CN201335829Y - Device for observing obstruction caused to seepage of groundwater by microorganisms - Google Patents
Device for observing obstruction caused to seepage of groundwater by microorganisms Download PDFInfo
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- CN201335829Y CN201335829Y CNU2009200832568U CN200920083256U CN201335829Y CN 201335829 Y CN201335829 Y CN 201335829Y CN U2009200832568 U CNU2009200832568 U CN U2009200832568U CN 200920083256 U CN200920083256 U CN 200920083256U CN 201335829 Y CN201335829 Y CN 201335829Y
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Abstract
The utility model discloses a device for observing the obstruction caused to the seepage of groundwater by microorganisms. The device comprises an experimental box body, filter screens, sampling valves, a light source, a digital camera, a control water tank, a water inlet valve and a water-discharging tank. The utility model is characterized in that the upper and the lower box walls of the experimental box body are provided with the sampling valves; filter screens are arranged at a water inlet and a water outlet of the experimental box body; porous medias are filled in the experimental box body; the experimental box body is arranged at the upper stream of the water inlet; a water storage tank is connected with a water pump; the water pump is connected with the control water tank; a water feeding pipe is connected with the experimental tank body; the experimental tank body is connected with a water discharging pipe; and a water-discharging valve is mounted on the water-discharging pipe; the water-discharging pipe is connected with the water-discharging tank; the light source and the digital camera are arranged on both sides in the thickness direction of the experimental tank body; and the digital camera is connected with a computer. The device for observing the obstruction caused to the seepage of groundwater by microorganisms can directly observe and record the propagation, the migration and the obstruction manner of the microorganisms in the porous medias, and the change of the water flowing channel, acquire the observed data, and facilitates the control of microbial activity as well as the sampling analysis.
Description
Technical field
The utility model relates to the biological clogging observation technical field of seepage action of ground water, relate more specifically to a kind of device that carries out the microorganism seepage action clogging observation, be applicable to that microorganism breeds, moves and block the observation of seepage action of ground water phenomenon in the moisture underground layer of sand of research in the layer of sand space.
Background technology
The breeding migration of microorganism in the moisture pore media of nature can cause the change of medium osmosis character, thereby the technological system that relates to seepage action of ground water is caused adverse effect.For example, underground water source type heat pump has a heat-exchange system that generally adopt in phreatic area as a kind of, in use, utilize underground water to carry out the exchange of heat, low-temperature receiver or thermal source are provided for the air-conditioning system on ground, just relate to microorganism and block the low and environmental pollution problems of the heat pump system efficiency that causes.The heat-exchanger rig of underground water source type heat pump can make air/water contact, thereby makes water carry airborne microorganism and from the organic pollutants of the system of replenishing the supply.These systems and underground water is the high-quality growth medium of microorganism normally, makes microorganism breed migration in a large number.Extract and recharge in the phreatic process, because microorganism breeds migration in containing phreatic layer of sand, soil, can block flowing of water, perhaps on the borehole wall that recharges, form biological membrane, therefore the underground water that is difficult to that major part is extracted is irritated to get back in the original stratum and is gone, and causes economically difficulty and environment damage.For underground water source type heat pump, be a general problem because material blocks the difficulty of recharging that causes, it is wherein a kind of important mechanism that microorganism is blocked.The microorganism obstruction is to be caused by the living beings that microorganism itself and ECM are assembled in the water-bearing media, all is the organic-biological matter that causes biofouling such as bacterium, fungi, saccharomycete, diatom and protozoan.Can assemble unevenly in inner these living beings of moisture pore media, for the groundwater heat pump system, especially pouring water the well borehole wall in the thin one deck medium of underground layer of sand, the microorganism alluvial can form membranaceous, reduce the speed and the flow of current greatly, have a strong impact on the operation usefulness of system.If do not understand mechanism and process that its alluvial is blocked, the formulation of the design of underground water source type heat pump, maintenance scheme all can be compared blindly so, therefore need carry out actual microorganism and block the qualitative and quantitative examination of mechanism, understand the process of microorganism at the breeding of natural water-bearing WATER AS FLOW MEDIUM circulation road, migration and obstruction water stream channel, and the water stream channel change provides test figure to the influence of microorganism for setting up the physical mathematics model.
