CN216024954U

CN216024954U - Micro-fluidic chip

Info

Publication number: CN216024954U
Application number: CN202122625871.0U
Authority: CN
Inventors: 马勇; 杨翥翔; 王胜昔
Original assignee: Shenzhen Mindray Animal Medical Technology Co Ltd
Current assignee: Shenzhen Mindray Animal Medical Technology Co Ltd
Priority date: 2021-10-29
Filing date: 2021-10-29
Publication date: 2022-03-15
Anticipated expiration: 2031-10-29

Abstract

A micro-fluidic chip is provided with at least one convex mixing piece in a mixing cavity, and when a sample and diluent respectively flow into the mixing cavity from a sample quantitative cavity and a diluent quantitative cavity, the mixing piece blocks the sample and the diluent and divides liquid flow, so that the laminar flow phenomenon of the sample and the diluent is broken, the contact area between flow layers is increased, and the diffusion of the flow layers is facilitated.

Description

Micro-fluidic chip

Technical Field

The application relates to the field of microfluidics, in particular to a microfluidic chip for realizing a microfluidic technology.

Background

Point-of-care testing (POCT) is a subdivision of the field of in vitro diagnostics, and by virtue of its characteristics of small instrument size, rapid testing, and simple operation, it is widely used in the fields of clinical examination, personal health management, disease prevention and monitoring, etc.

The biochemical real-time detection mainly adopts a centrifugal micro-fluidic technology, and a sample cavity, a diluent cavity, a sample quantitative cavity, a diluent quantitative cavity, a sample mixing cavity, a colorimetric cavity, a quality control cavity, a waste liquid cavity and a liquid path micro-channel are integrated on a micro-fluidic chip; the whole flow operation of biochemical detection such as sample quantification, diluent quantification, sample mixing, sample injection, sample detection and the like is realized through the specific centrifugal motion of the microfluidic chip in the biochemical analyzer.

A plurality of colorimetric cavities are designed on the microfluidic chip, and multi-index detection can be realized by pre-packaging different types of freeze-dried reagent balls. In the detection process, the concentration of the diluted sample entering each colorimetric cavity must be accurate and consistent, and the diluted sample is obtained by fully and uniformly mixing the quantified sample and the diluent in the uniformly mixing cavity.

The sample and the diluent are quantified through a sample quantifying cavity and a diluent quantifying cavity on the micro-fluidic chip, and are injected into the uniform mixing cavity through a siphon micro-channel on the micro-fluidic chip after the quantification is finished. The width of a siphon micro-channel of the existing micro-fluidic chip is about 0.1mm, samples and diluent are in laminar flow characteristics when being injected into a mixing cavity, the mixing of the samples and the diluent mainly depends on the diffusion between different flow layers, the mixing speed is related to the contact area of two flow layers, and the mixing time can be very long. The existing centrifugal micro-flow hole biochemical analyzer adopts a mode of repeatedly accelerating and decelerating and rotating a micro-flow control chip, so that liquid flow in a mixing cavity is vibrated, and further turbulent flow is formed. Although the method has an improved mixing speed compared with the free diffusion between the fluid layers, the microfluidic chip also needs to rotate repeatedly for a long time in order to ensure the sufficient mixing, and the rapidity of the inspection is influenced.

Disclosure of Invention

The application provides a new micro-fluidic chip to show a structure that can improve the mixing speed.

Based on above-mentioned purpose, this application provides a micro-fluidic chip in an embodiment, including the chip main part, the chip main part has sample ration chamber, diluent ration chamber, mixing chamber and color comparison chamber, sample ration chamber and diluent ration chamber communicate with mixing chamber respectively, so that the sample in the sample ration intracavity with the diluent in the diluent ration intracavity can flow in mixing is carried out the mixing in the chamber, mixing chamber is equipped with at least one bellied mixing piece to form the effect of cutting apart to the liquid stream, mixing chamber and color comparison chamber intercommunication, so that the mixed liquid in the mixing chamber can flow in the color comparison intracavity.

In one embodiment, the chip main body is provided with a mounting part used for matching with a driving device so that the chip main body can rotate under the driving of the driving device; the kneading member has at least one dividing section which is in contact with the liquid flow and divides the liquid flow.

In one embodiment, the dividing portion is disposed in a forward or reverse direction of the kneading member.

In one embodiment, the dividing part is arranged opposite to the forward rotation or reverse rotation direction of the blending piece.

