CN216082593U - Mass spectrometric analysis preprocessing device for glycoprotein and glycopeptide - Google Patents
Mass spectrometric analysis preprocessing device for glycoprotein and glycopeptide Download PDFInfo
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- CN216082593U CN216082593U CN202121605958.5U CN202121605958U CN216082593U CN 216082593 U CN216082593 U CN 216082593U CN 202121605958 U CN202121605958 U CN 202121605958U CN 216082593 U CN216082593 U CN 216082593U
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Abstract
The utility model discloses a mass spectrometry pretreatment device for glycoprotein and glycopeptide, which comprises a sample loading module, a cleaning module, an electrospray ionization module and a mass spectrometry detection module, wherein the sample loading module comprises a sample injection pump, a sample four-way valve and a three-dimensional movable sample stage, the cleaning module comprises a cleaning injection pump, a cleaning four-way valve and a cleaning bottle, the electrospray ionization module comprises an ionization four-way valve, an electrospray displacement device, a metal two-way I, a metal two-way II, an integrated spray needle column I and an integrated spray needle column II, and the mass spectrometry detection module comprises a mass spectrometer. The online separation and electrospray ionization of the glycoprotein and the glycopeptide of a batch of samples can be automatically realized, and the identification rate of the glycoprotein and the glycopeptide is improved; and the integrated spray needle column is cooperatively matched, so that the pretreatment and sample loading detection are continuously carried out on the samples, the detection time of the samples in batches is shortened, and the efficiency of the pretreatment of the samples is improved.
Description
Technical Field
The utility model belongs to the technical field of mass spectrum pretreatment, and particularly relates to a mass spectrum analysis pretreatment device for glycoprotein and glycopeptide.
Background
Mass spectrometers have been used in a wide variety of applications involving food, environmental, pharmaceutical, human health and national security. When mass spectrometry is performed, because the components of a sample are complex, the sample is usually subjected to pretreatment and then mass spectrometry sample injection analysis is performed. Sample pretreatment is a very important step in mass spectrometry, and directly influences the result of mass spectrometry.
Glycosylation is one of the most important post-translational modifications of proteins, since protein glycosylation is involved in many biological processes, and identification of protein glycosylation is of great significance for the discovery of tumor markers. The protein glycosylation is mainly analyzed and identified by a mass spectrometer, but because the abundance of the glycoprotein is low and the ionization efficiency is not high, the glycoprotein needs to be separated and enriched before mass spectrum sample injection, so that the identification quantity of the glycoprotein is improved. The integrated spray needle column aiming at the glucoprotein and the glucoprotein can realize the separation and the enrichment of the glucoprotein and the glucoprotein, and the electrospray ionization can be carried out immediately after the on-line separation and the enrichment are finished. After the online separation and ionization of a kind of sample are accomplished, need wash the needle post of integration to be used for the analysis of next sample, if accomplish this process of manual completion will consume great energy, also influence the separation effect of needle post easily in manual operation process, so need develop an automatic preprocessing device to glycoprotein and glycopeptide, realize automatically to realizing online separation, enrichment and the ionization of glycopeptide in the sample, and to the washing of needle post, improve the efficiency of sample preliminary treatment.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects of the prior art and provide a mass spectrometry analysis pretreatment device for glycoprotein and glycopeptide, which can automatically realize online separation, enrichment and ionization of glycopeptide in a sample, flush a spray needle column and improve the pretreatment efficiency of the sample.