Have not yet to see the disclosed research observation device that achieves the above object.Existing similar research observation device and method can be monitored the variation of microorganism quantity in solution, method such as the disclosed biofouling monitor of Chinese invention patent (publication number CN100389317) and monitoring or detection of biological alluvial, utilize the variation of packing material two ends water pressure to judge alluvial biological in the aqueous solution that flows into packing material, reach the purpose of monitoring bio alluvial; And the disclosed a kind of physical simulation experiment long tube sand-packed model of Chinese utility model patent (publication number CN2874021Y), simulate the percolation law of different fluid with long tube filling silica sand, be used to simulate microbial flooding quarrying oil to improve the experiment of oil recovery factor.Yet, these class methods and device are can estimate the content of microorganism in aqueous solution with the filled media overall permeability, the direct observation of microorganism concrete migration process inside water flows the zone can't be provided, and can not block mechanism research for microorganism provides enough data.Other similar device has the disclosed upflow type anaerobic biofilm reactor of Chinese utility model patent (publication number CN2626986Y), it is a kind of reactor that is used for biochemical reaction, and disclosed level rotary flow suspending carrier biofilm reactor of Chinese invention patent (publication number CN1246231C) and water body in-situ restoration method thereof, utilize the microbial degradation water pollutant, because purpose of design difference, all can't be to microorganism in the breeding of pore media water stream channel, migration and obstruction process, and water stream channel changes direct observational study is carried out in the influence of microorganism.
Summary of the invention
The purpose of this utility model is the device that has been to provide a kind of microbe ground water seepage action clogging observation, simple in structure, can directly observe microorganism in the breeding of hole water-bearing media water stream channel, migration and obstruction process, and water stream channel change the influence to microorganism.Can directly observe and write down the variation of breeding migration, obstruction mode and the water flow channel of microorganism in pore media, can obtain observation data real-time quantitative, be easy to control microbial activity, be convenient to sample analysis simultaneously, help the research of seepage action of ground water living model.
The purpose of this utility model is achieved through the following technical solutions:
A kind of device of realizing the microbe ground water seepage action clogging observation method, it comprises: the experiment casing, water-storing tank, control water tank, water pump, draining water tank, light source, digital camera and the computing machine that have the entry water delivering orifice.It is characterized in that: the experiment casing is a flat, rectangular, 1～2 centimetre of thickness, and experiment casing tank wall up and down has Sampler valves, the experiment casing is transparent, there is screen pack at inherent water inlet of experiment casing and water delivering orifice place, can adorn pore media in the experiment casing, form experimental section.The upstream of experiment casing water inlet, water-storing tank links to each other with water pump, and water pump links to each other with the control water tank, forms water supply part, is linked to each other with experiment casing water inlet by oral siphon.Experiment casing water delivering orifice links to each other with rising pipe, and the water outlet water valve is housed on the rising pipe, and rising pipe links to each other with the draining water tank, forms water discharging part.Light source, digital camera are placed on the both sides of experimental box body thickness direction, digital camera links to each other with computing machine, common composition observation unit branch, but real time record sees through the light of experiment casing (comprising pore media), obtains germs collect density and pore media density data.