In one embodiment, the kneading member has at least two segments, at least one segment being disposed in a forward direction of the kneading member and at least one segment being disposed in a reverse direction of the segment.

In one embodiment, at least two segments are arranged away from each other.

In one embodiment, the dividing portion has a cross section in a shape of an outer narrow and an inner wide.

In one embodiment, the dividing portion has an outwardly convex cusp shape.

In one embodiment, the cross section of the blending part is prismatic.

In one embodiment, the blending part is provided with a support and a rotating body, the support is fixedly arranged, the rotating body is rotatably connected to the support, and the rotating body is provided with an outer wall which is concave and convex.

In one embodiment, the rotating body is an impeller, and the rotating axis of the impeller is perpendicular to the bottom wall of the blending cavity.

In one embodiment, the mixing cavity extends in an arc shape, and the circle center corresponding to the arc shape coincides with the rotation center of the chip main body.

In one embodiment, the mixing members are arranged in a plurality of groups along different radial directions of the arc.

In one embodiment, the uniformly mixing parts are arranged in a plurality of groups at different positions along the circumferential direction of the arc.

In one embodiment, the mixing pieces are arranged in the mixing cavity in a staggered manner.

In one embodiment, the blending part is arranged on the bottom wall of the blending cavity.

In one embodiment, the chip main body comprises a base plate and a sealing cover piece, the base plate is provided with a sample quantifying groove, a diluent quantifying groove, a mixing groove and a colorimetric groove, and the sealing cover piece is covered on the sample quantifying groove, the diluent quantifying groove, the mixing groove and the colorimetric groove to form a corresponding sample quantifying cavity, a diluent quantifying cavity, a mixing cavity and a colorimetric cavity.

According to the microfluidic chip of the embodiment, the blending cavity is provided with at least one convex blending part, when the sample and the diluent respectively flow into the blending cavity from the sample quantitative cavity and the diluent quantitative cavity, the blending part blocks the sample and the diluent, divides the liquid flow, breaks the laminar flow phenomenon of the sample and the diluent, increases the contact area between the flow layers, and is beneficial to the diffusion of the flow layers.

Drawings

FIG. 1 shows a partial cell body and a mixing member on a microfluidic chip according to an embodiment of the present disclosure;

FIG. 2 is an enlarged view of a portion of the mixing chamber of the structure shown in FIG. 1;

FIG. 3 shows a structure of a part of a groove and a mixing member on a microfluidic chip according to another embodiment of the present disclosure;

FIG. 4 shows a structure of a part of a groove and a mixing member on a microfluidic chip according to another embodiment of the present disclosure;

FIG. 5 is an enlarged view of a portion of structure a shown in FIG. 4;

FIG. 6 is a schematic view showing the direction of the dividing part and the rotating direction of the kneading member according to an embodiment of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The present embodiment provides a microfluidic chip, which can be applied to biochemical real-time detection. For example, the microfluidic chip may be a centrifugal microfluidic chip, which can perform detection of various biochemical indexes, such as biochemical detection, blood coagulation detection, immunoassay, etc., by using a very small amount of sample by controlling the microfluidic chip to perform a forward and reverse centrifugal motion.

Referring to fig. 1-4, the microfluidic chip includes a chip body 1. Generally, the chip body 1 includes a bottom plate 100 and a cover member, only the bottom plate 100 is shown in the figure, the bottom plate 100 and the cover member may be in an up-down configuration, and the cover member covers the bottom plate 100. For example, the cover may be covered with a film or sheet using pressure sensitive adhesive or ultrasonic welding. Of course, this structure is only an example of the chip body 1, and the chip body 1 may also adopt other possible structures, and the cover is not limited to the film or the thin plate.

The chip main body 1 has a sample quantifying cavity, a diluent quantifying cavity, a mixing cavity, a colorimetric cavity and other related cavity structures, for example, the chassis 100 has a sample quantifying groove 101, a diluent quantifying groove 102, a mixing groove 103 and a colorimetric groove 104, and when the cover sealing member covers the sample quantifying groove 101, the diluent quantifying groove 102, the mixing groove 103 and the colorimetric groove 104, a corresponding sample quantifying cavity, a diluent quantifying cavity, a mixing cavity and a colorimetric cavity are formed.

