CN104374757A - Device for measuring delayed fluorescence decay of algae - Google Patents
Device for measuring delayed fluorescence decay of algae Download PDFInfo
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- CN104374757A CN104374757A CN201410618606.1A CN201410618606A CN104374757A CN 104374757 A CN104374757 A CN 104374757A CN 201410618606 A CN201410618606 A CN 201410618606A CN 104374757 A CN104374757 A CN 104374757A
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- algae
- quartz
- pond
- alga
- inhibiting agent
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Abstract
The invention discloses a device for measuring delayed fluorescence decay of algae. The device is structurally characterized in that a semi-flowing alga culture medium (1) is connected with a three-way valve (9) by virtue of a first micro peristaltic pump (2), the three-way valve (9) is communicated with a quartz excitation tank (5) on one hand and is connected to an alga inhibiting agent injector (3) on the other hand, the quartz excitation tank (5) is connected with an LED red light lamp (4) by virtue of an optical fiber and is connected with a quartz reaction tank (8) by virtue of a second micro peristaltic pump (6), and the quartz reaction tank (8) is connected with a fluorescence spectrum detector (10) by virtue of an optical fiber. The device disclosed by the invention can be used for continuously detecting the fluorescence decay of blank alga samples and alga samples with an alga inhibiting agent by virtue of the alga inhibiting agent injector, and can be used for characterizing the influence degree of the alga inhibiting agent on floating algae by virtue of integrals of attenuation of photons emitted within 90 seconds. According to the device disclosed by the invention, delayed fluorescence for characterizing alga growth inhibition can be rapidly measured, the precision and reproducibility of experiments can be improved, and the consumption of the alga inhibiting agent can be saved.
Description
Technical field
The present invention relates to a kind of device of substance-measuring, particularly relate to the determinator of planktonic algae in a kind of water, be specifically related to the device of a kind of Fast Measurement algae by algae-inhibiting agent influence degree.
Background technology
The excess emissions of the sewage that nitrogen and phosphorus content is higher, cause the aggravation of body eutrophication, the frequent outburst of wawter bloom algae becomes the focus of global concern, and the suppression of planktonic algae growth solves the key of water pollution problem, is also be correlated with the emphasis of scholar's research in the whole world.The birth of algae-inhibiting agent few in number makes the growth of algae obtain suppression to a certain degree.At present, by the detection of frustule quantity and chlorophyll a can be rough sign algae be subject to algae-inhibiting agent effect.But this testing process is complicated, speed is slow, reagent consumption amount is large.
Summary of the invention
Technical matters to be solved by this invention is just to provide a kind of device measuring the decay of algae delayed fluorescence, it can avoid decline frustule to cause impact when detecting, reach the degree characterizing algae-inhibiting agent exactly and algal grown is suppressed, overcome the problems such as testing process in prior art is complicated, speed is slow, algae-inhibiting agent consumption is large.
Technical matters to be solved by this invention is realized by such technical scheme, it includes half flowing algae media, algae-inhibiting agent syringe, LED red globe lamp, quartz excites pond, quartz reaction pond, fluoroscopic examination spectrometer sum-product intergrator, be placed in half flowing algae media of simple glass bottle by the first miniature peristaltic pump connecting tee valve, T-valve is connected quartz on the one hand and is excited pond, access algae-inhibiting agent syringe on the other hand, quartz excites pond by Fiber connection LED red globe lamp, quartz excites pond to connect quartz reaction pond by the second miniature peristaltic pump, quartz reaction pond is by Fiber connection fluorescence spectrum detector, fluorescence spectrum detector electrical connection integrator.
Principle of work of the present invention is: the algae liquid to be measured in half flowing algae media is evacuated to quartz by the first miniature peristaltic pump and excites pond, and quartz excites pond and LED red globe lamp by Fiber connection, and by the irradiation of LED red globe lamp, quartz excites the algae sample in pond to produce fluorescence; Excite the algae sample be stimulated in pond to be evacuated to quartz reaction pond in quartz by the second miniature peristaltic pump again, produce the quantity of photon after the fluorescence spectrum detector energy continuous detecting algae liquid be connected is stimulated, and record the change of photon numbers in 90s with quartz reaction pond;
Algae-inhibiting agent adds in the algae liquid to be measured of flowing by the unlatching of T-valve, quartz reaction pond is evacuated to after the algae liquid being added with algae-inhibiting agent excites by LED red globe lamp, after adding algae-inhibiting agent, the photolytic activity of planktonic algae reduces, the fluorescence produced that is stimulated can be decayed, by the attenuation change of photon in fluorescence spectrum detector record 90s, finally calculated the total amount difference DELTA DF of the delay decaying phosphor of suppressed planktonic algae and not suppressed planktonic algae by integrator.
Compared with prior art, detection precision of the present invention is high, and detection speed is fast, can realize full automation, and algae-inhibiting agent consumption is few, avoids and repeatedly adds algae-inhibiting agent, the trouble repeatedly measured, and makes the reproducible of experiment.Many measure can be realized by changing fluoroscopic examination spectrometer, algae-inhibiting agent etc.
Accompanying drawing explanation
Fig. 1 is functional structure chart of the present invention.