The technical scheme adopted by the utility model is as follows:
a pretreatment device for mass spectrometry analysis of glycoprotein and glycopeptide comprises a sample loading module, a cleaning module, an electrospray ionization module and a mass spectrometry detection module, wherein the sample loading module comprises an injection pump for a sample, a four-way valve for the sample and a three-dimensional moving sample table, the cleaning module comprises an injection pump for cleaning, a four-way valve for cleaning and a cleaning bottle, the electrospray ionization module comprises a four-way valve for ionization, an electrospray displacement device, a metal two-way I, a metal two-way II, an integrated spray needle column I and an integrated spray needle column II, the mass spectrometry detection module comprises a mass spectrometer, the injection pump for cleaning is connected with a first interface in the four-way valve for cleaning through a connecting pipe, a second interface in the four-way valve for cleaning is connected with a fourth interface in the four-way valve for ionization through a connecting pipe, a third interface in the four-way valve for ionization is connected with one side port of the metal two-way I through a connecting pipe, the other side mouth of metal two-way I meets with the import of integration spray needle post I, the first interface in syringe pump for the sample passes through connecting pipe and sample four-way valve links to each other, the second interface in the sample four-way valve passes through the connecting pipe and links to each other with the first interface in the four-way valve for the ionization, the second interface in the four-way valve for the ionization passes through the connecting pipe and links to each other with one side mouth of metal two-way II, the other side mouth of metal two-way II meets with the import of integration spray needle post II, the export of integration spray needle post II meets with the mass spectrograph, integration spray needle post I and integration spray needle post II set up on the electrospray displacement device, the third interface in the four-way valve for the washing passes through the connecting pipe and links to each other with the washing bottle, the third interface in the four-way valve for the sample passes through the connecting pipe and links to each other with three-dimensional removal sample platform.
A first interface in the four-way valve for cleaning and a second interface in the four-way valve for cleaning are communicated with each other, and a third interface in the four-way valve for cleaning and a fourth interface in the four-way valve for cleaning are communicated with each other.
The first interface in the sample four-way valve and the second interface in the sample four-way valve of the device are communicated with each other, and the third interface in the sample four-way valve and the fourth interface in the sample four-way valve are communicated with each other.
A first interface in the four-way valve for ionization and a second interface in the four-way valve for ionization are communicated with each other, and a third interface in the four-way valve for ionization and a fourth interface in the four-way valve for ionization are communicated with each other.
The electrospray displacement device of the device comprises a high-voltage power supply, an electrospray high-voltage electrode and a metal spring, wherein the high-voltage power supply is connected with the electrospray high-voltage electrode through a lead, the electrospray high-voltage electrode is respectively connected with a metal two-way I and a metal two-way II through leads, and the metal spring is respectively connected with the electrospray high-voltage electrode, the metal two-way I and the metal two-way II through leads.
The integrated spray needle column I and the integrated spray needle column II of the device can be connected in an exchange position.
The integrated spray needle column I or II of the device is filled with a silicon-based hydrophilic material for online separation and electrospray ionization of glycoprotein and glycopeptide.
The four-way valve for cleaning, the four-way valve for sample and the four-way valve for ionization of the device can be replaced by a six-way valve, an eight-way valve, a ten-way valve or a twelve-way valve.
The utility model has the following beneficial effects: the online separation and electrospray ionization of the glycoprotein and the glycopeptide of a batch of samples can be automatically realized, and the identification rate of the glycoprotein and the glycopeptide is improved; and the integrated spray needle column is cooperatively matched, so that the pretreatment and sample loading detection are continuously carried out on the samples, the detection time of the samples in batches is shortened, and the efficiency of the pretreatment of the samples is improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of an electrospray displacement device according to the present invention;
in figure 1, a syringe pump for cleaning; 2. a syringe pump for the sample; 3. a four-way valve for cleaning; 4. cleaning the bottle; 5. a four-way valve for a sample; 6. moving the sample stage in three dimensions; 7. a four-way valve for ionization; 8. an electrospray displacement device; 9. a metal two-way tube I; 91. II, metal two-way connection; 10. an integrated spray needle column I; 101. an integrated spray needle column II; 11. a mass spectrometer; 12. a high voltage power supply; 13. an electrospray high-voltage electrode; 14. a metal spring.