This sample ration chamber and diluent ration chamber communicate with the mixing chamber respectively to make the sample in the sample ration intracavity and the diluent of diluent ration intracavity can flow into the mixing intracavity and carry out the mixing. The mixing cavity is communicated with the colorimetric cavity so that the mixed liquid in the mixing cavity can flow into the colorimetric cavity. Such as the chambers may communicate via microchannels 105.

Wherein the mixing chamber is provided with at least one raised mixing element 200 for creating a partitioning effect on the fluid flow. After the sample 400 and the diluent 300 respectively flow into the mixing cavity from the sample quantifying cavity and the diluent quantifying cavity, the mixing piece 200 blocks the sample 400 and the diluent 300 and divides the liquid flow, so that the laminar flow phenomenon of the sample 400 and the diluent 300 is broken, the contact area between the laminar flow layers is increased, and the diffusion of the laminar flow layers is facilitated.

The mixing part 200 is arranged to form a structure capable of scattering and separating laminar flow in the mixing cavity, so that the mixing part 200 can be arranged on each cavity wall or other parts of the mixing cavity to form a protruding structure in the mixing cavity. For example, referring to fig. 1-5, in one embodiment, the homogenizing element 200 is disposed on the bottom wall of the homogenizing chamber. The mixing member 200 may be fixed to the bottom wall, or may be integrally formed with the bottom wall, for example, by the bottom wall protruding upward. In addition, the mixing members 200 may be integrally formed or attached to the side walls of the mixing chamber, or even to the top wall (e.g., lid) of the mixing chamber.

The mixing member 200 is spaced from the wall of the mixing chamber to allow the sample 400 and diluent 300 to flow. When there are a plurality of mixing members 200, a gap may be left between the mixing members 200 to allow the sample 400 and the diluent 300 to flow, especially to facilitate discharging the mixed diluted sample solution.

Further, when the micro-fluidic chip moves, the action between the blending part 200 and the liquid flow can be intensified, the laminar flow is repeatedly divided and scattered, the sample 400 and the diluent 300 can be sufficiently oscillated and blended, and the blending effect is further improved

For example, in one embodiment, the chip body 1 has a mounting portion for cooperating with a driving device so that the chip body 1 can be rotated by the driving device. The mounting portion is a portion where the chip body 1 is mounted on a driving device, for example, in fig. 1 to 4, the chip body 1 has a mounting hole 106 to connect with a driving device of a biochemical analyzer or other analysis instruments, and in addition, the mounting portion may also be a fixing structure such as a snap structure or a screw structure, and even in some embodiments, the mounting portion may also be the self or a local area of the chip body 1, and the analysis instrument fixes the chip body 1 through some fixing structures without providing corresponding fixing structures on the chip body 1. After the chip main body 1 is connected with the driving device, the chip main body can move under the driving of the driving device, for example, in the centrifugal microfluidic technology, the driving device controls the microfluidic chip to rotate to form centrifugal motion, and the flow and the uniform mixing of liquid flow are realized through the centrifugal motion. Of course, in some other embodiments, the microfluidic chip may be driven to perform other motions, not limited to rotation, such as linear reciprocation, etc.

In the centrifugal microfluidic technology, when the mixing part 200 is combined with centrifugal motion, the microfluidic chip alternately rotates forwards (clockwise in a top view state) and reversely (anticlockwise in a top view state), and laminar flow is repeatedly divided and broken up in repeated acceleration and deceleration rotation; meanwhile, the liquid flow in the mixing cavity caused by the rotation of the chip is vibrated to form turbulent flow; under the effect of the recombination, the diffusion speed between the sample 400 and the diluent 300 flowing layers is obviously improved.

Referring to fig. 2, the mixing member 200 has at least one dividing portion 201 contacting and dividing the liquid flow, and the dividing portion 201 may be configured in any feasible shape and structure, so long as it can divide and break the laminar flow formed between the sample 400 and the diluent 300, and can more or less promote the mixing of the sample 400 and the diluent 300.

The orientation of the dividing portion 201 may also affect the breaking and dividing effects on the laminar flow to some extent, for example, in some embodiments, when the mixing member 200 rotates forward or backward along with the whole microfluidic chip, the dividing portion 201 is disposed toward the forward or backward direction of the mixing member 200, so that when the liquid flow rotates along with the microfluidic chip, the mixing member 200 can break and divide the liquid flow more easily, and the mixing of the sample 400 and the diluent 300 is promoted. The term "the split part 201 is provided in the normal or reverse rotation direction of the kneading material 200" means that the split part 201 is oriented at an angle (including 0 °) of less than 90 ° with respect to the normal or reverse rotation direction of the kneading material 200, and referring to fig. 6, the angle is an angle C between the direction B1 of the split part 201 and a tangent B2 made in the direction opposite to the rotation direction a of the kneading material 200 from the position of the split part 201.

