CN216144810U - Particle detection device - Google Patents

Abstract

The embodiment of the application discloses particle detection device, including the base plate with set up at least one detecting element on the base plate, at least one detecting element includes: a detection cell, wherein the detection cell can contain a sample liquid; the intercommunication the appearance portion of advancing of detection pond, the appearance portion of advancing can with liquid drive arrangement sealing connection, liquid drive arrangement with the cooperation of appearance portion is used for making sample liquid advances out the detection pond. This application particle detection device's sample introduction portion can seal cooperation liquid drive arrangement and lead into and derive sample liquid by the detection cell fast.

Description

Particle detection device

Technical Field

The specification relates to the technical field of biological detection, in particular to a particle detection device.

Background

The particle detection device can be used for biological sample detection, and utilizes a specific marker to detect or separate a target analyte in a biological sample. The particle detection device can use an applicable marker according to different target analytes in a sample, and has wide application in the fields of clinical examination, drug analysis, environmental monitoring and the like.

SUMMERY OF THE UTILITY MODEL

One of the embodiments of the present specification provides a particle detecting apparatus including a substrate and at least one detecting unit disposed on the substrate, the at least one detecting unit including: a detection cell, wherein the detection cell can contain a sample liquid; the intercommunication the appearance portion of advancing of detection pond, the appearance portion of advancing can with liquid drive arrangement sealing connection, liquid drive arrangement with the cooperation of appearance portion is used for making sample liquid advances out the detection pond.

In some embodiments, the sample introduction part comprises a sample introduction channel and a sample introduction port communicated with the detection cell through the sample introduction channel.

In some embodiments, the inner aperture of the sample channel gradually decreases in a direction from the sample inlet to the detection cell.

In some embodiments, the inner profile of the sample channel is tapered.

In some embodiments, the base plate comprises a first plate body and a second plate body; the detection pool is arranged on the second plate body; the sample inlet is arranged on the first plate body; the sampling channel is arranged on the first plate body, or the sampling channel is arranged on the first plate body and the second plate body.

In some embodiments, the axis of the sample channel forms an angle of 20-60 ° with the first plate body.

In some embodiments, the first plate body and the second plate body are arranged in a sealed manner, the detection cell is located between the first plate body and the second plate body, and the sample injection channel is communicated with the detection cell and the sample injection port.

In some embodiments, the shape of the injection port in a cross section parallel to the first plate body is elliptical or circular.

In some embodiments, the minor axis length of the elliptical cross-section of the injection port is 0.5-4 mm.

In some embodiments, the sample inlet protrudes out of the first plate body.

In some embodiments, the height of the sample inlet protruding from the first plate body is 0.5-3 mm.

In some embodiments, the length of the sample channel is greater than 1 mm.

In some embodiments, the sample introduction part further comprises an elastic sealing member provided with a through hole, the elastic sealing member being mounted within the sample introduction channel and/or on the sample introduction port.

In some embodiments, the depth of the detection cell is less than 2 mm.

In some embodiments, a marker is pre-embedded in the sample introduction part and/or the detection cell, and at least part of the marker is mixed into the sample liquid entering and exiting the detection cell through cooperation of the liquid driving device and the sample introduction part.

One of the embodiments of the present application further provides an operation method of the above-mentioned particle detection apparatus, the method including: introducing a sample liquid into the detection cell through the sample introduction part; a liquid driving device which is hermetically connected with the sample introduction part provides liquid driving force, so that at least part of the sample liquid repeatedly enters and exits the detection cell; and driving the sample liquid to flow into the detection cell again through the auxiliary driving device.

In some embodiments, the driving force output part of the liquid driving device is inserted into and presses the sample injection part, so that the liquid driving device is in sealed connection with the sample injection part.

In some embodiments, the liquid driving device drives at least a portion of the sample liquid to repeatedly enter and exit the detection cell at least 1 time, so that at least a portion of the markers pre-embedded in the sample introduction part or the detection cell is mixed with the sample liquid.

In some embodiments, the liquid driving device is a device with positive and negative pressure control; the device with positive and negative pressure control is one of a pipette, a syringe and a skin blower.

