CN111534424A - Detection device for fish kidney tissue cells - Google Patents

Detection device for fish kidney tissue cells Download PDF

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Publication number
CN111534424A
CN111534424A CN202010414416.3A CN202010414416A CN111534424A CN 111534424 A CN111534424 A CN 111534424A CN 202010414416 A CN202010414416 A CN 202010414416A CN 111534424 A CN111534424 A CN 111534424A
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CN
China
Prior art keywords
wall
ring
base
detection device
extension
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Pending
Application number
CN202010414416.3A
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Chinese (zh)
Inventor
李天云
裴得胜
贾盼盼
李勇志
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Chongqing University
Chongqing Institute of Green and Intelligent Technology of CAS
Original Assignee
Chongqing University
Chongqing Institute of Green and Intelligent Technology of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Chongqing University, Chongqing Institute of Green and Intelligent Technology of CAS filed Critical Chongqing University
Priority to CN202010414416.3A priority Critical patent/CN111534424A/en
Publication of CN111534424A publication Critical patent/CN111534424A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material

Abstract

The invention discloses a detection device for fish kidney tissue cells, which comprises a base, wherein an upright post is arranged on the left side of the outer wall of the top of the base, a microscope body is arranged at the top of the upright post, a rotating motor is arranged on the right side of the inner wall of the top of the base, a placing plate is arranged on an output shaft of the rotating motor, a first through hole is formed in the circle center of the top of the placing plate and the outer wall of the top of the base, and a first illuminating lamp is arranged on the inner wall of the bottom of the base. According to the invention, the placing plate can be driven by the rotating motor to rotate out of the bottom of the microscope body, fish kidney slice cells are placed at the first through hole on the placing plate, the placing plate is driven by the rotating motor to rotate to the bottom of the microscope body for observation after fixation is completed, and meanwhile, the first through hole is illuminated by the first illuminating lamp, so that observation and detection are facilitated, cell tissues are convenient to disassemble and assemble, the specific position of the cell slice does not need to be adjusted every time, and the device is convenient and efficient.

