CN107058451A - Using complex microbial inoculum Degradation and Transformation low-order coal to increase production the method for coal bed gas - Google Patents
Using complex microbial inoculum Degradation and Transformation low-order coal to increase production the method for coal bed gas Download PDFInfo
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Abstract
The invention discloses a kind of utilization complex microbial inoculum Degradation and Transformation low-order coal to increase production the method for coal bed gas, comprise the following steps：Enrichment and domestication, the preparation of aerobic composite bacteria agent, the preparation of anaerobism composite bacteria agent, the preparation of nutrient solution, the collection of coal bed gas, the coal bed gas collection of strain finish the processing in rear gas production coal seam.The present invention gives a kind of method that aerobic composite bacteria agent and anaerobism composite bacteria agent are injected to low order coal seam stage by stage, its object is to alternately realize aerobic and anaerobic environment, to accelerate molten coal and methane phase, so as to realize that the high-efficiency cleaning of low-order coal is utilized.In addition, the present invention gives the acclimation method, culture medium prescription and nutrient solution prescription of the corresponding composite bacteria agent of different phase, the extraction rate of biological coal bed gas is improved.
Description
Technical field
Biological coal bed gas technical field is produced the invention belongs to microorganism, and in particular to one kind utilizes complex microbial inoculum
Degradation and Transformation low-order coal is to increase production the method for coal bed gas.
Background technology
Low-order coal refers to the relatively low coal of degree of coalification, particularly gentle including lignite, jet coal, dross coal, weakly caking coal
Coal.China's low-order coal rich reserves, account for the 46% of national coal reserves, wherein explored lignite reserves has reached
101300000000 tons, but for a long time, low-order coal is because its calorific value is low, water content is high, oxygen content is high, easy weathering or spontaneous combustion, stable
Property it is poor, be not suitable for using as power fuel, not only fringe cost is high and easily causes environmental pollution for exploitation, do not adopt and cause energy
The waste in source, the exploitation for low-order coal faces a difficult selection condition, therefore how to be efficiently that the new of using energy source is asked using low-order coal
Topic.
In recent decades, with the theoretical confirmation of secondary biogas, coal seam Central Plains originally there is microbiologic population, can be by
Coal is converted into methane step by step from macromolecular to small molecule, and simply microorganism is by prime stratum ambient influnence, and gas production is low.Especially
Since scott proposes that secondary biogas is theoretical, the discovery of a large amount of efficient aerogenesis engineering bacterias, microorganism volume increase coal bed gas is used as one kind
New coal-bed-gas production-increase mode enters the visual field of people.Coal is, by Plant Evolution, particularly low-order coal, to usually contain big
The polycyclic fragrant organic matter of lignin structure is measured, existing research has confirmed that low-order coal is more easy to be degraded by microorganisms, and passed through
The 94%- of coal after microbiological treatment, the caloric value of coal-produced enterprise converted product and the caloric value of raw coal substantially raw coal
97%, and humic acid therein can increase in various degree, such as lignite after microbiological treatment in humic acid from raw coal
13.6% brings up to 25%~26%.As can be seen here, microbiological treatment low-order coal can not only produce the clean gas energy, and place
Coal sample after reason can further turn into resource of humic acid again.Therefore, to be not only low-order coal efficiently sharp for microorganism volume increase coal bed gas
The only choosing of new method, even more Coal Clean technology, Green Development.
Although prior art opens the research and spy that molten coal microorganism in situ degraded low-order coal produces biological methane gas
Rope, but there is not methane phase or the low phenomenon of methane phase efficiency in these inventions, analyze its reason after natural conditions injection：1. coal
Layer environment is big with laboratory condition difference, and the external source bacterium of injection is difficult in adapt under actual coal seam environment.Coal seam is subterranean coal
Preservation is deep mixed, and oxygen content, temperature, pH value, pressure are different；2. functional flora is incomplete during methane phase, or each stage
Microflora proportioning imbalance, plays flora synergy poor, it is impossible to continue methane phase；3. the flora of injection exists to the demand of oxygen
Difference, what is had is aerobic, some anaerobism, has plenty of amphimicrobe, and single step injection external source flora is not easy to the control of oxygen；4. produce first
Alkane bacterium is the final step of methane phase, and its quality and quantity is the key of methane phase, and coal seam origin methanogen is in primary ring
Obligate strictly anaerobic, slow-growing in border, and the few activity of quantity is low, even if substrate needed for having supplied methanogen by external source bacterium,
Limited by its quantity and activity, gas production and aerogenesis speed are still slow.Therefore, solve the above problems be can efficient degradation it is low
Rank coal and the key issue for being translated into methane.
