CN107058451B - Method for degrading and converting low-rank coal by using microbial compound inoculant to increase coal bed methane - Google Patents
Method for degrading and converting low-rank coal by using microbial compound inoculant to increase coal bed methane Download PDFInfo
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Abstract
The invention discloses a method for degrading and converting low-rank coal by using a microbial compound inoculant to increase the yield of coal bed methane, which comprises the following steps: enrichment and domestication of strains, preparation of aerobic composite microbial inoculum, preparation of anaerobic composite microbial inoculum, preparation of nutrient solution, collection of coal bed gas, and treatment of gas production coal bed after the coal bed gas is collected. The invention provides a method for injecting an aerobic composite microbial inoculum and an anaerobic composite microbial inoculum into a low-rank coal bed in stages, which aims to alternately realize aerobic and anaerobic environments to accelerate coal dissolution and methane production, thereby realizing high-efficiency clean utilization of the low-rank coal. In addition, the invention also provides a domestication method of the composite microbial inoculum, a culture medium formula and a nutrient solution formula corresponding to different stages, and the extraction rate of the biological coal bed gas is improved.
Description
Technical Field
The invention belongs to the technical field of biological coal bed gas preparation by microorganisms, and particularly relates to a method for degrading and converting low-rank coal by using a microbial compound inoculant to increase the yield of coal bed gas.
Background
The low-rank coal refers to coal with a low degree of coalification, and specifically includes brown coal, long-flame coal, non-caking coal, weakly caking coal and gas coal. The reserve of low-rank coal in China is rich and accounts for 46 percent of the reserve of coal in China, the reserved reserve of proven lignite reaches 1013 hundred million tons, but the low-rank coal is low in heat value, high in water content and oxygen content, easy to weather or spontaneously combust, poor in stability and not suitable for being used as power fuel for a long time, high in additional cost in mining, easy to cause environmental pollution, incapable of mining and causing energy waste, and capable of causing two difficulties in the development of the low-rank coal, so that how to efficiently utilize the low-rank coal is a new problem of energy utilization.
In recent decades, with the verification of secondary biogas theory, microbial communities originally exist in coal seams, coal can be gradually converted into methane from macromolecules to micromolecules, only microorganisms are influenced by the original stratum environment, and the gas yield is low. Especially, since scott puts forward a secondary biogas theory, a large number of efficient gas production engineering bacteria are discovered, and the microorganism yield-increasing coal bed methane enters the visual field of people as a new coal bed methane yield-increasing mode. The coal is evolved from plants, particularly the low-rank coal usually contains a large amount of polycyclic aromatic organic matters with a lignin structure, and the existing research proves that the low-rank coal is more easily degraded by microorganisms, the calorific value of the coal biodegradation conversion product and the calorific value of the raw coal are approximately 94% -97% of the calorific value of the raw coal, and the humic acid in the coal is increased to different degrees, for example, the humic acid in lignite after being processed by microorganisms is increased from 13.6% of the raw coal to 25% -26%. Therefore, the low-rank coal treated by the microorganisms can not only generate clean gas energy, but also the treated coal sample can further become humic acid resources. Therefore, the microbial coal bed gas production is not only a new method for efficiently utilizing low-rank coal, but also a choice for cleaning technology and green development of coal.
Although the research and exploration of the low-rank coal in-situ degradation of coal-dissolving microorganisms to generate the biomethane gas are started in the prior art, the phenomena of no methane generation or low methane generation efficiency exist after the coal-dissolving microorganisms are injected under natural conditions, and the reasons are analyzed: 1. the coal bed environment has large difference with the laboratory condition, and the injected exogenous bacteria are difficult to adapt to the actual coal bed environment. The coal bed is an underground coal bed with different depths, oxygen contents, temperatures, PH values and pressures; 2. in the process of producing methane, functional floras are incomplete, or the number ratio of floras at each stage is disordered, the synergistic effect of the floras is poor, and the methane cannot be continuously produced; 3. the demand of the injected flora on oxygen is different, some flora is aerobic, some flora is anaerobic, some flora is facultative, and the one-step injection of the exogenous flora is inconvenient for oxygen control; 4. the methanogen is the last step of methanogenesis, the quantity and the quality of the methanogen are the key of methanogenesis, while the coal bed indigenous methanogen is strictly anaerobic in the primary environment, the growth is slow, the quantity is small, the activity is low, even if the exogenous bacteria supply substrates required by the methanogen, the gas production rate and the gas production speed are still slow due to the limitation of the quantity and the activity. Therefore, the key problem of efficiently degrading the low-rank coal and converting the low-rank coal into methane is to solve the problem.
Disclosure of Invention
The invention provides a method for degrading and converting low-rank coal by using a microbial compound inoculant to increase the yield of coal bed gas, and although the research and exploration of the coal-dissolving microbes for in-situ degradation of the low-rank coal to generate biological methane gas are started in the prior art, the problems of coal dissolving speed limitation, no methane production or low methane production efficiency exist after the microbes are injected under natural conditions.