Drawings

The present description will be further explained by way of exemplary embodiments, which will be described in detail by way of the accompanying drawings. These embodiments are not intended to be limiting, and in these embodiments like numerals are used to indicate like structures, wherein:

FIG. 1 is a schematic perspective view of a particle detection apparatus according to some embodiments of the present disclosure;

fig. 2 is a front view of a first plate body of a particle detection device provided with a plurality of detection units according to some embodiments of the present disclosure;

FIG. 3 is a front view of a particulate detection apparatus according to some embodiments of the present description;

FIG. 4 is a cross-sectional view taken along line A-A of FIG. 2;

FIG. 5 is an enlarged schematic view of B in FIG. 4;

FIGS. 6-11 are cross-sectional views of a sample introduction portion of a particulate detection device according to some embodiments of the present description;

FIG. 12 is a cross-sectional view of a particulate detection apparatus according to some embodiments of the present description;

FIG. 13 is a cross-sectional view of a particulate detection apparatus according to some embodiments of the present description;

in the figure: 100-a first plate body, 200-a second plate body, 300-a detection unit, 310-a detection cell, 320-a sample introduction part, 321-a sample introduction port, 322-a sample introduction channel, 323-a flange, 324-an elastic sealing member, 325-a plunger, 326-a plunger cavity, 326 a-a first plunger cavity, 326 b-a second plunger cavity, 327-a partition flap, 328-a sealing plug, 329-a mixing channel and 330-an exhaust port.

Detailed Description

Reference will now be made in detail to exemplary embodiments or implementations, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present application. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present application, as detailed in the appended claims.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this application and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

It should be understood that the use of "first," "second," and similar terms in the description and claims do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. Also, the use of the terms "a" or "an" and the like do not denote a limitation of quantity, but rather denote the presence of at least one. Unless otherwise indicated, "front", "rear", "lower" and/or "upper" and the like are for convenience of description and are not limited to one position or one spatial orientation. The word "comprising" or "comprises", and the like, means that the element or item listed as preceding "comprising" or "includes" covers the element or item listed as following "comprising" or "includes" and its equivalents, and does not exclude other elements or items.

Depending on the label and the target analyte in the sample, the application scenario of the particle detection apparatus may generally include: 1) and (5) performing clinical examination. For example, cell identification, cell counting, cell viability detection, cell cycle detection, apoptosis detection, etc. using the binding of specific dyes to cells; the antibody or antigen is marked by using substances related to chemiluminescence, after the antibody or antibody reacts with the antigen or antibody to be detected, the free chemiluminescent marker is separated and added into other related substances of a chemiluminescent system to generate chemical reaction so as to carry out quantitative or qualitative immunoassay of the antigen or antibody. 2) And (5) analyzing the medicine. For example, a specific detection reagent is used as a label to be mixed with a biological sample such as a blood sample, a urine sample, etc. to detect a drug component in the biological sample and its concentration. 3) And (5) monitoring the environment. For example, detection and quantification of microorganisms in environmental biological samples is performed using specific dyes as labels that undergo a detectable color change in the presence of one or more microorganisms.

In order to observe and/or detect a trace amount of sample in the particle detection device, the thickness of the detection cell and the inner diameter of the flow channel of the particle detection device are generally between several tens and several hundreds of micrometers. Once the sample liquid enters the detection cell of the particle detection apparatus, it is difficult to draw out the sample liquid. Therefore, it is desirable to provide a particle detection device that can rapidly draw or suck a sample liquid from a detection cell. The particle detection device in some embodiments of the present application realizes rapid leading-out or sucking-up of sample liquid from the detection cell by providing the sample injection portion capable of being hermetically fitted with the liquid driving device.

The particle detection device and the operation method thereof according to the embodiments of the present application will be described in detail below with reference to fig. 1 to 13. It should be noted that the following examples are only for explaining the present application and do not constitute a limitation to the present application.

