CN215768245U - Water body comprehensive toxicity detection equipment based on floating algae - Google Patents
Water body comprehensive toxicity detection equipment based on floating algae Download PDFInfo
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- CN215768245U CN215768245U CN202121594575.2U CN202121594575U CN215768245U CN 215768245 U CN215768245 U CN 215768245U CN 202121594575 U CN202121594575 U CN 202121594575U CN 215768245 U CN215768245 U CN 215768245U
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Abstract
The utility model relates to the technical field of comprehensive toxicity detection of water bodies, and discloses comprehensive toxicity detection equipment of a water body based on floating algae, which avoids system cross contamination by using a precise sequential injection means, improves the measurement precision, reduces the reagent loss of the equipment, and reduces the later maintenance frequency of the equipment; in addition, the utility model can increase the measurement contrast group before the water sample to be detected on the basis of contrast of the toxicity blank water sample, avoid the interference factor of floating algae contained in the water sample to be detected, improve the inherent defect of measuring the toxicity content by the floating algae and realize the accurate detection of the comprehensive toxicity of the water body.
Description
Technical Field
The utility model relates to the technical field of comprehensive toxicity detection of water bodies, in particular to a comprehensive toxicity detection device of a water body based on floating algae.
Background
In recent years, the water environment pollution condition is more complex, pollution accidents caused by heavy metal and organic toxicant frequently occur, and the high importance on the water quality ecological environment safety is attracted at home and abroad. The problem of water quality safety evaluation cannot be solved by physicochemical analysis means such as toxic pollutant determination, concentration analysis and the like. Due to the fact that pollutants contained in water are different, a single physical and chemical index only reflects the content of a few substances, and comprehensive evaluation on the pollution conditions of all the substances cannot be conducted.
The traditional water quality indexes such as COD, ammonia nitrogen, total phosphorus and the like are characterized by the content level of one or a certain substance in the water body, and the comprehensive index reflecting the toxicity of the water body is lacked. Even if the physicochemical indexes of the discharged sewage reach the standard, the discharged sewage still has the possibility of generating toxic action on aquatic microorganisms and even brings potential risks to an aquatic ecosystem of a receiving water body. The aquatic organism toxicity monitoring technology is the evaluation means which can meet the comprehensive influence of various pollutants on the water environment at present.
At present, the toxicity early warning market of water bodies commonly adopts a photobacterium toxicity analysis method and an alga toxicity analysis method, but the photobacterium mainly applied at present comprises bright photobacterium, vibrio fischeri and vibrio qinghaiensis. Wherein the Vibrio brightens and Vibrio fischeri are marine luminophores, and Vibrio qinghaiensis is freshwater luminophores. However, the comprehensive toxicity analysis of the luminous bacteria actually applied to the online automatic monitoring of surface water basically adopts the marine luminous bacteria Vibrio fischeri. The measurement systems (including salinity, buffer systems and reaction time) of different strains have differences, the toxicity sensitivity and the test principle of different toxic compounds have certain differences, and the differences have advantages and disadvantages, so that the comparison research on the luminescence performance test of different strains is lacked at present.
In addition, aiming at the instability of the luminescent bacteria and the advantages of the algae in evaluating the comprehensive quality of the water body, the later development of the product based on the algae toxicity monitoring is wider, and the product has persuasion and authenticity for evaluating the comprehensive ecological environment of the water body in the later period. Compared with the traditional living biological receptor, the luminescent bacteria are good monitoring media. However, bacteria are susceptible to physique, and the activity of the bacteria can be influenced by weak changes of water, so that false message false alarm conditions often occur in field application, and the early warning capability of the bacteria is seriously influenced.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problems, the utility model provides a water body comprehensive toxicity detection device based on floating algae.
