CN215828796U - Experimental device for preparing biological methane based on in-situ reservoir condition coal anaerobic fermentation - Google Patents
Experimental device for preparing biological methane based on in-situ reservoir condition coal anaerobic fermentation Download PDFInfo
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Abstract
The experimental device for preparing the biological methane based on the in-situ reservoir condition through the coal anaerobic fermentation comprises a high-pressure gas supply system, a bacterial liquid injection system, an anaerobic fermentation system and a gas desorption system, wherein the high-pressure gas supply system and the bacterial liquid injection system are respectively connected with an inlet of the anaerobic fermentation system through a gas supply pipe and a liquid supply pipe, an outlet of the anaerobic fermentation system is connected with the gas desorption system, and the anaerobic fermentation system is connected with a liquid taking pipe. The method is safe and simple to operate, low in cost and free of pollution, can truly present anaerobic fermentation metabolism and gas production conditions of the biological fracturing fluid in the in-situ reservoir, and provides test basis for increasing coal bed gas by injecting the biological fracturing fluid in the subsequent field.
Description
Technical Field
The utility model belongs to the technical field of engineering combining microbial coal bed gas production enhancement and carbon dioxide recycling, and particularly relates to an experimental device for preparing biological methane based on in-situ reservoir condition coal anaerobic fermentation.
Background
In recent years, coal-bed gas attracts some people's attention as an associated mineral resource of coal and also as a clean and low-carbon unconventional natural gas energy source. The gas heat value of 1 cubic meter of pure coal bed gas is about 40 megajoules, which is equivalent to 1.26 kilograms of standard coal, and is close to that of the conventional natural gas. When the same gas heat value is generated, the emission of carbon dioxide generated by coal combustion is about three times of that of coal bed gas. Because of the occurrence characteristics of high storage and low permeability of the coal bed gas in China, the development condition of the coal bed gas is not ideal, and the commercial development of the coal bed gas is always not advanced. Therefore, an effective coal bed gas yield increasing technology is urgently needed. Suzaibo et al have sublimed and innovated on the basis of the concept of Microbial Enhanced Coal Bed Methane (MECBM), and further propose a new technical idea of coal bed methane bioengineering (CBGB). The technology is characterized in that a strain which is bred, domesticated and improved is injected into an underground coal bed or a ground fermentation gas production mode is adopted, and partial organic components of coal are converted into methane through anaerobic fermentation, so that the double aims of coal bed gas yield increase and carbon emission reduction are achieved, and the technology is an efficient and low-cost carbon negative technology. Microorganisms are the cheapest "labor" in nature and can convert carbon dioxide to methane without interruption, provided there is a suitable environment. Researchers carry out microorganism anaerobic degradation experiments on coal with different coal grades, and the results show that the residual coal after anaerobic degradation has increased pore gaps, enhanced pore connectivity and further developed methane-adsorbing nanopores. Meanwhile, the methane affinity of the residual coal is reduced after the action of the microorganisms, and the desorption of methane in the coal bed is facilitated. The 'gas increasing, permeability increasing, degradation increasing and emission reducing' effects of the CBGB technology are proved in conventional anaerobic fermentation, and researches on microbial anaerobic degradation of coal-to-biomethane are rare under the high-pressure in-situ reservoir condition with existing coal bed gas. The previous engineering experiments at home and abroad generally inject nutrient solution required by anaerobic metabolism of microorganisms into an underground coal seam, and perform anaerobic fermentation under the action of original flora of the coal seam to prepare the biological methane, but the field experiment that the original flora is acclimatized and improved and prepared into biological fracturing fluid containing high-efficiency methanogenic flora and injected into the underground coal seam is rarely reported by really combining the core concept of CBGB.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the defects in the prior art and provides an experimental device for preparing biological methane by coal anaerobic fermentation based on in-situ reservoir conditions.
In order to solve the technical problems, the utility model adopts the following technical scheme: the experimental device for preparing the biological methane based on the in-situ reservoir condition through the coal anaerobic fermentation comprises a high-pressure gas supply system, a bacterial liquid injection system, an anaerobic fermentation system and a gas desorption system, wherein the high-pressure gas supply system and the bacterial liquid injection system are respectively connected with an inlet of the anaerobic fermentation system through a gas supply pipe and a liquid supply pipe, an outlet of the anaerobic fermentation system is connected with the gas desorption system, and the anaerobic fermentation system is connected with a liquid taking pipe.