In some embodiments, the particle detection device may include a substrate and one or more detection units 300 disposed on the substrate, and each detection unit 300 may include a detection cell 310 and a sample introduction part 320 communicating with the detection cell 310. The sample introduction part 320 is a main structure for guiding and leading out the sample liquid by matching with the liquid driving device, and the particle detection device can guide in the sample liquid to be detected to the detection cell 310 through the sealed matching of the sample introduction part 320 and the liquid driving device, and can guide out or absorb the sample liquid from the detection cell 310 through the sample introduction part 320 and the liquid driving device.

The substrate is the main structure of the particle detection device. The substrate can be an integrated structure or a multi-layer connection structure. In some embodiments, for ease of manufacturing, as shown in fig. 1-2, the substrate may include a first board 100 and a second board 200, with at least a portion of the structure of the detection unit 300 disposed between the first board 100 and the second board 200. Further, in some embodiments, the first plate 100 and the second plate 200 of the substrate are hermetically sealed. For example, the first plate body 100 and the second plate body 200 may be hermetically connected by bonding, laser welding, ultrasonic welding, plasma treatment, or the like.

The inspection unit 300 is a collection of structures or components on a substrate that cooperate to complete a predetermined inspection. In some embodiments, one detection unit 300 may be disposed on the substrate. In some embodiments, as shown in FIG. 3, a plurality of detection units 300 may be disposed on the substrate, such that the particle detection apparatus can perform a plurality of sample detections, or a plurality of sample detections at the same time. Considering that the inspection apparatus is a disposable consumable, in order to facilitate the calculation of the experimental design and the control of the consumption of the consumable, and considering the convenience of use and the limitation of the size of the substrate, 1 to 10 inspection units 300 may be preferably disposed on the substrate.

The detection cell 310 can contain a sample solution, and is a main place for performing optical detection. The sample solution is collected in the detection cell 310, and the optical detection device detects the sample solution through the detection cell 310. In some embodiments, the detection cell 310 is disposed on the second plate 200, and the detection cell 310 located between the first plate 100 and the second plate 200 forms a relatively closed detection environment through the sealing connection between the first plate 100 and the second plate 200. In some embodiments, the first plate body 100 and/or the second plate body 200 are configured to allow light to be transmitted into the detection cell 310 and to allow an optical detection device to detect the light transmitted out of the detection cell 310. In some embodiments, the depth of the detection cell 310 is preferably less than 2 mm. The preferable depth of the detection cell 310 can avoid the overlap of the objects to be detected in the sample liquid in the depth direction of the detection cell 310 as much as possible, thereby ensuring the detection accuracy.

The sample liquid to be measured is introduced into and discharged/sucked out of the detection cell 310 through the sample introduction part 320. In some embodiments, the sample introduction portion 320 can be in sealed connection with a liquid driving device, the cooperation of the liquid driving device and the sample introduction portion 320 being used for moving the sample liquid into and out of the detection cell 310. Specifically, the sample introduction part 320 is hermetically connected to the liquid driving device, so that the liquid driving force provided by the liquid driving device can effectively act on the sample liquid entering the detection unit 300, and the sample liquid can repeatedly enter and exit the detection cell 310 under the action of the liquid driving force. In some embodiments, the liquid drive device may be one of a pipette, a syringe, and a skin-blow. Preferably, the liquid drive device is a pipette. Specifically, the liquid driving device can have the function of accurately measuring the sample liquid, and can provide liquid driving force to realize the introduction and the discharge of the sample liquid; the liquid driving device may not have a function of accurately measuring the sample liquid, but may provide a liquid driving force to realize the introduction and the discharge of the sample liquid.

In some embodiments, the sample inlet 320 includes a sample channel 322 and a sample inlet 321, and the sample inlet 321 is directly or indirectly communicated with the detection cell 310 through the sample channel 322. The sample inlet 321 and the sample channel 322 may allow a driving force output portion of the liquid driving device to be inserted therein and form a seal to the driving force output portion. For example, the liquid driving device is a pipette, the driving force output portion is a tip, and the sample inlet 321 and the sample channel 322 may allow the tip of the pipette to be inserted therein and form a seal with the tip.