Detailed Description
The utility model will be further described with reference to the accompanying drawings in which:
as shown in fig. 1-2, the present invention comprises a sample loading module, a cleaning module, an electrospray ionization module and a mass spectrometry detection module, wherein the sample loading module comprises a sample injection pump 2 for pushing and absorbing a sample, a sample four-way valve 5 for switching a flow path and a three-dimensional moving sample stage 6 for automatically replacing the sample, the cleaning module comprises a cleaning injection pump 1 for pushing and absorbing a cleaning solution, a cleaning four-way valve 3 for switching the flow path and a cleaning bottle 4 for storing the cleaning solution, the electrospray ionization module comprises an ionization four-way valve 7 for switching the flow path, an electrospray displacement device 8, a metal two-way I9, a metal two-way II 91, an integrated spray needle column I10 and an integrated spray needle column II 101, wherein the electrospray displacement device 8 is used for moving the integrated spray needle column I10 and the integrated spray needle column II 1, the integrated spray needle column I10 and the integrated spray needle column II 1 are used for separating glycoprotein and glycopeptide, the mass spectrometric detection module comprises a mass spectrometer 11 for detecting a sample.
The syringe pump 1 for the washing of this device links to each other through the first interface in connecting pipe and washing cross valve 3, the second interface in washing cross valve 3 links to each other through the fourth interface in connecting pipe and ionization cross valve 7, the third interface in ionization cross valve 7 passes through one side mouth of connecting pipe and two logical I9 of metal, the other side mouth and the integration of two logical I9 of metal spout needle post I10's import and meet, be convenient for wash its inside.
The injection pump 2 for the sample of this device links to each other through the first interface in connecting pipe and the four-way valve 5 for the sample, the second interface in the four-way valve 5 for the sample passes through the connecting pipe and links to each other with the first interface in the four-way valve 7 for ionization, the second interface in the four-way valve 7 for ionization passes through the connecting pipe and links to each other with one side mouth of two logical II 91 of metal, the other side mouth of two logical II 91 of metal meets with the import of II 101 of integration spray needle post, the export of II 101 of integration spray needle post meets with mass spectrometer 11 for carry out online separation and electrospray ionization to glycoprotein and glycopeptide.
The integrated spray needle column I10 and the integrated spray needle column II 101 of the device are arranged on the electrospray displacement device 8, so that the integrated spray needle column I10 and the integrated spray needle column II 101 can be conveniently moved; the integrated spray needle column I and the integrated spray needle column II can be connected in an exchange position according to detection and cleaning conditions, wherein the integrated spray needle column I or the integrated spray needle column II is filled with a silicon-based hydrophilic material for online separation and electrospray ionization of glycoprotein and glycopeptide. The electrospray displacement device 8 comprises a high-voltage power supply 12, an electrospray high-voltage electrode 13 and a metal spring 14, wherein the high-voltage power supply 12 is connected with the electrospray high-voltage electrode 13 through a lead, the electrospray high-voltage electrode 13 is respectively connected with a metal two-way I9 and a metal two-way II 91 through leads, and the metal spring 14 is respectively connected with the electrospray high-voltage electrode 13, the metal two-way I9 and the metal two-way II 91 through leads; when the electrospray displacement device 8 moves, the integrated spray needle column II 101 moves to and is connected with the sample inlet of the mass spectrometer 11, at this time, the metal spring 14 is conducted with the electrospray high-voltage electrode 13, and the high voltage of the high-voltage power supply 12 is transmitted to the metal two-way 9.
A third interface in the four-way valve 3 for cleaning of the device is connected with a cleaning bottle 4 through a connecting pipe, the four-way valve 3 for cleaning switches a flow path, so that the injection pump 1 for cleaning can absorb cleaning liquid from the cleaning bottle 4, after the cleaning liquid is absorbed, the four-way valve 3 for cleaning switches the flow path again, and the injection pump 1 for cleaning can push the cleaning liquid to the four-way valve 7 for ionization. The third interface in the sample four-way valve 5 is connected with a three-dimensional moving sample stage 6 through a connecting pipe, the three-dimensional moving sample stage 6 is provided with a position where a sample to be analyzed is moved, the flow path of the sample is switched by the sample four-way valve 5, so that the sample injection pump 2 can suck the sample, and after the sample is sucked, the flow path of the sample is switched again by the sample four-way valve 5, so that the sample injection pump 2 can push the sample to the ionization four-way valve 7.