Anaerobic fermentation system includes the constant temperature incubator, anaerobic fermentation tank, two-position cross valve and temperature pressure table, anaerobic fermentation tank sets up in the constant temperature incubator, anaerobic fermentation tank top is through the lower interface connection of first connecting pipe with two-position cross valve, temperature pressure table passes through the last interface connection of second connecting pipe with two-position cross valve, the left interface of two-position cross valve is connected with high-pressure gas supply system and fungus liquid injection system simultaneously, the right interface and the gaseous desorption headtotail of two-position cross valve, be equipped with the liquid intaking pipe in the anaerobic fermentation tank, be equipped with the liquid intaking valve on the liquid intaking pipe.
The high-pressure gas supply system comprises a methane gas storage tank and a carbon dioxide gas storage tank, a gas outlet of the methane gas storage tank is connected with an inlet of a gas supply pipe through a first gas pipe, a gas outlet of the carbon dioxide gas storage tank is connected with an inlet of the gas supply pipe through a second gas pipe, a first valve is arranged on the first gas pipe, a second valve is arranged on the second gas pipe, a booster pump, a gas flow meter and a third valve are sequentially arranged on the gas supply pipe along the gas flow direction, and an outlet of the gas supply pipe is connected with a left interface of a two-position four-way valve.
The fungus liquid injection system comprises a liquid storage tank and a vacuum-pumping pump, an inlet of a liquid supply pipe is connected with the liquid storage tank, an outlet of the liquid supply pipe is connected with a left interface of the two-position four-way valve, a metering pump, a fourth valve, a liquid flow meter, a one-way valve and a fifth valve are sequentially arranged on the liquid supply pipe along the liquid flow direction, the vacuum-pumping pump is connected onto the liquid supply pipe between the one-way valve and the fifth valve through the vacuum-pumping pipe, and a vacuum valve is arranged on the vacuum-pumping pipe.
The gas desorption system comprises a gas-water separator and a gas desorption instrument, the inlet of the gas-water separator is connected with the right connector of the two-position four-way valve through a gas-liquid conveying pipe, a sixth valve is arranged on the gas-liquid conveying pipe, the gas inlet of the gas desorption instrument is connected with the outlet of the gas-water separator through a gas conveying pipe, and a seventh valve is arranged at the outlet of the gas-water separator.
By adopting the technical scheme, the experimental method comprises the following steps:
(1) selecting a research area, and preparing a biological fracturing fluid;
(2) installing and connecting an experimental device, and injecting gas components of a high-pressure gas supply system and biological fracturing fluid of a bacteria liquid injection system into an anaerobic fermentation system to carry out microbial anaerobic fermentation gas production;
(3) after the gas production is finished, discharging high-pressure gas in the anaerobic fermentation system through a gas desorption system;
(4) and (4) analyzing the differences of the gas-phase product, the solid-phase product, the liquid-phase product and the flora before and after fermentation.
The specific process of the step (1) is as follows: and (3) by combining the geographical position of the research area, finding out the research factors such as the gas components of the coal reservoir in the research area, the pressure and the temperature of the in-situ reservoir, the critical desorption pressure of the reservoir gas, the chemical characteristics of the coal reservoir water and the like, and preparing the biological fracturing fluid rich in the high-efficiency methanogenic flora by using the underground water of the research area and the natural flora subjected to breeding, domestication and improvement.