The invention provides a method for degrading and converting low-rank coal by using a microbial compound inoculant to increase the yield of coal bed methane, which is characterized by comprising the following steps of:
step 1, preparation of microbial inoculum
Step 1.1, enrichment and acclimatization of strains
Activating phanerochaete chrysosporium, inoculating the activated phanerochaete chrysosporium into a phanerochaete chrysosporium culture medium, carrying out enrichment expanded culture and domestication, and obtaining phanerochaete chrysosporium fermentation liquor after 7-8 generations of domestication;
activating rhodopseudomonas sphaeroides, inoculating the activated rhodopseudomonas sphaeroides into a rhodopseudomonas sphaeroides culture medium, carrying out enrichment, amplification culture and domestication, and obtaining rhodopseudomonas sphaeroides fermentation liquor after 7-8 generations of domestication;
activating Pseudomonas cepacia, inoculating to Pseudomonas cepacia culture medium, performing enrichment amplification culture and acclimatization for 7-8 generations to obtain Pseudomonas cepacia fermentation broth;
step 1.2, preparation of aerobic composite microbial inoculum
Weighing 6-11 parts of phanerochaete chrysosporium fermentation liquid, 4-6 parts of rhodopseudomonas sphaeroides fermentation liquid and 8-12 parts of pseudomonas cepacia fermentation liquid according to the following weight parts, and then uniformly mixing the components to obtain the aerobic composite microbial inoculum, wherein the total number of colonies in the aerobic composite microbial inoculum is 1.0 × 106-1.0×1011cfu/mL；
Step 1.3, preparation of anaerobic composite microbial inoculum
Sampling a biogas fermentation tank, inoculating the biogas fermentation tank into a methanogen culture medium, carrying out enrichment and expanded culture, and obtaining an anaerobic compound microbial inoculum after 7-8 generations of expanded culture;
the total number of bacterial colonies in the anaerobic compound bacteria agent is 1.0 × 106-1.0×1011cfu/mL；
Step 2, preparation of nutrient solution
Adding 40g of waste molasses, 8.5g of potassium dihydrogen phosphate, 22g of dipotassium hydrogen phosphate, 33g of disodium hydrogen phosphate and 5g of ammonium chloride into 1000g of water respectively, and stirring to dissolve the components to obtain a nutrient solution;
step 3, collecting coal bed gas
Simulating a gas production process of injecting microorganisms into low-rank coal by using a triaxial seepage experiment system, placing a standard coal sample into a reaction kettle, setting an axial pressure to be 3-6MPa and a confining pressure to be 2-5MPa, injecting an aerobic composite microbial inoculum into the reaction kettle according to a seepage pressure constant pressure of 3-6MPa, recording an injection amount after the injection is finished, injecting air into the reaction kettle for 8-12min every 3 days, respectively collecting gas and collecting liquid after gas-liquid separation at an outlet is observed, measuring a gas production rate and gas components, injecting an anaerobic composite microbial inoculum into the reaction kettle at a seepage pressure of 3-6MPa when the content of oxygen in the gas is detected to be lower than 1%, recording the injection amount, starting to collect biological coal bed gas when the content of methane is detected to be 30%, stopping collecting the biological coal bed gas when the content of methane is detected to be lower than 5%, and injecting a nutrient solution, wherein the injection amount of the nutrient solution is 40-60% of the total injection amount of the aerobic composite microbial inoculum and the anaerobic composite microbial inoculum When the methane content is detected to reach 30%, the biological coal bed gas is collected again;
when the injected nutrient solution can not enable the methane content in the injection well to reach 5%, circularly injecting the aerobic composite microbial inoculum, the anaerobic composite microbial inoculum and the nutrient solution according to the sequence;
step 4, processing the coal bed gas after the coal bed gas is collected
And (4) recycling the standard coal sample after the gas production in the step (3) is finished.
Preferably, each liter of the Phanerochaete chrysosporium nitrogen-limiting culture medium comprises the following components: 3.0g of coal powder, 2.0g of dextrin, 1.0g of ammonium tartrate, 1.5mg of vitamin B1, 0.5g of Tween 80, 1.5g of benzyl alcohol, 3.0g of monopotassium phosphate and the balance of sterile water, wherein the pH value is 5-6.
Preferably, the culture medium of the rhodopseudomonas sphaeroides comprises the following components per liter: 3.0g of coal powder, 1.0g of yeast extract, 1.0g of ammonium chloride, 0.2g of dipotassium hydrogen phosphate, 0.3g of manganese chloride tetrahydrate, 2.0mg of copper sulfate pentahydrate, 0.5g of sodium chloride, 1.0g of sodium bicarbonate, 2.0g of sodium acetate, 3.0mg of zinc sulfate heptahydrate and the balance of sterile water.
