CN215727009U

CN215727009U - Device for moving liquid sample

Info

Publication number: CN215727009U
Application number: CN202121902782.XU
Authority: CN
Inventors: 祁广巍
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-08-15
Filing date: 2021-08-15
Publication date: 2022-02-01
Anticipated expiration: 2031-08-15

Abstract

The utility model discloses a device for moving and taking a liquid sample, which comprises a sample adding groove, an underframe, a swinging and uniformly mixing component and a front and back uniformly mixing component, wherein the sample adding groove is supported by the underframe and is fixed on a sliding block of the front and back uniformly mixing component, and one side of the sample adding groove is connected with the swinging and uniformly mixing component; the swinging and uniformly mixing component consists of a connecting rod 1, a connecting rod 2, a rocker and a bottom rod; the front and rear mixing components consist of a crank, a connecting rod 3, a sliding chute and a sliding block; the bottom of the sample adding slot extends downwards to form a protruding hollow connecting pipe, the hollow part of the connecting pipe forms a bottom hole of the sample adding slot at the bottom of the sample adding slot, the hole opening covers the filter membrane, the connecting pipe can be hermetically connected with an air pump output pipe, air is pressed into the sample adding slot, and a mixing sample is blown and beaten. The utility model can save the steps and time for mixing the sample uniformly, can keep the liquid uniform and homogeneous, and provides a basis and guarantee for the experiment and the uniform compliance of the product for the repeated pipetting operation with long time and large amount.

Description

Device for moving liquid sample

Technical Field

The utility model relates to the field of biomedical instruments, in particular to a device for removing a liquid sample.

Background

The sample adding groove, also known as a reagent groove, is mainly used for containing the moving reagent. In the pipetting operation, if the same liquid needs to be frequently and repeatedly pipetted, especially a multi-channel pipettor or a pipetting instrument brings inconvenience in operation, and the sample adding groove is a small tool for solving the problem, so that the pipetting is simple and convenient.

Sample application wells are commonly used in cell biology assays. Cell biology experiments were cell-based. The cell separation plate and the cell plating plate are basic technologies commonly used in cell biology experiments and also guarantee accurate experiment results, small errors and high repeatability. The sample adding grooves supplied in the current market are generally divided into a V-shaped sample adding groove and a U-shaped sample adding groove, and the groove is made into the U-shaped sample adding groove or the V-shaped sample adding groove in a rectangular box, so that the sample adding groove is suitable for repeated liquid transferring operation of a multi-channel liquid transferring device (commonly called as a discharging gun). However, in the case of the experiment for pipetting a cell suspension, since cells gradually sink in the solution, the cells accumulate more and more at the bottom of the well as the number of times of repeated pipetting increases, which tends to cause the number of cells contained in each pipetting to be uneven. The end result is uneven cell plating or plating, and the experimental results presented are not comparable or convincing.

The main method for overcoming the above problems at present is to mix the cell suspension again after adding several samples by the sample loader. The drawbacks of this operation are: firstly, it is time-consuming and labor-consuming. Pipetting is generally carried out more than 5 times, and the cell suspension is usually repeatedly blown 50 to 100 times each time for thorough mixing. If the cells are added in 384-well plates, the cells are mixed more than three hundred times after each addition of one plate. Secondly, the blowing force is difficult to balance. On one hand, the force is uneven due to fatigue of operators caused by repeated blowing; on the other hand, the liquid rushes out of the sample adding slot due to too large force in hundreds of blowing and beating processes, so that the risk of pollution is increased. Therefore, the utility model discloses equipment which is time-saving, labor-saving and capable of uniformly adding samples, and is the most basic and urgent in the field.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem of inventing equipment which is time-saving, labor-saving and capable of uniformly adding samples.

Aiming at the defects and shortcomings of the prior art, the device for moving and taking the liquid sample comprises a sample adding groove 1 and an underframe, wherein the sample adding groove is supported by the underframe and is connected with a swinging component. The swinging component comprises a swinging and uniformly mixing component 31, and the sample adding slot 1 is connected with the underframe through the swinging and uniformly mixing component 31; the swinging and mixing component 31 is composed of a connecting rod 1, a connecting rod 2, a rocker and a bottom rod 312.

Wherein, connecting rod 1 is rotated by motor drive, and bottom bar 312 is fixed connecting rod 1 and rocker 313, and rocker 313 and connecting rod 2 and sample feeding groove side fixed connection, through the reciprocal swing of drive of connecting rod 2, the other end of connecting rod 2 is connected with connecting rod 1.