To achieve the sealing effect, the sample injection part 320 may be attached to the driving force output part of the liquid driving device. Specifically, the inner contour surface of at least part of the section of the sample feeding channel can be attached to the outer wall of the driving force output part of the liquid driving device; furthermore, the whole inner contour surface of the sample feeding channel can be attached to the outer wall of the driving force output part of the liquid driving device, and the inner contour of the sample feeding channel can be attached to the outer wall of the driving force output part of the liquid driving device.

In some embodiments, the inner profile of the sample channel 322 is circular tubular. Specifically, for the liquid driving apparatus with the outer wall of the driving force output part being a cylindrical surface, the inner contour surface of the sample injection channel 322 in a circular tube shape can be attached to the outer wall of the driving force output part of the liquid driving apparatus close to the output port. For example, the outer wall of the injector head of the injector is a cylindrical surface, and the inner contour surface of the sample introduction channel 322 in a circular tube shape can fit the outer cylindrical surface of the injector head of the injector near the injection port.

In some embodiments, as shown in fig. 4, the inner diameter of the sample channel 322 gradually decreases in the direction from the sample inlet 321 to the detection cell 310. In some embodiments, further, the inner profile of the sample channel 322 is tapered. Specifically, for the liquid driving apparatus in which the outer wall of the driving force output portion is a conical surface, the sample injection channel 322 having a gradually decreasing inner diameter, especially when the inner contour surface thereof is in a conical shape, may be attached to the outer wall of the driving force output portion of the liquid driving apparatus near the output port. For example, the outer wall of the tip of the pipette is a conical surface, and the conical inner contour surface of the sample injection channel 322 can be attached to the outer conical surface of the tip of the pipette close to the liquid outlet. Preferably, a conical inner profile sample channel 322 is used.

The length of the sample channel 322 can affect the fit and sealing effect of the sample inlet portion 320 and the driving force output portion of the liquid driving device. It should be noted that the length of the sample channel 322 refers to the shortest straight distance on the inner contour surface of the sample channel 322, as shown by the length D in fig. 5. In some embodiments, the length of the sample channel 322 is greater than 1 mm.

The position and arrangement of the sample feeding channel 322 can affect the fitting and sealing effect of the sample feeding part 320 and the driving force output part of the liquid driving device.

In some embodiments, as shown in fig. 4, sample inlet 321 and sample channel 322 can be disposed on first plate body 100. In some embodiments, the sample inlet 321 may be disposed on the first plate body 100; sample channels 322 may be disposed on the first plate body 100 and the second plate body 200. Specifically, a part of the sample channel 322 may be formed on the first plate 100, the other part of the sample channel 322 may be formed on the second plate 200, and the first plate 100 and the second plate 200 are connected to form the complete sample channel 322. In some embodiments, to ensure the length of the sample channel 322, thereby improving the sealing effect, the sample channel 322 is obliquely disposed on the first plate 100. Specifically, as shown in fig. 5, an acute included angle θ is formed between the axis of the sample inlet channel 322 and the first plate 100 (the opening surface of the sample inlet 321/the upper surface of the substrate). In some embodiments, the included angle θ is preferably 20-60 °.

In some embodiments, as shown in fig. 10, a sample inlet 321 and a sample channel 322 may be disposed between the first plate body 100 and the second plate body 200. In some embodiments, the axis of the sample channel 322 can be perpendicular to the side wall, or the axis of the sample channel 322 can be oblique to the side wall, on the face where the sample inlet 321 opens, i.e., the substrate opens the corresponding side wall of the sample inlet 321. Specifically, when the sample inlet 321 and the sample channel 322 are disposed between two plate bodies, the substrate has sufficient depth in the length and width directions, so that the axis of the sample channel 322 is perpendicular to or inclined to the sidewall of the substrate, and the channel length can be sufficient to meet the requirement of sealing.

In some embodiments, as shown in fig. 11, the sample inlet 321 and the sample channel 322 may be disposed on the second plate body 200, on the opening surface of the sample inlet 321, i.e. on the corresponding sidewall of the substrate on which the sample inlet 321 is opened, and the axis of the sample channel 322 may be inclined to the sidewall.