Preferably, the culture medium contains the following components per liter: 3.0g of coal powder, 6.0g of glucose, 2.0g of beef extract, 3.0g of peptone, 5.0g of sodium glutamate, 1.0g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.1g of potassium chloride and the balance of sterile water.
Preferably, the particle size of the pulverized coal is less than or equal to 0.2 mm.
Preferably, the methanogen culture medium comprises 0.4g of dipotassium hydrogen phosphate, 2.0g of magnesium chloride, 0.4g of potassium dihydrogen phosphate, 1.0g of yeast immersion liquid, 1.0g of ammonium chloride, 2.0g of sodium acetate, 0.2g of potassium chloride, 2.0g of sodium chloride, 10m L of trace element solution and the balance of sterile water per liter.
Preferably, the recycling of the standard coal sample after gas production in the step 3 specifically comprises:
analyzing the coal quality of the standard coal sample after the gas production in the step 3 is finished, and if the ash content in the standard coal sample is analyzed to be reduced to be below 10%, the coal seam containing the standard coal sample can be used for mining medium and low ash coal after the bacterial liquid in the coal seam is extracted; and if the humic acid component in the standard coal sample is increased, injecting dilute hydrochloric acid with the volume concentration of 10% into the reaction kettle to dissolve the standard coal sample, and extracting the dissolved standard coal sample liquid to purify and utilize the humic acid.
Compared with the prior art, the invention has the beneficial effects that:
1) the compound microbial agent provided by the invention has a synergistic effect, can accelerate the enrichment of methanogens and shorten the anaerobic gas production time, and particularly has strong degradation gas production capacity on cellulose, humus and various carbon-containing macromolecules in a low-rank coal seam; in addition, the injection method of the compound microbial agent is simple and easy, has the advantages of low investment, environmental protection, cleanness and the like, has long action time, and can effectively prolong the service life of a gas well.
2) The composite microbial agent also has the effect of dissolving coal, and can effectively improve the number of pores of the porous medium-coal and the connectivity of the pores after the coal dissolving and gas producing are finished, so that the pores and cracks are increased, and the permeability is improved more than that of water injection, heat injection or fracturing. The method also increases the content of methane in the coal bed from the source, is not only a good method for efficiently and cleanly mining low-rank coal, but also a good method for increasing the yield of coal bed gas, and can be popularized to waste coal bed gas wells, goafs or tailings.
Drawings
FIG. 1 is a flow chart of the preparation of a complex microbial inoculum according to the invention;
fig. 2 is a diagram illustrating the effect of the present invention.
Detailed Description
In order to make the technical solutions of the present invention better understood and implemented by those skilled in the art, the present invention is further described below with reference to specific embodiments and the accompanying drawings, but the embodiments are not meant to limit the present invention.
The Phanerochaete chrysosporium, Rhodopseudomonas sphaeroides and Pseudomonas cepacia used in the present invention were purchased from China agricultural microorganism culture Collection center, and the experimental methods described in the following examples are all conventional methods unless otherwise specified.
Example 1
A method for degrading and converting low-rank coal by using a microbial compound inoculant to increase the yield of coal bed methane comprises the following steps:
step 1.1, enrichment and acclimatization of strains
Activating phanerochaete chrysosporium, inoculating the activated phanerochaete chrysosporium into a phanerochaete chrysosporium culture medium, carrying out enrichment, amplification culture and domestication, and obtaining phanerochaete chrysosporium fermentation liquor after 8 generations of domestication;
when the phanerochaete chrysosporium is domesticated and cultured, domestication culture conditions are set in a gradient manner, the tolerance of the strain to hypoxia, high pressure and low temperature is gradually improved, and the specific domestication gradient conditions are set in a table 1:
TABLE 1 Phanerochaete chrysosporium acclimation gradient conditions
Activating the rhodopseudomonas sphaeroides, inoculating the activated rhodopseudomonas sphaeroides in a rhodopseudomonas sphaeroides culture medium, and performing enrichment, amplification culture and domestication for 8 generations to obtain a rhodopseudomonas sphaeroides fermentation broth;
activating Pseudomonas cepacia, inoculating to Pseudomonas cepacia culture medium, performing enrichment amplification culture and acclimatization, and performing acclimatization for 8 generations to obtain Pseudomonas cepacia fermentation liquor;
when the rhodopseudomonas sphaeroides and the pseudomonas cepacia are domesticated and cultured, domestication culture conditions are set in a gradient manner, the tolerance of the strain to hypoxia, high pressure and low temperature is gradually improved, and the specific domestication gradient conditions are the same as the domestication culture gradient conditions of phanerochaete chrysosporium;