A kind of method of microbe ground water seepage action clogging observation is characterized in that may further comprise the steps:
1. the pore media that adds porous in the experiment casing of observation device is such as coarse sand, fine sand and the soil of stream-laid deposit;
2. according to differently launching stress requirement of observation experiment, regulate the water level that water tank is controlled in the upstream, make and test waterflow stabilization in the casing by water pump; Light source constant illumination experiment casing, the light with the transmission of digital camera record casing stores germs collect density and pore media density data by Computer Processing;
3. water level and the nutriment of controlling water tank require to change according to observation experiment, interval certain hour (1 second～24 hours), and the digital camera document image stores, long-time running;
4. according to the requirement of observation experiment quantitative test germs collect density and pore media density, contain microbial solution and pore media sample on a small quantity, use for analyzing from the Sampler valves taking-up of testing casing;
When 5. requiring killing microorganisms according to observation experiment, close into water water valve, light source was used ultraviolet source instead, to local or whole experiment casing irradiation 5～30 minutes;
Described porous pore media refers to natural underground reservoir medium or artificial dielectrics such as beaded glass, plastic bead such as fine sand, coarse sand, soil, rubble.Such as cobble, coarse sand, fine sand and the soil of stream-laid deposit, particle diameter can be loaded into the experiment casing and exceed in 1 centimetre.
The utlity model has following advantage and effect: 1. adopt the experiment casing of light transmissive material, can directly observe and write down breeding, the migration of microorganism in the porous pore media, help the research of seepage action of ground water living model; 2. owing to adopted the digital camera logger computer to store, can obtain the distribution of microorganism in the porous pore media and the variation of pore media flow channel real-time quantitative, be easy to quantitative test front and back difference.3. by the direct killing microorganisms of ultraviolet irradiation, not needing high-temperature pressurizing to kill etc. influences the additive method of water flow simulation, easy to implement, can also reach the special comparative observation requirement of killing the subregion microorganism.4. be applicable to nature pore media and artificial pore media, such as coarse sand, fine sand and the soil of stream-laid deposit, and beaded glass, plastic bead, be suitable for the biological seepage action clogging problem of modeling effort groundwater heat pump system.
Description of drawings
Fig. 1 is a kind of microbe ground water seepage action clogging observation device synoptic diagram.
Fig. 2 is that figure one microbe ground water seepage action clogging observation device box A partly is to view.
Embodiment
Below in conjunction with accompanying drawing the utility model is described in further detail:
A kind of device of microbe ground water seepage action clogging observation of realizing is made up of experiment casing 1, first filter screen 2, second filter screen 3, Sampler valves 4, light source 5, digital camera 6, computing machine 7, control water tank 8, water pump 9, water-storing tank 10, oral siphon 11, inlet valve 12, rising pipe 13 and draining water tank 14.
As shown in Figure 1 and Figure 2, experiment casing 1 has water inlet and water delivering orifice, in experiment casing 1 inside apart from water inlet and water delivering orifice 1～2 centimeters device first filter screen 2, second filter screen 3, the pore media of experiment usefulness can be seated between first filter screen 2, second filter screen 3, is limited by first filter screen 2 and second filter screen 3.Experiment casing 1 is made flat, rectangular, 1～2 centimetre of thickness, and device has Sampler valves 4 on experiment casing 1 wall, can take out aqueous solution and pore media sample from box house by Sampler valves 4 when needed.Digital camera 6 links to each other with computing machine 7, is placed on experiment casing 1 one sides (thickness direction), and light source 5 is placed on opposite side.Oral siphon 11 links to each other with experiment casing 1, control water tank 8 respectively, and water is imported experiment casing 1.Inlet valve 12 is housed on oral siphon 11, and water pump 9 links to each other with control water tank 8, water-storing tank 10 respectively by water pipe.Control water tank 8, water pump 9, water-storing tank 10, oral siphon 11 are connected to form auxiliary water supply part, the water pump 9 control water tank 8 that from water-storing tank 10, draws water, and the height of control water level is to simulate down mobile of launching stress differently.Water-storing tank 10 also has the effect of additional microbial nutrition material (such as sucrose).Rising pipe 13 respectively with experiment casing 1, draining water tank 14, accept the aqueous solution of discharging from experiment casing 1.Rising pipe 13 and draining water tank 14 are formed auxiliary water discharging part.
