CN216208035U - Low temperature box of supplementary ultrasonic wave cell disruption appearance - Google Patents

Abstract

The utility model relates to the technical field of low-temperature boxes and discloses a low-temperature box for assisting an ultrasonic cell disruption instrument, which comprises a box body, wherein through openings are formed in the upper part and the lower part of the box body; the bottom of the box body is detachably connected with a sealing element; clean support piece has been cup jointed in the inside slip of box body, the backup pad has been cup jointed in the inside slip of box body, a plurality of jack has been seted up in the backup pad, a plurality of the rubber ring is all fixed the cover in the inside of jack and is equipped with. The utility model provides a low-temperature box of an auxiliary ultrasonic cell disruption instrument, which is beneficial to observing the operation of a sample in an EP (ultraviolet) tube in the low-temperature box in real time.

Description

Low temperature box of supplementary ultrasonic wave cell disruption appearance

Technical Field

The utility model relates to the field of low-temperature boxes, in particular to a low-temperature box for assisting an ultrasonic cell disruption instrument.

Background

The ultrasonic cell disruptor is an instrument which is required to be used when protein is extracted in routine biochemical experiments such as ChIP, WB and the like, and is required to be carried out at a low temperature when the ultrasonic cell disruptor is used for preventing protein degradation, and ice bath, ice water bath or low-temperature water bath are the most common methods for keeping the sample at a low temperature in operation. However, the ice box on the market cannot be used because it cannot observe the position of the ultrasonic probe on the specimen processing tube in real time.

At present, the ice bath device in laboratory is mostly the simple and easy device that the experimenter made, if use used all kinds of EP pipe of experiment, centrifuging tube to scatter on the ice surface, this type of operation often because directly place on the ice surface not fixed and easy the slip, often causes irreparable influence for the experiment.

CN211913816U discloses a laboratory low temperature box, which comprises a box body and a sample supporting plate, wherein the upper part of the box body is open, the sample supporting plate is detachably mounted on the inner wall of the box body to make the surface of the supporting plate parallel to the bottom surface of the box body; the sample supporting plate is provided with a tube area and a plate area, the tube area is provided with a plurality of EP tube through holes for placing EP tubes, the plate area is provided with one or more micropore plate supporting parts, the micropore plate supporting parts comprise micropore plate through holes and supporting components, and the supporting components are arranged at the bottoms of the micropore plate through holes and are connected with the main body of the sample supporting plate.

CN214333142U discloses small-size refrigeration ice box convenient to controlled temperature, place the district including refrigeration ice box main part and EP, refrigeration ice box main part one side is provided with the side box, refrigeration ice box main part front side is provided with the front panel, is provided with temperature liquid crystal display on the front panel, temperature liquid crystal display one side is provided with the switch, refrigeration ice box main part right side below is provided with refrigerating plant, the refrigerating plant outside is provided with the power, refrigeration ice box main part top is provided with the EP is placed the district, the EP is placed the inside EP pipe area that is provided with in district, EP pipe area left side is provided with a plurality of EP pipe area.

In order to solve the above problems, the present application provides a cryocassette for assisting an ultrasonic cell disruption apparatus.

SUMMERY OF THE UTILITY MODEL

Objects of the utility model

In order to solve the technical problems in the background art, the utility model provides a low-temperature box for an auxiliary ultrasonic cell disruption instrument.

(II) technical scheme

In order to solve the problems, the utility model provides a low-temperature box for assisting an ultrasonic cell disruption instrument, which comprises a box body, wherein through openings are formed in the upper part and the lower part of the box body;

the bottom of the box body is detachably connected with a sealing element;

clean support piece has been cup jointed in the inside slip of box body, the backup pad has been cup jointed in the inside slip of box body, a plurality of jack has been seted up in the backup pad, a plurality of the rubber ring is all fixed the cover in the inside of jack and is equipped with.

Preferably, the sealing element comprises a bottom plate and a threaded ring, the bottom plate is located at the bottom of the box body, the threaded ring is fixedly installed at the top of the bottom plate, and the threaded ring is in threaded sleeve connection with the box body.

Preferably, the cleaning support piece comprises a rubber ring and a supporting rod, the rubber ring is movably sleeved inside the box body, one end of the supporting rod is fixedly connected with the top of the rubber ring, and the supporting plate is placed on the top end of the supporting rod.

Preferably, the diameter values of a plurality of the jacks are different.

Preferably, a pull ring is fixedly mounted at the top of the support plate.

Preferably, the support plate is provided with a sequence number.

The technical scheme of the utility model has the following beneficial technical effects:

when an experimenter operates, water or isopropanol is injected into the box body, the box body is placed in a refrigerator for precooling to 0 ℃ before use, and then the sample supporting plate is placed at the top of the supporting rod, so that the experimenter can conveniently and orderly place the EP pipe in the rubber ring, the EP pipe is not easy to overturn, and the orderliness during low-temperature operation is kept;

the jacks are provided with various sizes, EP pipes with different sizes are often used according to different volumes in the experiment process, and the EP pipes with various sizes are placed and used;

because the sealing member sets up with the box body split, do benefit to and clear up sealing member and box body, through pulling branch, branch drives the rubber circle and removes in vertical direction to reached and do benefit to the effect of clearing up the box body inner wall.

Drawings

FIG. 1 is a schematic structural diagram of a cryobox of an ultrasonic assisted cell disruption apparatus according to the present invention.