step 1.2, preparation of aerobic composite microbial inoculum
Weighing the following components in parts by weight: the Phanerochaete chrysosporium is fermented8 parts of liquid, 5 parts of rhodopseudomonas sphaeroides fermentation liquid and 10 parts of pseudomonas cepacia fermentation liquid, then mixing and uniformly stirring the components to obtain the aerobic composite microbial inoculum, wherein the total number of colonies in the aerobic composite microbial inoculum is 1.0 × 1010cfu/mL；
Step 1.3, preparation of anaerobic composite microbial inoculum
Sampling a biogas fermentation tank, inoculating the biogas fermentation tank into a methanogen culture medium, carrying out enrichment and expanded culture, and obtaining an anaerobic compound microbial inoculum after 8 generations of expanded culture;
the total number of bacterial colonies in the anaerobic compound bacteria agent is 1.0 × 1010cfu/mL；
Step 2, preparation of nutrient solution
Adding 40g of waste molasses, 8.5g of potassium dihydrogen phosphate, 22g of dipotassium hydrogen phosphate, 33g of disodium hydrogen phosphate and 5g of ammonium chloride into 1000g of water respectively, and stirring to dissolve the components to obtain a nutrient solution;
step 3, collecting coal bed gas
Simulating a gas production process of injecting microorganisms into lignite by using a triaxial seepage experiment system, wherein the size of a standard coal sample is phi 50mm х 100mm, the mass is 251.2g, the axial pressure and the confining pressure are respectively set to be 5MPa and 4MPa, an aerobic composite microbial inoculum is injected into a reaction kettle according to the osmotic pressure constant pressure of 4MPa, the injection time is 5h, the injection amount is 420g, air is injected once every 3 days for 10min, an injection valve is closed after the injection is finished, gas collection and liquid collection are respectively carried out after gas-liquid separation at an outlet is observed, the gas production amount and gas components are measured, when the content of oxygen in produced gas is detected to be lower than 1%, the anaerobic composite microbial inoculum is injected at the osmotic pressure of 4MPa, the injection amount is 380g, the injection valve is closed after the injection, the content of methane in the produced gas is detected every day within 20 days continuously, when the content of methane is detected to be 30%, biological coal bed gas starts to be collected, the content of, when the methane content is detected to be lower than 5%, stopping collecting the biological coal bed gas, and injecting a nutrient solution, wherein the injection amount of the nutrient solution is 60% of the total injection amount of the aerobic composite microbial inoculum and the anaerobic composite microbial inoculum, and when the methane content is detected to reach 30%, restarting collecting the biological coal bed gas;
when the injected nutrient solution can not enable the methane content in the injection well to reach 5%, circularly injecting the aerobic composite microbial inoculum, the anaerobic composite microbial inoculum and the nutrient solution according to the sequence;
step 4, processing the coal bed gas after the coal bed gas is collected
Analyzing the coal quality of the standard coal sample after the gas production in the step 3, and analyzing that the ash content in the standard coal sample is 8.3 percent, so that the coal seam containing the standard coal sample can be exploited after the bacterial liquid in the coal seam is extracted; and if the humic acid component in the standard coal sample is increased, injecting 10% dilute hydrochloric acid into the reaction kettle to dissolve the standard coal sample, and extracting the dissolved standard coal sample liquid to purify and utilize the humic acid.
Example 2
Step 1, preparation of microbial inoculum
Step 1.1, enrichment and acclimatization of strains
Activating phanerochaete chrysosporium, inoculating the activated phanerochaete chrysosporium into a phanerochaete chrysosporium culture medium, carrying out enrichment expanded culture and domestication, and obtaining phanerochaete chrysosporium fermentation liquor after 7 generations of domestication;
when the phanerochaete chrysosporium is domesticated and cultured, domestication culture conditions are set in a gradient manner, the tolerance of the strain to hypoxia, high pressure and low temperature is gradually improved, and the specific domestication gradient conditions are set as shown in table 2:
TABLE 2 Phanerochaete chrysosporium acclimation gradient conditions
Activating rhodopseudomonas sphaeroides, inoculating the activated rhodopseudomonas sphaeroides into a rhodopseudomonas sphaeroides culture medium, carrying out enrichment, amplification culture and domestication, and obtaining rhodopseudomonas sphaeroides fermentation liquor after 7 generations of domestication;
activating Pseudomonas cepacia, inoculating to Pseudomonas cepacia culture medium, performing enrichment amplification culture and acclimatization, and performing acclimatization for 7 generations to obtain Pseudomonas cepacia fermentation liquor;
when the rhodopseudomonas sphaeroides and the pseudomonas cepacia are domesticated and cultured, domestication culture conditions are set in a gradient manner, the tolerance of the strain to hypoxia, high pressure and low temperature is gradually improved, and the specific domestication gradient conditions are the same as the domestication culture gradient conditions of phanerochaete chrysosporium;