The sample inlet 321 is disposed in a manner that can affect the adhesion and sealing effect between the sample inlet 320 and the driving force output portion of the liquid driving apparatus. In some embodiments, the sample inlet 321 protrudes from the opening surface of the sample inlet 321 to ensure the length of the sample channel 322, thereby improving the sealing effect. For example, as shown in fig. 6, the sample inlet 322 is obliquely disposed on the first plate 100, and the sample inlet 321 protrudes from the first plate 100 through the flange 323 of the sample inlet 320, so as to extend the length of the sample inlet 322. For example, as shown in fig. 8, the sample inlet 322 is vertically disposed on the first plate 100, and the sample inlet 321 protrudes from the first plate 100 through the flange 323 of the sample inlet 320. In some embodiments, the sample inlet 321 preferably protrudes from the first plate 100; the height H of the sample inlet 321 protruding from the first plate 100 is 0.5-3 mm.

The shape of the sample inlet 321 can affect the fit and sealing effect of the sample inlet 320 and the driving force output part of the liquid driving device. In some embodiments, the sample inlet 321 is elliptical in the open face of the sample inlet 321. For example, as shown in fig. 1 and 2, the sample inlet 321 is opened on the first plate body 100 (upper surface of the substrate), and the shape of the sample inlet 321 on a cross section parallel to the first plate body 100 is an ellipse. Specifically, the sample inlet 321 with the elliptical cross section is suitable for the situation that the sample channel 322 is inclined to the opening surface of the sample inlet 321. In some embodiments, the minor axis of the elliptical shaped injection port 321 is between 0.5mm and 4mm in the opening surface of the injection port 321. In some embodiments, the sample inlet 321 is circular on the open face of the sample inlet 321. For example, the sample inlet 321 is opened on the first plate body 100, and the shape of the sample inlet 321 on the cross section parallel to the first plate body 100 is circular. Specifically, the sample inlet 321 with a circular cross section is suitable for the situation that the sample channel 322 is perpendicular to the opening surface of the sample inlet 321. In some embodiments, the diameter of the circular cross-section of the injection port 321 is between 0.5mm and 4mm on the opening surface of the injection port 321.

In some embodiments, the sample introduction part 320 can also be made to fit and seal the driving force output part of different types of liquid driving devices by providing a detachable elastic sealing member 324 in the sample introduction channel 322 and/or at the sample introduction port 321. After the driving force output part of the liquid driving device is inserted into the through hole of the elastic sealing element, the inner wall of the through hole of the elastic sealing element deforms to be attached to the outer wall of the driving force output part in a sealing mode, and the purpose of adapting to the driving force output parts of different types of liquid driving devices is achieved. In some embodiments, the sample introduction part 320 further comprises an elastic sealing member 324, the elastic sealing member 324 is provided with a through hole, and the elastic sealing member 324 is installed in the sample introduction channel 322 and/or on the sample introduction port 321. For example, as shown in fig. 7, an elastic sealing member 324 is disposed in the sample channel 322; as shown in fig. 8, elastic sealing members 324 are disposed in the sample inlet channel 322 and the sample inlet 321; as shown in fig. 9, an elastic sealing member 324 protruding from the first plate 100 is disposed in the sample inlet channel 322 and on the sample inlet 321.

Markers may be embedded in each detection unit 300. In some embodiments, the label is pre-embedded in the sample injection part 320 and/or the detection cell 310, and at least part of the label is mixed into the sample liquid entering and exiting the detection cell 310 by the cooperation of the liquid driving device and the sample injection part 320. Specifically, by the liquid flow of the sample liquid into and out of the detection cell 310, part or all of the markers are mixed into the sample liquid, and a sufficient and uniform mixing effect is achieved.

In some embodiments, the detection unit 300 may further include an exhaust port 330 in communication with the detection cell 310. Specifically, the liquid driving device is hermetically connected to the sample injection portion 320, and when the sample liquid is driven to enter and exit the detection cell 310, the gas outlet 330 is used for ventilating the detection cell 310 to balance the gas pressure inside and outside the detection cell 310.