In the case where the split part 201 is provided to face the kneading member 200 in the normal or reverse rotation direction as shown in fig. 1 to 3, B1 and B2 overlap each other and the angle C is 0 ° as shown in fig. 6. In this case, the dividing portion 201 can contact and divide the liquid flow more directly.

When the microfluidic chip alternately performs forward rotation and backward rotation, referring to fig. 1 to 4, the mixing member 200 has at least two dividing portions 201, wherein at least one dividing portion 201 is disposed toward the forward rotation direction of the mixing member 200, and at least one mixing member 200 is disposed toward the backward rotation direction of the dividing portion 201. That is, when the microfluidic chip rotates forwards or backwards, at least one dividing part 201 can be ensured to form a better dividing angle with the liquid flow, and the uniform mixing effect is improved. The two segments 201 in different directions may be, but are not limited to, arranged exactly opposite (at 180 °).

As shown in fig. 2, in one embodiment, at least two segments 201 of the mixing member 200 are disposed away from each other, i.e., arranged in a manner of completely opposite directions (180 °).

Further, in order to better divide the flow, in one embodiment, the dividing portion 201 has a cross section with a shape that is narrow at the outside and wide at the inside. The narrow portion can more easily split the flow of liquid, and the split flow of liquid flows to the wide portion along the outer wall of the split portion 201. The cross section of the said outer narrow and inner wide shape includes but is not limited to trapezoid, triangle, circular arc, and the profile with outer narrow and inner wide. This cross-section refers to a section parallel to the plane shown in fig. 1 and 3. Based on the shape of the cross section, the dividing portion 201 may be, but not limited to, a conical shape, a truncated cone shape, a pyramid shape, a spherical shape, a prismatic shape, various profile shapes, and the like. This shape of narrow interior width not only cuts apart the liquid stream more easily, also is favorable to mixing the discharge of accomplishing back dilution sample solution, plays the effect of drainage, avoids diluting sample solution and assembles and can't discharge on the cutting part 201 as far as possible.

In one embodiment, the dividing portion 201 may be further designed in an outwardly protruding cusp shape, the outer end of which is more easily inserted into the fluid stream, thereby more easily dividing the fluid stream. Meanwhile, the pointed projection shape rarely forms a dead angle, liquid flow is difficult to stay on the surface of the dividing part 201, and the discharge of diluted sample solution after the uniform mixing is more facilitated.

For example, in the embodiment shown in FIG. 2, the homogenizing element 200 is prismatic in cross-section. The blending part 200 is provided with two dividing parts 201 which are completely separated from each other, and each dividing part 201 is provided with a triangular cross section, so that in the forward rotation and reverse rotation processes of the microfluidic chip, the liquid flow can be well divided, the laminar flow phenomenon is broken, turbulent flow is formed, and blending of the sample 400 and the diluent 300 is promoted.

Referring to fig. 4 and 5, in another embodiment, the mixing member 200 has a support base 210 and a rotating body 220. The support 210 is fixedly arranged, and can be specifically and fixedly arranged on each cavity wall of the blending cavity, namely on the bottom wall of the blending cavity in the figure. The rotating body 220 is rotatably connected to the supporting base 210, for example, the supporting base 210 can be a shaft-shaped body or has a supporting shaft, and the rotating body 220 is sleeved on the supporting base 210. The rotating body 220 and the support 210 can be axially positioned to prevent the rotating body 220 from falling off the upper end of the support 210 when rotating, and the rotating body 220 can be blocked from falling off the support 210 from the upper side by the cover of the microfluidic chip.

The rotating body 220 has an outer wall that undulates. When the micro-fluidic chip moves, the sample 400 and the diluent 300 in the mixing cavity also move, and under the impact of the liquid flow, the rotating body 220 can rotate, so that the liquid flow is additionally subjected to a reverse stirring effect, and the stirring effect can also break up laminar flow and generate turbulent flow, so that the mixing is accelerated.

