CN215866300U - Instrument for counting eosinophilic granulocytes in induced sputum - Google Patents

Abstract

The utility model provides an eosinophilic granulocyte counter for induced sputum, which comprises a warm bath device, a reagent pump device, a liquid transfer device, a flow counting pool device, a detection device and a control system, wherein the warm bath device is used for placing a specimen container which is used for bearing the induced sputum and a liquefaction reagent thereof; the reagent pump device comprises a liquid mixing container and a first pump, wherein the first pump is used for pumping a reagent into the liquid mixing container; the liquid transferring device is used for transferring the reagent in the liquid mixing container into the sample container or transferring the sample in the sample container into the liquid mixing container; the liquid carrying device comprises a counting plate and a second pump, the counting plate is communicated with the liquid mixing container, and the second pump is used for pumping a mixture of a sample and a reagent in the liquid mixing container into the counting plate and flowing out of the counting plate; the detection device shoots the image of the counting plate to calculate the number and the proportion of eosinophils; the cell counter can realize automatic detection of the number and the proportion of the induced sputum eosinophilic granulocytes.

Description

Instrument for counting eosinophilic granulocytes in induced sputum

Technical Field

The utility model belongs to the technical field of biomedicine, and particularly relates to a sputum-inducing eosinophilic granulocyte counter.

Background

The induced sputum detection is increasingly paid attention to and utilized as a noninvasive, safe and reliable airway inflammation evaluation method. In diseases such as asthma, chronic bronchitis and interstitial pneumonia, patients have airway inflammatory reactions, and in the past, airway inflammation is generally evaluated by using a fiber bronchoscope for drawing materials, but the inspection is traumatic and difficult to repeat, so that sputum induction is an important method.

Normal sputum cells are mainly phagocytic and neutrophil granulocytes, with eosinophils and lymphocytes less frequent. Eosinophils are a very important inflammatory cell in the body of asthmatics, and eosinophil elevation is a characteristic hallmark of bronchial asthma, eosinophilic bronchitis, cough variant asthma and allergic diseases. If there are more eosinophils in the sputum, it indicates that asthma is poorly controlled.

Generally, the steps for inducing sputum detection are: the method comprises the steps of firstly, inducing sputum excretion by using hypertonic saline atomization, specifically, inhaling salbutamol by a patient 10 minutes before induction, then detecting the maximum expiratory volume of the patient per second (FEV1), and if the maximum expiratory volume of the patient per second is less than 70%, performing natural cough or isotonic saline induction treatment on the patient; if the content is more than 70%, using hypertonic saline water to induce sputum excretion; screening the expectorated sputum, discarding saliva, and leaving qualified sputum specimen; the sputum specimen is liquefied to obtain a cell suspension, and the total number and proportion of eosinophils in a certain volume of suspension are determined by manual counting through microscopic observation.

However, the subsequent liquefaction and cell counting of the sputum specimen are both manual operations, the efficiency of the manual operations is low, and the detection results of different operators have poor consistency and repeatability.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model aims to provide a cell counting instrument, which aims to solve the technical problems of low efficiency, poor consistency of detection results and poor repeatability of manual liquefaction of specimens and cell counting in the prior art.

In order to achieve the purpose, the utility model adopts the technical scheme that: there is provided a cytometer comprising:

the warm bath device is used for placing a specimen container, and the specimen container is used for bearing a specimen;

the reagent pump device comprises a liquid mixing container and a first pump, wherein the first pump is used for pumping a reagent into the liquid mixing container;

a pipetting device for transferring the reagent in the liquid mixing container into the specimen container or transferring the specimen in the specimen container into the liquid mixing container;

the liquid carrying device comprises a counting plate and a second pump, the counting plate is communicated with the liquid mixing container, and the second pump is used for pumping the sample in the liquid mixing container into the counting plate and enabling the sample to flow out of the counting plate;

the detection device is used for shooting the image of the counting plate to calculate the number and the proportion of the eosinophils; and

and the control system is electrically connected with the warm bath device, the reagent pump device, the liquid transfer device, the liquid carrying device and the detection device.

In one embodiment, the warm bath device comprises a rotating disc provided with a plurality of accommodating grooves, a first heating element arranged on the rotating disc and used for heating the sample container, and a first driving element used for driving the rotating disc to rotate, wherein the accommodating grooves are used for accommodating the sample container.