A first interface in a four-way valve 3 for cleaning and a second interface in the four-way valve 3 for cleaning of the device are communicated with each other, and a third interface in the four-way valve 3 for cleaning and a fourth interface in the four-way valve 3 for cleaning are communicated with each other; a first interface in the four-way valve 5 for the sample and a second interface in the four-way valve 5 for the sample are communicated with each other, and a third interface in the four-way valve 5 for the sample and a fourth interface in the four-way valve 5 for the sample are communicated with each other; the first port in the four-way valve for ionization 7 and the second port in the four-way valve for ionization 7 are communicated with each other, and the third port in the four-way valve for ionization 7 and the fourth port in the four-way valve for ionization 7 are communicated with each other. Wherein the four-way valve 3 for washing, the four-way valve 5 for sample, and the four-way valve 7 for ionization can be replaced with a six-way valve, an eight-way valve, a ten-way valve, or a twelve-way valve.
The using process of the utility model is as follows:
when online separation and electrospray ionization of glycoprotein and glycopeptide of a batch of samples are carried out, firstly, a three-dimensional moving sample table 6 is provided with a sample and moves to the position of a sample to be analyzed, the flow path of the sample is switched by a four-way valve 5, so that the sample can be sucked by an injection pump 2 through a connecting pipe, after the sample is sucked, the flow path of the sample is switched again by the four-way valve 5, and the sample can be pushed into a four-way valve 7 for ionization along a first interface and a second interface in the communicated sample four-way valve 5 by the injection pump 2. And then the electrospray displacement device 8 moves to enable the integrated spray needle column II 101 to move to a sample inlet of a mass spectrometer 11 and be communicated with the sample inlet, at the moment, a metal spring 14 in the electrospray displacement device 8 is conducted with an electrospray high-voltage electrode 13, and the high voltage of a high-voltage power supply 12 is transmitted to a metal two-way 9. Then, the four-way valve 7 for ionization switches the flow path, and the sample is transferred to the integrated needle column II 101 connected to the mass spectrometer 11 for on-line separation and electrospray ionization.
Meanwhile, the flow path is switched by the four-way valve 3 for cleaning, so that the injection pump 1 for cleaning can absorb the cleaning solution from the cleaning bottle 4, after the cleaning solution is absorbed, the flow path is switched again by the four-way valve 3 for cleaning, so that the injection pump 1 for cleaning can push the cleaning solution to the four-way valve 7 for ionization along the first interface and the second interface in the communicated four-way valve 3 for cleaning, and at the moment, the cleaning solution flows to the integrated spray needle column I10 without sample detection for cleaning. And during the next detection, the two can be connected at the exchange positions according to the detection and cleaning conditions, and the steps are repeated, so that the online separation and the electrospray ionization of the glucoprotein and the glycopeptide of the batch samples are completed.
Other undescribed portions of the present invention are the same as the prior art.
