley are connected through belt transmission, the bottom end of the moving seat is fixedly connected with a supporting seat, the bottom end of the supporting seat is provided with a first chute and a second chute, the inner wall of the first chute is in sliding connection with a first slide block, the outer wall of the first slide block is fixedly connected with a first electric telescopic rod, one end of the first electric telescopic rod is fixedly connected with the inner wall of the first chute, the bottom end of the first slide block is fixedly connected with a supporting rod, the inner wall of the second chute is in sliding connection with a second slide block, and the outer wall of the second slide block is fixedly provided with a second electric telescopic rod, one end of the second electric telescopic rod is fixedly connected with the inner wall of the second sliding groove, the bottom end of the second sliding block is connected with the mounting block in a rotating mode, the mounting block is connected with the third electric telescopic rod in a rotating mode through a pin shaft, the bottom end of the mounting block is fixedly connected with the fourth electric telescopic rod, and the bottom end of the fourth electric telescopic rod is connected with the nanoprobe.
As a preferable embodiment of the single-cell mass spectrometry detection apparatus of the present invention, wherein: the supporting assembly comprises a bottom plate, a supporting column is fixedly installed at the top end of the bottom plate, and a top plate is fixedly installed at the top end of the supporting column.
As a preferable embodiment of the single-cell mass spectrometry detection apparatus of the present invention, wherein: the movable seat is provided with a through hole, the inner wall of the through hole is connected with the stabilizing rod in a sliding mode, and two ends of the stabilizing rod are fixedly connected with the supporting columns.
As a preferable embodiment of the single-cell mass spectrometry detection apparatus of the present invention, wherein: one end of the third electric telescopic rod, which is far away from the mounting block, is movably connected with the supporting rod.
As a preferable embodiment of the single-cell mass spectrometry detection apparatus of the present invention, wherein: the sample injector is installed on the support column, and the extraction system is installed on the support column.
As a preferable embodiment of the single-cell mass spectrometry detection apparatus of the present invention, wherein: the observation mechanism comprises a microscope, the microscope is installed on a supporting plate, third sliders are fixedly installed at two ends of the supporting plate and slidably connected with a slide seat, a first lantern ring is sleeved on the outer wall of an eyepiece of the microscope, a connecting rod is fixedly installed on the outer wall of the first lantern ring and is far away from one end of the first lantern ring, a fixing rod is sleeved on the inner wall of the second lantern ring, and a camera is installed on the fixing rod.
As a preferable embodiment of the single-cell mass spectrometry detection apparatus of the present invention, wherein: the slide is installed on the support column, the third spout has been seted up on the slide, third spout inner wall sliding connection third slider.
As a preferable embodiment of the single-cell mass spectrometry detection apparatus of the present invention, wherein: the first screw rod and the second screw rod are rotatably connected with the supporting column through bearings, the glass plate is installed on the supporting column, and the glass plate is located above the microscope.
Compared with the prior art:
1. by arranging the driving device, the nano probe in the extraction system can move up and down, back and forth and left and right in three dimensions, so that the nano probe can be aligned to the single cell extraction position, the sampling can be automatically completed, the sampling position is accurate, and the extraction speed is accelerated;
2. through setting up observation mechanism, shoot nanometer probe and unicellular during the extraction to can aim at the unicellular, realize accurate sample.
Drawings
FIG. 1 is a schematic structural view provided by the present invention;
FIG. 2 is a partial top view of the drive of FIG. 1 in accordance with the present invention;
FIG. 3 is a cross-sectional view of a support base according to the present invention;
FIG. 4 is an enlarged view taken at A of FIG. 1 according to the present invention;
fig. 5 is a perspective view of a first collar provided by the present invention.
In the figure: the device comprises a driving device 1, a second slider 10, a second electric telescopic rod 101, a first electric telescopic rod 102, a stabilizing rod 103, a fourth electric telescopic rod 104, a servo motor 11, a first screw rod 12, a moving seat 13, a through hole 131, a second belt pulley 14, a second screw rod 15, a first belt pulley 16, a supporting seat 17, a supporting rod 171, a first slider 1711, a first chute 172, a second chute 173, a third electric telescopic rod 18, a mounting block 19, an extraction system 2, a nanoprobe 21, a supporting component 3, a supporting column 31, a bottom plate 32, a top plate 33, an observation mechanism 4, a microscope 41, a sliding seat 42, a third chute 421, a third slider 43, a supporting plate 44, a fixing rod 45, a first collar 46, a connecting rod 47, a second collar 48, a camera 49, a sample injector 5 and a glass plate 6.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail with reference to the accompanying drawings.