In one embodiment, the reagent pump device comprises at least three first pumps, each first pump being configured to pump a corresponding reagent into the mixing container.

In one embodiment, the reagent pump device further comprises a second heating element arranged on the liquid mixing container and used for heating the liquid mixing container, and a temperature sensor used for detecting the liquid mixing container.

In one embodiment, the reagent pump device further comprises a first light source and a turbidity sensor, the first light source and the turbidity sensor are respectively arranged at two opposite sides of the liquid mixing container, and light emitted by the first light source is received by the turbidity sensor after passing through the liquid mixing container.

In one embodiment, the pipetting device comprises a pipette for pipetting a reagent or a sample, a first displacement mechanism for driving the pipette to switch positions between the mixing container and the sample container of the incubation device, and a second displacement mechanism for driving the pipette up and down.

In one embodiment, the pipette comprises a tubular body, a piston rod having one end inserted into the tubular body, a piston disposed at the end of the piston rod inserted into the tubular body and in sealing engagement with the inner wall of the tubular body, and a second drive member for driving the piston rod to move along the tubular body.

In one embodiment, the detection device comprises a digital microscope arranged above the counting plate and used for shooting the number of the cells of the counting plate and a second light source arranged below the counting plate and used for illuminating the counting plate.

In one embodiment, the detection device further comprises a third displacement mechanism for driving the digital microscope to ascend and descend.

In one embodiment, the liquid carrying device further comprises a fourth displacement mechanism for driving the counting plate to move along a horizontal direction, and the horizontal direction is perpendicular to the lifting direction of the digital microscope.

The cell counter provided by the utility model has the beneficial effects that: compared with the prior art, the cell counter can realize automatic liquefied sputum specimen and eosinophilic granulocyte counting, can avoid human errors, and effectively ensures the consistency and repeatability of detection results.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

FIG. 1 is a schematic perspective view of a cell counter according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of the internal structure of the cell counter according to the embodiment of the present invention;

FIG. 3 is a schematic perspective view of a hot bath apparatus according to an embodiment of the present invention;

FIG. 4 is a schematic perspective view of a reagent pump device according to an embodiment of the present invention;

FIG. 5 is a schematic cross-sectional view of a reagent pump device according to an embodiment of the present invention;

FIG. 6 is a schematic perspective view of a pipetting device according to an embodiment of the present invention;

FIG. 7 is a schematic perspective view of a liquid carrying device and a detection device provided in an embodiment of the present invention;

FIG. 8 is an exploded view of a detecting device according to an embodiment of the present invention;

FIG. 9 is an exploded view of a carrier liquid device according to an embodiment of the present invention;

fig. 10 is a schematic cross-sectional structural view of a counting plate according to an embodiment of the present invention.

Wherein, in the figures, the respective reference numerals:

100-a warm bath device; 110-a turntable; 111-a receiving groove; 120-a first heating element; 130-a first driving member; 140-O-ring; 150-a specimen container; 200-a reagent pump device; 210-a liquid mixing container; 211-a liquid outlet; 220-a first pump; 230-a second heating element; 240-temperature sensor; 250-a first light source; 260-a turbidity sensor; 300-a pipetting device; 310-a pipette; 311-a tube body; 312-a piston rod; 313-a second drive member; 320-a first displacement mechanism; 321-a first support; 322-a first slide rail; 323-first slider; 324-a third driver; 330-a second displacement mechanism; 331-a second support; 332-a second slide rail; 333-a second slider; 334-a fourth drive; 400-liquid carrying means; 410-counting plate; 411-a first cover plate; 412-a second cover plate; 413-flow channel; 420-a second pump; 430-a fourth displacement mechanism; 431-a fourth support; 432-a fourth slide rail; 433-a fourth slider; 434-fixing plate; 435-sixth driving member; 500-a detection device; 510-a digital microscope; 520-a second light source; 530-a third displacement mechanism; 531-a third slide rail; 532-third slider; 533-fixed seat; 534-fifth driving element; 540-a third support; 600-control system.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings, which is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and is therefore not to be construed as limiting the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