During concrete enforcement, experiment casing 1 can be made with quartz glass, has ultraviolet and arrives infrared whole spectral band light transmission preferably, visible light transmissivity is more than 90%, the ultraviolet spectrum district, maximum transmission reaches more than 80%, guarantees quality of optical imaging and ultraviolet killing microorganisms effect; Experiment casing 1 can be made into 1～2 meter of length, 0.5～1 meter of height, and 1～2 centimetre of thickness, 0.25～1 centimetre of casing internal diameter thickness, the pore media that guarantees to be seated in the experiment casing has good penetrability.The casing Inner filter net can adopt stainless steel aperture 0.25*0.25mm filter screen or Swagelok health filter screen etc., and the pore media that plays restriction experiment usefulness directly contacts oral siphon and rising pipe, uniform flow's effect.Two kinds of light source employing visible light source and ultraviolet sources, when taking pictures with killing microorganisms, uses digital camera respectively, visible light source usable LED cold light source, PL-68 model flash lamp for photography such as CamLight, ultraviolet source can adopt the uviol lamp of wavelength 200～400nm, such as the high boron quartz burner of Shanghai golden light ZWX-1 type ultraviolet ray disinfector.Light source adopts reflection shield, to strengthen the uniformity coefficient of irradiate light on experiment casing 1.Digital camera 6 adopts the high-resolution model of trying one's best, and such as the α of Sony 900 digital cameras, pixel resolution reaches 6000 * 4000, and the area that every pixel can corresponding 0.25 square millimeter when 1.2 meters of experimental box body lengths provides the high spatial discrimination of trying one's best.Computing machine adopts the desktop computer or the portable machine that can be connected with digital camera.The container that control water tank 8 can adopt water surface elevation to show, the water pressure of bearing with experiments of measuring casing 1.
A kind of method of microbe ground water seepage action clogging observation the steps include:
1. the pore media that adds porous in the experiment casing of observation device is such as coarse sand, fine sand and the soil of stream-laid deposit;
2. according to differently launching stress requirement of observation experiment, regulate the water level that water tank is controlled in the upstream, make and test waterflow stabilization in the casing by water pump; Light source constant illumination experiment casing, the light with the transmission of digital camera record casing stores germs collect density and pore media density data by Computer Processing;
3. water level and the nutriment of controlling water tank require to change according to observation experiment, interval certain hour (1 second～24 hours), and the digital camera document image stores, long-time running;
4. according to the requirement of observation experiment quantitative test germs collect density and pore media density, contain microbial solution and pore media sample on a small quantity, use for analyzing from the Sampler valves taking-up of testing casing;
When 5. requiring killing microorganisms according to observation experiment, close into water water valve, light source was used ultraviolet source instead, to local or whole experiment casing irradiation 5～30 minutes;
Described porous pore media refers to natural underground reservoir media such as fine sand, coarse sand, soil, rubble, can also be the wherein a kind of of artificial dielectrics such as beaded glass, plastic bead.
The course of work of the present utility model is as follows:
1. add the pore media of porous in the experiment casing 1 of observation device, be seated between first filter screen 2, second filter screen 3;
2. water-storing tank 10 dress sterilized waters are regulated the water level of controlling water tank 8 by water pump 9, make and test waterflow stabilization in the casing 1; Light source 5 constant illumination experiment casing 1 with the light of digital camera 6 record casing transmissions, is handled storage by computing machine 7;
3. water-storing tank 10 dress contain microorganism (such as green alga, Chlorophyta; Or ground bacillus Geobacillus) aqueous solution, the water level of control water tank 8 require to change according to observation experiment pressure and regulate, interval certain hour (1 second～24 hours), and digital camera 6 document images are stored in the computing machine 7 long-time running;
4. in operational process,, close inlet valve 12, from Sampler valves 4, take out low amounts of water solution and pore media sample, use for analyzing according to the requirement of observation experiment quantitative test germs collect density and pore media density;
5. in operational process, when requiring killing microorganisms according to observation experiment, close inlet valve 12, light source 5 uses ultraviolet source, and experiment casing 1 part or whole box body were shone 5～30 minutes;
6. in operational process, draining water tank 12 takes out aqueous sample, uses for analyzing.