In figure: 1. half flowing algae media; 2. the first miniature peristaltic pump; 3. algae-inhibiting agent syringe; 4.LED red globe lamp; 5. quartz excites pond; 6. the second miniature peristaltic pump; 7. be with the micro constant-temperature case of thermostat; 8. quartz reaction pond; 9. three-way valve; 10. fluorescence spectrum detector; 11. integrators; 12. first waste liquid exhaust valves; 13. second waste liquid exhaust valves; 14. first waste collection bottles; 15. second waste collection bottles.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described:
As shown in Figure 1, the present invention includes half flowing algae media 1, algae-inhibiting agent syringe 3, LED red globe lamp 4, quartz excites pond 5, quartz reaction pond 8, fluoroscopic examination spectrometer 16 sum-product intergrator 17, be placed in half flowing algae media 1 of simple glass bottle by the first miniature peristaltic pump 2 connecting tee valve 9, T-valve 9 one aspect is connected quartz and is excited pond 5, access algae-inhibiting agent syringe 3 on the other hand, quartz excites pond 5 by Fiber connection LED red globe lamp 4, quartz excites pond 5 to connect quartz reaction pond 8 by the second miniature peristaltic pump 6, quartz reaction pond 8 is by Fiber connection fluorescence spectrum detector 10, fluorescence spectrum detector 10 is electrically connected integrator 11.
Described quartz reaction pond 8 is placed in the constant temperature oven 7 with thermostat, temperature with the constant temperature oven of thermostat is set to 25 DEG C ± 1, constant temperature oven with thermostat adopts light-proof material to make, both temperature of reaction was controlled, make again quartz reaction pond be in dark state, be convenient to measure algae under dark state and be stimulated and produce the change of photon numbers.
Quartz excites on pond 5 and first waste liquid exhaust valve 12 is housed, and quartz reaction pond 8 is equipped with the second waste liquid exhaust valve 13, terminates the rear or rear smooth delivery detection device of residual waste solution of instrument cleaning, make pick-up unit carry out many measure to realize experiment.
First miniature peristaltic pump 2 and the second miniature peristaltic pump 6 adopt acid-proof hose, avoid acid algae-inhibiting agent to the corrosion of connecting pipe, adopt adjustable speed, gageable miniature peristaltic pump, have pollution-free, precision is high, good airproof performance, be easy to the advantages such as maintenance; First miniature peristaltic pump 2 for aspirating algae liquid to be measured from half flowing algae media 1; The algae liquid be stimulated is evacuated to quartz reaction pond 8 for exciting pond 5 from quartz by the second miniature peristaltic pump 6.Described algae-inhibiting agent syringe 3 is converted by micro syringe, shading treatment is carried out to this syringe, reduce the decomposition of algae-inhibiting agent in course of reaction as far as possible, and quantize the consumption of algae-inhibiting agent by syringe, algae-inhibiting agent syringe 3 realizes the interpolation of algae-inhibiting agent by the keying of T-valve 9.The wavelength coverage of LED red globe lamp 4 is 640nm-770nm; Described integrator 11 can be connected with computer, realizes automatically calculating.
The present invention is rapidity and the stability of continuous detecting, the device of low consumption of a set of comprehensive utilization flow injection.The delay decaying phosphor (Δ DF) responded algae-inhibiting agent mensuration planktonic algae is divided into three steps:
The first step closes connected T-valve 9 valve of algae-inhibiting agent syringe 3, close quartzy the first waste liquid exhaust valve 12 excited on pond 5, open the second waste liquid exhaust valve 13 in quartz reaction pond 8, open LED red globe lamp 4, open the first miniature peristaltic pump 2 and the second miniature peristaltic pump 6, algae sample to be measured is allowed to excite pond 5 by the first miniature peristaltic pump 2 to quartz, the algae sample after exciting by LED red globe lamp 4 in pond 5 is excited to be evacuated to the quartz reaction pond 8 in the constant temperature oven 7 of thermostat by the second miniature peristaltic pump 6 at quartz, fluorescence spectrum detector 10 records this algae in moment and is stimulated photon amount produced, obtain initial value C
1.Open quartz and excite the first waste liquid exhaust valve 12 on pond 5, enter the first waste collection bottle 14 by surveying algae liquid, the second waste liquid exhaust valve 13 in quartz reaction pond 8 enters the second waste collection bottle 15 by surveying algae liquid.
Second step closes the first waste liquid exhaust valve 12 and the second waste liquid exhaust valve 13, device keeps above-mentioned state, when behind the quartz reaction pond 8 that algae liquid to be measured is evacuated in the constant temperature oven 7 being with thermostat by the second miniature peristaltic pump 6, close the first miniature peristaltic pump 2 and the second miniature peristaltic pump 6, recorded the change C of the photon amount of algae liquid in 90s quartz reaction pond 8 by fluorescence spectrum detector 10
i, opening the first waste liquid exhaust valve 12 and the second waste liquid exhaust valve 13 after 90s, entering the first waste collection bottle 14, second waste collection bottle 15 by surveying algae liquid.