Referring to fig. 1 and fig. 2, a cell counter according to an embodiment of the present invention includes a thermal bath apparatus 100, a reagent pump apparatus 200, a liquid transfer apparatus 300, a liquid carrying apparatus 400, a detection apparatus 500, and a control system 600, wherein, as shown in fig. 3, the thermal bath apparatus 100 is used for placing a sample container 150, and the sample container 150 is used for carrying a sample; as shown in fig. 4 and 5, the reagent pump apparatus 200 includes a mixing container 210 and a first pump 220, the first pump 220 being used to pump the reagent into the mixing container 210; the pipetting device 300 is used for transferring the reagent in the mixed liquid container 210 into the specimen container 150 or transferring the specimen in the specimen container 150 into the mixed liquid container 210; as shown in fig. 7 to 9, the carrier liquid device 400 includes a counting plate 410 and a second pump 420, the counting plate 410 is communicated with the mixing container 210, the second pump 420 is used for pumping the sample in the mixing container 210 into the counting plate 410 and flowing out from the counting plate 410, and the detecting device 500 captures an image of the counting plate 410 to calculate the number of cells; the control system 600 is electrically connected to the warming bath device 100, the reagent pump device 200, the pipetting device 300, the carrier device 400 and the detection device 500, so that the warming bath device 100, the reagent pump device 200, the pipetting device 300, the carrier device 400 and the detection device 500 are controlled by the control system 600 to operate in coordination. In this embodiment, the first pump 220 and the second pump 420 may be, but are not limited to, peristaltic pumps.

The cell counter of the embodiment of the utility model has the working principle that:

(1) the operator places the sputum sample in the sample container 150 and then places the sample container 150 in the warm bath apparatus 100;

(2) the first pump 220 pumps the liquefying agent into the mixed liquid container 210, the liquid-moving device 300 transfers the liquefying agent in the mixed liquid container 210 into the specimen container 150, so that the sputum specimen and the liquefying agent are uniformly mixed, and the specimen is obtained by standing culture;

(3) the pipette device 300 transfers the specimen in the specimen container 150 into the liquid mixing container 210, and the first pump 220 pumps the diluent into the liquid mixing container 210 to dilute the specimen;

(4) the first pump 220 pumps a staining agent into the liquid mixing container 210 to stain eosinophils in the diluted specimen;

(5) the second pump 420 pumps the diluted and stained specimen in the liquid mixing container 210 into the counting plate 410 and flows out of the counting plate 410, and the detecting device 500 captures an image of the counting plate 410 to calculate the number of cells;

(6) the detection device 500 transmits the captured image to the control system 600, and the control system 600 calculates the number of cells and calculates the concentration of eosinophils by dilution factor conversion.

The cell counter of the embodiment of the utility model has the beneficial effects that: compared with the prior art, the cell counter provided by the embodiment of the utility model can realize automatic culture of sputum specimens and eosinophil counting, further calculate the proportion of eosinophils, avoid human errors and effectively ensure the consistency and repeatability of detection results.

As an embodiment of the present invention, as shown in fig. 3, the hot bath apparatus 100 includes a rotating plate 110 having a plurality of receiving slots 111, a first heating member 120 disposed on the rotating plate 110 for heating the sample containers 150, and a first driving member 130 for driving the rotating plate 110 to rotate, wherein the receiving slots 111 are used for receiving the sample containers 150. With this structure, after the specimen container 150 is placed in the receiving container 111, the first heating member 120 can heat the specimen container 150, and constant temperature culture can be realized. In this embodiment, the first heating member 120 may be, but is not limited to, a silica gel heating plate, and a heating wire is disposed inside the silica gel heating plate, and the heating wire is powered on to achieve a heating effect; the plurality of receiving slots 111 are circumferentially distributed on the turntable 110, and the first driving member 130 may be, but not limited to, a motor, and drives the turntable 110 to rotate through belt transmission, so as to move the corresponding specimen container 150 to the lower side of the pipetting device 300.

As one embodiment of the present invention, as shown in fig. 3, an O-ring 140 is disposed in the receiving groove 111, the O-ring 140 is used to fix the specimen container 150, and after the specimen container 150 is placed in the receiving groove 111, the O-ring 140 is interference-fitted between the inner wall of the receiving groove 111 and the outer wall of the specimen container 150.