Description

Detection device for fish kidney tissue cells
Technical Field
The invention relates to the technical field of fish cell detection, in particular to a detection device for fish kidney tissue cells.
Background
When detecting fish kidney histiocyte, need use the microscope to observe and detect, current microscope exists the fixed inconvenient problem of dismantling of cell section.
Through retrieval, chinese patent publication No. CN205443295U discloses a cell assay detection device for kidney transplantation, which comprises a device body, wherein the device body comprises a base, a stand column, a cross beam, a clasping seat and a lens barrel, the bottom end of the stand column is fixedly connected with the base, the upper end of the stand column is movably connected with a plurality of cross beams, each cross beam is respectively and fixedly connected with the clasping seat, the clasping seat is fixedly connected with the lens barrel, the upper end of the lens barrel is fixedly connected with an eyepiece, and the lower end of the lens barrel is fixedly connected with an objective lens. The cellular assay detecting device for kidney transplantation in the above patent has the following disadvantages: when the microscope is used, the problem that the microscope body is blocked and inconvenient exists when the cell slice is detached or fixed.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a detection device for fish kidney tissue cells.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a detection apparatus for be used for fish kidney histocyte, includes the base, the left side of base top outer wall is equipped with the stand, and the top of stand is equipped with the microscope body, the right side of base top inner wall is equipped with the rotating electrical machines, and the output shaft of rotating electrical machines is equipped with places the board, the centre of a circle department of placing the board top and the top outer wall of base have all seted up first perforation, and base bottom inner wall is equipped with first light.
Preferably, the microscope body, the first illuminating lamp and the opening of the first through hole are positioned in the same vertical direction, and the fixing clip is mounted on the top of the placing plate along the opening of the first through hole.
Preferably, the bottom of base right side outer wall is pegged graft and is had two extension rods, and the one end that two extension rods extended to base right side outer wall is equipped with same fly leaf, and the one end that two extension rods extended to the base inside is equipped with same limiting plate, and one side that the fly leaf is close to the base is equipped with the magnet piece, and the first spout has been seted up at the top of fly leaf.
Preferably, the second chute has been seted up to the top outer wall of base, and place one side of board bottom and be equipped with the slider, the external diameter of slider and the internal diameter of first chute, the internal diameter looks adaptation of second chute.
Preferably, the circumference of the upright post is sleeved with a fixed ring, and the outer wall of one side of the fixed ring is provided with a movable ring.
Preferably, the circumference inner wall sliding connection of activity circle has first extension circle, and the circumference outer wall sliding connection of activity circle has the second to extend the circle, and the bottom of first extension circle circumference inner wall is equipped with the second light.
Preferably, the bottom that the circle circumference inner wall was extended to the second is equipped with evenly distributed's connection rope, and evenly distributed's second perforation is seted up at the top of activity circle circumference outer wall, connects the one end that the rope kept away from the second and extends the circle and passes the second perforation and the first top fixed connection that extends the circle.
Preferably, the second extends the circumference inner wall of circle and offers evenly distributed's second guide way, and evenly distributed's first guide way is offered to the circumference outer wall of activity circle, and the circumference inner wall bottom that the second extended the circle is provided with evenly distributed's first guide block, and the top of activity circle circumference outer wall is provided with evenly distributed's second guide block.
Preferably, the bottom of the outer wall of the right side of the first extension ring is provided with a handle, the circumference of the bottom of the movable ring is fixed with a magnet piece, and the cross section of the side edge of the first extension ring is of an L-shaped structure.
Preferably, one side of the placing plate is provided with a groove, the bottom of one side of the upright column close to the placing plate is provided with a limiting rod, and the outer diameter of the limiting rod is matched with the inner diameter of the groove.
The invention has the beneficial effects that:
1. according to the detection device for the fish kidney tissue cells, the rotary motor, the placing plate, the first through hole and the first illuminating lamp are arranged, the placing plate can be driven by the rotary motor to rotate out of the bottom of the microscope body, the fish kidney slice cells are placed at the first through hole on the placing plate, the placing plate is driven by the rotary motor to rotate to the bottom of the microscope body for observation after fixation is completed, meanwhile, the first illuminating lamp illuminates the first through hole, observation and detection are facilitated, cell tissues are convenient to disassemble and assemble, the specific position of the cell slice does not need to be adjusted every time, and the detection device is convenient and efficient.