The content of the invention
It is existing the invention provides a kind of utilization complex microbial inoculum Degradation and Transformation low-order coal to increase production the method for coal bed gas
There is technology although to open research and exploration that molten coal microorganism in situ degraded low-order coal produces biological methane gas, but micro- life
The problem of there is molten coal speed limit, not methane phase or low methane phase efficiency after injecting under field conditions (factors) in thing.
The invention provides a kind of utilization complex microbial inoculum Degradation and Transformation low-order coal to increase production the method for coal bed gas, its
It is characterised by, comprises the following steps：
Step 1, the preparation of microbial inoculum
Step 1.1, the enrichment and domestication of strain
Be inoculated in after Phanerochaete chrysosporium is activated in Phanerochaete chrysosporium culture medium carry out enrichment expand culture with
And domestication, after domestication 7-8 generations, obtain Phanerochaete chrysosporium zymotic fluid；
It is inoculated in after rhodopseudomonas spheroid is activated in rhodopseudomonas spheroid culture medium and carries out enrichment expansion culture and tame and docile
Change, after domestication 7-8 generations, obtain rhodopseudomonas spheroid zymotic fluid；
It is inoculated in after Pseudomonas cepacia is activated in Pseudomonas cepacia culture medium and carries out enrichment expansion culture and tame and docile
Change, after domestication 7-8 generations, obtain Pseudomonas cepacia zymotic fluid；
Step 1.2, the preparation of aerobic composite bacteria agent
Each component is weighed according to following parts by weight：6-11 parts of the Phanerochaete chrysosporium zymotic fluid, the ball are red false single
4-6 parts of born of the same parents' fermented liquid, 8-12 parts of the Pseudomonas cepacia zymotic fluid；Then each component is well mixed, that is, obtains described
Total plate count is 1.0 × 10 in aerobic composite bacteria agent, the aerobic composite bacteria agent6-1.0×1011cfu/mL；
Step 1.3, the preparation of anaerobism composite bacteria agent
Marsh gas fermentation pool sampling is inoculated in methanogen culture medium and carries out enrichment expansion culture, expands culture 7-8 generations
Afterwards, anaerobism composite bacteria agent is obtained；
Total plate count is 1.0 × 10 in the anaerobism composite bacteria agent6-1.0×1011cfu/mL；
Step 2, the preparation of nutrient solution
40g blackstrap, 8.5g potassium dihydrogen phosphates, 22g dipotassium hydrogen phosphates, 33g phosphoric acid hydrogen two are separately added into 1000g water
Sodium, 5g ammonium chlorides, stirring make each component obtain nutrient solution after dissolving；
Step 3, the collection of coal bed gas
The gas generation process that microorganism injects low-order coal is simulated with three axle seepage flow experiment systems, takes certified reference coal to be placed in reactor
In, setting shaft pressure is 3-6MPa, and confined pressure is 2-5MPa, aerobic compound toward injecting in reactor according to 3-6MPa osmotic pressure level pressure
Microbial inoculum, injection records injection rate after finishing, hereafter 3 days daily toward injection air 8-12min in reactor, when observing exit
Gas collection and liquid collecting are distinguished after gas-liquid separation, and determines gas production and gas componant, is less than 1% when detecting oxygen content in gas
When, anaerobism composite bacteria agent is injected into reactor with 3-6MPa osmotic pressure, and injection rate is recorded, reached when detecting methane content
When 30%, start to collect biological coal bed gas, when detecting methane content less than 5%, stop collecting biological coal bed gas, and inject
The injection rate of nutrient solution, wherein nutrient solution is aerobic composite bacteria agent and the 40-60% of anaerobism composite bacteria agent total injection rate, works as detection
When reaching 30% to methane content, then restart to collect biological coal bed gas；
When injecting nutrient solution and can not make that methane content reaches 5% in injection well, circulation injection is aerobic in the order described above
Composite bacteria agent, anaerobism composite bacteria agent and nutrient solution；
Step 4, coal bed gas collection finishes the processing in rear gas production coal seam
Certified reference coal after being finished through step 3 aerogenesis is recycled.