Claims (3)
1, a kind of device of microbe ground water seepage action clogging observation, comprise experiment casing (1), filter screen (2), Sampler valves (4), light source (5), digital camera (6), control water tank (8), inlet valve (12) and draining water tank (14), it is characterized in that: experiment casing (1) tank wall up and down has Sampler valves (4), there is screen pack (2 at experiment inherent water inlet of casing (1) and water delivering orifice place, 3), dress pore media in the experiment casing (1), the upstream of experiment casing (1) water inlet, water-storing tank (10) links to each other with water pump (9), water pump (9) links to each other with control water tank (8), oral siphon (11) links to each other with experiment casing (1), experiment casing (1) links to each other with rising pipe (13), rising pipe (13) links to each other with draining water tank (14), light source, digital camera (6) is placed on the both sides of experiment casing (1) thickness direction, and digital camera links to each other with computing machine.
2, the device of a kind of microbe ground water seepage action clogging observation according to claim 1 is characterized in that: described experiment casing (1) is a flat, rectangular, 1～2 centimetre of thickness.
3, the device of a kind of microbe ground water seepage action clogging observation according to claim 1, it is characterized in that: described oral siphon (11) links to each other with experiment casing (1), control water tank (8) respectively, inlet valve (12) is housed on oral siphon (11), and water pump (9) links to each other with control water tank (8), water-storing tank (10) respectively by water pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2009200832568U CN201335829Y (en) | 2009-01-06 | 2009-01-06 | Device for observing obstruction caused to seepage of groundwater by microorganisms |
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| Application Number | Priority Date | Filing Date | Title |
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| CNU2009200832568U CN201335829Y (en) | 2009-01-06 | 2009-01-06 | Device for observing obstruction caused to seepage of groundwater by microorganisms |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101458247B (en) * | 2009-01-06 | 2012-10-03 | 中国科学院武汉岩土力学研究所 | Microbe ground water seepage action clogging observation method and apparatus |
| CN102866093A (en) * | 2012-09-04 | 2013-01-09 | 中国农业大学 | Porous medium biological clogging simulation test device and simulation test evaluation method |
| CN103710261A (en) * | 2014-01-09 | 2014-04-09 | 重庆大学 | Online biofilm sampler for water supply pipeline and sampling method thereof |
| CN105203720A (en) * | 2015-10-29 | 2015-12-30 | 中国地质大学（北京） | Test device and test method for wind, sand and underground water interaction |
-
2009
- 2009-01-06 CN CNU2009200832568U patent/CN201335829Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101458247B (en) * | 2009-01-06 | 2012-10-03 | 中国科学院武汉岩土力学研究所 | Microbe ground water seepage action clogging observation method and apparatus |
| CN102866093A (en) * | 2012-09-04 | 2013-01-09 | 中国农业大学 | Porous medium biological clogging simulation test device and simulation test evaluation method |
| CN102866093B (en) * | 2012-09-04 | 2014-12-10 | 中国农业大学 | Porous medium biological clogging simulation test device and simulation test evaluation method |
| CN103710261A (en) * | 2014-01-09 | 2014-04-09 | 重庆大学 | Online biofilm sampler for water supply pipeline and sampling method thereof |
| CN103710261B (en) * | 2014-01-09 | 2015-04-01 | 重庆大学 | Online biofilm sampler for water supply pipeline and sampling method thereof |
| CN105203720A (en) * | 2015-10-29 | 2015-12-30 | 中国地质大学（北京） | Test device and test method for wind, sand and underground water interaction |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091028 Termination date: 20150106 |
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| EXPY | Termination of patent right or utility model |