CN104374757B - Device for measuring delayed fluorescence decay of algae - Google Patents
Device for measuring delayed fluorescence decay of algae Download PDFInfo
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- CN104374757B CN104374757B CN201410618606.1A CN201410618606A CN104374757B CN 104374757 B CN104374757 B CN 104374757B CN 201410618606 A CN201410618606 A CN 201410618606A CN 104374757 B CN104374757 B CN 104374757B
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- algae
- quartz
- pond
- alga
- inhibiting agent
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Abstract
The invention discloses a device for measuring delayed fluorescence decay of algae. The device is structurally characterized in that a semi-flowing alga culture medium (1) is connected with a three-way valve (9) by virtue of a first micro peristaltic pump (2), the three-way valve (9) is communicated with a quartz excitation tank (5) on one hand and is connected to an alga inhibiting agent injector (3) on the other hand, the quartz excitation tank (5) is connected with an LED red light lamp (4) by virtue of an optical fiber and is connected with a quartz reaction tank (8) by virtue of a second micro peristaltic pump (6), and the quartz reaction tank (8) is connected with a fluorescence spectrum detector (10) by virtue of an optical fiber. The device disclosed by the invention can be used for continuously detecting the fluorescence decay of blank alga samples and alga samples with an alga inhibiting agent by virtue of the alga inhibiting agent injector, and can be used for characterizing the influence degree of the alga inhibiting agent on floating algae by virtue of integrals of attenuation of photons emitted within 90 seconds. According to the device disclosed by the invention, delayed fluorescence for characterizing alga growth inhibition can be rapidly measured, the precision and reproducibility of experiments can be improved, and the consumption of the alga inhibiting agent can be saved.
Description
Technical field
The present invention relates to a kind of device of substance-measuring, the mensure device of planktonic algae in more particularly, to a kind of water, specifically
It is related to the device that a kind of quick mensure algae is subject to algae-inhibiting agent influence degree.
Background technology
The excess emissions of the higher sewage of nitrogen and phosphorus content, lead to the aggravation of body eutrophication, wawter bloom algae frequently quick-fried
Send out the focus becoming global concern, the suppression of planktonic algae growth is the key solving water pollution problem, is also whole world phase
Close the emphasis of scholar's research.The birth of algae-inhibiting agent few in number makes the growth of algae obtain a certain degree of suppression.At present,
Sign algae that can be rough by the detection of frustule quantity and chlorophyll a is subject to algae-inhibiting agent effect.But, this
Kind detection process is complicated, speed is slow, reagent consumption amount is big.
Content of the invention
The technical problem to be solved is exactly to provide a kind of device measuring the decay of algae delayed fluorescence, and it is in inspection
It is avoided that the impact that decline frustule causes during survey, reach and characterize the degree that algae-inhibiting agent suppresses to algal grown exactly, overcome
The problems such as in prior art, detection process is complicated, speed is slow, algae-inhibiting agent consumption is big.
The technical problem to be solved is realized by such technical scheme, and it includes half flowing algae
Culture medium, algae-inhibiting agent syringe, led red globe lamp, quartz excite pond, quartz reaction pond, fluorescence spectrum detector and integrator, put
Half flowing algae media in simple glass bottle passes through the first miniature peristaltic pump connecting tee valve, and on the one hand three-way valve is connected
Quartz excites pond, on the other hand accesses algae-inhibiting agent syringe, and quartz excites pond to connect led red globe lamp by optical fiber, and quartz excites
Pond is passed through the second miniature peristaltic pump and is connected quartz reaction pond, and quartz reaction pond connects fluorescence spectrum detector, fluorescence by optical fiber
Spectrum detection instrument electrically connects integrator.