The utility model provides a single cell mass spectrometry detection device, please refer to fig. 1-5, which comprises a support component 3, wherein an extraction system 2 is fixedly arranged on the support component 3, a nano probe 21 is arranged on the support component 3, a glass plate 6 is arranged below the nano probe 21, a sample injector 5 is arranged on the support component 3, and a driving device 1 is arranged on the support component 3; the installation and extraction system 2 is connected with a terminal computer; an observation mechanism 4 is arranged below the driving device 1; the extraction system 2 is set to FIS300 and the injector 5 is set to YTQ 300;
the driving device 1 comprises a servo motor 11, an output shaft of the servo motor 11 is fixedly connected with a first lead screw 12, the outer wall of the first lead screw 12 is sleeved with a first belt pulley 16, the outer wall of the first lead screw 12 is in threaded connection with a moving seat 13, the inner wall of the moving seat 13 is in threaded connection with a second lead screw 15, the outer wall of the second lead screw 15 is sleeved with a second belt pulley 14, the first belt pulley 16 is in transmission connection with the second belt pulley 14 through a belt, the bottom end of the moving seat 13 is fixedly connected with a supporting seat 17, the bottom end of the supporting seat 17 is provided with a first sliding groove 172 and a second sliding groove 173, the inner wall of the first sliding groove 172 is in sliding connection with a first sliding block 1711, the outer wall of the first sliding block 1711 is fixedly connected with a first electric telescopic rod 102, one end of the first electric telescopic rod 102 is fixedly connected with the inner wall of the first sliding groove 172, the bottom end of the first sliding block 171171, the inner wall of the second sliding groove 173 is in sliding connection with a second sliding block 10, a second electric telescopic rod 101 is fixedly installed on the outer wall of the second sliding block 10, one end of the second electric telescopic rod 101 is fixedly connected with the inner wall of the second sliding groove 173, the bottom end of the second sliding block 10 is rotatably connected with an installation block 19, the installation block 19 is rotatably connected with a third electric telescopic rod 18 through a pin shaft, the bottom end of the installation block 19 is fixedly connected with a fourth electric telescopic rod 104, and the bottom end of the fourth electric telescopic rod 104 is connected with a nanometer probe 21;
further, the support assembly 3 comprises a bottom plate 32, a support column 31 is fixedly mounted at the top end of the bottom plate 32, and a top plate 33 is fixedly mounted at the top end of the support column 31; the support column 31 functions to support and fix the driving device 1.
Further, a through hole 131 is formed in the moving seat 13, the inner wall of the through hole 131 is slidably connected with the stabilizing rod 103, and two ends of the stabilizing rod 103 are fixedly connected with the supporting columns 31; the stabilizing rod 103 is used for maintaining the stable movement of the movable base 13 and supporting the movable base 13, thereby reducing the resistance between the first lead screw 12, the second lead screw 15 and the movable base 13.
Further, one end of the third electric telescopic rod 18, which is far away from the mounting block 19, is movably connected with a support rod 171; the third electric telescopic rod 18 is used for driving the mounting block 19 to rotate, so as to send the extracted sample to the sample injector 5.
Further, the sample injector 5 is mounted on a support column 31, and the extraction system 2 is mounted on the support column 31; the sample injector 5 serves to store the sampled sample.
Further, the observation mechanism 4 includes a microscope 41, the microscope 41 is mounted on a support plate 44, third sliders 43 are fixedly mounted at two ends of the support plate 44, the third sliders 43 are slidably connected to the slide seat 42, an outer wall of an eyepiece of the microscope 41 is sleeved with a first collar 46, a connecting rod 47 is fixedly mounted on an outer wall of the first collar 46, one end of the connecting rod 47, which is far away from the first collar 46, is fixedly connected to a second collar 48, an inner wall of the second collar 48 is sleeved with a fixing rod 45, and a camera 49 is mounted on the fixing rod 45; the microscope 41 is set as AP-SMALDI 10, and the camera 49 is used for observing single cells; the camera 49 communicates with a computer; the slide 42 serves to fix the microscope 41 and to effect the raising and lowering of the microscope 41, changing the distance to the glass plate 6.
Further, the slide seat 42 is installed on the support column 31, a third slide groove 421 is formed in the slide seat 42, and an inner wall of the third slide groove 421 is slidably connected to the third slide block 43.
Further, the first screw rod 12 and the second screw rod 15 are rotatably connected with a support column 31 through bearings, the glass plate 6 is mounted on the support column 31, and the glass plate 6 is located above the microscope 41; the action of the first screw 12 and the second screw 15 drives the movable base 13 to move.
When the device is used specifically, a single cell is placed on the glass plate 6, the camera 49 shoots the single cell, when the nanoprobe 21 is not positioned right above the single cell, the servo motor 11 is started, the output shaft of the servo motor 11 drives the first screw rod 12 to rotate, the first screw rod 12 drives the second screw rod 15 to rotate together through the transmission of the first belt pulley 16 and the second belt pulley 14, and the first screw rod 12 and the second screw rod 15 drive the moving seat 13 to move; the second electric telescopic rod 101 and the first electric telescopic rod 102 stretch to drive the nanoprobe 21 to move until the nanoprobe 21 is positioned right above the single cell; the output of the fourth electric telescopic rod 104 drives the nanoprobe 21 to descend, the extraction system 2 samples through the nanoprobe 21, and after the sampling is completed, the third electric telescopic rod 18 extends to drive the installation block 19 to rotate, so that the nanoprobe 21 is aligned with the sample injector 5, and the sampling is completed.