Preferably, the device also comprises a front blending part and a rear blending part. The sample adding groove is fixed on the slide block of the front and back blending parts, and one side of the sample adding groove is connected with the swinging blending part.

The size of the sample adding groove can be determined according to actual needs, for example, 5-100mL, and is matched with the sample adding groove of the existing cell culture medium. Or the sample adding groove for forming the liquid sample can be constructed by laying and covering waterproof materials on the basis of the existing sample adding groove, or the sample adding groove structure can be formed at one time and used once.

The length of the sample adding groove is usually not less than 10cm, so that a discharging gun can conveniently take liquid, and a sample storage container with an appropriate size can also be taken or selected according to the equipment for taking samples.

The front and rear mixing components are composed of a crank, a connecting rod 3, a sliding groove and a sliding block, wherein the crank is driven by a motor to rotate for 360 degrees, and two ends of the connecting rod 3 are respectively fixed on the crank and the sliding block and can drive the sliding block to reciprocate in the sliding groove.

The bottom of the sample adding groove 1 extends downwards to form a protruding hollow connecting pipe 12, a hollow part of the connecting pipe 12 forms a sample adding groove bottom hole 11 at the bottom of the sample adding groove, a hole opening covers a filter membrane to prevent sample pollution, the connecting pipe 12 can be connected with an air pump output pipe in a sealing mode, air is pressed into the sample adding groove 1, and a mixed sample is blown into the sample adding groove 1 from the hollow part through the sample adding groove bottom hole 11.

Preferably, the slider 323 is i-shaped.

Preferably, the connecting tube 12 and the filter membrane are also filled with a filter material.

Preferably, the filter material is cotton.

Preferably, the filter material may also be a molecular sieve.

Preferably, the sample adding groove is hung on the bracket or fixed on the bottom frame through a movable connecting structure.

When the liquid sample sampling device is used, a liquid sample is placed in the sampling slot, and the sampling slot is installed or fixed on the bottom frame. The swinging device is started, and the swinging blending part can be started independently, the front blending part and the rear blending part can be started independently, or the front blending part and the rear blending part can be started simultaneously. And after the liquid sample is uniformly mixed, sampling.

Due to the adoption of the technical scheme, compared with the prior art, the utility model has the following advantages and positive effects: the utility model can simply and conveniently repeatedly move the same liquid sample in the liquid moving operation, saves time and labor and can uniformly sample. Especially for the repeated pipetting operation with long time and large amount, the method not only can save the step and time for uniformly mixing the samples, but also can keep the liquid uniform and homogeneous, thereby providing a foundation and guarantee for the unified compliance of experiments and products.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention, wherein 1 is a sample loading slot, 2 is an underframe, 31 is a swinging mixing component, and 32 is a front and rear mixing component;

FIG. 2 is an enlarged schematic view of a sample addition slot according to the present invention;

FIG. 3 is a top view of the sample application well of the present invention, wherein 11 is the bottom hole of the sample application well;

FIG. 4 is an enlarged schematic view of the bottom of the sample addition slot of the present invention, wherein 12 is a connecting tube;

FIG. 5 is a schematic structural diagram of a swinging mixing component of the present invention, wherein 311 is a connecting

rod

1, 312 is a bottom rod, 313 is a rocker, and 314 is a connecting rod 2;

fig. 6 is a schematic structural view of the front and rear blending parts of the present invention, wherein 321 is a crank, 322 is a connecting rod 3, 324 is a chute, and 323 is a slider.

Detailed Description

The utility model will be described in more detail below with reference to the accompanying drawings showing embodiments of the utility model. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the utility model to those skilled in the art. In the drawings, the size and relative sizes of layers and regions may be exaggerated for clarity.

Example 1

The utility model provides a device for moving and taking a liquid sample, which comprises a sample adding slot 1, an underframe 2, a swinging blending component 31 and a front blending component and a rear blending component 32 as shown in figures 1 and 2. The sample addition tank 1 is supported by the base frame 2 and fixed to the slider 323 of the front and rear kneading members 32, and the swinging kneading member 31 is connected to one side of the sample addition tank 1. The volume of the sample addition slot is 50 ml, and is matched with the sample addition slot of the common cell culture solution.

When the liquid sample sampling device is used, a liquid sample is placed in the sampling slot, and the sampling slot is installed on the bottom frame. And switching on a power supply or a power device, starting the swing device before sampling, simultaneously starting the swing blending component and the front and rear blending components, and sampling after the liquid sample is uniformly blended.