2. According to the detection device for the fish kidney tissue cells, the movable plate and the first sliding groove are arranged, when cell slices need to be disassembled and assembled, the movable plate can be pulled, the limiting plate limits the movable plate, when the placing plate is screwed out, the sliding blocks sequentially slide in the second sliding groove and the first sliding groove, and the movable plate supports the bottom of the screwed-out placing plate.
3. According to the detection device for the fish kidney tissue cells, the connecting rope, the fixed ring, the movable ring, the first extension ring, the second extension ring and the second illuminating lamp are arranged, when the placing plate is assembled and reset to the bottom of the microscope body, the fixed ring is rotated to enable the movable ring to rotate to the top of the placing plate, the first extension ring is pulled downwards to be unfolded, the top of the first extension ring pulls the second extension ring through the connecting rope while moving downwards, the second extension ring is enabled to be unfolded upwards, the second illuminating lamp illuminates the placing plate during detection and observation, meanwhile, the second extension ring and the movable ring shield surrounding interference light rays, and the detection device is practical, flexible and convenient, and ensures detection accuracy.
4. According to the detection device for the fish kidney tissue cells, the magnet pieces are arranged, the first extension ring is stirred upwards after detection is finished, the bottom of the movable ring is in contact with the bottom of the first extension ring and is adsorbed and fixed through the magnet pieces, and the second extension ring moves downwards due to gravity and is reset to the periphery of the movable ring, so that the detection device is practical and convenient.
Drawings
FIG. 1 is a schematic structural diagram of a detection device for fish kidney tissue cells according to example 1;
FIG. 2 is a side sectional view of a fixing ring and a second extension ring of the detecting device for fish kidney tissue cells in example 1;
FIG. 3 is a schematic structural view of a fixing ring and a second extension ring of the detection device for fish kidney tissue cells in example 1;
FIG. 4 is a sectional view showing the side structure of a base and a mounting plate of the detecting unit for fish kidney tissue cells according to example 2;
FIG. 5 is a sectional view of a top view of a mounting plate of the detecting apparatus for fish kidney tissue cells according to example 2.
In the figure: the microscope comprises a base 1, a groove 2, a first through hole 3, a first illuminating lamp 4, a rotating motor 5, an extension rod 6, a movable plate 7, a magnet piece 8, a first sliding groove 9, a placing plate 10, a first extension ring 11, a microscope body 12, a column 13, a fixing ring 14, a limiting rod 15, a first guide groove 16, a second through hole 17, a connecting rope 18, a second guide groove 19, a second extension ring 20, a movable ring 21, a second illuminating lamp 22 and a handle 23.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1
Referring to fig. 1-3, a detection device for fish kidney histiocyte comprises a base 1, wherein the left side of the outer wall of the top of the base 1 is connected with a stand column 13 through a fastening bolt, the top of the stand column 13 is connected with a microscope body 12 through a fastening bolt, the right side of the inner wall of the top of the base 1 is connected with a rotating motor 5 through a fastening bolt, the output shaft of the rotating motor 5 is connected with a placing plate 10 through a fastening bolt, the circle center of the top of the placing plate 10 and the outer wall of the top of the base 1 are both provided with a first perforation 3, the inner wall of the bottom of the base 1 is connected with a first illuminating lamp 4 through a fastening bolt, the placing plate 10 can be driven by the rotating motor 5 to rotate out of the bottom of the microscope body 12, fish kidney slice cells are placed at the first perforation 3 on the placing plate 10, the placing plate 10 is driven, first light 4 throws light on 3 departments of first perforation simultaneously, is convenient for observe and detects, makes things convenient for the dismouting of cell tissue, need not to adjust the sliced concrete position of cell at every turn, and is convenient high-efficient.
In the present invention, the opening positions of the microscope body 12, the first illuminating lamp 4 and the first through hole 3 are located in the same vertical direction, and the fixing clip is mounted on the top of the placing plate 10 along the opening of the first through hole 3.
Wherein, the bottom of base 1 right side outer wall is pegged graft and is had two extension rods 6, and the one end that two extension rods 6 extend to base 1 right side outer wall has same fly leaf 7 through fastening bolt connection, and the one end that two extension rods 6 extend to base 1 inside has same limiting plate through fastening bolt connection, and fly leaf 7 is close to one side of base 1 and is connected with magnet piece 8 through fastening bolt, and first spout 9 has been seted up at the top of fly leaf 7.
Wherein, the second spout has been seted up to the top outer wall of base 1, and place one side of board 10 bottom and have the slider through fastening bolt connection, the external diameter of slider and the internal diameter of first spout 9, the internal diameter looks adaptation of second spout, when needing dismouting cell section, can take out fly leaf 7, the limiting plate carries on spacingly to fly leaf 7, when screwing out and placing board 10, the slider slides in proper order in second spout and first spout 9, fly leaf 7 supports the bottom of screwing out and placing board 10.