The operation principle of the present invention is: the algae solution to be measured in half flowing algae media is evacuated to stone by the first miniature peristaltic pump
English excites pond, and quartz excites pond to be connected by optical fiber with led red globe lamp, and by the irradiation of led red globe lamp, quartz excites the algae in pond
Sample produces fluorescence；Quartz is excited the algae sample being stimulated in pond be evacuated to quartz reaction pond by the second miniature peristaltic pump again, with quartz
The fluorescence spectrum detector energy continuous detecting algae solution that reaction tank connects produces the quantity of photon after being stimulated, and records light in 90s
The change of quantum count；
Algae-inhibiting agent is added in the algae solution to be measured of flowing for the unlatching of three-way valve, and the algae solution being added with algae-inhibiting agent is subject to led red
Light lamp is evacuated to quartz reaction pond after exciting, after adding algae-inhibiting agent, the photolytic activity of planktonic algae reduces, the fluorescence meeting of the generation that is stimulated
Decay, by the attenuation change of photon in fluorescence spectrum detector record 90s, finally by integrator calculate suppressed planktonic algae and
Total amount value of delta df of the delay decaying phosphor of not suppressed planktonic algae.
Compared with prior art, the detection precision of the present invention is high, and detection speed is fast, achievable full automation, and
Algae-inhibiting agent consumption is few, it is to avoid multiple interpolation algae-inhibiting agent, the trouble repeatedly measuring so that experiment reproducible.By changing
Fluorescence spectrum detector, algae-inhibiting agent etc. can realize many measure.
Brief description
Fig. 1 is the functional structure chart of the present invention.
In figure: 1. half flowing algae media；2. the first miniature peristaltic pump；3. algae-inhibiting agent syringe；4.led red globe lamp；
5. quartz excites pond；6. the second miniature peristaltic pump；7. the micro constant-temperature case with thermostat；8. quartz reaction pond；9. three-way valve；
10. fluorescence spectrum detector；11. integrators；12. first waste liquid dump valves；13. second waste liquid dump valves；14. first waste liquids are received
Collection bottle；15. second waste collection bottles.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and examples:
As shown in figure 1, the present invention includes half flowing algae media 1, algae-inhibiting agent syringe 3, led red globe lamp 4, quartz
Excite pond 5, quartz reaction pond 8, fluorescence spectrum detector 10 and integrator 11, be placed in half flowing algae training in simple glass bottle
Foster base 1 passes through the first miniature peristaltic pump 2 connecting tee valve 9, and three-way valve 9 one aspect is connected quartz and excited pond 5, on the other hand accesses
Algae-inhibiting agent syringe 3, quartz excites pond 5 to connect led red globe lamp 4 by optical fiber, and quartz excites pond 5 to pass through the second miniature peristaltic pump
6 connection quartz reaction ponds 8, quartz reaction pond 8 connects fluorescence spectrum detector 10 by optical fiber, and fluorescence spectrum detector 10 is electrically connected
Connect integrator 11.
Described quartz reaction pond 8 is placed in the calorstat 7 with thermostat, the temperature setting of the calorstat with thermostat
For 25 DEG C ± 1, the calorstat with thermostat is made using light-proof material, has both controlled reaction temperature, makes quartz reaction again
Pond is in dark state, be easy to measure dark state under algae be stimulated produce photon numbers change.
Quartz excites equipped with the first waste liquid dump valve 12 on pond 5, equipped with the second waste liquid dump valve 13 on quartz reaction pond 8,
With realize experiment terminate after or instrument clean after the smooth delivery detection device of residual waste solution, enable detection means to carry out multiple surveys
Fixed.