The specific process of the step (2) is as follows: after the experimental device is connected, adjusting the temperature of the constant-temperature incubator to the reservoir temperature, putting the processed coal sample into an anaerobic fermentation tank, calculating the free volume of the anaerobic fermentation tank, opening a vacuum valve and a fifth valve, rotating a knob of a two-position four-way valve to communicate a right connector with a lower connector, closing other valves, starting a vacuum pumping pump, vacuumizing the inner space of the anaerobic fermentation tank, and closing the vacuum pumping pump, the vacuum valve and the fifth valve after reaching a set vacuum degree;
starting a booster pump, opening a first valve, a second valve and a third valve, adjusting the proportion of injected methane and carbon dioxide through a gas flowmeter, wherein the proportion of methane and carbon dioxide is the same as the gas component of the coal bed gas in the in-situ reservoir, injecting the methane in a methane gas storage tank and the carbon dioxide in a carbon dioxide gas storage tank into an anaerobic fermentation tank through the booster pump, stopping injecting gas after the booster pump is closed until the critical desorption pressure of the in-situ reservoir of the in-situ coal reservoir in the anaerobic fermentation tank, and simultaneously closing the first valve, the second valve and the third valve;
and then opening the fourth valve and the fifth valve, starting the metering pump, injecting the biological fracturing fluid in the liquid storage tank into the anaerobic fermentation system by the metering pump, recording the volume of the injected liquid by the liquid flow meter, monitoring the temperature and the pressure of the injected liquid by the temperature pressure gauge, closing the metering pump when the pressure in the anaerobic fermentation tank reaches the pressure of the in-situ coal reservoir, stopping injecting the liquid, and simultaneously closing the fourth valve and the fifth valve.
The specific process of the step (3) is as follows: after the microbial gas production period is finished, the right connector of the two-position four-way valve is communicated with the lower connector, the sixth valve is opened, high-pressure gas in the anaerobic fermentation system passes through the gas-water separator, saturated sodium bicarbonate solution prepared in advance is filled in the gas desorption instrument, after the high-pressure gas slowly passes through the gas desorption instrument, the gas concentration and the gas volume passing through the gas desorption instrument are recorded, and the volume change and the total volume change of different component gases in the tank before and after anaerobic fermentation are calculated.
The utility model can realize that one set of device can simultaneously simulate the conditions of a plurality of groups of coal bed gas in-situ reservoir layers, and after the anaerobic fermentation tank in the anaerobic fermentation system reaches the reservoir pressure, the gas production research of the microorganism anaerobic fermentation under different in-situ conditions can be carried out for a plurality of times by adjusting the expected gas injection proportion and the temperature and pressure conditions of the coal reservoir layers and replacing the new anaerobic fermentation tank.
High-pressure gas with the same components as the in-situ coal bed gas is injected into the anaerobic fermentation system, the contact area of the domesticated bacteria liquid and the coal can be increased under the high-pressure condition, the bioavailability of the coal is improved, and the production of coal bed biomethane is facilitated.
At present, it is generally considered that methane is produced by degrading coal with microorganisms through four stages of hydrolysis, acidification, hydrogen production, acetic acid production and methane production, methanogens can only synthesize methane by using hydrogen, carbon dioxide, acetic acid and the like, and carbon dioxide is injected into an anaerobic fermentation tank to increase substrates of hydrogenotrophic methanogens, so that the production of biomethane is realized, the resource utilization of carbon dioxide is realized, and the purpose of carbon emission reduction is achieved.
In summary, the utility model has the following advantages:
(1) the method is safe and simple to operate, low in cost and free of pollution, can truly present anaerobic fermentation metabolism and gas production conditions of the biological fracturing fluid in the in-situ reservoir, and provides experimental basis for increasing coal bed gas by injecting the biological fracturing fluid in the subsequent field.
(2) Under the action of high pressure in the anaerobic fermentation tank, the contact area between coal and bacterial liquid is increased, the bioavailability of the coal is improved, and the output of coal bed gas is enhanced.
(3) Carbon dioxide is injected into the anaerobic fermentation system, a substrate is provided for hydrogenotrophic methanogens in the biological fracturing fluid, and biological methane is synthesized, so that the dual purposes of carbon emission reduction and carbon dioxide resource are fulfilled, and the method has great resource and environmental significance.
Drawings
FIG. 1 is a schematic view of the overall structure of an experimental apparatus according to the present invention;
FIG. 2 is a partially enlarged view showing the connection between the anaerobic fermenter and the two-position four-way valve in FIG. 1.
Detailed Description
As shown in fig. 1 and 2, the experimental apparatus for preparing bio-methane by coal anaerobic fermentation based on in-situ reservoir conditions of the present invention comprises a high pressure gas supply system, a bacteria liquid injection system, an anaerobic fermentation system and a gas desorption system, wherein the high pressure gas supply system and the bacteria liquid injection system are respectively connected with an inlet of the anaerobic fermentation system through a gas supply pipe 1 and a liquid supply pipe 2, an outlet of the anaerobic fermentation system is connected with the gas desorption system, and the anaerobic fermentation system is connected with a liquid taking pipe 3.