Wherein, the circumference department of stand 13 has cup jointed retainer plate 14, and the outer wall of one side of retainer plate 14 is connected with movable ring 21 through fastening bolt.
Wherein, the circumference inner wall sliding connection of activity circle 21 has first extension circle 11, and the circumference outer wall sliding connection of activity circle 21 has the second to extend circle 20, and the bottom of the first 11 circumference inner walls that extend circle is connected with second light 22 through fastening bolt.
Wherein the bottom of the circumferential inner wall of the second extension ring 20 is connected with evenly distributed connecting ropes 18 through fastening bolts, and the top of the circumferential outer wall of the movable ring 21 is provided with second through holes 17 which are uniformly distributed, one end of the connecting rope 18 far away from the second extending ring 20 passes through the second through holes 17 to be fixedly connected with the top of the first extending ring 11, when the placing plate 10 is loaded and reset to the bottom of the microscope body 12, turning the fixed ring 14 so that the movable ring 21 rotates to the top of the placing plate 10, pulling downward to unfold the first extension ring 11, the first extension ring 11 moves downward while its top pulls the second extension ring 20 through the connection string 18, so that the second extension ring 20 is unfolded upward, the second illumination lamp 22 illuminates the placing board 10 at the time of inspection and observation, meanwhile, the second extension ring 20 and the movable ring 21 shield the surrounding interference light, so that the detection device is practical, flexible and convenient, and the detection precision is ensured.
Wherein, evenly distributed's second guide way 19 is seted up to the circumference inner wall that the second extended circle 20, and evenly distributed's first guide way 16 is seted up to the circumference outer wall of activity circle 21, the circumference inner wall bottom that the second extended circle 20 is provided with evenly distributed's first guide block, the top of activity circle 21 circumference outer wall is provided with evenly distributed's second guide block, first guide way 16 and second guide way 19 are led it when the second extended circle 20 and the first extension circle 11 slip expand.
Wherein, the bottom of the outer wall of 11 right sides of first extension circle has handle 23 through fastening bolt connection, and the bottom circumference department of activity circle 21 is fixed with the magnet piece, and the cross section of the first 11 sides of extension circle is L type structure, and it stirs first extension circle 11 to detect to accomplish backward, and the bottom of activity circle 21 contacts and passes through the magnet piece absorption fixedly with the bottom of the first 11 that extend circle, and the second extends circle 20 and resets to the circumference department of activity circle 21 because of gravity downstream this moment, and is practical convenient.
The working principle is as follows: the placing plate 10 can be driven by the rotating motor 5 to rotate out of the bottom of the microscope body 12, fish kidney slice cells are placed at the first through hole 3 on the placing plate 10, the placing plate 10 is driven by the rotating motor 5 to rotate to the bottom of the microscope body 12 for observation after fixation is completed, meanwhile, the first through hole 3 is illuminated by the first illuminating lamp 4, observation and detection are facilitated, disassembly and assembly of cell tissues are facilitated, each adjustment of the specific position of the cell slice is not needed, convenience and high efficiency are achieved, when the cell slice is required to be disassembled and assembled, the movable plate 7 can be pulled, the movable plate 7 is limited by the limiting plate, when the placing plate 10 is screwed out, the sliding block sequentially slides in the second sliding groove and the first sliding groove 9, the movable plate 7 supports the bottom of the screwed-out placing plate 10, when the placing plate 10 is assembled and reset to the bottom of the microscope body 12, the fixing ring 14 is rotated to enable the movable ring 21 to rotate, the first extension circle 11 of downward pulling expansion, its top is through connecting rope 18 pulling second extension circle 20 when first extension circle 11 moves down, make the second extend circle 20 and upwards expand, second light 22 throws light on placing board 10 when detecting the observation, second extension circle 20 and movable ring 21 shelter from the interference light on every side simultaneously, it is practical nimble convenient, and guarantee the precision of detection, first guide way 16 and second guide way 19 lead it when second extension circle 20 and first extension circle 11 slide expansion, stir first extension circle 11 after the detection is accomplished, the bottom of movable ring 21 contacts with the bottom of first extension circle 11 and adsorbs fixedly through the magnet piece, second extension circle 20 resets to the circumference department of movable ring 21 because of gravity downward movement this moment, and is practical and convenient.
Example 2
Referring to fig. 4-5, compared with embodiment 1, in the present embodiment, a groove 2 is formed in one side of a placing plate 10, a limiting rod 15 is connected to the bottom of one side of a column 13 close to the placing plate 10 through a fastening bolt, and the outer diameter of the limiting rod 15 is matched with the inner diameter of the groove 2.
The working principle is as follows: when the rotating motor 5 drives the placing plate 10 to rotate and reset, the limiting rod 15 moves in the groove 2 and limits the reset of the placing plate 10.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (10)