It is preferred that, include following components in every liter of Phanerochaete chrysosporium limit nitrogen culture medium：3.0g coal dusts, 2.0g pastes
Essence, 1.0g ammonium tartrates, 1.5mg vitamin B1s, 0.5g Tween 80s, 1.5g phenmethylols, 3.0g potassium dihydrogen phosphates, surplus is sterile
Water, pH is 5-6.
It is preferred that, following components are included in every liter of rhodopseudomonas spheroid culture medium：3.0g coal dusts, 1.0g yeast extracts,
1.0g ammonium chlorides, 0.2g dipotassium hydrogen phosphates, 0.3g tetrahydrate manganese chlorides, 2.0mg cupric sulfate pentahydrates, 0.5g sodium chloride, 1.0g carbonic acid
Hydrogen sodium, 2.0g sodium acetates, 3.0mg white vitriols, surplus is sterilized water.
It is preferred that, following components are included in every liter of Pseudomonas cepacia culture medium：3.0g coal dusts, 6.0g glucose,
2.0g beef extracts, 3.0g peptones, 5.0g sodium glutamates, 1.0g potassium dihydrogen phosphates, 0.2g magnesium sulfate, 0.1g potassium chloride, surplus
For sterilized water.
It is preferred that, particle diameter≤0.2mm of the coal dust.
It is preferred that, following components are included in every liter of methanogen culture medium：0.4g dipotassium hydrogen phosphates, 2.0g chlorinations
Magnesium, 0.4g potassium dihydrogen phosphates, 1.0g yeast immersion liquid, 1.0g ammonium chlorides, 2.0g sodium acetates, 0.2g potassium chloride, 2.0g sodium chloride,
10mL trace element solutions, surplus is sterilized water.
It is preferred that, to being finished through step 3 aerogenesis after certified reference coal carry out recycling specifically include：
The ature of coal of certified reference coal after to being finished through step 3 aerogenesis is analyzed, and is dropped if analyzing ash content in certified reference coal
As little as less than 10%, then show the coal seam containing the certified reference coal after bacterium solution in detaching coal seam, low culm in can exploiting；If
Humic acid composition increase in certified reference coal, then toward the diluted hydrochloric acid dissolution certified reference coal that injected slurry volume concentration in reactor is 10%,
And detach after the certified reference coal liquid of dissolving, humic acid purification is used.
Compared with prior art, the beneficial effects of the present invention are：
1) cooperative effect is presented in the complex micro organism fungicide that the present invention is provided, and can accelerate the enrichment of methanogen, shortens
The time of anaerobic gas generation, the especially low-order coal to cellulose, humus and all kinds of carbon containing macromoleculars in low-order coal coal seam have
Very strong degraded gas deliverability；In addition, complex micro organism fungicide method for implanting is simple and easy to apply in the present invention, it is low, green with putting into
The advantages of colour circle is protected, cleaned, and long action time, can effectively extend the gas well life-span.
2) in the present invention complex micro organism fungicide also to have effects that molten coal, molten coal aerogenesis can be effectively improved after terminating more
The hole quantity and the connectedness of hole of hole medium-coal so that hole, crack increase, have more superior compared with water filling, heat injection or pressure break
Antireflective effect.And this method also adds methane content in coal seam from source, the exploitation of low-order coal high-efficiency cleaning is not only
Good method, also increases production the good method of coal bed gas, can be generalized to for this in discarded coal bed gas gas well, goaf or mine tailing
Go.
Brief description of the drawings
Fig. 1 is composite bacteria agent preparation flow figure of the invention；
Fig. 2 is stage implementation result figure of the invention.
Embodiment
In order that those skilled in the art more fully understand that technical scheme can be practiced, with reference to specific
The invention will be further described for embodiment and accompanying drawing, but illustrated embodiment is not as a limitation of the invention.
Phanerochaete chrysosporium, rhodopseudomonas spheroid, Pseudomonas cepacia used are bought in Chinese agriculture in the present invention
Microbiological Culture Collection administrative center, experimental method described in following each embodiments is conventional method unless otherwise specified.
