CN215906219U

CN215906219U - Enzymolysis cell separation device

Info

Publication number: CN215906219U
Application number: CN202122321978.6U
Authority: CN
Inventors: 刘虹梅
Original assignee: Yiming Suzhou Cell Biotechnology Co ltd
Current assignee: Yiming Suzhou Cell Biotechnology Co ltd
Priority date: 2021-09-25
Filing date: 2021-09-25
Publication date: 2022-02-25
Anticipated expiration: 2031-09-25

Abstract

The utility model discloses an enzymolysis cell separation device which comprises a working box body, wherein a control button and a temperature regulator are fixedly embedded on the upper surface of the working box body, the temperature regulator is positioned on the right side of the control button, a time controller is fixedly connected to the right side surface of the working box body, a support plate is fixedly connected to the inner bottom wall of the working box body, a single chip microcomputer is fixedly connected to the front side of the support plate, two sliding grooves are formed in the upper surface of the support plate, and sliding blocks are connected to the insides of the two sliding grooves in a sliding mode. This enzymolysis cell separation device, through setting up the heat transfer carrier, the heat transfer carrier can be with the even inside of transferring to placing the cup of heat, can effectively carry out the enzymolysis to the cell, through setting up heat sensor and temperature regulator, can make things convenient for scientific research personnel to the control of temperature, avoids the high temperature or crosses low and lead to the enzyme activity to reduce, through setting up time controller, the required time of grasp decomposition that can be accurate.

Description

Enzymolysis cell separation device

Technical Field

The utility model belongs to the technical field of enzymolysis, and particularly relates to an enzymolysis cell separation device.

Background

An enzymolysis separation method: in the implementation process of animal embryo transplantation technology, the early embryo needs to be separated into single cells, then the single cells are transplanted into enucleated oocytes, and the mode of separating the embryo into the single cells usually adopts mechanical separation and enzymolysis separation, wherein, the enzymolysis separation is widely adopted because of the advantages of mild conditions, high separation efficiency and high activity of the separated cells.

The enzymolysis cell separation device in the prior art has the following problems when in use:

(1) the existing enzymolysis cell separation device needs to control the temperature and the enzymolysis time of an enzymolysis reaction system in the process of enzymolysis cell separation, and the enzyme activity is reduced due to overhigh or overlow temperature of the enzymolysis reaction system.

(2) The prior enzymolysis cell separation device has the defects that the activity of cells is reduced or the cells are damaged due to overlong enzymolysis time, and the effect of embryo transplantation in the later period is influenced, so that the enzymolysis cell separation device is provided for solving the problems.

SUMMERY OF THE UTILITY MODEL

The utility model provides a cell enzymolysis and separation device, which aims to solve the problems that the temperature and enzymolysis time of an enzymolysis reaction system need to be controlled in the process of cell enzymolysis and separation of the existing cell enzymolysis and separation device, the enzyme activity is reduced due to overhigh or overlow temperature of the enzymolysis reaction system, the cell activity is reduced or cells are damaged due to overlong enzymolysis time, and the effect of later-stage embryo transplantation is influenced.

The utility model is realized in such a way that the cell enzymolysis and separation device comprises a working box body, the upper surface of the working box body is fixedly embedded with a control button and a temperature regulator, the temperature regulator is positioned at the right side of the control button, the right side surface of the working box body is fixedly connected with a time controller, a support plate is fixedly connected with the inner bottom wall of the working box body, a singlechip is fixedly connected with the front surface of the support plate, the upper surface of the carrier plate is provided with two sliding grooves, the insides of the two sliding grooves are both connected with sliding blocks in a sliding way, the upper surfaces of the two sliding blocks are fixedly connected with a heat transfer carrier, the front surface of the heat transfer carrier is fixedly connected with a heat sensor, the upper surface of the heat transfer carrier is provided with a placing groove, the heat transfer device is characterized in that a placing cup is arranged in the placing groove, an electric push rod is fixedly connected to the inner wall of the working box body, and the output end of the electric push rod is fixedly connected with the outer surface of the heat transfer carrier.