While the utility model has been described above with reference to an embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the utility model. In particular, the various features of the disclosed embodiments of the utility model may be used in any combination, provided that no structural conflict exists, and the combinations are not exhaustively described in this specification merely for the sake of brevity and resource conservation. Therefore, it is intended that the utility model not be limited to the particular embodiments disclosed, but that the utility model will include all embodiments falling within the scope of the appended claims.
Claims (8)
1. Unicellular mass spectrometry detection device, including supporting component (3), fixed mounting extraction system (2) on supporting component (3), be equipped with nanometer probe (21) on supporting component (3), nanometer probe (21) below is equipped with glass board (6), its characterized in that: a sample injector (5) is arranged on the supporting component (3), and a driving device (1) is arranged on the supporting component (3); an observation mechanism (4) is arranged below the driving device (1);
the driving device (1) comprises a servo motor (11), an output shaft of the servo motor (11) is fixedly connected with a first lead screw (12), the outer wall of the first lead screw (12) is sleeved with a first belt pulley (16), the outer wall of the first lead screw (12) is in threaded connection with a moving seat (13), the inner wall of the moving seat (13) is in threaded connection with a second lead screw (15), the outer wall of the second lead screw (15) is sleeved with a second belt pulley (14), the first belt pulley (16) is connected with the second belt pulley (14) through belt transmission, the bottom end of the moving seat (13) is fixedly connected with a supporting seat (17), the bottom end of the supporting seat (17) is provided with a first sliding chute (172) and a second sliding chute (173), the inner wall of the first sliding chute (172) is slidably connected with a first sliding block (1711), the outer wall of the first sliding block (1711) is fixedly connected with a first electric telescopic rod (102), first electric telescopic handle (102) one end fixed connection first spout (172) inner wall, first slider (1711) bottom fixed connection bracing piece (171), second spout (173) inner wall sliding connection second slider (10), second slider (10) outer wall fixed mounting second electric telescopic handle (101), second electric telescopic handle (101) one end fixed connection second spout (173) inner wall, second slider (10) bottom is rotated and is connected installation piece (19), installation piece (19) are rotated through the round pin axle and are connected third electric telescopic handle (18), installation piece (19) bottom fixed connection fourth electric telescopic handle (104), nanoprobe (21) is connected to fourth electric telescopic handle (104) bottom.
2. The single-cell mass spectrometry detection device according to claim 1, wherein the support assembly (3) comprises a bottom plate (32), a support column (31) is fixedly mounted at the top end of the bottom plate (32), and a top plate (33) is fixedly mounted at the top end of the support column (31).
3. The single-cell mass spectrometry detection device according to claim 1, wherein the moving seat (13) is provided with a through hole (131), the inner wall of the through hole (131) is slidably connected with the stabilizing rod (103), and two ends of the stabilizing rod (103) are fixedly connected with the supporting columns (31).
4. The single-cell mass spectrometry detection device of claim 1, wherein one end of the third electric telescopic rod (18) far away from the mounting block (19) is movably connected with a support rod (171).
5. The single-cell mass spectrometry detection apparatus according to claim 1, wherein the injector (5) is mounted on a support column (31), and the extraction system (2) is mounted on the support column (31).
6. The single cell mass spectrometry detection device according to claim 1, wherein the observation mechanism (4) comprises a microscope (41), the microscope (41) is mounted on a support plate (44), third sliders (43) are fixedly mounted at two ends of the support plate (44), the third sliders (43) are slidably connected with a slide seat (42), an outer wall of an eyepiece of the microscope (41) is sleeved with a first sleeve ring (46), a connecting rod (47) is fixedly mounted on an outer wall of the first sleeve ring (46), one end, far away from the first sleeve ring (46), of the connecting rod (47) is fixedly connected with a second sleeve ring (48), a fixing rod (45) is sleeved on an inner wall of the second sleeve ring (48), and a camera (49) is mounted on the fixing rod (45).
7. The single-cell mass spectrometry detection device according to claim 6, wherein the sliding base (42) is installed on the supporting column (31), a third sliding groove (421) is formed on the sliding base (42), and an inner wall of the third sliding groove (421) is slidably connected with the third sliding block (43).
8. The single-cell mass spectrometry detection device according to claim 1, wherein the first lead screw (12) and the second lead screw (15) are rotatably connected with a support column (31) through bearings, the glass plate (6) is mounted on the support column (31), and the glass plate (6) is positioned above the microscope (41).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122851869.5U CN216247780U (en) | 2021-11-20 | 2021-11-20 | Single cell mass spectrum detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122851869.5U CN216247780U (en) | 2021-11-20 | 2021-11-20 | Single cell mass spectrum detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216247780U true CN216247780U (en) | 2022-04-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122851869.5U Active CN216247780U (en) | 2021-11-20 | 2021-11-20 | Single cell mass spectrum detection device |
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|---|---|
| CN (1) | CN216247780U (en) |
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2021
- 2021-11-20 CN CN202122851869.5U patent/CN216247780U/en active Active
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