Embodiment 1
A kind of utilization complex microbial inoculum Degradation and Transformation low-order coal is comprised the following steps with increasing production the method for coal bed gas：
Step 1.1, the enrichment and domestication of strain
Be inoculated in after Phanerochaete chrysosporium is activated in Phanerochaete chrysosporium culture medium carry out enrichment expand culture with
And domestication, after 8 generations of domestication, obtain Phanerochaete chrysosporium zymotic fluid；
During Phanerochaete chrysosporium domestication culture, gradient sets domestication condition of culture, gradually steps up bacterial strain to hypoxemia, height
Pressure, the tolerance level of low temperature, specific domestication gradient condition, which is set, is shown in Table 1：
The Phanerochaete chrysosporium of table 1 tames gradient condition
It is inoculated in after rhodopseudomonas spheroid is activated in rhodopseudomonas spheroid culture medium and carries out enrichment expansion culture and domestication 8
Dai Hou, obtains rhodopseudomonas spheroid zymotic fluid；
It is inoculated in after Pseudomonas cepacia is activated in Pseudomonas cepacia culture medium and carries out enrichment expansion culture and tame and docile
Change, after 8 generations of domestication, obtain Pseudomonas cepacia zymotic fluid；
Wherein, when rhodopseudomonas spheroid, Pseudomonas cepacia domestication culture, gradient sets domestication condition of culture, gradually carries
High bacterial strain to hypoxemia, high pressure, low temperature tolerance level, specific domestication gradient condition and Phanerochaete chrysosporium domestication culture gradient
Condition is identical；
Step 1.2, the preparation of aerobic composite bacteria agent
Each component is weighed according to following parts by weight：8 parts of the Phanerochaete chrysosporium zymotic fluid, the rhodopseudomonas spheroid
5 parts of zymotic fluid, 10 parts of the Pseudomonas cepacia zymotic fluid；Then each component is mixed, stirred, that is, obtain described aerobic
Total plate count is 1.0 × 10 in composite bacteria agent, aerobic composite bacteria agent10cfu/mL；
Step 1.3, the preparation of anaerobism composite bacteria agent
Marsh gas fermentation pool sampling is inoculated in methanogen culture medium and carries out enrichment expansion culture, is expanded after 8 generations of culture,
Obtain anaerobism composite bacteria agent；
Total plate count is 1.0 × 10 in anaerobism composite bacteria agent10cfu/mL；
Step 2, the preparation of nutrient solution
40g blackstrap, 8.5g potassium dihydrogen phosphates, 22g dipotassium hydrogen phosphates, 33g phosphoric acid hydrogen two are separately added into 1000g water
Sodium, 5g ammonium chlorides, stirring make each component obtain nutrient solution after dissolving；
Step 3, the collection of coal bed gas
With three axle seepage flow experiment systems simulation microorganism injection lignite gas generation process, certified reference coal size is Φ 50mm х
100mm, quality is 251.2g, and axle pressure and confined pressure are respectively set to 5MPa and 4MPa, according to the past reaction of 4MPa osmotic pressure level pressure
Aerobic composite bacteria agent is injected in kettle, injection length is 5h, and injection rate is 420g, 10min of air is injected daily within hereafter 3 days,
Injection valve is closed in injection after finishing, gas collection and liquid collecting are distinguished after exit gas-liquid separation is observed, determines gas production gentle
Body composition, when detecting in aerogenesis oxygen content and being less than 1%, anaerobism composite bacteria agent is injected with 4MPa osmotic pressure, injection
Measure as 380g, injection valve is closed after injection, methane content in detection aerogenesis daily in continuous 20 days, when detecting methane content
When reaching 30%, start to collect biological coal bed gas, detect methane content in aerogenesis during collection daily, contain when detecting methane
When amount is less than 5%, stop collecting biological coal bed gas, and inject nutrient solution, the wherein injection rate of nutrient solution is aerobic composite bacteria agent
With the 60% of anaerobism composite bacteria agent total injection rate, when detecting methane content and reaching 30%, then restart to collect biological coal
Layer gas；
When injecting nutrient solution and can not make that methane content reaches 5% in injection well, circulation injection is aerobic in the order described above
Composite bacteria agent, anaerobism composite bacteria agent and nutrient solution；
Step 4, coal bed gas collection finishes the processing in rear gas production coal seam
The ature of coal of certified reference coal is analyzed after to being finished through step 3 aerogenesis, is analyzed content of ashes in certified reference coal and is
8.3%, then show that the coal seam containing the certified reference coal, can be with working seam after bacterium solution in detaching coal seam；If in certified reference coal
The increase of humic acid composition, then be 10% diluted hydrochloric acid dissolution certified reference coal, and detach the mark of dissolving toward implantation concentration in reactor
After quasi- coal sample liquid, humic acid purification is used.