Anaerobic fermentation system includes constant temperature incubator 4, anaerobic fermentation tank 5, two-position cross valve 6 and temperature pressure table 7, anaerobic fermentation tank 5 sets up in constant temperature incubator 4, 5 tops of anaerobic fermentation tank are connected through the lower interface of first connecting pipe 8 with two-position cross valve 6, temperature pressure table 7 passes through the last interface connection of second connecting pipe 9 with two-position cross valve 6, the left interface of two-position cross valve 6 simultaneously with high-pressure gas supply system and fungus liquid injection system connection, the right interface and the gaseous desorption headtotail of two-position cross valve 6, be equipped with liquid taking pipe 3 in the anaerobic fermentation tank 5, be equipped with liquid taking valve 10 on the liquid taking pipe 3.
The high-pressure gas supply system comprises a methane gas storage tank 11 and a carbon dioxide gas storage tank 12, wherein a gas outlet of the methane gas storage tank 11 is connected with an inlet of a gas supply pipe 1 through a first gas pipe 13, a gas outlet of the carbon dioxide gas storage tank 12 is connected with an inlet of the gas supply pipe 1 through a second gas pipe 14, a first valve 15 is arranged on the first gas pipe 13, a second valve 16 is arranged on the second gas pipe 14, a booster pump 17, a gas flow meter 18 and a third valve 19 are sequentially arranged on the gas supply pipe 1 along the gas flow direction, and an outlet of the gas supply pipe 1 is connected with a left connector of a two-position four-way valve 6.
The fungus liquid injection system comprises a liquid storage tank 20 and a vacuum-pumping pump 21, an inlet of a liquid supply pipe 2 is connected with the liquid storage tank 20, an outlet of the liquid supply pipe 2 is connected with a left connector of a two-position four-way valve 6, a metering pump 22, a fourth valve 23, a liquid flow meter 24, a one-way valve 25 and a fifth valve 26 are sequentially arranged on the liquid supply pipe 2 along the liquid flow direction, the vacuum-pumping pump 21 is connected on the liquid supply pipe 2 between the one-way valve 25 and the fifth valve 26 through a vacuum-pumping pipe 27, and a vacuum valve 28 is arranged on the vacuum-pumping pipe 27.
The gas desorption system comprises a gas-water separator 29 and a gas desorption instrument 30, the inlet of the gas-water separator 29 is connected with the right connector of the two-position four-way valve 6 through a gas-liquid conveying pipe 31, a sixth valve 32 is arranged on the gas-liquid conveying pipe 31, the gas inlet of the gas desorption instrument 30 is connected with the outlet of the gas-water separator 29 through a gas conveying pipe 33, and the outlet of the gas-water separator 29 is provided with a seventh valve 34.
The experimental method comprises the following steps:
(1) selecting a research area, and preparing a biological fracturing fluid;
(2) installing and connecting an experimental device, and injecting gas components of a high-pressure gas supply system and biological fracturing fluid of a bacteria liquid injection system into an anaerobic fermentation system to carry out microbial anaerobic fermentation gas production;
(3) after the gas production is finished, discharging high-pressure gas in the anaerobic fermentation system through a gas desorption system;
(4) and (4) analyzing the differences of the gas-phase product, the solid-phase product, the liquid-phase product and the flora before and after fermentation.
The specific process of the step (1) is as follows: and (3) by combining the geographical position of the research area, finding out the research factors such as the gas components of the coal reservoir in the research area, the pressure and the temperature of the in-situ reservoir, the critical desorption pressure of the reservoir gas, the chemical characteristics of the coal reservoir water and the like, and preparing the biological fracturing fluid rich in the high-efficiency methanogenic flora by using the underground water of the research area and the natural flora subjected to breeding, domestication and improvement.