The application also discloses a particle detection device, and the particle detection device is internally provided with a liquid driving device communicated with the detection pool, samples are introduced through the liquid driving device, and sample liquid is rapidly and repeatedly led out and led in from the detection pool. The particle detection device includes a substrate and one or more detection cells 300 disposed within the substrate. In some embodiments, each of the detecting units 300 comprises a detecting cell 310 capable of receiving a sample solution and a sample injecting part 320 communicated with the sample injecting cell, wherein the sample injecting part 320 comprises a liquid driving device, the liquid driving device is fixed on the substrate, and the liquid driving device is used for driving at least a part of the sample solution to repeatedly enter and exit the detecting cell 310.

The particle detection device can adopt a negative pressure sample injection mode. Specifically, the liquid driving device communicates with the detection cell 310 through a corresponding channel, and the liquid driving device forms negative pressure by sucking gas in the detection cell 310, so that sample liquid is sucked into the detection cell from a sample inlet of the communication detection cell 310, and negative pressure sample introduction is realized. In some embodiments, the sample inlet portion 320 further comprises a sample inlet 321 and a sample channel 322, the sample inlet 321 communicates with the detection cell 310 through the sample channel 322, and the liquid driving device communicates with the detection cell 310 through a mixing channel 329.

Specifically, as shown in fig. 12, the liquid inlet/outlet of the liquid driving device is greater than the liquid storage volume of the detection cell 310, and after the sample inlet 321 is immersed in the sample liquid, the sample liquid can enter the detection cell 310 from the sample channel 322 by the negative pressure provided by the liquid driving device. When the sample liquid in the detection cell 310 reaches a predetermined amount, the sample injection is stopped, the liquid driving device continuously provides negative pressure, so that a part of the sample liquid enters the mixing channel 329, a predetermined amount of positive pressure and negative pressure are periodically provided, the sample liquid flows back and forth between the mixing channel 329 and the detection cell 310, the sample liquid repeatedly enters and exits the detection cell 310, and the liquid flow generated by the sample liquid entering and exiting the detection cell 310 can promote the pre-embedded markers in the detection unit 300 to be mixed with the sample liquid.

In some embodiments, the liquid drive device is preferably a plunger pump. In some embodiments, further, the plunger pump comprises a plunger 325 and a plunger cavity disposed within the substrate, the plunger 325 extending out of the substrate at one end, the plunger cavity communicating with the detection cell 310 through a mixing channel 329.

In some embodiments, a resilient divider 327 is disposed in the plunger cavity, and the divider 327 divides the plunger cavity into a first plunger cavity 326a and a second plunger cavity 326 b. Further, the first plunger cavity 326a is communicated with the detection cell 310 through the mixing channel 329, and the liquid inlet amount of the first plunger cavity 326a is greater than or equal to the liquid storage amount of the detection cell 310. Specifically, in the negative pressure sampling process, when the plunger head contacts the partition 327 and experiences resistance, the sampling can be stopped; the plunger 325 is continuously pumped to make the head of the plunger enter the second plunger cavity 326b, and the plunger 325 reciprocates in the second plunger cavity 326b, so that the sample liquid can flow back and forth between the detection cell 310 and the first plunger cavity 326a, and the purpose of fully mixing the sample liquid and the marker is achieved.

The particle detection device can also adopt a positive pressure sample introduction mode. Specifically, the liquid driving device communicates with the detection cell 310 through a corresponding channel, and the liquid driving device directly applies positive pressure to the sample liquid to guide the sample liquid into the detection cell, so as to realize positive pressure sample introduction. In some embodiments, as shown in fig. 13, the sample injection part 320 further includes a sample injection port 321 and a sample injection channel 322, the liquid driving device communicates with the detection cell 310 through the mixing channel 329, and the sample injection port 321 communicates with the detection cell 310 through the sample injection channel 322 and the liquid driving device.

In some embodiments, the liquid drive device is preferably a plunger pump. In some embodiments, further, the plunger pump includes a plunger 325 and a plunger cavity 326 disposed within the base plate, the plunger 325 extending out of the base plate at one end, the plunger cavity 326 communicating with the detection cell 310 through a mixing channel 329. In some embodiments, the sample inlet 321 opens on the end of the plunger 325 that extends out of the base plate, the sample channel 322 is disposed within the plunger 325, and the sample channel 322 communicates with the detection cell 310 through the plunger cavity 326. In some embodiments, it is preferable that the sample inlet 321 is provided with a removable sealing plug 328.