Claims (8)
1. The utility model provides a glycoprotein and glycopeptide mass spectrometry preprocessing device, its characterized in that it includes appearance module, washing module, electrospray ionization module and mass spectrometry detection module, the appearance module of going up includes injection pump (2) for the sample, four-way valve (5) and three-dimensional removal sample platform (6) for the sample, the washing module is including washing with injection pump (1), washing with four-way valve (3) and washing bottle (4), the electrospray ionization module is including ionization with four-way valve (7), electrospray displacement device (8), metal two-way I (9), metal two-way II (91), integrated spray needle post I (10) and integrated spray needle post II (101), the mass spectrometry detection module includes mass spectrometer (11), washing with injection pump (1) through the connecting pipe with wash with first interface in four-way valve (3), the second interface in the washing with four-way valve (3) through the connecting pipe with the third interface in four-way valve (7) for the ionization The four interfaces are connected, a third interface in the four-way valve (7) for ionization is connected with one side interface of a metal two-way valve I (9) through a connecting pipe, the other side interface of the metal two-way valve I (9) is connected with an inlet of an integrated spray needle column I (10), the sample injection pump (2) is connected with a first interface in the four-way valve (5) for sample through a connecting pipe, a second interface in the four-way valve (5) for sample is connected with a first interface in the four-way valve (7) for ionization through a connecting pipe, a second interface in the four-way valve (7) for ionization is connected with one side interface of a metal two-way valve II (91) through a connecting pipe, the other side interface of the metal two-way valve II (91) is connected with an inlet of an integrated spray needle column II (101), an outlet of the integrated spray needle column II (101) is connected with a mass spectrometer (11), the integrated spray needle column I (10) and the integrated spray needle column II (101) are arranged on an electric spray displacement device (8), and a third interface in the four-way valve (3) for cleaning is connected with the cleaning bottle (4) through a connecting pipe, and a third interface in the four-way valve (5) for sample is connected with the three-dimensional moving sample table (6) through a connecting pipe.
2. The pretreatment device for mass spectrometry analysis of glycoprotein and glycopeptide according to claim 1, wherein the first port of the four-way valve for washing (3) and the second port of the four-way valve for washing (3) are connected to each other, and the third port of the four-way valve for washing (3) and the fourth port of the four-way valve for washing (3) are connected to each other.
3. The pretreatment device for mass spectrometry of glycoprotein and glycopeptide according to claim 1, wherein the first port of the four-way valve for sample (5) and the second port of the four-way valve for sample (5) are connected to each other, and the third port of the four-way valve for sample (5) and the fourth port of the four-way valve for sample (5) are connected to each other.
4. The pretreatment apparatus for mass spectrometry of glycoprotein and glycopeptide according to claim 1, wherein the first port of the four-way valve for ionization (7) and the second port of the four-way valve for ionization (7) are connected to each other, and the third port of the four-way valve for ionization (7) and the fourth port of the four-way valve for ionization (7) are connected to each other.
5. The glycoprotein and glycopeptide mass spectrometry pretreatment device according to claim 1, wherein the electrospray displacement device (8) comprises a high-voltage power supply (12), an electrospray high-voltage electrode (13) and a metal spring (14), the high-voltage power supply (12) is connected with the electrospray high-voltage electrode (13) through a lead, the electrospray high-voltage electrode (13) is respectively connected with the metal two-way I (9) and the metal two-way II (91) through leads, and the metal spring (14) is respectively connected with the electrospray high-voltage electrode (13), the metal two-way I (9) and the metal two-way II (91) through leads.
6. The pretreatment device for mass spectrometry of glycoprotein and glycopeptide according to claim 1, wherein the integrated nozzle column I (10) and the integrated nozzle column II (101) are connected in a position-exchangeable manner.
7. The pretreatment device for mass spectrometry of glycoprotein and glycopeptide according to claim 6, wherein the integrated nozzle column I (10) or II (101) is filled with a silicon-based hydrophilic material for on-line separation and electrospray ionization of glycoprotein and glycopeptide.
8. The pretreatment device for mass spectrometry analysis of glycoprotein and glycopeptide according to claim 1, wherein the four-way valve for washing (3), the four-way valve for sample (5) and the four-way valve for ionization (7) can be replaced by a six-way valve, an eight-way valve, a ten-way valve or a twelve-way valve.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121605958.5U CN216082593U (en) | 2021-07-15 | 2021-07-15 | Mass spectrometric analysis preprocessing device for glycoprotein and glycopeptide |
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| CN202121605958.5U CN216082593U (en) | 2021-07-15 | 2021-07-15 | Mass spectrometric analysis preprocessing device for glycoprotein and glycopeptide |
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| CN216082593U true CN216082593U (en) | 2022-03-18 |
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| CN202121605958.5U Active CN216082593U (en) | 2021-07-15 | 2021-07-15 | Mass spectrometric analysis preprocessing device for glycoprotein and glycopeptide |
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