Referring to fig. 3 and 4, in one embodiment, the rotating body 220 is an impeller, and the rotation axis of the rotating body 220 is perpendicular to the bottom wall of the kneading chamber. In addition, the rotation axis of the rotating body 220 may be disposed at a non-perpendicular angle to the bottom wall or other mounting chamber wall.

Further, the microfluidic chip is generally disc-shaped, so referring to fig. 1-4, in some embodiments, the mixing chamber extends in an arc shape, and the center of the arc coincides with the rotation center of the chip main body 1. Of course, in some embodiments, the mixing chamber may have other shapes than arcuate.

The mixing member 200 may be one or more. When there are a plurality of mixing members 200, as shown in fig. 4, the mixing members 200 can be arranged in groups along different radial directions of the arc, each group having one or more mixing members 200. Alternatively, the mixing members 200 may be arranged in a plurality of groups at different positions along the circumferential direction of the arc, each group having one or more mixing members 200. Or as shown in fig. 2, the mixing members 200 are arranged in a plurality of groups along different radial directions of the arc shape and in a plurality of groups along different circumferential positions of the arc shape. In addition, referring to fig. 1, in an embodiment, the mixing member 200 may also be disposed in the mixing chamber.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.

Claims

1. The utility model provides a micro-fluidic chip, a serial communication port, including the chip main part, the chip main part has sample ration chamber, diluent ration chamber, mixing chamber and color comparison chamber, sample ration chamber and diluent ration chamber communicate with mixing chamber respectively, so that the sample in the sample ration intracavity with the diluent in the diluent ration intracavity can flow in mixing carries out the mixing in the chamber, mixing chamber is equipped with at least one bellied mixing spare to form the effect of cutting apart to the liquid stream, mixing chamber and color comparison chamber intercommunication, so that the mixed liquid in the mixing chamber can flow in the color comparison intracavity.

2. The microfluidic chip of claim 1, wherein the chip body has a mounting portion for cooperating with a driving device to enable the chip body to be rotated by the driving device; the kneading member has at least one dividing section which is in contact with the liquid flow and divides the liquid flow.

3. The microfluidic chip according to claim 2, wherein the dividing portion is disposed toward a forward or reverse rotation direction of the mixing member.

4. The microfluidic chip according to claim 3, wherein the dividing portion is disposed opposite to the blending member in a forward or reverse direction.

5. The microfluidic chip according to claim 2, wherein the mixing member has at least two of the partitions, at least one of the partitions is disposed in a forward direction of the mixing member, and at least one of the mixing members is disposed in a reverse direction of the partitions.

6. The microfluidic chip of claim 5, wherein at least two partitions are disposed away from each other.

7. The microfluidic chip according to claim 2, wherein the dividing part has a cross-section in a shape of an outer narrow and an inner wide.

8. The microfluidic chip according to claim 2, wherein the dividing part has a shape of a pointed protrusion protruding outward.

9. The microfluidic chip according to claim 2, wherein the cross-section of the mixing member is prismatic.

10. The microfluidic chip according to claim 2, wherein the mixing member comprises a support and a rotating body, the support is fixedly arranged, the rotating body is rotatably connected to the support, and the rotating body has an outer wall with concave and convex undulations.

11. The microfluidic chip according to claim 10, wherein the rotating body is an impeller, and the rotation axis of the impeller is perpendicular to the bottom wall of the mixing chamber.

12. The microfluidic chip according to any of claims 1 to 11, wherein the mixing chamber extends in an arc shape, and a center of the circle corresponding to the arc shape coincides with a center of rotation of the chip main body, wherein:

the uniformly mixing pieces are arranged into a plurality of groups along different radial directions of the arc;

or the uniformly mixing pieces are arranged into a plurality of groups at different positions along the circumferential direction of the arc;

or, the mixing pieces are arranged in the mixing cavity in a staggered manner.

13. The microfluidic chip according to any one of claims 1 to 11, wherein the mixing member is disposed on a bottom wall of the mixing chamber.

14. The microfluidic chip according to any one of claims 1 to 11, wherein the chip body comprises a base plate and a cover member, the base plate has a sample quantifying groove, a diluent quantifying groove, a mixing groove and a colorimetric groove, and the cover member covers the sample quantifying groove, the diluent quantifying groove, the mixing groove and the colorimetric groove to form a corresponding sample quantifying cavity, a corresponding diluent quantifying cavity, a corresponding mixing cavity and a corresponding colorimetric cavity.