As an embodiment of the present invention, as shown in fig. 4 and 5, the reagent pump apparatus 200 includes at least three first pumps 220, each first pump 220 is used for pumping a corresponding reagent into the liquid mixing container 210, specifically, an inlet of the first pump 220 is communicated with a reagent bottle containing a liquefying agent, and an outlet of the first pump 220 is communicated with the liquid mixing container 210, so that the first pump 220 can pump the liquefying agent into the liquid mixing container 210; the inlet of the second first pump 220 is communicated with the reagent bottle containing the diluent, and the outlet is communicated with the mixing container 210, so that the second first pump 220 can pump the diluent into the mixing container 210; the third first pump 220 has an inlet in communication with the reagent bottle containing the coloring agent and an outlet in communication with the liquid mixing container 210, so that the third first pump 220 can pump the coloring agent into the liquid mixing container 210. In this embodiment, the outlet of the first pump 220 is connected to the inner side of the mixing container 210, and the reagent pumped by the first pump 220 flows from the inner side of the mixing container 210 to the bottom of the mixing container for being drawn by the pipetting device 300.

It is understood that, since the liquefying agent, the diluting solution and the coloring agent are not simultaneously pumped into the mixing container 210, the reagent pump device 200 may be provided with only one first pump 220, and the one first pump 220 may be used for pumping the liquefying agent, the diluting solution and the coloring agent, and the same technical effect can be achieved, and of course, the number of the first pumps 220 in the reagent pump device 200 may be appropriately adjusted according to the selection and specific requirements of the actual situation, and is not limited thereto.

As an embodiment of the present invention, as shown in fig. 4, the reagent pump device 200 further includes a second heating member 230 provided in the liquid mixing container 210 for heating the liquid mixing container 210, and a temperature sensor 240 for detecting the temperature of the liquid mixing container 210. With this structure, the constant temperature setting of the liquid mixing container 210 can be realized to avoid a large deviation of the detection result due to abnormal temperature. In this embodiment, the second heating member 230 may be, but is not limited to, an electric bar, and heating may be achieved by energizing the electric bar.

As an embodiment of the present invention, as shown in fig. 4 and 5, the reagent pump apparatus 200 further includes a first light source 250 and a turbidity sensor 260, the first light source 250 and the turbidity sensor 260 are respectively disposed at two opposite sides of the mixing container 210, and light emitted from the first light source 250 is received by the turbidity sensor 260 after passing through the mixing container 210. Under this structure, can measure the turbidity of the sample in the muddy liquid container 210 through the cooperation of first light source 250 and turbidity sensor 260, adjust the volume of the diluent that adds according to the turbidity, the turbidity meet the requirements until the sample in the muddy liquid container 210 to follow-up sample after the dilution carries out cell count.

As an embodiment of the present invention, as shown in fig. 5, a liquid outlet 211 is disposed at the bottom of the liquid mixing container 210, and the liquid outlet 211 is communicated with the counting plate 410, so as to discharge all the samples in the liquid mixing container 210.

As an embodiment of the present invention, as shown in fig. 6 in combination with fig. 3 and 4, the pipetting device 300 includes a pipette 310 for pipetting a reagent or a sample, a first displacement mechanism 320 for driving the pipette 310 to switch positions between the liquid mixing container 210 and the sample container 150 of the hot bath device 100, and a second displacement mechanism 330 for driving the pipette 310 to ascend and descend. With this structure, the first displacement mechanism 320 can drive the pipette 310 to move above the liquid mixing container 210 or the sample container 150, the second displacement mechanism 330 can drive the pipette 310 to extend into or withdraw from the liquid mixing container 210 or the sample container 150, the pipette 310 can suck or release liquid, and the reagent in the liquid mixing container 210 can be transferred into the sample container 150 or the sample in the sample container 150 can be transferred into the liquid mixing container 210 by the cooperation of the pipette 310, the first displacement mechanism 320 and the second displacement mechanism 330.

As an embodiment of the present invention, as shown in fig. 6, the pipette 310 includes a tube body 311, a piston rod 312 having one end inserted into the tube body 311, a piston (not shown) disposed at one end of the piston rod 312 inserted into the tube body 311 and hermetically engaged with an inner wall of the tube body 311, and a second driving member 313 for driving the piston rod 312 to move along the tube body 311. In this configuration, the second driving unit 313 drives the piston rod 312 and the piston thereon to move along the tube 311, so as to suck or discharge the liquid, thereby transferring the reagent in the mixing container 210 into the sample container 150 or transferring the sample in the sample container 150 into the mixing container 210. In this embodiment, the second driving member 313 may be a motor, and the second driving member 313 may drive the piston rod 312 and the piston thereon to move along the tube 311 through a screw transmission mechanism (i.e., a lead screw and a nut).