Embodiment 2
Step 1, the preparation of microbial inoculum
Step 1.1, the enrichment and domestication of strain
Be inoculated in after Phanerochaete chrysosporium is activated in Phanerochaete chrysosporium culture medium carry out enrichment expand culture with
And domestication, after 7 generations of domestication, obtain Phanerochaete chrysosporium zymotic fluid；
During Phanerochaete chrysosporium domestication culture, gradient sets domestication condition of culture, gradually steps up bacterial strain to hypoxemia, height
Pressure, the tolerance level of low temperature, specific domestication gradient condition, which is set, is shown in Table 2：
The Phanerochaete chrysosporium of table 2 tames gradient condition
It is inoculated in after rhodopseudomonas spheroid is activated in rhodopseudomonas spheroid culture medium and carries out enrichment expansion culture and tame and docile
Change, after 7 generations of domestication, obtain rhodopseudomonas spheroid zymotic fluid；
It is inoculated in after Pseudomonas cepacia is activated in Pseudomonas cepacia culture medium and carries out enrichment expansion culture and tame and docile
Change, after 7 generations of domestication, obtain Pseudomonas cepacia zymotic fluid；
Wherein, when rhodopseudomonas spheroid, Pseudomonas cepacia domestication culture, gradient sets domestication condition of culture, gradually carries
High bacterial strain to hypoxemia, high pressure, low temperature tolerance level, specific domestication gradient condition and Phanerochaete chrysosporium domestication culture gradient
Condition is identical；
Step 1.2, the preparation of aerobic composite bacteria agent
Each component is weighed according to following parts by weight：11 parts of the Phanerochaete chrysosporium zymotic fluid, the red false unit cell of the ball
4 parts of fermented liquid, 8 parts of the Pseudomonas cepacia zymotic fluid；Then each component is well mixed, that is, obtains described aerobic compound
Total plate count is 1.0 × 10 in microbial inoculum, the aerobic composite bacteria agent11cfu/mL；
Step 1.3, the preparation of anaerobism composite bacteria agent
Marsh gas fermentation pool sampling is inoculated in methanogen culture medium and carries out enrichment expansion culture, is expanded after 7 generations of culture,
Obtain anaerobism composite bacteria agent；
Total plate count is 1.0 × 10 in the anaerobism composite bacteria agent11cfu/mL；
Step 2, the preparation of nutrient solution
40g blackstrap, 8.5g potassium dihydrogen phosphates, 22g dipotassium hydrogen phosphates, 33g phosphoric acid hydrogen two are separately added into 1000g water
Sodium, 5g ammonium chlorides, stirring make each component obtain nutrient solution after dissolving；
Step 3, the collection of coal bed gas
The gas generation process that microorganism injects lignite is simulated with three axle seepage flow experiment systems, it is Φ 50mm to take certified reference coal size
х 100mm, quality is 239.3g, and axle pressure and confined pressure are respectively set to 6MPa and 5MPa, according to 3MPa osmotic pressure level pressure toward instead
Answer and aerobic composite bacteria agent is injected in kettle, injection length is 6h, injection rate is 380g, air 12min, note are injected within hereafter 3 days daily
Enter to close injection valve after finishing, gas collection and liquid collecting are distinguished after exit gas-liquid separation is observed, determine gas production and gas
Composition, when detecting in gas oxygen content and being less than 1%, anaerobism composite bacteria agent is injected with 3MPa osmotic pressure, injection rate
For 360g, injection valve is closed after injection, methane content in aerogenesis is detected in continuous 20 days daily, is reached when detecting methane content
During to 30%, start to collect biological coal bed gas, methane content in detection aerogenesis daily during collection, when detecting methane content
During less than 5%, stop collecting biological coal bed gas, and inject nutrient solution, wherein the injection rate of nutrient solution be aerobic composite bacteria agent with
The 40% of anaerobism composite bacteria agent total injection rate, when detecting methane content and reaching 30%, then restarts to collect biological coal seam
Gas；
When injecting nutrient solution and can not make that methane content reaches 5% in injection well, circulation injection is aerobic in the order described above
Composite bacteria agent, anaerobism composite bacteria agent and nutrient solution；
Step 4, coal bed gas collection finishes the processing in rear gas production coal seam
The ature of coal of certified reference coal is analyzed after to being finished through step 3 aerogenesis, is analyzed content of ashes in certified reference coal and is
7.8%, then show the coal seam containing the certified reference coal after bacterium solution in detaching coal seam, low middle culm can be exploited；If standard coal
Humic acid composition increase in sample, then be 10% diluted hydrochloric acid dissolution certified reference coal, and detach dissolving toward implantation concentration in reactor
Certified reference coal liquid after, by humic acid purification be used.