In some embodiments, the detection cell 300 further includes an exhaust port 330 in communication with the detection cell 310. The vent 330 may be used to vent air to equalize the pressure of the gas inside and outside the cell 310.

Specifically, as shown in fig. 13, the sample injection device may sequentially pass through the sample inlet 321, the sample injection channel 322, the plunger cavity 326, and the mixing channel 329 to introduce the sample solution into the detection cell 310; after the sample introduction is finished, the sample inlet 321 is blocked, the plunger 325 is pulled to provide liquid driving force, so that the sample liquid flows back and forth between the detection cell 310 and the plunger cavity 326, and the sample liquid repeatedly enters and exits the detection cell 310, so that the sample liquid and the marker are fully mixed.

Other structures of the particle detecting device are similar to the aforementioned particle detecting device, such as a substrate structure, and the details thereof can be found in other parts of the disclosure, and are not repeated herein.

The application also discloses an operation method of the particle detection device, which can be applied to the particle detection device. According to the operation method, the sample introduction part of the particle detection device is hermetically connected with the liquid driving device, so that repeated introduction and export of the sample liquid in the detection pool are quickly realized. The operation method comprises the following steps:

introducing a sample liquid into the detection cell through the sample introduction part;

a liquid driving device which is hermetically connected with the sample introduction part provides liquid driving force, so that at least part of the sample liquid repeatedly enters and exits the detection cell;

and driving the sample liquid to flow into the detection cell again through the auxiliary driving device.

In some embodiments, the liquid driving device drives at least a portion of the sample liquid to repeatedly enter and exit the detection cell at least 1 time, so that at least a portion of the markers pre-embedded in the detection unit is mixed with the sample liquid. Preferably, the marker is pre-embedded in the sample introduction part and/or the detection cell. Specifically, the liquid driving device can alternately provide positive pressure and negative pressure, the positive pressure and the negative pressure provided by the liquid driving device can effectively act on the sample liquid through the sealing connection between the liquid driving device and the sample injection part, so that the sample liquid flows back and forth between the sample injection part and the detection pool, and part or all of the sample liquid repeatedly enters and exits the detection pool for 1 time or more than 1 time, so that the pre-embedded markers and the sample liquid can be quickly, fully and uniformly mixed.

In some embodiments, the driving force output part of the liquid driving device is inserted into and tightly presses the sample injection part, so that the liquid driving device is in sealed connection with the sample injection part. In some embodiments, the liquid driving device is a device with positive and negative pressure control; wherein, the device with positive and negative pressure control is one of a liquid transfer device, an injector and a skin blowing device.

For example, the liquid driving device is a pipette, and the pipette can be hermetically connected with the sample injection part by inserting a tip of the pipette into the sample injection part and compressing the tip; after the liquid transfer device introduces the sample liquid into the detection pool, the control button is repeatedly pressed to provide liquid driving force of alternating positive pressure and negative pressure, so that the sample liquid can repeatedly enter and exit the detection pool, and the aim of quickly and uniformly mixing the sample liquid and the marker is fulfilled.

For example, the liquid driving device is an injector, and the injector can be hermetically connected with the sample injection part by inserting an injection head of the injector into the sample injection part and compressing; after the sample liquid is introduced into the detection pool by the injector, the injector plunger rod is repeatedly pulled to provide liquid driving force of alternating positive pressure and negative pressure, so that the sample liquid can repeatedly enter and exit the detection pool, and the aim of quickly and uniformly mixing the sample liquid and the marker is fulfilled.

In some embodiments, the liquid driving device is fixed on the particle detection device, and the liquid driving device is directly or indirectly connected with the sample injection part in a sealing manner.