step 1.2, preparation of aerobic composite microbial inoculum
Weighing 11 parts of phanerochaete chrysosporium fermentation liquor, 4 parts of rhodopseudomonas sphaeroides fermentation liquor and 8 parts of pseudomonas cepacia fermentation liquor in parts by weight, and then uniformly mixing the components to obtain the aerobic composite microbial inoculum, wherein the total number of bacterial colonies in the aerobic composite microbial inoculum is 1.0 × 1011cfu/mL；
Step 1.3, preparation of anaerobic composite microbial inoculum
Sampling a biogas fermentation tank, inoculating the biogas fermentation tank into a methanogen culture medium, carrying out enrichment and expanded culture, and obtaining an anaerobic compound microbial inoculum after 7 generations of expanded culture;
the total number of bacterial colonies in the anaerobic compound bacteria agent is 1.0 × 1011cfu/mL；
Step 2, preparation of nutrient solution
Adding 40g of waste molasses, 8.5g of potassium dihydrogen phosphate, 22g of dipotassium hydrogen phosphate, 33g of disodium hydrogen phosphate and 5g of ammonium chloride into 1000g of water respectively, and stirring to dissolve the components to obtain a nutrient solution;
step 3, collecting coal bed gas
Simulating a gas production process of injecting microorganisms into lignite by using a triaxial seepage experiment system, taking a standard coal sample with the size of phi 50mm х 100mm and the mass of 239.3g, setting the axial pressure and the confining pressure to be 6MPa and 5MPa respectively, injecting an aerobic composite microbial inoculum into a reaction kettle according to the osmotic pressure constant pressure of 3MPa, wherein the injection time is 6h and the injection amount is 380g, injecting air for 12min every 3 days later, closing an injection valve after the injection is finished, respectively collecting gas and liquid after gas-liquid separation at an outlet is observed, measuring the gas production amount and gas components, injecting an anaerobic composite microbial inoculum at the osmotic pressure of 3MPa when the oxygen content in the gas is detected to be lower than 1%, wherein the injection amount is 360g, closing the injection valve after the injection, continuously detecting the methane content in the produced gas every 20 days, starting to collect the biogas when the methane content is detected to be 30%, detecting the methane content in the produced gas every day in the collection process, when the methane content is detected to be lower than 5%, stopping collecting the biological coal bed gas, and injecting a nutrient solution, wherein the injection amount of the nutrient solution is 40% of the total injection amount of the aerobic composite microbial inoculum and the anaerobic composite microbial inoculum, and when the methane content is detected to reach 30%, restarting collecting the biological coal bed gas;
when the injected nutrient solution can not enable the methane content in the injection well to reach 5%, circularly injecting the aerobic composite microbial inoculum, the anaerobic composite microbial inoculum and the nutrient solution according to the sequence;
step 4, processing the coal bed gas after the coal bed gas is collected
Analyzing the coal quality of the standard coal sample after the gas production in the step 3, and analyzing that the ash content in the standard coal sample is 7.8%, which indicates that the coal seam containing the standard coal sample can be used for mining low-middle ash coal after the bacterial liquid in the coal seam is extracted; and if the humic acid component in the standard coal sample is increased, injecting 10% dilute hydrochloric acid into the reaction kettle to dissolve the standard coal sample, and extracting the dissolved standard coal sample liquid to purify and utilize the humic acid.
It is to be noted that each liter of Phanerochaete chrysosporium nitrogen-limiting culture medium comprises the following components: 3.0g of coal powder, 2.0g of dextrin, 1.0g of ammonium tartrate, 1.5mg of vitamin B1, 0.5g of Tween 80, 1.5g of benzyl alcohol, 3.0g of monopotassium phosphate and the balance of sterile water, wherein the pH value is 5-6;
the culture medium of the rhodopseudomonas globosa comprises the following components: 3.0g of coal powder, 1.0g of yeast extract, 1.0g of ammonium chloride, 0.2g of dipotassium hydrogen phosphate, 0.3g of manganese chloride tetrahydrate, 2.0mg of copper sulfate pentahydrate, 0.5g of sodium chloride, 1.0g of sodium bicarbonate, 2.0g of sodium acetate, 3.0mg of zinc sulfate heptahydrate and the balance of sterile water;
the culture medium of the pseudomonas cepacia contains the following components per liter: 3.0g of coal powder, 6.0g of glucose, 2.0g of beef extract, 3.0g of peptone, 5.0g of sodium glutamate, 1.0g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.1g of potassium chloride and the balance of sterile water;
the grain diameter of the coal powder is less than or equal to 0.2 mm;
each liter of methanogen culture medium comprises the following components of 0.4g of dipotassium hydrogen phosphate, 2.0g of magnesium chloride, 0.4g of monopotassium phosphate, 1.0g of yeast immersion liquid, 1.0g of ammonium chloride, 2.0g of sodium acetate, 0.2g of potassium chloride, 2.0g of sodium chloride, 10m of L trace element solution and the balance of sterile water.