It should be noted that including following components in every liter of Phanerochaete chrysosporium limit nitrogen culture medium：3.0g coal dusts, 2.0g
Dextrin, 1.0g ammonium tartrates, 1.5mg vitamin B1s, 0.5g Tween 80s, 1.5g phenmethylols, 3.0g potassium dihydrogen phosphates, surplus is nothing
Bacterium water, pH is 5-6；
Following components are included in every liter of rhodopseudomonas spheroid culture medium：3.0g coal dusts, 1.0g yeast extracts, 1.0g ammonium chlorides,
0.2g dipotassium hydrogen phosphates, 0.3g tetrahydrate manganese chlorides, 2.0mg cupric sulfate pentahydrates, 0.5g sodium chloride, 1.0g sodium acid carbonates, 2.0g vinegar
Sour sodium, 3.0mg white vitriols, surplus is sterilized water；
Following components are included in every liter of Pseudomonas cepacia culture medium：3.0g coal dusts, 6.0g glucose, 2.0g beef extracts,
3.0g peptones, 5.0g sodium glutamates, 1.0g potassium dihydrogen phosphates, 0.2g magnesium sulfate, 0.1g potassium chloride, surplus is sterilized water；
Particle diameter≤0.2mm of coal dust；
Following components are included in every liter of methanogen culture medium：0.4g dipotassium hydrogen phosphates, 2.0g magnesium chlorides, 0.4g di(2-ethylhexyl)phosphates
Hydrogen potassium, 1.0g yeast immersion liquid, 1.0g ammonium chlorides, 2.0g sodium acetates, 0.2g potassium chloride, 2.0g sodium chloride, 10mL trace elements
Solution, surplus is sterilized water.
The method of embodiment 1 and embodiment 2 utilizes complex microbial inoculum Degradation and Transformation low-order coal, greatly improves
Methane output.In order to illustrate the effect of embodiment 1 and embodiment 2, with experiment condition of the same race but aerobic micro- life is not injected into
Thing composite bacteria agent, is directly injected into the lignite of anaerobism microbial inoculum after domestication as reference, when the methane of three kinds of method for implanting of contrast is produced
Between, gas production rate and total gas production, concrete outcome is shown in Table 3：
The aerogenesis parameter of the complex microbial inoculum Degradation and Transformation low-order coal of table 3
| Sample | The initial aerogenesis time (d) | Gas production rate (mL/D.kg) | Gas production (L) |
| Embodiment 1 | 5 | 103.2 | 2.59 |
| Embodiment 2 | 5 | 80.7 | 1.93 |
| Control sample | 9 | 20.6 | 0.5 |
It is not added with giving birth in a subtle way from table 3 it can be seen that the gas production rate and gas production of embodiment 1 and embodiment 2 are significantly larger than
The gas production rate and gas production of the low-order coal of thing composite bacteria agent, therefore, the present invention are low using complex microbial inoculum Degradation and Transformation
Rank coal has obvious advantage to increase production the method for coal bed gas, can promote the use of.