As an embodiment of the present invention, as shown in fig. 6, the first displacement mechanism 320 includes a first supporting member 321, a first sliding rail 322 disposed on the first supporting member 321, a first sliding block 323 slidably connected to the first sliding rail 322, and a third driving member 324 for driving the first sliding block 323 to slide along the first sliding rail 322, and the second displacement mechanism 330 and the pipette 310 are both fixed to the first sliding block 323. With this structure, the third driving member 324 can drive the first slider 323 and the second displacement mechanism 330 thereon and the pipette 310 to slide along the first slide rail 322, so that the pipette 310 moves above the mixing container 210 or the sample container 150. In this embodiment, the third driving member 324 may be, but is not limited to, a motor, and the third driving member 324 may be, but is not limited to, a belt to drive the first sliding block 323 to slide along the first sliding rail 322.

As an embodiment of the present invention, as shown in fig. 6, the second displacement mechanism 330 includes a second support 331 disposed on the first slide 323, a second slide rail 332 disposed on the second support 331, a second slide 333 slidably coupled to the second slide rail 332, and a fourth driver 334 for driving the second slide 333 to slide along the second slide rail 332, and the pipette 310 is disposed on the second slide 333. With this structure, the fourth driving member 334 can drive the second slider 333 and the pipette 310 thereon to slide along the second slide rail 332, so that the pipette 310 can extend into or withdraw from the liquid mixing container 210 or the sample container 150. In this embodiment, the fourth driver 334 may be a motor, and the fourth driver 334 may drive, but is not limited to, the second slider 333 and the pipette 310 thereon to slide along the second slide rail 332 via a screw transmission mechanism (i.e., a lead screw and a nut).

As an embodiment of the present invention, as shown in fig. 7 to 9, the detecting device 500 includes a digital microscope 510 disposed above the counting plate 410 and used for photographing the number of cells of the counting plate 410, and a second light source 520 disposed below the counting plate 410 and used for illuminating the counting plate 410. In this configuration, the digital microscope 510 can clearly capture the image of the counting plate 410, and then transmit the image to the control system 600, and the eosinophil concentration can be obtained by processing the image by the control system 600.

As an embodiment of the present invention, as shown in fig. 7 and 8, the detecting apparatus 500 further includes a third displacement mechanism 530 for driving the digital microscope 510 to move up and down, and the third displacement mechanism 530 drives the digital microscope 510 to move up and down, so that the digital microscope 510 can be automatically focused.

As an embodiment of the present invention, as shown in fig. 7 and 8, the detecting device 500 further includes a third supporting member 540, the second light source 520 is disposed on the third supporting member 540, the third displacement mechanism 530 includes a third slide rail 531 disposed on the third supporting member 540, a third slider 532 slidably connected to the third slide rail 531, a fixing seat 533 disposed on the third slider 532, and a fifth driving member 534 for driving the third slider 532 and the fixing seat 533 thereon to slide along the third slide rail 531, and the digital microscope 510 is disposed on the fixing seat 533. With this structure, the fifth driving member 534 can drive the third slider 532 and the fixing base 533 and the digital microscope 510 thereon to slide along the third sliding rail 531. In this embodiment, the fifth driving member 534 can be a motor, and the fifth driving member 534 drives the fixing base 533 and the third slider 532 to slide along the third sliding track 531 through a screw transmission mechanism (i.e., a lead screw and a nut).

As an embodiment of the present invention, as shown in fig. 7 to 9, the carrier liquid device 400 further includes a fourth shifting mechanism 430 for driving the counting plate 410 to move in a direction perpendicular to the lifting direction of the digital microscope 510, in this structure, the fourth shifting mechanism 430 can drive the counting plate 410 to move in the direction perpendicular to the lifting direction of the digital microscope 510, and the digital microscope 510 can take images of different positions of the counting plate 410.