1. The utility model provides a detection apparatus for be used for fish kidney histiocyte, includes base (1), the left side of base (1) top outer wall is equipped with stand (13), and the top of stand (13) is equipped with microscope body (12), its characterized in that, the right side of base (1) top inner wall is equipped with rotating electrical machines (5), and the output shaft of rotating electrical machines (5) is equipped with places board (10), place the centre of a circle department at board (10) top and the top outer wall of base (1) and all seted up first perforation (3), and base (1) bottom inner wall is equipped with first light (4).
2. The fish kidney tissue cell detection device according to claim 1, wherein the microscope body (12), the first illumination lamp (4) and the first through hole (3) are arranged in the same vertical direction, and a fixing clip is arranged on the top of the placement plate (10) along the opening of the first through hole (3).
3. The detection device for fish kidney tissue cells according to claim 2, wherein two extension rods (6) are inserted into the bottom of the outer wall of the right side of the base (1), one end of each of the two extension rods (6) extending to the outer wall of the right side of the base (1) is provided with the same movable plate (7), one end of each of the two extension rods (6) extending to the inside of the base (1) is provided with the same limiting plate, one side of each of the movable plates (7) close to the base (1) is provided with a magnet piece (8), and the top of each of the movable plates (7) is provided with the first sliding groove (9).
4. The fish kidney tissue cell detection device according to claim 3, wherein a second sliding groove is formed in the outer wall of the top of the base (1), a sliding block is arranged on one side of the bottom of the placing plate (10), and the outer diameter of the sliding block is matched with the inner diameter of the first sliding groove (9) and the inner diameter of the second sliding groove.
5. The fish kidney tissue cell detection device according to claim 4, wherein a fixed ring (14) is sleeved at the circumference of the upright post (13), and a movable ring (21) is arranged on the outer wall of one side of the fixed ring (14).
6. The fish kidney tissue cell detection device according to claim 5, wherein a first extension ring (11) is slidably connected to the inner circumferential wall of the movable ring (21), a second extension ring (20) is slidably connected to the outer circumferential wall of the movable ring (21), and a second illuminating lamp (22) is arranged at the bottom of the inner circumferential wall of the first extension ring (11).
7. The fish kidney tissue cell detection device as claimed in claim 6, wherein the bottom of the circumferential inner wall of the second extension ring (20) is provided with uniformly distributed connection ropes (18), the top of the circumferential outer wall of the movable ring (21) is provided with uniformly distributed second through holes (17), and one end of the connection rope (18) far away from the second extension ring (20) penetrates through the second through holes (17) to be fixedly connected with the top of the first extension ring (11).
8. The fish kidney tissue cell detection device according to claim 7, wherein the second extension ring (20) is provided with uniformly distributed second guide grooves (19) on the inner circumferential wall, the movable ring (21) is provided with uniformly distributed first guide grooves (16) on the outer circumferential wall, the uniformly distributed first guide blocks are arranged at the bottom of the inner circumferential wall of the second extension ring (20), and the uniformly distributed second guide blocks are arranged at the top of the outer circumferential wall of the movable ring (21).
9. The fish kidney tissue cell detection device as recited in claim 8, wherein a handle (23) is provided at the bottom of the outer wall of the first extension ring (11), a magnet piece is fixed at the bottom circumference of the movable ring (21), and the cross section of the side of the first extension ring (11) is an L-shaped structure.
10. The fish kidney tissue cell detection device according to claim 9, wherein one side of the placement plate (10) is provided with a groove (2), the bottom of the upright column (13) close to the placement plate (10) is provided with a limiting rod (15), and the outer diameter of the limiting rod (15) is matched with the inner diameter of the groove (2).
CN202010414416.3A 2020-05-15 2020-05-15 Detection device for fish kidney tissue cells Pending CN111534424A (en)

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CN202010414416.3A CN111534424A (en) 2020-05-15 2020-05-15 Detection device for fish kidney tissue cells

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CN202010414416.3A CN111534424A (en) 2020-05-15 2020-05-15 Detection device for fish kidney tissue cells

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Cited By (1)

* Cited by examiner, † Cited by third party
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CN114062662A (en) * 2022-01-14 2022-02-18 迈赛凯尔(山东)生命科学有限公司 Tissue cell activity detector device

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