The specific process of the step (2) is as follows: after the experimental device is connected, the constant temperature incubator 4 is adjusted to the reservoir temperature (35 ℃), the coal sample is dried for 10 hours at 105 ℃, methane gas adsorbed in the coal sample is desorbed, 50 g of the coal sample is added into the anaerobic fermentation tank 5, the free volume of the anaerobic fermentation tank 5 is calculated by using a reference tank prepared in advance, the vacuum valve 28 and the fifth valve 26 are opened, the knob of the two-position four-way valve 6 is rotated to communicate the right interface with the lower interface, other valves are closed, the vacuum pump 21 is started, the internal space of the anaerobic fermentation tank 5 is vacuumized, and the vacuum pump 21, the vacuum valve 28 and the fifth valve 26 are closed after the set vacuum degree is reached;
then starting a booster pump 17, opening a first valve 15, a second valve 16 and a third valve 19, adjusting the ratio of injected methane and carbon dioxide by a gas flow meter 18, wherein the ratio of the methane and the carbon dioxide is the same as the gas composition of the coal bed gas in the in-situ reservoir, and pumping CH in a methane storage tank 11 by the booster pump 174When the pressure reaches 4 MPa, injecting the carbon dioxide in the carbon dioxide gas storage tank 12 into the anaerobic fermentation tank 5, and CO2When the pressure reaches 4.8 MPa, the booster pump 17 is closed to stop injecting the gas, and the first valve 15, the second valve 16 and the third valve 19 are closed at the same time;
then opening a fourth valve 23 and a fifth valve 26, starting a metering pump 22, injecting the biological fracturing fluid in the liquid storage tank 20 into the anaerobic fermentation system by the metering pump 22, recording the volume of the injected liquid by a liquid flow meter 24, monitoring the temperature and the pressure of the injected liquid by a temperature pressure gauge 7, stopping injection when the pressure in the anaerobic fermentation tank 5 reaches the in-situ coal reservoir pressure, namely when the reading of the temperature pressure gauge 7 reaches the reservoir pressure of 8.5 MPa, recording the pumping volume to be 275 mL, closing the metering pump 22, stopping injecting the liquid, simultaneously closing the fourth valve 23 and the fifth valve 26, and then performing anaerobic fermentation for 30 days.
The specific process of the step (3) is as follows: after the gas production period of the microorganisms is finished, the right connector of the two-position four-way valve 6 is communicated with the lower connector, the sixth valve 32 is opened, high-pressure gas in the anaerobic fermentation system passes through the gas-water separator 29, saturated sodium bicarbonate solution prepared in advance is filled in the gas desorption instrument 30, after the high-pressure gas slowly passes through the gas desorption instrument 30, the gas concentration and the gas volume passing through the gas desorption instrument 30 are recorded, and the volume change and the total volume change of different component gases in the tank before and after anaerobic fermentation are calculated.
The method for carrying out specific experiments by adopting the experimental device for preparing the biological methane by the coal-bed methane in-situ reservoir condition through the coal anaerobic fermentation (taking the coal-bed methane reservoir condition found in certain area of Xinjiang as an example) comprises the following steps:
(1) drying the coal sample at 105 ℃ for 10 hours, desorbing methane gas adsorbed in the coal sample, adding 50 g of the coal sample into the anaerobic fermentation tank, calculating the free volume of the anaerobic fermentation tank by using a reference tank prepared in advance, and vacuumizing the anaerobic fermentation tank by using a vacuum pump.
(2) Sequentially injecting high-purity CH into anaerobic fermentation tank4Pumping high-purity CO into the fermentation tank when the pressure reaches 4 MPa and the gas in the anaerobic fermentation tank reaches adsorption balance and the pressure does not change any more2And stopping injecting when the pressure reaches about 4.8 MPa.
(3) And (3) placing the anaerobic fermentation tank in a constant-temperature incubator at 35 ℃, after the adsorption is stable for a plurality of hours, collecting 30 mL of original components in the anaerobic fermentation tank when the pressure gauge indicates that the components do not rise any more, and performing gas component test at the initial stage.
(4) And pumping the prepared biological fracturing fluid into a fermentation tank through a metering pump, stopping injecting when the pressure indicated by a pressure gauge reaches 8.5 MPa of reservoir pressure, recording the pumping volume to be 275 mL, and then performing anaerobic fermentation for 30 days in the behavior period.