As an embodiment of the present invention, as shown in fig. 9, the fourth moving mechanism 430 includes a fourth supporting member 431 disposed on the third supporting member 540, a fourth sliding rail 432 disposed on the fourth supporting member 431, a fourth sliding block 433 slidably connected to the fourth sliding rail 432, a fixed plate 434 fixed to the fourth sliding block 433, and a sixth driving member 435 for driving the fourth sliding block 433 and the fixed plate 434 thereon to slide along the fourth sliding rail 432, and the counting plate 410 is disposed on the fixed plate 434. With this structure, the sixth driving element 435 can drive the fourth slider 433, and the fixed plate 434 and the counting plate 410 thereon to slide along the fourth sliding rail 432. In this embodiment, the sixth driving member 435 may be a motor, and the fixed plate 434 and the fourth slider 433 are driven by the sixth driving member 435 to slide along the fourth sliding rail 432 through a screw transmission mechanism (i.e., a lead screw and a nut).

As an embodiment of the present invention, as shown in fig. 10, the counting plate 410 includes a first cover plate 411 and a second cover plate 412 which are oppositely disposed, the first cover plate 411 and the second cover plate 412 are both made of transparent material, the first cover plate 411 and the second cover plate 412 are disposed at an interval, such that a flow channel 413 for passing liquid is formed between the first cover plate 411 and the second cover plate 412, a gap of the flow channel 413 only allows a layer of cells to pass through, one end of the flow channel 413 is communicated with the liquid outlet 211 at the bottom of the liquid mixing container 210, and the other end is communicated with the inlet of the second pump 420, so that the second pump 420 can pump the sample in the liquid mixing container 210 into the counting plate 410 and flow out from the counting plate 410.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. An apparatus for inducing sputum eosinophil count, comprising:

the warm bath device is used for placing a specimen container, and the specimen container is used for bearing a specimen;

the reagent pump device comprises a liquid mixing container and a first pump, wherein the first pump is used for pumping a reagent into the liquid mixing container;

a pipetting device for transferring the reagent in the liquid mixing container into the specimen container or transferring the specimen in the specimen container into the liquid mixing container;

the liquid carrying device comprises a counting plate and a second pump, the counting plate is communicated with the liquid mixing container, and the second pump is used for pumping the sample in the liquid mixing container into the counting plate and enabling the sample to flow out of the counting plate;

the detection device is used for shooting the image of the counting plate to calculate the number and the proportion of the eosinophils; and

and the control system is electrically connected with the warm bath device, the reagent pump device, the liquid transfer device, the liquid carrying device and the detection device.

2. The cytometer as described in claim 1, wherein said thermal bath device comprises a rotating disk having a plurality of receiving slots for receiving said sample containers, a first heating element disposed on said rotating disk for heating said sample containers, and a first driving element for driving said rotating disk to rotate.

3. The cytometer of claim 1 wherein said reagent pump means comprises at least three of said first pumps, each of said first pumps being adapted to pump a corresponding reagent into said fluid mixture container.

4. The cytometer of claim 1 wherein the reagent pump means further comprises a second heating element disposed in the fluid mixing vessel for heating the fluid mixing vessel and a temperature sensor for detecting the temperature of the fluid mixing vessel.

5. The cytometer of claim 1 wherein the reagent pump means further comprises a first light source and a turbidity sensor, the first light source and the turbidity sensor being disposed on opposite sides of the fluid mixing container, respectively, wherein light emitted from the first light source is received by the turbidity sensor after passing through the fluid mixing container.

6. The cytometer of claim 1 wherein the pipetting device comprises a pipette for removing a reagent or sample, a first displacement mechanism for driving the pipette to switch positions between the mixing container and the sample container of the incubation device, and a second displacement mechanism for driving the pipette up and down.

7. The cytometer of claim 6 wherein the pipette comprises a tube, a piston rod having one end inserted into the tube, a piston disposed at the end of the piston rod inserted into the tube and in sealing engagement with the inner wall of the tube, and a second drive member for driving the piston rod to move along the tube.

8. The cell counter according to any one of claims 1 to 7, wherein the detecting means comprises a digital microscope disposed above the counter plate for photographing the number of cells of the counter plate and a second light source disposed below the counter plate for illuminating the counter plate.

9. The cytometer of claim 8 wherein the detection device further comprises a third displacement mechanism for driving the digital microscope up and down.

10. The cell counter of claim 9, wherein the carrier means further comprises a fourth displacement mechanism for driving the counting plate to move in a horizontal direction, the horizontal direction being perpendicular to the direction of raising and lowering the digital microscope.

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