3rd step closes the first waste liquid exhaust valve 12 and the second waste liquid exhaust valve 13, open algae-inhibiting agent syringe 3 connected T-valve 9 valve, open LED red globe lamp, open the first miniature peristaltic pump 2 and the second miniature peristaltic pump 6, make the flowing in the apparatus that the algae liquid to be measured in half flowing algae media 1 continues, by algae-inhibiting agent syringe 3, algae-inhibiting agent is injected the algae liquid of flowing simultaneously, biased sample excites exciting by LED red globe lamp 4 in pond 5 at quartz, after exciting the biased sample be stimulated in pond 5 to be evacuated to quartz reaction pond 8 in quartz by the second miniature peristaltic pump 6, close the first miniature peristaltic pump 2 and the second miniature peristaltic pump 6, the change A of the photon amount of algae liquid in quartz reaction pond 8 in 90s is recorded by fluorescence spectrum detector 10
i, opening the first waste liquid exhaust valve 12 and the second waste liquid exhaust valve 13 after 90s, entering the first waste collection bottle 14, second waste collection bottle 15 by surveying algae liquid.By the photon amount integration of integrator 11 couples of 90s.Finally draw the delay decaying phosphor value Δ DF that planktonic algae affects by algae-inhibiting agent.
The first waste liquid exhaust valve 12 and the second waste liquid exhaust valve 13 effluent discharge can be opened after experiment terminates or after cleaning device.
Above embodiment does not form the restriction to patent of the present invention, and all employings are equal to the technical scheme that replacement or the form of equivalent deformation obtain, within the protection domain all dropping on patent of the present invention.
Claims (4)
1. one kind measures the device of algae delayed fluorescence decay, it is characterized in that: include half flowing algae media (1), algae-inhibiting agent syringe (3), LED red globe lamp (4), quartz excites pond (5), quartz reaction pond (8), fluoroscopic examination spectrometer (16) sum-product intergrator (17), be placed in the algae media that partly flows (1) of simple glass bottle by the first miniature peristaltic pump (2) connecting tee valve (9), T-valve (9) is connected quartz on the one hand and is excited pond (5), access algae-inhibiting agent syringe (3) on the other hand, quartz excites pond (5) by Fiber connection LED red globe lamp (4), quartz excites pond (5) to connect quartz reaction pond (8) by the second miniature peristaltic pump (6), quartz reaction pond (8) is by Fiber connection fluorescence spectrum detector (10), fluorescence spectrum detector (10) electrical connection integrator (11).
2. a kind of device measuring the decay of algae delayed fluorescence according to claim 1, is characterized in that: described quartz reaction pond (8) is placed in the constant temperature oven (7) with thermostat.
3. a kind of device measuring the decay of algae delayed fluorescence according to claim 1, it is characterized in that: described quartz excites on pond (5) and the first waste liquid exhaust valve (12) is housed, and described quartz reaction pond (8) is equipped with the second waste liquid exhaust valve (13).
4. a kind of device measuring the decay of algae delayed fluorescence according to claim 1, is characterized in that: the first miniature peristaltic pump (2) and the second miniature peristaltic pump (6) adopt acid-proof hose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410618606.1A CN104374757B (en) | 2014-11-06 | 2014-11-06 | Device for measuring delayed fluorescence decay of algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410618606.1A CN104374757B (en) | 2014-11-06 | 2014-11-06 | Device for measuring delayed fluorescence decay of algae |
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| CN104374757A true CN104374757A (en) | 2015-02-25 |
| CN104374757B CN104374757B (en) | 2017-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410618606.1A Expired - Fee Related CN104374757B (en) | 2014-11-06 | 2014-11-06 | Device for measuring delayed fluorescence decay of algae |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3605598A1 (en) * | 1986-02-21 | 1987-08-27 | Degussa | Method and device for measuring the fluorescence decay time (afterglow time) of a fluorescing substance |
| US5757013A (en) * | 1995-12-06 | 1998-05-26 | American Research Corporation Of Virginia | Fluorescence decay measurement by calculation of inner product |
| CN1920535A (en) * | 2006-09-13 | 2007-02-28 | 哈尔滨工程大学 | Device for measuring fluorescence life time |
| CN103364378A (en) * | 2013-03-05 | 2013-10-23 | 江苏天瑞仪器股份有限公司 | Multifunctional joint sampling system for atomic fluorescence spectrometer |
-
2014
- 2014-11-06 CN CN201410618606.1A patent/CN104374757B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3605598A1 (en) * | 1986-02-21 | 1987-08-27 | Degussa | Method and device for measuring the fluorescence decay time (afterglow time) of a fluorescing substance |
| US5757013A (en) * | 1995-12-06 | 1998-05-26 | American Research Corporation Of Virginia | Fluorescence decay measurement by calculation of inner product |
| CN1920535A (en) * | 2006-09-13 | 2007-02-28 | 哈尔滨工程大学 | Device for measuring fluorescence life time |
| CN103364378A (en) * | 2013-03-05 | 2013-10-23 | 江苏天瑞仪器股份有限公司 | Multifunctional joint sampling system for atomic fluorescence spectrometer |
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| CN104374757B (en) | 2017-01-18 |
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