Preferably, the inside of work box is equipped with the light, the upper surface of light and the interior roof fixed connection of work box, the inner wall fixedly connected with separation glass of work box, separation glass is located electric putter's top.

Preferably, two symmetrical anti-skid plates are arranged below the working box body, and the upper surfaces of the two anti-skid plates are fixedly connected with the bottom surface of the working box body.

Preferably, the right flank of work box has the guard gate through hinge fixed hinge joint, the front of guard gate is fixed to be inlayed and is had the observation window, the left side of observation window is equipped with the doorknob.

Preferably, an indicator light is arranged in front of the working box body and located above the protective door, and the back of the indicator light is fixedly connected with the front of the working box body.

Preferably, the right side of the work box body is provided with a warning board, the warning board is located below the time controller, and the left side face of the warning board is fixedly connected with the right side face of the work box body.

Compared with the prior art, the utility model has the beneficial effects that: this enzymolysis cell separation device, through setting up the heat transfer carrier, the heat transfer carrier can be with the even inside of transferring to placing the cup of heat, can effectively carry out the enzymolysis to the cell, through setting up heat sensor and temperature regulator, can make things convenient for the scientific research personnel to control the temperature, avoid the high temperature or cross low and lead to the enzymatic activity to reduce, through setting up time controller, the required time of grasp decomposition that can be accurate, avoid time overlength or not enough to lead to the decomposition effect not good, through setting up the light, the light can throw light on the inside of work box, make things convenient for the scientific research personnel to observe the inside condition of work box.

Drawings

FIG. 1 is a schematic perspective view of a work box according to the present invention;

FIG. 2 is a cross-sectional view of a front view of the work box of the present invention;

FIG. 3 is a cross-sectional view of a side view of the work box of the present invention;

fig. 4 is a cross-sectional view of a front view of the heat transfer carrier of the present invention.

In the figure: 1. a working box body; 2. a control button; 3. a temperature regulator; 4. a time controller; 5. a warning board; 6. a protective door; 7. an observation window; 8. an anti-skid plate; 9. a door handle; 10. an indicator light; 11. an illuminating lamp; 12. blocking glass; 13. placing the cup; 14. a carrier plate; 15. a chute; 16. a single chip microcomputer; 17. a slider; 18. a heat transfer carrier; 19. a heat sensor; 20. an electric push rod; 21. and (6) placing the groove.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.

In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

Referring to fig. 1-4, an enzymolysis cell separation device includes a working box 1, a control button 2 and a temperature regulator 3 are fixedly embedded on the upper surface of the working box 1, the temperature regulator 3 is located on the right side of the control button 2, a time controller 4 is fixedly connected to the right side surface of the working box 1, a carrier plate 14 is fixedly connected to the inner bottom wall of the working box 1, a single chip microcomputer 16 is fixedly connected to the front surface of the carrier plate 14, two sliding grooves 15 are formed in the upper surface of the carrier plate 14, sliding blocks 17 are slidably connected to the insides of the two sliding grooves 15, a heat transfer carrier 18 is fixedly connected to the upper surfaces of the two sliding blocks 17, a heat sensor 19 is fixedly connected to the front surface of the heat transfer carrier 18, a placement groove 21 is formed in the upper surface of the heat transfer carrier 18, a placement cup 13 is arranged in the placement groove 21, an electric push rod 20 is fixedly connected to the inner wall of the working box 1, and the output end of the electric push rod 20 is fixedly connected to the outer surface of the heat transfer carrier 18.