Example 2

The oscillating member can be provided in various ways. One of them is as shown in fig. 5, the swing blending part 31 is composed of a connecting rod 1, a connecting rod 2, a rocker 313 and a bottom rod 312, wherein the connecting rod 1 can rotate 360 degrees by being driven by a motor, the bottom rod 312 fixes the connecting rod 1 and the rocker 313, the rocker 313 and the connecting rod 2 are fixedly connected with the side surface of the sample adding slot, the swinging can be performed to-and-fro by the driving of the connecting rod 2, and the other end of the connecting rod 2 is connected with the connecting rod 1.

Example 3

The swinging component can also comprise a back-and-forth blending component. As shown in fig. 6, the front and rear blending component 32 is composed of a crank 321, a connecting rod 3, a sliding slot 324 and a sliding block 323, wherein the crank 321 is driven by a motor to rotate 360 degrees, and two ends of the connecting rod 3 are respectively fixed on the crank 321 and the sliding block 323 to drive the sliding block 323 to reciprocate in the sliding slot 324. The slider 323 is i-shaped.

Example 4

The connection mode of the sample addition well 1 is improved based on example 1. As shown in fig. 3 and 4, the bottom of the sample adding slot 1 extends downward to form a protruding hollow connecting tube 12, the hollow part of the connecting tube 12 forms a sample adding slot bottom hole 11 at the bottom of the sample adding slot, the hole covers the filter membrane, and the connecting tube 12 and the filter membrane are filled with cotton. The connecting pipe 12 can be hermetically connected with an output pipe of an air pump, and air is pressed into the sample adding groove 1 to blow and pump the uniformly mixed sample.

Example 5

The present invention differs from example 1 in that the connecting tube 12 is filled with molecular sieve before the filter membrane.

As those skilled in the art will appreciate, the present invention may be embodied in many other specific forms without departing from the spirit or scope thereof. Although embodiments of the present invention have been described, it is to be understood that the present invention should not be limited to those embodiments but various changes and modifications can be made by one skilled in the art within the spirit and scope of the present invention as hereinafter claimed.

Claims

1. The device for moving and taking the liquid sample is characterized by comprising a sample adding groove (1), a bottom frame (2) and a swinging component, wherein the sample adding groove (1) is connected with the bottom frame through a swinging and uniformly mixing component (31); the swing blending part (31) consists of a connecting rod 1 (311), a connecting rod 2 (314), a rocker (313) and a bottom rod (312);

wherein, the connecting rod 1 (311) is driven by a motor to rotate, the bottom rod (312) fixes the connecting rod 1 (311) and a rocker (313), the rocker (313) and the connecting rod 2 (314) are fixedly connected with the side surface of the sample adding slot, the connecting rod 2 (314) is driven to swing in a reciprocating way, and the other end of the connecting rod 2 (314) is connected with the connecting rod 1 (311).

2. The device for removing a liquid sample according to claim 1, further comprising a back and forth homogenizing element (32);

the front and rear mixing parts (32) are composed of a crank (321), a connecting rod (3) (322), a sliding chute (324) and a sliding block (323);

the crank (321) is driven by a motor to rotate, and two ends of the connecting rod (3) (322) are respectively fixed on the crank (321) and the sliding block (323) to drive the sliding block (323) to reciprocate in the sliding groove (324).

3. The apparatus for removing a liquid sample according to claim 2, wherein the sample addition well (1) is supported by the base frame (2) and fixed to a slider (323) of the front and rear kneading members (32), and the swinging kneading member (31) is connected to one side of the sample addition well (1).

4. Device for removing liquid samples according to claim 1, characterized in that the slide (323) is i-shaped.

5. Device for removing liquid samples according to claim 2, characterized in that the oscillating mixer part (31) and/or the front and back mixer parts (32) are driven by batteries or new energy sources.

6. The device for removing a liquid sample of claim 2, wherein the amplitude of rotation of the connecting rod 1 (311) or crank is up to 360 degrees.

7. Device for removing liquid samples according to any of claims 1-6, characterized in that the bottom of the sample well (1) extends downwards with a protruding hollow connecting tube (12), the hollow part of the connecting tube (12) forming a well (11) at the bottom of the sample well, which well covers the filter membrane.

8. Device for removing a liquid sample according to claim 7, characterised in that the connecting tube (12) and the filter membrane are also filled with a filter material.

9. The device for removing a liquid sample of claim 7, wherein the filter material is cotton or molecular sieve.

10. The device for removing a liquid sample according to claim 7, wherein the connecting tube (12) is hermetically connected to an output tube of an air pump, and air is pressed into the sample addition tank (1) to blow and mix the sample.