(5) After the fermentation is finished, adding a saturated sodium bicarbonate solution into a gas desorption instrument, beginning to desorb the gas in the anaerobic fermentation tank, and comparing the volume difference between injection and desorption.
The utility model can also inject methane and carbon dioxide with different proportions into the anaerobic fermentation tank 5, and find out the gas component proportion with the highest utilization rate of the carbon dioxide by the microorganism by analyzing the influence of different coal bed gas components on the synthesis of methane by the microorganism by utilizing the carbon dioxide, so as to research a new approach for carbon emission reduction.
Summary of the above test results
The present embodiment is not intended to limit the shape, material, structure, etc. of the present invention in any way, and any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (5)
1. Experimental device for prepare biological methane based on in situ reservoir condition coal anaerobic fermentation, its characterized in that: the anaerobic fermentation system comprises a high-pressure gas supply system, a bacterial liquid injection system, an anaerobic fermentation system and a gas desorption system, wherein the high-pressure gas supply system and the bacterial liquid injection system are respectively connected with an inlet of the anaerobic fermentation system through a gas supply pipe and a liquid supply pipe, an outlet of the anaerobic fermentation system is connected with the gas desorption system, and the anaerobic fermentation system is connected with a liquid taking pipe.
2. The experimental facility for preparing biological methane based on in-situ reservoir condition coal anaerobic fermentation according to claim 1, characterized in that: anaerobic fermentation system includes the constant temperature incubator, anaerobic fermentation tank, two-position cross valve and temperature pressure table, anaerobic fermentation tank sets up in the constant temperature incubator, anaerobic fermentation tank top is through the lower interface connection of first connecting pipe with two-position cross valve, temperature pressure table passes through the last interface connection of second connecting pipe with two-position cross valve, the left interface of two-position cross valve is connected with high-pressure gas supply system and fungus liquid injection system simultaneously, the right interface and the gaseous desorption headtotail of two-position cross valve, be equipped with the liquid intaking pipe in the anaerobic fermentation tank, be equipped with the liquid intaking valve on the liquid intaking pipe.
3. The experimental facility for preparing biological methane based on in-situ reservoir condition coal anaerobic fermentation according to claim 2, characterized in that: the high-pressure gas supply system comprises a methane gas storage tank and a carbon dioxide gas storage tank, a gas outlet of the methane gas storage tank is connected with an inlet of a gas supply pipe through a first gas pipe, a gas outlet of the carbon dioxide gas storage tank is connected with an inlet of the gas supply pipe through a second gas pipe, a first valve is arranged on the first gas pipe, a second valve is arranged on the second gas pipe, a booster pump, a gas flow meter and a third valve are sequentially arranged on the gas supply pipe along the gas flow direction, and an outlet of the gas supply pipe is connected with a left interface of a two-position four-way valve.
4. The experimental facility for preparing biological methane based on in-situ reservoir condition coal anaerobic fermentation according to claim 3, characterized in that: the fungus liquid injection system comprises a liquid storage tank and a vacuum-pumping pump, an inlet of a liquid supply pipe is connected with the liquid storage tank, an outlet of the liquid supply pipe is connected with a left interface of the two-position four-way valve, a metering pump, a fourth valve, a liquid flow meter, a one-way valve and a fifth valve are sequentially arranged on the liquid supply pipe along the liquid flow direction, the vacuum-pumping pump is connected onto the liquid supply pipe between the one-way valve and the fifth valve through the vacuum-pumping pipe, and a vacuum valve is arranged on the vacuum-pumping pipe.
5. The experimental facility for preparing biological methane based on in-situ reservoir condition coal anaerobic fermentation according to claim 4, characterized in that: the gas desorption system comprises a gas-water separator and a gas desorption instrument, the inlet of the gas-water separator is connected with the right connector of the two-position four-way valve through a gas-liquid conveying pipe, a sixth valve is arranged on the gas-liquid conveying pipe, the gas inlet of the gas desorption instrument is connected with the outlet of the gas-water separator through a gas conveying pipe, and a seventh valve is arranged at the outlet of the gas-water separator.
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| WO2023016099A1 (en) * | 2021-10-21 | 2023-02-16 | 河南理工大学 | Test device and method for increasing production of coalbed methane by reducing nanobubbles on coal surface |
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