In the utility model, an illuminating lamp 11 is arranged inside a working box body 1, the upper surface of the illuminating lamp 11 is fixedly connected with the inner top wall of the working box body 1, barrier glass 12 is fixedly connected with the inner wall of the working box body 1, the barrier glass 12 is positioned above an electric push rod 20, the illuminating lamp 11 can illuminate the inside of the working box body 1, two symmetrical anti-skid plates 8 are arranged below the working box body 1, the upper surfaces of the two anti-skid plates 8 are fixedly connected with the bottom surface of the working box body 1, the friction force between a device and the ground can be increased by arranging the anti-skid plates 8 to prevent the device from sliding, a protective door 6 is fixedly hinged on the right side surface of the working box body 1 through a hinge, an observation window 7 is fixedly embedded on the front surface of the protective door 6, a door handle 9 is arranged on the left side of the observation window 7, a worker can clearly see the condition inside the working box body 1 through the observation window 7, an indicating lamp 10 is arranged in front of the working box body 1, indicator 10 is located the top of guard gate 6, the back of indicator 10 and the front fixed connection of work box 1, indicator 10 can report to the police when operating time finishes, the right side of work box 1 is equipped with warning sign 5, warning sign 5 is located time controller 4's below, the left surface of warning sign 5 and the right flank fixed connection of work box 1, warning sign 5 can play the effect of warning to the staff, notice the standard operation.

The working principle of the utility model is as follows: the scientific research personnel open protection door 6, release the outside of work box 1 with heat transfer carrier 18 through controlling electric putter 20, then put into the inside of placing cup 13 with the required reagent of cell and enzymolysis, then will place the inside of placing cup 13 and put into standing groove 21 and remove heat transfer carrier 18 to the inside of work box 1 and close protection door 6 again, carry out accurate control to the temperature on heat transfer carrier 18 surface and the time that the decomposition needs through controlling temperature regulator 3 and time controller 4, after the decomposition time has arrived, pilot lamp 10 carries out the scintillation suggestion, at this moment just can open protection door 6 and will place cup 13 and shift out to the outside of work box 1 through electric putter 20.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides an enzymolysis isolated cell device, includes work box (1), its characterized in that: the upper surface of the work box body (1) is fixedly embedded with a control button (2) and a temperature regulator (3), the temperature regulator (3) is positioned on the right side of the control button (2), the right side surface of the work box body (1) is fixedly connected with a time controller (4), the inner bottom wall of the work box body (1) is fixedly connected with a support plate (14), the front side of the support plate (14) is fixedly connected with a single chip microcomputer (16), the upper surface of the support plate (14) is provided with two sliding grooves (15), the insides of the two sliding grooves (15) are respectively and slidably connected with a sliding block (17), the upper surfaces of the two sliding blocks (17) are respectively and fixedly connected with a heat transfer carrier (18), the front side of the heat transfer carrier (18) is fixedly connected with a heat sensor (19), the upper surface of the heat transfer carrier (18) is provided with a placement groove (21), and a placement cup (13) is arranged inside the placement groove (21), the inner wall of the working box body (1) is fixedly connected with an electric push rod (20), and the output end of the electric push rod (20) is fixedly connected with the outer surface of the heat transfer carrier (18).

2. An enzymatic cell separation apparatus as set forth in claim 1, wherein: the inside of work box (1) is equipped with light (11), the upper surface of light (11) and the interior roof fixed connection of work box (1), the inner wall fixedly connected with separation glass (12) of work box (1), separation glass (12) are located the top of electric putter (20).

3. An enzymatic cell separation apparatus as set forth in claim 1, wherein: two symmetrical anti-skid plates (8) are arranged below the working box body (1), and the upper surfaces of the two anti-skid plates (8) are fixedly connected with the bottom surface of the working box body (1).

4. An enzymatic cell separation apparatus as set forth in claim 1, wherein: the right flank of work box (1) has connected guard gate (6) through hinge fixed hinge, fixed the inlaying in front of guard gate (6) has observation window (7), the left side of observation window (7) is equipped with doorknob (9).

5. An enzymatic cell separation apparatus according to claim 4, further comprising: an indicator light (10) is arranged in front of the working box body (1), the indicator light (10) is located above the protective door (6), and the back of the indicator light (10) is fixedly connected with the front of the working box body (1).

6. An enzymatic cell separation apparatus as set forth in claim 1, wherein: the right side of the work box body (1) is provided with a warning board (5), the warning board (5) is located below the time controller (4), and the left side face of the warning board (5) is fixedly connected with the right side face of the work box body (1).