Embodiment 1 and embodiment 2 inject the aerobic composite bacteria agent tamed in the first stage, are produced using aerobic composite bacteria agent
Raw anaerobism living environment and solvent increase the porosity and permeability in coal seam, so that the product after solvent can be subsequently implanted into
Anaerobe further convert；Then second stage injection anaerobism composite bacteria agent, the composite bacteria agent is assigned according to actual formation
The condition of depositing is tamed, and major function is efficient methane phase, under anaerobic turns the carbon dioxide and hydrogen of first stage
Turn to methane；Phase III injects nutrient solution, keeps microbial activity and maintains bacterium solution pH value.These three stages act synergistically,
Accelerate the enrichment of methanogen, shorten the time of anaerobic gas generation, especially to cellulose in low-order coal coal seam, humus and
The low-order coal of all kinds of carbon containing macromoleculars has very strong degraded gas deliverability.Therefore, the present invention is added in coal seam from source
Methane content, is not only the good method of low-order coal high-efficiency cleaning exploitation, also increases production the good method of coal bed gas, is that this can be with
It is generalized in discarded coal bed gas gas well, goaf or mine tailing.
When being related to number range in claims of the present invention, it is thus understood that two end points and two of each number range
Any one numerical value can select between individual end points, because the step method of use is identical with embodiment 1-2, in order to prevent going to live in the household of one's in-laws on getting married
State, description of the invention preferred embodiment, but those skilled in the art once know basic creative concept, then
Other change and modification can be made to these embodiments.So, appended claims are intended to be construed to include preferred embodiment
And fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention
God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies
Within be also intended to comprising these change and modification including.
Claims (7)
1. a kind of utilization complex microbial inoculum Degradation and Transformation low-order coal is to increase production the method for coal bed gas, it is characterised in that including
Following steps：
Step 1, the preparation of microbial inoculum
Step 1.1, the enrichment and domestication of strain
Phanerochaete chrysosporium training is inoculated in after Phanerochaete chrysosporium (Phanerochaete chrysosporium) is activated
Support and enrichment expansion culture is carried out in base and is tamed, after domestication 7-8 generations, obtain Phanerochaete chrysosporium zymotic fluid；
Rhodopseudomonas spheroid culture medium is inoculated in after rhodopseudomonas spheroid (Rhodopseudomonas spheroides) is activated
It is middle to carry out enrichment expansion culture and tame, after domestication 7-8 generations, obtain rhodopseudomonas spheroid zymotic fluid；
It is inoculated in after Pseudomonas cepacia (Pseudomonascepacia) is activated in Pseudomonas cepacia culture medium and carries out richness
Collection expands culture and tamed, and after domestication 7-8 generations, obtains Pseudomonas cepacia zymotic fluid；
Step 1.2, the preparation of aerobic composite bacteria agent
Each component is weighed according to following parts by weight：6-11 parts of the Phanerochaete chrysosporium zymotic fluid, the rhodopseudomonas spheroid
4-6 parts of zymotic fluid, 8-12 parts of the Pseudomonas cepacia zymotic fluid；Then each component is well mixed, that is, obtains described aerobic
Total plate count is 1.0 × 10 in composite bacteria agent, the aerobic composite bacteria agent6-1.0×1011cfu/mL；
Step 1.3, the preparation of anaerobism composite bacteria agent
Marsh gas fermentation pool sampling is inoculated in methanogen culture medium and carries out enrichment expansion culture, expands after culture 7-8 generations, obtains
To anaerobism composite bacteria agent；
Total plate count is 1.0 × 10 in the anaerobism composite bacteria agent6-1.0×1011cfu/mL；
Step 2, the preparation of nutrient solution
Be separately added into 1000g water 40g blackstrap, 8.5g potassium dihydrogen phosphates, 22g dipotassium hydrogen phosphates, 33g disodium hydrogen phosphates,
5g ammonium chlorides, stirring makes each component obtain nutrient solution after dissolving；
Step 3, the collection of coal bed gas
The gas generation process that microorganism injects low-order coal is simulated with three axle seepage flow experiment systems, takes certified reference coal to be placed in reactor,
Setting shaft pressure is 3-6MPa, and confined pressure is 2-5MPa, and the osmotic pressure level pressure according to 3-6MPa is toward injecting aerobic compound bacteria in reactor
Agent, injection records injection rate after finishing, hereafter 3 days daily toward injection air 8-12min in reactor, when observing exit gas
Gas collection and liquid collecting respectively after liquid separation, and gas production and gas componant are determined, it is less than 1% when detecting oxygen content in gas
When, anaerobism composite bacteria agent is injected into reactor with 3-6MPa osmotic pressure, and injection rate is recorded, reached when detecting methane content
When 30%, start to collect biological coal bed gas, when detecting methane content less than 5%, stop collecting biological coal bed gas, and inject
The injection rate of nutrient solution, wherein nutrient solution is aerobic composite bacteria agent and the 40-60% of anaerobism composite bacteria agent total injection rate, works as detection
When reaching 30% to methane content, then restart to collect biological coal bed gas；
When injecting nutrient solution and can not make that methane content reaches 5% in injection well, circulation injection is aerobic compound in the order described above
Microbial inoculum, anaerobism composite bacteria agent and nutrient solution；
Step 4, coal bed gas collection finishes the processing in rear gas production coal seam
Certified reference coal after being finished through step 3 aerogenesis is recycled.