The beneficial effects that may be brought by the embodiments of the present application include, but are not limited to: among the prior art, because the depth factor of detection pool leads to that liquid surface tension is big in the detection pool, sample liquid in case get into the detection pool, then hardly derive it or the suction, compare with prior art, the particle detection device of this application makes it can seal fit liquid drive arrangement through the structure setting of appearance portion to allow to pass through the cooperation of liquid drive arrangement and appearance portion to make the repeated business turn over of sample liquid detect the pond, carry out the leading-in of sample liquid and derive fast. In some application scenarios, for example, when the marker is pre-embedded in the particle detection device, the sample injection part and the liquid driving device are in sealing fit to enable the sample liquid to repeatedly enter and exit the detection cell, so that the marker and the sample liquid can be rapidly and uniformly mixed. It is to be noted that different embodiments may produce different advantages, and in different embodiments, any one or combination of the above advantages may be produced, or any other advantages may be obtained.

Having thus described the basic concept, it will be apparent to those skilled in the art that the foregoing detailed disclosure is to be regarded as illustrative only and not as limiting the present specification. Various modifications, improvements and adaptations to the present description may occur to those skilled in the art, although not explicitly described herein. Such modifications, improvements and adaptations are proposed in the present specification and thus fall within the spirit and scope of the exemplary embodiments of the present specification.

Also, the description uses specific words to describe embodiments of the description. Reference throughout this specification to "one embodiment," "an embodiment," and/or "some embodiments" means that a particular feature, structure, or characteristic described in connection with at least one embodiment of the specification is included. Therefore, it is emphasized and should be appreciated that two or more references to "an embodiment" or "one embodiment" or "an alternative embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, some features, structures, or characteristics of one or more embodiments of the specification may be combined as appropriate.

Claims (15)

1. A particle detection device comprising a substrate and at least one detection cell disposed on the substrate, wherein the at least one detection cell comprises:

a detection cell, wherein the detection cell can contain a sample liquid;

the intercommunication the appearance portion of advancing of detection pond, the appearance portion of advancing can with liquid drive arrangement sealing connection, liquid drive arrangement with the cooperation of appearance portion is used for making sample liquid advances out the detection pond.

2. The particle detection device of claim 1, wherein the sample introduction portion comprises a sample introduction channel and a sample introduction port communicating with the detection cell through the sample introduction channel.

3. The particle detection device of claim 2, wherein the inner diameter of the sample channel decreases in a direction from the sample inlet to the detection cell.

4. The particle detection device of claim 3, wherein the inner profile of the sample channel is tapered.

5. The particle detection device of claim 2, wherein the substrate comprises a first plate body and a second plate body; the detection pool is arranged on the second plate body; the sample inlet is arranged on the first plate body; the sampling channel is arranged on the first plate body, or the sampling channel is arranged on the first plate body and the second plate body.

6. The particle detection device of claim 5, wherein an axis of the sample channel forms an angle of 20-60 ° with the first plate body.

7. The particle detecting device of claim 5, wherein the first plate and the second plate are sealed, the detecting cell is disposed between the first plate and the second plate, and the sample inlet channel communicates the detecting cell with the sample inlet.

8. The particle detection device of claim 5, wherein the sample inlet is elliptical or circular in shape in a cross-section parallel to the first plate body.

9. The particle detection apparatus of claim 8, wherein the minor axis of the elliptical cross-section of the sample inlet is 0.5mm to 4mm in length.

10. The particle detection device of claim 5, wherein the sample inlet protrudes from the first plate body.

11. The particle detection device of claim 10, wherein a height of the sample inlet protruding from the first plate body is 0.5mm to 3 mm.

12. The particulate detection apparatus of any one of claims 2-11, wherein the length of the sample channel is greater than 1 mm.

13. The particle detection device of any one of claims 2-11, wherein the sample introduction part further comprises an elastic sealing member provided with a through hole, the elastic sealing member being mounted within the sample introduction channel and/or on the sample introduction port.

14. The particulate detection apparatus of any one of claims 1-11, wherein the detection cell has a depth of less than 2 mm.

15. The microparticle detection device as claimed in any one of claims 1 to 11, wherein a marker is embedded in the sample introduction part and/or the detection cell, and at least part of the marker is mixed into the sample liquid entering and exiting the detection cell by cooperation of the liquid driving device and the sample introduction part.

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