The methods in the embodiment 1 and the embodiment 2 both utilize the microbial composite inoculant to degrade and convert low-rank coal, and the yield of the coal bed gas is greatly improved. To illustrate the effects of examples 1 and 2, the specific results of comparing the methane production time, the gas production rate and the total gas production rate of the three injection methods are shown in table 3, with the same experimental conditions but without injecting the aerobic microbial complex microbial inoculum and directly injecting the acclimated anaerobic microbial inoculum into the lignite as a reference:
TABLE 3 gas production parameters of degrading and converting low rank coal by microorganism complex microbial inoculum
| Sample (I) | Initial gas production time (d) | Gas production rate (m L/D.kg) | Gas production rate (L) |
| Example 1 | 5 | 103.2 | 2.59 |
| Example 2 | 5 | 80.7 | 1.93 |
| Control sample | 9 | 20.6 | 0.5 |
As can be seen from Table 3, the gas production rate and the gas production rate of the example 1 and the example 2 are far higher than those of the low-rank coal without adding the microbial compound inoculant, so that the method for degrading and converting the low-rank coal by using the microbial compound inoculant to increase the coal bed methane has obvious advantages and can be popularized and used.
Examples 1 and 2, in the first stage, a domesticated aerobic composite microbial agent is injected, and the porosity and permeability of a coal bed are increased by using an anaerobic living environment and a solvent generated by the aerobic composite microbial agent, so that a product after the solvent is further converted by subsequently injected anaerobic microorganisms; then, injecting an anaerobic compound microbial inoculum in the second stage, domesticating the compound microbial inoculum according to actual formation occurrence conditions, wherein the compound microbial inoculum has the main function of efficiently producing methane, and converting carbon dioxide and hydrogen in the first stage into methane under anaerobic conditions; and a third stage of injecting nutrient solution to maintain the activity of the microorganisms and maintain the pH value of the bacterial solution. The three stages have synergistic effect, accelerate the enrichment of methanogen, shorten the anaerobic gas production time, and particularly have strong capability of degrading and producing gas on cellulose, humus and various low-rank coals containing carbon macromolecules in the low-rank coal seam. Therefore, the method increases the content of methane in the coal bed from the source, is not only a good method for efficiently and cleanly mining low-rank coal, but also a good method for increasing the yield of coal bed gas, and can be popularized to waste coal bed gas wells, goafs or tailings.
In the claims of the present invention referring to numerical ranges, it should be understood that both endpoints of each numerical range and any value between the two endpoints can be selected, and since the steps and methods adopted are the same as those of the embodiments 1-2, the preferred embodiments of the present invention have been described for the prevention of redundancy, but once the basic inventive concept is known, those skilled in the art can make other changes and modifications to the embodiments. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, it is intended that such changes and modifications be included within the scope of the appended claims and their equivalents.
Claims (7)
1. A method for degrading and converting low-rank coal by using a microbial compound inoculant to increase the yield of coal bed methane is characterized by comprising the following steps:
step 1, preparation of microbial inoculum
Step 1.1, enrichment and acclimatization of strains
Phanerochaete chrysosporium (A)Phanerochaete chrysosporium) Inoculating the activated strain into a phanerochaete chrysosporium culture medium for enrichment, expansion culture and domestication, and obtaining phanerochaete chrysosporium fermentation liquor after 7-8 generations of domestication;
the specific acclimation gradient conditions of the phanerochaete chrysosporium are set as shown in table 1:
TABLE 1 Phanerochaete chrysosporium acclimation gradient conditions
Pseudomonas globosa (A) and (B)Rhodopseudomonas spheroides) Inoculating the activated pseudomonas sphaerica to a pseudomonas sphaerica culture medium for enrichment, amplification culture and domestication, and obtaining pseudomonas sphaerica fermentation liquor after 7-8 generations of domestication;
pseudomonas cepacia (A)Pseudomonas Cepacia) Inoculating the activated pseudomonas cepacia to a pseudomonas cepacia culture medium for enrichment, amplification culture and domestication, and obtaining pseudomonas cepacia fermentation liquor after 7-8 generations of domestication;
the specific domestication gradient conditions of the rhodopseudomonas sphaeroides and the pseudomonas cepacia are the same as the domestication culture gradient conditions of the phanerochaete chrysosporium;
step 1.2, preparation of aerobic composite microbial inoculum
Weighing the following components in parts by weight: 6-11 parts of phanerochaete chrysosporium fermentation liquor, 4-6 parts of rhodopseudomonas sphaeroides fermentation liquor and 8-12 parts of pseudomonas cepacia fermentation liquor; however, the device is not suitable for use in a kitchenThen, uniformly mixing all the components to obtain the aerobic composite microbial inoculum, wherein the total number of bacterial colonies in the aerobic composite microbial inoculum is 1.0 × 106-1.0×1011cfu/mL；
Step 1.3, preparation of anaerobic composite microbial inoculum