In order to solve the technical problems, the utility model adopts the following technical scheme:
a water body comprehensive toxicity detection device based on floating algae comprises a system toxicity detection module and a system flow path module;
the system flow path module includes:
the optical detection darkroom is used for containing a blank water sample, a water sample to be detected or a water sample to be detected added with an algae reagent;
a flow path pipe;
the power pump is arranged on the flow pipeline and used for conveying the water sample to be detected into the optical detection darkroom or emptying the water sample to be detected in the optical detection darkroom,
the reagent assembly is internally stored with an algae reagent;
a cleaning component, wherein a blank water sample is stored in the cleaning component;
the input end of the multi-channel switching valve is respectively communicated with the reagent component, the cleaning component and the flow channel pipeline, and the output end of the multi-channel switching valve is communicated with the optical detection darkroom; the device is used for controlling the communication relationship among the reagent assembly, the cleaning assembly, the flow channel pipeline and the optical detection darkroom;
the system toxicity detection module comprises:
the system light source is used for emitting light and emitting the light into the optical detection darkroom through the optical conduction medium to form incident light;
the photoelectric detection assembly comprises a detection surface used for detecting a water sample to be detected in the optical detection darkroom, and the detection surface is orthogonal to the plane where the incident light is located.
Furthermore, the light emitted by the system light source consists of matrix multi-wavelength monochromatic light; the wavelength of the matrix multi-wavelength monochromatic light can be used for carrying out fluorescence excitation on the algae.
Further, the wavelength range of the matrix multi-wavelength monochromatic light is 300-900 nm.
Further, the system light source has a matrix excitation mode capable of emitting matrix excitation light and a single-point excitation mode capable of emitting single-point excitation light; the matrix type excitation light is used for detecting the concentration distribution of the algae community contained in the water sample to be detected, so that the interference of the algae activity of the water sample to be detected on toxicity detection is avoided; the single-point excitation light can excite the water sample to be detected with the added alga reagent by adopting a fast phase excitation mode and a relaxation excitation mode to obtain the alga activity parameters of the water sample to be detected with the added alga reagent.
Compared with the prior art, the utility model has the beneficial technical effects that:
(1) the detection equipment in the utility model is communicated with the flow channel pipeline, the reagent component and the cleaning component through the multi-channel switching valve, and the cross contamination of the system is avoided by using a precise sequential injection means, so that the measurement precision is improved, the reagent loss of the equipment is reduced, and the later maintenance frequency of the equipment is reduced.
(2) The method overcomes the defects of the existing luminous bacterium water environment detection, improves the original defect of analyzing the comprehensive toxicity of the water body by depending on floating algae, fundamentally eliminates the interference factors of the external environment and improves the product accuracy.
(3) Two ends of the flow pipeline are respectively communicated with a water sample to be detected and the multi-channel switching valve, and the water sample to be detected can enter the optical detection darkroom and be discharged out of the optical detection darkroom through the power pump; the multichannel switching valve controls the algae reagent or the blank water sample to enter the optical detection darkroom, and the blank water sample in the cleaning assembly can be used as cleaning water to clean the optical detection darkroom; the utility model has the functions of automatic sample introduction, automatic cleaning and automatic waste discharge, and can meet the application requirement of real-time online water environment monitoring; the method can detect the concentration distribution of the algal communities of the water sample to be detected on the basis of the comparison of the traditional toxicity blank sample, avoids the interference of floating algae contained in the water sample to be detected and realizes the accurate detection of the comprehensive toxicity of the water body.
Drawings
FIG. 1 is a schematic block diagram of the detection apparatus of the present invention.
Detailed Description
A preferred embodiment of the present invention will be described in detail below with reference to the accompanying drawings.
The detection device comprises a system toxicity detection module 1 and a system flow path module 2; the system toxicity detection module 1 comprises an upper computer 11, a control acquisition center 12, a driving circuit 13, a system light source 14, a photoelectric detection assembly 16 and a signal processor 15; the system flow path module 2 includes: a power pump 21, a flow channel pipeline 22, a reagent assembly 23, a cleaning assembly 24, an optical detection darkroom 25 and a multi-channel switching valve 26.
Based on the setting of the upper computer 11, the collection center 12 is controlled to control all components of the system flow path module 2 to act, the power pump 21 is combined with the flow path pipeline 22 to realize the extraction and the emptying of the water sample to be detected, and the multi-channel switching valve 26 is used for switching the channels of the blank water sample, the algae reagent and the water sample to be detected; the outlet of the flow path of the multi-channel switching valve 26 is connected with the optical detection darkroom 25, so that a blank water sample, a water sample to be detected and an algae reagent can enter the optical detection darkroom; wherein the blank water sample can be used as cleaning water.