First miniature peristaltic pump 2 and the second miniature peristaltic pump 6 adopt acid-proof hose, it is to avoid acid algae-inhibiting agent is to connecting tube
Corrosion, using adjustable speed, gageable miniature peristaltic pump, has pollution-free, high precision, good airproof performance, the advantage such as easy to maintain；
First miniature peristaltic pump 2 is used for aspirating algae solution to be measured from half flowing algae media 1；Second miniature peristaltic pump 6 is used for from stone
English excites pond 5 that the algae solution being stimulated is evacuated to quartz reaction pond 8.Described algae-inhibiting agent syringe 3 is by microsyringe repacking
Become, this syringe is carried out with shading treatment, reduces the decomposition of algae-inhibiting agent during the course of the reaction as far as possible, and suppression is quantified by syringe
The consumption of algae agent, algae-inhibiting agent syringe 3 realizes the interpolation of algae-inhibiting agent by the keying of three-way valve 9.The wavelength model of led red globe lamp 4
Enclose for 640nm 770nm；Described integrator 11 can be connected with computer, realizes automatically calculating.
The present invention is the stability of a set of comprehensive utilization rapidity of flow injection and continuous detecting, the device of low consumption.
It is divided into three steps measuring planktonic algae delay decaying phosphor (δ df) that algae-inhibiting agent is responded:
The first step closes connected three-way valve 9 valve of algae-inhibiting agent syringe 3, closes quartz and excites the first waste liquid on pond 5
Dump valve 12, opens second waste liquid dump valve 13 in quartz reaction pond 8, opens led red globe lamp 4, opens the first miniature peristaltic pump 2
With the second miniature peristaltic pump 6, allow algae sample to be measured excite pond 5 by the first miniature peristaltic pump 2 to quartz, excite pond 5 in quartz
In excited by led red globe lamp 4 after algae sample be evacuated to the stone in the calorstat 7 with thermostat by the second miniature peristaltic pump 6
English reaction tank 8, fluorescence spectrum detector 10 records this moment algae and is stimulated produced photon amount, obtains initial value c1.Beat
Open quartzy the first waste liquid dump valve 12 exciting on pond 5, surveyed algae solution and entered the first waste collection bottle 14, quartz reaction pond 8
The second waste liquid dump valve 13 surveyed algae solution and entered the second waste collection bottle 15.
Second step closes the first waste liquid dump valve 12 and the second waste liquid dump valve 13, and device keeps above-mentioned state, when second
After algae solution to be measured is evacuated to the quartz reaction pond 8 in the calorstat 7 with thermostat by miniature peristaltic pump 6, close the first minitype creepage
Pump 2 and the second miniature peristaltic pump 6, are recorded the change of the photon amount of algae solution in 90s quartz reaction pond 8 by fluorescence spectrum detector 10
ci, open the first waste liquid dump valve 12 and the second waste liquid dump valve 13 after 90s, surveyed algae solution and entered the first waste collection bottle
14th, the second waste collection bottle 15.
3rd step closes the first waste liquid dump valve 12 and the second waste liquid dump valve 13, opens algae-inhibiting agent syringe 3 connected
Three-way valve 9 valve, opens led red globe lamp, opens the first miniature peristaltic pump 2 and the second miniature peristaltic pump 6, makes half flowing algae training
The lasting flowing in the apparatus of algae solution to be measured in foster base 1, the algae simultaneously by algae-inhibiting agent syringe 3, algae-inhibiting agent injection flowed
In liquid, biased sample excites in pond 5 in quartz and is subject to exciting of led red globe lamp 4, by the second miniature peristaltic pump 6, quartz is excited pond 5
In after the biased sample that is stimulated is evacuated to quartz reaction pond 8, close the first miniature peristaltic pump 2 and the second miniature peristaltic pump 6, by glimmering
Light spectrum detection instrument 10 records the change a of the photon amount of algae solution in quartz reaction pond 8 in 90si, after 90s, open the first waste liquid row
Go out valve 12 and the second waste liquid dump valve 13, surveyed algae solution and entered the first waste collection bottle 14, the second waste collection bottle 15.By amassing
Divide device 11 that the photon amount of 90s is integrated.Finally draw the delay decaying phosphor value δ df that planktonic algae is affected by algae-inhibiting agent.
The first waste liquid dump valve 12 can be opened after experiment terminates or after cleaning device and the second waste liquid dump valve 13 is discharged and given up
Liquid.