2. utilization complex microbial inoculum Degradation and Transformation low-order coal according to claim 1 is to increase production the method for coal bed gas,
Characterized in that, including following components in every liter of Phanerochaete chrysosporium limit nitrogen culture medium：3.0g coal dusts, 2.0g dextrin,
1.0g ammonium tartrates, 1.5mg vitamin B1s, 0.5g Tween 80s, 1.5g phenmethylols, 3.0g potassium dihydrogen phosphates, surplus is sterilized water,
PH is 5-6.
3. utilization complex microbial inoculum Degradation and Transformation low-order coal according to claim 1 is to increase production the method for coal bed gas,
Characterized in that, including following components in every liter of rhodopseudomonas spheroid culture medium：3.0g coal dusts, 1.0g yeast extracts, 1.0g
Ammonium chloride, 0.2g dipotassium hydrogen phosphates, 0.3g tetrahydrate manganese chlorides, 2.0mg cupric sulfate pentahydrates, 0.5g sodium chloride, 1.0g sodium acid carbonates,
2.0g sodium acetates, 3.0mg white vitriols, surplus is sterilized water.
4. utilization complex microbial inoculum Degradation and Transformation low-order coal according to claim 1 is to increase production the method for coal bed gas,
Characterized in that, including following components in every liter of Pseudomonas cepacia culture medium：3.0g coal dusts, 6.0g glucose, 2.0g
Beef extract, 3.0g peptones, 5.0g sodium glutamates, 1.0g potassium dihydrogen phosphates, 0.2g magnesium sulfate, 0.1g potassium chloride, surplus is nothing
Bacterium water.
5. according to any described utilization complex microbial inoculum Degradation and Transformation low-order coals of claim 2-4 to increase production coal bed gas
Method, it is characterised in that particle diameter≤0.2mm of the coal dust.
6. utilization complex microbial inoculum Degradation and Transformation low-order coal according to claim 1 is to increase production the method for coal bed gas,
Characterized in that, including following components in every liter of methanogen culture medium：0.4g dipotassium hydrogen phosphates, 2.0g magnesium chlorides,
0.4g potassium dihydrogen phosphates, 1.0g yeast immersion liquid, 1.0g ammonium chlorides, 2.0g sodium acetates, 0.2g potassium chloride, 2.0g sodium chloride,
10mL trace element solutions, surplus is sterilized water.
7. utilization complex microbial inoculum Degradation and Transformation low-order coal according to claim 1 is to increase production the method for coal bed gas,
Specifically included characterized in that, the certified reference coal after to being finished through step 3 aerogenesis carries out recycling：
The ature of coal of certified reference coal after to being finished through step 3 aerogenesis is analyzed, and is reduced to if analyzing ash content in certified reference coal
Less than 10%, then show the coal seam containing the certified reference coal after bacterium solution in detaching coal seam, low culm in can exploiting；If standard
Humic acid composition increase in coal sample, then be 10% diluted hydrochloric acid dissolution certified reference coal, and take out toward injected slurry volume concentration in reactor
After the certified reference coal liquid of exsolution solution, humic acid purification is used.
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