The system toxicity detection module 1 has a function of detecting physiological states of floating algae in a water body, and the upper computer 11 is connected with the control acquisition center 12 through a protocol and can write parameters of the control acquisition center; the output end of the control acquisition center 12 is connected with the input end of the driving circuit 13; the output terminal of the drive circuit 13 is connected to the input terminal of the system light source 14, and the system light source can be turned on. The system light source 14 is coupled to the photodetection assembly 16 through an optically conductive medium 17. The detection surface of the photoelectric detection component 16 and the incident surface of the system light source are vertically distributed in a three-dimensional manner; the output end of the photoelectric detection assembly 16 is connected with the input end of the control acquisition center 12 through the output end of the signal processor 15; and the reading end of the upper computer 11 is interconnected with the transmission end of the control acquisition center 12 and is used for displaying the model and outputting the measurement result.
The light emitted by the system light source 14 is composed of matrix multi-wavelength monochromatic light, the wavelength range is 300nm to 900nm, and the requirement of common algal community fluorescence excitation can be met; the system light source has two working modes, namely a matrix type excitation mode and a single-point type excitation mode. The matrix type exciting light is used for detecting the concentration distribution of the algae community contained in the water sample to be detected, and the interference of the algae activity of the water sample to be detected on toxicity measurement is avoided. The single-point excitation light excites the water sample to be detected, in which the algae reagent is added, through two excitation modes of fast phase and relaxation, to obtain the algae activity parameters of the water sample to be detected.
The detection method comprises the following steps:
step A: fixedly mounting all components of the detection equipment, starting a man-machine interaction interface through an upper computer 11, and setting a control acquisition center 12 to control a system flow path module 1: the power pump 21 and the flow pipeline 22 are driven to finish the extraction of the water sample to be detected, and the extracted water sample to be detected flows into the optical detection darkroom 25 through the flow pipeline 22 and the multi-channel switching valve 26 to wait for detection;
and B: the upper computer 11 is communicated with the control acquisition center 12, and the driving circuit 13 is triggered to switch the system light source 14 to a matrix type excitation mode; matrix type exciting light emitted by a system light source is emitted into an optical detection darkroom 25 through an optical conduction medium 17, and the photoelectric detection assembly 16 detects and obtains the concentration distribution of the own algal community of the water sample to be detected;
and C: controlling the multi-channel switching valve 26 to communicate the input end of the reagent module 23 with the input end of the optical detection darkroom 25, and injecting the algae reagent into the water sample to be detected in the optical detection darkroom 25; the upper computer 11 is communicated with the control acquisition center 12, the trigger driving circuit 13 lights the system light source 14, the single-point excitation mode is switched, and the system light source is in two excitation modes of fast phase and relaxation through an internal excitation mechanism; similarly, single-point excitation light emitted by the system light source is incident to the optical detection darkroom 25 through the optical conduction medium 17, the photoelectric detection assembly 16 detects the change of fluorescence signals of the algae reagent in two excitation modes, and the fluorescence signals are further processed by the signal processor 15;
step D: detecting the concentration distribution of the algal communities of the blank water sample and the algal activity parameters after the addition of the algal reagent in the same manner as in the step B, C to obtain a fluorescence signal change model of the blank water sample;
step E: the concentration distribution of the own algal community of the water sample to be detected is used as the substrate value of the algal concentration of the water sample to be detected, the amount of the added algal reagent can be obtained in the step C, the actual algal content of the substrate removed in the water sample to be detected with the added algal reagent can be calculated, and the algal reagent activity parameter of the water sample to be detected is obtained; obtaining the activity parameters of the blank water sample algae reagent in the same way;
step F: comparing the activity parameters of the algae reagents in the water sample to be detected and the blank water sample, and further calculating by means of a pre-established fluorescence kinetic curve to obtain various photosynthesis parameters reflecting the biological physiological state; the photosynthesis parameters include photosynthesis photochemical quantum yield, photochemical efficiency, photosynthetic electron conduction rate, and the like.