Sampling a biogas fermentation tank, inoculating the biogas fermentation tank into a methanogen culture medium, carrying out enrichment and expanded culture, and obtaining an anaerobic compound microbial inoculum after 7-8 generations of expanded culture;
the total number of bacterial colonies in the anaerobic compound bacteria agent is 1.0 × 106-1.0×1011cfu/mL；
Step 2, preparation of nutrient solution
Adding 40g of waste molasses, 8.5g of potassium dihydrogen phosphate, 22g of dipotassium hydrogen phosphate, 33g of disodium hydrogen phosphate and 5g of ammonium chloride into 1000g of water respectively, and stirring to dissolve the components to obtain a nutrient solution;
step 3, collecting coal bed gas
Simulating a gas production process of injecting microorganisms into low-rank coal by using a triaxial seepage experiment system, placing a standard coal sample into a reaction kettle, setting an axial pressure to be 3-6MPa and a confining pressure to be 2-5MPa, injecting an aerobic composite microbial inoculum into the reaction kettle according to a seepage pressure constant pressure of 3-6MPa, recording an injection amount after the injection is finished, injecting air into the reaction kettle for 8-12min every 3 days, respectively collecting gas and collecting liquid after gas-liquid separation at an outlet is observed, measuring a gas production rate and gas components, injecting an anaerobic composite microbial inoculum into the reaction kettle at a seepage pressure of 3-6MPa when the content of oxygen in the gas is detected to be lower than 1%, recording the injection amount, starting to collect biological coal bed gas when the content of methane is detected to be 30%, stopping collecting the biological coal bed gas when the content of methane is detected to be lower than 5%, and injecting a nutrient solution, wherein the injection amount of the nutrient solution is 40-60% of the total injection amount of the aerobic composite microbial inoculum and the anaerobic composite microbial inoculum When the methane content is detected to reach 30%, the biological coal bed gas is collected again;
when the injected nutrient solution can not enable the methane content in the injection well to reach 5%, circularly injecting the aerobic composite microbial inoculum, the anaerobic composite microbial inoculum and the nutrient solution according to the sequence;
step 4, processing the coal bed gas after the coal bed gas is collected
And (4) recycling the standard coal sample after the gas production in the step (3) is finished.
2. The method for degrading and converting low-rank coal by using the microbial compound inoculant to increase the production of coal bed methane according to claim 1, wherein each liter of the Phanerochaete chrysosporium nitrogen-limiting culture medium comprises the following components: 3.0g of coal powder, 2.0g of dextrin, 1.0g of ammonium tartrate, 1.5mg of vitamin B1, 0.5g of Tween 80, 1.5g of benzyl alcohol, 3.0g of monopotassium phosphate and the balance of sterile water, wherein the pH value is 5-6.
3. The method for degrading and converting low-rank coal by using the microbial compound inoculant to increase the yield of coal bed methane according to claim 1, wherein the culture medium of the pseudomonas sphaerica contains the following components per liter: 3.0g of coal powder, 1.0g of yeast extract, 1.0g of ammonium chloride, 0.2g of dipotassium hydrogen phosphate, 0.3g of manganese chloride tetrahydrate, 2.0mg of copper sulfate pentahydrate, 0.5g of sodium chloride, 1.0g of sodium bicarbonate, 2.0g of sodium acetate, 3.0mg of zinc sulfate heptahydrate and the balance of sterile water.
4. The method for degrading and converting low-rank coal by using the microbial compound inoculant to increase the production of coal bed methane according to claim 1, wherein the pseudomonas cepacia culture medium contains the following components per liter: 3.0g of coal powder, 6.0g of glucose, 2.0g of beef extract, 3.0g of peptone, 5.0g of sodium glutamate, 1.0g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.1g of potassium chloride and the balance of sterile water.
5. The method for degrading and converting low-rank coal to increase the production of coal bed methane by using the microbial composite inoculant according to any one of claims 2 to 4, wherein the particle size of the pulverized coal is less than or equal to 0.2 mm.
6. The method for degrading and converting low-rank coal by using the microbial compound inoculant to increase production of coal bed methane according to claim 1, wherein each liter of the methanogen culture medium comprises 0.4g of dipotassium hydrogen phosphate, 2.0g of magnesium chloride, 0.4g of potassium dihydrogen phosphate, 1.0g of yeast immersion liquid, 1.0g of ammonium chloride, 2.0g of sodium acetate, 0.2g of potassium chloride, 2.0g of sodium chloride, 10m of L trace element solution and the balance of sterile water.
7. The method for degrading and converting low-rank coal by using the microbial compound inoculant to increase the yield of coal bed methane according to claim 1, wherein the recycling of the standard coal sample subjected to gas production in the step 3 specifically comprises the following steps:
analyzing the coal quality of the standard coal sample after the gas production in the step 3 is finished, and if the ash content in the standard coal sample is analyzed to be reduced to be below 10%, the coal seam containing the standard coal sample can be used for mining medium and low ash coal after the bacterial liquid in the coal seam is extracted; and if the humic acid component in the standard coal sample is increased, injecting dilute hydrochloric acid with the volume concentration of 10% into the reaction kettle to dissolve the standard coal sample, and extracting the dissolved standard coal sample liquid to purify and utilize the humic acid.