Fig. 2 is a schematic diagram of a split structure of a cryobox of an ultrasonic-assisted cell disruption apparatus according to the present invention.

Reference numerals: 1. a box body; 2. a seal member; 21. a base plate; 22. a thread ring; 3. cleaning the support; 31. a rubber ring; 32. a strut; 4. a support plate; 5. a jack; 6. a rubber ring; 7. and (4) a pull ring.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings in conjunction with the following detailed description. It should be understood that the description is intended to be exemplary only, and is not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.

As shown in fig. 1-2, the cryobox of the ultrasonic cell disruption assisting apparatus provided by the present invention comprises a box body 1, wherein the sidewall of the box body 1 is made of transparent material, and the box body 1 has through holes formed at the upper and lower parts;

the bottom of the box body 1 is detachably connected with a sealing element 2;

clean support piece 3 has been cup jointed in the inside slip of box body 1, and backup pad 4 has been cup jointed in the inside slip of box body 1, has seted up a plurality of jack 5 on backup pad 4, and the rubber ring 6 is all fixed the cover in the inside of a plurality of jack 5 and is equipped with.

In an optional embodiment, the sealing member 2 includes a bottom plate 21 and a thread ring 22, the bottom plate 21 is located at the bottom of the box body 1, the thread ring 22 is fixedly installed at the top of the bottom plate 21, the thread ring 22 is in threaded sleeve connection with the box body 1, and then the bottom plate 21 is separated from the box body 1, so that the effect of facilitating disassembly and further cleaning the bottom plate is achieved, and the phenomenon that the included angle between the inner bottom surface and the side wall in the prior art is not favorable for cleaning is avoided.

In an optional embodiment, clean support piece 3 includes rubber circle 31 and branch 32, rubber circle 31 activity cup joints the inside at box body 1, the one end of branch 32 and the top fixed connection of rubber circle 31, backup pad 4 is placed on the top of branch 32, the quantity of branch 32 is two, and two branches 32 symmetry sets up the top at rubber circle 31, pull branch 32 at vertical direction and drive rubber circle 31, so as to play the effect of scraping to the inner wall of box body 1, thereby reached the effect of being convenient for clear up.

In an alternative embodiment, the diameter values of the plurality of sockets 5 are different, and the diameter values of the plurality of rubber rings 6 are different and are adapted to the corresponding sockets 5 to adapt to EP pipes with different diameter values.

In an alternative embodiment, a pull ring 7 is fixedly mounted on the top of the support plate 4, and the support plate 4 is moved by the pull ring 7.

In an alternative embodiment, the support plate 4 is provided with serial numbers, and the serial numbers are used for marking the EP tubes with different diameters, so as to facilitate the placement of different EP tubes.

In the utility model, when an experimenter operates, water or isopropanol is injected into the box body 1, the box body is placed in a refrigerator for precooling to 0 ℃ before use, and then the sample supporting plate 4 is placed at the top of the supporting rod 32, so that the experimenter can conveniently and orderly place the EP pipe in the rubber ring 6, the EP pipe is not easy to overturn, and the orderliness in low-temperature operation is kept; the jacks 5 are provided with various sizes, EP pipes with different sizes are often used according to different volumes in the experiment process, and the EP pipes with various sizes are placed and used; because sealing member 2 sets up with 1 split of box body, do benefit to and clear up sealing member 2 and box body 1, through pulling branch 32, branch 32 drives rubber ring 31 and removes in vertical direction to reached and do benefit to the effect of clearing up 1 inner wall of box body.

It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the utility model and are not to be construed as limiting the utility model. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.

Claims (6)

1. The utility model provides an auxiliary ultrasonic wave cell disruption appearance's low temperature box, includes box body (1), its characterized in that: the upper part and the lower part of the box body (1) are provided with through holes;

the bottom of the box body (1) is detachably connected with a sealing element (2);

clean support piece (3) have been cup jointed in the inside slip of box body (1), backup pad (4) have been cup jointed in the inside slip of box body (1), a plurality of jack (5), a plurality of have been seted up on backup pad (4) the inside of jack (5) is all fixed the cover and is equipped with rubber ring (6).

2. The cryocassette of an assisted ultrasonic cytoclasis instrument as claimed in claim 1, characterized in that said sealing member (2) comprises a bottom plate (21) and a threaded ring (22), said bottom plate (21) is located at the bottom of the cassette body (1), said threaded ring (22) is fixedly installed at the top of the bottom plate (21), said threaded ring (22) is in threaded socket joint with the cassette body (1).

3. The cryobox of an auxiliary ultrasound cytoclasis instrument as claimed in claim 1, characterized in that said cleaning support member (3) comprises a rubber ring (31) and a support rod (32), said rubber ring (31) is movably sleeved inside the box body (1), one end of said support rod (32) is fixedly connected with the top of the rubber ring (31), said support plate (4) is placed on the top end of the support rod (32).

4. The cryobox of an assisted ultrasonic cytoclasser according to claim 1, characterized in that the diameter values of several of said receptacles (5) are different.

5. The cryobox of an assisted ultrasonic cell disruption instrument of claim 1, wherein a pull ring (7) is fixedly mounted on the top of said support plate (4).

6. The cryocassette of claim 1, wherein the support plate (4) is provided with serial numbers.

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