The proposed fluorescence kinetic curve comprises the following steps:
step F1: preparing a series of blanks of the same volume of deionized water;
step F2: preparing an atrazine standard solution in a gradient range;
step F3: adding an equal amount of atrazine standard solution into a blank sample to form an experimental sample, and reserving a blank sample;
step F4: adding equal amount of culture algae reagent solution into each experimental sample and a blank sample to form a sample to be detected;
step F5: detecting an algae activity parameter for each sample to be tested by step A, B, C, D;
step F6: drawing respective fluorescence dynamics curves based on the algae activity parameters of the samples to be detected, and further obtaining photosynthesis parameters reflecting the physiological state of algae, such as fluorescence quantum yield and the like;
step F7: comparing the fluorescence dynamics curves of the samples to be detected and the blank samples to be detected in each experiment, and combining the change before and after the photosynthesis parameter, the photosynthesis parameter variation can replace each fluorescence dynamics curve, and further the strength relation of various water samples to be detected for inhibiting the photosynthesis activity of algae can be inverted.
Step F8: in order to further quantify the comprehensive toxicity, the toxic reagent, namely atrazine dosage can be preset before the experiment, the one-to-one corresponding relation between the change of the atrazine toxic dosage and the change of the relative photosynthesis parameters can be obtained, and the comprehensive water sample toxicity content relative to the atrazine dosage can be inverted.
The utility model avoids the cross contamination of the system by using a precise sequential injection means, improves the measurement precision, reduces the reagent loss of the equipment and reduces the later maintenance frequency of the equipment; in addition, on the basis of comparison of a toxicity blank water sample, a measurement comparison group before the water sample to be detected can be added, interference factors of floating algae contained in the water sample to be detected are avoided, the inherent defect of measuring the toxicity content by the floating algae is overcome, and accurate detection of comprehensive toxicity of the water body is realized.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (4)
1. A water body comprehensive toxicity detection device based on planktonic algae is characterized by comprising a system toxicity detection module and a system flow path module;
the system flow path module includes:
the optical detection darkroom is used for containing a blank water sample, a water sample to be detected or a water sample to be detected added with an algae reagent;
a flow path pipe;
the power pump is arranged on the flow pipeline and used for conveying the water sample to be detected into the optical detection darkroom or emptying the water sample to be detected in the optical detection darkroom,
the reagent assembly is internally stored with an algae reagent;
a cleaning component, wherein a blank water sample is stored in the cleaning component;
the input end of the multi-channel switching valve is respectively communicated with the reagent component, the cleaning component and the flow channel pipeline, and the output end of the multi-channel switching valve is communicated with the optical detection darkroom; the device is used for controlling the communication relationship among the reagent assembly, the cleaning assembly, the flow channel pipeline and the optical detection darkroom;
the system toxicity detection module comprises:
the system light source is used for emitting light and emitting the light into the optical detection darkroom through the optical conduction medium to form incident light;
the photoelectric detection assembly comprises a detection surface used for detecting a water sample to be detected in the optical detection darkroom, and the detection surface is orthogonal to the plane where the incident light is located.
2. The planktonic algae-based water body comprehensive toxicity detection apparatus according to claim 1, wherein: the light emitted by the system light source consists of matrix multi-wavelength monochromatic light; the wavelength of the matrix multi-wavelength monochromatic light can be used for carrying out fluorescence excitation on the algae.
3. The planktonic algae-based water body comprehensive toxicity detection apparatus according to claim 2, wherein: the wavelength range of the matrix type multi-wavelength monochromatic light is 300-900 nm.
4. The planktonic algae-based water body comprehensive toxicity detection apparatus according to claim 1, wherein: the system light source is provided with a matrix type excitation mode capable of emitting matrix type excitation light and a single-point type excitation mode capable of emitting single-point type excitation light; the matrix type excitation light is used for detecting the concentration distribution of the algae community contained in the water sample to be detected, so that the interference of the algae activity of the water sample to be detected on toxicity detection is avoided; the single-point excitation light can excite the water sample to be detected with the added alga reagent by adopting a fast phase excitation mode and a relaxation excitation mode to obtain the alga activity parameters of the water sample to be detected with the added alga reagent.
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| CN202121594575.2U CN215768245U (en) | 2021-07-12 | 2021-07-12 | Water body comprehensive toxicity detection equipment based on floating algae |
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| CN202121594575.2U CN215768245U (en) | 2021-07-12 | 2021-07-12 | Water body comprehensive toxicity detection equipment based on floating algae |
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