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| CN107353069A (en) * | 2017-08-29 | 2017-11-17 | 内蒙古科技大学 | A kind of methane/hydrogen coproduction organic fertilizer integration installation for fermenting and fermentation process |
| CN108977245B (en) * | 2018-07-11 | 2020-07-21 | 太原理工大学 | Aerobic and anaerobic microorganism combined treatment system and method for gas in mine goaf |
| CN114634897A (en) * | 2022-04-07 | 2022-06-17 | 内蒙古工业大学 | Method for degrading lignite and microbial inoculum thereof |
| CN114921518A (en) * | 2022-05-23 | 2022-08-19 | 成都能生材科技开发有限责任公司 | Nano-microorganism co-production technology for coal bed adsorbed gas, coal-to-hydrogen gas and coal-to-methane |
| CN114778792B (en) * | 2022-06-24 | 2022-09-23 | 中国煤炭地质总局勘查研究总院 | Biological yield increase experimental system for coal bed gas |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580851A (en) * | 2009-06-19 | 2009-11-18 | 西安科技大学 | Method for directionally transforming low-deterioration coal through photo-bio coupling |
| CN102645396A (en) * | 2012-05-11 | 2012-08-22 | 太原理工大学 | Test method for improving coal rock permeability and device thereof |
| CN102822346A (en) * | 2009-12-18 | 2012-12-12 | 西里斯能源公司 | Biogasification of coal to methane and other useful products |
| CN102900411A (en) * | 2012-10-29 | 2013-01-30 | 河南理工大学 | Biological permeability-increasing method for coal reservoir |
| CN103045652A (en) * | 2012-11-14 | 2013-04-17 | 山西晋城无烟煤矿业集团有限责任公司 | Method for converting brown coal into methane by utilizing microorganism |
| CN104445592A (en) * | 2014-12-23 | 2015-03-25 | 吉林建筑大学 | Method for treating lignite quality-improving waste water through aerobic cometabolism |
| CN104445610A (en) * | 2014-12-23 | 2015-03-25 | 长春建筑学院 | Method for treating lignite upgrading wastewater by anaerobic co-metabolism |
| CN104496122A (en) * | 2014-12-23 | 2015-04-08 | 吉林建筑大学 | Method for treating lignite upgrading wastewater by virtue of micro-aerobic co-metabolism |
| CN105274178A (en) * | 2014-07-10 | 2016-01-27 | 江苏加德绿色能源有限公司 | Method for ex-situ preparation of methane and combined production of humic acid, and composite microbial agent used in same |
-
2017
- 2017-04-28 CN CN201710293650.3A patent/CN107058451B/en not_active Expired - Fee Related
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580851A (en) * | 2009-06-19 | 2009-11-18 | 西安科技大学 | Method for directionally transforming low-deterioration coal through photo-bio coupling |
| CN102822346A (en) * | 2009-12-18 | 2012-12-12 | 西里斯能源公司 | Biogasification of coal to methane and other useful products |
| US9102953B2 (en) * | 2009-12-18 | 2015-08-11 | Ciris Energy, Inc. | Biogasification of coal to methane and other useful products |
| CN102645396A (en) * | 2012-05-11 | 2012-08-22 | 太原理工大学 | Test method for improving coal rock permeability and device thereof |
| CN102900411A (en) * | 2012-10-29 | 2013-01-30 | 河南理工大学 | Biological permeability-increasing method for coal reservoir |
| CN103045652A (en) * | 2012-11-14 | 2013-04-17 | 山西晋城无烟煤矿业集团有限责任公司 | Method for converting brown coal into methane by utilizing microorganism |
| CN105274178A (en) * | 2014-07-10 | 2016-01-27 | 江苏加德绿色能源有限公司 | Method for ex-situ preparation of methane and combined production of humic acid, and composite microbial agent used in same |
| CN104445592A (en) * | 2014-12-23 | 2015-03-25 | 吉林建筑大学 | Method for treating lignite quality-improving waste water through aerobic cometabolism |
| CN104445610A (en) * | 2014-12-23 | 2015-03-25 | 长春建筑学院 | Method for treating lignite upgrading wastewater by anaerobic co-metabolism |
| CN104496122A (en) * | 2014-12-23 | 2015-04-08 | 吉林建筑大学 | Method for treating lignite upgrading wastewater by virtue of micro-aerobic co-metabolism |
Non-Patent Citations (4)
| Title |
|---|
| 厌氧微生物降解原煤体吸附甲烷试验研究;张瑞林 等;《煤炭科学技术》;20160531;第44卷(第5期);第155-159页 * |
| 微生物增产煤层气菌种的驯化;林海 等;《煤炭学报》;20120831;第37卷(第8期);第1359-1363页 * |
| 煤炭生物降解的最新研究进展;邵雪嫚 等;《中州煤炭》;20161231(第3期);第123-125页 * |
| 黄孢原毛平革菌在煤体中代谢_传输的实验研究;牛煜;《中国博士学位论文全文数据库 工程科技I辑》;20190815(第8期);第B021-13页 * |
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