Above example does not constitute the restriction to patent of the present invention, all institutes in the form of equivalent or equivalent deformation
The technical scheme obtaining, all falls within the protection domain of patent of the present invention.
Claims (4)
1. a kind of device measuring the decay of algae delayed fluorescence, is characterized in that: include half flowing algae media (1), algal control
Agent syringe (3), led red globe lamp (4), quartz excite pond (5), quartz reaction pond (8), fluorescence spectrum detector (10) and integration
Device (11), the first miniature peristaltic pump (2) connecting tee valve is passed through in half flowing algae media (1) being placed in simple glass bottle
(9), three-way valve (9) is on the one hand connected quartz and is excited pond (5), on the other hand accesses algae-inhibiting agent syringe (3), quartz excites pond
(5) led red globe lamp (4) is connected by optical fiber, quartz excites pond (5) to connect quartz reaction pond by the second miniature peristaltic pump (6)
(8), quartz reaction pond (8) connect fluorescence spectrum detector (10), fluorescence spectrum detector (10) electrical connection integration by optical fiber
Device (11).
2. a kind of device measuring the decay of algae delayed fluorescence according to claim 1 it is characterised in that: described quartz is anti-
Ying Chi (8) is placed in the calorstat (7) with thermostat.
3. according to claim 1 a kind of measure algae delayed fluorescence decay device it is characterised in that: described quartz
Excite equipped with the first waste liquid dump valve (12) on pond (5), equipped with the second waste liquid dump valve on described quartz reaction pond (8)
(13).
4. according to claim 1 a kind of measure algae delayed fluorescence decay device it is characterised in that: first is miniature compacted
Dynamic pump (2) and the second miniature peristaltic pump (6) adopt acid-proof hose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410618606.1A CN104374757B (en) | 2014-11-06 | 2014-11-06 | Device for measuring delayed fluorescence decay of algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410618606.1A CN104374757B (en) | 2014-11-06 | 2014-11-06 | Device for measuring delayed fluorescence decay of algae |
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| Publication Number | Publication Date |
|---|---|
| CN104374757A CN104374757A (en) | 2015-02-25 |
| CN104374757B true CN104374757B (en) | 2017-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410618606.1A Expired - Fee Related CN104374757B (en) | 2014-11-06 | 2014-11-06 | Device for measuring delayed fluorescence decay of algae |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3605598A1 (en) * | 1986-02-21 | 1987-08-27 | Degussa | Method and device for measuring the fluorescence decay time (afterglow time) of a fluorescing substance |
| US5757013A (en) * | 1995-12-06 | 1998-05-26 | American Research Corporation Of Virginia | Fluorescence decay measurement by calculation of inner product |
| CN1920535A (en) * | 2006-09-13 | 2007-02-28 | 哈尔滨工程大学 | Device for measuring fluorescence life time |
| CN103364378A (en) * | 2013-03-05 | 2013-10-23 | 江苏天瑞仪器股份有限公司 | Multifunctional joint sampling system for atomic fluorescence spectrometer |
-
2014
- 2014-11-06 CN CN201410618606.1A patent/CN104374757B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3605598A1 (en) * | 1986-02-21 | 1987-08-27 | Degussa | Method and device for measuring the fluorescence decay time (afterglow time) of a fluorescing substance |
| US5757013A (en) * | 1995-12-06 | 1998-05-26 | American Research Corporation Of Virginia | Fluorescence decay measurement by calculation of inner product |
| CN1920535A (en) * | 2006-09-13 | 2007-02-28 | 哈尔滨工程大学 | Device for measuring fluorescence life time |
| CN103364378A (en) * | 2013-03-05 | 2013-10-23 | 江苏天瑞仪器股份有限公司 | Multifunctional joint sampling system for atomic fluorescence spectrometer |
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| Publication number | Publication date |
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| CN104374757A (en) | 2015-02-25 |
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